CN1114694C - 赋予植物抗病性的方法和材料 - Google Patents
赋予植物抗病性的方法和材料 Download PDFInfo
- Publication number
- CN1114694C CN1114694C CN96191493A CN96191493A CN1114694C CN 1114694 C CN1114694 C CN 1114694C CN 96191493 A CN96191493 A CN 96191493A CN 96191493 A CN96191493 A CN 96191493A CN 1114694 C CN1114694 C CN 1114694C
- Authority
- CN
- China
- Prior art keywords
- gene
- plant
- dna
- sequence
- promotor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000034 method Methods 0.000 title claims abstract description 47
- 208000035240 Disease Resistance Diseases 0.000 title description 8
- 239000000463 material Substances 0.000 title description 4
- 108090000623 proteins and genes Proteins 0.000 claims description 118
- 241000196324 Embryophyta Species 0.000 claims description 89
- 235000007164 Oryza sativa Nutrition 0.000 claims description 27
- 239000002253 acid Substances 0.000 claims description 26
- 235000009566 rice Nutrition 0.000 claims description 26
- 239000002777 nucleoside Substances 0.000 claims description 25
- 150000003833 nucleoside derivatives Chemical class 0.000 claims description 25
- 102000039446 nucleic acids Human genes 0.000 claims description 21
- 108020004707 nucleic acids Proteins 0.000 claims description 21
- 150000007523 nucleic acids Chemical class 0.000 claims description 21
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 17
- 229920001184 polypeptide Polymers 0.000 claims description 16
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 16
- 108091033319 polynucleotide Proteins 0.000 claims description 13
- 102000040430 polynucleotide Human genes 0.000 claims description 13
- 239000002157 polynucleotide Substances 0.000 claims description 13
- 201000010099 disease Diseases 0.000 claims description 12
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 12
- 235000013311 vegetables Nutrition 0.000 claims description 11
- 238000003259 recombinant expression Methods 0.000 claims description 5
- 241000209094 Oryza Species 0.000 claims 2
- 241000589636 Xanthomonas campestris Species 0.000 claims 1
- 230000000735 allogeneic effect Effects 0.000 claims 1
- 239000000203 mixture Substances 0.000 abstract description 5
- 230000008569 process Effects 0.000 abstract description 2
- 150000003839 salts Chemical class 0.000 abstract description 2
- 229910052717 sulfur Inorganic materials 0.000 abstract description 2
- 125000003710 aryl alkyl group Chemical group 0.000 abstract 2
- 125000001183 hydrocarbyl group Chemical group 0.000 abstract 2
- 229910052739 hydrogen Inorganic materials 0.000 abstract 2
- 239000001257 hydrogen Substances 0.000 abstract 2
- 125000004435 hydrogen atom Chemical group [H]* 0.000 abstract 2
- 230000003308 immunostimulating effect Effects 0.000 abstract 2
- WJSVJNDMOQTICG-UHFFFAOYSA-N 2-amino-1-[(2-methyl-4-methylidene-5-oxooxolan-2-yl)methyl]-7h-purin-6-one Chemical class NC1=NC=2N=CNC=2C(=O)N1CC1(C)CC(=C)C(=O)O1 WJSVJNDMOQTICG-UHFFFAOYSA-N 0.000 abstract 1
- 125000002252 acyl group Chemical group 0.000 abstract 1
- 125000004432 carbon atom Chemical group C* 0.000 abstract 1
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical class O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 abstract 1
- 125000002757 morpholinyl group Chemical group 0.000 abstract 1
- 229910052760 oxygen Inorganic materials 0.000 abstract 1
- 125000001424 substituent group Chemical group 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 description 69
- 240000007594 Oryza sativa Species 0.000 description 29
- 210000004436 artificial bacterial chromosome Anatomy 0.000 description 26
- 210000004027 cell Anatomy 0.000 description 23
- 239000012634 fragment Substances 0.000 description 22
- 238000009396 hybridization Methods 0.000 description 22
- 241000894006 Bacteria Species 0.000 description 21
- 238000005516 engineering process Methods 0.000 description 20
- 241000227653 Lycopersicon Species 0.000 description 19
- 239000000523 sample Substances 0.000 description 18
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 17
- 239000012528 membrane Substances 0.000 description 17
- 102000004169 proteins and genes Human genes 0.000 description 17
- 239000000243 solution Substances 0.000 description 17
- 235000018102 proteins Nutrition 0.000 description 16
- 238000003752 polymerase chain reaction Methods 0.000 description 15
- 229940024606 amino acid Drugs 0.000 description 14
- 235000001014 amino acid Nutrition 0.000 description 14
- 150000001413 amino acids Chemical class 0.000 description 13
- 239000002299 complementary DNA Substances 0.000 description 13
- 108091000080 Phosphotransferase Proteins 0.000 description 11
- 238000006243 chemical reaction Methods 0.000 description 11
- 102000020233 phosphotransferase Human genes 0.000 description 11
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 10
- 238000013016 damping Methods 0.000 description 10
- 239000012530 fluid Substances 0.000 description 10
- 125000003275 alpha amino acid group Chemical group 0.000 description 9
- 230000003321 amplification Effects 0.000 description 9
- 239000000499 gel Substances 0.000 description 9
- 238000003199 nucleic acid amplification method Methods 0.000 description 9
- 238000002360 preparation method Methods 0.000 description 9
- 239000000047 product Substances 0.000 description 9
- 101100165929 Arabidopsis thaliana CRK6 gene Proteins 0.000 description 8
- 101100037317 Arabidopsis thaliana RLK5 gene Proteins 0.000 description 8
- 241000589649 Xanthomonas campestris pv. campestris Species 0.000 description 8
- 239000004927 clay Substances 0.000 description 8
- 238000010276 construction Methods 0.000 description 8
- 230000029087 digestion Effects 0.000 description 8
- 239000002773 nucleotide Substances 0.000 description 8
- 125000003729 nucleotide group Chemical group 0.000 description 8
- 230000009466 transformation Effects 0.000 description 8
- 229920000936 Agarose Polymers 0.000 description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 7
- 239000003550 marker Substances 0.000 description 7
- 238000000926 separation method Methods 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 210000001519 tissue Anatomy 0.000 description 7
- 230000009261 transgenic effect Effects 0.000 description 7
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 6
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 6
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 6
- 230000008859 change Effects 0.000 description 6
- 238000004520 electroporation Methods 0.000 description 6
- 230000002068 genetic effect Effects 0.000 description 6
- 230000004048 modification Effects 0.000 description 6
- 238000012986 modification Methods 0.000 description 6
- 238000001556 precipitation Methods 0.000 description 6
- 230000008929 regeneration Effects 0.000 description 6
- 238000011069 regeneration method Methods 0.000 description 6
- 238000012216 screening Methods 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 241000589158 Agrobacterium Species 0.000 description 5
- 108700001094 Plant Genes Proteins 0.000 description 5
- 239000003795 chemical substances by application Substances 0.000 description 5
- 230000000295 complement effect Effects 0.000 description 5
- 238000003780 insertion Methods 0.000 description 5
- 230000037431 insertion Effects 0.000 description 5
- 108020004999 messenger RNA Proteins 0.000 description 5
- 230000001717 pathogenic effect Effects 0.000 description 5
- 239000013612 plasmid Substances 0.000 description 5
- 238000003906 pulsed field gel electrophoresis Methods 0.000 description 5
- 238000012546 transfer Methods 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 4
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 4
- 108091028043 Nucleic acid sequence Proteins 0.000 description 4
- 241000589634 Xanthomonas Species 0.000 description 4
- 241000209149 Zea Species 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 4
- 238000010367 cloning Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000001962 electrophoresis Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 238000006062 fragmentation reaction Methods 0.000 description 4
- 230000006870 function Effects 0.000 description 4
- 230000012010 growth Effects 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 231100000252 nontoxic Toxicity 0.000 description 4
- 230000003000 nontoxic effect Effects 0.000 description 4
- 229940049547 paraxin Drugs 0.000 description 4
- 239000000843 powder Substances 0.000 description 4
- 210000001938 protoplast Anatomy 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 108091008146 restriction endonucleases Proteins 0.000 description 4
- ATHGHQPFGPMSJY-UHFFFAOYSA-N spermidine Chemical compound NCCCCNCCCN ATHGHQPFGPMSJY-UHFFFAOYSA-N 0.000 description 4
- PFNFFQXMRSDOHW-UHFFFAOYSA-N spermine Chemical compound NCCCNCCCCNCCCN PFNFFQXMRSDOHW-UHFFFAOYSA-N 0.000 description 4
- 238000010561 standard procedure Methods 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 108010013043 Acetylesterase Proteins 0.000 description 3
- 239000004475 Arginine Substances 0.000 description 3
- 241000193830 Bacillus <bacterium> Species 0.000 description 3
- 108020004705 Codon Proteins 0.000 description 3
- 108010067770 Endopeptidase K Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 241000233866 Fungi Species 0.000 description 3
- 239000004471 Glycine Substances 0.000 description 3
- 241000209510 Liliopsida Species 0.000 description 3
- 108050002122 Protein kinase domains Proteins 0.000 description 3
- 102000012515 Protein kinase domains Human genes 0.000 description 3
- 241000589615 Pseudomonas syringae Species 0.000 description 3
- 108091005682 Receptor kinases Proteins 0.000 description 3
- 108020004511 Recombinant DNA Proteins 0.000 description 3
- 241000209056 Secale Species 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 235000002634 Solanum Nutrition 0.000 description 3
- 241000207763 Solanum Species 0.000 description 3
- 229930006000 Sucrose Natural products 0.000 description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
- 101150019400 TMK1 gene Proteins 0.000 description 3
- 101710097146 Uncharacterized protein HKLF1 Proteins 0.000 description 3
- -1 Xie Ansuan Chemical compound 0.000 description 3
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 3
- 238000000137 annealing Methods 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 230000008034 disappearance Effects 0.000 description 3
- YQYJSBFKSSDGFO-FWAVGLHBSA-N hygromycin A Chemical compound O[C@H]1[C@H](O)[C@H](C(=O)C)O[C@@H]1Oc1ccc(\C=C(/C)C(=O)N[C@@H]2[C@@H]([C@H]3OCO[C@H]3[C@@H](O)[C@@H]2O)O)cc1O YQYJSBFKSSDGFO-FWAVGLHBSA-N 0.000 description 3
- 230000000968 intestinal effect Effects 0.000 description 3
- 230000003902 lesion Effects 0.000 description 3
- 210000004940 nucleus Anatomy 0.000 description 3
- 210000000056 organ Anatomy 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 239000005720 sucrose Substances 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 241000207875 Antirrhinum Species 0.000 description 2
- 241000219194 Arabidopsis Species 0.000 description 2
- 101100061426 Arabidopsis thaliana CRK10 gene Proteins 0.000 description 2
- 101100309717 Arabidopsis thaliana SD22 gene Proteins 0.000 description 2
- 244000003416 Asparagus officinalis Species 0.000 description 2
- 235000005340 Asparagus officinalis Nutrition 0.000 description 2
- 241001106067 Atropa Species 0.000 description 2
- 235000005781 Avena Nutrition 0.000 description 2
- 244000075850 Avena orientalis Species 0.000 description 2
- 235000002566 Capsicum Nutrition 0.000 description 2
- 240000008574 Capsicum frutescens Species 0.000 description 2
- 241000701489 Cauliflower mosaic virus Species 0.000 description 2
- 241000207199 Citrus Species 0.000 description 2
- 108091026890 Coding region Proteins 0.000 description 2
- 108091035707 Consensus sequence Proteins 0.000 description 2
- 244000024469 Cucumis prophetarum Species 0.000 description 2
- 235000010071 Cucumis prophetarum Nutrition 0.000 description 2
- 238000007399 DNA isolation Methods 0.000 description 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
- 241000208175 Daucus Species 0.000 description 2
- 240000001879 Digitalis lutea Species 0.000 description 2
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 2
- 241000220223 Fragaria Species 0.000 description 2
- 241000208152 Geranium Species 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- 241000208818 Helianthus Species 0.000 description 2
- 241000209219 Hordeum Species 0.000 description 2
- 206010020649 Hyperkeratosis Diseases 0.000 description 2
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 2
- 241000208822 Lactuca Species 0.000 description 2
- 102000003960 Ligases Human genes 0.000 description 2
- 108090000364 Ligases Proteins 0.000 description 2
- 241000208204 Linum Species 0.000 description 2
- 241000209082 Lolium Species 0.000 description 2
- 240000003183 Manihot esculenta Species 0.000 description 2
- 241000219823 Medicago Species 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 240000002853 Nelumbo nucifera Species 0.000 description 2
- 235000006508 Nelumbo nucifera Nutrition 0.000 description 2
- 235000006510 Nelumbo pentapetala Nutrition 0.000 description 2
- 241000208125 Nicotiana Species 0.000 description 2
- 206010034133 Pathogen resistance Diseases 0.000 description 2
- 241000208181 Pelargonium Species 0.000 description 2
- 241000209046 Pennisetum Species 0.000 description 2
- 241000219833 Phaseolus Species 0.000 description 2
- 241000219843 Pisum Species 0.000 description 2
- 102000001253 Protein Kinase Human genes 0.000 description 2
- 108010009341 Protein Serine-Threonine Kinases Proteins 0.000 description 2
- 102000009516 Protein Serine-Threonine Kinases Human genes 0.000 description 2
- 101100083082 Pyrus communis PGIP gene Proteins 0.000 description 2
- 241000218206 Ranunculus Species 0.000 description 2
- 241000780602 Senecio Species 0.000 description 2
- 240000006394 Sorghum bicolor Species 0.000 description 2
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 2
- 108700026226 TATA Box Proteins 0.000 description 2
- 241001312519 Trigonella Species 0.000 description 2
- 229920004890 Triton X-100 Polymers 0.000 description 2
- 241000219977 Vigna Species 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 125000000539 amino acid group Chemical group 0.000 description 2
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 2
- 239000002585 base Substances 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 239000003139 biocide Substances 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 239000001390 capsicum minimum Substances 0.000 description 2
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 210000003763 chloroplast Anatomy 0.000 description 2
- 230000002759 chromosomal effect Effects 0.000 description 2
- 235000020971 citrus fruits Nutrition 0.000 description 2
- 238000003776 cleavage reaction Methods 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 230000000875 corresponding effect Effects 0.000 description 2
- 230000001086 cytosolic effect Effects 0.000 description 2
- 238000000502 dialysis Methods 0.000 description 2
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 2
- 229960005542 ethidium bromide Drugs 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 238000013467 fragmentation Methods 0.000 description 2
- 230000008676 import Effects 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 229960000310 isoleucine Drugs 0.000 description 2
- 229930182817 methionine Natural products 0.000 description 2
- 239000004570 mortar (masonry) Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 230000026731 phosphorylation Effects 0.000 description 2
- 238000006366 phosphorylation reaction Methods 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 230000008488 polyadenylation Effects 0.000 description 2
- 230000004853 protein function Effects 0.000 description 2
- 108060006633 protein kinase Proteins 0.000 description 2
- 230000008521 reorganization Effects 0.000 description 2
- 230000007017 scission Effects 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 125000003607 serino group Chemical group [H]N([H])[C@]([H])(C(=O)[*])C(O[H])([H])[H] 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 229940063673 spermidine Drugs 0.000 description 2
- 229940063675 spermine Drugs 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 230000005026 transcription initiation Effects 0.000 description 2
- 238000001890 transfection Methods 0.000 description 2
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- 239000005418 vegetable material Substances 0.000 description 2
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 2
- MEIRRNXMZYDVDW-MQQKCMAXSA-N (2E,4E)-2,4-hexadien-1-ol Chemical compound C\C=C\C=C\CO MEIRRNXMZYDVDW-MQQKCMAXSA-N 0.000 description 1
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 1
- WNBBONCQSHHDII-BHFSHLQUSA-N (2s)-2,4-diamino-4-oxobutanoic acid;(2s)-2,5-diamino-5-oxopentanoic acid Chemical compound OC(=O)[C@@H](N)CC(N)=O.OC(=O)[C@@H](N)CCC(N)=O WNBBONCQSHHDII-BHFSHLQUSA-N 0.000 description 1
- BEJKOYIMCGMNRB-GRHHLOCNSA-N (2s)-2-amino-3-(4-hydroxyphenyl)propanoic acid;(2s)-2-amino-3-phenylpropanoic acid Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1.OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 BEJKOYIMCGMNRB-GRHHLOCNSA-N 0.000 description 1
- YUXKOWPNKJSTPQ-AXWWPMSFSA-N (2s,3r)-2-amino-3-hydroxybutanoic acid;(2s)-2-amino-3-hydroxypropanoic acid Chemical compound OC[C@H](N)C(O)=O.C[C@@H](O)[C@H](N)C(O)=O YUXKOWPNKJSTPQ-AXWWPMSFSA-N 0.000 description 1
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- ZBMRKNMTMPPMMK-UHFFFAOYSA-N 2-amino-4-[hydroxy(methyl)phosphoryl]butanoic acid;azane Chemical compound [NH4+].CP(O)(=O)CCC(N)C([O-])=O ZBMRKNMTMPPMMK-UHFFFAOYSA-N 0.000 description 1
- OPIFSICVWOWJMJ-AEOCFKNESA-N 5-bromo-4-chloro-3-indolyl beta-D-galactoside Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=CNC2=CC=C(Br)C(Cl)=C12 OPIFSICVWOWJMJ-AEOCFKNESA-N 0.000 description 1
- 241000219195 Arabidopsis thaliana Species 0.000 description 1
- 101100393868 Arabidopsis thaliana GT11 gene Proteins 0.000 description 1
- 241000228439 Bipolaris zeicola Species 0.000 description 1
- 108010006654 Bleomycin Proteins 0.000 description 1
- 241000219198 Brassica Species 0.000 description 1
- 235000011331 Brassica Nutrition 0.000 description 1
- 101100043398 Brassica oleracea var. viridis SRK6 gene Proteins 0.000 description 1
- 241000209200 Bromus Species 0.000 description 1
- 101100419062 Caenorhabditis elegans rps-2 gene Proteins 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 241000723343 Cichorium Species 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 241000208296 Datura Species 0.000 description 1
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 1
- 241000283074 Equus asinus Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 101000896145 Escherichia phage Mu Baseplate hub protein gp44 Proteins 0.000 description 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- GVGLGOZIDCSQPN-PVHGPHFFSA-N Heroin Chemical compound O([C@H]1[C@H](C=C[C@H]23)OC(C)=O)C4=C5[C@@]12CCN(C)[C@@H]3CC5=CC=C4OC(C)=O GVGLGOZIDCSQPN-PVHGPHFFSA-N 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 101001059454 Homo sapiens Serine/threonine-protein kinase MARK2 Proteins 0.000 description 1
- 241000208278 Hyoscyamus Species 0.000 description 1
- 241001562081 Ikeda Species 0.000 description 1
- 241000758789 Juglans Species 0.000 description 1
- 235000013757 Juglans Nutrition 0.000 description 1
- 240000004929 Juglans cinerea Species 0.000 description 1
- 235000014056 Juglans cinerea Nutrition 0.000 description 1
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 1
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 1
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- 235000002262 Lycopersicon Nutrition 0.000 description 1
- 241001300479 Macroptilium Species 0.000 description 1
- 241001330975 Magnaporthe oryzae Species 0.000 description 1
- 241000121629 Majorana Species 0.000 description 1
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 description 1
- 208000031888 Mycoses Diseases 0.000 description 1
- XUYPXLNMDZIRQH-LURJTMIESA-N N-acetyl-L-methionine Chemical compound CSCC[C@@H](C(O)=O)NC(C)=O XUYPXLNMDZIRQH-LURJTMIESA-N 0.000 description 1
- 206010028851 Necrosis Diseases 0.000 description 1
- 241001282315 Nemesis Species 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 238000000636 Northern blotting Methods 0.000 description 1
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 1
- 241000219830 Onobrychis Species 0.000 description 1
- 240000003010 Oryza longistaminata Species 0.000 description 1
- 235000007189 Oryza longistaminata Nutrition 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 241000209117 Panicum Species 0.000 description 1
- 235000006443 Panicum miliaceum subsp. miliaceum Nutrition 0.000 description 1
- 235000009037 Panicum miliaceum subsp. ruderale Nutrition 0.000 description 1
- 241000222291 Passalora fulva Species 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 240000007377 Petunia x hybrida Species 0.000 description 1
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 1
- 108010076504 Protein Sorting Signals Proteins 0.000 description 1
- 101100492348 Pseudomonas syringae pv. tomato avrRpt2 gene Proteins 0.000 description 1
- 241000220259 Raphanus Species 0.000 description 1
- 101710140185 Receptor-like protein kinase Proteins 0.000 description 1
- 241000220010 Rhode Species 0.000 description 1
- 102000001332 SRC Human genes 0.000 description 1
- 108060006706 SRC Proteins 0.000 description 1
- 101100047214 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) TRL1 gene Proteins 0.000 description 1
- 241001106018 Salpiglossis Species 0.000 description 1
- 206010039491 Sarcoma Diseases 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- 102100028904 Serine/threonine-protein kinase MARK2 Human genes 0.000 description 1
- 241000220261 Sinapis Species 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 101150020412 TRL1 gene Proteins 0.000 description 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
- 239000004473 Threonine Substances 0.000 description 1
- 108700009124 Transcription Initiation Site Proteins 0.000 description 1
- 241000219793 Trifolium Species 0.000 description 1
- 241000005601 Trisetum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- 239000006035 Tryptophane Substances 0.000 description 1
- 108060008682 Tumor Necrosis Factor Proteins 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 241001272684 Xanthomonas campestris pv. oryzae Species 0.000 description 1
- 241000589652 Xanthomonas oryzae Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 125000001931 aliphatic group Chemical group 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 125000004390 alkyl sulfonyl group Chemical group 0.000 description 1
- 150000001408 amides Chemical group 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 238000013475 authorization Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 125000001797 benzyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])* 0.000 description 1
- 230000027455 binding Effects 0.000 description 1
- 238000012742 biochemical analysis Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229960001561 bleomycin Drugs 0.000 description 1
- OYVAGSVQBOHSSS-UAPAGMARSA-O bleomycin A2 Chemical compound N([C@H](C(=O)N[C@H](C)[C@@H](O)[C@H](C)C(=O)N[C@@H]([C@H](O)C)C(=O)NCCC=1SC=C(N=1)C=1SC=C(N=1)C(=O)NCCC[S+](C)C)[C@@H](O[C@H]1[C@H]([C@@H](O)[C@H](O)[C@H](CO)O1)O[C@@H]1[C@H]([C@@H](OC(N)=O)[C@H](O)[C@@H](CO)O1)O)C=1N=CNC=1)C(=O)C1=NC([C@H](CC(N)=O)NC[C@H](N)C(N)=O)=NC(N)=C1C OYVAGSVQBOHSSS-UAPAGMARSA-O 0.000 description 1
- GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Chemical compound BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 description 1
- 210000004899 c-terminal region Anatomy 0.000 description 1
- AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 239000013601 cosmid vector Substances 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000004665 defense response Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 230000030609 dephosphorylation Effects 0.000 description 1
- 238000006209 dephosphorylation reaction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- VHJLVAABSRFDPM-ZXZARUISSA-N dithioerythritol Chemical compound SC[C@H](O)[C@H](O)CS VHJLVAABSRFDPM-ZXZARUISSA-N 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 241001233957 eudicotyledons Species 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 238000012252 genetic analysis Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 1
- 238000001738 isopycnic centrifugation Methods 0.000 description 1
- 210000004901 leucine-rich repeat Anatomy 0.000 description 1
- 230000002934 lysing effect Effects 0.000 description 1
- 239000012139 lysis buffer Substances 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 235000005739 manihot Nutrition 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000009456 molecular mechanism Effects 0.000 description 1
- VYQNWZOUAUKGHI-UHFFFAOYSA-N monobenzone Chemical compound C1=CC(O)=CC=C1OCC1=CC=CC=C1 VYQNWZOUAUKGHI-UHFFFAOYSA-N 0.000 description 1
- 230000017074 necrotic cell death Effects 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 238000007899 nucleic acid hybridization Methods 0.000 description 1
- 239000002853 nucleic acid probe Substances 0.000 description 1
- 238000006384 oligomerization reaction Methods 0.000 description 1
- 239000002751 oligonucleotide probe Substances 0.000 description 1
- 108091008819 oncoproteins Proteins 0.000 description 1
- 102000027450 oncoproteins Human genes 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 235000019833 protease Nutrition 0.000 description 1
- NHDHVHZZCFYRSB-UHFFFAOYSA-N pyriproxyfen Chemical compound C=1C=CC=NC=1OC(C)COC(C=C1)=CC=C1OC1=CC=CC=C1 NHDHVHZZCFYRSB-UHFFFAOYSA-N 0.000 description 1
- 238000000197 pyrolysis Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 101150060482 rps2 gene Proteins 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 238000001612 separation test Methods 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 230000008054 signal transmission Effects 0.000 description 1
- 150000003384 small molecules Chemical group 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000000856 sucrose gradient centrifugation Methods 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000009182 swimming Effects 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 230000014621 translational initiation Effects 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
- 229940038773 trisodium citrate Drugs 0.000 description 1
- 229960004799 tryptophan Drugs 0.000 description 1
- 239000013598 vector Substances 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 230000001018 virulence Effects 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8281—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for bacterial resistance
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US37337595A | 1995-01-17 | 1995-01-17 | |
| US08/373,374 | 1995-01-17 | ||
| US08/475,891 US5859339A (en) | 1995-01-17 | 1995-06-07 | Nucleic acids, from oryza sativa, which encode leucine-rich repeat polypeptides and enhance xanthomonas resistance in plants |
| US08/475,891 | 1995-06-07 | ||
| US464595P | 1995-09-29 | 1995-09-29 | |
| US60/004,645 | 1995-09-29 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1191573A CN1191573A (zh) | 1998-08-26 |
| CN1114694C true CN1114694C (zh) | 2003-07-16 |
Family
ID=45476045
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN96191493A Expired - Fee Related CN1114694C (zh) | 1995-01-17 | 1996-01-17 | 赋予植物抗病性的方法和材料 |
Country Status (14)
| Country | Link |
|---|---|
| EP (1) | EP0805860A2 (hu) |
| JP (1) | JPH11514206A (hu) |
| CN (1) | CN1114694C (hu) |
| AU (1) | AU710145B2 (hu) |
| BG (1) | BG62976B1 (hu) |
| BR (1) | BR9606918A (hu) |
| CA (1) | CA2210440A1 (hu) |
| GE (1) | GEP20022684B (hu) |
| HU (1) | HUP9802411A3 (hu) |
| MX (1) | MX9705580A (hu) |
| NZ (1) | NZ302843A (hu) |
| PL (1) | PL185422B1 (hu) |
| TR (1) | TR199700650T1 (hu) |
| WO (1) | WO1996022375A2 (hu) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020092041A1 (en) * | 1997-08-13 | 2002-07-11 | Pamela C. Ronald | Procedures and materials for conferring disease resistance in plants |
| US6476292B1 (en) | 1998-02-26 | 2002-11-05 | Pioneer Hi-Bred International, Inc. | Methods for enhancing disease resistance in plants |
| WO1999043821A1 (en) * | 1998-02-26 | 1999-09-02 | Pioneer Hi-Bred International, Inc. | Genes for activation of plant pathogen defense systems |
| WO1999043823A1 (en) * | 1998-02-26 | 1999-09-02 | Pioneer Hi-Bred International, Inc. | Methods for enhancing disease resistance in plants |
| JP2002505109A (ja) * | 1998-03-06 | 2002-02-19 | シンジェンタ モーヘン ビー． ブイ． | 植物における病原体抵抗性の誘導のための方法 |
| IT1299184B1 (it) * | 1998-06-08 | 2000-02-29 | Istituto Agrario Di San Michel | Sequenze nucleotidiche del gene lrpkm1 di melo, sequenze amminoacidiche e loro usi. |
| US20020108140A1 (en) * | 1998-07-17 | 2002-08-08 | Jeffrey L. Bennetzen | Compositions and methods for enhancing disease resistance in plants |
| AU6018099A (en) * | 1998-08-14 | 2000-03-06 | Kansas State University Research Foundation | Compounds useful to affect resistance in plants and methods related thereto |
| CN1114616C (zh) * | 1999-08-10 | 2003-07-16 | 南京农业大学 | 一种诱导植物过敏性反应的激发子、制备方法及其应用 |
| AU2000266120A1 (en) * | 2000-07-28 | 2002-02-13 | The Salk Institute For Biological Studies | Chimeric receptors and their uses |
| AR075466A1 (es) | 2008-10-22 | 2011-04-06 | Basf Se | Uso de herbicidas tipo auxina en plantas cultivadas |
| WO2010046423A2 (en) | 2008-10-22 | 2010-04-29 | Basf Se | Use of sulfonylurea herbicides on cultivated plants |
| CN102134602B (zh) * | 2010-12-21 | 2013-04-24 | 中国检验检疫科学研究院 | 转Xa21基因水稻或其制品检测用引物和探针、试剂盒及方法 |
| WO2014053395A1 (en) | 2012-10-01 | 2014-04-10 | Basf Se | Use of n-thio-anthranilamide compounds on cultivated plants |
| WO2014079820A1 (en) | 2012-11-22 | 2014-05-30 | Basf Se | Use of anthranilamide compounds for reducing insect-vectored viral infections |
| EP3028573A1 (en) | 2014-12-05 | 2016-06-08 | Basf Se | Use of a triazole fungicide on transgenic plants |
| WO2016091674A1 (en) | 2014-12-12 | 2016-06-16 | Basf Se | Use of cyclaniliprole on cultivated plants |
| CA2980505A1 (en) | 2015-04-07 | 2016-10-13 | Basf Agrochemical Products B.V. | Use of an insecticidal carboxamide compound against pests on cultivated plants |
| EP3338552A1 (en) | 2016-12-21 | 2018-06-27 | Basf Se | Use of a tetrazolinone fungicide on transgenic plants |
| CN114941008B (zh) * | 2022-05-25 | 2023-03-24 | 广东省农业科学院设施农业研究所 | 菜心LRR受体蛋白激酶基因BraEFR在抗霜霉病中的应用 |
-
1996
- 1996-01-17 MX MX9705580A patent/MX9705580A/es not_active IP Right Cessation
- 1996-01-17 CA CA002210440A patent/CA2210440A1/en not_active Abandoned
- 1996-01-17 PL PL96321365A patent/PL185422B1/pl not_active IP Right Cessation
- 1996-01-17 HU HU9802411A patent/HUP9802411A3/hu unknown
- 1996-01-17 TR TR97/00650T patent/TR199700650T1/xx unknown
- 1996-01-17 EP EP96905167A patent/EP0805860A2/en not_active Withdrawn
- 1996-01-17 CN CN96191493A patent/CN1114694C/zh not_active Expired - Fee Related
- 1996-01-17 AU AU48997/96A patent/AU710145B2/en not_active Ceased
- 1996-01-17 BR BR9606918A patent/BR9606918A/pt not_active Application Discontinuation
- 1996-01-17 GE GE2791A patent/GEP20022684B/en unknown
- 1996-01-17 JP JP8522397A patent/JPH11514206A/ja not_active Withdrawn
- 1996-01-17 NZ NZ302843A patent/NZ302843A/xx unknown
- 1996-01-17 WO PCT/US1996/000717 patent/WO1996022375A2/en not_active Application Discontinuation
-
1997
- 1997-08-14 BG BG101832A patent/BG62976B1/bg unknown
Non-Patent Citations (2)
| Title |
|---|
| M.L.P. ABENES ET AL.,: "Orientation and integration of the classical and Molecular Genetic Maps of Chromosome 11 in Rice", EUPHYTICA, vol. 76, 1 January 1994 (1994-01-01), XP002007227, DOI: 10.1007/BF00024023 * |
| PAMELA C. RONALD ET AL.,: "Genetic and Physical Analysis of the Rice Bacterial Blight Disease Resistance", MOL GEN GENET, vol. 236, 1 January 1992 (1992-01-01) * |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH11514206A (ja) | 1999-12-07 |
| HUP9802411A3 (en) | 2000-11-28 |
| NZ302843A (en) | 1999-11-29 |
| AU4899796A (en) | 1996-08-07 |
| BG62976B1 (bg) | 2000-12-29 |
| PL321365A1 (en) | 1997-12-08 |
| TR199700650T1 (xx) | 1998-01-21 |
| MX9705580A (es) | 1997-11-29 |
| BG101832A (en) | 1998-07-31 |
| AU710145B2 (en) | 1999-09-16 |
| WO1996022375A2 (en) | 1996-07-25 |
| CN1191573A (zh) | 1998-08-26 |
| BR9606918A (pt) | 1997-11-11 |
| CA2210440A1 (en) | 1996-07-25 |
| PL185422B1 (pl) | 2003-05-30 |
| HUP9802411A2 (hu) | 1999-01-28 |
| GEP20022684B (en) | 2002-04-25 |
| EP0805860A2 (en) | 1997-11-12 |
| WO1996022375A3 (en) | 1996-10-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1114694C (zh) | 赋予植物抗病性的方法和材料 | |
| WO2018113702A1 (en) | Plant grain trait-related protein, gene, promoter and snps and haplotypes | |
| US9434955B2 (en) | Proteins relating to grain shape and leaf shape of rice, coding genes and uses thereof | |
| CN1293711A (zh) | 编码mlo蛋白质并赋予植物抗真菌抗性的基因 | |
| EP1003843A2 (en) | Procedures and materials for conferring disease resistance in plants | |
| US5859339A (en) | Nucleic acids, from oryza sativa, which encode leucine-rich repeat polypeptides and enhance xanthomonas resistance in plants | |
| US20160024516A1 (en) | Modulation of ACC Deaminase Expression | |
| US5952485A (en) | Procedures and materials for conferring disease resistance in plants | |
| CN110818784B (zh) | 水稻基因OsATL15在调节农药的吸收转运中的应用 | |
| WO2014113605A1 (en) | Proteins involved in plant stress response | |
| CN101883572B (zh) | 高粱铝耐受基因SbMATE | |
| CN103261425A (zh) | 病毒启动子、其截短物以及使用方法 | |
| CN1379783A (zh) | 通过功能性地抑制植物细胞周期蛋白抑制剂基因增加植物细胞增殖的方法 | |
| CN1900280A (zh) | 水稻分蘖调控基因OsTIL1及其应用 | |
| CN1285872A (zh) | 与植物疾病抗性相关的基因 | |
| EP0814161A1 (en) | Genetic control of polar auxin transport in plants and manipulation of plant growth, architecture and morphogenesis | |
| US20120117686A1 (en) | Stress-tolerant plants expressing mannosylglycerate-producing enzymes | |
| US7060480B2 (en) | MRE11 orthologue and uses thereof | |
| CN1235640A (zh) | 臭氧诱导的植物基因表达 | |
| US20020086428A1 (en) | Methods and compositions for independent DNA replication in eukaryotic cells | |
| WO2000009698A2 (en) | Compounds useful to affect resistance in plants and methods related thereto | |
| RU2203320C2 (ru) | Способы и вещества для придания растениям устойчивости к заболеваниям | |
| CN118028302A (zh) | 紫花苜蓿类钙调蛋白cml44基因及其应用 | |
| CN118290557A (zh) | 水稻分蘖数量调控的Tn1a蛋白及其相关生物材料与应用 | |
| CN101998994B (zh) | 玉米aba信号基因及使用方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |