CN111454826A - Sample preparation device for detecting suspected African swine fever material in pig farm - Google Patents
Sample preparation device for detecting suspected African swine fever material in pig farm Download PDFInfo
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- CN111454826A CN111454826A CN202010490803.5A CN202010490803A CN111454826A CN 111454826 A CN111454826 A CN 111454826A CN 202010490803 A CN202010490803 A CN 202010490803A CN 111454826 A CN111454826 A CN 111454826A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 23
- 208000007407 African swine fever Diseases 0.000 title claims abstract description 20
- 241000282898 Sus scrofa Species 0.000 title claims abstract description 20
- 239000000463 material Substances 0.000 title claims abstract description 19
- 230000001575 pathological effect Effects 0.000 claims abstract description 14
- 238000005520 cutting process Methods 0.000 claims description 43
- 238000003860 storage Methods 0.000 claims description 24
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 18
- 239000007853 buffer solution Substances 0.000 claims description 12
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 9
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 9
- 241001330002 Bambuseae Species 0.000 claims description 9
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 9
- 239000011425 bamboo Substances 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 9
- 229910052757 nitrogen Inorganic materials 0.000 claims description 9
- 241001233242 Lontra Species 0.000 claims description 4
- 238000005070 sampling Methods 0.000 abstract description 12
- 238000001514 detection method Methods 0.000 abstract description 4
- 241000700605 Viruses Species 0.000 abstract description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 8
- 201000010099 disease Diseases 0.000 description 6
- 241001465754 Metazoa Species 0.000 description 5
- 241000282887 Suidae Species 0.000 description 4
- 230000001154 acute effect Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 208000015181 infectious disease Diseases 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000007711 solidification Methods 0.000 description 3
- 230000008023 solidification Effects 0.000 description 3
- 206010011224 Cough Diseases 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 241000701386 African swine fever virus Species 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 206010011703 Cyanosis Diseases 0.000 description 1
- 208000000059 Dyspnea Diseases 0.000 description 1
- 206010013975 Dyspnoeas Diseases 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000002008 hemorrhagic effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 210000001165 lymph node Anatomy 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000004877 mucosa Anatomy 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
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- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
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- Biochemistry (AREA)
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- Genetics & Genomics (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a sample preparation device for suspected African swine fever pathological material detection in a pig farm, and particularly relates to the technical field of virus sampling equipment. The sampling tube is convenient to place and position, so that the samples are convenient to subpackage, multiple groups of samples can be prepared synchronously, the efficiency is improved, the whole structure is compact, the occupied space is small, and the use is convenient.
Description
Technical Field
The invention relates to the technical field of virus sampling equipment, in particular to a sample preparation device for detecting suspected African swine fever pathological material in a pig farm.
Background
African swine fever is an acute, hemorrhagic and virulent infectious disease caused by African swine fever virus infecting domestic pigs and various wild pigs (such as African wild pigs, European wild pigs, etc.). The world animal health Organization (OIE) classifies the animal epidemic disease as a legal report animal epidemic disease, and the disease is also a type of animal epidemic disease which is mainly prevented in China. The acute cough-relieving and acute infection has the characteristics of short disease process, high mortality rate of the most acute and acute infections up to 100%, fever (up to 40-42 ℃) as clinical manifestations, accelerated heartbeat, dyspnea, partial cough, serous or mucoid purulent secretion of eyes and nose, cyanosis of skin and obvious bleeding of lymph nodes, kidney and gastrointestinal mucosa. When the pig in a pig farm has the symptoms, the African swine fever cannot be directly diagnosed, a sample needs to be collected, a disease material is obtained after preparation, and then further detection is carried out.
In the preparation process of the sample, the tissue and organ with high animal metabolism and toxicity are generally adopted, small blocks are taken, a small amount of liquid nitrogen is put into the small blocks to enable the small blocks to be physically solidified at low temperature, then the small blocks are ground into powder, a centrifuge tube is filled with a proper amount of PBS buffer solution, the centrifuge tube is used for centrifuging the buffer solution, and supernatant is taken. However, the existing sample preparation device is time-consuming and labor-consuming independently in each step, so that the whole equipment is disordered and is not easy to overhaul, the occupied space is large, and the equipment is not easy to carry, so that the sample preparation device for detecting suspected African swine fever disease materials in a pig farm is provided.
Disclosure of Invention
The invention aims to provide a sample preparation device for detecting suspected African swine fever pathological materials in a pig farm, and aims to solve the problems that the conventional virus sample tissue preparation device in the background art is time-consuming and labor-consuming independently in each step, further causes overall equipment to be disordered and difficult to overhaul, occupies a large space, and causes equipment to be difficult to carry.
In order to achieve the purpose, the invention provides the following technical scheme: a sample preparation device for suspected African swine fever pathological material detection in a pig farm comprises a rack, wherein a cabinet door is hinged to the lower side of the front end of the rack, a fixing seat is fixed to the middle in the rack, a cutting barrel is rotatably connected to the upper side of the rack, the cutting barrel is communicated with the fixing seat, a rotating rod is rotatably connected to the cutting barrel, a cutting knife is fixed to the side face of the rotating rod, a first storage barrel is fixed to the upper side of the left fixing seat of the cutting barrel and communicated with the fixing seat through a first connecting pipe, a second storage barrel is fixed to the fixing seat on the right side of the cutting barrel, a baffle is slidably connected to the upper portion in the fixing seat, the rear portion of the baffle extends to the rear side of the rack, a positioning roller is fixed to the inner side of the fixing seat on the lower side of the baffle, the fixing base downside is fixed with the conical cover, the conical cover downside is fixed with the pipe, be fixed with the shunt tubes through the dead lever in the frame of pipe downside, shunt tubes downside symmetry is fixed with the honeycomb duct, the frame bottom is fixed with accomodates the groove, accomodate the groove upper end and have the support frame through the spring mounting, the support frame upside is provided with the locating plate, the locating plate is located the honeycomb duct downside.
Preferably, the equal symmetry of support frame upper left side and upper right side is fixed with the locating lever, the locating hole has all been seted up to locating plate upper end corner, the locating lever passes the locating hole, and has seted up the jack on the inboard locating plate of locating hole, the jack is located under the honeycomb duct and jack inner wall bonding is fixed with O type circle.
Preferably, the accommodating groove is connected with an accommodating box in a sliding mode, a classification plate is fixed in the accommodating box, a handle is fixed on the front side of the accommodating box, and the upper end of the accommodating groove and the lower end of the supporting frame are both fixed with magnetic blocks.
Preferably, four corners of frame downside all are fixed with the pulley, the frame upside is fixed with motor one, a motor downside output shaft and dwang fixed connection, frame left side middle part is fixed with the protection casing, the protection casing left side is fixed with motor two, two right side output shafts of motor and crushing roller fixed connection, crushing roller and crushing roller left side all are fixed with the sprocket, and connect through the chain between the sprocket, and the sprocket is located the protection casing.
Preferably, the upside is fixed with the feeder hopper before the frame, the feeder hopper rear side is linked together with a cutting section of thick bamboo, a cutting section of thick bamboo downside is fixed with the otter board, a cutting section of thick bamboo inner wall equidistance is fixed with supplementary cutter, supplementary cutter and cutting knife cross arrangement.
Preferably, the liquid nitrogen is stored in the first storage barrel, the PBS buffer solution is stored in the second storage barrel, a second connecting pipe is fixed on the lower side of the second storage barrel and connected with the conduit, and flow valves are installed on the first connecting pipe, the second connecting pipe and the conduit.
Preferably, a through pipe is fixed on each of the first storage barrel and the second storage barrel and extends to the left side and the right side of the rack.
Preferably, an electric push rod is fixed on the rear side of the rack, the rear end of the electric push rod is fixedly connected with the inner rear part of the baffle, and an electric cabinet is fixed on the front side of the rack.
Preferably, the fixing base is inboard to be seted up logical groove, and leads to the groove and extend to the frame rear side, baffle left side and right side all are fixed with the slider, and slider sliding connection leads to the inslot, the recess has been seted up to the bottom in the fixing base, crushing roller is located the recess, and the recess bottom has seted up the through-hole.
Preferably, the lower side is fixed with the box body through the fixed plate about the frame, the box body upside articulates there is the lid, the lower part is fixed with vibrating motor in the box body, the vibrating motor upside is fixed with places the board, it is fixed with the baffle in the box body of board upside to place, it is fixed with the rubber pad to place board and support frame inboard bonding.
Compared with the prior art, the invention has the beneficial effects that:
1. the sample is conveniently placed through the feed hopper, the sample is conveniently cut through the cutting cylinder, the rotating rod, the cutting knife and the auxiliary cutting knife, and the cut sample is conveniently discharged through the screen plate;
2. the cut samples are conveniently separated through the baffle, meanwhile, the cut small samples are conveniently solidified at low temperature through the first storage barrel and liquid nitrogen, the baffle is conveniently moved through the electric push rod, and the solidified samples fall to the bottom of the fixed seat to be conveniently crushed;
3. the crushing roller is conveniently driven to rotate through the motor II, the cooled sample is conveniently crushed through the matching of the crushing roller and the positioning roller, the crushed sample is conveniently crushed again through the matching of the crushing roller and the groove, the crushing effect is good, and the crushed sample is discharged through the through hole in the groove;
4. the crushed samples are conveniently collected through the conical cover, the split tubes and the guide pipes are conveniently separated, the sampling tubes are conveniently placed and positioned through the supporting frame, the positioning plate and the jacks, the samples are further conveniently subpackaged, multiple groups of samples can be synchronously prepared, and the efficiency is improved;
5. through storing PBS buffer solution in the bucket one, conveniently protect the sample, through vibrating motor, place board and baffle, conveniently vibrate the sampling tube, conveniently mix, and then conveniently follow-up centrifugal sampling that carries on, overall structure is compact, and occupation space is little, convenient to use.
Drawings
FIG. 1 is a block diagram of the present invention;
FIG. 2 is a front sectional view of the present invention;
FIG. 3 is a top cross-sectional view of a cutting drum and hopper of the present invention;
FIG. 4 is a side sectional view of a positioning roller, a crushing roller, and a crushing roller of the present invention;
FIG. 5 is a side view of the flapper and the power pushrod of the present invention;
FIG. 6 is a side sectional view of the cartridge body of the present invention;
FIG. 7 is a view showing the structure of the positioning plate and the insertion hole in the present invention;
FIG. 8 is a view showing the construction of the stand according to the present invention;
FIG. 9 is a top view of the receiver and separator plate of the present invention;
FIG. 10 is an enlarged view of FIG. 2 at A.
In the figure: 1. a first motor; 2. a frame; 3. an electric cabinet; 4. a protective cover; 5. a second motor; 6. a cabinet door; 7. a pulley; 8. a second connecting pipe; 9. a feed hopper; 10. cutting the cylinder; 11. a first storage barrel; 12. a first connecting pipe; 13. a conduit; 14. a box body; 15. positioning a plate; 16. a spring; 17. a receiving groove; 18. a storage box; 19. a jack; 20. a support frame; 21. positioning a rod; 22. a flow guide pipe; 23. a shunt tube; 24. a conical cover; 25. a crushing roller; 26. a crushing roller; 27. a baffle plate; 28. a fixed seat; 29. a second storage barrel; 30. an auxiliary cutter; 31. a cutting knife; 32. rotating the rod; 33. a positioning roller; 34. an electric push rod; 35. placing the plate; 36. a vibration motor; 37. a partition plate; 38. a classification plate; 39. a slider; 40. a groove; 41. a mesh plate.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-10, the present invention provides a technical solution: a sample preparation device for suspected African swine fever disease material detection in a pig farm comprises a frame 2, a cabinet door 6 is hinged to the lower side of the front end of the frame 2, a fixing seat 28 is fixed to the middle in the frame 2, a cutting barrel 10 is rotatably connected to the frame 2 on the upper side of the fixing seat 28, the cutting barrel 10 is communicated with the fixing seat 28, a rotating rod 32 is rotatably connected to the cutting barrel 10, a cutting knife 31 is fixed to the side face of the rotating rod 32, a first storage barrel 11 is fixed to the upper side of the fixing seat 28 on the left side of the cutting barrel 10, the first storage barrel 11 is communicated with the fixing seat 28 through a first connecting pipe 12, a second storage barrel 29 is fixed to the fixing seat 28 on the right side of the cutting barrel 10, a baffle plate 27 is slidably connected to the upper portion in the fixing seat 28, the rear portion of the baffle plate 27 extends to the rear side of the frame 2, a positioning roller, the utility model discloses a fixed seat 28 downside is fixed with conical cover 24, and 24 downside of conical cover are fixed with pipe 13, are fixed with shunt tubes 23 through the dead lever in the frame 2 of pipe 13 downside, and shunt tubes 23 downside symmetry is fixed with honeycomb duct 22, and frame 2 bottom is fixed with accomodates groove 17, accomodates the groove 17 upper end and is fixed with support frame 20 through spring 16, and support frame 20 upside is provided with locating plate 15, and locating plate 15 is located the honeycomb duct 22 downside.
In the invention: 20 upper left sides of support frame and the equal symmetry of upper right side are fixed with locating lever 21, the locating hole has all been seted up to 15 upper end corners of locating plate, locating lever 21 passes the locating hole, and seted up jack 19 on the inboard locating plate 15 of locating hole, jack 19 is located under the honeycomb duct 22 and the 19 inner walls bonding of jack are fixed with O type circle, through locating lever 21 and locating hole, conveniently support and spacing locating plate 15, conveniently take, through jack 19 and O type circle, conveniently carry on spacingly to the sampling tube, conveniently carry out the partial shipment.
In the invention: accomodate groove 17 sliding connection and have receiver 18, receiver 18 internal fixation has categorised board 38, and receiver 18 front side is fixed with the handle, accomodates groove 17 upper end and 20 lower extremes of support frame and all is fixed with the magnetic path, through receiver 18 and categorised board 38, conveniently classifies article and deposits, through the handle, makes things convenient for personnel to control, through the magnetic path, conveniently fixes a position support frame 20.
In the invention: four corners of 2 downside of frame all are fixed with pulley 7, 2 upsides of frame are fixed with motor 1, 1 downside output shaft of motor and dwang 32 fixed connection, 2 left side middle parts of frame are fixed with protection casing 4, protection casing 4 left sides are fixed with motor two 5, two 5 right side output shafts of motor and crushing roller 26 fixed connection, crushing roller 26 and crushing roller 25 left sides all are fixed with the sprocket, and connect through the chain between the sprocket, the sprocket is located protection casing 4, through pulley 7, conveniently remove, through motor 1, conveniently drive dwang 32 and rotate, through motor two 5, conveniently drive crushing roller 26, through sprocket and chain, it rotates simultaneously to drive crushing roller 25 when convenient crushing roller 26 rotates, and pass through protection casing 4, conveniently protect sprocket and chain.
In the invention: upside is fixed with feeder hopper 9 before frame 2, and the 9 rear sides of feeder hopper are linked together with a cutting section of thick bamboo 10, and a cutting section of thick bamboo 10 downside is fixed with otter board 41, and a cutting section of thick bamboo 10 inner wall equidistance is fixed with supplementary cutter 30, and supplementary cutter 30 and cutting knife 31 cross arrangement through feeder hopper 9, conveniently place the sample, through otter board 41, conveniently support and filter the sample, through supplementary cutter 30, improve the cutting effect to the sample.
In the invention: store and to have the liquid nitrogen in the bucket 11, store and to have PBS buffer solution in the bucket two 29, store two 29 downside of bucket and be fixed with two 8 of connecting pipe, two 8 of connecting pipe are connected with pipe 13, connecting pipe one 12, all install the flow valve on two 8 of connecting pipe and the pipe 13, through the liquid nitrogen, conveniently carry out low temperature to the sample and solidify, conveniently smash, through PBS buffer solution, conveniently protect the sample, through connecting pipe two 8, conveniently add PBS buffer solution in to fixing base 28, through the flow valve, conveniently control the flow.
In the invention: all be fixed with the siphunculus on storage bucket 11 and the storage bucket two 29, and the siphunculus extends to frame 2 left side and right side, through the siphunculus, conveniently adds liquid nitrogen and PBS buffer solution.
In the invention: 2 rear sides of frame are fixed with electric putter 34, and rear portion fixed connection in electric putter 34 rear sides stretches end and baffle 27, and 2 front sides of frame are fixed with electric cabinet 3, through electric putter 34, drive baffle 27 and remove, and the sample after conveniently making the solidification falls down, conveniently smashes, through electric cabinet 3, conveniently controls equipment.
In the invention: through groove has been seted up to fixing base 28 inboard, and lead to the groove and extend to 2 rear sides in the frame, baffle 27 left side and right side all are fixed with slider 39, and slider 39 sliding connection is in leading to the inslot, recess 40 has been seted up to the bottom in fixing base 28, crushing roller 25 is located recess 40, and the through-hole has been seted up to recess 40 bottom, through leading to groove and slider 39, make things convenient for baffle 27 to remove, through recess 40, conveniently cooperate with crushing roller 25, smash once more after the breakage, guarantee crushing effect.
In the invention: frame 2 left side downside is fixed with box body 14 through the fixed plate, and 14 upsides of box body articulate has the lid, and the lower part is fixed with vibrating motor 36 in the box body 14, and the 36 upsides of vibrating motor are fixed with places board 35, and the 14 internal fixations of box body of placing board 35 upside have baffle 37, place board 35 and the inboard bonding of support frame 20 and be fixed with the rubber pad, conveniently vibrate the mix to the sampling tube after the partial shipment, convenient follow-up centrifugation.
The working principle is as follows: when in normal use, the device is powered on, then the cabinet door 6 is opened, the support frame 20 is pushed downwards to extrude the spring 16 until the magnetic block is attracted, the support frame 20 is fixed, then the sampling tube is inserted into the jack 19 to be placed, then the support frame 20 is reset, then the sample is placed in the cutting cylinder 10 through the feed hopper 9, then the motor 1 is operated, the motor 1 drives the cutting knife 31 to rotate, and simultaneously the sample is cut through the matching of the motor 1 and the auxiliary cutter 30, and then the sample falls on the upper side of the baffle 27 through the screen plate 41, at the moment, liquid nitrogen is introduced to carry out low-temperature solidification on the small sample, then the electric push rod 34 is operated, the electric push rod 34 drives the baffle 27 to move backwards, so that the solidified sample falls, at the moment, the motor two 5 is operated, the crushing roller 26 is driven to rotate by the motor two 5, and the crushing roller 25 is driven to rotate through the chain wheel and the, through the cooperation of crushing roller 26 and registration roller 33, the realization is broken the sample, fall into recess 40 until the sample after the breakage, and then smash once more through the cooperation with crushing roller 25, until passing the through-hole, fall into conical cover 24, and through shunt tubes 23 and pipe 13 with the sample partial shipment after smashing to the sample tube, then open the flow valve on two 8 connecting pipes, make the PBS buffer solution in two 29 storage barrels get into in the pipe 13, then get into in the sample tube respectively, then can take out the sample tube and seal, and place in box body 14, through vibrating motor 36, vibrate, make sample and PBS buffer solution mixing, can centrifuge through centrifuge and centrifugate, it can to get its supernatant, realize the preparation.
In summary, the following steps: the invention is convenient for placing samples through the feed hopper 9, is convenient for cutting the samples through the cutting cylinder 10, the rotating rod 32, the cutting and auxiliary cutter 30, is convenient for discharging the cut samples through the screen plate 41, is convenient for separating the cut samples through the baffle plate 27, is convenient for low-temperature solidification of small cut samples through the storage barrel I11 and liquid nitrogen, is convenient for moving the baffle plate 27 through the electric push rod 34, further falls the solidified samples at the bottom of the fixed seat 28, is convenient for crushing, is convenient for driving the crushing roller 26 to rotate through the motor II 5, further is convenient for crushing the cooled samples through the matching of the crushing roller 26 and the positioning roller 33, is convenient for re-crushing the crushed samples through the matching of the crushing roller 25 and the groove 40, and ensures good crushing effect, and discharge through the through-hole in the recess 40, through conical cover 24, the convenience is collected the sample after smashing, conveniently separate through shunt tubes 23 and pipe 13, through support frame 20, locating plate 15 and jack 19, conveniently place and fix a position the sampling tube, and then conveniently carry out the partial shipment to the sample, can prepare multiunit sample in step, the efficiency is improved, through the PBS buffer solution in storage bucket 11, conveniently protect the sample, through vibrating motor 36, place board 35 and baffle 37, conveniently vibrate the sampling tube, conveniently mix, and then conveniently follow-up centrifugal sampling that carries out, overall structure is compact, small space occupation, high durability and convenient use.
The points to be finally explained are: first, in the description of the present application, it should be noted that, unless otherwise specified and limited, the terms "mounted," "connected," and "connected" should be understood broadly, and may be a mechanical connection or an electrical connection, or a communication between two elements, and may be a direct connection, and "upper," "lower," "left," and "right" are only used to indicate a relative positional relationship, and when the absolute position of the object to be described is changed, the relative positional relationship may be changed;
secondly, the method comprises the following steps: in the drawings of the disclosed embodiments of the invention, only the structures related to the disclosed embodiments are referred to, other structures can refer to common designs, and the same embodiment and different embodiments of the invention can be combined with each other without conflict;
and finally: the present invention is not limited to the above preferred embodiments, but rather, any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A sample preparation device for detecting suspected African swine fever pathological material in a pig farm comprises a frame (2), and is characterized in that: the utility model discloses a cutting machine, including frame (2), cutting barrel (10) and fixing base (28), frame (2) internal rotation of fixing base (28) upside has a cabinet door (6) to articulate, middle part is fixed with fixing base (28) in the frame (2), the frame (2) internal rotation of fixing base (28) upside is connected with cutting barrel (10), cutting barrel (10) internal rotation is connected with dwang (32), dwang (32) side is fixed with cutting knife (31), the left fixing base (28) upside of cutting barrel (10) is fixed with storage bucket (11), storage bucket (11) are linked together with fixing base (28) through connecting pipe (12), be fixed with storage bucket two (29) on fixing base (28) on cutting barrel (10) right side, upper portion sliding connection has baffle (27) in fixing base (28), baffle (27) rear portion extends to frame (2) rear side, a positioning roller (33) is fixed in the fixed seat (28) at the lower side of the baffle plate (27), a crushing roller (26) is rotationally connected in a fixed seat (28) at the inner side of the positioning roller (33), a crushing roller (25) is rotationally connected in a fixed seat (28) at the lower side of the crushing roller (26), a conical cover (24) is fixed on the lower side of the fixed seat (28), a guide pipe (13) is fixed on the lower side of the conical cover (24), a shunt tube (23) is fixed in the frame (2) at the lower side of the conduit (13) through a fixing rod, flow guide pipes (22) are symmetrically fixed on the lower side of the flow distribution pipe (23), a containing groove (17) is fixed at the bottom of the frame (2), a supporting frame (20) is fixed at the upper end of the accommodating groove (17) through a spring (16), the locating plate (15) is arranged on the upper side of the support frame (20), and the locating plate (15) is located on the lower side of the guide pipe (22).
2. The sample preparation device for detecting suspected African swine fever pathological material in a pig farm according to claim 1, wherein: the utility model discloses a honeycomb duct is fixed with O type circle, locating lever (21) have been all fixed with to the equal symmetry of support frame (20) upper left side and upper right side, locating plate (15) upper end corner has all seted up the locating hole, locating lever (21) pass the locating hole, and seted up jack (19) on locating plate (15) of locating hole inboard, jack (19) are located positive downside of honeycomb duct (22) and jack (19) inner wall bonding is fixed with O type circle.
3. The sample preparation device for detecting suspected African swine fever pathological material in a pig farm according to claim 1, wherein: accomodate groove (17) sliding connection has receiver (18), receiver (18) internal fixation has categorised board (38), receiver (18) front side is fixed with the handle, accomodate groove (17) upper end and support frame (20) lower extreme and all be fixed with the magnetic path.
4. The sample preparation device for detecting suspected African swine fever pathological material in a pig farm according to claim 1, wherein: four corners of frame (2) downside all are fixed with pulley (7), frame (2) upside is fixed with motor (1), motor (1) downside output shaft and dwang (32) fixed connection, frame (2) left side middle part is fixed with protection casing (4), protection casing (4) left side is fixed with motor two (5), motor two (5) right side output shaft and crushing roller (26) fixed connection, crushing roller (26) and crushing roller (25) left side all are fixed with the sprocket, and connect through the chain between the sprocket, and the sprocket is located protection casing (4).
5. The sample preparation device for detecting suspected African swine fever pathological material in a pig farm according to claim 1, wherein: the upper side is fixed with feeder hopper (9) before frame (2), feeder hopper (9) rear side is linked together with a cutting section of thick bamboo (10), a cutting section of thick bamboo (10) downside is fixed with otter board (41), a cutting section of thick bamboo (10) inner wall equidistance is fixed with supplementary cutter (30), supplementary cutter (30) and cutting knife (31) cross arrangement.
6. The sample preparation device for detecting suspected African swine fever pathological material in a pig farm according to claim 1, wherein: store and be stored with the liquid nitrogen in bucket (11), store and have PBS buffer solution in bucket two (29), store bucket two (29) downside and be fixed with connecting pipe two (8), connecting pipe two (8) are connected with pipe (13), all install the flow valve on connecting pipe one (12), connecting pipe two (8) and pipe (13).
7. The sample preparation device for detecting suspected African swine fever pathological material in a pig farm according to claim 1, wherein: and the first storage barrel (11) and the second storage barrel (29) are respectively fixed with a through pipe, and the through pipes extend to the left side and the right side of the rack (2).
8. The sample preparation device for detecting suspected African swine fever pathological material in a pig farm according to claim 1, wherein: an electric push rod (34) is fixed on the rear side of the rack (2), the rear end of the electric push rod (34) is fixedly connected with the inner rear part of the baffle plate (27), and an electric cabinet (3) is fixed on the front side of the rack (2).
9. The sample preparation device for detecting suspected African swine fever pathological material in a pig farm according to claim 1, wherein: through groove has been seted up to fixing base (28) inboard, and lead to the groove and extend to frame (2) rear side, baffle (27) left side and right side all are fixed with slider (39), and slider (39) sliding connection lead to the inslot, recess (40) have been seted up to the bottom in fixing base (28), crushing roller (25) are located recess (40), and the through-hole has been seted up to recess (40) bottom.
10. The sample preparation device for detecting suspected African swine fever pathological material in a pig farm according to claim 1, wherein: frame (2) left side downside is fixed with box body (14) through the fixed plate, box body (14) upside articulates there is the lid, lower part is fixed with vibrating motor (36) in box body (14), vibrating motor (36) upside is fixed with places board (35), place box body (14) internal fixation of board (35) upside and have baffle (37), place board (35) and support frame (20) inboard bonding fixation have the rubber pad.
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Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN205357208U (en) * | 2016-03-10 | 2016-07-06 | 黄秀淼 | Poultry fodder reducing mechanism |
| WO2018018888A1 (en) * | 2016-07-26 | 2018-02-01 | 张建国 | Automatic processing apparatus for waste slurry bag |
| CN108772982A (en) * | 2018-06-08 | 2018-11-09 | 国宏中晶（北京）科技发展有限公司 | A kind of waste tire fine glue powder preparation facilities that granularity is controllable and method |
| CN108903037A (en) * | 2018-06-13 | 2018-11-30 | 淮北普成饲料有限公司 | A kind of raw material grinding device and its application method for feed processing |
| CN109813904A (en) * | 2019-03-27 | 2019-05-28 | 丁建红 | African swine fever virus rapid detection card |
| CN110241258A (en) * | 2019-06-19 | 2019-09-17 | 湖南新南方养殖服务有限公司 | The method whether detection pig farm birds or its living environment carry African swine fever virus |
| CN210071437U (en) * | 2019-06-25 | 2020-02-14 | 宁波升一检测技术服务有限公司 | Sample collection device for leather formaldehyde test |
| US20200070207A1 (en) * | 2019-05-06 | 2020-03-05 | Xianrong Wang | Component Box for Optimizing Screening and Taking Work |
| CN111036323A (en) * | 2018-10-11 | 2020-04-21 | 陕西前进齿科新技术开发有限公司 | Tooth detects with tooth reducing mechanism of being convenient for collect material |
| JP6696073B1 (en) * | 2019-12-04 | 2020-05-20 | 中国計量大学 | Reliable Authenticity Identification Method for Saiko Tatsui Tea |
| CN210583848U (en) * | 2019-09-24 | 2020-05-22 | 兴化市宇工机械有限公司 | Fruit vegetables food safety monitoring is with smashing filtrating separator |
-
2020
- 2020-06-02 CN CN202010490803.5A patent/CN111454826A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN205357208U (en) * | 2016-03-10 | 2016-07-06 | 黄秀淼 | Poultry fodder reducing mechanism |
| WO2018018888A1 (en) * | 2016-07-26 | 2018-02-01 | 张建国 | Automatic processing apparatus for waste slurry bag |
| CN108772982A (en) * | 2018-06-08 | 2018-11-09 | 国宏中晶（北京）科技发展有限公司 | A kind of waste tire fine glue powder preparation facilities that granularity is controllable and method |
| CN108903037A (en) * | 2018-06-13 | 2018-11-30 | 淮北普成饲料有限公司 | A kind of raw material grinding device and its application method for feed processing |
| CN111036323A (en) * | 2018-10-11 | 2020-04-21 | 陕西前进齿科新技术开发有限公司 | Tooth detects with tooth reducing mechanism of being convenient for collect material |
| CN109813904A (en) * | 2019-03-27 | 2019-05-28 | 丁建红 | African swine fever virus rapid detection card |
| US20200070207A1 (en) * | 2019-05-06 | 2020-03-05 | Xianrong Wang | Component Box for Optimizing Screening and Taking Work |
| CN110241258A (en) * | 2019-06-19 | 2019-09-17 | 湖南新南方养殖服务有限公司 | The method whether detection pig farm birds or its living environment carry African swine fever virus |
| CN210071437U (en) * | 2019-06-25 | 2020-02-14 | 宁波升一检测技术服务有限公司 | Sample collection device for leather formaldehyde test |
| CN210583848U (en) * | 2019-09-24 | 2020-05-22 | 兴化市宇工机械有限公司 | Fruit vegetables food safety monitoring is with smashing filtrating separator |
| JP6696073B1 (en) * | 2019-12-04 | 2020-05-20 | 中国計量大学 | Reliable Authenticity Identification Method for Saiko Tatsui Tea |
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