CN110241258A - The method whether detection pig farm birds or its living environment carry African swine fever virus - Google Patents
The method whether detection pig farm birds or its living environment carry African swine fever virus Download PDFInfo
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- CN110241258A CN110241258A CN201910534706.9A CN201910534706A CN110241258A CN 110241258 A CN110241258 A CN 110241258A CN 201910534706 A CN201910534706 A CN 201910534706A CN 110241258 A CN110241258 A CN 110241258A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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Abstract
The invention discloses a kind of methods whether detection pig farm birds or its living environment carry African swine fever virus comprising following steps: Step 1: searching the bird's nest in pig farm and within the scope of 500 meters of pig farm periphery, bird's nest, then enter step two if it does not exist；Bird's nest if it exists wipes bird's nest surface area with cotton swab, and detection bird's nest whether there is African swine fever virus；If the testing result of all bird's nests is feminine gender, it can determine that birds or birdife environment do not carry African swine fever virus in pig farm；If there is positive presence in the testing result of all bird's nests, it can determine that birds or birdife environment carry African swine fever virus in pig farm；Step 2: getting out the healthy pigeon that male and female is pairs of, does not catch an illness, difference number is carried out by the difference of male and female in the upper mounting and positioning device of every pigeon, and by pigeon, pigeon is carried out and lets test fly away.The present invention accurately and rapidly can detect or verify whether birds or birdife environment in pig farm carry African swine fever virus, in order to provide foundation if appropriate for reproducing for pig farm.
Description
Technical field
The invention belongs to swine fever detections, Prevention Technique field, and in particular to a kind of detection pig farm birds or its living environment
The method for whether carrying African swine fever virus.
Background technique
African swine fever is one of a kind of animal epidemics of China's guard key, it is a kind of acute, hemorrhagic, strong pig
Infectious disease, clinical manifestation are fever, and body temperature is increased up to 40~42 DEG C, and palpitating speed, expiratory dyspnea is partially coughed, and eye, nose have slurry
Fluidity or mucus purulent secretion, skin cyanosis, the obvious bleeding of lymph node, kidney, gastrointestinal mucosa, symptom occur only that 1~2d is just
Death, without exact morbidity season.It is seriously congested that the heart, liver, spleen, lymph occurs in autopsy pig, shaped like hemotoncus, then suspicious
For African swine fever.It finally makes a definite diagnosis and needs by laboratory using hemadsorption test, direct immuno fluorescence test, animal inoculation pvaccination
Test, immunoelectrophoretic test, enzyme-linked immunosorbent assay etc..
African swine fever is classified as statutory report animal epidemic by World Organization for Animal Health (OIE), which is also China's emphasis
A kind of animal epidemics of prevention.The vaccine and needleless of African swine fever can effectively be prevented due to not developing worldwide
Property drug can be cured, thus fast and accurately diagnose and prevent and treat to prevent the disease propagation, prevalence it is particularly significant.Currently, non-
Although continent swine fever, in the non-large-scale outbreak in China, only in fashion trend is distributed, the number of animals raised on China pig farm is huge, many pig farms
Full group of pigs is only slaughtered after infection African swine fever virus, so direct economic loss caused by African swine fever is still more tight
Weight.Relative to common swine fever virus, due to African swine fever virus at room temperature can viable for weeks, and currently without can have
There is Africa in the vaccine of effect prevention African swine fever, therefore the pig farm for infecting African swine fever, pig farm or pig farm ambient enviroment
The risk of swine fever virus is high.In order to effectively and reasonably using idle pig farm, after African swine fever virus is infected on some pig farms, pass through
The cleaning of a period of time, cleaning, disinfection, it is vacant after can introduce a fine variety and reproduce once again, but reproduce and how to detect or verify before pig farm
It inside whether there is or carry African swine fever virus, be a great problem of pig-breeding industry.
The bio-security on pig farm, which refers to, is passed to swinery simultaneously for infectious disease and pollutant for prevention wild animal and insect
The effective prevention and control measure for preventing propagation and taking, is the key means of prevention and control African swine fever.In general, mosquito of the people to pig farm
Fly, the precautionary measures of mouse are fairly proper, but ignore the prevention and control of birds.Birds can not only steal the diet for eating pig farm, cause pig
Feeding day flow vector reduction, decline the weight gain of pig；In addition birds may infect some diseases to the pig in pig farm, common
Have colibacillosis of pigs, swine dysentery and porcine salmonellosis etc., and African swine fever virus also can be propagated and carry to birds.It is right
In the pig farm for breaking out African swine fever, birds are possible to carry the life that African swine fever virus makes virus spread and remain in birds
In environment.When preparing to reproduce after the disinfection of pig farm, remaining African swine fever in birdife environment is carried or is present in by birds
Virus can form potential threat to pig farm, will increase the risk that healthy live pig infects African swine fever virus again, therefore in pig
When reproducing or creating pig farm addressing, need to detect or verify birds or birdife environment in pig farm whether carry it is non-
Continent swine fever virus, to reduce the risk that pig farm is caught an illness again.In the prior art, although disclosing a large amount of detection African swine fever viruses
Method, such as Pathogen test, Nucleic Acid Identification and serological test, but these methods be required to provide specific test sample or
Detected object, such as detected using the pathological material of disease of pig, Nasopharyngeal swabs, blood as sample, and for birds or birdife ring
The detection of African swine fever virus in border, can not but obtain tested sample or object is used to identify whether African swine fever virus deposits
?.
Summary of the invention
In view of the above-mentioned problems, the present invention provides a kind of detection pig farm birds or its living environment whether to carry African swine fever
The method of virus, accurately and rapidly can detect or verify whether birds or birdife environment in pig farm carry African pig
Pestivirus, in order to provide foundation if appropriate for reproducing for pig farm.
The present invention is achieved through the following technical solutions.
The method whether detection pig farm birds or its living environment carry African swine fever virus, it is characterised in that including as follows
Step:
Step 1: searching the bird's nest in pig farm and within the scope of 500 meters of pig farm periphery, bird's nest, then entered step if it does not exist
Two, to detect whether that the long-distance birds migrated carry virus to pig farm；Bird's nest if it exists is taken with cotton swab wiping bird's nest surface
Sample, detection bird's nest whether there is African swine fever virus；If the testing result of all bird's nests is feminine gender, it can determine that birds in pig farm
Or birdife environment does not carry African swine fever virus；If there is positive presence in the testing result of all bird's nests, pig can determine that
Birds or birdife environment carry African swine fever virus in；
Step 2: the healthy pigeon that male and female is pairs of, does not catch an illness is got out, in the upper mounting and positioning device of every pigeon, and will
Pigeon carries out difference number by the difference of male and female；In the stage of selection 3~5 days lasting fine days, each pair of pigeon is placed in pig farm and is put in a suitable place to breed；
Each pair of pigeon is first placed in cage and raises 1~2 day by period, and pigeon is made to adapt to pig farm ambient enviroment；Later 3~4 days, daily
All public dove or hen pigeon are let fly away from cage, move freely pigeon outside cage and looks for food；Daily after pigeon is flown back, with speckling with life
The cotton swab wiping pigeon whole body sampling for managing salt water, successively detects the sampling for pigeon of flying back, detects whether there is Africa
Swine fever virus；If there is positive test symbol appearance, all pigeons are let fly away after stopping, and can determine whether birds or bird in pig farm
Class living environment carries African swine fever virus；If all testing results are feminine gender, it can determine whether birds or birds in pig farm
Living environment does not carry African swine fever virus.
As optimal technical scheme, in the step 2, the pairs of pigeon of male and female in pig farm put in a suitable place to breed quantity be 20~
100.
As optimal technical scheme, in the step 2, the time that pigeon is let fly away daily is no less than 4h.
As optimal technical scheme, in the step 2, after pigeon is let fly away, by positioning device monitor pigeon movement away from
From and scope of activities, wherein the smallest 1~3 pigeon of moving distance or not in pig farm movable pigeon not as African swine fever
The sampling object of viral diagnosis.
It further include that the pigeon of flying back that sample detection is the positive disappears in the step 2 as optimal technical scheme
The step of poison.
It is described Step 1: African swine fever virus uses PCR fluorescence detection in step 2 as optimal technical scheme.
The invention has the advantages that:
1) the method for the present invention can accurate detection identify in pig farm birds or birdife environment with the presence or absence of hog cholera
Poison leads to healthy live pig so as to provide pig farm if appropriate for the foundation reproduced for raiser to reduce reproducing for pig farm blindness
The economic loss of the possibility and raiser fallen ill again.
2) the method for the present invention is simple, scientific, has preferable practical value, can be in large-scale application and broke out in judgement
With the presence or absence of virus in the pig farm birds zone of action of African swine fever, and targetedly disinfectant measure is taken, to effectively prevent leading to
The activity for crossing birds makes African swine fever virus long range diffusion and propagation.
Specific embodiment
The technical solution in the present invention is clearly and completely described below, it is clear that described embodiment is only
Several embodiments therein of the invention, instead of all the embodiments.Based on the embodiments of the present invention, the common skill in this field
Art personnel every other embodiment obtained without creative efforts belongs to the model that the present invention protects
It encloses.
Embodiment 1
Certain the morbidity pig house for selecting agriculture rural area portion to announce as detection place, all slaughtered by the pig on the pig farm, and
And cleaning, cleaning, disinfection are carried out；It after lifting a blockade 4 months, is intended to prepare to introduce a fine variety again to reproduce, now be examined using the method for the present invention
It surveys pig farm birds or whether its living environment carries African swine fever virus, to be confirmed whether that there are risks, the specific steps are as follows: search
The bird's nest within the scope of 500 meters of in pig farm and pig farm periphery is sought, finds bird's nest 3 altogether；Bird's nest surface area is wiped with cotton swab,
African swine fever virus whether there is using PCR fluorescence detection, concrete outcome is shown in Table 1；
The African swine fever virus testing result of 1 bird's nest of table
| Bird's nest number | Testing result |
| 1 | 37.18 |
| 2 | NoCt |
| 3 | NoCt |
| 4 | NoCt |
| 5 | 36.82 |
| Negative control | NoCt |
| Positive control | 22.20 |
As shown in Table 1, in the African swine fever virus testing result of bird's nest, the test positive of No. 1 and No. 5 bird's nest then may be used
Determine that birds or birdife environment carry African swine fever virus in pig farm, pig farm is reproduced at this time occurs again there are epidemic situation
Risk.
Embodiment 2
Certain the morbidity pig house for selecting agriculture rural area portion to announce as place to be detected, all slaughtered by the pig on the pig farm,
And cleaning, cleaning, disinfection are carried out；It lifts a blockade after a period of time, is intended to prepare to introduce a fine variety again to reproduce, now use present invention side
Whether method detection pig farm birds or its living environment carry African swine fever virus, and to be confirmed whether that there are risks, specific steps are such as
Under: the bird's nest in pig farm and within the scope of 500 meters of pig farm periphery is searched, finds bird's nest 2 altogether；Bird's nest surface is wiped with cotton swab
Sampling whether there is African swine fever virus using PCR fluorescence detection, and concrete outcome is shown in Table 2；
The African swine fever virus testing result of 2 bird's nest of table
| Bird's nest number | Testing result |
| 1 | NoCt |
| 2 | NoCt |
| Negative control | NoCt |
| Positive control | 21.20 |
As shown in Table 2, in the African swine fever virus testing result of bird's nest, No. 1 and No. 2 bird's nest is detected as feminine gender, then may be used
Determine that birds or birdife environment do not carry African swine fever virus in pig farm.
Embodiment 3
As place to be detected, the pig on the pig farm is all slaughtered on certain the morbidity pig farm for selecting agriculture rural area portion to announce,
And cleaning, cleaning, disinfection are carried out；It lifts a blockade and after vacant a period of time, is intended to prepare to introduce a fine variety again to reproduce, now using this
Whether inventive method detection pig farm birds or its living environment carry African swine fever virus, to be confirmed whether that there are risks, specifically
Steps are as follows:
Step 1: searching the bird's nest in pig farm and within the scope of 500 meters of pig farm periphery, bird's nest is not found, then enters step two,
To detect whether that the long-distance birds migrated carry virus to pig farm；
Step 2: be ready to male and female in pairs, do not catch an illness healthy pigeon 50,25 pairs altogether, installed on every pigeon
Positioning device, and pigeon is subjected to difference number by the difference of male and female；The stage of selection 3~5 days lasting fine days, by each pair of pigeon
Pig farm is placed in put in a suitable place to breed；Each pair of pigeon is first placed in cage and raises 1 day by period, and pigeon is made to adapt to pig farm ambient enviroment；Later 4
It, all public dove or hen pigeon are let fly away, move freely pigeon outside cage and look for food from cage daily, the time that pigeon is let fly away daily
No less than 4h, and monitor by positioning device the moving distance and scope of activities of pigeon, wherein the smallest 2 pigeons of moving distance
Or the sampling object that movable pigeon detects not as African swine fever virus not in pig farm；Daily after pigeon is flown back, with being stained with
There is the cotton swab wiping pigeon whole body sampling of physiological saline, detects whether to carry African swine fever virus, detection knot using fluorescent PCR
Fruit is shown in Table 3；
As shown in Table 3, pigeon was let fly away to the 2nd day, and the pigeon of number 2-2,4-2,18-2 detect positive findings, therefore can
Birds or birdife environment are determined in pig farm there are African swine fever virus, pig farm reproduces that there are the wind that epidemic situation occurs again at this time
Danger.
The African swine fever virus testing result of 3 pigeon of table
Claims (6)
1. the method whether detection pig farm birds or its living environment carry African swine fever virus, it is characterised in that including walking as follows
It is rapid:
Step 1: searching the bird's nest in pig farm and within the scope of 500 meters of pig farm periphery, bird's nest, then enter step two if it does not exist；If
There are bird's nests, wipe bird's nest surface area with cotton swab, detection bird's nest whether there is African swine fever virus；If the inspection of all bird's nests
Surveying result is feminine gender, can determine that birds or birdife environment do not carry African swine fever virus in pig farm；If all bird's nests
There is positive presence in testing result, then can determine that birds or birdife environment carry African swine fever virus in pig farm；
Step 2: be ready to male and female in pairs, the healthy pigeon that does not catch an illness, in the upper mounting and positioning device of every pigeon, and by pigeon
Difference number is carried out by the difference of male and female；In the stage of selection 3~5 days lasting fine days, each pair of pigeon is placed in pig farm and is put in a suitable place to breed；Phase
Between, first each pair of pigeon is placed in cage and is raised 1~2 day, pigeon is made to adapt to pig farm ambient enviroment；Later 3~4 days, daily will
All public affairs dove or hen pigeon are let fly away from cage, move freely pigeon outside cage and look for food；Daily after pigeon is flown back, with speckling with physiology
The cotton swab wiping pigeon whole body sampling of salt water, successively detects the sampling for pigeon of flying back, detects whether there is African pig
Pestivirus；If there is positive test symbol appearance, all pigeons are let fly away after stopping, and can determine whether birds or birds in pig farm
Living environment carries African swine fever virus；If all testing results are feminine gender, it can determine whether that birds or birds are raw in pig farm
Environment living does not carry African swine fever virus.
2. the method whether detection pig farm birds or its living environment carry African swine fever virus as described in claim 1,
It is characterized in that in the step 2, the quantity of putting in a suitable place to breed of the pairs of pigeon of the male and female in pig farm is 20~100.
3. the method whether detection pig farm birds or its living environment carry African swine fever virus as described in claim 1,
It is characterized in that in the step 2, the time that pigeon is let fly away daily is no less than 4h.
4. the method whether detection pig farm birds or its living environment carry African swine fever virus as described in claim 1,
It is characterized in that in the step 2, after pigeon is let fly away, the moving distance and scope of activities of pigeon is monitored by positioning device, wherein
The smallest 1~3 pigeon of moving distance or the sampling pair that movable pigeon detects not as African swine fever virus not in pig farm
As.
5. the method whether detection pig farm birds or its living environment carry African swine fever virus as described in claim 1,
It is characterized in that in the step 2 further including the steps that being that positive pigeon of flying back carries out disinfection by sample detection.
6. such as the detection pig farm birds as described in Claims 1 to 5 is any or whether its living environment carry African swine fever virus
Method, it is characterised in that described Step 1: African swine fever virus uses PCR fluorescence detection in step 2.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112359144A (en) * | 2020-11-24 | 2021-02-12 | 广西农垦永新畜牧集团有限公司良圻原种猪场 | Method for monitoring and eliminating African swine fever virus in large-scale pig farm |
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2019
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| CN109295255A (en) * | 2018-09-18 | 2019-02-01 | 张薇 | A kind of nucleic acid rapid detection method for African swine fever virus |
| CN109593893A (en) * | 2019-02-03 | 2019-04-09 | 郑州中道生物技术有限公司 | African hog cholera virus fluorescent PCR quick detection kit |
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| CN112359144A (en) * | 2020-11-24 | 2021-02-12 | 广西农垦永新畜牧集团有限公司良圻原种猪场 | Method for monitoring and eliminating African swine fever virus in large-scale pig farm |
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Address after: 410600 Room 401, No.001 Jinzhou North Road, Ningxiang high tech Zone, Changsha City, Hunan Province Patentee after: Hunan Zhongjing Biotechnology Co.,Ltd. Patentee after: Hunan Animal Epidemic Prevention and Control Center Address before: Room 400-405, No.7 Panpan Road, Changsha Economic and Technological Development Zone, Hunan 410000 Patentee before: HUNAN NEW SOUTH BREEDING SERVICE Co.,Ltd. Patentee before: Hunan Animal Epidemic Prevention and Control Center |