CN111448315A - 一种Cas蛋白体系分离DNA的方法 - Google Patents

一种Cas蛋白体系分离DNA的方法 Download PDF

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CN111448315A
CN111448315A CN201880079130.5A CN201880079130A CN111448315A CN 111448315 A CN111448315 A CN 111448315A CN 201880079130 A CN201880079130 A CN 201880079130A CN 111448315 A CN111448315 A CN 111448315A
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dna
protein
cas
cas protein
solution
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CN111448315B (zh
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吴尧
王兴兴
李秋实
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Suzhou Jingrui Biotechnology Co ltd
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Suzhou Curegenetics Biotechnology Co ltd
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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    • C12N15/09—Recombinant DNA-technology
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    • C12N9/14—Hydrolases (3)
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C07K2319/00—Fusion polypeptide
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    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
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    • C07—ORGANIC CHEMISTRY
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    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/43—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a FLAG-tag
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Abstract

本发明涉及一种从溶液中分离DNA的方法，尤其涉及一种采用Cas蛋白从溶液中分离DNA的方法和试剂盒。本发明能有效提高提取游离DNA的效率。

Description

PCT国内申请，说明书已公开。

Claims (33)

  1. PCT国内申请，权利要求书已公开。
CN201880079130.5A 2018-01-03 2018-12-30 一种Cas蛋白体系分离DNA的方法 Active CN111448315B (zh)

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CN202311149195.1A CN116970601A (zh) 2018-01-03 2018-12-30 一种Cas蛋白体系分离DNA的方法

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CN2018100010730 2018-01-03
CN201810001073 2018-01-03
PCT/CN2018/125973 WO2019134630A1 (zh) 2018-01-03 2018-12-30 一种Cas蛋白体系分离DNA的方法

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CN110791578B (zh) * 2019-11-28 2022-12-16 广州微远医疗器械有限公司 用于百日咳博德特氏杆菌的crispr检测引物组及其用途
WO2022210822A1 (ja) * 2021-04-02 2022-10-06 キヤノン株式会社 試薬、及びそれを用いた標的核酸の検出方法
CN113249376A (zh) * 2021-05-18 2021-08-13 明科生物技术(杭州)有限公司 一种基于CRISPR-Cas捕获甜瓜线粒体基因组的方法及该方法的应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140357523A1 (en) * 2013-05-29 2014-12-04 Agilent Technologies, Inc. Method for fragmenting genomic dna using cas9

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WO2015120445A1 (en) 2014-02-10 2015-08-13 Nancy Quan Isolation of cell-free nucleic acids from bodily fluid samples using solid chaotropic agents
CA2971444A1 (en) * 2014-12-20 2016-06-23 Arc Bio, Llc Compositions and methods for targeted depletion, enrichment, and partitioning of nucleic acids using crispr/cas system proteins
AU2016277476B2 (en) 2015-06-10 2022-07-21 Qiagen Gmbh Method for isolating extracellular nucleic acids using anion exchange particles
CN106978428A (zh) * 2017-03-15 2017-07-25 上海吐露港生物科技有限公司 一种Cas蛋白特异结合靶标DNA、调控靶标基因转录的方法及试剂盒

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140357523A1 (en) * 2013-05-29 2014-12-04 Agilent Technologies, Inc. Method for fragmenting genomic dna using cas9

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
AMIN AALIPOUR等: "Deactivated CRISPR Associated Protein 9 for Minor-Allele Enrichment in Cell-Free DNA" *
TOSHITSUGU FUJITA等: "Efficient sequence-specific isolation of DNA fragments and chromatin by in vitro enChIP technology using recombinant CRISPR ribonucleoproteins" *
XIN LIU等: "In Situ Capture of Chromatin Interactions by Biotinylated dCas9" *

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EP3722422A4 (en) 2021-03-17
US20210095269A1 (en) 2021-04-01
CN111448315B (zh) 2023-09-22
EP3722422B1 (en) 2022-07-06
CN116970601A (zh) 2023-10-31
EP3722422A1 (en) 2020-10-14
WO2019134630A1 (zh) 2019-07-11

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