CN111418609A - Method for preventing and treating citrus canker - Google Patents

Method for preventing and treating citrus canker Download PDF

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Publication number
CN111418609A
CN111418609A CN202010034921.5A CN202010034921A CN111418609A CN 111418609 A CN111418609 A CN 111418609A CN 202010034921 A CN202010034921 A CN 202010034921A CN 111418609 A CN111418609 A CN 111418609A
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China
Prior art keywords
propolis
citrus
preventing
ethanol
canker
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Pending
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CN202010034921.5A
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Chinese (zh)
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樊金生
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Individual
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Individual
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Priority to CN202010034921.5A priority Critical patent/CN111418609A/en
Publication of CN111418609A publication Critical patent/CN111418609A/en
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Abstract

The invention discloses a method for preventing and treating citrus canker, which is characterized by being prepared from a propolis ethanol solvent. The invention is nontoxic, pollution-free, safe and effective. Provides a new method for preventing and treating citrus canker, and has wide popularization value.

Description

Method for preventing and treating citrus canker
Technical Field
The invention discloses a method for preventing and treating citrus canker, and belongs to the field of fruit tree pest control.
Background
Citrus canker is a bacterial disease that seriously harms citrus, occurs almost in all citrus producing countries throughout the world, and is one of the devastating diseases of citrus.
The disease can harm a variety of rutaceae plants, such as most species, varieties (lines) of citrus, citrus and kumquat. The citrus trees can damage leaves, branches and tips and fruits of citrus plants, and seedlings and saplings are particularly seriously damaged, so that the leaves of the plants are fallen, the branches and tips are withered, and the tree vigor is influenced and even the saplings are dead. Fruit bearing trees are damaged to cause fruit drop before picking, diseased fruits have scabs, the quality is reduced, the fruits are easy to rot in the storage period, and the commodity value of the fruits is greatly reduced.
The etiological agent of ulcer is a bacterium belonging to the genus Bacillus, gram-negative. When the temperature is proper and the humidity is high, bacteria overflow from the scab and spread in short distance by means of the interaction of wind, rain, insects and branches and leaves. The long-distance transmission mainly passes through the nursery stock with bacteria, the scion and the fruit. The germs fall onto the tender tissue of the host and invade from the air holes, water holes, skin holes and wounds. Storm and typhoon inflict a great deal of wounds on hosts, and are more beneficial to the spread and invasion of pathogenic bacteria.
ProPolis (Propolis) is a fragrant and sticky gelatinous substance obtained by collecting resin from plant buds, barks, etc. by bees, mixing with glandular secretion such as bee tongue gland and cerifera gland, and processing and transforming by bees.
A large number of domestic and foreign researches show that the propolis has broad-spectrum antibiotic effect, has a very wide antibacterial range and has good antibacterial effect on some common gram-positive bacteria and gram-negative bacteria. Propolis can accelerate cell regeneration, and can promote wound healing for ulcer.
Disclosure of Invention
The invention aims to utilize the broad-spectrum antibiotic action of the propolis, has obvious bacteriostasis to gram-negative bacteria, and utilizes the good effects of the propolis on wound regeneration of ulcer and wound healing acceleration to prevent and treat citrus canker.
The method of the invention is to spray the propolis ethanol water solution on the young shoot leaves to prevent the citrus canker. Spraying propolis ethanol water solution on the leaf with citrus canker to prevent and treat citrus canker. Soaking citrus seeds and scions in the propolis ethanol water solution for disinfection, and preventing citrus canker infection and spread caused by seed sowing and scion grafting of citrus.
Propolis is softened at 36 deg.C, has viscosity and plasticity, is dissolved at 60-70 deg.C to become viscous fluid, and becomes hard and brittle at below 15 deg.C.
The propolis dissolving process comprises the following steps:
propolis → freezing → crushing → ethanol dissolving → propolis ethanol solution
Freezing propolis is viscous at normal temperature, and is difficult to crush, so that the propolis is required to be frozen. Freezing at-18 deg.C usually makes propolis hard and brittle. Breaking the frozen propolis in time, adding broken propolis into ethanol, stirring, and dissolving to obtain propolis ethanol solvent.
The proportion of propolis to ethanol is 1-50 parts of propolis: 1-2000 parts of ethanol, and preparing the propolis ethanol solvent for later use.
The technical scheme adopted by the invention for solving the technical problems is as follows:
the method for preventing and treating the citrus canker is characterized by comprising the following steps of:
the propolis ethanol solvent is prepared into 1: 1-2000 times of water, and the propolis ethanol solvent is diluted into propolis ethanol water solution. Spraying new shoots and new fruits grown from oranges and navel oranges to prevent and treat the citrus canker; soaking seeds and scions of citrus and navel orange in propolis ethanol water solution for 10-30 min for disinfection, and preventing infection and spread of citrus canker during sowing and grafting of the seeds.
The invention has the beneficial effects that: the propolis ethanol aqueous solution is used for preventing and treating the citrus canker, a new method for preventing and treating the citrus canker is developed, pesticide pollution and residue are avoided, and the propolis aqueous solution is non-toxic, pollution-free, safe and effective. Has great significance for promoting the development of the citrus industry.
The method is novel, scientific and reasonable, pollution-free and has wide popularization value.
Detailed Description
1. Freezing propolis 2 kg in freezer at-18 deg.C for 48 hr to make propolis hard and brittle, taking out, breaking, dissolving in 2000 kg ethanol for 40 days, and making into propolis ethanol solvent. Mixing propolis ethanol solvent with 200 times of water to obtain propolis ethanol water solution. Spraying leaves and young fruits of citrus trees suffering from canker;
2. freezing propolis 2 kg in freezer at-18 deg.C for 48 hr to make propolis hard and brittle, taking out, breaking, dissolving in 2000 kg ethanol for 40 days, and making into propolis ethanol solvent. Mixing propolis ethanol solvent with 200 times of water to obtain propolis ethanol water solution. Spraying leaves and young fruits of navel orange trees suffering from canker;
3. freezing propolis 2 kg in freezer at-18 deg.C for 50 hr to make propolis hard and brittle, taking out, breaking, dissolving in 1500 kg ethanol for 50 days, and making into propolis ethanol solvent. Mixing propolis ethanol solvent with 300 times of water to obtain propolis ethanol water solution. Soaking citrus seeds and scions in the propolis ethanol water solution for 10-30 minutes for disinfection;
4. freezing propolis 2 kg in freezer at-18 deg.C for 50 hr to make propolis hard and brittle, taking out, breaking, dissolving in 1500 kg ethanol for 50 days, and making into propolis ethanol solvent. Mixing propolis ethanol solvent with 300 times of water to obtain propolis ethanol water solution. Soaking navel orange seed and scion in propolis ethanol water solution for 10-30 min for disinfection.

Claims (5)

1. A method for preventing and treating citrus canker is characterized in that the citrus canker is prepared from propolis and an ethanol solvent.
2. A method of controlling citrus canker according to claim 1, wherein citrus refers to all citrus varieties including citrus, citrus navel orange varieties, kumquat, citrus grocery, and the like.
3. A method for controlling citrus canker according to claim 1, characterized in that the content of propolis is 1-100%.
4. A method for preventing and treating citrus canker according to claim 1, characterized in that the purity of the ethanol in which propolis is dissolved is 10 to 99%, and the weight of the ethanol is 1 to 1000 times the weight of propolis.
5. A method for preventing and treating citrus canker, according to claim 1, characterized in that the amount of water blended with the propolis ethanol solvent is 1 to 2000 times the weight of the propolis ethanol solvent.
CN202010034921.5A 2020-01-08 2020-01-08 Method for preventing and treating citrus canker Pending CN111418609A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202010034921.5A CN111418609A (en) 2020-01-08 2020-01-08 Method for preventing and treating citrus canker

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010034921.5A CN111418609A (en) 2020-01-08 2020-01-08 Method for preventing and treating citrus canker

Publications (1)

Publication Number Publication Date
CN111418609A true CN111418609A (en) 2020-07-17

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CN202010034921.5A Pending CN111418609A (en) 2020-01-08 2020-01-08 Method for preventing and treating citrus canker

Country Status (1)

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CN (1) CN111418609A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114711079A (en) * 2022-04-20 2022-07-08 陈斌 Prevention and treatment method for citrus canker

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1465569A (en) * 2002-06-25 2004-01-07 杨汝伟 Method for raising total flavone content in propolis
CN110250207A (en) * 2019-06-19 2019-09-20 浙江万里学院 A kind of citrus brown spot pathogenic bacteria bacteriostatic agent and preparation method thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1465569A (en) * 2002-06-25 2004-01-07 杨汝伟 Method for raising total flavone content in propolis
CN110250207A (en) * 2019-06-19 2019-09-20 浙江万里学院 A kind of citrus brown spot pathogenic bacteria bacteriostatic agent and preparation method thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ESIN BASIM ET AL.: "Antibacterial activities of Turkish pollen and propolis extracts against plant bacterial pathogens", 《JOURNAL OF FOOD ENGINEERING》 *
杨书珍 等: "蜂胶提取物处理对柑橘诱导抗病性的影响", 《食品科学》, vol. 31, no. 08, pages 275 - 279 *
阿米尼姑丽•买买提 等: "新疆产蜂胶提取物对农作物病原真菌的抗性作用", 《新疆农业科学》, vol. 48, no. 9, pages 1716 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114711079A (en) * 2022-04-20 2022-07-08 陈斌 Prevention and treatment method for citrus canker

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