CN1465569A - Method for raising total flavone content in propolis - Google Patents
Method for raising total flavone content in propolis Download PDFInfo
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- CN1465569A CN1465569A CNA021351392A CN02135139A CN1465569A CN 1465569 A CN1465569 A CN 1465569A CN A021351392 A CNA021351392 A CN A021351392A CN 02135139 A CN02135139 A CN 02135139A CN 1465569 A CN1465569 A CN 1465569A
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- propolis
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- flavone content
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Abstract
The present invention provides a method for raising total flavone content of propolis, and said method includes the following steps: freeze-pulverizing raw material, water-bathing and removing impurities, freeze-pulverizing solvent and extracting and vacuum drying so as to obtain dried extract product high-content total flavone propolis.
Description
1, technical field the present invention relates to a kind of deep processing method of bee product, specifically a kind of method that improves total flavone content in propolis.
2, technical background
Propolis (PR0PLIS) Resin adhesive, the pollen the bud and honeybee mandibular gland secretory product and beeswax that to be honeybee gather from plant plumule and trunk mix; Be the mixture that a kind of utmost point has the crude substance of economic worth, compositions such as flavonoid, terpenes, organic acid, polyose wherein, above-mentioned composition is known as total flavones.Total flavones in the propolis is through biologist, nutritionist, physician and chemist's years of researches exploration discovery, the total flavones of propolis has the various biological effect, people's physical efficiency is played antibacterial, sterilization, adjusting immunity, regulating blood fat, regulate blood viscosity, reduce effects such as vascular fragility, its pharmaceutical use and healthy nutritive value are familiar with by people gradually and are accepted.
Be propolis to be extracted at the beginning of 21 century by ethanol or diethyl ether solution.Though the said extracted method can obtain most of extract, in the process of later stage removal of impurities, be difficult for material is pulverized because of containing beeswax in the propolis during merely with the organic solvent lixiviate, and easily form agglomerate because of propolis and be difficult to the total flavones in the propolis is proposed fully.Generally be that water-bath removal of impurities water is discarded in the propolis deep-processing process of prior art, thereby cause a large amount of wastes of water-soluble flavone and cause the reduction of effective active components Flavonoid substances in the propolis, reduced the propolis product edibleness and exploitation is worth.
3, goal of the invention
The method that the purpose of this invention is to provide a kind of effective raising total flavone content in propolis.
The objective of the invention is to realize in the following manner,, the extracting method of prior art is improved, adopted multiple, coherent, easy effectively purification mode, make that the total flavones yield increases in the propolis according to the existing in prior technology deficiency.This method is to be based upon the coarse raw materials propolis that is mixed with impurity such as beeswax, honeybee corpse, wood chip, sandstone is carried out multipass cryogenic freezing, pulverizing, water-bath removal of impurities and organic solvent extraction to obtain the high-load propolis of the finished product total flavones, can remove quality that impurity improves propolis product effectively by method of the present invention and can less lose water-soluble flavone class material in the propolis again, keep higher edibleness and exploitation value.
A kind of method that improves total flavone content in propolis of the present invention comprises water-bath removal of impurities, preparing solvent and extraction and the vacuum-drying of propolis:
(a) the water-bath removal of impurities of propolis: be with the propolis coarse raw materials place-5 ℃ freezing 24 hours, pulverizing the 60-100 order slowly is sieved in the boiling water of interlayer heating kettle, constantly stir, feeding intake finishes continues heating 5-10 minute, stop to heat naturally cooling, impurity in the propolis solidifies one with beeswax gradually, treat to solidify fully the back and take out beeswax and attached impurity thereof, the aqueous solution filters standby, the propolis that the bottom of a pan is frozen into agglomerate takes out and to place again-low temperature of 5C under freezing 24 hours, pulverizing 60 mesh sieves, to make the propolis powder standby, and the aqueous solution filters standby.
(b) preparing solvent and extraction: the aqueous solution after the water-bath and organic solvent are made into mixed solvent by 1: 3 mixed, and the propolis powder adds in the mixed solvent and stirred vacuum filtration, waste, filtrate for later use 24-72 hour.
(c) vacuum-drying: by carrying out steam heating vacuum-drying in the vacuum contact loft drier, the organic solvent in the material adopts to reclaim in the dividing wall type condenser and reuses, and gets the high propolis dry extract product of general flavone content after above drying with suction filtration liquid.
The used organic solvent of effective ingredient extraction is one or more a mixture of ethanol, ether, ethyl acetate, acetone in the propolis in the method for the invention.
4 embodiments:
The specific embodiment of method of the present invention is as follows:
The first step: the propolis coarse raw materials was placed under-5 ℃ of temperature freezing 24 hours, raw material solidifies the back to be pulverized with pulverizer, slowly be sieved into the 60-100 mesh sieve in the boiling water of interlayer heating kettle, constantly stir, feeding intake finishes continues heating 5-10 minute, stop to heat naturally cooling, impurity in the propolis solidifies one with beeswax gradually, treat to solidify fully the back and take out beeswax and attached impurity thereof, propolis freezes solidly on the bottom of a pan than great form with agglomerate, the propolis agglomerate taken out place again-low temperature of 5C under freezing 24 hours, it is standby to pulverize 60 mesh sieves again, the aqueous solution filters standby.
Second step: the aqueous solution after the water-bath and organic solvent are made into mixed solvent by 1: 3 mixed, dissolve stirring 24-72 hour, vacuum filtration, filtrate for later use in the propolis powder adding mixed solvent after will pulverizing then.
The 3rd step: suction filtration liquid is poured in the charging tray of vacuum contact loft drier, carry out steam heating vacuum-drying, organic solvent in the material adopts to reclaim in the dividing wall type condenser and reuses, and gets the high propolis dry extract product of general flavone content after above drying.
Propolis extracting method of the present invention, its major technique feature are to carry out certain polarization or non-polarized processing according to the extract product quality requirement.The two or more mixed organic solvents of general employing carries out the extraction of propolis to obtain the propolis product of the high yield of total flavones.
The objective of the invention is to propose the method for the high yield of a kind of propolis total flavones, with present propolis product Deep process technology is further simplified and is optimized, and is conducive to medium-sized and small enterprises to the deep processing of propolis product resource and opens Send out to utilize, method of the present invention be compared with existing technology technological design rationally, general flavone content in the propolis product The characteristics such as high, product purity is high, easy and simple to handle have good value for applications.
Claims (2)
1. a method that improves total flavone content in propolis comprises water-bath removal of impurities, preparing solvent and extraction and the vacuum-drying of propolis, it is characterized in that
(a) the water-bath removal of impurities of propolis: be that the propolis coarse raw materials was placed under-5 ℃ of temperature freezing 24 hours, raw material solidifies the back to be pulverized with pulverizer, slowly be sieved into the 60-100 mesh sieve in the boiling water of interlayer heating kettle, constantly stir, feeding intake finishes continues heating 5-10 minute, stop to heat naturally cooling, impurity in the propolis solidifies one with beeswax gradually, treat to solidify fully the back and take out beeswax and attached impurity thereof, propolis freezes solidly on the bottom of a pan than great form with agglomerate, the propolis agglomerate taken out place again-low temperature of 5C under freezing 24 hours, pulverizing 60 mesh sieves again, to make the propolis powder standby, the aqueous solution filters standby.
(b) preparing solvent and extraction: the aqueous solution after the water-bath and organic solvent are made into mixed solvent by 1: 3 mixed, and the propolis powder adds in the mixed solvent and stirred vacuum filtration, waste, filtrate for later use 24-72 hour.
(c) vacuum-drying: by carrying out steam heating vacuum-drying in the vacuum contact loft drier, the organic solvent in the material adopts to reclaim in the dividing wall type condenser and reuses, and gets the high propolis dry extract product of general flavone content after above drying with suction filtration liquid.
2, a kind of method that improves total flavone content in propolis according to claim 1 is characterized in that extracting used organic solvent and is one or more mixture of ethanol, ether, ethyl acetate, acetone.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02135139 CN1260219C (en) | 2002-06-25 | 2002-06-25 | Method for raising total flavone content in propolis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02135139 CN1260219C (en) | 2002-06-25 | 2002-06-25 | Method for raising total flavone content in propolis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1465569A true CN1465569A (en) | 2004-01-07 |
| CN1260219C CN1260219C (en) | 2006-06-21 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 02135139 Expired - Lifetime CN1260219C (en) | 2002-06-25 | 2002-06-25 | Method for raising total flavone content in propolis |
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| CN (1) | CN1260219C (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107224445A (en) * | 2017-05-08 | 2017-10-03 | 西北大学 | Propolis active component and its compound product and application |
| CN107964000A (en) * | 2017-11-28 | 2018-04-27 | 张夏洋 | The double assisted extraction beeswax flavones of enzyme-ultrasound |
| CN108619084A (en) * | 2018-04-25 | 2018-10-09 | 四川生命源健康管理有限公司 | A kind of anti-ageing nourishing gel of female private part |
| CN108677530A (en) * | 2018-05-15 | 2018-10-19 | 张彦 | antibacterial finishing agent and its preparation method and application |
| CN109528843A (en) * | 2018-11-28 | 2019-03-29 | 河北科技大学 | A kind of extracting method of flavone of hawthorn fruit |
| CN111418609A (en) * | 2020-01-08 | 2020-07-17 | 樊金生 | Method for preventing and treating citrus canker |
| CN114224920A (en) * | 2022-01-24 | 2022-03-25 | 吉林蜂道馆健康产业有限公司 | Bee therapy product with sterilizing and inflammation diminishing functions and preparation method thereof |
-
2002
- 2002-06-25 CN CN 02135139 patent/CN1260219C/en not_active Expired - Lifetime
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107224445A (en) * | 2017-05-08 | 2017-10-03 | 西北大学 | Propolis active component and its compound product and application |
| CN107224445B (en) * | 2017-05-08 | 2020-05-15 | 西北大学 | Propolis effective part and compound product and application thereof |
| CN107964000A (en) * | 2017-11-28 | 2018-04-27 | 张夏洋 | The double assisted extraction beeswax flavones of enzyme-ultrasound |
| CN108619084A (en) * | 2018-04-25 | 2018-10-09 | 四川生命源健康管理有限公司 | A kind of anti-ageing nourishing gel of female private part |
| CN108677530A (en) * | 2018-05-15 | 2018-10-19 | 张彦 | antibacterial finishing agent and its preparation method and application |
| CN108677530B (en) * | 2018-05-15 | 2021-06-01 | 新乡橄榄枝纺织化工有限公司 | Antibacterial finishing agent and preparation method and application thereof |
| CN109528843A (en) * | 2018-11-28 | 2019-03-29 | 河北科技大学 | A kind of extracting method of flavone of hawthorn fruit |
| CN111418609A (en) * | 2020-01-08 | 2020-07-17 | 樊金生 | Method for preventing and treating citrus canker |
| CN114224920A (en) * | 2022-01-24 | 2022-03-25 | 吉林蜂道馆健康产业有限公司 | Bee therapy product with sterilizing and inflammation diminishing functions and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1260219C (en) | 2006-06-21 |
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Granted publication date: 20060621 |