KR20090056442A - Chlorophyll Extraction Method - Google Patents

Abstract

The present invention relates to a method for extracting chlorophyll, comprising the steps of collecting, washing and dipping chlorophyll-containing edible plants in 12% to 14% of brine and degrading the organic and carotene components of the plant's envelope using crystal soda. Prevents denaturation of chlorophyll, crushes it in a blender, separates the pulverized and starch containing precipitated chlorophyll, mixes the starch in ethanol (95%), and matures for 7-8 days to filter chlorophyll. The extracting step and evaporation of the spirits to extract chlorophyll completely extracts and concentrates, thus providing a method for extracting pure chlorophyll without degeneration.

Description

Chlorophyll Extraction Method {the way of abstraction of chlorophyll}

The present invention relates to a method for extracting pure chlorophyll free of denaturation.

Chlorophyll (chlorophyll) is a beneficial substance to the human body to prevent cancer and clear blood, such as hematopoiesis, sterilization, expansion of microvascular and acceleration of metabolism, antiallergic action. Chlorophyll can heal wounds of the intestine, and has granulation regeneration, which heals the wounded cells and restores them to their original state.

Therefore, ingesting a lot of chlorophyll will heal the wounds of the stomach and strengthen the intestines, which in turn can give a good effect on the entire immune system of the human body, which can help prevent and treat cancer. Chlorophyll, on the other hand, is a pigment that coexists with carotenoids in the chloroplast, which is rich in nutrients, and determines the freshness of green leaf plants.

It is relatively stable and preserved when there is vitality of the plant, but when the vitality is lost, chlorophyll is browned and discolored to pheophytin due to carotene components and various organic acid enzymes contained in chloroplasts.

For this reason it is also not important to extract chlorophyll from plants, but it is also important that the chlorophyll is able to maintain vitality without discoloring for a long time. As described above, despite the beneficial effects of chlorophyll on the human body, there is no way to extract pure chlorophyll, so far, it has been limited to the method of directly eating raw foods or foods such as green tea, kale or wormwood. Although the amazing effects of chlorophyll have been demonstrated in various studies and clinical trials for over 100 years, the development of methods for extracting pure chlorophyll has been insufficient.

Recently, a method of extracting chlorophyll (Korean Patent Registration No. 10-0451566) has been invented, but problems have arisen in the process of evaporating the extraction solvent contained in the drying step of the chlorophyll and chlorophyll extraction. In the drying stage of green leaf plants, it takes 10 to 20 days in the shade. This method does not consider the fact that during the drying stage, it is oxidized to carotene and air and discolors and destroys a large part of chlorophyll. will be.

And in order to evaporate the extraction solvent contained in the chlorophyll extraction process is proposed to obtain a chlorophyll concentrate by heating to 30 ℃ ~ 40 ℃, the extraction solvent is sufficiently evaporated by a simple method of applying heat of 30 ℃ ~ 40 ℃ The disadvantage is that it is not time consuming.

Another recently invented method for producing chlorophyll (Korean Patent Registration No. 10-0348650) also presents an important problem.

According to the present invention, chlorophyll extracted from the final process and crystal soda are mixed and heated at 90 ° C. to prevent the release of magnesium (Mg) factor in the chlorophyll, thereby maintaining the chlorophyll life for a long time.

This method is concerned that chlorophyll can be destroyed in a large amount by applying heat at 90 ° C, and it is difficult to maintain pure chlorophyll because the fat-soluble chlorophyll is changed to water-soluble. In addition, magnesium (Mg) factor is also zinc-based, it can be destroyed by the alkaline soda crystals, it is difficult to maintain the life of chlorophyll for a long time.

The present invention has been made in order to solve such a conventional problem, and an object of the present invention is to provide a method for extracting chlorophyll in high yield and a method for maintaining the vitality of the extracted chlorophyll long-term without denaturation.

In order to achieve the above object, the present invention collects and washes chlorophyll-containing plants and soaks them in 12% to 14% of brine for 7 to 8 hours and crystallizes them (sodium carbonate 40%, sodium bicarbonate 5%). , The solution of 55% water) is dissolved in warm water (80 ℃ ~ 85 ℃) dissolved 7 ~ 8% to disintegrate the outer skin of the plant and remove the carotene and crush it in the blender. Starch containing is separated. This is filtered to obtain starch containing chlorophyll.

This was put into a hard glass jar maintained at 10 ° C. to 15 ° C. with ethanol as an extraction solvent, and stirred 7 times to 8 times a day while sufficiently extracting, ripening, and filtering starch for 8 days to 10 days. After removal and obtaining chlorophyll-containing ethanol, it is characterized in that the step consisting of evaporating the ethanol and obtaining chlorophyll to concentrate.

As described above, chlorophylls are fermented by salting, chlorophyll dyeing and chlorophyll staining by chlorophyll dyeing to remove chlorophylls without discoloration. Intake is effective.

As a plant used for extracting chlorophyll by this invention, any chlorophyll-containing green leaf plant which can be eaten can be used. In addition to the leaf part, the stem part can be used. These plants are harvested and washed with clean water.

Soak in brine (12% to 14%) and let it age for 8-10 hours. (If it is dried in salt water for more than 10 days, the chlorophyll may be destroyed by oxidation of flavonoid-based carotene and air.) Soaking in salt water and aging can reduce the volume of chlorophyll raw material. It is convenient and has affinity with chlorophyll, which has salt (magnesium chloride) and magnesium (Mg) factors, so that the color of leaves and stems can be preserved for a long time.

After aging chlorophyll raw material as above, crystalline sodium (40% sodium carbonate, 5% sodium bicarbonate, 55% water mixed) dissolved in warm water (80 ℃ ~ 85 ℃) is dissolved in a fixed amount.

Here, the organic components, which are the envelope of the leaves and stems of plants, are dissolved (decomposed) by crystal soda. At the same time, the outer membrane is removed to remove flavonoid-based carotene.

If carotene is contained in the chloroplast, chlorophyll will be browned, fade and lose its vitality, so carotene should be removed.

The chlorophyll raw material (leaves and stems) blanched as above is washed four to five times in clean water to remove dissolved crystalline soda and water-soluble carotene.

The envelope of the plant is composed of organic acids. Therefore, the action of crystalline sodium is to remove the outer membrane to help the chlorophyll extraction process, and when chlorophyll is combined with acid, it becomes a peopyrine and neutralizes organic acid to brown and fade, thus removing carotene to prevent chlorophyll degeneration in advance. Do.

Crystalline soda is alkaline and the outer shell of the body decomposes the organic components and at the same time plays a role in removing the carotene component which is a family of flavonoid pigments. Flavonoids are water-soluble yellow pigments widely distributed in plants and are unstable in alkalinity.

In other words, dissolve the crystal soda in 7 ~ 8% in a suitable amount of warm water (80 ℃ ~ 85 ℃) and add the leaves and stems of the salted leaf plant to boil it. As the components disintegrate, the water-soluble carotene of the flavonoid family contained in the chloroplast, which causes chlorophyll discoloration, is also removed. The chlorophyll raw material (leaf) thus obtained is rinsed four to five times in clean water to remove dissolved crystalline sodium sorbate and water-soluble carotene.

The chlorophyll raw material obtained as described above is dehydrated and compressed to finely pulverize while injecting an appropriate amount of distilled water into a skew mixer (5 mm), which is filtered with a mesh size of 10 to 20 mesh.

At this time, if it is less than 10 mesh, chlorophyll may be destroyed, and if it is larger than 20 mesh, it may be difficult to extract high yield of chlorophyll in a subsequent process.

Through the grinding process, starch containing fiber and chlorophyll is separated.

After 10 hours, the large particles of fiber are taken out with a 10% cooker and small starch is precipitated. The supernatant is removed and filtered through a fine filter cloth to obtain hemirose starch containing chlorophyll. The remaining moisture in the starch is removed and dried to form a starch mass.

This is pulverized and mixed with ethanol (95%) as an extraction solvent to allow chlorophyll extraction. Any solvent can be used as long as it can extract chlorophyll, but ethanol is preferable. In the case where the extract uses leaves as the main raw materials, the mixing ratio of the chlorophyll-containing starch and the extraction solvent added to the extraction jar is preferably 40:60 by weight.

As described above, in order to increase the extraction efficiency of chlorophyll in the process of extracting chlorophyll from chlorophyll-containing starch, chlorophyll-containing starch and ethanol (95%) as an extraction solvent were mixed in a ratio of 40:60 and placed in a hard glass jar for 8 days. Chlorophyll is extracted by aging for ~ 10 days.

At this time, stir at the beginning of the ripening process (for 3-4 hours) at intervals of 4-5 minutes, and then stir 7-8 times a day to increase the extraction efficiency. In this process, chlorophyll, which is contained in starch by osmotic principle, is dissolved and extracted in ethanol.

As such, the reason for aging for a long period of 8 to 10 days is to allow the chlorophyll to be sufficiently dissolved in the extraction solvent by osmotic pressure and to make the distribution of chlorophyll dissolved in the extraction solution uniform.

In this case, the aging temperature is preferably 10 ° C. to 15 ° C., but when aged outside the temperature, the extraction solvent may evaporate from the extract.

If the extraction solvent evaporates during the aging process, chlorophyll cannot be protected and chlorophyll is destroyed. And since the temperature is lowered below 10 ℃ chlorophyll reacted to the fat-soluble because it does not melt at low temperatures it is impossible to extract.

As a result of aging extraction, the resultant is filtered with a fine filter to separate and remove starch, thereby obtaining ethanol containing chlorophyll extract.

10 cc of distilled water is added to 1 liter of chlorophyll-containing ethanol, placed in a rinse bottle, and placed in hot water at 60 ° C to 70 ° C. If it is lower than 60 ℃ has a disadvantage that takes a lot of time, and if it is 70 ℃ or more there is a risk that chlorophyll is destroyed due to the high temperature.

When ethanol is evaporated and subsequently cooled (5 ° C.), chlorophyll solidifies and floats on water because it is reactive and fat-soluble. The collected chlorophyll is washed with distilled water to remove impurities to obtain pure chlorophyll.

In the following will be described by taking an example of the mugwort study.

<Example>

Mugwort was harvested, washed thoroughly and soaked in 12% brine for 8 hours.

Worm salted aged mugwort in 85 ° C warm water in which 8% of soda was dissolved. Here, the organic components, which are the outer coating of mugwort leaves and stems, are dissolved, and at the same time, water-soluble carotene is dissolved. It was washed five times with clean water to remove dissolved soda, organic component and carotene.

● The water of the mugwort was removed, compressed and ground in a 5 mm skew mixer. The pulverized starch of wormwood containing fiber and chlorophyll was separated to obtain starch containing chlorophyll.

6 g of ethanol (95%) was mixed in 500 g of wormwood containing chlorophyll, and placed in a hard glass jar and stirred for 8 days while stirring 6-7 times a day at a temperature of 10 ° C. to 15 ° C. In the process, chlorophyll was extracted and dissolved in alcohol. This was filtered through a filter to remove starch to obtain a spirit in which chlorophyll was dissolved.

10 cc of distilled water was added to 1 liter of chlorophyll dissolved in a rinse bottle, and the mixture was evaporated and cooled by rotating at 70 ° C in warm water. Chlorophyll, which had solidified in distilled water, was filtered and discarded.

The collected chlorophyll was washed with distilled water to remove impurities and to obtain pure chlorophyll.

Claims (3)

Chlorophyll-containing plants are collected, washed and soaked in 12% to 14% brine for 7-8 hours, followed by 7-8% crystallized sodium (40% sodium carbonate, 5% sodium bicarbonate, 55% water). Pouring the melted hot water (80 ℃ ~ 85 ℃) to disintegrate the outer skin of the plant, remove carotene to prevent chlorophyll degeneration, and grind in a blender to separate the fiber and chlorophyll-containing starch, and ethanol and starch (chlorophyll) It is added to the hard glass jar maintained at 10 ℃ ~ 15 ℃ in a weight ratio of 6: 4 and aged for 8 to 10 days to extract the starch and ethanol evaporated and cooled to obtain chlorophyll Chlorophyll extraction method. The method of claim 1, Chlorophyll-containing plants are leaves of wormwood and the ratio of starch and extractant of wormwood is 4: 6 chlorophyll extraction method characterized in that the weight ratio. The method of claim 1, Chlorophyll-containing plant is a chlorophyll extract method characterized in that at least one selected from the leaf portion, stem portion of the edible plant, including ginkgo leaves, pine needles, mulberry leaves, kelp.