CN111357425A

CN111357425A - Experimental tool for germination percentage statistics and root length statistics

Info

Publication number: CN111357425A
Application number: CN201811588665.3A
Authority: CN
Inventors: 王玲; 刘玉佳; 李亚楠
Original assignee: Northeast Forestry University
Current assignee: Northeast Forestry University
Priority date: 2018-12-25
Filing date: 2018-12-25
Publication date: 2020-07-03
Anticipated expiration: 2038-12-25
Also published as: CN111357425B

Abstract

The invention relates to an experimental tool for counting the germination rate and root length of seeds. The technical scheme is as follows: the front surface of the tool comprises a base with a hollow layer, the hollow layer of the base, a concave part of the base and an inserting plate clamped in the hollow layer of the base. The base with the hollow layer is square, the hollow layer of the base is rectangular, the concave part of the base is in a perfect circle shape, and the inserting plate clamped in the hollow layer of the base is in an L shape. When dibbling in the culture dish, go on according to different partition equally on the picture peg, use hollow point or solid point according to quantity, not only the statistical data of being convenient for but also do benefit to and shoot. The reverse side of the tool comprises a base, positioning points on the base, a transparent bottom plate with a graduated scale and grid lines and a fully transparent bottom plate. The base and the bottom plate are both square, and the positioning points on the base are cylinders. When the root length is counted, the placing position of the root can be determined by the graduated scale and the grid lines, data can be displayed visually, and the bottom plate can be used independently or uniformly. The invention can not only accurately position in the statistical experiment, but also save time.

Description

Experimental tool for germination percentage statistics and root length statistics

Technical Field

The invention relates to an experiment auxiliary tool used in statistical experiments of seed germination rate and root length.

Background

The statistical data of the seed germination rate and the measurement data of the fresh weight and root length of seedlings are basic experimental contents included in many experimental designs, for example, the resistance difference of seeds and wild seeds under different conditions, the data information of the conventional seed germination rate and the like, and the dibbling operation of the seeds on a culture medium is required. The subsequent seedling stage requires statistical analysis of the root length data of different lines of seedlings (commonly used for wild-type seedlings and transgenic seedlings in the experiment) under different concentration gradient treatments. The experimental conclusion needs to be supported by the contents of the characters and the pictures, so that the experimental contents are perfected by a relatively tidy and beautiful experimental picture regardless of the seed arrangement for counting the germination percentage or the seedling arrangement for counting the root length. The position of point seeds and seedling arrangement is judged artificially and subjectively, the boundary of seeds or seedlings in different areas is not clear, or the seeds or seedlings in the same row (the same column) are not on the same horizontal line (vertical line), so that the statistics of germination percentage or the judgment of root length is directly influenced, and the attractiveness of an experimental picture is also influenced.

Disclosure of Invention

The invention aims to provide an invention tool convenient for experiment development aiming at the inconvenience of operation of an experimenter in the experiment process, which is not only convenient for the experimenter to accurately determine the position of seeds or seedlings in the experiment and obtain high-quality experiment data, but also saves the time of an experiment operator and is beneficial to the efficient development of the experiment.

The technical scheme is as follows: the instrument is compound two-sided, openly including the base that has the cavity, the cavity of base, the concave part of base, the picture peg of card putting in the cavity of base, the base outline that has the cavity of cavity is the square, and the cavity of base outline is the rectangle, and the concave part of base divide into four just circular recesses that are used for fixed culture dish that the equidistance was arranged, and the picture peg outline of card putting in the cavity of base is L shape. The back side of the base comprises a base, positioning points on the base, a transparent bottom plate with a graduated scale and grid lines and a fully transparent bottom plate, wherein the outlines of the base and the bottom plate are square.

The tool comprises a base, a hollow layer, a side length of a square outline on the outer side of the base is 300mm, the height of the base is 18mm, an outer contour of the hollow layer of the base is 296 mm-298 mm rectangle, the thickness of the base is 3mm, four circular radii of the concave part of the base are 45mm, the depth of the concave part is 5mm, centers of the four circular circles are 75mm away from the edge of the outer contour of the base and can be connected into a square, the side length of the inserting plate clamped on the hollow layer of the base is 150mm and is L-shaped, an outer contour of the long side is 296 mm- × mm rectangle, the thickness of the inserting plate is 2mm, an outer contour of the short side is 296 mm- × mm rectangle, the thickness of the inserting plate clamped on the hollow layer of the base is 7mm, four circular regions are uniformly distributed on the inserting plate, the radius of the inserting plate is 45mm, the full length of the full region, the full length of the full row, the full row and full row of the full circle of the four circular dots are 2mm, the full circle of the full circle, the full circle of the full circle, the full circle of the full circle, the full circle of the full circle, the full circle of the full circle, the full circle of the full circle.

The base of the tool is made of an acrylic plate, has the characteristics of high plasticity, good permeability, strong bearing capacity, light dead weight, easiness in cleaning and the like, and greatly meets various requirements in the using process. The insert plate is made of acrylic plates, four kinds of perfect circular lines and hollow and solid round points which are used as point seed references are printed on the insert plate, and the four kinds of perfect circular lines and the hollow and solid round points are black. The material of transparent bottom plate is the ya keli board, can guarantee like this that the bottom plate is enough transparent, according to the demand of shooing of difference, the bottom plate can superpose the use.

The invention has the beneficial effects that: when a dibbling experiment is carried out in a culture dish with a poured culture medium, different regions (whole region, second partition, third partition and fourth partition) and different quantities (selecting reference hollow points and solid points for separate use or all use) can be selected for dibbling according to set experiment requirements by referring to regions and reference points which are defined by inserting plates which are clamped in hollow layers of a base, so that the seeds are ensured to be arranged orderly, high-quality pictures can be obtained, the quantity of the hollow points and the solid points is fixed, and the statistics of experiment data is facilitated. Meanwhile, the culture dish can be firmly fixed by the circular groove of the concave part of the base, and the culture dish can accurately correspond to the area and the reference point defined on the inserting plate by adjusting one side of the short edge of the L-shaped inserting plate. When the root length of seedlings is compared and counted, the bottom plates are placed according to different requirements, the placing position of each strain seedling is accurately grasped by using the reference lines, and the transparent bottom plate with the graduated scale and the grid lines can more visually display data of an experimenter in an experiment report or a paper. According to the needs of shooing of difference, can the arbitrary bottom plate of exclusive use, also can superpose the use, in order to make the photo more clear, can also insert the suitable size at the cavity layer of base and take the colour card paper (mostly black) as the photo ground colour.

In the statistical experiment of the germination rate of the seeds and the root length of the seedlings, the invention can accurately determine the positions of the seeds or the seedlings on one hand, and can save the time of an experiment operator on the other hand, thereby being beneficial to the development of the experiment. In consideration of the design of the experiment, the data of the germination rate of the seeds and the data of the fresh weight and root length of the seedlings are the basic parts of the same experiment, and meanwhile, the invention tool meeting the two experiment operations is provided, so that experimenters can complete the experiment contents more efficiently.

Drawings

FIG. 1 is a top plan view of the front base of the tool;

FIG. 2 is a top view of a first tool face insert plate;

FIG. 3 is a top view of a second tool front insert plate;

FIG. 4 is a cross-sectional view α - α of FIG. 1;

FIG. 5 is a cross-sectional view of β - β in FIG. 2 and γ - γ in FIG. 3;

FIG. 6 is a top view of the reverse base of the tool;

FIG. 7 is a top view of a transparent base plate with a scale and grid lines on the reverse side of the tool;

FIG. 8 is a top view of the tool reverse side fully transparent base plate;

FIG. 9 is a sectional view taken along line A-A of FIG. 6;

fig. 10 is a cross-sectional view of B-B in fig. 7 and C-C in fig. 8.

In the upper diagram: the front surface of the tool comprises a base 1 with a hollow layer, a base hollow layer 2, a concave part 3 of the base, a first plug board 4 clamped in the base hollow layer,

different seeding areas

5, 6, 7 and 8 on the first plug board, a second plug board 9 clamped in the base hollow layer and

different seeding areas

10, 11, 12 and 13 on the second plug board. The reverse side of the tool comprises a base 14, positioning points 15 on the base, a transparent bottom plate 16 with a graduated scale and grid lines and a fully transparent bottom plate 17.

Detailed Description

The preferred embodiments of the present invention will be described in conjunction with the accompanying drawings, and it will be understood that they are described herein for the purpose of illustration and explanation and not limitation.

The outline of a base 1 with a hollow layer on the front surface of the invention is square, the outline of a base hollow layer 2 is rectangular, the outline of a concave part 3 of the base is perfect circle, and the outline of a first plug board 4 clamped in the base hollow layer and the outline of a second plug board 9 clamped in the base hollow layer are L-shaped. According to different dibbling requirements, dibbling experiments can be carried out according to different partitions on the plugboard, four different

dibbling areas

5, 6, 7 and 8 which are all in a perfect circle are equidistantly distributed on the first plugboard 4, and four different

dibbling areas

10, 11, 12 and 13 which are all in a perfect circle are equidistantly distributed on the second plugboard 9. According to different experimental requirements, different seeding areas are selected. The reverse side of the invention is that the base 14 is square, and the positioning points 15 on the base are four cylinders fixed on four corners of the base 14. The transparent bottom plate 16 with the four sides provided with the graduated scale and the grid lines and the fully transparent bottom plate 17 are respectively provided with four holes matched with the positioning points 15 on the base, and the holes are clamped into the cylinder for fixing when the device is used. According to different photographing requirements, the transparent bottom plate 16 with the graduated scale and the grid lines, the fully transparent bottom plate 17 or both can be replaced for use.

Referring to fig. 1-5, on the front side of the tool, the outer contour of the base 1 with the hollow layer is square, the side length is 300mm, the outer contour of the base hollow layer 2 is rectangular, the side length is 296mm × 298mm, the thickness is 3mm, the outer contour of the concave part 3 of the base is perfect circle, the radius is 45mm, the depth is 5mm, the center of the circle is 75mm from the edge and can be connected into a square, the side length is 150mm, the outer contour of the first insert plate 4 clamped in the base hollow layer and the second insert plate 9 clamped in the base hollow layer is L-shaped, the long side is 296mm × 298mm rectangular, the thickness is 2mm, the short side is 296mm × 5mm, the thickness is 7mm, four different

point type areas

5, 6, 7, 8 are perfect circle, the radius is 45mm, the two insert plate 9 are equidistantly distributed with four different

point type areas

10, 11, 12, 13, are perfect circle, the radius is 45mm, each area is arranged according to the number of reference line, the hollow layer is arranged, the hollow layer is 1mm, the hollow layer is arranged with the hollow layer, the hollow layer is arranged with the circle, the hollow layer is arranged with the hollow layer, the hollow.

Referring to fig. 6-10, the outer contour of the base 14 on the reverse side of the tool is square with a side length of 300 mm. The anchor points 15 on the base are four cylinders, the radius is 5mm, and the height is 5 mm. The four centers are 10mm away from the edge of the outer contour of the base and can be connected into a square, and the side length is 280 mm. The outer contours of the transparent bottom plate 16 with the graduated scale and the grid lines and the outer contour of the fully transparent bottom plate 17 are both square, the side length is 300mm, and the thickness is 2 mm. The two

transparent bottom plates

16 and 17 are provided with holes which are matched with the positioning points 15, and the radius is 6 mm.

The base and the inserting plate of the tool are made of acrylic plates, certain plasticity is provided, experimental operation is convenient, and in addition, the tool has the characteristics of good permeability, strong bearing capacity, light self weight, easiness in cleaning and the like, so that various requirements in the using process are greatly met. The material of transparent bottom plate is the acrylic plate, can guarantee like this that the bottom plate is enough transparent, then according to the demand of shooing of difference, the bottom plate can be used separately or superpose.

The right circular groove on the front base can be used for placing a culture dish with the radius of 45 mm.

In the statistical experiment of the germination rate of the seeds and the root length of the seedlings, the invention can accurately determine the positions of the seeds or the seedlings on one hand, and can save the time of an experiment operator on the other hand, thereby being beneficial to the development of the experiment. In consideration of the fact that the data of the germination rate of the seeds and the data of the fresh weight and root length of the seedlings are the basic parts of the same experiment in the design of the experiment, the invention tool simultaneously meeting the two experiment operations is provided, and experimenters can complete the experiment contents more efficiently.

The above description is only a preferred embodiment of the present invention, and any person skilled in the art can modify the present invention or modify it into an equivalent technical solution by using the technical solution described above. Therefore, any simple modifications or equivalent substitutions made in accordance with the technical solution of the present invention are within the scope of the claims of the present invention.

Claims

1. An experimental tool for germination percentage statistics and root length statistics is characterized in that: the front surface of the tool comprises a base (1) with a hollow layer, a base hollow layer (2), a concave part (3) of the base, a first inserting plate (4) clamped in the hollow layer of the base, different seeding areas (5), (6), (7) and (8) on the first inserting plate, a second inserting plate (9) clamped in the hollow layer of the base, and different seeding areas (10), (11), (12) and (13) on the second inserting plate. The reverse side of the tool comprises a base (14), positioning points (15) on the base, a transparent bottom plate (16) with a graduated scale and grid lines and a fully transparent bottom plate (17). The outer contour of the base (1) with the hollow layer is square, and the outer contour of the base hollow layer (2) is rectangular. The concave part (3) of the base is four regular circles which are arranged at equal intervals. The outer contours of the first inserting plate (4) clamped in the hollow layer of the base and the second inserting plate (9) clamped in the hollow layer of the base are L-shaped. According to different dibbling requirements, dibbling experiments can be carried out according to different partitions on the plugboard, four different dibbling areas (5), (6), (7) and (8) are equidistantly distributed on the first plugboard and are all in a perfect circle shape, and four different dibbling areas (10), (11), (12) and (13) are equidistantly distributed on the second plugboard and are all in a perfect circle shape. The outer contour of the base (14) is square. The positioning points (15) on the base are four cylinders. The transparent bottom plate (16) with the graduated scale and the grid lines and the fully transparent bottom plate (17) are both square in outline, four holes matched with the positioning points (15) on the base are formed in the transparent bottom plate, and the holes are clamped into the cylinder for fixing when the transparent bottom plate is used. If the picture of the experimental result does not need scales, the roots can be placed on the fully transparent bottom plate (17), the transparent bottom plate (16) with the graduated scale and the grid lines is placed below the fully transparent bottom plate (17) and used for positioning and measuring the length, and the roots are moved away when the picture is taken. If the picture of the experimental result needs scale, the root can be directly placed on the (16) for taking a picture.

2. The tool as claimed in claim 1, wherein the outer contour of the base (1) with the hollow layer on the front side is square, the side length is 300mm, the outer contour of the base hollow layer (2) is rectangular with 296mm × 298mm, the thickness is 3mm, the outer contour of the concave part (3) of the base is perfect circle, the radius is 45mm, the depth is 5mm, the center is 75mm from the edge and can be connected into square, the side length is 150mm, the first insert plate (4) clamped in the base hollow layer and the second insert plate (9) clamped in the base hollow layer are L-shaped, the outer contour of the long side is rectangular with 296mm × 298mm, the thickness is 2mm, the outer contour is rectangular with 296mm × 5mm, the thickness is 7mm, the first insert plate (4) clamped in the base hollow layer are distributed with four different point regions (5), (6), (7), (8) are perfect circles, the radius is 45mm, the outer contour of the rectangular with the side is 45mm, the first insert plate (9) clamped in the base hollow layer is distributed with equal distance, the four different point regions (5), (6), (7) and the transparent bottom plate with the side length is a transparent bottom plate with the same as the side, the side length is 15mm, the transparent bottom plate is 15mm, the square, the transparent bottom plate is provided with the side with the same as the side length, the side length is 15mm, the square bottom plate with the side, the square bottom plate with the four transparent bottom plate with the side, the rectangular bottom plate with the.

3. An experimental tool for statistical tests of germination percentage and root length as claimed in claim 1, wherein: the base of the tool is made of an acrylic plate, has the characteristics of high plasticity, good permeability, strong bearing capacity, light self weight, easiness in cleaning and the like, and greatly meets various requirements in the using process. The insert plate is made of acrylic plates, four circular lines and hollow and solid round points which are used as point seed references are printed on the insert plate, and the four circular lines and the hollow and solid round points are black. The material of transparent bottom plate is the ya keli board, can guarantee like this that the bottom plate is enough transparent, according to the demand of shooing of difference, the bottom plate can superpose the use.