CN218995073U

CN218995073U - Image recognition method cell counter calibrating device

Info

Publication number: CN218995073U
Application number: CN202222907676.1U
Authority: CN
Inventors: 张珊; 贾涛; 孟卓然; 汝晶; 杜琰
Original assignee: Yunnan University of Traditional Chinese Medicine TCM
Current assignee: Yunnan University of Traditional Chinese Medicine TCM
Priority date: 2022-11-02
Filing date: 2022-11-02
Publication date: 2023-05-09
Anticipated expiration: 2032-11-02

Abstract

The utility model relates to the technical field of biomedical instrument calibration equipment, and discloses an image recognition method cell counter calibration device, which comprises a sample plate, a counting plate and a base area, wherein the sample plate is clamped at the top of the counting plate, the base area is symmetrically distributed in the counting plate in parallel, the counting plate and the base area are integrated, the base area is 10um multiplied by 10um in size, the depth of the base area is 0.2um, and a through hole is arranged in the base area and penetrates to the bottom of the counting plate. The cells on the template can be divided according to the classification of a plurality of groups of base areas, the base areas are internally provided with blank areas and areas with cell patterns, and then the corresponding base areas are subjected to image recognition according to the cell counter of the image recognition method, so that the cells in the base areas can be divided and calculated in number, regional calibration is realized, and the problem that the accuracy and precision of the conventional cell counter have larger errors, so that an accurate value cannot be obtained is solved.

Description

Image recognition method cell counter calibrating device

Technical Field

The utility model relates to the technical field of biomedical instrument calibration equipment, in particular to an image recognition method cell counter calibration device.

Background

The cytometer, as a cell metering device, requires periodic calibration to ensure the precision of the measurement. However, the calibration of the cell counter is not standard, and is usually monitored by a using unit by using home-made living cell products, and the uncertainty of the number and activity of the home-made cells over time is more important because of the limitations of the types of cells, culture conditions, survival rate, suspension concentration preparation, preservation time and the like, so that a standard biological model can not be established by using living cells for calibrating the cell counter.

At present, the cell counter needs to be manually counted by a cell counting plate method for preparing a sample for each calibration, so that the measurement accuracy of the cell counter is indirectly verified, and the errors and the deviations in a cell counter system cannot be evaluated, so that the accuracy and the precision of the existing cell counter have larger errors, and an accurate value cannot be obtained.

Disclosure of Invention

In order to overcome the above-mentioned drawbacks of the prior art, the present utility model provides an image recognition method cell counter calibration device to solve the above-mentioned problems in the prior art.

The utility model provides the following technical scheme: the utility model provides an image recognition method cell counter calibrating device, includes template, counter plate and base district, the template block is in the top of counter plate, the base district is parallel symmetrical distribution in the counter plate, the counter plate is whole with the base district, the size of base district is 10um×10um, the degree of depth of base district is 0.2um, be equipped with the through-hole in the base district, the through-hole link up to the counter plate bottom, and then the cell on the template can divide according to the classification in multiunit base district, is equipped with blank district and takes cell pattern district in the base district, carries out image recognition to the base district that corresponds according to the cell counter of image recognition method afterwards.

Further, sample areas which are distributed in parallel and symmetrically are arranged in the sample plate, the size of each sample area is 10cm multiplied by 10cm, the height of the edge of the sample plate from the sample area is 1cm, the sample plate is made of glass or highly transparent plastic, the sample plate is cleaned for three times, and then a cell sample is placed on the top of the sample plate.

Further, be equipped with the strengthening rib that is parallel symmetric distribution in the counter, and the strengthening rib degree of depth is 6um, and strengthening rib thickness is 2um, counter one side is equipped with the sign district that is parallel distribution, sign district top is the sign respectively has chinese character one to eight, the sign district corresponds every line cardinal number district, comes the cell distribution in the quick resolution template through the setting of sign district to help the naked eye resolution of inspector.

Furthermore, the image recognition method cell counter has a cytochemical staining recognition function, can realize addition, subtraction, multiplication and division four-rule operation, can calculate cytochemical staining results, and automatically calculates indexes such as total number, positive rate, integral number and the like of negative and positive reaction cells.

The utility model has the technical effects and advantages that:

1. according to the utility model, through the arrangement of the base areas, cells on the sample plate can be divided according to the classification of a plurality of groups of base areas, the base areas are internally provided with blank areas and areas with cell patterns, and then the corresponding base areas are subjected to image recognition according to the cell counter of the image recognition method, so that the cells in the base areas can be divided and calculated in number, regional calibration is realized, and the problem that the accuracy and precision of the conventional cell counter have larger errors, so that an accurate value cannot be obtained is solved.

2. According to the utility model, through the arrangement of the counting plate, in the process of counting cells, the cell distribution in the sample plate can be rapidly distinguished through the arrangement of the identification area, the naked eye distinction of a detector is facilitated, the using effect of the device is effectively improved, and the working efficiency is improved.

Drawings

Fig. 1 is a schematic diagram of the overall structure of the present utility model.

Fig. 2 is a schematic diagram of a template structure according to the present utility model.

Fig. 3 is a schematic diagram of a structure of a counting plate according to the present utility model.

Fig. 4 is a diagram illustrating a structure of a radix area according to the present utility model.

The reference numerals are: 1. a template; 2. a counting plate; 3. a base area; 101. a sample region; 201. a logo area; 301. and a through hole.

Detailed Description

The embodiments of the present utility model will be described more fully with reference to the accompanying drawings, and the configurations of the structures described in the following embodiments are merely illustrative, and the device for calibrating a cell counter for use in an image recognition method according to the present utility model is not limited to the structures described in the following embodiments, but all other embodiments obtained by a person skilled in the art without any inventive effort are within the scope of the present utility model.

Referring to fig. 1-4, the utility model provides an image recognition method cell counter calibration device, which comprises a sample plate 1, a counting plate 2 and a base area 3, wherein the sample plate 1 is clamped at the top of the counting plate 2, the base area 3 is distributed in the counting plate 2 in parallel symmetry, the counting plate 2 and the base area 3 are integrated, the base area 3 is 10um×10um in size, the depth of the base area 3 is 0.2um, a through hole 301 is arranged in the base area 3, and the through hole 301 penetrates to the bottom of the counting plate 2.

When the cell counter is used, after the template 1 is clamped at the top of the counting plate 2, cells on the template 1 are divided according to the classification of a plurality of groups of base areas 3, blank areas and cell pattern areas are arranged in the base areas 3, and then the corresponding base areas 3 are subjected to image recognition according to a cell counter pushed to a recognition method, so that the cells in the base areas 3 can be divided and calculated in number, regional calibration is realized, and the problem that an accurate value cannot be obtained due to larger errors in the accuracy and precision of the existing cell counter is solved.

The sample areas 101 are symmetrically distributed in parallel in the sample plate 1, the size of the sample areas 101 is 10cm multiplied by 10cm, the height of the edge of the sample plate 1 from the sample areas 101 is 1cm, and the sample plate 1 is made of glass or high transparent plastic.

When in use, the template 1 is cleaned three times, then a cell sample is placed on top of the template 1, and the sample area 101 is 10cm×10cm in size, so that a plurality of cells can be accommodated, the calibration efficiency is improved, the visual resolution is facilitated, and the working efficiency is further improved.

Wherein, the counter plate 2 is internally provided with reinforcing ribs which are distributed in parallel and symmetrically, the depth of the reinforcing ribs is 6um, the thickness of the reinforcing ribs is 2um, one side of the counter plate 2 is provided with a marking area 201 which is distributed in parallel, the tops of the marking areas 201 are respectively marked with Chinese characters one to eight, and the marking areas 201 correspond to each row of the base areas 3.

When in use, in the process of counting cells, the cell distribution in the template 1 can be rapidly distinguished through the arrangement of the identification area 201, and the naked eye distinction of a detector is facilitated, so that the use effect of the device is effectively improved.

The image recognition method cell counter has a cytochemical staining recognition function, and can realize addition, subtraction, multiplication and division four-rule operation, and the cytochemical staining recognition function.

When the cell chemistry staining recognition function is used, the cell chemistry staining result can be calculated, and indexes such as the total number, the positive rate, the integral number and the like of the cells in the negative and positive reactions are automatically calculated.

The working principle of the utility model is as follows:

in use, the image recognition method cell counter model number is TC20, the image recognition method cell counter is placed at a horizontal position, a power button is pressed, an instrument is started, an opening interface is displayed, then a sample plate 1 is washed three times, then a cell sample is placed on the top of the sample plate 1, the sample plate 1 is blown up and down and gently mixed, the sample area 101 is 10cm x 10cm in size, so that a plurality of cells can be accommodated, the calibration efficiency is increased, the visual distinction can be facilitated, the working efficiency is further improved, then the sample plate 1 is inserted into the image recognition method cell counter, the cells on the sample plate 1 are divided according to the classification of a plurality of groups of base areas 3, blank areas and cell pattern areas are arranged in the base areas 3, then the corresponding base areas 3 are subjected to image recognition according to the cell counter of the push-to-identification method, therefore, the cells in the base area 3 can be divided and the number calculated, the regional calibration is realized, the problem that the accuracy and the precision of the traditional cell counter have larger errors, so that an accurate value cannot be obtained is solved, when the cells are checked through a zoom mode, the focus knob can be used for adjusting an image, after the samples are counted, the focus knob is not needed to be used again, if the size of a plurality of detected samples is almost the same, the focus knob can be locked, then the focus knob can be unlocked, the image is adjusted, the center of the living cells is bright, the edge is dark, the whole cell area of dead cells is uniformly blue, no center bright point exists, so that data analysis is convenient, the counting requires 30 seconds, the number of the living cells and the number of the dead cells can be displayed on a screen, total number of cells and percent viability.

The last points to be described are: first, in the description of the present application, it should be noted that, unless otherwise specified and defined, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be mechanical or electrical, or may be a direct connection between two elements, and "upper," "lower," "left," "right," etc. are merely used to indicate relative positional relationships, which may be changed when the absolute position of the object being described is changed;

secondly: in the drawings of the disclosed embodiments, only the structures related to the embodiments of the present disclosure are referred to, and other structures can refer to the common design, so that the same embodiment and different embodiments of the present disclosure can be combined with each other under the condition of no conflict;

finally: the foregoing description of the preferred embodiments of the utility model is not intended to limit the utility model to the precise form disclosed, and any such modifications, equivalents, and alternatives falling within the spirit and principles of the utility model are intended to be included within the scope of the utility model.

Claims

1. Image recognition method cell counter calibrating device, including model (1), counting plate (2) and cardinal number district (3), its characterized in that: the sample plate (1) is clamped at the top of the counting plate (2), the base areas (3) are symmetrically distributed in the counting plate (2) in parallel, the counting plate (2) and the base areas (3) are integrated, the base areas (3) are 10um multiplied by 10um in size, and the depth of the base areas (3) is 0.2um;

and a through hole (301) is arranged in the base area (3), and the through hole (301) penetrates to the bottom of the counting plate (2).

2. The image recognition method cytometer calibration apparatus of claim 1, wherein: sample areas (101) which are distributed in parallel and symmetrically are arranged in the sample plate (1), the size of the sample areas (101) is 10cm multiplied by 10cm, and the height of the edge of the sample plate (1) from the sample areas (101) is 1cm.

3. The image recognition method cytometer calibration apparatus of claim 1, wherein: the sample plate (1) is made of glass or high-transparency plastic.

4. The image recognition method cytometer calibration apparatus of claim 1, wherein: the counting plate (2) is internally provided with reinforcing ribs which are distributed in parallel and symmetrical mode, the depth of the reinforcing ribs is 6um, and the thickness of the reinforcing ribs is 2um.

5. The image recognition method cytometer calibration apparatus of claim 1, wherein: one side of the counting plate (2) is provided with identification areas (201) distributed in parallel, chinese characters one to eight are respectively identified at the tops of the identification areas (201), and the identification areas (201) correspond to each row of base areas (3).

6. The image recognition method cytometer calibration apparatus of claim 1, wherein: the image recognition method cell counter has a cytochemical staining recognition function, and can realize addition, subtraction, multiplication and division four-rule operation.