CN104838769A - Seed germination device and method for experimental measurement - Google Patents
Seed germination device and method for experimental measurement Download PDFInfo
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- CN104838769A CN104838769A CN201510310453.9A CN201510310453A CN104838769A CN 104838769 A CN104838769 A CN 104838769A CN 201510310453 A CN201510310453 A CN 201510310453A CN 104838769 A CN104838769 A CN 104838769A
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Abstract
The invention discloses a seed germination device and method for experimental measurement. According to the seed germination device and method, a traditional germination device and method are improved. The seed germination device comprises a cylindrical bottle body and a group of glass plates which can be arranged in the bottle body, wherein each glass plate is vertically and independently arranged in the bottle body and is provided with filter paper or water absorption paper. The seed germination method comprises the following steps: firstly horizontally placing the glass plates, placing seeds on each glass plate, covering the glass plates with the filter paper or the water absorption paper which is wet by sterilization water so as to cover the seeds, pouring the sterilization water into the bottle body, vertically placing the group of glass plates with the seeds into the cylindrical bottle body, and sealing the cylindrical bottle body with a sealing film; and putting the bottle body into an incubator, and measuring various indexes at regular intervals. By virtue of the seed germination device, the upward germination of the seeds is assisted, and the counting and length measurement of the indexes can be conveniently and visually carried out.
Description
Technical field
The present invention relates to a kind of seed sprouting apparatus and method of experiment measuring.
Background technology
Seed sprouting often needs to measure germination rate, germination vigor, germination index and radicle length etc. index.And the traditional germinating method used at present is often carry out (culture dish sproutung method in culture dish, in culture dish, on chassis, plane formula is sowed seeds), need regularly to add water moisturizing in culture dish on the one hand, deposit on the other hand that radicle growth is after germination curling, seed displacement and the problem such as winding mutually, make the measurement of each index there is certain difficulty.
Summary of the invention
Main purpose of the present invention is to provide a kind of seed sprouting device of experiment measuring, utilizes it to contribute to the upright germination of seed, can more convenient, counting and the linear measure longimetry of carrying out each index intuitively.
For achieving the above object, the present invention takes following design:
A seed sprouting device for experiment measuring, it includes: a cylindricality bottle; Can insert the glass plate in bottle for one group, each glass plate independent vertical is placed in bottle; Every block glass plate is equipped with a filter paper or blotting paper.
In the seed sprouting device of described experiment measuring, be also equipped with a slot board, be placed in the bottom in bottle, this slot board has one group of slot inserted for glass plate.
In the seed sprouting device of described experiment measuring, described one group of glass plate is three pieces of rectangular glass, and each rectangular glass dual-side of inserting in bottle is all against on the inwall of bottle, and the base of three pieces of glass plates composition triangle.
In the seed sprouting device of described experiment measuring, each glass plate has and laterally holds in the palm rib, this is holder rib distance base 4 ~ 6cm laterally.
In the seed sprouting device of described experiment measuring, described transverse direction holder rib thickness is not more than 2mm.
In the seed sprouting device of described experiment measuring, each glass plate has mark facility.
Another object of the present invention is to provide a kind of seed germinating method of experiment measuring, utilizes the method can carry out counting and the linear measure longimetry of each index intuitively, can overcome the deficiency of traditional germinating method.
For achieving the above object, the present invention takes following design:
A seed germinating method for experiment measuring, comprises any one seed sprouting device above-mentioned; Its method is as follows:
A) one group of glass plate is kept flat, seed to be put respectively on each glass plate and apart from 4-6cm place, its base;
B) be covered in respectively on glass plate to cover seed after each filter paper or blotting paper aqua sterilisa being soaked;
C) in bottle, pour aqua sterilisa into, the height of aqua sterilisa is 2 ~ 4cm, is then vertically put in respectively in cylindricality bottle by seed-bearing for the band got ready one group of glass plate;
D) with sealed membrane, cylindricality bottle is sealed; Again this cylindricality bottle is put into incubator.
E) periodic measurement indices.
In the seed germinating method of described experiment measuring, described step b) in, the region that filter paper or blotting paper are covered on glass plate is: above seed, at least begin in 2cm place, and the base to glass plate is stopped.
In the seed germinating method of described experiment measuring, described step c) in, one group of glass plate be vertically put in cylindricality bottle adopts three pieces, and to enclose triangle be good.
In the seed germinating method of described experiment measuring, described step c) in, before pour aqua sterilisa in bottle, first put into slot board, then respectively seed-bearing for the band got ready each glass plate put into bottle and insert slot corresponding on slot board.
Advantage of the present invention is:
1) impel the upright germination of seed, be not easily mutually wound around, be beneficial to more convenient, to carry out each index intuitively counting and linear measure longimetry;
2) seed being positioned at the upright germination of bottle is more conducive to growth, and does not need the moisturizing that regularly adds water, and namely can continue the supply meeting seed sprouting moisture, percentage of seedgermination is higher, and growth rate is faster;
3) adopt three pieces of rectangular glass to be vertically put in cylindricality bottle, and enclose triangle, be beneficial between glass plate and mutually rely on, stablize with holding position.
Accompanying drawing explanation
Fig. 1 is seed sprouting device one preferred embodiment structural representation (not adding aqua sterilisa) of experiment measuring of the present invention.
Fig. 2 is another example structure schematic diagram of seed sprouting device of experiment measuring of the present invention.
Fig. 3 is an example structure schematic diagram of glass plate of the present invention.
Fig. 4 is slot board one example structure schematic diagram of the present invention.
Fig. 5 is another example structure schematic diagram of slot board of the present invention.
Fig. 6 is the step view in the inventive method.
In figure: 1-bottle; 2-glass plate; 201-laterally holds in the palm rib; 202-marks facility; 3-filter paper; 4-slot board; 401-groups of slots; 401 '-slot; 10-chitting piece; 10 '-seed.
Below in conjunction with drawings and the specific embodiments, the present invention is described in further details.
Embodiment
Consult shown in Fig. 1, the seed sprouting device of experiment measuring of the present invention mainly includes bottle 1, one group of glass plate 2 and filter paper 3 (or blotting paper).
Described bottle 1, in cylindricality, can be the special bottle of special experiment, also daily bottle, and meeting is column.
The described one group glass plate can inserted in bottle generally adopts the more convenient use of rectangle.Each glass plate independent vertical is placed in bottle, and its length is not limit, and facilitates hand-held (put into or shift out).
Every block glass plate 2 is supporting is provided with filter paper 3 or blotting paper, and the mechanism of action of filter paper 3 or blotting paper is prior art, does not repeat herein.
As depicted in figs. 1 and 2, three glass plates be one group more practical, when three glass plates upright insert in bottle time, each rectangular glass dual-side is all against on the inwall of bottle, and three pieces of glass plates can rely on mutually (enclosing triangle), can better maintain upright state.
Certainly, also further can arrange a slot board 4, this slot board having one group of slot for glass plate insertion (can be composition triangle as shown in Figure 4 or polygonal groups of slots 401; Or the in line slot 401 ' set up as shown in Figure 5, can be many rows, the size depending on bottle diameter), first slot board 4 can be placed in the bottom in bottle, then glass plate be put into bottle respectively and is placed in the slot of correspondence position.Such design, slot board 4 can play the effect of auxiliary positioning.
Also can establish horizontal holder rib 201 further on each glass plate 4, its thickness is not more than 2mm, and this laterally at 4 ~ 6cm, should be assisted and hold seed, avoided its downward landing in water on holder rib distance base.
See Fig. 3, each glass plate also can be provided with mark facility 202, can be protruding sticker and the scale that formed, or be directly the rule of printing or etching, facilitate staff to observe intuitively.
The seed position of putting on each glass plate is also independent design of the present invention, seed should be put apart from 4-6cm place, glass plate base; The selected reason of this distance is: in germination process, and seed invests on the glass plate of uprightly inserting in bottle through the covering plaster of filter paper, and now, as apart from too low, seed is easily fallen in water, and easy rotten kind, apart from too high, water absorption is few, and speed of germination is slow.
The filter paper covered or blotting paper will be tried one's best large area, and ensure the supply that institute absorb water point first, second the enough towing force of guarantee generation hold seed, make seed be not easy to fall in water as far as possible.Therefore the filter paper covered or blotting paper at least will cover dash area region as shown in Figure 6 (top M is at least 2cm, and bottom is paved with as good).
In the present invention program, if select the cylindricality bottle that height is 10-12cm, diameter is 6-8cm, then the glass plate length of three pieces of rectangles is 10-14cm, base width is 4-5cm, filter paper (or blotting paper) thickness is 0.01-0.15cm, aqua sterilisa 50-100ml, seed diameter 0.01-0.15cm is advisable.
Below with one preferably specific embodiment the present invention will be further described:
Select cylindricality bottle, height is 12cm, and diameter is 8cm;
The glass plate of three pieces of rectangles, every block glass plate length is 12cm, and base width is 5cm;
Filter paper thickness is 0.15cm, is cut to identical with glass plate size;
Aqua sterilisa 100ml;
Seed diameter 0.15cm.
Concrete grammar is:
Three pieces of glass plates are kept flat, respectively seed is put in distance 5cm position, base (shown in Figure 6);
Be covered on glass plate after filter paper aqua sterilisa is wetting;
Pour aqua sterilisa 100ml in bottle, the glass plate then three pieces being well placed seed is vertically put in bottle respectively, and making three pieces of glass plates mutually enclose cross section is triangular shaped (base composition equilateral triangle);
Bottle described in upper step is put into incubator, carries out arranging the cultivation such as illumination, temperature according to seed characteristics and testing index;
Every day measures indices.
Here is with culture dish sproutung method for contrast, to 3 alfalfa variety application sheet glass sproutung method tests, within 4 days, adds up the germination rate of clover, root length and root/shoot ratio afterwards, to providing basis for the improvement of germinating method.
Different cultivars and germinating apparatus are on the impact of clover germination rate
Different cultivars and germinating apparatus are on the impact of clover root length
Different cultivars and germinating apparatus are on the impact of clover root/shoot ratio
Can be illustrated by above-mentioned experiment:
All there is significant difference (probable value p<0.05) compared with the control in three alfalfa variety germination rates, root length and root/shoot ratios.Wherein germination rate improves 24.5% ~ 98.7% than contrast, and root length and root/shoot ratio add 97.18% ~ 393.33% and 14.24% ~ 71.06% than contrasting respectively.Illustrate that this germinating apparatus and experimental technique thereof can effectively improve the indexs such as the germination rate of plant.
The various embodiments described above can not to depart under protection scope of the present invention in addition some changes, thus above explanation to comprise and the structure shown in accompanying drawing should be considered as exemplary, and be not used to the protection domain limiting the present patent application patent.
Claims (10)
1. a seed sprouting device for experiment measuring, is characterized in that including:
A cylindricality bottle;
Can insert the glass plate in bottle for one group, each glass plate independent vertical is placed in bottle;
Every block glass plate is equipped with a filter paper or blotting paper.
2. the seed sprouting device of experiment measuring as claimed in claim 1, is characterized in that: be also equipped with a slot board, be placed in the bottom in bottle, this slot board has one group of slot inserted for glass plate.
3. the seed sprouting device of experiment measuring as claimed in claim 1 or 2, it is characterized in that: described one group of glass plate is three pieces of rectangular glass, each rectangular glass dual-side of inserting in bottle is all against on the inwall of bottle, and three pieces of glass plates surround triangle.
4. the seed sprouting device of experiment measuring according to claim 1 and 2, is characterized in that: have on each glass plate and laterally hold in the palm rib, and this is holder rib distance base 4 ~ 6cm laterally.
5. the seed sprouting device of experiment measuring according to claim 4, is characterized in that: described transverse direction holder rib thickness is not more than 2mm.
6. the seed sprouting device of experiment measuring according to claim 1 and 2, is characterized in that: on each glass plate, have mark facility.
7. a seed germinating method for experiment measuring, to comprise in claim 1 to 6 the seed sprouting device of any one; It is characterized in that method is as follows:
A) one group of glass plate is kept flat, seed to be put respectively on each glass plate and apart from 4-6cm place, its base;
B) be covered in respectively on glass plate to cover seed after each filter paper or blotting paper aqua sterilisa being soaked;
C) in bottle, pour aqua sterilisa into, the height of water is 2 ~ 4cm, is then vertically put in respectively in cylindricality bottle by seed-bearing for the band got ready one group of glass plate;
D) with sealed membrane, cylindricality bottle is sealed; Again this cylindricality bottle is put into incubator.
E) periodic measurement indices.
8. the seed germinating method of experiment measuring according to claim 7, is characterized in that: described step b) in, filter paper or the blotting paper region be covered on glass plate is that at least 2cm place begins to stop to the base of glass plate above seed.
9. the seed germinating method of experiment measuring according to claim 7, it is characterized in that: described step c) in, one group of glass plate be vertically put in cylindricality bottle adopts three pieces of rectangular glass, each rectangular glass dual-side of inserting in bottle is all against on the inwall of bottle, and three pieces of glass plates enclose triangle.
10. the seed germinating method of the experiment measuring according to claim 7,8 or 9, it is characterized in that: described step c) in, before pour aqua sterilisa in bottle, first put into slot board, then respectively seed-bearing for the band got ready each glass plate put into bottle and insert slot corresponding on slot board.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510310453.9A CN104838769A (en) | 2015-06-09 | 2015-06-09 | Seed germination device and method for experimental measurement |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510310453.9A CN104838769A (en) | 2015-06-09 | 2015-06-09 | Seed germination device and method for experimental measurement |
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| CN104838769A true CN104838769A (en) | 2015-08-19 |
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| CN201510310453.9A Pending CN104838769A (en) | 2015-06-09 | 2015-06-09 | Seed germination device and method for experimental measurement |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111357425A (en) * | 2018-12-25 | 2020-07-03 | 东北林业大学 | Experimental tool for germination percentage statistics and root length statistics |
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| CN201303492Y (en) * | 2008-06-03 | 2009-09-09 | 东北林业大学 | Vertical-board gemmation box |
| CN201797718U (en) * | 2010-04-19 | 2011-04-20 | 东北农业大学 | Seed germination tester |
| CN202514273U (en) * | 2012-03-13 | 2012-11-07 | 镇江恒欣肥料科技有限公司 | Root length measuring device for seed germination index experiment |
| CN104094695A (en) * | 2013-04-02 | 2014-10-15 | 镇江瑞繁农艺有限公司 | Cabbage seed germination rate detection device and detection method |
| CN104137678A (en) * | 2014-08-01 | 2014-11-12 | 贵州大学 | Seed vertical germination observation device |
| CN204244672U (en) * | 2014-11-21 | 2015-04-08 | 玉溪中烟种子有限责任公司 | Seed paper folding germinating bed |
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2015
- 2015-06-09 CN CN201510310453.9A patent/CN104838769A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201303492Y (en) * | 2008-06-03 | 2009-09-09 | 东北林业大学 | Vertical-board gemmation box |
| CN201797718U (en) * | 2010-04-19 | 2011-04-20 | 东北农业大学 | Seed germination tester |
| CN202514273U (en) * | 2012-03-13 | 2012-11-07 | 镇江恒欣肥料科技有限公司 | Root length measuring device for seed germination index experiment |
| CN104094695A (en) * | 2013-04-02 | 2014-10-15 | 镇江瑞繁农艺有限公司 | Cabbage seed germination rate detection device and detection method |
| CN104137678A (en) * | 2014-08-01 | 2014-11-12 | 贵州大学 | Seed vertical germination observation device |
| CN204244672U (en) * | 2014-11-21 | 2015-04-08 | 玉溪中烟种子有限责任公司 | Seed paper folding germinating bed |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111357425A (en) * | 2018-12-25 | 2020-07-03 | 东北林业大学 | Experimental tool for germination percentage statistics and root length statistics |
| CN111357425B (en) * | 2018-12-25 | 2024-04-30 | 东北林业大学 | Experimental tool for germination rate statistics and root length statistics |
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Application publication date: 20150819 |