CN110865080A - Seed disk capable of collecting soybean hilum phenotype image data and collection method thereof - Google Patents

Seed disk capable of collecting soybean hilum phenotype image data and collection method thereof Download PDF

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Publication number
CN110865080A
CN110865080A CN201911310763.5A CN201911310763A CN110865080A CN 110865080 A CN110865080 A CN 110865080A CN 201911310763 A CN201911310763 A CN 201911310763A CN 110865080 A CN110865080 A CN 110865080A
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seed
placing
seeds
phenotype
area
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Inventor
冯献忠
贺红
于慧
曲美霞
冷建田
李素梅
袁德明
王梓森
于翀宇
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Shandong University
Northeast Institute of Geography and Agroecology of CAS
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Shandong University
Northeast Institute of Geography and Agroecology of CAS
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84Systems specially adapted for particular applications
    • GPHYSICS
    • G06COMPUTING; CALCULATING OR COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00Image analysis
    • G06T7/90Determination of colour characteristics
    • HELECTRICITY
    • H04ELECTRIC COMMUNICATION TECHNIQUE
    • H04LTRANSMISSION OF DIGITAL INFORMATION, e.g. TELEGRAPHIC COMMUNICATION
    • H04L67/00Network arrangements or protocols for supporting network services or applications
    • H04L67/01Protocols
    • H04L67/10Protocols in which an application is distributed across nodes in the network

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  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Signal Processing (AREA)
  • General Health & Medical Sciences (AREA)
  • Computer Networks & Wireless Communication (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Theoretical Computer Science (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

The seed disk capable of collecting soybean hilum phenotype image data comprises a base and a dial plate which are integrally connected with each other, wherein the dial plate comprises a seed placing area for placing seeds, and the seed placing area comprises a placing groove; the placing groove comprises a circular groove and a rectangular groove, the rectangular groove is arranged at the bottom in the circular groove, the height of the circular groove is larger than that of the rectangular groove, and the height of the circular groove is smaller than that of the seeds. The dial plate comprises a verification area and an information placing area, wherein a colorimetric card is placed in the verification area and is used for carrying out color verification on a final image of the seeds placed on the seed placing area; the information placing area is used for placing the seed coding information of the current shooting batch and other related texts.

Description

Seed disk capable of collecting soybean hilum phenotype image data and collection method thereof
Technical Field
The invention relates to the field of agricultural science research, in particular to a fruiting disc capable of collecting soybean hilum phenotype image data and a collection method thereof.
Background
The hilum of soybean refers to the scar left after the seed falls off from the seed stalk or placenta, and has a circular, elliptical, oval shape, etc. The hilum is the only pathway for plants to deliver nutrients to the seed during seed formation.
The color, shape, length, width, concave-convex and existing parts of the soybean hilum are different according to different plant species, and can be used as an important basis for identifying seeds.
In order to realize artificial intelligence breeding, a large amount of seeds need to be subjected to phenotype data acquisition in the seed test process, wherein the phenotype data acquisition comprises the acquisition of hilum data. At present, the navel data acquisition process is completely finished manually, single-particle real navel is shot manually at multiple angles, manual real-particle image acquisition is carried out to acquire navel images of the navel, and then the navel data acquisition process is processed by a computer to identify phenotype data of the navel.
However, the above method has the following disadvantages:
(1) the image acquisition of the single-particle seed umbilicus is carried out manually, the workload is large, and the method is not suitable for acquiring the phenotype data of the seed umbilicus in batches;
(2) for the collection of a large number of seed images, the overall processing flow has low efficiency, long time consumption and low image collection efficiency;
(3) the specifications of the shot plant images are different, such as size, position and the like.
Disclosure of Invention
The invention aims to solve the problem of making up the defects of the prior art and provides a seed disk capable of acquiring soybean hilum phenotype image data and an acquisition method thereof, wherein the seed disk can acquire soybean hilum phenotype data in batches and prevent inaccurate acquired data caused by rolling of soybean seeds.
A seed disk capable of collecting soybean hilum phenotype image data comprises a base and a dial plate which are integrally connected with each other, wherein the dial plate comprises a seed placing area for placing seeds, and the seed placing area comprises a placing groove;
the placing groove comprises a circular groove and a rectangular groove, the rectangular groove is arranged at the bottom in the circular groove, the height of the circular groove is larger than that of the rectangular groove, and the height of the circular groove is smaller than that of the seeds.
Further, the number of the placing grooves is n × m, and the seed placing area is always rectangular.
Further, the rectangular groove comprises a wide part, a long part and a second high part, wherein the wide part is smaller than the width of the minimum seeds of the collection batch, the long part is larger than the length of the maximum seeds of the collection batch, and the second high part is not more than half of the height of the seeds of the collection batch.
Further, the circular groove includes a first high portion having a diameter of 11.0mm, a height of 5.0mm, a length of the wide portion of the rectangular groove is 6.3mm, a length of the long portion is 9.0mm, and a height of 3.5 mm.
Further, the dial plate comprises a verification area and an information placing area, a colorimetric card is placed in the verification area and used for conducting color verification on a final image of the seeds placed on the seed placing area, and the information placing area is used for placing the seed code information of the current shooting batch and other related texts.
Further, row and column marks are arranged on the transverse side edge and the longitudinal side edge of the seed placing area.
Further, the base comprises handheld holes, and the handheld holes are concavely arranged on the four frames of the base.
Further, the base includes the gusset plate, the gusset plate set up in the base bottom is used for strengthening the intensity of base.
A method for acquiring phenotype data by using the daughter disc capable of acquiring soybean hilum phenotype image data, comprising the following steps:
step1, placing a color comparison card in a verification area of the sub-real disc, and placing a two-dimensional code in an information placing area;
step2, placing seeds to be tested on the placing grooves of the seed solid discs, and poking the seeds by using tweezers to enable the hilum of the seeds to be placed upwards;
step3, placing the sub real disc at a specified position, placing a camera device and a light source above the sub real disc, and taking a picture of the sub real disc by adopting the camera device and storing the taken picture;
step4, taking a batch of seeds of the seeds to be tested, if the number of the soybean variety is not changed, turning to Step2 to continue repeating the steps, and if the number of the soybean variety is changed, turning to Step1 to continue repeating the steps;
step5, transmitting the image files of the plurality of shot seeds to a designated cloud server;
step6, calling an image processing program by the cloud server to process the image file in parallel, extracting the phenotype characteristic data of the seeds, the color comparison card information of the verification area and the two-dimensional code type number of the information placement area, and storing the phenotype characteristic data, the color comparison card information of the verification area and the two-dimensional code type number in a phenotype database;
and Step7, if the seeds of the seeds to be tested still exist, turning to Step1 to continue repeating the steps, and if not, finishing the acquisition of the soybean hilum phenotype image data.
Further, after the step of Step4 and before the step of Step5, the method comprises the following steps:
and step41, placing a pure white color comparison card in front of the camera equipment, completely covering the seeds on the seed disk, photographing the color card, and then taking away the color card.
Compared with the prior art, the invention has the following beneficial effects:
the invention provides a seed disk special for acquiring soybean hilum phenotype image data, which realizes phenotype image data acquisition of batch soybean seeds, wherein the phenotype image data acquisition comprises phenotype data of hilum and kidney of soybean. And then, carrying out Computer Vision (CV) processing on the acquired images, and establishing a model through deep learning to extract features so as to acquire soybean hilum phenotype image data for big data analysis. The son real disc provided by the invention can greatly improve the acquisition efficiency and data accuracy of soybean hilum phenotype image data.
According to the seed solid disc capable of acquiring soybean hilum phenotype image data, the seed solid disc with the rectangular groove is arranged at the bottom of the seed solid disc to replace a common seed solid disc, the hilum of the seed can be upward through proper adjustment after the seed is placed in the seed solid disc, the rectangular groove at the bottom enables the seed to be stably embedded in the disc, the hilum of the seed is always kept upward, batch image acquisition is facilitated, and the image acquisition speed is accelerated.
The seed disk capable of acquiring the soybean hilum phenotype image data can position the light source and the camera equipment when the phenotype image data of seeds are acquired in batches, and the accuracy of a normalization algorithm in the soybean image processing of a later-stage computer is ensured.
Drawings
Fig. 1 is a schematic overall structure diagram of a sub-solid disc according to embodiment 1 of the present invention;
fig. 2 is a schematic bottom structure view of a chassis according to embodiment 1 of the present invention;
FIG. 3 is an enlarged view of the structure of a placing groove of a seed placing region in example 1 of the present invention;
fig. 4 is a sectional view of a placing groove of a seed placing area according to example 1 of the present invention;
fig. 5 is a schematic overall structure diagram of a sub-solid disc according to embodiment 2 of the present invention;
fig. 6 is a schematic view of the entire structure of the sub-solid disc in embodiment 3 of the present invention.
Reference numbers in the figures:
1 sub-solid dish, 11 bases, 111 reinforcing plates, 1111 transverse and longitudinal reinforcing plates, 11111 reinforcing ribs, 1112X-shaped reinforcing plates, 112 hollowed-out grooves, 113 hand-held holes, 12 dials, 121 seed placement areas, 1211 row and column marks, 1212 placement grooves, 12121 circular grooves, 121211 first high parts, 12122 rectangular grooves, 121221 wide parts, 121222 long parts, 121223 second high parts, 122 verification areas and 123 information placement areas.
Detailed Description
The present invention will be further described below based on preferred embodiments with reference to the accompanying drawings.
In addition, the various components on the drawings are enlarged (thick) or reduced (thin) for convenience of understanding, but this is not intended to limit the scope of the present invention.
Singular references also include plural references and vice versa.
In the description of the embodiments of the present invention, it should be noted that, if the terms "upper", "lower", "left", "right", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings or orientations or positional relationships that are usually placed when the products of the present invention are used, the orientations or positional relationships are only used for convenience of describing the present invention and simplifying the description, but the terms do not indicate or imply that the devices or elements to be referred to must have specific orientations, be constructed in specific orientations, and operate, and thus, the present invention should not be construed as being limited. Furthermore, the terms first, second, etc. may be used in the description to distinguish between different elements, but these should not be limited by the order of manufacture or by importance to be understood as indicating or implying any particular importance, and their names may differ between the detailed description of the invention and the claims.
The terminology used in the description is for the purpose of describing the embodiments of the invention and is not intended to be limiting of the invention. It is also to be understood that, unless otherwise expressly stated or limited, the terms "disposed," "connected," and "connected" are intended to be open-ended, i.e., may be fixedly connected, detachably connected, or integrally connected; they may be mechanically coupled, directly coupled, indirectly coupled through intervening media, or may be interconnected between two elements. Those skilled in the art will specifically understand that the above description is intended to be within the meaning of the present invention.
The invention provides a seed disk capable of acquiring soybean hilum phenotype image data, wherein a batch of seeds are placed in the seed disk, the hilum is arranged upwards, then the seeds are photographed so as to acquire soybean phenotype image data, wherein the soybean phenotype image data comprises soybean hilum and kidney isophenotype data, then Computer Vision (CV) processing is carried out on the acquired images, and characteristics are extracted by establishing a model through deep learning so as to acquire the soybean hilum phenotype image data for big data analysis.
Example 1
Referring to fig. 1 to 4, the present invention provides a sub-real disk capable of collecting soybean umbilical phenotype image data, including a sub-real disk 1, where the sub-real disk 1 is square, the sub-real disk 1 includes a base 11 and a dial 12, and the base 11 and the dial 12 are fixedly connected to each other. Preferably, in the present embodiment, the base 11 and the dial 12 are integrally connected.
The base 11 includes gusset plate 111 and fretwork groove 112, and gusset plate 111 is equipped with a plurality of, and all sets up in base 11 bottom to cut apart into a plurality of fretwork grooves 112 base 11 bottom, when making dial plate 12 fixed connection on base 11, reducible sub real dish 1 inside material lightens the weight of sub real dish 1.
Specifically, the reinforcing plate 111 includes a horizontal reinforcing plate 1111 and an X-shaped reinforcing plate 1112, the horizontal reinforcing plate 1111 is disposed in the horizontal direction and the vertical direction of the bottom of the base 11, the X-shaped reinforcing plate 1112 is disposed in the X-shape of the bottom of the base 11, and both the horizontal reinforcing plate 1111 and the X-shaped reinforcing plate 1112 can reinforce the strength of the base 11 and prevent the base 11 from being deformed by stretching.
Preferably, horizontal reinforcement plate 1111 includes reinforcing rib 11111, and reinforcing rib 11111 sets up the edge that is close to base 11 on horizontal reinforcement plate 1111, and reinforcing rib 11111's height is greater than horizontal reinforcement plate 1111's height, and is the echelonment structure between reinforcing rib 11111 and the horizontal reinforcement plate 1111, and reinforcing rib 11111 can further strengthen base 11's intensity, prevents base 11 tensile deformation.
The base 11 further comprises a plurality of handheld holes 113, and the handheld holes 113 are concavely arranged on four side frames of the base 11, so that the sub solid disc 1 can be taken.
The dial 12 includes a seed placement area 121, a verification area 122, and an information placement area 123.
The seed placement area 121 is used for placing a batch of seeds for shooting.
A color comparison card is placed in the verification area 122 for performing color verification on the final image of the seeds placed on the seed placement area 121, and the verification area 121 is fixed in position so as to improve the image processing speed, and is one of the elements of image acquisition standardization.
The information placing area 123 is used for placing the seed code information of the current shooting batch and other related texts, specifically, the information placing area 123 can be used for placing a two-dimensional code or a seed code label, preferably, the information placing area 123 is used for placing a two-dimensional code, the two-dimensional code has the advantages that the two-dimensional code can replace the traditional code text, the placing position and the shooting angle are not considered, the image processing speed can be improved, and the arrangement is also one of the elements of image acquisition standardization.
The seed placing area 121 is rectangular, and includes a plurality of placing grooves 1212 capable of placing seeds therein, the placing grooves 1212 are recessed toward the base 11, the placing grooves 1212 are used for placing batches of seeds, and the seeds can directly fall into the placing grooves 1212.
The number of the placing grooves 1212 is n × m, that is, the number in the transverse direction may be the same as or different from that in the longitudinal direction, but the seed placing region 121 is always rectangular in n × m shape. Preferably, in this embodiment, two sizes of the placing grooves 1212 are provided, 12 × 12 and 12 × 8, respectively, that is, 144 and 96, respectively, in consideration of the number of seeds on one soybean plant.
In this embodiment, in the shooting range, the distance between two adjacent placing grooves 1212 is 13.1 mm.
The placing grooves 1212 provided by the invention can be set to have different sizes according to actual needs, the hole sites of the placing grooves 1212 can be enlarged when the shot seed particles are larger, and the hole sites of the placing grooves 1212 can be reduced when the shot seed particles are smaller. The soybean shooting device is mainly used for shooting soybean seeds in the embodiment, so the diameter of the hole site of the placing groove 1212 is not more than 11.0mm, and the placing groove 1212 is sunken downwards, so that the soybean can directly fall into the placing groove 1212 and cannot be easily separated.
The placing groove 1212 includes a circular groove 12121 and a rectangular groove 12122, the rectangular groove 12122 is provided at the bottom in the circular groove 12121, the lower half portion for clamping the seed, the circular groove 12121 includes a first high portion 121211, the rectangular groove 12122 includes a second high portion 121223, the height of the circular groove 12121 is greater than the height of the rectangular groove 12122, i.e., the height of the first high portion 121211 is greater than the height of the second high portion 121223, the seed is prevented from falling out when the seed real disk 1 is moved, and at the same time, the height of the circular groove 12121 is not greater than the height of the seed, i.e., the height of the first high portion 121211 is not greater than the height of the seed, thereby avoiding shadow generation when the seed is photographed and affecting subsequent computer recognition processing.
The length, width and height dimensions of the rectangular slot 12122 can be flexibly adjusted to the size of the seed to be photographed. Specifically, the rectangular groove 12122 includes a wide portion 121221, a long portion 121222 and a second high portion 121223, wherein the wide portion 121221 should be slightly smaller than the width of the smallest seed of the collection batch, so that the seed can be placed in the rectangular groove 12122 to be clamped and prevented from rolling, the long portion 121222 should be larger than the length of the largest seed of the collection batch, so that the seed angle can be adjusted by using tweezers, the seed is kept with the hilum facing upward, the second high portion 121223 is generally not more than half of the height of the seed of the collection batch, so that the widest portion of the seed is exposed at the upper part of the rectangular groove, the complete overhead view image of the seed can be collected, and various phenotype data such as the length and the width of the seed can be extracted at the same time.
The sub-solid disc 1 in this embodiment is suitable for photographing a seed of a larger size, in which the diameter of the circular groove 12121 is 11.0mm and the height of the first high portion 121211 is 5.0 mm. The wide portion 121221 of rectangular slot 12122 has a length dimension of 6.3mm, the long portion 121222 has a length dimension of 9.0mm, and the second high portion 121223 has a height dimension of 3.5 mm.
The lateral and longitudinal sides of the seed placement area 121 are provided with row and column labels 1211, corresponding to the column coordinates and the row coordinates, which mark each seed in the seed placement area 121 for finding the corresponding seed according to the coordinates in the subsequent computer identification.
Wherein the column coordinates of the row and column labels 1211 are labeled 1, 2, 3, … …, and the row coordinates of the row and column labels 1211 are labeled A, B, C, … …, in terms of the size of the daughter solid tray and the number of seeds of a soybean plant to be harvested, in this embodiment, two sizes of placement slots 1212 are provided, which are 12 × 12 and 12 × 8 sizes of daughter solid trays, respectively.
The sub-real disc 1 is made of non-reflective materials, preferably, in the embodiment, the sub-real disc 1 is made of white or black industrial nylon as a raw material and is manufactured in a 3D printing mode, poor effects such as light reflection and the like are avoided when the seed placing area 121 is shot, and meanwhile, the whole stability of the sub-real disc 1 can be guaranteed due to the fact that the surface of the industrial nylon is not reflective and is not prone to deformation.
Example 2
The structure of the sub-solid disc 1 in this embodiment is substantially the same as that in embodiment 1, except that: the sub-seed disk 1 in this embodiment is suitable for shooting medium-sized seeds, and the plurality of placing grooves 1212 are reduced in size, wherein the diameter of the circular groove 12121 is 10.04mm, the height of the first high portion 121211 is 5.0mm, the length of the wide portion 121221 of the rectangular groove 12122 is 5.3mm, the length of the long portion 121222 is 8.0mm, and the height of the second high portion 121223 is 3.5 mm.
Example 3
The structure of the sub-solid disc 1 in this embodiment is substantially the same as that in embodiment 1, except that: the sub-solid disc 1 in this embodiment is suitable for photographing seeds of a smaller size, and the plurality of placing grooves 1212 are reduced in size, wherein the diameter of the circular groove 12121 is 8.5mm, the height of the first high portion 121211 is 3.5mm, the length of the wide portion 121221 of the rectangular groove 12122 is 4.0mm, the length of the long portion 121222 is 6.5mm, and the height of the second high portion 121223 is 2.5 mm.
The following describes a method for acquiring phenotypic data using the daughter disc capable of acquiring soybean hilum phenotypic image data as described above.
The method comprises the following steps:
step1, placing a color comparison card in the verification area 122 of the sub-solid disc 1, and placing a two-dimensional code in the information placing area 123.
And Step2, placing the seeds to be tested on the placing grooves 1212 of the seed dish 1, and using tweezers to stir the seeds to enable the hilum of the seeds to be placed upwards.
Wherein, the seeds placed on the seed dish 1 are generally placed continuously, so that the condition of lacking seeds does not occur in the middle, and preferably, the seeds with full and round color should be used as much as possible.
And step3, placing the sub-real disk 1 at a specified position, placing a camera device and a light source above the sub-real disk 1, and taking a picture of the sub-real disk 1 by using the camera device and storing the taken picture.
Preferably, the camera device is a high-pixel camera device and is provided with a shadowless lamp, and the camera device can also be used for shooting by a mobile phone, wherein the height of the mobile phone is fixed during shooting, and the lens is positioned right above the symmetric center of the sub-solid disc.
Preferably, when the real disc 1 is photographed, the photographing should be performed in a light-proof room, so that the light source is not changed in the whole photographing process; meanwhile, the light source is a fixed light source and should be positioned right above or close to the right above the symmetrical center of the sub real disk 1, so that obvious shadows on the sub real disk 1 are avoided; in addition, the light source is prevented from being shielded during photographing, and the condition of brightness change is prevented.
And Step4, taking the next batch of seeds of the seeds to be tested, if the number of the soybean variety is not changed, turning to Step2 to continue repeating the steps, and if the number of the soybean variety is changed, turning to Step1 to continue repeating the steps.
It should be noted that when the above steps are repeated, the relative positions of the sub-real disk 1, the image pickup device and the light source should be kept unchanged, so that the color homogenization difference value of each point of the shot picture is kept unchanged.
If the position of the light source is changed, the following steps need to be executed, and if the light source is not changed in the whole shooting process, the following steps are executed only once:
step41, a pure white color comparison card is placed in front of the camera equipment, the seeds on the seed solid disc 1 are completely covered, the color comparison card is photographed, and then the color comparison card is taken away.
Preferably, 255 and 255 standard colorimetric cards can be used.
The Step41 is to initialize the color homogenization difference value of the sub-real disk 1, which can avoid the situation of the difference of the seed surface color caused by the oversize seed placing area 121, the dispersed seed position and the different distance from the camera equipment, and is convenient for the computer image processing algorithm to obtain the accurate seed surface color and glossiness.
And step5, transmitting the image files of the plurality of photographed seeds to a designated cloud server.
Step6, the cloud server calls an image processing program to process the image file in parallel, extracts the phenotype characteristic data of the seeds, the color comparison card information of the verification area 122 and the two-dimensional code type number of the information placing area 123, and stores the phenotype characteristic data, the color comparison card information and the two-dimensional code type number in a phenotype database.
And Step7, if the seeds of the seeds to be tested still exist, turning to Step1 to continue repeating the steps, and if not, finishing the acquisition of the soybean hilum phenotype image data.
While the above detailed description of the embodiments of the present invention has been described, it will be apparent to those skilled in the art that modifications and improvements can be made without departing from the spirit of the invention, for example, the dimensions of the circular and rectangular grooves can be modified according to the size of the seed to be photographed, and such modifications and improvements are also within the scope of the appended claims.

Claims (10)

1. A kind of son real disc that can gather the phenotype image data of soybean hilum, characterized by that:
the son solid disc (1) comprises a base (11) and a dial (12) which are integrally connected with each other, the dial (12) comprises a seed placing area (121) for placing seeds, and the seed placing area (121) comprises a placing groove (1212);
the placement groove (1212) includes a circular groove (12121) and a rectangular groove (12122), the rectangular groove (12122) being provided at the bottom inside the circular groove (12121), the height of the circular groove (12121) being greater than the height of the rectangular groove (12122), and the height of the circular groove (12121) being less than the height of the seeds.
2. The seed disk capable of acquiring soybean hilum phenotype image data according to claim 1, wherein the number of the placing grooves (1212) is n x m, and the seed placing area (121) is always rectangular.
3. The daughter disc of claim 1, wherein said rectangular groove (12122) comprises a wide portion (121221), a long portion (121222) and a second high portion (121223), said wide portion (121221) being smaller than the width of the smallest seed of the current collection batch, said long portion (121222) being larger than the length of the largest seed of the current collection batch, said second high portion (121223) being no more than half the height of the seed of the current collection batch.
4. The daughter disc of claim 3, wherein said circular groove (12121) comprises a first high portion (121211), said circular groove (12121) having a diameter of 11.0mm, said first high portion (121211) having a height of 5.0mm, said wide portion (121221) of said rectangular groove (12122) having a length dimension of 6.3mm, said long portion (121222) having a length dimension of 9.0mm, said second high portion (121223) having a height dimension of 3.5 mm.
5. The seed real disk capable of collecting soybean hilum phenotype image data according to claim 1, characterized in that said dial (12) comprises a checking area (122) and an information placing area (123), a color chart is placed in said checking area (121) for performing color check on the final image of the seed placed on said seed placing area (121), and said information placing area (123) is used for placing the seed coding information and other related texts of the current shooting batch.
6. The daughter disc for collecting soybean umbilicus phenotype image data according to claim 1, wherein the transverse side and the longitudinal side of the seed placement area (121) are provided with row and column marks (1211).
7. The sub-real disk capable of acquiring soybean hilum phenotype image data according to claim 1, wherein the base (11) comprises a hand-held hole (113), and the hand-held hole (113) is concavely arranged on four borders of the base (11).
8. The daughter board capable of collecting soybean hilum phenotype image data according to claim 1, wherein said base (11) comprises a reinforcing plate (111), said reinforcing plate (111) is disposed at the bottom of said base (11) for reinforcing the strength of said base (11).
9. A method for acquiring phenotype data by using the daughter disc capable of acquiring soybean hilum phenotype image data of any one of claims 1 to 8, comprising the following steps:
step1, placing a color comparison card in a verification area (122) of the sub-real disc (1), and placing a two-dimensional code in an information placing area (123);
step2, placing seeds of the seeds to be tested on a placing groove (1212) of the seed real tray (1), and poking the seeds by using tweezers to enable the umbilicus of the seeds to be placed upwards;
step3, placing the sub real disc (1) at a specified position, placing a camera device and a light source above the sub real disc (1), and taking a picture of the sub real disc (1) by adopting the camera device and storing the taken picture;
step4, taking a batch of seeds of the seeds to be tested, if the number of the soybean variety is not changed, turning to Step2 to continue repeating the steps, and if the number of the soybean variety is changed, turning to Step1 to continue repeating the steps;
step5, transmitting the image files of the plurality of shot seeds to a designated cloud server;
step6, calling an image processing program by the cloud server to process the image file in parallel, extracting the phenotype characteristic data of the seeds, the color comparison card information of the verification area (122) and the two-dimensional code type number of the information placement area (123), and storing the phenotype characteristic data, the color comparison card information and the two-dimensional code type number in a phenotype database;
and Step7, if the seeds of the seeds to be tested still exist, turning to Step1 to continue repeating the steps, and if not, finishing the acquisition of the soybean hilum phenotype image data.
10. Method for the acquisition of phenotypic data according to claim 9, comprising, after the step of Step4 and before the step of Step5, the following steps:
and step41, placing a pure white color comparison card in front of the camera equipment, completely covering the seeds on the seed solid disc (1), photographing the color comparison card, and then taking away the color comparison card.
CN201911310763.5A 2019-12-18 2019-12-18 Seed disk capable of collecting soybean hilum phenotype image data and collection method thereof Pending CN110865080A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112793991A (en) * 2020-12-24 2021-05-14 杭州电子科技大学 Soybean seed test information acquisition method
CN117314716A (en) * 2023-09-06 2023-12-29 深圳市农业科技促进中心 Seed characteristic acquisition equipment and seed characteristic acquisition method

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112793991A (en) * 2020-12-24 2021-05-14 杭州电子科技大学 Soybean seed test information acquisition method
CN112793991B (en) * 2020-12-24 2022-04-15 杭州电子科技大学 Soybean seed test information acquisition method
CN117314716A (en) * 2023-09-06 2023-12-29 深圳市农业科技促进中心 Seed characteristic acquisition equipment and seed characteristic acquisition method

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