CN111334417A - Pre-packaging magnetic bead method nucleic acid extraction device - Google Patents
Pre-packaging magnetic bead method nucleic acid extraction device Download PDFInfo
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- CN111334417A CN111334417A CN202010292138.9A CN202010292138A CN111334417A CN 111334417 A CN111334417 A CN 111334417A CN 202010292138 A CN202010292138 A CN 202010292138A CN 111334417 A CN111334417 A CN 111334417A
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- reagent
- rod sleeve
- magnetic rod
- magnetic
- reagent tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
Abstract
The invention relates to a nucleic acid extraction device by a pre-distribution magnetic bead method, which comprises a reagent plate and a magnetic rod sleeve; the reagent plate is formed by fixing a plurality of reagent strips into a whole, and an easily broken and split breakpoint is arranged between every two adjacent reagent strips; the reagent strip is provided with at least one cracking reagent tube, at least one cleaning reagent tube, an elution reagent tube, a magnetic bead reagent tube and a magnetic rod sleeve reagent tube, wherein the diameter of the outer circle of the magnetic rod sleeve is smaller than the diameter of the inner circle of the magnetic rod sleeve reagent tube, and the magnetic rod sleeve is pre-embedded in the magnetic rod sleeve reagent tube. In the invention, a single reagent strip has small volume, small space occupation and convenient carrying and use; the used reagent is a pre-assembled detachable reagent plate, and can be flexibly detached for use according to the flux requirement, so that the problem of reagent cost waste is solved; the magnetic rod sleeve is packaged in the pre-installed reagent plate, and the machine is automatically installed and disassembled without manual operation, so that the use is convenient and fast.
Description
Technical Field
The invention relates to the technical field of nucleic acid extraction devices, in particular to a nucleic acid extraction device adopting a pre-distribution magnetic bead method.
Background
Compared with immunological detection, cytology detection and the like, the nucleic acid detection technology has higher sensitivity, specificity and rapidness. The key point of the nucleic acid detection technology is to separate the nucleic acid of a biological sample, and an effective and accurate nucleic acid extraction technology is the premise of subsequent detection.
At present, the traditional manual method is mainly adopted for extracting nucleic acid in China, and the method is low in efficiency, high in cost, and poor in stability and repeatability. Some laboratories are equipped with automated nucleic acid extraction devices that can achieve high throughput extraction, but the current fully automated nucleic acid extraction devices have the following drawbacks and deficiencies: (1) the volume is large, and the movement cannot be convenient; (2) flux cannot be flexibly adjusted, and when a small amount of sample is extracted, 16-well or 96-well pre-packaged plate reagents are wasted; (3) the magnetic rod sleeve needs to be manually installed and detached, the operation is not simple and convenient enough, and human errors are easy to occur; (4) the auxiliary drying function is not provided, the waiting time is long, and alcohol residue is also generated; (5) the magnetic rod sleeve is unreasonable in design, and the gathered magnetic beads can be scattered only by fast flapping speed, so that aerosol pollution is easily caused.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provide the nucleic acid extraction device by the pre-split magnetic bead method, which can be flexibly used in a split manner and avoid reagent waste.
According to the technical scheme provided by the invention, the pre-subpackaging magnetic bead method nucleic acid extraction device comprises a reagent plate and a magnetic rod sleeve; the reagent plate is formed by fixing a plurality of reagent strips into a whole, and an easily broken and split breakpoint is arranged between every two adjacent reagent strips; the reagent strip is provided with at least one cracking reagent tube, at least one cleaning reagent tube, an elution reagent tube, a magnetic bead reagent tube and a magnetic rod sleeve reagent tube, wherein the diameter of the outer circle of the magnetic rod sleeve is smaller than the diameter of the inner circle of the magnetic rod sleeve reagent tube, and the magnetic rod sleeve is pre-embedded in the magnetic rod sleeve reagent tube.
Preferably, the reagent strip is further provided with a dry reagent tube, and the lysis reagent tube, the cleaning reagent tube, the magnetic bead reagent tube and the magnetic rod sleeve reagent tube can be arranged in a straight line or a circle.
Preferably, the magnetic rod sleeve is provided with an upper section, a middle section and a lower section, and the excircle diameter of the lower section of the magnetic rod sleeve is larger than that of the middle section of the magnetic rod sleeve.
Preferably, a gap of 0.1-3mm is formed between the outer circle of the lower section of the magnetic rod sleeve and the inner circle of the magnetic rod sleeve reagent tube.
Preferably, the heights of the lysis reagent tube, the cleaning reagent tube and the magnetic bead reagent tube are smaller than the height of the magnetic rod sleeve.
Preferably, the number of the cleaning reagent tubes is 3.
Preferably, the reagent plate is formed by fixing 1-12 reagent strips into a whole.
Preferably, the reagent strip or the reagent plate can be sealed by using an aluminum plastic film or an adhesive film.
The invention has the following advantages:
③ the magnetic bar sleeve is packaged in the pre-installed reagent plate, the machine is automatically installed and disassembled, manual operation is not needed, and the use is convenient;
④ drying tube is designed to accelerate drying speed and reduce alcohol residue;
⑤ the unique magnetic bar sleeve structure can easily break up the aggregated magnetic beads, increase nucleic acid yield, reduce beating speed, and prevent aerosol pollution and inter-pore pollution.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention.
FIG. 2 is a schematic structural diagram of the reagent strip of the present invention when the magnetic rod sleeve is not pulled out.
FIG. 3 is a schematic view of the reagent strip of the present invention with the magnetic sleeve partially removed.
Fig. 4 is a schematic structural view of the magnet sleeve of the present invention.
Detailed Description
The present invention will be further described with reference to the following specific examples.
The invention discloses a nucleic acid extraction device by a pre-subpackaging magnetic bead method, which comprises a reagent plate 2 and a magnetic rod sleeve 4; the reagent plate 2 is formed by fixing a plurality of reagent strips 1 into a whole, and an easily broken and broken splitting breakpoint 3 is arranged between every two adjacent reagent strips 1; the reagent strip 1 is provided with at least one cracking reagent tube 11, at least one cleaning reagent tube 12, an elution reagent tube 14, a magnetic bead reagent tube 15 and a magnetic rod sleeve reagent tube 16, the diameter of the outer circle of the magnetic rod sleeve 4 is smaller than that of the inner circle of the magnetic rod sleeve reagent tube 16, and the magnetic rod sleeve 4 is pre-embedded in the magnetic rod sleeve reagent tube 16.
The reagent strip 1 is also provided with a dry reagent tube 13, and the cracking reagent tube 11, the cleaning reagent tube 12, the magnetic bead reagent tube 15 and the magnetic rod sleeve reagent tube 16 can be arranged in a straight line or a circle.
The magnetic rod sleeve 4 is provided with an upper section, a middle section and a lower section, and the excircle diameter of the lower section of the magnetic rod sleeve 4 is larger than that of the middle section of the magnetic rod sleeve 4. And a gap of 0.1-3mm is formed between the excircle of the lower section of the magnetic rod sleeve 4 and the inner circle of the magnetic rod sleeve reagent tube 16.
The heights of the lysis reagent tube 11, the cleaning reagent tube 12 and the magnetic bead reagent tube 15 are less than the height of the magnetic rod sleeve 4.
The number of the cleaning reagent tubes 12 is 3.
The reagent plate 2 is formed by fixing 1-12 reagent strips 1 into a whole.
The reagent strip 1 or the reagent plate 2 can be sealed by using an aluminum plastic film or an adhesive film.
In the invention, the pre-installed reagent plate 2 consists of 12 single-row reagent strips 1, the design can be split, and each reagent strip 1 can be used independently. Specifically, reagent strip 1 uses hydrophobic material, has between the reagent strip 1 and easily breaks disconnected split breakpoint 3, can break disconnected split easily off with the fingers and thumb, takes the reagent strip according to the sample quantity is nimble, can avoid traditional nucleic acid automatic extraction equipment to cause 16 holes or 96 holes pre-installation board reagent extravagant completely.
The magnetic rod sleeve 4 is pre-embedded in the reagent strip 1 and is designed with a separate magnetic rod sleeve reagent tube 16. Specifically, the tail of the magnetic rod sleeve is designed to be conical, and a conical plug is inserted into the tail of the magnetic rod sleeve by an instrument and is closely matched with the magnetic rod sleeve by means of elastic deformation and friction force, so that the magnetic rod sleeve 4 is automatically grabbed. After the extraction work is finished, the magnetic rod sleeve 4 returns to the magnetic rod sleeve reagent tube 16 and is pressed down by touching the bottom of the magnetic rod, so that the magnetic rod sleeve 4 is automatically detached. The whole extraction process is automatically completed. The lower section of the magnetic rod sleeve 4 adopts a thickening design, the gap between the magnetic rod sleeve and the tube wall of the reagent tube 16 of the magnetic rod sleeve is preferably set to be 0.2mm, the flowing gap of the solution is reduced, the gathered magnetic beads are easy to break up, the nucleic acid yield is improved, the beating speed can be reduced, and aerosol pollution and inter-hole pollution are prevented.
In the invention, the three cleaning reagent tubes 12 can ensure that the magnetic beads are cleaned more fully, the purity of the obtained nucleic acid is higher, and the detection inhibitor is less. The independent drying reagent tube 13 can be dried by machine heating and ventilation assistance of a fan, the drying speed is higher, and the residual alcohol is less.
Specifically, the magnetic rod sleeve 4 and the reagent strip 1 are made of PP materials, so that the magnetic absorption, demagnetization and mechanical stirring efficiencies are high, and the uniformity is higher. Each single reagent strip 1 is provided with 8 reagent tubes, including a cracking reagent tube 11, a magnetic bead reagent tube 15, 3 cleaning reagent tubes 12, a drying reagent tube 13, an elution reagent tube 14 and a magnetic rod sleeve reagent tube 16. The handle 18 at the rear end of the reagent strip 1 is in accordance with human engineering and is convenient to hold.
The corresponding extraction process comprises the following steps: the instrument firstly drives the worktable to automatically install the magnetic rod sleeve 4, and then the magnetic beads in the magnetic bead reagent tube 15 are adsorbed at the front end of the magnetic rod sleeve 4 and transferred to the cracking reagent tube 11. After the cracking is finished, the magnetic bar adsorbs magnetic beads to be washed for the first time, the second time and the third time in the three washing reagent tubes 12, then the magnetic beads are transferred to the drying reagent tube 13, the heating module starts to heat, the rapid drying is assisted, and the nucleic acid is eluted in the elution reagent tube 14 after the drying. After the extraction work is finished, the magnetic rod sleeve 4 returns to the magnetic rod sleeve reagent tube 16 and is pressed down by touching the bottom of the magnetic rod, so that the magnetic rod sleeve 4 is automatically detached. The whole extraction process does not need manual loading and unloading, and placement errors are avoided.
Compared with the traditional 16-hole or 96-hole reagent plate which can only be washed by magnetic beads for 2 times, the three washing reagent tubes 12 in the invention can ensure that the magnetic beads are washed more fully, the purity of the obtained nucleic acid is higher, and the detection inhibitor is less. The independent drying reagent tube 13 can be heated by a machine-controlled heating module, the drying speed is higher, and the residual alcohol is less. The nucleic acid extraction device adopting the pre-distribution magnetic bead method provided by the invention solves the problems of more manual operations, reagent waste, long extraction time, high cross contamination probability and the like of the traditional automatic nucleic acid extraction equipment at one time, and has the advantages of small volume, random movement and capability of adapting to various extraction environments.
In the invention, the magnetic rod sleeve 4 is pre-embedded in the reagent strip 1, when in use, the magnetic rod sleeve 4 is automatically grabbed by an instrument without manual placement, so that placement errors are avoided, and the magnetic rod sleeve 4 can be automatically detached in the magnetic rod sleeve reagent tube 16 by the instrument after use.
The invention adopts a detachable pre-assembled reagent plate 2, and the reagent plate 2 consists of 12 reagent strips 1 and can be freely detached and placed among 1 to 12 reagent strips.
In the invention, the lower section of the magnetic rod sleeve 4 is thickened, and a gap of 0.1-3mm is formed between the lower section of the magnetic rod sleeve and the inner wall of the magnetic rod sleeve reagent tube 16, so that the gathered magnetic beads are easy to break up, the nucleic acid yield is improved, the beating speed is reduced, and aerosol pollution and inter-hole pollution are prevented.
In the present invention, a drying reagent tube 13 may be provided to accelerate the drying speed and reduce the alcohol residue.
Claims (8)
1. A nucleic acid extraction device adopting a pre-subpackaging magnetic bead method comprises a reagent plate (2) and a magnetic rod sleeve (4); the reagent plate (2) is formed by fixing a plurality of reagent strips (1) into a whole, and an easily broken and broken splitting breakpoint (3) is arranged between every two adjacent reagent strips (1); the method is characterized in that: the reagent strip (1) is provided with at least one cracking reagent tube (11), at least one cleaning reagent tube (12), an elution reagent tube (14), a magnetic bead reagent tube (15) and a magnetic rod sleeve reagent tube (16), the diameter of the outer circle of the magnetic rod sleeve (4) is smaller than the diameter of the inner circle of the magnetic rod sleeve reagent tube (16), and the magnetic rod sleeve (4) is pre-embedded in the magnetic rod sleeve reagent tube (16).
2. The apparatus for nucleic acid isolation by pre-dispensing magnetic beads according to claim 1, wherein: the reagent strip (1) is also provided with a dry reagent tube (13), and the cracking reagent tube (11), the cleaning reagent tube (12), the magnetic bead reagent tube (15) and the magnetic rod sleeve reagent tube (16) can be arranged in a straight line or a circular shape.
3. The apparatus for nucleic acid isolation by pre-dispensing magnetic beads according to claim 1, wherein: the magnetic rod sleeve (4) is provided with an upper section, a middle section and a lower section, and the excircle diameter of the lower section of the magnetic rod sleeve (4) is larger than that of the middle section of the magnetic rod sleeve (4).
4. The apparatus for nucleic acid isolation by pre-dispensing magnetic beads according to claim 3, wherein: and a gap of 0.1-3mm is formed between the excircle of the lower section of the magnetic rod sleeve (4) and the inner circle of the magnetic rod sleeve reagent tube (16).
5. The apparatus for nucleic acid isolation by pre-dispensing magnetic beads according to claim 1, wherein: the heights of the lysis reagent tube (11), the cleaning reagent tube (12) and the magnetic bead reagent tube (15) are less than the height of the magnetic rod sleeve (4).
6. The apparatus for nucleic acid isolation by pre-dispensing magnetic beads according to claim 1, wherein: the number of the cleaning reagent tubes (12) is 3.
7. The apparatus for nucleic acid isolation by pre-dispensing magnetic beads according to claim 1, wherein: the reagent plate (2) is formed by fixing 1-12 reagent strips (1) into a whole.
8. The apparatus for nucleic acid isolation by pre-dispensing magnetic beads according to claim 1, wherein: the reagent strip (1) or the reagent plate (2) can be sealed by using an aluminum plastic film or an adhesive film.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010292138.9A CN111334417A (en) | 2020-04-14 | 2020-04-14 | Pre-packaging magnetic bead method nucleic acid extraction device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010292138.9A CN111334417A (en) | 2020-04-14 | 2020-04-14 | Pre-packaging magnetic bead method nucleic acid extraction device |
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| Publication Number | Publication Date |
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| CN111334417A true CN111334417A (en) | 2020-06-26 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202010292138.9A Pending CN111334417A (en) | 2020-04-14 | 2020-04-14 | Pre-packaging magnetic bead method nucleic acid extraction device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113201529A (en) * | 2021-04-30 | 2021-08-03 | 杭州米欧仪器有限公司 | Nucleic acid extraction method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140206088A1 (en) * | 2011-09-30 | 2014-07-24 | Becton, Dickinson And Company | Unitized reagent strip |
| CN205874382U (en) * | 2016-08-17 | 2017-01-11 | 杭州奥盛仪器有限公司 | Change nucleic acid extraction equipment of mixing mode |
| CN206680511U (en) * | 2017-03-03 | 2017-11-28 | 安徽为臻生物工程技术有限公司 | A kind of electromagnetic type nucleic acid-extracting apparatus |
| CN110819627A (en) * | 2019-11-29 | 2020-02-21 | 杭州博日科技有限公司 | Multi-column single tube consumable and use method thereof in nucleic acid extraction and purification by magnetic rod method |
-
2020
- 2020-04-14 CN CN202010292138.9A patent/CN111334417A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140206088A1 (en) * | 2011-09-30 | 2014-07-24 | Becton, Dickinson And Company | Unitized reagent strip |
| CN205874382U (en) * | 2016-08-17 | 2017-01-11 | 杭州奥盛仪器有限公司 | Change nucleic acid extraction equipment of mixing mode |
| CN206680511U (en) * | 2017-03-03 | 2017-11-28 | 安徽为臻生物工程技术有限公司 | A kind of electromagnetic type nucleic acid-extracting apparatus |
| CN110819627A (en) * | 2019-11-29 | 2020-02-21 | 杭州博日科技有限公司 | Multi-column single tube consumable and use method thereof in nucleic acid extraction and purification by magnetic rod method |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113201529A (en) * | 2021-04-30 | 2021-08-03 | 杭州米欧仪器有限公司 | Nucleic acid extraction method |
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