CN219342169U

CN219342169U - Deep hole plate based on magnetic bead method nucleic acid extraction

Info

Publication number: CN219342169U
Application number: CN202320251175.4U
Authority: CN
Inventors: 韩凯; 许墨耘
Original assignee: Yali Jiangsu Biotechnology Co ltd
Current assignee: Yali Jiangsu Biotechnology Co ltd
Priority date: 2023-02-20
Filing date: 2023-02-20
Publication date: 2023-07-14
Anticipated expiration: 2033-02-20

Abstract

The utility model discloses a deep hole plate based on magnetic bead method nucleic acid extraction, which comprises a box body and a plurality of deep holes fixedly arranged in the box body, wherein the plurality of deep holes are arranged in a rectangular shape, the cross sections of the deep holes are square, the plurality of deep holes comprise n large deep holes and 96-4n small deep holes, and the side length of the cross section of the large deep hole is twice the side length of the cross section of the small deep hole. The utility model can realize the change of the volume of the solution from large to small through the operation of the existing nucleic acid extractor, and the utility model can enhance the experimental capability of large-volume nucleic acid extraction of the existing nucleic acid extractor with lower cost.

Description

Deep hole plate based on magnetic bead method nucleic acid extraction

Technical Field

The utility model relates to a medical experiment device, in particular to a deep pore plate based on magnetic bead method nucleic acid extraction.

Background

Magnetic beads are a special class of nano-micrometer materials whose size is typically between a few tenths of a micrometer to a few micrometers, just a tenth to a tenth of the diameter of a hair strand, and individual magnetic beads are not visible to the naked eye. They have superparamagnetism, and can move to one side rapidly in a magnetic field, gather together, and can be restored to a dispersed state rapidly after the magnetic field is removed.

Finally, the surface of the magnetic beads used for nucleic acid extraction has specific properties, and can adsorb viral nucleic acid on the surface under certain conditions, and release viral nucleic acid from the surface under other conditions for subsequent detection steps. Binding of nucleic acids to magnetic beads relies mainly on electrostatic interactions, hydrophobic interactions and hydrogen bonding. The cells or tissues are subjected to lysis and the DNA/RNA therein is released. The superparamagnetism silica nano magnetic beads subjected to surface modification are subjected to specific binding with nucleic acid to form a nucleic acid-magnetic bead complex. Then under the action of the external magnetic field, the compound is separated. Finally, washing off the nonspecifically adsorbed magazines by using an eluent, desalting and purifying to obtain the nucleic acid substance to be extracted.

The magnetic bead method nucleic acid extraction can realize automation and large-batch operation. At present, 96-hole automatic nucleic acid extractors exist on the market, 96 samples can be processed by using one sample extraction time, and the automatic nucleic acid extractor meets the high-flux operation requirement of biology, so that rapid and timely coping can be performed when infectious diseases burst. The operation is simple, the time is short, the whole extraction process is only four steps, and most of the extraction process can be completed within 36-40 minutes. The method is safe and nontoxic, does not use toxic reagents such as benzene, chloroform and the like in the traditional method, reduces the injury to experimental operators to the minimum, and completely accords with the modern environment-friendly concept. The specificity combination of the magnetic beads and the nucleic acid ensures that the extracted nucleic acid has high purity and high concentration.

In the prior art, a fully automatic nucleic acid extractor is generally used for extracting nucleic acid corresponding to a small-volume sample or a large-volume sample according to the preset use requirement, and the nucleic acid extractor capable of simultaneously processing two different requirements is lacking. The main problem is that the volumes of reagents used in all steps in the process of extracting the nucleic acid from the small-volume sample are basically consistent, and all required reagents can be packaged by using holes with uniform volume size, so that a standard universal 96 deep hole plate can be used, and the nucleic acid extractor for the small-volume sample has very high universality; for the treatment of large-volume samples, the volumes required by the different reagents are greatly different, and the volumes of the reagents need to be gradually reduced in the nucleic acid extraction process so as to finally obtain nucleic acid eluent with proper concentration, so that hole sites with different volumes need to be respectively designed for the reagents in different steps, the nucleic acid extraction process of the large-volume samples is different, and the nucleic acid extractor used has no universality.

Disclosure of Invention

In order to solve the technical problems, the utility model aims to provide a deep pore plate with large volume and small volume universality for the existing nucleic acid extractor based on the magnetic bead method for extracting nucleic acid.

In order to achieve the above purpose, the technical scheme of the utility model is that the deep hole plate based on the magnetic bead method nucleic acid extraction comprises a box body and a plurality of deep holes fixedly arranged in the box body, wherein the plurality of deep holes are arranged in a rectangular shape, the cross sections of the deep holes are square, the plurality of deep holes comprise n large deep holes and 96-4n small deep holes, and the side length of the cross section of the large deep holes is twice the side length of the cross section of the small deep holes.

In the above technical scheme, when the number of the large deep holes is 8, the number of the small deep holes is 64.

According to the preferable technical scheme, the large deep hole comprises a first step hole and a second step hole, and the central axis of the first step hole coincides with the central axis of the second step hole.

According to a further technical scheme, the depth of the first step hole is the same as that of the second step hole, and the cross sections of the first step hole and the second step hole are square.

According to a further technical scheme, the cross section side length of the first step hole is twice that of the second step hole.

According to the preferable technical scheme, the depth of the large deep hole is the same as that of the small deep hole.

The volume of the small deep hole is 1ml, and the volume of the large deep hole is 2.5ml.

According to the preferred technical scheme, the cross section of the box body is rectangular, two scales are arranged on the upper surface of the box body, and the two scales are mutually perpendicular.

In the technical scheme, the two scales are a transverse scale and a vertical scale, the transverse scale is provided with a digital label, and the vertical scale is provided with an alphabetical scale.

The utility model has the advantages that:

1. the external dimension of the utility model is the same as that of a universal 96 deep-hole plate, and the utility model can be directly matched with the existing automatic nucleic acid extractor;

2. the utility model can realize the change of the volume of the solution from large to small through the operation of the existing nucleic acid extractor, and the utility model can enhance the experimental capability of large-volume nucleic acid extraction of the existing nucleic acid extractor with lower cost.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the drawings that are needed in the description of the embodiments will be briefly described below, in which the drawings are only some embodiments of the present utility model, and other drawings can be obtained according to these drawings without inventive effort for a person skilled in the art.

The utility model is further described below with reference to the accompanying drawings and examples:

FIG. 1 is a schematic diagram of the structure of the present utility model;

FIG. 2 is a top view of the present utility model;

fig. 3 is a semi-sectional view of the present utility model.

Wherein: 1. a case body; 2. a large deep hole; 3. a small deep hole; 4. a first stepped hole; 5. a second stepped hole; 6. a transverse scale; 7. vertical scale.

Detailed Description

Examples: as shown in figures 1-3, the deep hole plate based on magnetic bead method nucleic acid extraction comprises a box body 1 and a plurality of deep holes fixedly arranged in the box body 1, wherein the plurality of deep holes are arranged in a rectangular shape, the cross section of each deep hole is square, the plurality of deep holes comprise eight large deep holes 2 and sixty four small deep holes 3, and the side length of the cross section of each large deep hole 2 is twice the side length of the cross section of each small deep hole 3.

The length of the box body 1 is 127mm, the width is 85mm, the height is 44mm, the depth of the small deep hole 3 is 41.5mm, the width is 12mm, and the depth of the large deep hole 2 is 41.5mm, and the width is 24mm.

The volume of the small deep hole 3 is 1ml, and the volume of the large deep hole 2 is 2.5ml.

The deep hole 2 comprises a first step hole 4 and a second step hole 5, and the central axis of the first step hole 4 coincides with the central axis of the second step hole 5.

The first stepped hole 4 has a width of 24mm and a depth of 20mm, and the second stepped hole 5 has a width of 12mm and a depth of 21.5mm.

The depth of the first step hole 4 is the same as that of the second step hole 5, and the cross sections of the first step hole 4 and the second step hole 5 are square.

The depth of the large deep hole 2 is the same as the depth of the small deep hole 3.

The cross section of the box body 1 is rectangular, two scales are arranged on the upper surface of the box body 1, and the two scales are mutually perpendicular.

The two scales are a transverse scale 6 and a vertical scale 7, the transverse scale 6 is provided with digital labels 1-12, and the vertical scale 7 is provided with an alphabetical scale A-H.

The application method of the embodiment comprises the following steps:

the utility model is used together with nucleic acid extraction equipment, a sample and a sample treatment reagent are contained in a large deep hole 2, and a rinsing reagent and an eluent are contained in a small deep hole 3. Each large deep hole 2 is matched with six small deep holes 3 as a group for nucleic acid extraction. Eight extraction reactions can be handled per cartridge 1.

After a sample is processed in a large deep hole 2, magnetic beads are adsorbed on the sample, the magnetic beads are transferred to rinsing holes of a small deep hole 3 through corresponding magnetic sleeve magnetic rods to be rinsed, the rinsing times are set according to different sample types, at most five times of rinsing can be performed, each group of extraction reaction holes (large deep holes 2), the last small deep hole 3 is used as an elution hole, the small deep hole 3 responsible for eluting holds an elution solution, and the magnetic beads enter the elution hole (the last small deep hole 3) to perform nucleic acid elution after the rinsing is completed. And finally, completing the nucleic acid extraction process.

Claims

1. The utility model provides a deep hole board based on magnetic bead method nucleic acid extraction, includes the box body, fixedly sets up a plurality of deep holes in the box body, its characterized in that: the deep holes are arranged in a rectangular mode, the cross sections of the deep holes are square, the deep holes comprise n large deep holes and 96-4n small deep holes, and the side length of the cross section of each large deep hole is twice that of the cross section of each small deep hole.

2. The deep well plate based on magnetic bead method nucleic acid extraction according to claim 1, wherein: the large deep hole comprises a first step hole and a second step hole, and the central axis of the first step hole coincides with the central axis of the second step hole.

3. The deep well plate based on magnetic bead method nucleic acid extraction according to claim 2, wherein: the depth of the first step hole is the same as that of the second step hole, and the cross sections of the first step hole and the second step hole are square.

4. A deep well plate based on magnetic bead method nucleic acid extraction according to claim 3, characterized in that: the cross-sectional side length of the first step hole is twice that of the second step hole.

5. The deep well plate based on magnetic bead method nucleic acid extraction according to claim 1, wherein: the depth of the large deep hole is the same as that of the small deep hole.

6. The deep well plate based on magnetic bead method nucleic acid extraction according to claim 1, wherein: the volume of the small deep hole is 1ml, and the volume of the large deep hole is 2.5ml.

7. The deep well plate based on magnetic bead method nucleic acid extraction according to claim 1, wherein: the cross section of the box body is rectangular, two scales are arranged on the upper surface of the box body, and the two scales are mutually perpendicular.

8. The deep well plate based on magnetic bead method nucleic acid extraction according to claim 1, wherein: the length of the box body is 127mm, the width of the box body is 85mm, the height of the box body is 44mm, the depth of the small deep hole is 41.5mm, the width of the small deep hole is 12mm, and the depth of the large deep hole is 41.5mm, and the width of the large deep hole is 24mm.

9. A deep well plate based on magnetic bead method nucleic acid extraction according to claim 3, characterized in that: the width of the first step hole is 24mm, the depth is 20mm, and the width of the second step hole is 12mm, and the depth is 21.5mm.