CN215560195U - Plain type nucleic acid extraction element - Google Patents
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- CN215560195U CN215560195U CN202121131312.8U CN202121131312U CN215560195U CN 215560195 U CN215560195 U CN 215560195U CN 202121131312 U CN202121131312 U CN 202121131312U CN 215560195 U CN215560195 U CN 215560195U
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Abstract
The utility model discloses a simple nucleic acid extraction device. The device comprises a magnetic bar rack, a magnetic bar sleeve rack and a deep hole plate; the magnetic rod rack comprises a first magnetic rod rack; the first magnetic rod rack comprises a first magnetic rod seat and a plurality of magnetic rods fixed on the first magnetic rod seat; the magnetic bar sleeve frame comprises a first magnetic bar sleeve frame; the first magnetic bar sleeve frame comprises a first magnetic bar sleeve seat and a plurality of magnetic bar sleeves fixed on the first magnetic bar sleeve seat; the magnetic rod sleeve fixed on the first magnetic rod sleeve seat is matched with the magnetic rod fixed on the first magnetic rod seat; the deep well plate includes a first extraction zone; the first extraction area comprises a first lysis solution area, a first washing solution drying area and a first nucleic acid amplification reagent area in sequence, and the deep hole of each area is matched with a magnetic rod sleeve fixed on a first magnetic rod sleeve seat. The magnetic rod type nucleic acid extraction device can overcome the defects of small flux, complex operation, long time consumption and the like of a liquid-transfer type magnetic bead extraction method, and reduces the pressure of manually extracting nucleic acid.
Description
Technical Field
The utility model relates to a nucleic acid extraction device, in particular to a simple nucleic acid extraction device, and belongs to the field of biological medicines.
Background
In the period of infectious disease prevention and control, timely finding out an infection source and infected people are important, the use of a high-efficiency and sensitive detection method is the most important link for preventing and cutting off epidemic spread, and an antigen-antibody detection method is used in the early stage. The nucleic acid detection well overcomes the defect that the virus cannot be detected because the virus carrying capacity is far lower than the sensitivity of an antigen-antibody detection method, and is now the main means of virus detection. As an important link of molecular diagnosis, the quality of purified nucleic acid affects the positive detection rate of downstream detection.
The main process of nucleic acid detection comprises: collecting a sample, extracting nucleic acid, amplifying the nucleic acid and analyzing and detecting the nucleic acid. Wherein the nucleic acid extraction step mainly comprises: inactivating and cracking the virus, specifically adsorbing the nucleic acid on the carrier, eluting the purified nucleic acid, and mixing the nucleic acid with an amplification reaction reagent for reaction. The lysis solution generally contains a strong protein denaturant, which can promote the rupture of cells to release nucleic acid from the cells, and can inhibit the activity of nuclease to reduce the degradation of the nucleic acid. The solid phase carrier adsorption method of the prior research methods includes: the method comprises a rotary centrifugal column extraction method, a glass bead adsorption method, a silicon dioxide matrix method, an anion exchange method and a nano magnetic bead extraction method, wherein the magnetic beads have the characteristics of easy modification, high specific surface area, high nucleic acid adsorption amount and the like compared with other solid phase carriers. The carrier has strong affinity and adsorption force to nucleic acid, specificity is combined with nucleic acid, other substances in cells are still dissociated in a liquid phase, the effect of enriching and purifying nucleic acid is achieved by removing the liquid phase, then the solid phase carrier is washed, impurities on the surface of the solid phase carrier are removed, the washing liquid mostly adopts ethanol diluent, and the ethanol has a great inhibition effect on a nucleic acid amplification system in subsequent molecular diagnosis and needs to be kept stand to volatilize during nucleic acid extraction. Directly adding the solid phase carrier into a nucleic acid amplification system can inhibit the reaction to a certain extent, so that the nucleic acid is eluted from the solid phase carrier by using an eluent, and then the eluent is added into a nucleic acid amplification solution and uniformly mixed for carrying out amplification reaction and detection. The current gold standard for nucleic acid detection is qPCR, i.e. fluorescent quantitative PCR, which utilizes fluorescent probes or nucleic acid dyes to bind to the target DNA, releasing an optical signal, which is collected and analyzed by an optical detection element. When target nucleic acid exists, the primer is specifically combined with the target to trigger reaction, the nucleic acid is amplified in a number-step mode, signals are amplified in a number-step mode, and the sensitivity of nucleic acid detection is greatly improved.
A great number of mature kits on the market adopt a centrifugal column method, which has the main advantages of low price and convenient operation, but the disadvantages of the method are gradually exposed along with the increase of the sample amount, the steps are multiple, the time consumption is long, the requirement for sample extraction is large, the loss in the extraction process is high, and the bottleneck of the centrifugal column method is caused by the unfriendliness of rare samples. When the centrifugal column method is used for extracting DNA, a high-speed centrifuge is frequently used for repeated centrifugation, and the application scene of the method is limited. Modern molecular biology mainly has three changing trends, namely high-throughput quantification, high-sensitivity quantification and automation. Clearly, the development of the spin column method is not able to keep up with modern biological developments.
Therefore, the generation of the 20 th century and the nineties' magnetic bead method has become the mainstream of the development of the times, nucleic acid is specifically combined with the magnetic bead under specific conditions by modifying a group on the surface of the magnetic bead, and the magnetic bead is directionally moved and enriched under the action of an external magnetic field by utilizing the superparamagnetism of the magnetic bead, so that the separation and the purification of the nucleic acid are realized. When the sample volume of nucleic acid processing does not reach a certain amount, the automatic equipment is used for processing, reagent waste and cost increase are caused, and the automatic nucleic acid extractor on the market is very expensive, the occupied area of the instrument is large, and consumable materials are not cheap, so that the manual extraction of nucleic acid has the application scene and the existing need.
At present, most of nucleic acid extraction is carried out manually by adopting a pipetting type magnetic bead extraction method, the pipetting gun and a magnetic rack are utilized to realize the pipetting of solution and the transfer of magnetic beads, but the pipetting gun is frequently used to cause low flux of manually extracting nucleic acid and low efficiency, for example, for personnel who are unfamiliar with operation, the phenomenon that washing liquid is not removed by the last centrifugal tubes when parallel samples are processed can easily occur, the ethanol which is processed the samples at first is dried, and the degradation of nucleic acid can be caused when the magnetic beads are exposed in the air for a long time. The increase in the amount of nucleic acid to be processed also causes human errors in nucleic acid extraction.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a simple nucleic acid extraction device which can overcome the defects of small flux, complex operation, long time consumption and the like of a liquid-transferring magnetic bead extraction method and can reduce the pressure of manually extracting nucleic acid.
The utility model provides a nucleic acid extraction device, which comprises a magnetic rod rack, a magnetic rod sleeve rack and a deep hole plate, wherein the magnetic rod sleeve rack is arranged on the magnetic rod rack;
the magnetic rod rack comprises a first magnetic rod rack; the first magnetic rod rack comprises a first magnetic rod seat and a plurality of magnetic rods fixed on the first magnetic rod seat;
the magnetic bar sleeve frame comprises a first magnetic bar sleeve frame; the first magnetic bar sleeve frame comprises a first magnetic bar sleeve seat and a plurality of magnetic bar sleeves fixed on the first magnetic bar sleeve seat; the magnetic rod sleeve fixed on the first magnetic rod sleeve seat is matched with the magnetic rod fixed on the first magnetic rod seat;
the deep well plate comprises a first extraction zone; the first extraction area sequentially comprises a first lysate containing area, a first washing solution drying area and a first nucleic acid amplification reagent containing area, and the deep hole of each area is matched with a magnetic rod sleeve fixed on the first magnetic rod sleeve seat.
In the above-mentioned nucleic acid isolation apparatus, the magnetic rod holder may further comprise a second magnetic rod holder; the second magnetic rod rack comprises a second magnetic rod seat and a plurality of magnetic rods fixed on the second magnetic rod seat; the first magnetic rod seat and the second magnetic rod seat are provided with a space and movably connected;
the magnetic rod sleeve frame can also comprise a second magnetic rod sleeve frame; the second magnetic bar sleeve frame comprises a second magnetic bar sleeve seat and a plurality of magnetic bar sleeves fixed on the second magnetic bar sleeve seat; the magnetic rod sleeve fixed on the second magnetic rod sleeve seat is matched with the magnetic rod fixed on the second magnetic rod seat;
the deep well plate may further comprise a second extraction zone; the second extraction area sequentially comprises a second lysate containing area, a second washing solution drying area and a second nucleic acid amplification reagent containing area, and the deep hole of each area is matched with a magnetic rod sleeve fixed on the second magnetic rod sleeve seat.
In the above-mentioned nucleic acid extracting apparatus, the first extracting region may further include a first waste magnetic rod sleeve disposing region and/or a first blank region after the first nucleic acid amplification reagent disposing region;
the second extraction area can also comprise a second waste magnetic rod sleeve placing area and/or a second blank area after the second nucleic acid amplification reagent placing area.
Further, the deep hole plate is a ninety-six hole deep hole plate with eight rows and twelve columns;
the number of the magnetic rods fixed on the first magnetic rod seat and the second magnetic rod seat is eight; the number of the magnetic rod sleeves fixed on the first magnetic rod sleeve seat and the second magnetic rod sleeve seat is eight;
the first column to the sixth column in the deep hole plate are sequentially a first lysis solution placing area, a first washing solution drying area, a first nucleic acid amplification reagent placing area, a first waste magnetic rod sleeve placing area and a first blank area; the seventh row to the twelfth row of the deep pore plate are sequentially the second lysate containing area, the second washing solution drying area, the second nucleic acid amplification reagent containing area, the second waste magnetic rod sleeve containing area and the second blank area.
In the above-mentioned nucleic acid isolation apparatus, each of the plurality of magnetic rods comprises a connecting portion and a magnetic end portion; the connecting part of the magnetic rod fixed on the first magnetic rod seat and the first magnetic rod seat are of an integral structure; the connecting part of the magnetic rod fixed on the second magnetic rod seat and the second magnetic rod seat are of an integral structure.
Further, the connecting part and the magnetic end part are in bonding connection.
In the above-mentioned nucleic acid isolation apparatus, the first and second magnetic rod holders are movably connected at positions located at end portions of the first and second magnetic rod holders, and the entire magnetic rod holder including the first and second magnetic rod holders has a first end portion and a second end portion.
Further, the first end portion and the second end portion are provided with handles.
In the above-mentioned nucleic acid isolation apparatus, at least one end of the first magnetic rod sleeve and at least one end of the second magnetic rod sleeve are each provided with a handle.
Furthermore, the upper surfaces of the handles of the first magnetic rod seat, the second magnetic rod seat, the first magnetic rod sleeve seat and the second magnetic rod sleeve seat can be in a wave-shaped concave-convex structure.
The utility model has the following beneficial effects:
the nucleic acid extraction device adopts the magnetic rod type nucleic acid extraction device, can overcome the defects of small flux, complex operation, long time consumption and the like of a pipetting type magnetic bead extraction method, reduces the pressure of manually extracting nucleic acid, adopts a 3D printing technology to print main body elements of the device, has simple structure and low cost, can be repeatedly utilized, adopts 8-linked magnetic rod sleeves which are low in price and can be produced in batch and a 96-pore plate, can realize that 16 samples can be extracted from a single 96-pore plate at the same time, and is concretely as follows:
(1) according to the nucleic acid extraction device, the main body of the magnetic rod rack is printed by using the white resin, the device is small and exquisite, the price is low, the magnetic rod rack can be repeatedly used, and the neodymium iron boron strong magnet is purchased, so that the cost is low, the magnetic bead adsorption effect is good, and the magnetic bead is not easy to fall off.
(2) The 8-linked magnetic rod sleeve in the nucleic acid extraction device is low in price and can be produced in large batch, and the extraction platform based on the 96-well plate is not high in price. Compared with a pipetting type magnetic bead extraction method, the method has the advantages of simple steps, high flux and less errors, and can realize that 16 samples can be extracted from a single 96-well plate at the same time.
(3) The nucleic acid extraction device simplifies the extraction steps, reduces the number of washing steps and omits the elution step compared with the traditional liquid-moving magnetic bead extraction method. The washing steps are reduced to one time, and the good washing effect is still achieved, and the CT difference between the washing once and the washing twice is within 0.5 and falls within an acceptable range.
Drawings
FIG. 1 is a schematic structural diagram of a nucleic acid extraction apparatus according to an embodiment of the present invention.
FIG. 2 is a schematic structural view of a magnetic rod holder in the nucleic acid isolation apparatus according to an embodiment of the present invention.
FIG. 3 is a schematic view showing a structure of a magnetic rod holder in the nucleic acid isolation apparatus according to still another embodiment of the present invention.
FIG. 4 is a schematic structural diagram of a deep well plate in the nucleic acid isolation apparatus according to an embodiment of the present invention.
FIG. 5 is a flow chart of nucleic acid extraction using the nucleic acid extraction apparatus according to one embodiment of the present invention.
In the figure, the respective symbols are as follows:
1-magnetic rod rack, 2-magnetic rod sleeve rack, 3-deep hole plate, 4-first magnetic rod seat, 5-magnetic rod, 6-second magnetic rod seat, 7-first magnetic rod sleeve seat, 8-magnetic rod sleeve, 9-second magnetic rod sleeve seat, 10-first lysate placing area, 11-first cleaning solution placing area, 12-first cleaning solution drying area, 13-first nucleic acid amplification reagent placing area, 14-first waste magnetic rod sleeve placing area, 15-first blank area, 16-second lysate placing area, 17-second cleaning solution placing area, 18-second cleaning solution drying area, 19-second nucleic acid amplification reagent placing area, 20-second waste magnetic rod sleeve placing area, 21-second blank area, 22-connecting part and 23-magnetic end part, 24-handle.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention are described clearly and completely below, and it is obvious that the described embodiments are some, not all embodiments of the present invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
Unless defined otherwise, technical or scientific terms used herein shall have the ordinary meaning as understood by one of ordinary skill in the art to which this invention belongs. The use of "first," "second," and similar terms in the present application do not denote any order, quantity, or importance, but rather the terms are used to distinguish one element from another. The word "comprising" or "comprises", and the like, means that the element or item listed before the word covers the element or item listed after the word and its equivalents, but does not exclude other elements or items. The terms "connected" or "coupled" and the like are not restricted to physical or mechanical connections, but may include electrical connections, whether direct or indirect.
The utility model provides a nucleic acid extraction device, which comprises a magnetic rod rack, a magnetic rod sleeve rack and a deep hole plate;
the magnetic rod rack comprises a first magnetic rod rack; the first magnetic rod rack comprises a first magnetic rod seat and a plurality of magnetic rods fixed on the first magnetic rod seat;
the magnetic bar sleeve frame comprises a first magnetic bar sleeve frame; the first magnetic bar sleeve frame comprises a first magnetic bar sleeve seat and a plurality of magnetic bar sleeves fixed on the first magnetic bar sleeve seat; the magnetic rod sleeve fixed on the first magnetic rod sleeve seat is matched with the magnetic rod fixed on the first magnetic rod seat;
the deep well plate includes a first extraction zone; the first extraction area comprises a first lysate containing area, a first washing solution drying area and a first nucleic acid amplification reagent containing area in sequence, and the deep hole of each area is matched with a magnetic rod sleeve fixed on a first magnetic rod sleeve seat.
Further, the magnetic rod rack also comprises a second magnetic rod rack; the second magnetic rod rack comprises a second magnetic rod seat and a plurality of magnetic rods fixed on the second magnetic rod seat; the first magnetic rod seat and the second magnetic rod seat are provided with a space and movably connected;
the magnetic rod sleeve frame also comprises a second magnetic rod sleeve frame; the second magnetic bar sleeve frame comprises a second magnetic bar sleeve seat and a plurality of magnetic bar sleeves fixed on the second magnetic bar sleeve seat; the magnetic rod sleeve fixed on the second magnetic rod sleeve seat is matched with the magnetic rod fixed on the second magnetic rod seat;
the deep well plate further comprises a second extraction zone; the second extraction area sequentially comprises a second lysate containing area, a second washing solution drying area and a second nucleic acid amplification reagent containing area, and the deep hole of each area is matched with a magnetic rod sleeve fixed on a second magnetic rod sleeve seat.
The nucleic acid extraction device can overcome the defects of small flux, complex operation, long time consumption and the like of a liquid-transfer magnetic bead extraction method, and reduces the pressure of manually extracting nucleic acid.
The following will describe the nucleic acid isolation apparatus of the present invention in detail by taking an eight-coupled magnetic rod set and a ninety-six well deep-well plate as examples, but the present invention is not limited to the following examples.
FIG. 1 is a view showing a nucleic acid isolation apparatus according to an embodiment of the present invention. As shown in FIG. 1, the nucleic acid extracting apparatus comprises a magnetic rod holder 1, a magnetic rod sleeve holder 2 and a deep well plate 3;
as shown in fig. 2 and 3, the magnet bar holder 1 includes a first magnet bar holder and a second magnet bar holder; the first magnetic rod rack comprises a first magnetic rod seat 4 and eight magnetic rods 5 fixed on the first magnetic rod seat; the second magnetic rod rack comprises a second magnetic rod seat 6 and eight magnetic rods 5 fixed on the second magnetic rod seat; the first magnetic rod seat 4 and the second magnetic rod seat 6 are arranged with a space and movably connected;
the magnetic bar sleeve frame 2 comprises a first magnetic bar sleeve frame and a second magnetic bar sleeve frame; the first magnetic bar sleeve frame comprises a first magnetic bar sleeve seat 7 and eight magnetic bar sleeves 8 fixed on the first magnetic bar sleeve seat; the magnetic rod sleeve fixed on the first magnetic rod sleeve seat 7 is matched with the magnetic rod 5 fixed in the first magnetic rod seat 4; the second magnetic bar sleeve frame comprises a second magnetic bar sleeve seat 9 and eight magnetic bar sleeves 8 fixed on the second magnetic bar sleeve seat 9; the magnetic rod sleeve 8 fixed on the second magnetic rod sleeve seat 9 is matched with the magnetic rod 5 fixed in the second magnetic rod seat 6;
as shown in fig. 4, the deep hole plate 3 is a ninety-six hole deep hole plate of eight rows and twelve columns, including a first extraction area and a second extraction area; the first extraction area is a first row to a sixth row of the deep-hole plate, and sequentially comprises a first lysate placing area 10, a first washing liquid placing area 11, a first washing liquid drying area 12, a first nucleic acid amplification reagent placing area 13, a first waste magnetic rod sleeve placing area 14 and a first blank area 15, and the deep holes of each area are matched with the magnetic rod sleeve 8 fixed on the first magnetic rod sleeve seat 7; the second extraction area is the seventh row to the twelfth row of the deep hole plate, and is sequentially a second lysate placing area 16, a second washing liquid placing area 17, a second washing liquid drying area 18, a second nucleic acid amplification reagent placing area 19, a second waste magnetic rod sleeve placing area 20 and a second blank area 21, and the deep holes of each area are matched with the magnetic rod sleeves 8 fixed on the second magnetic rod sleeve seats 9. The first blank area 15 and the second blank area 21 prevent cross contamination on the left and right sides.
The utility model can extract 1-8 samples or 9-16 samples simultaneously by detaching or connecting the first magnetic rod seat 4 and the second magnetic rod seat 6, thereby meeting the extraction of different fluxes. The magnetic rod holder of the nucleic acid isolation apparatus shown in FIG. 2 can be used in combination with the magnetic rod holder and the deep well plate to extract 9 to 16 samples of nucleic acids, and the magnetic rod holder of the nucleic acid isolation apparatus shown in FIG. 3, which is divided into two parts, can be used in combination with the first magnetic rod holder and the first extraction region of the deep well plate to extract 1 to 8 samples of nucleic acids.
Specifically, as shown in fig. 2 and 3, each of the plurality of magnet bars 5 is composed of a connecting portion 22 and a magnetic end portion 23, and the connecting portion 22 of the magnet bar 5 fixed to the first magnet bar holder 4 is integrated with the first magnet bar holder 4; the connecting part 22 of the magnetic rod 5 fixed on the second magnetic rod seat 6 and the second magnetic rod seat 6 are of an integral structure. The connection portion 22 and the end portion 23 having magnetic properties may be adhesively connected, such as by adhesive tape or epoxy glue. The integrated bracket can be manufactured by a 3D printing technology and can be repeatedly utilized. The magnetic end 23 can be a cylindrical neodymium iron boron magnet, such as a magnet with the magnet performance brand of N38, and has the advantages of low cost, good magnetic bead adsorption effect and difficult falling.
Specifically, as shown in fig. 1 and 2, the positions of the first magnet rod seat 4 and the second magnet rod seat 6 which are movably connected are located at the ends of the first magnet rod seat 4 and the second magnet rod seat 6 to form a U-shaped magnet rod seat; the entire magnet bar holder of the first magnet bar holder 4 and the second magnet bar holder 6 has a first end and a second end. Further, the first end portion and the second end portion are each provided with a handle 24. Further, the upper surfaces of the handles 24 of the first magnet rod seat 4 and the second magnet rod seat 6 are both in a wave-shaped concave-convex structure.
Specifically, as shown in fig. 1, at least one end of the first magnetic rod sleeve seat and at least one end of the second magnetic rod sleeve seat are provided with a handle 24. Further, the upper surfaces of the handles 24 of the first magnetic rod sleeve seat 7 and the second magnetic rod sleeve seat 9 are both in a wave-shaped concave-convex structure.
When the nucleic acid extraction device is used, whether the first magnetic rod seat 4 and the second magnetic rod seat 6 are separated or not is selected according to the number of nucleic acid samples to be detected, and nucleic acid is extracted according to a flow chart shown in fig. 5, and the specific steps are as follows:
1) preparation of reagents:
80% ethanol is prepared and placed at normal temperature (used when being prepared), then qPCR reaction solution is prepared to be used as a nucleic acid amplification reagent, and the nucleic acid amplification reagent is placed in a refrigerator at 4 ℃ for standby (the preparation is best used up in the same day). And taking out the detection sample from a refrigerator at the temperature of-80 ℃, and after melting, carrying out gradient dilution and placing for later use. The lysate can be prepared into a bottle in advance and then is subpackaged for use, the unused lysate is placed in the shade, and the lysate can be placed for a long time.
2) Cracking:
adding lysis solution, sample solution and magnetic bead solution into the first lysis solution placement area 10 and/or the second lysis solution placement area 16, inserting the first magnetic rod sleeve frame and/or the second magnetic rod sleeve frame, shaking up and down, uniformly mixing, and standing for lysis; after the cracking is finished, the magnetic rod 5 of the first magnetic rod rack and/or the second magnetic rod rack is slowly inserted into the magnetic rod sleeve 8 in the first magnetic rod sleeve rack and/or the second magnetic rod sleeve rack, and the magnetic beads are adsorbed at the bottom of the magnetic rod sleeve 8 as much as possible.
3) Washing and drying
And slowly extracting the adsorbed magnetic rod sleeve frame 2 and the magnetic rod frame 1 from the lysate, inserting the magnetic rod sleeve frame 2 and the magnetic rod frame 2 into the first cleaning solution containing area 11 and/or the second cleaning solution containing area 17, extracting the magnetic rod frame 1, shaking the magnetic rod sleeve frame 2 to uniformly disperse magnetic beads in the cleaning solution, inserting the magnetic rod 5 in the magnetic rod frame 1 into the magnetic rod sleeve 8 in the magnetic rod sleeve frame 2 to adsorb the magnetic beads after the cleaning is finished, extracting the magnetic rod sleeve frame 2 and the magnetic rod frame 1 from the cleaning solution together, and placing the magnetic rod sleeve frame 2 and the magnetic rod frame 1 in the first cleaning solution drying area 12 and/or the second cleaning solution drying area 18 for drying.
4) qPCR reaction:
the method comprises the steps of subpackaging qPCR reagents into a qPCR reaction area, namely a first nucleic acid amplification reagent placing area 13 and/or a second nucleic acid amplification reagent placing area 19 in advance, placing a magnetic rod rack 1 and a magnetic rod sleeve rack 2 into the qPCR reaction area together, drawing away the magnetic rod rack 1, shaking the magnetic rod sleeve rack 2, dispersing magnetic beads into qPCR reaction liquid, drawing out the magnetic rod sleeve rack 2, and placing the magnetic rod sleeve rack 2 into a waste magnetic rod sleeve placing area, namely a first waste magnetic rod sleeve placing area 14 and/or a second magnetic rod sleeve placing area 20. And transferring the mixed solution into a qPCR reaction tube, and setting a reaction program to start reaction. Thus, the extraction of nucleic acid can be realized.
Claims (10)
1. A nucleic acid extraction device, characterized in that: comprises a magnetic bar rack, a magnetic bar sleeve rack and a deep hole plate;
the magnetic rod rack comprises a first magnetic rod rack; the first magnetic rod rack comprises a first magnetic rod seat and a plurality of magnetic rods fixed on the first magnetic rod seat;
the magnetic bar sleeve frame comprises a first magnetic bar sleeve frame; the first magnetic bar sleeve frame comprises a first magnetic bar sleeve seat and a plurality of magnetic bar sleeves fixed on the first magnetic bar sleeve seat; the magnetic rod sleeve fixed on the first magnetic rod sleeve seat is matched with the magnetic rod fixed on the first magnetic rod seat;
the deep well plate comprises a first extraction zone; the first extraction area sequentially comprises a first lysate containing area, a first washing solution drying area and a first nucleic acid amplification reagent containing area, and the deep hole of each area is matched with a magnetic rod sleeve fixed on the first magnetic rod sleeve seat.
2. The nucleic acid extraction apparatus according to claim 1, characterized in that: the magnetic rod rack also comprises a second magnetic rod rack; the second magnetic rod rack comprises a second magnetic rod seat and a plurality of magnetic rods fixed on the second magnetic rod seat; the first magnetic rod seat and the second magnetic rod seat are provided with a space and movably connected;
the magnetic rod sleeve frame also comprises a second magnetic rod sleeve frame; the second magnetic bar sleeve frame comprises a second magnetic bar sleeve seat and a plurality of magnetic bar sleeves fixed on the second magnetic bar sleeve seat; the magnetic rod sleeve fixed on the second magnetic rod sleeve seat is matched with the magnetic rod fixed on the second magnetic rod seat;
the deep well plate further comprises a second extraction zone; the second extraction area sequentially comprises a second lysate containing area, a second washing solution drying area and a second nucleic acid amplification reagent containing area, and the deep hole of each area is matched with a magnetic rod sleeve fixed on the second magnetic rod sleeve seat.
3. The nucleic acid extraction apparatus according to claim 2, characterized in that: the first extraction area also comprises a first waste magnetic rod sleeve placing area and/or a first blank area behind the first nucleic acid amplification reagent placing area;
the second extraction area also comprises a second waste magnetic rod sleeve placing area and/or a second blank area behind the second nucleic acid amplification reagent placing area.
4. The nucleic acid extraction apparatus according to claim 3, characterized in that: the deep hole plate is a ninety-six hole deep hole plate with eight rows and twelve columns;
the number of the magnetic rods fixed on the first magnetic rod seat and the second magnetic rod seat is eight; the number of the magnetic rod sleeves fixed on the first magnetic rod sleeve seat and the second magnetic rod sleeve seat is eight;
the first column to the sixth column in the deep hole plate are sequentially a first lysis solution placing area, a first washing solution drying area, a first nucleic acid amplification reagent placing area, a first waste magnetic rod sleeve placing area and a first blank area; the seventh row to the twelfth row of the deep pore plate are sequentially the second lysate containing area, the second washing solution drying area, the second nucleic acid amplification reagent containing area, the second waste magnetic rod sleeve containing area and the second blank area.
5. The nucleic acid extraction apparatus according to claim 2, characterized in that: each of the plurality of magnetic rods is composed of a connecting part and a magnetic end part; the connecting part of the magnetic rod fixed on the first magnetic rod seat and the first magnetic rod seat are of an integral structure; the connecting part of the magnetic rod fixed on the second magnetic rod seat and the second magnetic rod seat are of an integral structure.
6. The nucleic acid extraction apparatus according to claim 5, characterized in that: the connecting part is connected with the magnetic end part in an adhesive way.
7. The nucleic acid extraction apparatus according to claim 2, characterized in that: the movable connection position of the first magnetic rod seat and the second magnetic rod seat is located at the end parts of the first magnetic rod seat and the second magnetic rod seat, and the whole magnetic rod seat formed by the first magnetic rod seat and the second magnetic rod seat is provided with a first end part and a second end part.
8. The nucleic acid extraction apparatus according to claim 7, characterized in that: the first end portion and the second end portion are both provided with handles.
9. The nucleic acid extraction apparatus according to claim 2, characterized in that: at least one end of the first magnetic rod sleeve seat and at least one end of the second magnetic rod sleeve seat are both provided with handles.
10. The nucleic acid extraction apparatus according to claim 8 or 9, characterized in that: the upper surfaces of the handles of the first magnetic rod seat, the second magnetic rod seat, the first magnetic rod sleeve seat and the second magnetic rod sleeve seat are of wavy concave-convex structures.
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| CN202121131312.8U CN215560195U (en) | 2021-05-25 | 2021-05-25 | Plain type nucleic acid extraction element |
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| CN202121131312.8U CN215560195U (en) | 2021-05-25 | 2021-05-25 | Plain type nucleic acid extraction element |
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