CN111329959A

CN111329959A - Compound traditional Chinese medicine for intervening gastric cancer infiltration and metastasis and preparation method thereof

Info

Publication number: CN111329959A
Application number: CN202010269059.6A
Authority: CN
Inventors: 李卫强; 魏雪红
Original assignee: Ningxia Medical University
Current assignee: Ningxia Medical University
Priority date: 2020-04-08
Filing date: 2020-04-08
Publication date: 2020-06-26

Abstract

The invention discloses a compound traditional Chinese medicine for intervening gastric cancer infiltration and metastasis and a preparation method thereof, wherein the compound traditional Chinese medicine comprises the following raw material medicines in parts by weight: 30-80 parts of radix pseudostellariae, 20-50 parts of dendrobium, 30-60 parts of dark plum fruit, 10-20 parts of radix bupleuri, 6-15 parts of rhizoma cimicifugae, 20-60 parts of barbed skullcap herb, 10-50 parts of combined spicebush root, 20-50 parts of lizard, 30-90 parts of astragalus mongholicus, 20-60 parts of angelica sinensis, 20-60 parts of Indian mockstrawberry herb, 30-90 parts of prepared rehmannia root, 10-30 parts of raw rehmannia root, 20-50 parts of lily, 30-60 parts of white paeony root, 5-10 parts of pseudo-ginseng powder, 20-60 parts of raw oyster shell, 20-40 parts of deerhorn glue, 20-60 parts of Chinese yam, 20-50 parts of bletilla striata, 10-30 parts of immature bitter. The invention also discloses a preparation method of the compound traditional Chinese medicine for intervening gastric cancer infiltration and metastasis. The medicine of the invention is a pure Chinese medicine preparation, and has small side effect.

Description

Compound traditional Chinese medicine for intervening gastric cancer infiltration and metastasis and preparation method thereof

Technical Field

The invention belongs to the technical field of medicines, and relates to a compound traditional Chinese medicine for intervening gastric cancer infiltration and metastasis and a preparation method thereof.

Background

The gastric cancer is one of the common clinical malignant tumors, the number of newly increased gastric cancer people in China is the top of the world every year, and the death rate of the gastric cancer people is the first three of all cancer death rates in China. In China, the incidence of gastric cancer is second place for general malignant tumors in men and fourth place for women. Research shows that the early gastric cancer with pathological changes in mucosa and submucosa can lead the 5-year survival rate of patients to reach more than 90 percent, and the metastasis is one of the main reasons influencing the curative effect and prognosis of gastric cancer patients and seriously threatens the health and life of the patients.

Because early symptoms of patients with gastric cancer are hidden, an effective screening and treating method is not available at present, the diagnosis of precancerous lesions and early cancers is also very limited, and the pathogenesis of the gastric cancer is not completely clear, so that the diagnosis, prevention and treatment and transfer inhibition of the gastric cancer become one of clinical difficulties. Currently, there is still a lack of effective intervention and therapeutic drugs for gastric cancer. The importance and the urgency of the research on the curative effect and the mechanism of the traditional Chinese medicine for resisting the tumor and inhibiting the tumor infiltration and migration are shown by actively developing the effective medicines with high efficiency and low toxicity.

The PI3K/AKT signaling pathway is closely related to the formation and development of various malignant tumors, and plays a role in the connection between signaling pathways in tumor cells. Aberrant activation of the PI3K/AKT transduction pathway or genetic mutations are common in a variety of human tumor tissues.

Research has shown that PI3K/AKT is the main signaling pathway regulating EMT, whereas HIF-1 α activation under ischemic and hypoxic conditions is the main upstream growth factor regulating PI 3K/AKT.

Disclosure of Invention

The invention aims to provide a compound traditional Chinese medicine for intervening gastric cancer infiltration and metastasis and a preparation method thereof.

The specific technical scheme is as follows:

a compound traditional Chinese medicine for intervening gastric cancer infiltration and metastasis comprises the following raw material medicines in parts by weight: 30-80 parts of radix pseudostellariae, 20-50 parts of dendrobium, 30-60 parts of dark plum fruit, 10-20 parts of radix bupleuri, 6-15 parts of rhizoma cimicifugae, 20-60 parts of barbed skullcap herb, 10-50 parts of combined spicebush root, 20-50 parts of lizard, 30-90 parts of astragalus mongholicus, 20-60 parts of angelica sinensis, 20-60 parts of Indian mockstrawberry herb, 30-90 parts of prepared rehmannia root, 10-30 parts of raw rehmannia root, 20-50 parts of lily, 30-60 parts of white paeony root, 5-10 parts of pseudo-ginseng powder, 20-60 parts of raw oyster shell, 20-40 parts of deerhorn glue, 20-60 parts of Chinese yam, 20-50 parts of bletilla striata, 10-30 parts of immature bitter.

Further, the first preferred method: 30 parts of radix pseudostellariae, 20 parts of dendrobe, 20 parts of dark plum fruit, 10 parts of radix bupleuri, 6 parts of cimicifugae foetidae, 20 parts of barbed skullcap herb, 10 parts of combined spicebush root, 20 parts of lizard, 30 parts of astragalus root, 20 parts of angelica, 20 parts of Indian mockstrawberry herb, 30 parts of prepared rehmannia root, 10 parts of raw rehmannia root, 20 parts of lily, 20 parts of white paeony root, 5 parts of pseudo-ginseng powder, 20 parts of raw oyster shell, 10 parts of deerhorn glue, 20 parts of Chinese yam, 20 parts of bletilla striata, 10 parts.

The second preferred method is as follows: 20 parts of radix pseudostellariae, 10 parts of dendrobe, 10 parts of dark plum fruit, 10 parts of radix bupleuri, 6 parts of cimicifugae foetidae, 10 parts of barbed skullcap herb, 10 parts of combined spicebush root, 10 parts of lizard, 15 parts of astragalus root, 10 parts of angelica, 10 parts of Indian mockstrawberry herb, 30 parts of prepared rehmannia root, 10 parts of raw rehmannia root, 10 parts of lily, 10 parts of white paeony root, 5 parts of pseudo-ginseng powder, 10 parts of raw oyster shell, 10 parts of deerhorn glue, 10 parts of Chinese yam, 10 parts of bletilla striata, 10 parts.

The preferable method is three: 50 parts of radix pseudostellariae, 30 parts of dendrobium, 40 parts of dark plum fruit, 15 parts of radix bupleuri, 10 parts of cimicifugae foetidae, 30 parts of barbed skullcap herb, 30 parts of combined spicebush root, 20 parts of lizard, 60 parts of astragalus root, 30 parts of angelica, 30 parts of Indian mockstrawberry herb, 60 parts of prepared rehmannia root, 20 parts of raw rehmannia root, 30 parts of lily, 40 parts of white paeony root, 6 parts of pseudo-ginseng powder, 30 parts of raw oyster shell, 30 parts of deerhorn glue, 30 parts of Chinese yam, 30 parts of bletilla striata, 15 parts of.

The preferable method is four: 50 parts of radix pseudostellariae, 20 parts of dendrobe, 30 parts of dark plum fruit, 10 parts of radix bupleuri, 6 parts of cimicifugae foetidae, 30 parts of barbed skullcap herb, 15 parts of combined spicebush root, 30 parts of lizard, 60 parts of astragalus root, 30 parts of angelica, 30 parts of Indian mockstrawberry herb, 60 parts of prepared rehmannia root, 15 parts of dried rehmannia root, 30 parts of lily, 30 parts of white paeony root, 6 parts of pseudo-ginseng powder, 20 parts of raw oyster shell, 30 parts of deerhorn glue, 60 parts of Chinese yam, 20 parts of bletilla striata, 10 parts.

The invention relates to a preparation method of a compound traditional Chinese medicine for intervening gastric cancer infiltration and metastasis,

step 1, after the raw materials are subjected to purification treatment such as screening to remove impurities, washing with distilled water to remove dust for 3 times and the like, drying for 2-4 hours in an oven to remove water until the medicine turns yellowish.

And 2, carrying out water-grinding on bletilla striata in the raw material medicines, appropriately crushing the bletilla striata, placing the crushed bletilla striata in a mortar, adding an appropriate amount of distilled water, grinding the mixture into paste, adding a large amount of distilled water, stirring, pouring out a suspension after the coarse particle substances sink, grinding the settled coarse particle substances again, and repeating the operation for 3 times until the bletilla striata is ground into fine powder. Mixing the above suspensions, standing, precipitating, removing supernatant, and grinding into superfine powder.

Step 3, distilling and cleaning lizards, airing, smashing and homogenizing by a pounder, soaking for 24 hours, and performing suction filtration; soaking in 95% ethanol solution for 12 hr, repeating for 4 times, mixing filtrates, rotary evaporating, concentrating, removing ethanol, rotary evaporating the remaining water suspension, concentrating, freeze drying, and grinding into fine powder.

And 4, adopting a pulverizer for the rest medicines, then respectively sieving the medicines with a sieve of 80 meshes and a sieve of 100 meshes, respectively taking the medicines sieved with the sieve of 80 meshes and the medicines sieved with the sieve of 100 meshes, uniformly mixing the medicines with the sieve of 80 meshes and the medicines sieved with the sieve of 100 meshes in equal proportion, and adding fine powder of bletilla striata and lizard for later use.

Compared with the prior art, the invention has the beneficial effects that:

the medicine is a pure Chinese medicine preparation, has small side effect, inhibits cell proliferation and promotes cell apoptosis by influencing the expression of PI3K/AKT protein, and has better effect of resisting gastric cancer infiltration and transfer.

Drawings

FIG. 1 shows OD values of each group of the compound Chinese medicinal preparation after the rat serum containing the medicine is dried;

fig. 2 is a graph showing the change of apoptosis rate of each gastric cancer cell in the drug-containing serum intervention culture, wherein fig. 2A is a physiological saline blank control group, fig. 2B is a 5-FU dry pre-group, fig. 2C is a cinobufagin tablet dry pre-group, fig. 2D is a lizard preparation 80 mesh particle dry pre-group, fig. 2E is a lizard preparation 100 mesh particle dry pre-group, and fig. 2F, fig. 2G and fig. 2H are lizard preparation 80 mesh +100 mesh equal-ratio mixed particle high, medium and low dose dry pre-groups, respectively;

FIG. 3 shows the effect of drug-containing rat serum on gastric cancer cell apoptosis;

FIG. 4 shows the total protein expression of p-AKT, AKT and TGF- β for each treatment group, wherein FIG. 4a shows WB detecting p-AKT, AKT and TGF- β expression, FIG. 4b shows p-AKT and AKT expression for each treatment group, and FIG. 4c shows TGF- β protein expression for each treatment group.

Detailed Description

The technical solution of the present invention will be described in further detail with reference to specific examples.

1. The compound traditional Chinese medicine preparation contains drug-containing rat serum which has the following effects on the proliferation of human gastric cancer cells: as shown in fig. 1.

Grouping description: respectively blank control group, 5-FU dry preparation group, cinobufagin tablet dry preparation group, Eremiatis Argi preparation 80 mesh granule group, 100 mesh granule group, 80 mesh +100 mesh group, etc. with higher, medium and low dosage.

① compared with the blank control group, after the serum of each group of rats containing the drug acts on gastric cancer cells, the cell proliferation activity is obviously inhibited (P is less than 0.05).

② the OD value of each group of the compound Chinese medicinal preparation is less than that of cinobufagin and 5-FU positive control group (P is less than 0.05).

③ the compound Chinese medicinal preparation is sieved with 80 mesh sieve and 100 mesh sieve, and mixed with low, medium and high dose groups (with OD mean value less than 80 mesh and 100 mesh) respectively.

2. The compound traditional Chinese medicine preparation contains drug-containing rat serum which has the following effects on the apoptosis of human gastric cancer cells: see fig. 2A-2H, fig. 3.

① compared with the control group, the apoptosis of each group of rat serum containing the medicine is obvious after the serum acts on MGC-803 gastric cancer cells (P is less than 0.05).

② the apoptosis rate of each group of the compound Chinese medicinal preparation is higher than that of cinobufagin and 5-FU positive drug control group (P is less than 0.05).

③ the compound Chinese medicinal preparation has low, medium and high dose of 80-mesh and 100-mesh mixed granules with higher apoptosis rate than simple 80-mesh and 100-mesh groups, as shown in figure 2F (P is less than 0.05).

Fig. 2 is a graph showing the change of apoptosis rate of each gastric cancer cell in the drug-containing serum intervention culture, fig. 2A is a physiological saline blank control group, fig. 2B is a 5-FU dry pretreatment group, fig. 2C is a cinobufagin tablet dry pretreatment group, fig. 2D is a lizard preparation 80 mesh particle dry pretreatment group, fig. 2E is a lizard preparation 100 mesh particle dry pretreatment group, and fig. 2F, fig. 2G and fig. 2H are lizard preparation 80 mesh +100 mesh equal ratio mixed particle high, medium and low dose dry pretreatment groups, respectively.

Western-blot detection of gastric cancer cell total AKT, p-AKT and TGF- β expression is shown in FIG. 4 a-FIG. 4c.

① compared with the blank group, the P-AKT and TGF- β protein expression of each drug-containing serum treatment group is reduced, P is less than 0.05, and is shown in figure 4a, figure 4b and figure 4c.

② 80 mesh group and 100 mesh group, P is more than 0.05 compared with cinobufagin, 5-FU positive drug control group P-AKT and TGF- β protein expression, as shown in figure 4a.

③ the compound preparation has P less than 0.05 compared with cinobufagin, 5-FU positive drug control group P-AKT and TGF- β protein expression in high, medium and low dose groups.

④ the compound Chinese medicinal preparation has 80 mesh and 100 mesh mixed high dose of total p-AKT, AKT and TGF- β white level reduced most obviously, as shown in fig. 4b and 4c.

Through clinical, experimental verification and theoretical discussion of various optimal formulas, the four optimal formulas are optimized.

According to the literature and the clinical case study, the main pathogenesis of the gastric cancer mainly lies in the stomach qi and yin injury, pathogenic toxin retention, blood vessel stasis and accumulation after a long time, namely the gastric cancer. The local prescription play also points out: long accumulation, blood consumption and dry and lusterless stomach. It is luggy above the throat, near the throat, feasible for water drinking, difficult to drink food, intermittent or not much, so its name is choking. It is so deep below the stomach that it is difficult to enter the stomach and recover well for a long time even if it is eaten, so its name is diaphragmatic and regurgitation. Big constipation is less, if the sheep is mild, although the name is different, the disease is integrated. Zhang's Yi Tong's records that: ancient people who choke diaphragm means that body fluid is dry and dry, so water is feasible, dry substance is unsmooth, and is lusterless and upper-jiao, and therefore people are fooled into doubt. How to eat the food and swallow the food until the fruit is withered, and how to let the saliva gush upward? Therefore, qi failing to descend between diaphragm and pharynx is the ascending of pulses and the descending of adverse qi, which indicates that the property of stomach failing to moisten and descend is the important mechanism of stomach cancer.

Therefore, on the basis of treatment according to the syndrome, the invention takes the property of stomach-qi descending to moisten and nourish stomach, detoxify and dredge collaterals into consideration, and adopts the following formula: 50 parts of radix pseudostellariae, 20 parts of dendrobium, 30 parts of dark plum fruit, 10 parts of radix bupleuri, 6 parts of cimicifugae foetidae, 30 parts of barbed skullcap herb, 15 parts of combined spicebush root, 30 parts of lizard, 60 parts of astragalus root, 30 parts of angelica, 30 parts of Indian mockstrawberry herb, 60 parts of prepared rehmannia root, 15 parts of dried rehmannia root, 30 parts of lily, 30 parts of white paeony root, 6 parts of pseudo-ginseng powder, 20 parts of raw oyster shell, 30 parts of deerhorn glue, 60 parts of Chinese yam, 20 parts of bletilla striata, 10 parts of immature bitter orange, 5 parts.

In the recipe, Eremias mandibulae, recorded in "Chinese materia Medica of medicinal animals", belongs to the species of Eremias in desert grassland, and has salty and cold taste, and enters heart and liver meridians. Has the effects of breaking knots, dissipating blood stasis, nourishing blood, promoting blood circulation, clearing heat and removing toxicity, and is recorded in compendium of materia medica: the decoction can be used for treating yin-deficiency, stopping bleeding, removing blood stasis, removing toxic materials, and preventing cancer; experimental research shows that the exendin nubilalis can promote apoptosis of BCG-823 cells of human gastric cancer, intervene expression levels of P53, Bax, matrix metalloproteinase-9 (MMP-9) and E-cad protein, and effectively block infiltration and metastasis of tumors. 50 parts of radix pseudostellariae and 60 parts of astragalus membranaceus are used in large amounts, and are used for tonifying middle-jiao energy, promoting digestion and nourishing mucous membrane. 30 parts of barbed skullcap herb and Indian mockstrawberry herb respectively, heavy dose, and the medicines for removing toxin, dredging collaterals, promoting blood circulation, and clearing away damp-heat; the stomach is moist and aversion to dryness, 20 parts of dendrobium, 30 parts of dark plum fruit and 30 parts of white paeony root have the effects of nourishing yin, promoting the production of body fluid, harmonizing stomach, softening liver and relieving pain. 10 portions of immature bitter orange, fructus Aurantii Immaturus promote the circulation of qi, purge fu-organs and relieve distension, prevent yin-nourishing herbs from nourishing greasy and stagnated stomach. The whole formula has the effects of tonifying qi and yin, and generating and transforming qi and blood, and can dissipate toxic stasis, nourish mucous membrane and block canceration. The experimental research also shows that the method can inhibit cell proliferation and promote cell apoptosis by influencing the expression of P53 and Bax proteins, and has better anticancer effect.

[ classic case ] A certain sea, male, 70 years old, first diagnosis in 2017, 10 and 11 days. The main complaints are: the dull pain in the epigastrium is aggravated for 1 week after three months. Before March, patients feel dull pain in the stomach cavity due to family trouble, accompanied by dry mouth, slight bitterness in the morning, acid regurgitation and good stool and stool. Gastroscopy in local hospitals indicates that chronic atrophic gastritis is accompanied with erosion, the pathology indicates that gastric mucosa is heavily hyperplastic (namely in situ cancer), patients do not want to perform surgery and adopt medicine conservative treatment, and pain is gradually relieved. Before 1 week, the patient is out of the clinic because of the aggravated stomachache due to the emotional disorder. The symptoms are as follows: dull pain in the epigastrium, dry mouth, slight bitterness in the morning, occasional acid regurgitation, expression stagnation, facial complexion discomfort, pale and dark tongue with fissures, white and greasy coating, partial peeling, and wiry and thready pulse. The syndrome belongs to: deficiency of both qi and yin and toxic stasis, therapeutic method: supplementing qi and nourishing yin, and removing toxic substance and dredging collaterals, wherein according to the fourth scheme, after the decoction is steamed for two hours, 9g of lotus root starch is added and 10g of lotus root starch is added each time, and the mixture is mixed with boiled water to be prepared into paste to be taken with water 3 times a day. After conditioning for three months, the patient is treated with gastroscopy and shows that: chronic superficial gastritis indicates chronic mucosal inflammation and in-situ cancer hyperplasia disappears.

The compound traditional Chinese medicine preparation is clinically applied to treat patients with in-situ gastric cancer deficiency of qi and yin and toxic stasis cross-resistance, and the safety and adverse reactions of the patients are observed. The method collects 89 cases of the key department outpatient service of spleen and stomach diseases of national traditional Chinese medicine administration of Ningxia medical university affiliated to Hui medicine Hospital from 1 month to 12 months in 2014, the patients with severe intestinal hyperplasia are randomly divided into 45 cases and 44 cases of a control group by gastroscope and pathological confirmation, and the patients are identified as in-situ stomach cancer with deficiency of both qi and yin and toxin stasis cross-resistance by traditional Chinese medicine differentiation, wherein the treatment group adopts the compound traditional Chinese medicine preparation (Ningxia Midian Ereming, radix pseudostellariae, dendrobe, dark plum, radix bupleuri, cimicifugae foetidae, sculellaria barbata, combined spicebush root, lizard, radix astragali, angelica sinensis, mock strawberry, prepared rehmannia root, radix rehmanniae, lily, white paeony root, pseudo-ginseng powder, raw oyster shell, deerhorn glue, Chinese yam, bletilla striata, immature bitter orange, rhubarb and the like, and the like are provided by a preparation room affiliated to Ningxia medical university affiliated to the traditional Chinese medicine hospital preparation room; the control group orally administered stomach nourishing granule 2 bags each time for 3 times per day. Each group was taken for 12 weeks, and after the treatment course was over, two groups of clinical efficacy evaluations were performed. As a result: the total effective rate of the treatment group is 93.33 percent, the total effective rate of the control group is 72.73 percent, and the comparison P of the two groups is less than 0.05. The follow-up finds that the long-term curative effect of the treatment group is better, and no adverse reaction is caused. And (4) conclusion: the compound Chinese medicinal preparation based on syndrome research can effectively nourish gastric cancer mucosa in situ, relieve intestinal hyperplasia, kill Helicobacter Pylori (HP), and has good effect of resisting cancer infiltration and metastasis.

The effect and mechanism of the drug-containing SD rat serum on the gastric cancer cell proliferation and infiltration transfer related factor PI3K/AKT expression level of the drug-containing SD rat serum are studied by studying the effect of the drug-containing SD rat serum on the gastric cancer cell proliferation and infiltration transfer related factor PI3K/AKT expression level of the drug-containing SD rat serum. The method comprises the following steps: 120 male SPF-grade SD rats were selected for random division into 5 groups: a normal saline negative control group, a lizard stomach rehabilitation prescription high dose group, a medium dose group, a low dose group, a particle group respectively sieved by 80 meshes and 100 meshes, a cinobufagin tablet positive control group, and 15 mice in each group. After each group is respectively intervened by corresponding medicines for 1 week, blood is taken from abdominal aorta, centrifugation is carried out, medicine-containing serum is prepared, the medicine-containing serum is added to MKN-485 gastric cancer cells of human and cultured for 24 hours, and the cell proliferation is detected by a CCK-8 method; RT-PCR detects the expression of gastric cancer cell PI 3K/AKT. As a result: 1. the absorbance (OD value) of each drug-containing serogroup is less than that of a normal saline negative control group (P < 0.05); the OD value of each group of the prescription is smaller than that of a cinobufagin positive control group (P < 0.05); the OD value of each group is compared, and the effect is optimal (P <0.05) in the high-dose group. 2. The PI3K/AKT protein expression of each drug-containing serogroup is lower than that of a normal saline negative control group (P < 0.05); the PI3K/AKT protein expression of each group of the prescription is lower than that of a cinobufagin positive control group (P < 0.05); the PI3K/AK protein expression of each group of the recipe is compared, and the expression of the group with high dose is the lowest (P < 0.05). And (4) conclusion: the lizard stomach rehabilitation formula can inhibit cell proliferation and promote cell apoptosis by influencing PI3K/AKT protein expression, and has a good effect of resisting gastric cancer infiltration and metastasis.

The above description is only a preferred embodiment of the present invention, and the scope of the present invention is not limited thereto, and any simple modifications or equivalent substitutions of the technical solutions that can be obviously obtained by those skilled in the art within the technical scope of the present invention are within the scope of the present invention.

Claims

1. The compound traditional Chinese medicine for intervening gastric cancer infiltration and metastasis is characterized by comprising the following raw material medicines in parts by weight: 30-80 parts of radix pseudostellariae, 20-50 parts of dendrobium, 30-60 parts of dark plum fruit, 10-20 parts of radix bupleuri, 6-15 parts of rhizoma cimicifugae, 20-60 parts of barbed skullcap herb, 10-50 parts of combined spicebush root, 20-50 parts of lizard, 30-90 parts of astragalus mongholicus, 20-60 parts of angelica sinensis, 20-60 parts of Indian mockstrawberry herb, 30-90 parts of prepared rehmannia root, 10-30 parts of raw rehmannia root, 20-50 parts of lily, 30-60 parts of white paeony root, 5-10 parts of pseudo-ginseng powder, 20-60 parts of raw oyster shell, 20-40 parts of deerhorn glue, 20-60 parts of Chinese yam, 20-50 parts of bletilla striata, 10-30 parts of immature bitter.

2. The compound traditional Chinese medicine for intervening gastric cancer infiltration and metastasis according to claim 1, is characterized by comprising the following raw material medicines in parts by weight: 30 parts of radix pseudostellariae, 20 parts of dendrobe, 20 parts of dark plum fruit, 10 parts of radix bupleuri, 6 parts of cimicifugae foetidae, 20 parts of barbed skullcap herb, 10 parts of combined spicebush root, 20 parts of lizard, 30 parts of astragalus root, 20 parts of angelica, 20 parts of Indian mockstrawberry herb, 30 parts of prepared rehmannia root, 10 parts of raw rehmannia root, 20 parts of lily, 20 parts of white paeony root, 5 parts of pseudo-ginseng powder, 20 parts of raw oyster shell, 10 parts of deerhorn glue, 20 parts of Chinese yam, 20 parts of bletilla striata, 10 parts.

3. The compound traditional Chinese medicine for intervening gastric cancer infiltration and metastasis according to claim 1, is characterized by comprising the following raw material medicines in parts by weight: 20 parts of radix pseudostellariae, 10 parts of dendrobe, 10 parts of dark plum fruit, 10 parts of radix bupleuri, 6 parts of cimicifugae foetidae, 10 parts of barbed skullcap herb, 10 parts of combined spicebush root, 10 parts of lizard, 15 parts of astragalus root, 10 parts of angelica, 10 parts of Indian mockstrawberry herb, 30 parts of prepared rehmannia root, 10 parts of raw rehmannia root, 10 parts of lily, 10 parts of white paeony root, 5 parts of pseudo-ginseng powder, 10 parts of raw oyster shell, 10 parts of deerhorn glue, 10 parts of Chinese yam, 10 parts of bletilla striata, 10 parts.

4. The compound traditional Chinese medicine for intervening gastric cancer infiltration and metastasis according to claim 1, is characterized by comprising the following raw material medicines in parts by weight: 50 parts of radix pseudostellariae, 30 parts of dendrobium, 40 parts of dark plum fruit, 15 parts of radix bupleuri, 10 parts of cimicifugae foetidae, 30 parts of barbed skullcap herb, 30 parts of combined spicebush root, 20 parts of lizard, 60 parts of astragalus root, 30 parts of angelica, 30 parts of Indian mockstrawberry herb, 60 parts of prepared rehmannia root, 20 parts of raw rehmannia root, 30 parts of lily, 40 parts of white paeony root, 6 parts of pseudo-ginseng powder, 30 parts of raw oyster shell, 30 parts of deerhorn glue, 30 parts of Chinese yam, 30 parts of bletilla striata, 15 parts of.

5. The compound traditional Chinese medicine for intervening gastric cancer infiltration and metastasis according to claim 1, is characterized by comprising the following raw material medicines in parts by weight: 50 parts of radix pseudostellariae, 20 parts of dendrobe, 30 parts of dark plum fruit, 10 parts of radix bupleuri, 6 parts of cimicifugae foetidae, 30 parts of barbed skullcap herb, 15 parts of combined spicebush root, 30 parts of lizard, 60 parts of astragalus root, 30 parts of angelica, 30 parts of Indian mockstrawberry herb, 60 parts of prepared rehmannia root, 15 parts of dried rehmannia root, 30 parts of lily, 30 parts of white paeony root, 6 parts of pseudo-ginseng powder, 20 parts of raw oyster shell, 30 parts of deerhorn glue, 60 parts of Chinese yam, 20 parts of bletilla striata, 10 parts.

6. The preparation method of the compound traditional Chinese medicine for intervening gastric cancer infiltration and metastasis of claim 1, which is characterized by comprising the following steps:

step 1, after the raw material medicines of claim 1 are subjected to purification treatment of removing impurities by screening and washing dust by distilled water for 3 times, drying for 2-4 hours in an oven to remove water until the medicines turn to yellowish;

2, carrying out water-grinding on bletilla striata in the raw material medicines, appropriately crushing the bletilla striata, placing the crushed bletilla striata in a mortar, adding an appropriate amount of distilled water, grinding the mixture into paste, adding a large amount of distilled water, stirring, pouring out a suspension after the coarse particle substances sink, grinding the settled coarse particle substances again, and repeating the operation for 3 times until the bletilla striata is ground into fine powder; mixing the above suspensions, standing, precipitating, removing supernatant, and grinding the dried precipitate into superfine powder;

step 3, distilling and cleaning lizards, airing, smashing and homogenizing by a pounder, soaking for 24 hours, and performing suction filtration; soaking in 95% ethanol solution for 12 hr, repeating for 4 times, mixing filtrates, rotary evaporating, concentrating, removing ethanol, rotary evaporating the remaining water suspension, concentrating, freeze drying, and grinding into fine powder;

and 4, adopting a pulverizer for the rest medicines, respectively sieving the medicines with a sieve of 80 meshes and a sieve of 100 meshes, uniformly mixing the medicines with the sieve of 100 meshes in equal proportion, and adding fine powder of bletilla striata and lizard for later use.