CN111316941A - Water-regulating disease-preventing bio-base for breeding freshwater breeding animal seedlings - Google Patents

Water-regulating disease-preventing bio-base for breeding freshwater breeding animal seedlings Download PDF

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CN111316941A
CN111316941A CN202010138984.5A CN202010138984A CN111316941A CN 111316941 A CN111316941 A CN 111316941A CN 202010138984 A CN202010138984 A CN 202010138984A CN 111316941 A CN111316941 A CN 111316941A
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percent
water
base
water body
seedlings
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巩华
陈总会
江小燕
孙承文
赖迎迢
陶家发
赵长臣
刘春花
黄志斌
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Pearl River Fisheries Research Institute CAFS
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Pearl River Fisheries Research Institute CAFS
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00—Culture of aquatic animals
    • A01K61/10—Culture of aquatic animals of fish
    • A01K61/13—Prevention or treatment of fish diseases
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/66—Treatment of water, waste water, or sewage by neutralisation; pH adjustment
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/347—Use of yeasts or fungi
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/16—Nitrogen compounds, e.g. ammonia
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/16—Nitrogen compounds, e.g. ammonia
    • C02F2101/163—Nitrates
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/16—Nitrogen compounds, e.g. ammonia
    • C02F2101/166—Nitrites
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2305/00—Use of specific compounds during water treatment
    • C02F2305/06—Nutrients for stimulating the growth of microorganisms
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y02A40/818—Alternative feeds for fish, e.g. in aquacultures

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Abstract

The invention relates to a water-regulating disease-preventing bio-base for cultivating seedlings of freshwater aquaculture animals, which consists of fermented soybean meal, cane sugar molasses, wheat bran, lactic acid bacteria, saccharomycetes, monopotassium phosphate, monocalcium phosphate, magnesium sulfate and sodium chloride; wherein the lactobacillus is obtained by mixing Lactobacillus plantarum and Bacillus coagulans. The biological culture medium can be used regularly to gradually culture beneficial flora such as ammonifying bacteria, nitrosobacteria, nitrobacteria, denitrifying bacteria and the like in a water body, has stable and efficient denitrification capability, has assimilation and dissimilation functions (enabling waste nitrogen in water to leave the water body in a nitrogen form), cleans the water body, and stabilizes the system. In addition, beneficial microorganisms such as yeast and the like can be cultured, the conversion of water substances is promoted, the biomass of water zooplankton is improved, the growth of bacteria and algae is balanced, the ingestable nutrition of seedlings is increased, and the occurrence of diseases is reduced.

Description

Water-regulating disease-preventing bio-base for breeding freshwater breeding animal seedlings
Technical Field
The invention belongs to the technical field of aquaculture, and particularly relates to a water-regulating disease-preventing bio-base for cultivating seedlings of freshwater aquaculture animals.
Background
In order to improve the survival rate and the quality of the fry, two effective ways are provided for reducing the environmental stress and the nutritional stress. On one hand, water is thoroughly disinfected, and during the process of raising fry after the fry is opened and fed with bait, fresh algae liquid and the like are directionally cultured by applying culture technologies such as nitrogen fertilizer and the like to maintain algae phases, so that pathogens are effectively inhibited, and sediments are degraded; on the other hand, proper and sufficient initial feed is provided at each stage and time of the growth of the larvae, the nutrition stress on the growth of the larvae is reduced to the maximum extent, and the large and stable supply of rotifers, cladocerans and copepods is ensured. Zooplankton such as rotifer, brachypodium, copepod and the like are ingested by the fry to generate a large amount of metabolic waste such as ammonia nitrogen and the like, and the waste is discharged out of the pond by changing water, so that not only is an effective nitrogen source wasted, but also the operation cost is improved, and great pressure is brought to the environment.
In the prior production, the common practice keeps the water quality by a large amount of water feeding and discharging and water body disinfection, or adjusts the water body by common probiotics and the like, for example, a preparation method of composite heterotrophic nitrobacteria water quality improvement solid powder or granular formulation (patent publication No. CN101565239A), a screening method of photosynthetic bacteria with the capability of reducing ammonia nitrogen (patent publication No. CN104388360A), a pond micro-organism maintenance technology (patent publication No. CN106635893A), an application method of a bioremediation technology in aquaculture (patent publication No. CN101703028A), a preparation method of a water quality improvement micro-ecological preparation for reducing ammonia nitrogen and nitrite nitrogen (patent publication No. CN101565240A), a micro-organism maintenance agent for regional aquaculture and a preparation method thereof (patent publication No. 2016110697938) and the like, these methods have promoted the development of high-yield to some extent, but lack special requirements for the period of seedling cultivation.
On the other hand, the mass culture of microalgae in production plays a key role in serving as a water regulator to maintain a healthy algae phase and serving as food of opening baits such as rotifers and the like to provide sufficient nutrition. In the seedling culture season, the cultivation of rotifers is influenced by the supply and the nutritional quality of bait microalgae, the production of the bait microalgae is directly restricted by the temperature, the illumination and the variety, and the nutritional ingredients are related to the conditions of algae seeds, a culture method, storage and the like. Because of the lack of systematic research on nutritional requirements for offspring seeds, it is generally considered that microalgae fed with rotifers is high-quality microalgae rich in PUFA (EPA, DHA, and ARA), trace elements (such as Zn, Mn, Co), and the like. The conventional feeding of rotifers has incomplete nutrient substances and low nutrient value, and requires a rotifer special nutrient enrichment technology to enrich microalgae nutrition and the like. These materials are generally deposited mostly in organic debris by the food chain and do not participate effectively in the circulation of the material.
The technology adopts the excellent microorganisms screened from aquaculture, on one hand, the technology promotes the L-lactic acid generated by lactic acid bacteria to reduce the pH value, inhibit harmful bacteria and promote the growth and reproduction of the original beneficial bacteria in the water body through the carbon-nitrogen balance effect. The composite preparation such as saccharomycetes and the like is put into the aquaculture water body, so that not only mass propagation and growth of bait organisms are promoted, but also residual fish bait, excrement and organic matters in water can be decomposed, the water body environment is improved, propagation and growth of harmful bacteria in the water body are inhibited, algal balance is adjusted, and harmful bacteria and algae are controlled. The use of the microecologics mainly containing lactic acid bacteria can effectively reduce the content of ammonia nitrogen and nitrite in water; the bacillus has obvious decomposition effect on ammoniacal nitrogen and organic matters in the water body, can reduce the contents of ammoniacal nitrogen and nitrite in the water body, avoids the defects of high oxygen consumption, damage, water quality pollution and high morbidity of the traditional fertilizer, and solves the problems of detritus and feed retention in the breeding water body; the saccharomycetes can improve the microbial nutrition of the water body, can cooperatively grow with the lactic acid bacteria, realizes the reutilization of the bait, and has the effects of purifying the water quality, reducing the water change amount, saving the feed, improving the survival rate of the cultured objects, increasing the yield and the like.
Disclosure of Invention
In view of the defects of the prior art, the invention aims to provide a water-adjusting disease-preventing biological medium for cultivating seedlings of freshwater aquiculture animals, in particular to a water-adjusting disease-preventing biological medium which is regularly used in the seedling cultivation period and can gradually cultivate beneficial flora such as ammonifying bacteria, nitrosating bacteria, nitrifying bacteria, denitrifying bacteria and the like in a water body, has stable and efficient 'denitrification' capability, has assimilation and dissimilation effects (enabling waste nitrogen in water to leave the water body in the form of nitrogen), cleans the water body and stabilizes the system. In addition, beneficial microorganisms such as yeast and the like can be cultured, the conversion of water substances is promoted, the biomass of water zooplankton is improved, the growth of bacteria and algae is balanced, the edible nutrition of seedlings is increased, and the occurrence of diseases is reduced.
In order to achieve the purpose, the invention adopts the following technical scheme:
a water-regulating disease-preventing bio-base for cultivating seedlings of freshwater aquaculture animals comprises fermented soybean meal, cane sugar molasses, wheat bran, lactic acid bacteria, saccharomycetes, monopotassium phosphate, monocalcium phosphate, magnesium sulfate and sodium chloride; wherein the lactobacillus is obtained by mixing Lactobacillus plantarum and Bacillus coagulans.
Preferably, the water-regulating disease-preventing bio-base comprises the following components in percentage by mass: 60 to 80 percent of fermented soybean meal, 5 to 15 percent of cane sugar molasses, 7 to 20 percent of wheat bran, 0.25 to 1 percent of lactobacillus plantarum, 1 to 5 percent of bacillus coagulans, 0.5 to 2 percent of saccharomyces cerevisiae, 0.15 to 0.5 percent of potassium dihydrogen phosphate, 0.15 to 0.5 percent of calcium dihydrogen phosphate, 0.1 to 0.3 percent of magnesium sulfate and 0.1 to 0.3 percent of sodium chloride.
Preferably, the water-regulating disease-preventing bio-base comprises the following components in percentage by mass: 72 percent of fermented soybean meal, 8 percent of cane sugar molasses, 14 percent of wheat bran, 0.5 percent of lactobacillus plantarum, 3 percent of bacillus coagulans, 1.5 percent of beer yeast, 0.4 percent of monopotassium phosphate, 0.4 percent of calcium dihydrogen phosphate, 0.1 percent of magnesium sulfate and 0.1 percent of sodium chloride.
The invention has the following beneficial effects:
1. stimulating the growth of the original denitrification beneficial microorganism population in the water body, directionally culturing the microorganism flora in the water body, and adjusting the dynamic balance of the water body flora, phytoplankton and zooplankton.
2. By adjusting the carbon-nitrogen balance in the water body, the problem of exceeding the standard of ammonia nitrogen, nitrite and nitrate is effectively prevented and treated, stable and efficient beneficial microorganisms are directionally cultured, the self-purification capacity of the water body is improved, and the productivity is improved.
3. The material metabolism in the seedling cultivation process is promoted, the growth of the material is promoted by the material metabolism and the added bait algae, the growth of microorganisms such as yeast and the like is promoted, the accumulation of water nutrient substances to organisms such as rotifers and the like is improved, and the bait organisms with higher quality and lower cost are improved for the seedlings.
4. The pH value is stabilized, the water environment is stabilized, the ecological system is stabilized, and the occurrence of diseases is reduced.
Detailed Description
The present invention will be further described below, and it should be noted that the following examples are provided to illustrate the detailed embodiments and specific procedures based on the technical solution, but the scope of the present invention is not limited to the examples.
The invention relates to a water-regulating disease-preventing bio-base for cultivating seedlings of freshwater aquaculture animals, which consists of fermented soybean meal, cane sugar molasses, wheat bran, lactic acid bacteria, saccharomycetes, monopotassium phosphate, monocalcium phosphate, magnesium sulfate and sodium chloride; wherein the lactobacillus is obtained by mixing Lactobacillus plantarum and Bacillus coagulans.
Preferably, the water-regulating disease-preventing bio-base comprises the following components in percentage by mass: 60 to 80 percent of fermented soybean meal, 5 to 15 percent of cane sugar molasses, 7 to 20 percent of wheat bran, 0.25 to 1 percent of lactobacillus plantarum, 1 to 5 percent of bacillus coagulans, 0.5 to 2 percent of saccharomyces cerevisiae, 0.15 to 0.5 percent of potassium dihydrogen phosphate, 0.15 to 0.5 percent of calcium dihydrogen phosphate, 0.1 to 0.3 percent of magnesium sulfate and 0.1 to 0.3 percent of sodium chloride.
Preferably, the water-regulating disease-preventing bio-base comprises the following components in percentage by mass: 72 percent of fermented soybean meal, 8 percent of cane sugar molasses, 14 percent of wheat bran, 0.5 percent of lactobacillus plantarum, 3 percent of bacillus coagulans, 1.5 percent of beer yeast, 0.4 percent of monopotassium phosphate, 0.4 percent of calcium dihydrogen phosphate, 0.1 percent of magnesium sulfate and 0.1 percent of sodium chloride.
Example 1
The operation sites of this embodiment are: 3-mouth cultivation pond of Yangshaoguan Yangxing farm
In the practical use case, the water-regulating disease-preventing bio-base comprises the following components in percentage by mass: 72 percent of fermented soybean meal, 8 percent of cane sugar molasses, 14 percent of wheat bran, 0.5 percent of lactobacillus plantarum, 3 percent of bacillus coagulans, 1.5 percent of beer yeast, 0.4 percent of monopotassium phosphate, 0.4 percent of calcium dihydrogen phosphate, 0.1 percent of magnesium sulfate and 0.1 percent of sodium chloride.
In the practical use case, the application of the water diversion disease prevention bio-base in the grass carp fry rough pond is as follows:
sequentially adding water into a 5 mu cultivation pond to a depth of more than 1.5 m, adding quicklime, disinfectant, calcium chloride and magnesium sulfate to keep the pH value of the cultivation pond at 7.8-8.0, keeping the total alkalinity at 120-200mg/L, keeping the calcium hardness at 70-100mg/L, keeping the ammonia nitrogen concentration below 0.2mg/L and the nitrite concentration below 0.02mg/L, adjusting the water quality index for the second day, respectively adding the chlorella culture solution and the water-adjusting disease-preventing bio-base into the cultivation water body, and adjusting the water volume to 5 × 10-5Splashing the water in the whole pond (namely the inoculation amount of floccule seeds in each cubic meter of aquaculture water is 20 kilograms), observing that the water color is changed from light green to thick every other day according to 1 × 10 of the volume of the water body-5The bio-based of this example was added to the water for 5 consecutive days, each timeMonitoring the index change day by day until the transparency is about 0.3m-0.5m and the ammonia nitrogen concentration is below 0.2 mg/L; when the nitrite concentration is below 0.02mg/L, 20 thousands of grass carp fries with the specification of 1cm (namely 40 fries are put in each cubic water body), and the product is splashed in the whole pool every 5 days, so that the transparency is about 0.3 m.
After 12 weeks of culture, the grass carp fingerlings grow well, the body length is about 10cm, the bait coefficient is 1.4, the survival rate is 95%, the specifications are tidy, the body color and the body shape are normal, the pH value of the water body is stable, and the contents of ammonia nitrogen, nitrite and nitrate do not reach the standard exceeding level. The whole process has no large-scale epidemic disease, blue-green algae breeding and parasites, and solves the problems of the prior art that the bait coefficient is 1.6, the survival rate is 80-85%, the water body is polluted and the like.
Example 2
The operation sites of this embodiment are: 2-mouth cultivation pond of Shandong Jining Weishan farm
In the practical use case, the water-regulating disease-preventing bio-base comprises the following components in percentage by mass: 72 percent of fermented soybean meal, 8 percent of cane sugar molasses, 14 percent of wheat bran, 0.5 percent of lactobacillus plantarum, 3 percent of bacillus coagulans, 1.5 percent of beer yeast, 0.4 percent of monopotassium phosphate, 0.4 percent of calcium dihydrogen phosphate, 0.1 percent of magnesium sulfate and 0.1 percent of sodium chloride.
In the practical use case, the application of the water diversion disease prevention bio-base in the grass carp fry rough pond is as follows:
sequentially adding water into a 3.5 mu cultivation pond to be increased to more than 2 meters, adding quicklime, disinfectant, calcium chloride and magnesium sulfate to keep the pH value of the pond to be 7.8-8.2, keeping the total alkalinity to be 150 plus materials at 300mg/L, keeping the calcium hardness to be 100 plus materials at 200mg/L, keeping the ammonia nitrogen concentration to be below 0.2mg/L and the nitrite concentration to be below 0.02mg/L, adjusting the water quality index for the second day, respectively adding chlorella culture solution and water-regulating disease-preventing bio-base into the cultivation water body, and adjusting the water volume to be 5 × 10 according to the water body volume-5Splashing the water in the whole pool (namely the inoculation amount of the floccule seeds in each cubic meter of the aquaculture water is 20 kilograms), observing the change of the water color from light green to thick after every other day according to the volume of the water body of 1 × 10-5Adding the bio-base of the embodiment into a water body, continuously monitoring index change for 5 days every day until the transparency is about 0.3m-0.5m,the ammonia nitrogen concentration is below 0.2 mg/L; when the nitrite concentration is below 0.02mg/L, 20 thousands of grass carp fries with the specification of 1cm (namely 40 fries are put in each cubic water body), and the product is splashed in the whole pool every 5 days, so that the transparency is about 0.3 m.
After the grass carp is cultured for 14 weeks, the grass carp fingerlings grow well, the body length is about 10cm, the bait coefficient is 1.5, the survival rate is 92%, the specifications are tidy, the body color and the body shape are normal, the pH value of a water body is stable, and the contents of ammonia nitrogen, nitrite and nitrate do not reach the standard exceeding level. The whole process has no large-scale epidemic disease, blue-green algae breeding and parasites, and solves the problems of the prior art that the bait coefficient is 1.7, the survival rate is 75-85%, the water body is turbid for a long time, and the like.
Example 3
The operation sites of this embodiment are: 4-mouth cultivation pond of lake-building cultivation farm in Jiangsu salt city
In the practical use case, the water-regulating disease-preventing bio-base comprises the following components in percentage by mass: 75% of fermented soybean meal, 7% of cane sugar molasses, 12% of wheat bran, 0.5% of lactobacillus plantarum, 3% of bacillus coagulans, 1.5% of saccharomyces cerevisiae, 0.4% of monopotassium phosphate, 0.4% of calcium dihydrogen phosphate, 0.1% of magnesium sulfate and 0.1% of sodium chloride.
In this example, the application of the water diversion disease prevention bio-base in the crudely pond of the crucian fingerlings:
sequentially adding water into a 3.5 mu cultivation pond to a depth of more than 2 meters, adding quicklime, disinfectant, calcium chloride and magnesium sulfate to keep the pH value of the pond at 7.8-8.0, keeping the total alkalinity at 120-200mg/L, keeping the calcium hardness at 70-100mg/L, keeping the ammonia nitrogen concentration below 0.2mg/L and the nitrite concentration below 0.02mg/L, adjusting the water quality index for the second day, respectively adding the chlorella culture solution and the water-adjusting disease-preventing bio-base into the cultivation water body, and adjusting the water volume to 5 × 10 according to the water volume-5Splashing the water in the whole pond (namely the inoculation amount of floccule seeds in each cubic meter of aquaculture water is 20 kilograms), observing that the water color is changed from light green to thick every other day according to 1 × 10 of the volume of the water body-5Adding the bio-base of the embodiment into a water body, continuously monitoring index change every day for 5 days until the transparency is about 0.3m-0.5m and the ammonia nitrogen concentration is below 0.2 mg/L; nitrite concentration below 0.02mg/L15 thousands of crucian fries (namely 30 fries are put in each cubic water body) with the specification of 0.8cm are put in the pond, and the product is sprinkled in the whole pond every 5 days to ensure that the transparency is about 0.3 m.
After 13 weeks of culture, the crucian fingerlings have the length of about 8cm, the feed coefficient is 1.3, the survival rate is 93%, the body color is bright, the body shape is normal, the pH value of the water body is stable, and the contents of ammonia nitrogen, nitrite and nitrate are normal. The whole process has no large-scale epidemic disease, and no blue-green algae breeding and parasite. After the bait is used, the problems of high bait coefficient, low survival rate, turbid water body and the like in the past are solved.
Example 4
The operation sites of this embodiment are: 2-mouth cultivation pond of Guangdong Shunde apricot jar farm
In the practical use case, the water-regulating disease-preventing bio-base comprises the following components in percentage by mass: 75% of fermented soybean meal, 7% of cane sugar molasses, 12% of wheat bran, 0.5% of lactobacillus plantarum, 3% of bacillus coagulans, 1.5% of saccharomyces cerevisiae, 0.4% of monopotassium phosphate, 0.4% of calcium dihydrogen phosphate, 0.1% of magnesium sulfate and 0.1% of sodium chloride.
In this example, the application of the water diversion disease prevention bio-base in the macro-coarse pond of micropterus salmoides fingerlings:
sequentially adding water into a 3 mu cultivation pond until the water depth is more than 2.5 m, adding quicklime, disinfectant, calcium chloride and magnesium sulfate to keep the pH value of the pond at 7.8-8.0, keeping the total alkalinity at 120-200mg/L, keeping the calcium hardness at 70-100mg/L, keeping the ammonia nitrogen concentration below 0.2mg/L and the nitrite concentration below 0.02mg/L, adjusting the water quality index for the second day, respectively adding the chlorella culture solution and the water-adjusting disease-preventing bio-base into the cultivation water body, and adjusting the water volume to 5 × 10 according to the water volume-5Splashing the water in the whole pond (namely the inoculation amount of floccule seeds in each cubic meter of aquaculture water is 20 kilograms), observing that the water color is changed from light green to thick every other day according to 1 × 10 of the volume of the water body-5Adding the bio-base of the embodiment into a water body, continuously monitoring index change every day for 5 days until the transparency is about 0.3m-0.5m and the ammonia nitrogen concentration is below 0.2 mg/L; when the nitrite concentration is below 0.02mg/L, 20 thousands of micropterus salmoides fry (namely 40 fries per cubic water body) with the specification of 0.8cm are put in the pond, and the product is splashed into the whole pond every 5 daysSprinkling to make the transparency about 0.3 m.
After one month of culture, the length of the micropterus salmoides is about 2cm, the feed coefficient is 1.3, the survival rate is 93%, the body color is bright, the body shape is normal, the pH value of the water body is stable, and the contents of ammonia nitrogen, nitrite and nitrate are normal.
Various corresponding changes and modifications can be made by those skilled in the art according to the above technical solutions and concepts, and all such changes and modifications should be included in the scope of the present invention as claimed.

Claims (3)

1. A water-regulating disease-preventing bio-base for cultivating seedlings of freshwater aquaculture animals is characterized in that the water-regulating disease-preventing bio-base is composed of fermented soybean meal, cane sugar molasses, wheat bran, lactic acid bacteria, saccharomycetes, monopotassium phosphate, monocalcium phosphate, magnesium sulfate and sodium chloride; wherein the lactobacillus is obtained by mixing Lactobacillus plantarum and Bacillus coagulans.
2. The water-regulating disease-preventing bio-base for cultivating the seedlings of the freshwater aquaculture animals according to claim 1, which is characterized in that the water-regulating disease-preventing bio-base comprises the following components in percentage by mass: 60 to 80 percent of fermented soybean meal, 5 to 15 percent of cane sugar molasses, 7 to 20 percent of wheat bran, 0.25 to 1 percent of lactobacillus plantarum, 1 to 5 percent of bacillus coagulans, 0.5 to 2 percent of saccharomyces cerevisiae, 0.15 to 0.5 percent of potassium dihydrogen phosphate, 0.15 to 0.5 percent of calcium dihydrogen phosphate, 0.1 to 0.3 percent of magnesium sulfate and 0.1 to 0.3 percent of sodium chloride.
3. The water-regulating disease-preventing bio-based for cultivating the seedlings of the freshwater aquaculture animals according to claim 2, wherein the water-regulating disease-preventing bio-based comprises the following components in percentage by mass: 72 percent of fermented soybean meal, 8 percent of cane sugar molasses, 14 percent of wheat bran, 0.5 percent of lactobacillus plantarum, 3 percent of bacillus coagulans, 1.5 percent of beer yeast, 0.4 percent of monopotassium phosphate, 0.4 percent of calcium dihydrogen phosphate, 0.1 percent of magnesium sulfate and 0.1 percent of sodium chloride.
CN202010138984.5A 2020-03-03 2020-03-03 Water-regulating disease-preventing bio-base for breeding freshwater breeding animal seedlings Pending CN111316941A (en)

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Publication number Priority date Publication date Assignee Title
CN101078003A (en) * 2006-05-23 2007-11-28 上海创博生态工程有限公司 Preparation for comprehensive treatment of cultivation water body by using multiple bacterial and manufacturing method thereof
CN107969357A (en) * 2017-11-08 2018-05-01 广州智龙生物科技有限公司 A kind of high-efficient method for cultivating of fish fry
CN108504605A (en) * 2018-04-19 2018-09-07 广州普麟生物制品有限公司 A kind of biological flocculation culture medium
CN109971682A (en) * 2019-04-01 2019-07-05 武汉丰甜生物科技有限公司 A kind of EM bacterium solution for aquaculture and preparation method thereof and application

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101078003A (en) * 2006-05-23 2007-11-28 上海创博生态工程有限公司 Preparation for comprehensive treatment of cultivation water body by using multiple bacterial and manufacturing method thereof
CN107969357A (en) * 2017-11-08 2018-05-01 广州智龙生物科技有限公司 A kind of high-efficient method for cultivating of fish fry
CN108504605A (en) * 2018-04-19 2018-09-07 广州普麟生物制品有限公司 A kind of biological flocculation culture medium
CN109971682A (en) * 2019-04-01 2019-07-05 武汉丰甜生物科技有限公司 A kind of EM bacterium solution for aquaculture and preparation method thereof and application

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