CN108504605A - A kind of biological flocculation culture medium - Google Patents
A kind of biological flocculation culture medium Download PDFInfo
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- CN108504605A CN108504605A CN201810353947.9A CN201810353947A CN108504605A CN 108504605 A CN108504605 A CN 108504605A CN 201810353947 A CN201810353947 A CN 201810353947A CN 108504605 A CN108504605 A CN 108504605A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
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Abstract
The present invention relates to a kind of biological flocculation culture mediums, are made of soybean honey, Sugar cane molasses, starch, wheat bran, lactic acid bacteria, potassium dihydrogen phosphate and calcium dihydrogen phosphate.Under suitable total alkalinity, stiffness conditions, the profitable strains such as amonifying bacteria, nitrite bacteria, nitrobacteria and denitrifying bacteria can be cultivated in water body using the biological flocculation culture medium of the present invention, and form stable denitrogenation-type biological flocculation, the floc sedimentation has " denitrogenation " ability of stability and high efficiency, assimilation and dissimilation are had both, so that waste nitrogen in water is discharged in the form of nitrogen, clean water body, systems stabilisation, whole process does not use dissipation drug, really realizes green cultivation, no pollution discharge.Periodically it can prevent and handle the cultivation problems such as bad water body water colour caused by carbon nitrogen is unbalance, ammonia nitrogen, nitrite are exceeded for a long time, pH value is high or variation is violent, blue-green alge growth, parasite is spread unchecked, water body is muddy, seriously polluted for a long time, disease is multiple using the biological flocculation culture medium.
Description
Technical field
The invention belongs to technical field of aquaculture, and in particular to a kind of biological flocculation culture medium.
Background technology
Biological flocculation technology (BFT) is the activated sludge technology used for reference in municipal sewage treatment, by artificially to cultivation water
Organic carbonizable substance (such as molasses, glucose) is added in body, adjusts the carbon-nitrogen ratio (C/N) in water body, and it is thin to improve heterotrophism in water body
The nitrogenous compounds such as ammonia nitrogen in water body are converted to mycoprotein, formation can using microbial assimilation inorganic nitrogen by the quantity of bacterium
The biological floes directly ingested by filter-feeding cultivation object, can solve detritus and feed retention problems in breeding water body, real
The recycling of existing bait plays and purifies water, reduces quantity of exchanged water, saves feed, improves cultivation object survival rate and increase yield
The effects that a technology.
According to existing report it is found that the structure composition of biological flocculation is considerably complicated, formation mechenism is also not fully understood,
Academia is it is believed that the formation of floc sedimentation is mainly caused by algae or bacterium are flocculated.It is more research report with
Glycerine, rice bran, tapioca starch, sucrose, starch, molasses and glucose etc. are used as carbon source, can form biological flocculation very well, and
The growth for promoting cultivated animals, purifies water.The high carbohydrate of phosphorus content or input albumen are added in cultivating pool
The lower feed of matter content can effectively promote the carbon-nitrogen ratio in water, and when carbon-nitrogen ratio is more than 10 or more, pond Central Plains is with autotrophy
Cultivating system based on bacterium translates into the biological flocculation system based on heterotrophicy bacteria.In fact carbon-nitrogen ratio in pond waters
It is general relatively low, it is therefore desirable to Additional carbon sources to be added into water body, to improve the ratio of carbon nitrogen.Carbon-nitrogen ratio is biological flocculation technology
Core, many researchs report the case where carbon-nitrogen ratio is 20 and 10, and in general, heterotrophicy bacteria needs from absorbing extraneous nitrogen member
Element assimilates into own cells ingredient, this depends on the carbon-nitrogen ratio of heterotrophicy bacteria itself, and the carbon-nitrogen ratio of heterotrophicy bacteria itself is about
4, that is, when a nitrogen of heterotrophicy bacteria assimilation, need to consume about 4 parts of organic carbons.Heterotrophicy bacteria is usual to the assimilability of organic carbon
It is 40%, that is, needs 10 parts of organic carbons, is 10 here it is the carbon-nitrogen ratio carried, it is assumed that the organic carbon source of offer contains 50%
Carbon, at this time will be about consumption and utilize 20 parts of organic carbon sources, that is, the carbon-nitrogen ratio that is carried is 20.Carbon-nitrogen ratio is current biology
Floc sedimentation technical field research hotspot, the feed carbon-nitrogen ratio fed in intensive culture are generally 10 or so, but heterotrophicy bacteria
A nitrogen of assimilation needs 20 parts of carbon.If the feed carbon-nitrogen ratio of input is too low in cultivation, heterotrophicy bacteria grows needed nutrient
Matter is limited by carbon, is eventually led to heterotrophicy bacteria and is unable to mass propagation, at this moment needs to increase water body by adding Additional carbon sources
In carbon, promote the mass propagation of heterotrophicy bacteria, cultivating system made to be changed into heterotroph by autotrophic type.Heterotrophicy bacteria group utilizes
The organic nitrogen of ammonia nitrogen and residue in water synthesizes own cells ingredient (microprotein), can be used as carp, Tilapia mossambica, seawater shrimps
Class, fresh water shrimp daily bait and be utilized.
Therefore, exploitation is a kind of having stable and efficient " denitrogenation " ability, and having both assimilation and dissimilation (makes waste nitrogen in water
Water body is left in the form of nitrogen), clean water body, systems stabilisation biological flocculation culture medium have great meaning.
Invention content
The object of the present invention is to provide a kind of biological flocculation culture mediums periodically to make under suitable total alkalinity, stiffness conditions
With the present invention biological flocculation culture medium can gradually be cultivated in water body amonifying bacteria, nitrite bacteria, nitrobacteria and
The profitable strains such as denitrifying bacteria, and stable denitrogenation-type biological flocculation is formed, which has stable and efficient " denitrogenation "
Ability has both assimilation and dissimilation (waste nitrogen in water is made to leave water body in the form of nitrogen), clean water body, systems stabilisation, cultivation
Whole process does not use dissipation drug, to really realize green cultivation, no pollution discharge.It periodically can using the biological flocculation culture medium
Effectively prevent and processing water body water colour caused by carbon nitrogen is unbalance is bad, ammonia nitrogen, nitrite is exceeded for a long time, pH value residence is high not
The cultivation such as lower or variation is acutely, blue-green alge grows, parasite is spread unchecked, water body is muddy, seriously polluted for a long time, disease is multiple are difficult
Topic.
The technical scheme is that：
A kind of biological flocculation culture medium, by soy molasses, Sugar cane molasses, Tapioca starch, wheat bran skin, lactic acid bacteria, potassium dihydrogen phosphate
It is formed with calcium dihydrogen phosphate.
Preferably, the mass percentage of each component is in above-mentioned biological flocculation culture medium：
Soy molasses and Sugar cane molasses 70%-90%, Tapioca starch and wheat bran skin 8%-20%, lactic acid bacteria 1%-5%, di(2-ethylhexyl)phosphate
Hydrogen potassium and calcium dihydrogen phosphate 1%-5%.
It is highly preferred that the mass percentage of each component is in above-mentioned biological flocculation culture medium：
Soy molasses and Sugar cane molasses 80%, Tapioca starch and wheat bran skin 15%, lactic acid bacteria 3%, potassium dihydrogen phosphate and di(2-ethylhexyl)phosphate
Hydrogen calcium 2%.
Beneficial effects of the present invention are as follows：
(1) growth of denitrogenation-type beneficial microbe population, selectivity is stimulated to cultivate pond microorganism.
(2) stable and efficient denitrogenation-type biological flocculation is cultivated, water body self-purification ability is improved, increases productivity.
(3) waste nitrogen pollution and the conversion in breeding process, effectively prevention and processing ammonia nitrogen, nitrite, nitrate are controlled
Excessive problem.
(4) stablize pH, stablize water environment, stablize the ecosystem.
Specific implementation mode
With reference to embodiment, technical scheme of the present invention is described in further detail, but do not constituted pair
Any restrictions of the present invention.
Biological denitrifier is bought from Guangzhou Pu Lin biological products Co., Ltd in following embodiment；Following embodiment is used
Floc sedimentation kind be with reference to " a kind of denitrogenation-type biological flocculation kind acclimatization culture method " test pond voluntarily prepare.
Embodiment 1
The mass percentage of the present embodiment biological flocculation culture medium each component is：Soybean honey and Sugar cane molasses 70%,
Starch and wheat bran 20%, lactic acid bacteria 5%, potassium dihydrogen phosphate and calcium dihydrogen phosphate 5%.
Application of the present embodiment biological flocculation culture medium in Tilapia mossambica breeding water body：
Quick lime, disinfectant, calcium chloride, magnesium sulfate, sodium bicarbonate are put into aquaculture pond successively, its pH is made to be maintained at 8.0-
8.2；Total alkalinity is maintained at 120-200ppm；Calcium ion concentration is maintained at 72-120ppm；Magnesium ion concentration is maintained at 40-
100ppm；Ammonia nitrogen concentration is in 0.2ppm or less；Nitrite concentration is in 0.02ppm or less.Mix up water quality index second day, respectively
The biological flocculation culture medium of biological denitrifier and the present embodiment is added into breeding pool, changes full pool spilling head after water respectively, makes life
Object denitrifier and biological flocculation culture medium concentration are 50ppm；Then floc sedimentation kind is accessed, every cubic metre of breeding water body floc sedimentation kind connects
Kind amount is 20 kilograms；Then it is added the biological flocculation culture medium 10ppm of the present embodiment into breeding water body daily, continuous 7 days, often
Its monitoring index changes, and waits for that floc sedimentation amount is 2-4ml/L, ammonia nitrogen concentration is in 0.2ppm or less；Nitrite concentration 0.02ppm with
When lower, it is put into the Rofe fry that specification is 3cm, every cube of water body puts 80 tails in a suitable place to breed, feeds food daily and by the present embodiment biology
Full pool spilling head after floc sedimentation culture medium water makes its a concentration of 10ppm.
After cultivating 12 weeks, Tilapia mossambica growing way is preferable, does not find that disease, water pH value are stablized, ammonia nitrogen, nitrite and nitric acid
Salt content does not reach exceeded level.The problems such as blue-green alge grows, parasite is spread unchecked, water body is muddy for a long time, pollution, which has, to be changed
It is kind.
Embodiment 2
The mass percentage of the present embodiment biological flocculation culture medium each component is：Soybean honey and Sugar cane molasses 90%,
Starch and wheat bran 8%, lactic acid bacteria 1%, potassium dihydrogen phosphate and calcium dihydrogen phosphate 1%.
Quick lime, disinfectant, calcium chloride, magnesium sulfate, sodium bicarbonate are put into aquaculture pond successively, its pH is made to be maintained at 8.0-
8.2；Total alkalinity is maintained at 120-200ppm；Calcium ion concentration is maintained at 72-120ppm；Magnesium ion concentration is maintained at 40-
100ppm；Ammonia nitrogen concentration is in 0.2ppm or less；Nitrite concentration is in 0.02ppm or less.Mix up water quality index second day, respectively
The biological flocculation culture medium of biological denitrifier and the present embodiment is added into breeding pool, changes full pool spilling head after water respectively, makes life
Object denitrifier and biological flocculation culture medium concentration are 50ppm；Then floc sedimentation kind is accessed, every cubic metre of breeding water body floc sedimentation kind connects
Kind amount is 30 kilograms；Then it is added the present embodiment biological flocculation culture medium 15ppm into breeding water body daily, continuous 7 days, daily
Monitoring index changes, and waits for that floc sedimentation amount is 2-4ml/L, ammonia nitrogen concentration is in 0.2ppm or less；Nitrite concentration is in 0.02ppm or less
When, it is put into the Rofe fry that specification is 3cm, every cube of water body puts 90 tails in a suitable place to breed, feeds food daily and the present embodiment biology is wadded a quilt with cotton
Full pool spilling head after group's culture medium water, the mass percent of the input amount and same day food input amount of biological flocculation culture medium are
10%.
After cultivating 12 weeks, Tilapia mossambica growing way is preferable, does not find that disease, water pH value are stablized, ammonia nitrogen, nitrite and nitric acid
Salt content does not reach exceeded level.The problems such as blue-green alge grows, parasite is spread unchecked, water body is muddy for a long time, pollution, which has, to be changed
It is kind.
Embodiment 3
The mass percentage of the present embodiment biological flocculation culture medium each component is：Soybean honey and Sugar cane molasses 80%,
Starch and wheat bran 15%, lactic acid bacteria 3%, potassium dihydrogen phosphate and calcium dihydrogen phosphate 2%.
Quick lime, disinfectant, calcium chloride, magnesium sulfate, sodium bicarbonate are put into aquaculture pond successively, its pH is made to be maintained at 8.0-
8.2；Total alkalinity is maintained at 120-200ppm；Calcium ion concentration is maintained at 72-120ppm；Magnesium ion concentration is maintained at 40-
100ppm；Ammonia nitrogen concentration is in 0.2ppm or less；Nitrite concentration is in 0.02ppm or less.Mix up water quality index second day, respectively
The biological flocculation culture medium of biological denitrifier and the present embodiment is added into breeding pool, changes full pool spilling head after water respectively, makes life
Object denitrifier and biological flocculation culture medium concentration are 50ppm；Then floc sedimentation kind is accessed, every cubic metre of breeding water body floc sedimentation kind connects
Kind amount is 30 kilograms；Then it is added biological flocculation culture medium 20ppm of the present invention into breeding water body daily, continuous 7 days, every heavenly prison or jail
Index variation is surveyed, waits for that floc sedimentation amount is 2-4ml/L, ammonia nitrogen concentration is in 0.2ppm or less；Nitrite concentration is in 0.02ppm or less
When, it is put into the Rofe fry that specification is 4cm, every cube of water body puts 100 tails in a suitable place to breed, feeds food daily and by biological flocculation of the present invention
The mass percent of full pool spilling head after culture medium water, the input amount and same day food input amount of biological flocculation culture medium is
15%.
After cultivating 12 weeks, Tilapia mossambica growing way is preferable, does not find that disease, water pH value are stablized, ammonia nitrogen, nitrite and nitric acid
Salt content does not reach exceeded level.The problems such as blue-green alge grows, parasite is spread unchecked, water body is muddy for a long time, pollution has bright
It is aobvious to improve.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, it is other it is any without departing from the spirit and principles of the present invention made by changes, modifications, substitutions, combinations, simplifications,
Equivalent substitute mode is should be, is included within the scope of the present invention.
Claims (3)
1. a kind of biological flocculation culture medium, it is characterised in that：By soy molasses, Sugar cane molasses, Tapioca starch, wheat bran skin, lactic acid bacteria, phosphorus
Acid dihydride potassium and calcium dihydrogen phosphate composition.
2. a kind of biological flocculation culture medium according to claim 1, which is characterized in that each in the biological flocculation culture medium
The mass percentage of component is：
Soy molasses and Sugar cane molasses 70%-90%, Tapioca starch and wheat bran skin 8%-20%, lactic acid bacteria 1%-5%, potassium dihydrogen phosphate
With calcium dihydrogen phosphate 1%-5%.
3. a kind of biological flocculation culture medium according to claim 1, which is characterized in that each in the biological flocculation culture medium
The mass percentage of component is：
Soy molasses and Sugar cane molasses 80%, Tapioca starch and wheat bran skin 15%, lactic acid bacteria 3%, potassium dihydrogen phosphate and calcium dihydrogen phosphate
2%.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109607820A (en) * | 2018-12-19 | 2019-04-12 | 深圳合民生物科技有限公司 | A kind of water remediation method |
| CN109913383A (en) * | 2018-10-25 | 2019-06-21 | 中国环境科学研究院 | Nitrogen cycle microbial inoculum and the preparation method and application thereof for low concentration nitric wastewater |
| CN111316941A (en) * | 2020-03-03 | 2020-06-23 | 中国水产科学研究院珠江水产研究所 | Water-regulating disease-preventing bio-base for breeding freshwater breeding animal seedlings |
| CN111467412A (en) * | 2020-04-10 | 2020-07-31 | 广东汇海农牧科技集团有限公司 | Dace pest control preparation and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109913383A (en) * | 2018-10-25 | 2019-06-21 | 中国环境科学研究院 | Nitrogen cycle microbial inoculum and the preparation method and application thereof for low concentration nitric wastewater |
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| CN111316941A (en) * | 2020-03-03 | 2020-06-23 | 中国水产科学研究院珠江水产研究所 | Water-regulating disease-preventing bio-base for breeding freshwater breeding animal seedlings |
| CN111467412A (en) * | 2020-04-10 | 2020-07-31 | 广东汇海农牧科技集团有限公司 | Dace pest control preparation and application thereof |
| CN111467412B (en) * | 2020-04-10 | 2021-07-23 | 广东汇海农牧科技集团有限公司 | Dace pest control preparation and application thereof |
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Application publication date: 20180907 |