CN111308069B - 一种测试噻虫胺的时间分辨荧光免疫层析试纸卡 - Google Patents

一种测试噻虫胺的时间分辨荧光免疫层析试纸卡 Download PDF

Info

Publication number
CN111308069B
CN111308069B CN202010158854.8A CN202010158854A CN111308069B CN 111308069 B CN111308069 B CN 111308069B CN 202010158854 A CN202010158854 A CN 202010158854A CN 111308069 B CN111308069 B CN 111308069B
Authority
CN
China
Prior art keywords
clamping part
film
pad
clothianidin
microspheres
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202010158854.8A
Other languages
English (en)
Other versions
CN111308069A (zh
Inventor
王秀国
张晓�
郭庆
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tobacco Research Institute of CAAS
Original Assignee
Tobacco Research Institute of CAAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tobacco Research Institute of CAAS filed Critical Tobacco Research Institute of CAAS
Priority to CN202010158854.8A priority Critical patent/CN111308069B/zh
Priority to NZ765284A priority patent/NZ765284A/en
Publication of CN111308069A publication Critical patent/CN111308069A/zh
Priority to US16/924,890 priority patent/US11619584B2/en
Application granted granted Critical
Publication of CN111308069B publication Critical patent/CN111308069B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G01N33/54387—Immunochromatographic test strips
    • G01N33/54388—Immunochromatographic test strips based on lateral flow
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N21/643—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" non-biological material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84—Systems specially adapted for particular applications
    • G01N21/8483—Investigating reagent band
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N2021/7769—Measurement method of reaction-produced change in sensor
    • G01N2021/7786—Fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2430/00—Assays, e.g. immunoassays or enzyme assays, involving synthetic organic compounds as analytes
    • G01N2430/10—Insecticides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

本发明提供一种测试噻虫胺的时间分辨荧光免疫层析试纸卡，具体涉及噻虫胺检测领域；结合垫上包括检测微球和质控微球，检测微球为表面包被有噻虫胺单抗的荧光微球，质控微球为表面包被有兔抗标记蛋白的荧光微球；NC膜上设有检测线和质控线，检测线和质控线的长度均与NC膜的宽度相同，检测线上包被有噻虫胺抗原，质控线上包被有抗兔抗体；样品垫一端设有第一卡持部；结合垫两端分别设有第二卡持部和第三卡持部；NC膜一端设有第四卡持部，NC膜另一端与吸收垫衔接；且第一卡持部、第二卡持部、第三卡持部和第四卡持部均为半弧形结构；第一卡持部与第二卡持部相互贴合，第三卡持部与第四卡持部相互贴合；本发明样品扩散速度快，可大大提高检测灵敏度和检测效率。

Description

一种测试噻虫胺的时间分辨荧光免疫层析试纸卡
技术领域
本发明属于噻虫胺检测领域，具体涉及一种测试噻虫胺的时间分辨荧光免疫层析试纸卡。
背景技术
噻虫胺是高效安全的新型杀虫剂，具有触杀、胃毒和内吸活性，噻虫胺主要用于农作物上防治半翅目、鞘翅目、双翅目和某些鳞翅目类害虫，具有优异的内吸和渗透作用，比传统烟碱类杀虫剂优异；随着人们对健康问题的逐渐重视，农药残留的检测也日益成为热点，传统的噻虫胺多采用色谱法进行检测，但此类检测方法，虽然检测准确，但由于前处理复杂，导致检测成本高、时间长，不能满足大量快速检测的需求。
鉴于现有技术的不足，现需要一种测试噻虫胺的时间分辨荧光免疫层析试纸卡，在保证检测灵敏度的前提下，提高样品的检测效率。
发明内容
本发明的目的是提供一种测试噻虫胺的时间分辨荧光免疫层析试纸卡，提高样品的吸收速度和扩散速度，提高检测灵敏度和检测效率。
本发明提供了如下的技术方案：
一种测试噻虫胺的时间分辨荧光免疫层析试纸卡，包括底板，所述底板上依次设有样品垫、结合垫、NC膜和吸收垫；所述结合垫上包括检测微球和质控微球，所述检测微球为表面包被有噻虫胺单抗的荧光微球，所述质控微球为表面包被有兔抗标记蛋白的荧光微球；所述NC膜上设有检测线和质控线，所述检测线和所述质控线的长度均与所述NC膜的宽度相同，所述检测线上包被有噻虫胺抗原，所述质控线上包被有抗兔抗体；所述样品垫一端设有第一卡持部；所述结合垫两端分别设有第二卡持部和第三卡持部；所述NC膜一端设有第四卡持部，所述NC膜另一端与所述吸收垫衔接；且所述第一卡持部、第二卡持部、第三卡持部和第四卡持部均为半弧形结构；所述第一卡持部与所述第二卡持部相互贴合，所述第三卡持部与所述第四卡持部相互贴合；弧形的结构可第一卡持部、第二卡持部、第三卡持部和第四卡持部之间更好，更便捷准确的衔接；第一卡持部与第二卡持部相互贴合，第三卡持部与第四卡持部相互贴合；通过半弧状的卡持部提高样品垫与结合垫之间的结合面积，以及结合垫和NC膜的结合面积，可大大提高样品的扩散速度，进而可有效提高检测的效率和灵敏度，通过半弧状卡持部的相互卡合，可提高试纸的结构稳定性，确保产品质量的可靠性；
优选的，所述样品垫另一端上侧面设有若干凹槽状结构，参见附图1，如此可大大提高样品的扩散接触面积，加快样品的扩散速度，进而提高吸收的速度，利于提高检测效率和灵敏度；
优选的，所述第一卡持部与所述样品垫；所述第二卡持部、第三卡持部与结合垫；第四卡持部与所述NC膜均为一体成型结构，一体成型结构牢靠，且结合稳定；
优选的，所述底板上设有半弧形的第一支撑部和第二支撑部；所述第一支撑部位于第二卡持部下侧；第二支撑部位于第三卡持部下侧，通过支撑部的支撑作用，可提高卡持部的稳定性；
优选的，所述NC膜与所述吸收垫的衔接处呈波浪形，即所述NC膜的端面上呈凹凸相间的结构；所述吸收垫的端面上呈凹凸相间的结构；所述NC膜与所述吸收垫相互嵌入卡合；如此结构可大大提高样品的扩散速度，进而可有效提高检测的效率和灵敏度，通过半弧状卡持部的相互卡合，可提高试纸的结构稳定性，确保产品质量的可靠性；
优选的，所述吸收垫中部设置隆起的缓冲部，所述缓冲部下方位于所述底板上设有第三支撑部，第三支撑部发挥支撑作用提高装置的结构稳定性；
优选的，所述第一卡持部与所述第二卡持部、所述第三卡持部和所述第四卡持部的贴合处通过凹凸相间的结构相互卡合，除可确保卡合的稳定，还能提高接触面积，利于加快扩散速度，进而利于提高检测效率；
优选的，所述第二卡持部的上侧面设有凸起，所述凸起的宽度小于所述第二卡持部宽度，如此可确保卡合的稳定和增大接触面积；所述第一卡持部下侧面设有条形的凹槽；所述凸起嵌入所述凹槽内，可提高样品的接触面积，进而提高吸收的速度，可大大提高样品的扩散速度。
本发明的有益效果：
本发明通过提高样品垫与结合垫之间的结合面积，以及结合垫和NC膜的结合面积，可大大提高样品的扩散速度，进而可有效提高检测的效率和灵敏度，通过卡持部的结构可提高试纸的结构稳定性，确保产品质量的可靠性。
附图说明
附图用来提供对本发明的进一步理解，并且构成说明书的一部分，与本发明的实施例一起用于解释本发明，并不构成对本发明的限制。在附图中：
图1是本发明主视图；
图2是本发明分解结构图；
图中标记为：1、底板；2、样品垫；3、结合垫；4、NC膜；5、吸收垫；6、第一卡持部；7、第二卡持部；8、第三卡持部；9、第四卡持部；10、检测线；11、质控线；12、缓冲部；13、第一支撑部；14、第二支撑部；15、第三支撑部。
具体实施方式
如图1和图2，一种测试噻虫胺的时间分辨荧光免疫层析试纸卡，包括底板1，底板1上依次设有样品垫2、结合垫3、NC膜4和吸收垫5；结合垫3上包括检测微球和质控微球，检测微球为表面包被有噻虫胺单抗的荧光微球，质控微球为表面包被有兔抗标记蛋白的荧光微球；NC膜4上设有检测线10和质控线11，检测线10和质控线11的长度均与NC膜4的宽度相同，检测线10上包被有噻虫胺抗原，质控线11上包被有抗兔抗体；样品垫2一端设有用于卡合的第一卡持部6；结合垫3两端分别设有用于卡合的第二卡持部7和第三卡持部8；NC膜4一端设有用于卡合的第四卡持部9，NC膜4另一端与吸收垫5衔接；且第一卡持部6、第二卡持部7、第三卡持部8和第四卡持部9均为半弧形结构；弧形的结构可保障第一卡持部6、第二卡持部7、第三卡持部8和第四卡持部9之间更好，更便捷准确的衔接；
其中，第一卡持部6与第二卡持部7相互贴合，第三卡持部8与第四卡持部9相互贴合；通过半弧状的卡持部提高样品垫2与结合垫3之间的结合面积，以及结合垫3和NC膜4的结合面积，可大大提高样品的扩散速度，进而可有效提高检测的效率和灵敏度，通过半弧状卡持部的相互卡合，可提高试纸的结构稳定性，确保产品质量的可靠性；
其中，样品垫2另一端上侧面设有若干凹槽状结构，参见附图1，如此可大大提高样品的扩散接触面积，加快样品的扩散速度，进而提高吸收的速度，利于提高检测效率和灵敏度；第一卡持部6与样品垫2；第二卡持部7、第三卡持部8与结合垫3；第四卡持部9与NC膜4均为一体成型结构，如此可提高装置的韧性和使用寿命；底板1上设有半弧形的第一支撑部13、第二支撑部14；第一支撑部13位于第二卡持部7下侧；第二支撑部14位于第三卡持部8下侧；通过支撑部可提高支撑稳固度，利于提高装置的可靠性；NC膜4与吸收垫5的衔接处呈波浪形，即NC膜4的端面上呈凹凸相间的结构；吸收垫5的端面上呈凹凸相间的结构；NC膜4与吸收垫5相互嵌入卡合；吸收垫5中部设置隆起的缓冲部12，缓冲部12下方位于底板1上设有第三支撑部15，第三支撑部15可提高结构的稳定性。第一卡持部6与第二卡持部7、第三卡持部8和第四卡持部9的贴合处通过凹凸相间的结构相互卡合，该结构可提高样品的吸收接触面积，进而提高吸收的速度和扩散速度；第二卡持部7的上侧面设有凸起，凸起的宽度小于第二卡持部7宽度，如此确保整个卡持部结构稳定。第一卡持部6下侧面设有条形的凹槽；凸起嵌入凹槽内；可提高样品的接触面积，进而提高吸收的速度，可大大提高样品的扩散速度；
本发明的优点：通过半弧状的卡持部提高样品垫2与结合垫3之间的结合面积，以及结合垫3和NC膜4的结合面积，可大大提高样品的扩散速度，进而可有效提高检测的效率和灵敏度，通过半弧状卡持部的相互卡合，可提高试纸的结构稳定性，确保产品质量的可靠性。
以上仅为本发明的优选实施例而已，并不用于限制本发明，尽管参照前述实施例对本发明进行了详细的说明，对于本领域的技术人员来说，其依然可以对前述各实施例所记载的技术方案进行修改，或者对其中部分技术特征进行等同替换。凡在本发明的精神和原则之内，所作的任何修改、等同替换、改进等，均应包含在本发明的保护范围之内。

Claims (8)

1.一种测试噻虫胺的时间分辨荧光免疫层析试纸卡，其特征在于：包括底板，所述底板上依次设有样品垫、结合垫、NC膜和吸收垫；
所述结合垫上包括检测微球和质控微球，所述检测微球为表面包被有噻虫胺单抗的荧光微球，所述质控微球为表面包被有兔抗标记蛋白的荧光微球；
所述NC膜上设有检测线和质控线，所述检测线和所述质控线的长度均与所述NC膜的宽度相同，所述检测线上包被有噻虫胺抗原，所述质控线上包被有抗兔抗体；
所述样品垫一端设有第一卡持部；所述结合垫两端分别设有第二卡持部和第三卡持部；所述NC膜一端设有第四卡持部，所述NC膜另一端与所述吸收垫衔接；且所述第一卡持部、第二卡持部、第三卡持部和第四卡持部均为半弧形结构；所述第一卡持部与所述第二卡持部相互贴合，所述第三卡持部与所述第四卡持部相互贴合，所述第四卡持部在第三卡持部的上面。
2.根据权利要求1所述的一种测试噻虫胺的时间分辨荧光免疫层析试纸卡，其特征在于：所述样品垫另一端上侧面设有若干凹槽状结构。
3.根据权利要求2所述的一种测试噻虫胺的时间分辨荧光免疫层析试纸卡，其特征在于：所述第一卡持部与所述样品垫；所述第二卡持部、第三卡持部与结合垫；第四卡持部与所述NC膜均为一体成型结构。
4.根据权利要求3所述的一种测试噻虫胺的时间分辨荧光免疫层析试纸卡，其特征在于：所述底板上设有半弧形的第一支撑部和第二支撑部；所述第一支撑部位于第二卡持部下侧；第二支撑部位于第三卡持部下侧。
5.根据权利要求4所述的一种测试噻虫胺的时间分辨荧光免疫层析试纸卡，其特征在于：所述NC膜与所述吸收垫的衔接处呈波浪形，即所述NC膜的端面上呈凹凸相间的结构；所述吸收垫的端面上呈凹凸相间的结构；所述NC膜与所述吸收垫相互嵌入卡合。
6.根据权利要求5所述的一种测试噻虫胺的时间分辨荧光免疫层析试纸卡，其特征在于：所述吸收垫中部设置隆起的缓冲部，所述缓冲部下方位于所述底板上设有第三支撑部。
7.根据权利要求6所述的一种测试噻虫胺的时间分辨荧光免疫层析试纸卡，其特征在于：所述第一卡持部与所述第二卡持部、所述第三卡持部和所述第四卡持部的贴合处通过凹凸相间的结构相互卡合。
8.根据权利要求7所述的一种测试噻虫胺的时间分辨荧光免疫层析试纸卡，其特征在于：所述第二卡持部的上侧面设有凸起，所述凸起的宽度小于所述第二卡持部宽度；所述第一卡持部下侧面设有条形的凹槽；所述凸起嵌入所述凹槽内。
CN202010158854.8A 2020-03-09 2020-03-09 一种测试噻虫胺的时间分辨荧光免疫层析试纸卡 Active CN111308069B (zh)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CN202010158854.8A CN111308069B (zh) 2020-03-09 2020-03-09 一种测试噻虫胺的时间分辨荧光免疫层析试纸卡
NZ765284A NZ765284A (en) 2020-03-09 2020-06-10 Time-resolved fluorescence immunochromatographic test paper card for testing clothianidin
US16/924,890 US11619584B2 (en) 2020-03-09 2020-07-09 Time-resolved fluorescence immunochromatographic test paper card for testing clothianidin

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010158854.8A CN111308069B (zh) 2020-03-09 2020-03-09 一种测试噻虫胺的时间分辨荧光免疫层析试纸卡

Publications (2)

Publication Number Publication Date
CN111308069A CN111308069A (zh) 2020-06-19
CN111308069B true CN111308069B (zh) 2023-06-06

Family

ID=71145481

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202010158854.8A Active CN111308069B (zh) 2020-03-09 2020-03-09 一种测试噻虫胺的时间分辨荧光免疫层析试纸卡

Country Status (3)

Country Link
US (1) US11619584B2 (zh)
CN (1) CN111308069B (zh)
NZ (1) NZ765284A (zh)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113758915A (zh) * 2021-04-23 2021-12-07 威海纽普生物技术有限公司 新型侧向层析检测卡

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0316177A2 (en) * 1987-11-10 1989-05-17 Tai-Her Yang Gripping device
CA3039377A1 (en) * 2017-02-22 2018-08-22 Jacek Krzyzanowski Jaw assembly for surgical tool and biopsy sample collection member
CN108802358A (zh) * 2018-06-29 2018-11-13 云南中烟工业有限责任公司 一种时间分辨荧光微球标记的二苯甲酮类化合物快检试纸条及制备方法
CN109061158A (zh) * 2018-09-21 2018-12-21 中国烟草总公司郑州烟草研究院 一种检测啶虫脒的时间分辨荧光免疫层析试纸条及其制备方法和应用
CN109975557A (zh) * 2019-04-29 2019-07-05 厦门稀土材料研究所 Il-6/pct联合检测时间分辨检测试剂盒及方法
CN109996606A (zh) * 2016-11-23 2019-07-09 生物辐射实验室股份有限公司 侧流试验装置

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0316177A2 (en) * 1987-11-10 1989-05-17 Tai-Her Yang Gripping device
CN109996606A (zh) * 2016-11-23 2019-07-09 生物辐射实验室股份有限公司 侧流试验装置
CA3039377A1 (en) * 2017-02-22 2018-08-22 Jacek Krzyzanowski Jaw assembly for surgical tool and biopsy sample collection member
CN108802358A (zh) * 2018-06-29 2018-11-13 云南中烟工业有限责任公司 一种时间分辨荧光微球标记的二苯甲酮类化合物快检试纸条及制备方法
CN109061158A (zh) * 2018-09-21 2018-12-21 中国烟草总公司郑州烟草研究院 一种检测啶虫脒的时间分辨荧光免疫层析试纸条及其制备方法和应用
CN109975557A (zh) * 2019-04-29 2019-07-05 厦门稀土材料研究所 Il-6/pct联合检测时间分辨检测试剂盒及方法

Also Published As

Publication number Publication date
US20210278344A1 (en) 2021-09-09
NZ765284A (en) 2022-01-28
CN111308069A (zh) 2020-06-19
US11619584B2 (en) 2023-04-04

Similar Documents

Publication Publication Date Title
US6368876B1 (en) One step immunochromatographic device and method of use
US6399398B1 (en) Assay device
AU742664B2 (en) Assay devices
JP3727952B2 (ja) 自動化イムノアッセイカセット
US6472226B1 (en) Assay using porosity-reduction to inhibit migration
CN111308069B (zh) 一种测试噻虫胺的时间分辨荧光免疫层析试纸卡
US8062908B2 (en) Device for collection and assay of oral fluids
US20020155029A1 (en) Device for collection and assay of oral fluids
IT9052873U1 (it) Dispositivo per prove analitiche.
EP0491024A1 (en) Analytical test device for specific binding assays
CN109142707B (zh) 一种荧光免疫层析测量装置
EP3910333A1 (en) Sample loading bottom plate and immunochromatography detection apparatus containing sample loading bottom plate
US20220252591A1 (en) Liquid sample testing device
KR20020096070A (ko) 투명한 플라스틱 지지체를 가진 면역크로마토그래피 분석스트립, 분석 장치 및 그의 제조방법
KR20230050350A (ko) 측방 유동 기반 생물학적 샘플 수집 및 진단용 장치와 그 사용 방법
KR102616721B1 (ko) 면역 크로마토그래피 검출 장치
US11828756B2 (en) Lateral flow test arrangement suitable for detection of an analyte in saliva
CN212622625U (zh) 一种二联抗体快检试剂盒
CN201096787Y (zh) C-反应蛋白颜色颗粒诊断试纸
CN113758915A (zh) 新型侧向层析检测卡
CN220207623U (zh) 一种多功能双向层析检测试纸条
CN210136234U (zh) 免疫层析检测装置
CA2652681C (en) Sample pad system for controlling fluid movement between a sample receiving pad and a test strip
CN212364322U (zh) 一种用于n-端脑利钠肽前体测定的免疫层析试纸
CN211826080U (zh) 一种牛布氏杆菌病快速诊断检测试纸条

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant