CN111278848A - 特异性识别甲基化修饰dna碱基的tale rvd及其应用 - Google Patents

特异性识别甲基化修饰dna碱基的tale rvd及其应用 Download PDF

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CN111278848A
CN111278848A CN201780093389.0A CN201780093389A CN111278848A CN 111278848 A CN111278848 A CN 111278848A CN 201780093389 A CN201780093389 A CN 201780093389A CN 111278848 A CN111278848 A CN 111278848A
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tale
target sequence
rvd
fusion protein
gene
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CN111278848B (zh
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魏文胜
伊成器
张媛
郭生杰
朱晨旭
刘璐璐
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Edigene Beijing Biotechnology Co ltd
Peking University
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Peking University
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Abstract

本发明提供了对于5mC、5hmC和6mA具有识别偏好和不同结合特性的RVD，以及包含含有上述RVD的TALE重复结构域的分离的DNA结合多肽、融合蛋白、其编码多核苷酸、包含多核苷酸的载体以及宿主细胞。本发明还提供上述DNA结合多肽、融合蛋白在甲基化依赖的基因激活、高效基因组编辑、检测目标基因靶序列中的甲基化碱基以及靶向结合细胞中目标基因中的应用。

Description

PCT国内申请，说明书已公开。

Claims (25)

  1. PCT国内申请，权利要求书已公开。
CN201780093389.0A 2017-08-04 2017-08-04 特异性识别甲基化修饰dna碱基的tale rvd及其应用 Active CN111278848B (zh)

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