CN111265467A - An anti-allergy repairing agent prepared by mixing stem cell extract and compound plant extract, and its preparation method - Google Patents

Abstract

The invention discloses an anti-allergy repairing agent prepared by mixing a stem cell extract and a composite plant extract, which comprises a skin stem cell whole extracting solution and the composite plant extract; the skin stem cell whole extracting solution is obtained by culturing human skin stem cells for more than 3 days, crushing and centrifuging the whole culture solution mixture of the stem cells, taking supernate, and filtering the supernate by a filter membrane; the complex plant extract includes centella asiatica extract, chamomilla extract, yarrow extract, purslane extract, and malva sylvestris extract. The ingredients selected in the invention are safe, non-irritant and non-toxic, and have obvious mutual synergistic effect among various ingredients, so that the skin sensitivity problem can be solved, the damaged barrier of the sensitive skin can be effectively repaired, and the skin sensitivity can be improved by resisting oxidation, accelerating the circulation metabolism, enhancing the immunity of the damaged skin.

Description

An anti-allergy repairing agent prepared by mixing stem cell extract and compound plant extract, and its preparation method

Technical Field

The invention relates to the field of daily cosmetics, in particular to an anti-allergy repairing agent prepared by mixing a stem cell extract and a composite plant extract and a preparation method thereof.

Background

The number of people with sensitive skin is increasing due to increasing air pollution, external environmental factors such as pollen, certain foods, etc., and increasing use of cosmetics and chemical preparations (including inferior cosmetics for long-term use, cosmetics containing hormones and antibiotics, etc.). Sensitive skin generally has the following characteristics: the skin is thin and easy to be allergic, the red blood on the face is obvious, the skin is easy to be reddened and heated due to general temperature change, supercooling or overheating, and the skin is easy to be stimulated by environmental factors, seasonal changes and facial care products.

Most skin care products in the market at present are various in types, but the skin care products are different in quality, contain a large number of components and are complex, most raw materials are chemically synthesized sources and generally contain lead, mercury, hormones, preservatives, essence, pigments and the like, the components cannot play a role in skin care, the skin burden is increased, sensitive skin is overloaded, and symptoms such as red swelling, pruritus, desquamation and the like easily appear.

Therefore, it is important to develop a skin care product for sensitive skin, which has the functions of reducing irritation of sensitizers and repairing damaged barriers of sensitive skin.

Disclosure of Invention

Aiming at the defects in the prior art, the invention aims to provide an anti-allergy repairing agent prepared by mixing a stem cell extract and a compound plant extract; in addition, the invention also provides a preparation method of the anti-allergy repairing agent prepared by mixing the stem cell extract and the compound plant extract.

In order to solve the problems, the invention adopts the following technical scheme:

the invention provides an anti-allergy repairing agent prepared by mixing a stem cell extract and a compound plant extract, which comprises a skin stem cell whole extracting solution and the compound plant extract; the skin stem cell whole extracting solution is obtained by culturing human skin stem cells for more than 3 days, crushing and centrifuging the whole culture solution mixture of the stem cells, taking supernate, and filtering the supernate by a filter membrane; the complex plant extract includes centella asiatica extract, chamomilla extract, yarrow extract, purslane extract, and malva sylvestris extract.

Specifically, glycerol glucoside, trimethyl alanine, serine, allantoin and hyaluronic acid are also included.

Specifically, 900-1200 parts of skin stem cell total extract, 3-12 parts of centella asiatica extract, 3-10 parts of chamomile extract, 1-5 parts of achillea asiatica extract, 1-5 parts of purslane extract, 1-3 parts of malva sylvestris extract, 1-3 parts of glycerol glucoside, 1-2 parts of trimethyl glycine, 1-2 parts of serine, 0.5-1 part of allantoin and 0.1-1 part of hyaluronic acid.

Specifically, 1000 parts of a skin stem cell total extract, 12 parts of a centella asiatica extract, 8 parts of a chamomile extract, 3 parts of a yarrow extract, 3 parts of a purslane extract, 2 parts of a malva sylvestris extract, 2 parts of glycerol glucoside, 1 part of trimethyl glycine, 1 part of serine, 0.8 part of allantoin, and 0.5 part of hyaluronic acid.

Specifically, the preparation method of the skin stem cell whole extract comprises the following steps: culturing human skin stem cells in a culture medium for more than 3 days, collecting a culture solution mixture, cracking and breaking the stem cells in the culture solution mixture by using ultrasonic waves, centrifuging a broken product, and taking a supernatant; filtering the supernatant with a filter membrane with a pore diameter of 250 nm; the culture medium is basal culture medium DMEM/F12, human serum albumin 4mg/mL, vitamin C60ng/mL, L-glutamine 4mmol/mL, epidermal growth factor 10ng/mL, 2-mercaptoethanol 100 mu M, and streptomycin double antibody solution 10 mu M.

In a second aspect of the present invention, there is provided a method for preparing an anti-allergy repairing agent prepared by mixing a stem cell extract and a composite plant extract, for preparing the anti-allergy repairing agent prepared by mixing a stem cell extract and a composite plant extract, comprising the following steps:

(1) weighing corresponding weight of glycerol glucoside, trimethyl alanine, serine, allantoin and hyaluronic acid, and adding into the skin stem cell whole extract for mixing;

(2) adding the centella asiatica extract, the chamomile extract, the yarrow extract, the purslane extract and the malva sylvestris extract in the corresponding proportion into the mixed solution obtained in the step (1), and stirring and mixing uniformly to obtain the anti-allergy repairing agent.

Compared with the prior art, the invention has the beneficial effects that:

the selected components are safe, non-irritant and free of toxic and side effects, the components have obvious mutual synergistic effect, various skin sensitivity problems can be solved, the damaged barrier of sensitive skin can be effectively repaired, and the skin-care cream has the effects of resisting oxidation, accelerating circulation metabolism, enhancing the immunity of damaged skin and improving the sensitivity of skin; in addition, the invention does not add preservatives, emulsifiers, stabilizers, essences, pigments and the like, and has remarkable antiallergic, soothing and repairing effects.

Detailed Description

In the formula of the invention, the characteristics and functions of the components are as follows:

the main active ingredients of the invention are extracted liquid obtained by processing the whole culture solution after the stem cells of the human skin are cultured, wherein the extracted liquid is rich in growth factors secreted by the stem cells, other micromolecular active substances and other ingredients. In addition, the active ingredients of the stem cell extract can fully and efficiently promote the repair of skin cells damaged by sensitive skin, and the rejection influence caused by ingredients from different sources and ingredients generated by different types of cells is avoided.

The composite plant extract selected by the invention comprises a centella extract, a chamomile extract, a yarrow extract, a purslane extract and a malva sylvestris extract, and the five raw materials are compounded according to a specific proportion to obtain the composite plant extract which has obvious synergistic effect in the aspects of reducing the stimulation of a sensitization source and repairing the damaged barrier function of sensitive skin.

Skin which is easy to be allergic is generally dry and easy to be irritated, itchy and pricked by external stimuli, even to generate inflammation. Skin moisturization is therefore one of the most important bases for skin protection and restoration of skin barrier function. The humectant system with a natural structure is selected, so that the moisture retention of the skin can be rapidly improved, the moisture is firmly locked, the cell activity is stimulated, and the absorption of the skin on active molecules in the skin stem cell whole extracting solution and anti-inflammatory repair active ingredients in the composite plant extract can be improved, so that the anti-allergy capability and the repair capability of the skin are further improved. The glycerin glucoside, the cymene glycine and the serine are selected to be mutually matched for use in the humectant system. Glycerol glucoside is a substance formed by combining glycerol and glucose molecules by glycosidic bonds, and has the functions of moisturizing and resisting oxidation. Lanmethylglycine, also called amino acid heat preservation agent, is a natural structural substance zwitterionic moisture preservation component, can rapidly improve the moisture retention of skin, stimulate cell activity, and has the effects of keeping skin moist and smooth and preventing dryness and darkness. Serine, with triple moisturizing effect: (1) forming a protective film on the surface of the skin to prevent the water loss; (2) promoting the synthesis of ceramide and strengthening the connection of keratinocytes; (3) each serine grabs 15 times of water molecules, permeates into a skin basal layer, strengthens cell moisture, delays cell aging, and forms an efficient moisturizing system through the synergistic effect of the serine, the glycerol glucoside and the cymene glycine.

Example 1

Preparing a skin stem cell whole extracting solution:

preparation of a culture medium: basal medium DMEM/F12, human serum albumin 4mg/mL, vitamin C60ng/mL, L-glutamine 4mmol/mL, epidermal growth factor 10ng/mL, 2-mercaptoethanol 100 μ M, and streptomycin double antibody solution 10 μ M. The epidermal growth factor promotes the division and growth of the skin stem cells, vitamin C and 2-mercaptoethanol protect cell tissues from being oxidized and inactivated, a good division environment of the skin stem cells is provided, the streptomycin double-antibody solution inhibits the growth of bacteria in a culture medium, a sterile growth environment is provided for the skin stem cells, and sufficient energy is provided for the growth of the skin stem cells at the positions of human serum albumin, L-glutamine and human serum albumin. The skin stem cells can be rapidly increased, stably grown and high in purity by adopting the culture medium to culture the skin stem cells outside.

Culturing human skin stem cells in a culture medium for more than 3 days, collecting a culture solution mixture, cracking and breaking the stem cells in the culture solution mixture by using ultrasonic waves, centrifuging a broken product, and taking a supernatant; filtering the supernatant with a filter membrane with pore diameter of 250 nm.

In this example, the anti-allergic repairing agent prepared by mixing the stem cell extract and the compound plant extract is prepared:

(1) weighing 2 parts of glycerol glucoside, 1 part of trimethyl glycine, 1 part of serine, 0.8 part of allantoin and 0.5 part of hyaluronic acid, and adding the materials into 1000 parts of skin stem cell total extract for fully mixing;

(2) and (2) respectively adding 12 parts of centella asiatica extract, 8 parts of chamomile extract, 3 parts of milfoil extract, 3 parts of purslane extract and 2 parts of malva sylvestris extract in corresponding proportions into the mixed solution obtained in the step (1), and uniformly stirring and mixing to obtain the anti-allergy repairing agent.

Example 2

Preparation of whole extract of skin stem cells reference example 1.

In this example, the anti-allergic repairing agent prepared by mixing the stem cell extract and the compound plant extract is prepared:

(1) weighing 1 part of glycerol glucoside, 2 parts of trimethyl alanine, 2 parts of serine, 0.5 part of allantoin and 0.1 part of hyaluronic acid, and adding the materials into 1200 parts of a skin stem cell whole extracting solution for fully mixing;

(2) respectively adding 9 parts of centella extract, 3 parts of chamomile extract, 1 part of achillea extract, 1 part of purslane extract and 1 part of malva sylvestris extract in corresponding proportions into the mixed solution obtained in the step (1), and stirring and mixing uniformly to obtain the anti-allergy repairing agent.

Example 3

Preparation of whole extract of skin stem cells reference example 1.

In this example, the anti-allergic repairing agent prepared by mixing the stem cell extract and the compound plant extract is prepared:

(1) weighing 3 parts of glycerol glucoside, 1 part of trimethyl alanine, 1 part of serine, 1 part of allantoin and 1 part of hyaluronic acid, and adding the materials into 900 parts of skin stem cell total extract for fully mixing;

(2) respectively adding 3 parts of centella extract, 10 parts of chamomile extract, 5 parts of milfoil extract, 5 parts of purslane extract and 3 parts of malva sylvestris extract in corresponding proportions into the mixed solution obtained in the step (1), and uniformly stirring and mixing to obtain the anti-allergy repairing agent.

Test examples

Animal experiments with anti-allergic restoratives to promote skin regeneration and reduce scarring:

the experimental method comprises the following steps:

(1) purchasing 20 albino guinea pigs, male, 6 weeks old, breeding in a sterile room for 1 week, randomly dividing into 2 groups, which are respectively a control group (10), and an anti-allergy repairing agent treatment group (10);

(2) after anesthetizing the mice, shaving the right back, cutting off the circular skin with the diameter of 1cm, fixing the wound with a sterile suture to prevent wound contraction, and covering the wound with a dressing;

(3) anti-allergic repair agent treatment group the anti-allergic repair agent of example 1 was injected to the bottom side of the dressing at 400 μ L each. The control group was injected with placebo saline, but at the same dose, once every 3 days;

(4) the dressing is taken out every 30 days, the healing condition and the scar forming condition of the mouse wound surface are observed, and the examination is carried out after 3 months, the result shows that the healing degree of the mouse wound surface of the anti-allergy repairing agent treatment group is obviously higher than that of the control group, the scar repairing speed is higher, the scar volume of the mouse of the anti-allergy repairing agent treatment group is smaller than that of the control group, and the effect of inhibiting the scar forming can be effectively exerted.

Claims (6)

1. An anti-allergy repairing agent prepared by mixing a stem cell extract and a composite plant extract is characterized by comprising a skin stem cell whole extracting solution and the composite plant extract; the skin stem cell whole extracting solution is obtained by culturing human skin stem cells for more than 3 days, crushing and centrifuging the whole culture solution mixture of the stem cells, taking supernate, and filtering the supernate by a filter membrane; the complex plant extract includes centella asiatica extract, chamomilla extract, yarrow extract, purslane extract, and malva sylvestris extract.

2. The anti-allergy restoration agent prepared by mixing the stem cell extract and the composite plant extract according to claim 1, further comprising glycerol glucoside, trimethyl alanine, serine, allantoin and hyaluronic acid.

3. The anti-allergy restoration agent prepared by mixing the stem cell extract and the composite plant extract as claimed in claim 2, wherein the skin stem cell whole extract comprises 900-1200 parts of centella asiatica extract, 3-12 parts of chamomile extract, 1-5 parts of yarrow extract, 1-5 parts of purslane extract, 1-3 parts of malva sylvestris extract, 1-3 parts of glycerol glucoside, 1-2 parts of trimethyl glycine, 1-2 parts of serine, 0.5-1 part of allantoin and 0.1-1 part of hyaluronic acid.

4. The anti-allergy restoration agent prepared by mixing the stem cell extract and the composite plant extract according to claim 3, wherein the skin stem cell total extract comprises 1000 parts of skin stem cell total extract, 12 parts of centella asiatica extract, 8 parts of chamomile extract, 3 parts of achillea asiatica extract, 3 parts of purslane extract, 2 parts of malva sylvestris extract, 2 parts of glycerol glucoside, 1 part of trimethyl glycine, 1 part of serine, 0.8 part of allantoin and 0.5 part of hyaluronic acid.

5. The anti-allergy restoration agent prepared by mixing the stem cell extract and the compound plant extract according to any one of claims 1 to 4, wherein the skin stem cell whole extract is prepared by the following method: culturing human skin stem cells in a culture medium for more than 3 days, collecting a culture solution mixture, cracking and breaking the stem cells in the culture solution mixture by using ultrasonic waves, centrifuging a broken product, and taking a supernatant; filtering the supernatant with a filter membrane with a pore diameter of 250 nm; the culture medium is basal culture medium DMEM/F12, human serum albumin 0.1-5mg/mL, vitamin C35-80ng/mL, L-glutamine 1.5-5mmol/mL, epidermal growth factor 1-20ng/mL, 2-mercaptoethanol 25-120 mu M, and streptomycin double-antibody solution 2-15 mu M.

6. A method for preparing the anti-allergic repairing agent prepared by mixing the stem cell extract and the compound plant extract, which is used for preparing the anti-allergic repairing agent prepared by mixing the stem cell extract and the compound plant extract, as defined in claim 5, and is characterized by comprising the following steps:

(1) weighing corresponding weight of glycerol glucoside, trimethyl alanine, serine, allantoin and hyaluronic acid, and adding into the skin stem cell whole extract for mixing;

(2) adding the centella asiatica extract, the chamomile extract, the yarrow extract, the purslane extract and the malva sylvestris extract in the corresponding proportion into the mixed solution obtained in the step (1), and stirring and mixing uniformly to obtain the anti-allergy repairing agent.