CN105213291A - A kind of compound cold cream eliminating striae gravidarum and preparation method thereof - Google Patents
A kind of compound cold cream eliminating striae gravidarum and preparation method thereof Download PDFInfo
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- CN105213291A CN105213291A CN201510550402.3A CN201510550402A CN105213291A CN 105213291 A CN105213291 A CN 105213291A CN 201510550402 A CN201510550402 A CN 201510550402A CN 105213291 A CN105213291 A CN 105213291A
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- bacillus botulinus
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Landscapes
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Abstract
The invention discloses a kind of compound cold cream eliminating striae gravidarum and preparation method thereof, belong to cosmetics of everyday use and cosmetic products field, the technical scheme adopted is, a kind of compound cold cream eliminating striae gravidarum, comprise mixed uniformly following raw material: 60-100mL bacillus botulinus breaking cellular wall protein liquid, 5-15mL glycerol, 5-15mL vitamin E, 5-15mL vitamin A, 10-20g vitamin C, 15-25? g collagen protein, 10-25? g Radix Notoginseng powder, 10-25? g Poria powder, 2-10mL Herba Lysimachiae foenum-graeci quintessence oil, 0.1-10? g sodium carboxymethyl cellulose, bacillus botulinus breaking cellular wall protein liquid is the protein solution that bacillus botulinus culture fluid extracts after broken wall treatment, does is its protein content 0.3-0.5? wt.%.Its advantage is reasonable mixture ratio, effectively eliminates and alleviates striae gravidarum, and good biocompatibility, safety is high, and preparation method is simple, and Suitable commercial is produced.
Description
Technical field
The invention belongs to cosmetics of everyday use and cosmetic products field, particularly a kind of compound cold cream eliminating striae gravidarum and preparation method thereof.
Background technology
Striae gravidarum is the one of striae atrophicae, often appears at the places such as mother's stomach wall in puerperal, the interior outside of thigh, buttocks, chest, back lumbar part and arm, becomes their worries, not only affect wear the clothes dressing and the confidence of women, also affect social activity and the life of women.It is mainly due to trimester of pregnancy hormonal impact, in addition abdominal tympanites makes the elastic fibers of skin and collagen fiber damage or rupture, skin of abdomen is thinning to attenuate, there are some width difference, pink different in size or mauve wavy decorative patterns, divide the puerperium, these decorative patterns fade away, then leave white or argenteous glossiness cicatrix strain line, i.e. striae gravidarum, once occur that striae gravidarum would not be died away, and with cutis laxa, breast tenesmus, abdominal fat accumulation, had a strong impact on figure and the physical and mental health in women puerperal.
At present, the method for common elimination striae gravidarum mainly comprises: (1) quintessence oil or cream frost, the method for massage and instrument auxiliary treatment, the osmotic effect of quintessence oil is good, but does not have good repairing effect.By the reparation of a year, effect can reach about 40%, and the operating time is long, and current cream can only be desalinated, and the course for the treatment of is long, and effect is also undesirable; (2) laser therapy, this method is not suitable for large area treatment, and early stage can see effect, and stage does not change, and also require very high to operating technology, cost is high, and effect is undesirable; (3) skin method is changed, use Chemopeel agent, many use acidity or corrosive substance, patient suffering in process, also easily cause propagation cicatrix and occur, skin is thin and the skin 50% of sensitivity can cause propagation cicatrix, large area uses has harmful effect to health, Skin Cell is impaired, affects metabolism, and chemical substance causes skin pigmentation after producing precipitation.(4) inject Basic fibroblast growth factor, it promotes the process of granulation hyperplasia (being generally used for face to fill), and filling itself is held time short, technology should not be grasped, expense is large, can repair about 60% (5) filing, physics grinding skin painful and easily stay cicatrix.
As fully visible, daily skin-protection product or the method effect of repairing at present, eliminating striae gravidarum are all undesirable, are difficult problems urgently to be resolved hurrily on the people road of liking to be beautiful.
Summary of the invention
For solving the problems of the technologies described above, the invention provides a kind of compound cold cream eliminating striae gravidarum and preparation method thereof, adopt bacillus botulinus breaking cellular wall protein liquid, multivitamin, natural collagen protein, Chinese herbal medicine powder and natural plant extraction quintessence oil with science, rational proportion compound, and be made into the technical scheme of cold cream easy to use, achieve efficient elimination striae gravidarum and wrinkle, enhancing skin elasticity by its synergism, nourish skin whitening, and its preparation method is simple, be applicable to commercial production, easy to use.
The technical solution used in the present invention is, a kind of compound cold cream eliminating striae gravidarum, described cold cream comprises mixed uniformly following raw material: 60-100mL bacillus botulinus breaking cellular wall protein liquid, 5-15mL glycerol, 5-15mL vitamin E, 5-15mL vitamin A, 10-20g vitamin C, 15-25g collagen protein, 10-25g Radix Notoginseng powder, 10-25g Poria powder, 2-10mL Herba Lysimachiae foenum-graeci quintessence oil, 0.1-10g sodium carboxymethyl cellulose, described bacillus botulinus breaking cellular wall protein liquid is the protein solution that bacillus botulinus culture fluid extracts after broken wall treatment, and its protein content is 0.3-0.5wt.%.
The raw material of described cold cream also comprises 5-15g allantoin and 0.1-0.2g Mentholum.
Preferably, described cold cream is formed by following raw material Homogeneous phase mixing: 80-100mL bacillus botulinus breaking cellular wall protein liquid, 10-15mL glycerol, 10-15mL vitamin E, 10-15mL vitamin A, 15-20g vitamin C, 20-25g collagen protein, 22-25g Radix Notoginseng powder, 22-25g Poria powder, 10-15g allantoin, 0.1-0.15g Mentholum, 2-4mL Herba Lysimachiae foenum-graeci quintessence oil, 5-8g sodium carboxymethyl cellulose.
Described bacillus botulinus breaking cellular wall protein liquid is prepared as follows: (1) is got bacillus botulinus strain and is seeded in LB fluid medium by inoculum concentration 1 ring/1L or volume ratio 0.01-0.1%, and 37 DEG C of constant temperature culture 48-96h, obtain bacillus botulinus fermentation liquid; (2) the bacillus botulinus fermentation liquid in (1) is carried out cell breakage, centrifugally after 100 DEG C of water-bath detoxification treatment abandon precipitation, retain supernatant; (3) add ammonium sulfate to the supernatant in (2), centrifugal collecting precipitation, the precipitation of collection to be dialysed 18-30h with phosphate buffer, adjustment protein content is 0.3-0.5wt.%, obtains bacillus botulinus breaking cellular wall protein liquid.
The present invention also provides the preparation method of the compound cold cream of above-mentioned elimination striae gravidarum, and described preparation method comprises the following steps:
(1) bacillus botulinus breaking cellular wall protein liquid is prepared as stated above;
(2) the bacillus botulinus breaking cellular wall protein liquid 60-100mL in (1) is got, add 5-15mL glycerol, 5-15mL vitamin E, 5-15mL vitamin A, 10-20g vitamin C, 15-25g collagen protein, 10-25g Radix Notoginseng powder, 10-25g Poria powder, 2-10mL Herba Lysimachiae foenum-graeci quintessence oil and 0.1-10g sodium carboxymethyl cellulose while stirring successively, obtain mixture slurry;
(3) mixture slurry in (2) is stirred 20-40 minute, obtain compound cold cream
.
Preferably, described bacillus botulinus strain is that separation and purification obtains from the rotten bacon that rots, its separation and purification process is as follows: picking bacon rots surperficial microbionation in LB solid medium 37 DEG C of constant temperature culture 24-72h, picking list colony inoculation is on new LB solid medium, 37 DEG C of constant temperature culture 24-72h, basis of microscopic observation, repeatedly carry out picking list colony inoculation to new LB solid medium constant temperature culture, be bright flesh bacillus venenosus to basis of microscopic observation bacterium colony, namely screening obtains the bacillus botulinus strain of purification.
Preferably, described bacillus botulinus fermentation liquid carries out the centrifugal condition after cell breakage, 100 DEG C of water-bath detoxification treatment is 4500-5500r/min, 8-15min; Described add ammonium sulfate after to carry out centrifugal condition be 8000r/min-10000r/min, 8-15min.
Preferably, described in the process preparing bacillus botulinus breaking cellular wall protein liquid, dialysis procedure is: add 70-90mL phosphate buffer in 1L bacillus botulinus fermentation liquid gained precipitation, the buffer being dissolved with precipitation is put into bag filter, bag filter is put into phosphoric acid dialysis buffer liquid again, maintain and stir, every 4-6h changes a dialysis buffer liquid, dialysis 18-30h.
Preferably, in described step (2), Radix Notoginseng powder and/or Poria powder carry out pretreatment before addition, and described pretreatment comprises sterilizing, drying, pulverizing, and the Radix Notoginseng powder after pulverizing and/or Poria powder granularity are 200-300 order.
Preferably, in described step (2), Herba Lysimachiae foenum-graeci quintessence oil purity is more than 99%.
In technique scheme, bacillus botulinus breaking cellular wall protein liquid is the protein-contg solution liquid of richness extracted after the process of bacillus botulinus fermentation liquor cell breakage, the small molecular protein high containing purity, homogeneity is good, high effect nontoxic, can strengthen skin elasticity, irritation cell division and growth; Adding glycerol can make solution possess the fat-soluble and water miscible material of dissolving, to add effective ingredient; Collagen protein, preferred natural collagen protein, can improve the elastic force of skin fiber and the tension force of muscle and dilatability, strengthens cell wall thickness, the creotoxin protein liquid synergy of collagen protein and extraction is played and is controlled of flaccid muscles, keeps the effect of the elasticity of muscle and tension force; Vitamin E, vitamin A, vitamin C can improve with rational proportion and promote skin metabolism, increase skin activity, have significant defying age and antioxidation; Chinese medicine ingredients Radix Notoginseng powder, Poria powder are natural relaxing through Vasobral, Herba Lysimachiae foenum-graeci quintessence oil combined effect extract with pure natural plant can effectively defying age with increase the elasticity of muscle, the effect of potentiation can be played to bacillus botulinus breaking cellular wall protein liquid and collagen protein; Allantoin is a kind of amphoteric compound, double salt can be formed in conjunction with many kinds of substance, there is lucifuge, sterilization, anticorrosion, pain relieving, Promote cell's growth and antioxidation, can accelerating wound, horny layer softening albumen, alleviation and treatment xerosis cutis, make skin moisturizing, softness; Mentholum acts on skin or mucosa, has refrigerant itching-relieving action; Sodium carboxymethyl cellulose is used as thickening agent, is conducive to the paste body shape that product becomes easy to use; This product can rapid permeability skin cell, promotes the microcirculation of epidermis, and irritation cell is newborn, promotes cell oxygen content, increases the repairing in skin repair and wrinkle gap.
The invention has the beneficial effects as follows: (1) the present invention with small molecular protein and natural vitamin, collagen protein and Chinese herbal medicine powder, plants essential oil for main component, reasonable mixture ratio, preparation technology's science, by its synergism plurality of active ingredients potentiation each other, skin protection wrinkle-proof effect doubles to present, effective elimination and alleviate striae gravidarum, prevention skin aging and enhancing skin elasticity, to skin, there is the effects such as moist, pale, crease-resistant, also there is effects such as alleviating skin pruritus simultaneously, and good biocompatibility, safety is high; (2) cold cream preparation method provided by the invention is simple, and Suitable commercial is produced.
Below in conjunction with accompanying drawing, the present invention is described in detail.
Accompanying drawing explanation
Fig. 1 is the botulinal bacterium colony figure of separation and purification;
Fig. 2 is the thalli morphology figure under microscope in bacillus botulinus fermentation liquid;
Fig. 3 carries out the gel electrophoresis figure after SDS-page analysis to bacillus botulinus breaking cellular wall protein liquid, wherein, the rightmost side one is classified as the histogram of the protein molecular weight standard as reference, and left side three is classified as the histogram of the bacillus botulinus breaking cellular wall protein liquid sample of variable concentrations (from left to right concentration is followed successively by 1/6 concentration, 1/3 concentration, the rear protein liquid stock solution of dialysis);
Fig. 4 is the band-molecular weight corresponding diagram of protein molecular weight standard.
Detailed description of the invention
Elaborate to the present invention below by way of specific embodiment, wherein used experimental technique if no special instructions, is conventional method; Material used, reagent etc., if no special instructions, all can obtain from commercial channels.The present invention utilizes small molecular protein and natural vitamin, collagen protein and Chinese herbal medicine powder, plants essential oil to be main component, and reasonable compatibility makes cold cream, for alleviating and eliminating striae gravidarum, its compatibility example is as shown in table 1 below, bacillus botulinus breaking cellular wall protein liquid is wherein through the acquisition of following process, (1) get bacillus botulinus strain through LB solid medium slat chain conveyor, shaking flask LB fluid medium 37 DEG C of constant temperature culture 48-96h, obtain bacillus botulinus fermentation liquid; (2) by after the bacillus botulinus fermentation liquor cell breakage in (1), 100 DEG C of water-bath detoxification treatment, centrifugal cell wall of abandoning precipitates, and retains supernatant; (3) add ammonium sulfate to the supernatant in (2), centrifugal collecting precipitation, the precipitation of collecting to be dialysed 18-30h with phosphate buffer (pH6.8), adjustment protein content is 0.3-0.5wt.%, obtains bacillus botulinus breaking cellular wall protein liquid.Phosphate buffer used is pH6.8, and its compound method, for getting 0.2mol/L potassium dihydrogen phosphate 250mL, adds 0.2mol/L sodium hydroxide solution 118mL, is settled to 1L, shakes up with water.
The composition example of the compound cold cream of striae gravidarum eliminated by table 1
| Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | |
| Bacillus botulinus breaking cellular wall protein liquid/mL | 80 | 100 | 60 | 100 | 70 |
| Glycerol/mL | 10 | 15 | 5 | 10 | 12 |
| Vitamin E/mL | 10 | 15 | 5 | 10 | 8 |
| Vitamin A/mL | 10 | 15 | 5 | 5 | 8 |
| Vitamin C/g | 15 | 20 | 10 | 10 | 8 |
| Collagen protein/g | 20 | 25 | 15 | 25 | 18 |
| Radix Notoginseng powder/g | 20 | 12 | 10 | 15 | 22 |
| Poria powder/g | 20 | 12 | 10 | 15 | 22 |
| Allantoin/g | 10 | 15 | 0 | 5 | 12 |
| Mentholum/g | 0.15 | 0.2 | 0.1 | 0 | 0.18 |
| Herba Lysimachiae foenum-graeci quintessence oil/mL | 2 | 10 | 5 | 4 | 8 |
| Sodium carboxymethyl cellulose/g | 5 | 10 | 1 | 8 | 5 |
In mentioned component, first through pretreatment before Radix Notoginseng powder and/or Poria powder use, pre-treatment step comprises sterilizing, drying, pulverizing, and the granularity after pulverizing is 200-300 order, and Herba Lysimachiae foenum-graeci quintessence oil is that pure natural plant extraction obtains, and purity is more than 99%.
preparation embodiment
The preparation method of the compound cold cream of elimination striae gravidarum of the present invention is described for above-described embodiment 1:
(1) separation and purification bacillus botulinus strain:
Picking bacon rots surperficial microbionation in LB solid culture primary surface, 48h is cultivated under 37 DEG C of constant temperatures, with inoculating loop picking list colony inoculation on another new LB solid medium, 37 DEG C of constant temperature culture 24-72h, basis of microscopic observation colonial morphology, repeatedly carrying out the process of constant temperature culture on inoculating loop picking list colony inoculation to new LB solid medium, is bright flesh bacillus venenosus to basis of microscopic observation bacterium colony, and namely screening obtains the bacillus botulinus strain of purification.
(2) bacillus botulinus breaking cellular wall protein liquid is prepared:
1. with inoculating loop picking 1 ring bacillus botulinus strain inoculation in the fermentation tank filling 1LLB fluid medium, 37 DEG C of constant temperature culture 72h, obtain bacillus botulinus fermentation liquid; Wherein, the process for preparation of LB fluid medium is: add tryptone 10 grams, yeast extract 5 grams, 10 grams, sodium chloride in 950mL deionized water, shake to solute dissolves, adjusts pH to 7.0 with the NaOH of 5mol/L, 1L is settled to, finally 121 DEG C of steam sterilizations 20 minutes with deionized water;
2. the bacillus botulinus fermentation liquid in is 1. carried out ultrasonic cell-break, obtain cell breakage liquid, cell breakage liquid is put into 100 DEG C of water-baths to keep carrying out water-bath detoxification treatment in 30 minutes, mainly make other degeneration such as albumen, nucleic acid by high temperature, play detoxification and disinfective action.Cell breakage liquid after detoxification treatment, with the centrifugal 10min of 5000r/min, is abandoned cell wall precipitation, is retained supernatant; Wherein, cell breakage is except above-mentioned ultrasonic cell-break, colloid mill, superhigh-voltage homogenizing machine can also be selected to carry out cell breakage, when carrying out cell breakage with colloid mill, superhigh-voltage homogenizing machine, cultured bacillus botulinus fermentation liquid is poured in colloid mill or superhigh-voltage homogenizing machine, start running can be broken by bacillus botulinus, for ensureing that crushing effect can repeatedly broken 1-3 time, do not have complete cell to basis of microscopic observation; When carrying out cell breakage with ultrasound wave, cultured bacillus botulinus fermentation liquid is placed in beaker, beaker is placed in ice bath, under cryogenic, ultrasound probe is placed in bacillus botulinus fermentation liquid, carries out supersonic cell pulverizing, at interval of 10 ultrasonic seconds once, about ultrasonic time amounts to 10 minutes, checks do not have complete bacillus botulinus cell in microscope;
3. ammonium sulfate is added to the supernatant in 2., ammonium sulfate final concentration is made to reach 50%, centrifugal, 8000r/min-10000r/min, centrifugal 10min, collect rich protein-contg precipitation, abandon supernatant, the ratio that the protein precipitation of collection is dissolved in 80mL phosphate buffer (pH6.8) according to every 1L bacillus botulinus fermentation liquid is dissolved in phosphate buffer (pH6.8), then bag filter (specification 8000-14000 dalton is loaded, Viskase company of the U.S. produces), dialysis buffer liquid outside bag filter is similarly phosphate buffer, under agitation, dialyse 24 hours, a dialysis buffer liquid is changed every 6 hours, thoroughly to remove ammonium sulfate, after dialysis terminates, get protein liquid in bag filter conveniently operational approach carry out sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis, test sample divides three concentration, be respectively dialysis terminate the protein liquid stock solution in bag filter and add 2 times, the phosphate buffer (pH6.8) of 5 times of volumes is diluted to the protein liquid diluent of 1/3 and 1/6 concentration, with the protein molecular weight standard (PageRuler that ThermoFisherScientific company produces
tMprestainedProteinLadder, 10to180kDa, model 26616) be reference, the results are shown in accompanying drawing 3, contrast band is visible, and bacillus botulinus is after fragmentation, dialysis, molecular weight of albumen in the protein liquid collected is 23kd, to measure the protein concentration in the protein liquid collected further in conjunction with spectrophotometric analysis with Coomassie brilliant G-250 dyeing, and to adjust protein concentration be 0.3-0.5wt.%, as the bacillus botulinus breaking cellular wall protein liquid used during preparation cold cream of the present invention.
Wherein, the detailed process of coomassie brilliant blue staining is as follows:
A. crystallization bovine serum albumin or casein are chosen in reagent configuration, be 6.6 calculate protein percentage, be then mixed with the bovine serum albumin standard liquid that concentration is 100ug/mL with micro-Kjeldahl or according to the extinction coefficient of bovine serum albumin.Coomassie brilliant G-250 stock solution is dissolved in 50mL95% ethanol, add the phosphoric acid that 100mL mass concentration is 0.85g/mL, preserve as mother solution, 1000mL is diluted with water to during use, obtain Coomassie brilliant G-250 diluent, its final concentration is Coomassie brilliant G-250 mass concentration is 0.01%, phosphoric acid quality concentration is 0.085g/mL, for following use.
B. protein standard curve is drawn
Get 18 test tubes, be divided into 6 groups, often organize 3 as repeating to reduce error, according to the form below adds
| Test tube group # | 1 | 2 | 3 | 4 | 5 | 6 |
| 100ug/mL bovine serum albumin titer (mL) | 0 | 0.1 | 0.2 | 0.4 | 0.6 | 0.8 |
| Distilled water (mL) | 1.0 | 0.9 | 0.8 | 0.6 | 0.4 | 0.2 |
Then 5.0mL Coomassie brilliant G-250 diluent is added respectively to above-mentioned every test tube, shake up and place 5min, light absorption value is measured at 595nm place by ultraviolet-visible spectrophotometer, often to organize the light absorption value meansigma methods of test tube for vertical coordinate, with the quality of bovine serum albumin for abscissa draws protein standard curve.
C. protein sample gets 3 test tubes, often prop up the protein liquid added in the rear bag filter of 0.5mL dialysis, and adding distil water is supplemented to 1.0mL, separately get 3 test tubes to add 1.0mL distilled water respectively and do blank group, then 5.0mL Coomassie brilliant G-250 diluent is added to often propping up test tube, shake up after placing 5min, measure light absorption value at ultraviolet-visible spectrophotometer 595nm place.By the protein content of the protein liquid in the mean value calculation bag filter of the protein standard curve in B and measurement result, and adjust protein concentration, if to be the protein concentration that measures high for concrete method of adjustment, then directly to dilute with phosphate buffer; If concentration is low, then again with ammonium sulfate precipitation, centrifugal and dialyse, be 0.3-0.5wt.% to protein concentration.
(3) Radix Notoginseng powder and Poria powder carry out pretreatment before use, pretreated step comprises sterilizing, drying and pulverizes, granularity after pulverizing is 300 orders, get the bacillus botulinus breaking cellular wall protein liquid 80mL in (2), add glycerol, vitamin E, vitamin A, vitamin C, collagen protein, Radix Notoginseng powder, Poria powder, allantoin, Mentholum, Herba Lysimachiae foenum-graeci quintessence oil and sodium carboxymethyl cellulose successively while stirring according to proportioning shown in embodiment 1, obtain mixture slurry;
(4) by after the mixture slurry mixing in (3), on refiner, namely homogenate obtain the compound cold cream eliminating striae gravidarum and wrinkle half an hour, for ease of carrying and preserving, compound cold cream can be carried out subpackage, be sealed into fractional pack product, as 15g/ bottle, and 3 bottle/boxes.
application Example
Choose point women in-36 months June of puerperium 300, be divided into 30 groups on probation, every group 10 people, every little assembly beautiful tutor is responsible for instructing and tries out and be responsible for observing and record the curative effect situation that experimenter dispels striae gravidarum;
Using method: use respectively once in sooner or later each, preferably use after bathing, during use with hands picking cold cream slightly (about 0.5 gram) spread upon striae gravidarum or wrinkle of skin place and be evenly coated with and launch, exhibition position (massaging about five minutes) can be coated with have gentle hands forlement while painting exhibition, skin can be helped effectively to absorb by massage, improve product effect.One box is a course for the treatment of, uses to suspend after a course for the treatment of to use 7--10 days, then carries out next one course for the treatment of on probation, and the use effect of adding up after 3 courses for the treatment of is as follows altogether:
Comprehensive visible, the skin care item of elimination striae gravidarum provided by the invention and wrinkle, on probation through clinical 300 experimenters, wherein have 92.67%(278 people) experimenter, essentially eliminate striae gravidarum; 6.33%(19 people) experimenter, alleviate striae gravidarum; 1.00%(3 people) experimenter, slight alleviates striae gravidarum, simultaneously, this product can also alleviate wrinkle of skin effectively, the effect of prevention skin aging and enhancing skin elasticity, simultaneously also with alleviating the effects such as skin pruritus, has the effects such as moist, pale to skin.
Claims (10)
1. eliminate the compound cold cream of striae gravidarum for one kind, it is characterized in that, described cold cream comprises mixed uniformly following raw material: 60-100mL bacillus botulinus breaking cellular wall protein liquid, 5-15mL glycerol, 5-15mL vitamin E, 5-15mL vitamin A, 10-20g vitamin C, 15-25g collagen protein, 10-25g Radix Notoginseng powder, 10-25g Poria powder, 2-10mL Herba Lysimachiae foenum-graeci quintessence oil, 0.1-10g sodium carboxymethyl cellulose, described bacillus botulinus breaking cellular wall protein liquid is the protein solution extracted after bacillus botulinus fermentation liquor broken wall treatment, and its protein content is 0.3-0.5wt.%.
2. a kind of compound cold cream eliminating striae gravidarum according to claim 1, is characterized in that, the raw material of described cold cream also comprises 5-15g allantoin and 0.1-0.2g Mentholum.
3. a kind of compound cold cream eliminating striae gravidarum according to claim 1, it is characterized in that, described cold cream is formed by following raw material Homogeneous phase mixing: 80-100mL bacillus botulinus breaking cellular wall protein liquid, 10-15mL glycerol, 10-15mL vitamin E, 10-15mL vitamin A, 15-20g vitamin C, 20-25g collagen protein, 22-25g Radix Notoginseng powder, 22-25g Poria powder, 10-15g allantoin, 0.1-0.15g Mentholum, 2-4mL Herba Lysimachiae foenum-graeci quintessence oil, 5-8g sodium carboxymethyl cellulose.
4. according to the arbitrary described a kind of compound cold cream eliminating striae gravidarum of claim 1-3, it is characterized in that, described bacillus botulinus breaking cellular wall protein liquid is prepared as follows: (1) is got bacillus botulinus strain and is seeded in LB fluid medium by inoculum concentration 1 ring/1L or volume ratio 0.01-0.1%, 37 DEG C of constant temperature culture 48-96h, obtain bacillus botulinus fermentation liquid; (2) the bacillus botulinus fermentation liquid in (1) is carried out cell breakage, centrifugally after 100 DEG C of water-bath detoxification treatment abandon precipitation, retain supernatant; (3) add ammonium sulfate to the supernatant in (2), centrifugal collecting precipitation, the precipitation of collection to be dialysed 18-30h with phosphate buffer, adjustment protein content is 0.3-0.5wt.%, obtains bacillus botulinus breaking cellular wall protein liquid.
5. a kind of preparation method eliminating the compound cold cream of striae gravidarum according to claim 1, it is characterized in that, described preparation method comprises the following steps:
(1) bacillus botulinus breaking cellular wall protein liquid is prepared according to step described in claim 4;
(2) the bacillus botulinus breaking cellular wall protein liquid 60-100mL in (1) is got, add 5-15mL glycerol, 5-15mL vitamin E, 5-15mL vitamin A, 10-20g vitamin C, 15-25g collagen protein, 10-25g Radix Notoginseng powder, 10-25g Poria powder, 2-10mL Herba Lysimachiae foenum-graeci quintessence oil and 0.1-10g sodium carboxymethyl cellulose while stirring successively, obtain mixture slurry;
(3) mixture slurry in (2) is stirred 20-40 minute, obtain compound cold cream
.
6. a kind of preparation method eliminating the compound cold cream of striae gravidarum according to claim 5, it is characterized in that, described bacillus botulinus strain is that separation and purification obtains from the rotten bacon that rots, its separation and purification process is as follows: picking bacon rots surperficial microbionation in LB solid medium 37 DEG C of constant temperature culture 24-72h, picking list colony inoculation is on new LB solid medium, 37 DEG C of constant temperature culture 24-72h, basis of microscopic observation, repeatedly carry out picking list colony inoculation to new LB solid medium constant temperature culture, be bright flesh bacillus venenosus to basis of microscopic observation bacterium colony, namely screening obtains the bacillus botulinus strain of purification.
7. a kind of preparation method eliminating the compound cold cream of striae gravidarum according to claim 5, is characterized in that, the centrifugal condition that described bacillus botulinus fermentation liquid carries out after cell breakage, 100 DEG C of water-bath detoxification treatment is 4500-5500r/min, 8-15min; Described add ammonium sulfate after to carry out centrifugal condition be 8000r/min-10000r/min, 8-15min.
8. a kind of preparation method eliminating the compound cold cream of striae gravidarum according to claim 5, it is characterized in that, described dialysis procedure is: add 70-90mL phosphate buffer in 1L bacillus botulinus fermentation liquid gained precipitation, the buffer being dissolved with precipitation is put into bag filter, bag filter is put into phosphoric acid dialysis buffer liquid again, maintain and stir, every 4-6h changes a dialysis buffer liquid, dialysis 18-30h.
9. a kind of preparation method eliminating the compound cold cream of striae gravidarum according to claim 5, it is characterized in that, in described step (2), Radix Notoginseng powder and/or Poria powder carry out pretreatment before addition, described pretreatment comprises sterilizing, drying, pulverizing, and the Radix Notoginseng powder after pulverizing and/or Poria powder granularity are 200-300 order.
10. a kind of preparation method eliminating the compound cold cream of striae gravidarum according to claim 5, is characterized in that, in described step (2), Herba Lysimachiae foenum-graeci quintessence oil purity is more than 99%.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107375030A (en) * | 2017-08-02 | 2017-11-24 | 苏州爱喜邦物联网科技有限公司 | A kind of cold cream for eliminating striae of pregnancy |
| CN108057015A (en) * | 2018-01-12 | 2018-05-22 | 李忠 | A kind of striae of pregnancy repairs oil, preparation method and applications |
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| CN1416805A (en) * | 2001-11-07 | 2003-05-14 | 徐岑 | Natural Chinese medicine Yuzhu face nursing mask and its making process |
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| CN1416805A (en) * | 2001-11-07 | 2003-05-14 | 徐岑 | Natural Chinese medicine Yuzhu face nursing mask and its making process |
| CN103239387A (en) * | 2013-05-29 | 2013-08-14 | 张庆德 | Chinese traditional medicine face mask |
| CN103479565A (en) * | 2013-09-02 | 2014-01-01 | 吉安市御美丽健康产业有限公司 | Skin cream for removing striae gravidarum |
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| CN107375030A (en) * | 2017-08-02 | 2017-11-24 | 苏州爱喜邦物联网科技有限公司 | A kind of cold cream for eliminating striae of pregnancy |
| CN108057015A (en) * | 2018-01-12 | 2018-05-22 | 李忠 | A kind of striae of pregnancy repairs oil, preparation method and applications |
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