CN111235081B - 一种生物合成牛心果碱的微生物及其方法 - Google Patents

Abstract

本发明公开了一种生物合成牛心果碱的微生物及其方法,所述微生物不含有或者敲除dkgB、yeaE、yqhC、yqhD、dkgA、yahK和yjgB基因，高表达或者导入aroG^fbr、tyrA^fb、KDC、HpaB、HpaC、DODC、CAR、sfp、aldH、CNS、4OMT、6OMT和CNMT基因。本发明改造的微生物可实现由单糖或甘油到牛心果碱的微生物转化，为大规模的生物合成牛心果碱提供了一个可行道路。

Description

一种生物合成牛心果碱的微生物及其方法

技术领域

本发明属于微生物代谢工程领域，具体涉及改造微生物代谢途径，从单糖或甘油直接合成牛心果碱的方法。

背景技术

苄基异喹啉类生物碱包含2500多个种类,许多苄基异喹啉类生物碱，如:吗啡、小檗碱和诺斯卡品等在医学领域具有广阔的应用前景与经济地位。牛心果碱是苄基异喹啉类生物碱的共同中间体。

目前已商品化的苄基异喹啉类生物碱主要通过植物提取。由于植物生长周期长及苄基异喹啉类生物碱含量低，利用植物提取苄基异喹啉类生物碱成本较高。通过生物技术手段，实现微生物发酵单糖或甘油合成苄基异喹啉类生物碱共同中间体牛心果碱，可有效的降低成本，为规模化生物合成苄基异喹啉类生物碱提供了可行的道路。

发明内容

本发明的目的在于提供一种微生物体内合成牛心果碱的方法，在微生物内构建一条由单糖或甘油转化到牛心果碱的生物合成途径。

为实现上述目的，本发明提供了在大肠杆菌合成牛心果碱的方法，生物合成路径如图1所示，主要步骤为：

(1)敲除基因构建菌株DY

以大肠杆菌MG1655菌株出发，依次敲除该菌株中七个醛还原酶基因(dkgB、yeaE、yqhC、yqhD、dkgA、yahK和yjgB),敲除上述7个基因的菌株命名为DY。

(2)构建产牛心果碱工程菌DY1

将来自大肠杆菌的解反馈抑制aroG^fbr(3-deoxy-D-arabino heptulosonate-7-phosphate synthetase,aroG)基因和tyrA^fbr(chorismate mutase/prephenatedehydrogenase，tyrA)基因及来自酿酒酵母脱羧酶KDC基因，重组到载体pBBR1MCS1上，得到载体pDY001。将来自大肠杆菌的HpaBC(4-hydroxyphenylacetate 3-hydroxylase，HpaBC，由HpaB和HpaC组成)及经密码子优化人工合成的Pseudomonas putida菌DODC(DOPAdecarboxylase，DODC)、Papaver somniferum的CNS((S)-norcoclaurine synthase，CNS)、Papaver somniferum的6OMT((RS)-norcoclaurine 6-O-methyltransferase，6OMT)、Coptis japonica的4OMT(3'-hydroxy-N-methylcoclaurine4'-O-methyltransferase，4OMT)和Papaver somniferum的CNMT((S)-coclaurine N-methyltransferase，CNMT)基因，重组到载体pET28a(+)上，得到载体pDY002。将经密码子优化人工合成的Mycobacteriummarinum菌CAR(carboxylic acid reductase，CAR)、Bacillus subtilis subsp.subtilisstr.168菌的sfp(phosphopantetheinyl transferase,sfp)和大肠杆菌MG1655的aldH(aldehyde dehydrogenase，aldH)基因重组到载体pET28b-pSC101，得到载体pDY003。将载体pDY001、pDY002和pDY003转入到敲除七个醛还原酶基因的大肠杆菌DY中，实现上述12个基因在大肠杆菌中的高量表达，得到大肠杆菌工程菌株DY2。敲除七个醛还原酶基因用于阻断中间体4-羟基苯乙醛和3,4-二羟基苯乙醛被还原为副产物4-羟基苯乙醇和3,4-二羟基苯乙醇。表达解除反馈抑制的aroG^fbr基因和tyrA^fb基因用于加强大肠杆菌胞内对羟基苯丙酮酸和酪氨酸的合成。表达HpaB、HpaC、KDC、aldH和CAR用于催化对羟基苯丙酮酸转化为3,4二羟基苯乙醛。表达sfp用于活化CAR酶。表达HpaB、HpaC和DODC用于催化酪氨酸转化为3,4二羟基苯乙胺，表达CNS、6OMT、4OMT和CNMT用于随后催化3,4二羟基苯乙醛与3,4二羟基苯乙胺两个产物缩合修饰生成牛心果碱。上述构建的DY1工程菌可有效的将单糖或甘油转化为牛心果碱。

具体而言，本发明一方面涉及一种微生物，其特征在于所述微生物不含有或者敲除dkgB、yeaE、yqhC、yqhD、dkgA、yahK和yjgB基因，高表达或者导入aroG^fbr、tyrA^fb、KDC、HpaB、HpaC、DODC、CAR、sfp、aldH、CNS、4OMT、6OMT和CNMT基因。

进一步的，所述dkgB基因具有SEQ ID NO：1所示的核苷酸序列或者所述dkgB基因编码SEQ ID NO：2所示的氨基酸序列；

所述yeaE基因具有SEQ ID NO：3所示的核苷酸序列或者所述yeaE基因编码SEQ IDNO：4所示的氨基酸序列；

所述yqhC基因具有SEQ ID NO：5所示的核苷酸序列或者所述yqhC基因编码SEQ IDNO：6所示的氨基酸序列；

所述yqhD基因具有SEQ ID NO：7所示的核苷酸序列或者所述yqhD基因编码SEQ IDNO：8所示的氨基酸序列；

所述dkgA基因具有SEQ ID NO：9所示的核苷酸序列或者所述dkgA基因编码SEQ IDNO：10所示的氨基酸序列；

所述yahK基因具有SEQ ID NO：11所示的核苷酸序列或者所述yahK基因编码SEQID NO：12所示的氨基酸序列；

所述yjgB基因具有SEQ ID NO：13所示的核苷酸序列或者所述yjgB基因编码SEQID NO：14所示的氨基酸序列；

所述aroG^fbr基因具有SEQ ID NO：15所示的核苷酸序列或者所述aroG^fbr基因编码SEQ ID NO：16所示的氨基酸序列；

所述tyrA^fb基因具有SEQ ID NO：17所示的核苷酸序列或者所述tyrA^fb基因编码SEQ ID NO：18所示的氨基酸序列；

所述KDC基因具有SEQ ID NO：19所示的核苷酸序列或者所述KDC基因编码SEQ IDNO：20所示的氨基酸序列；

所述DODC基因具有SEQ ID NO：21所示的核苷酸序列或者所述DODC基因编码SEQID NO：22所示的氨基酸序列；

所述CNS基因具有SEQ ID NO：23所示的核苷酸序列或者所述CNS基因编码SEQ IDNO：24所示的氨基酸序列；

所述6OMT基因具有SEQ ID NO：25所示的核苷酸序列或者所述6OMT基因编码SEQID NO：26所示的氨基酸序列；

所述4OMT基因具有SEQ ID NO：27所示的核苷酸序列或者所述4OMT基因编码SEQID NO：28所示的氨基酸序列；

所述CNMT基因具有SEQ ID NO：29所示的核苷酸序列或者所述NMCH基因编码SEQID NO：30所示的氨基酸序列；

所述HpaB基因具有SEQ ID NO：31所示的核苷酸序列或者所述HpaB基因编码SEQID NO：32所示的氨基酸序列；

所述HpaC基因具有SEQ ID NO：33所示的核苷酸序列或者所述HpaC基因编码SEQID NO：34所示的氨基酸序列；

所述CAR基因具有SEQ ID NO：35所示的核苷酸序列或者所述CAR基因编码SEQ IDNO：36所示的氨基酸序列；

所述sfp基因具有SEQ ID NO：37所示的核苷酸序列或者所述sfp基因编码SEQ IDNO：38所示的氨基酸序列；

所述aldH基因具有SEQ ID NO：39所示的核苷酸序列或者所述aldH基因编码SEQID NO：40所示的氨基酸序列。

在本发明的一个优选实施方式中，微生物为大肠杆菌。

本发明另一方面还涉及上述微生物在生物合成牛心果碱中的应用。

作为微生物发酵发酵单糖或甘油合成牛心果碱的方法，所述的单糖为葡萄糖。

本发明的优点是在微生物中引入外源基因构建一条合成牛心果碱的代谢途径，最终实现以单糖或甘油为原料合成牛心果碱的目的，相比较与植物提取或化学合成可有效的降低成本。同时，微生物生长速度快，遗传操作技术成熟，且发酵过程不会对环境造成污染破坏。

附图说明

图1.牛心果碱生物合成路径图；

图2.大肠杆菌工程菌DY1和DY2发酵产物GC-MS检测结果。1号峰为内标4,4'-二甲氧基二苯甲酮,2号峰为产物牛心果碱。

图3，图2中发酵产物2号峰的离子碎片(上)与标准牛心果碱离子碎片(下)比较图。

具体实施方式

以下实施例用于进一步说明本发明，但不应理解为对本发明的限制。

实施例1构建产牛心果碱大肠杆菌工程菌

1.基因敲除菌株DY的构建

以pKD4质粒为模板，PCR扩增打靶片段,该片段包含(kan^r序列和与待敲除目的基因两端互补的同源臂)；将扩增的打靶片段电转到带有pKD46质粒的大肠杆菌中。利用pKD46质粒上带有的Red同源重组系统，使待敲除目的基因与打靶片段进行重组，目的基因被抗性基因取代。随后将温敏质粒pCP20导入菌株，利用该质粒上的FLP翻转酶去除目的菌株染色体上的抗性基因。利用上述方法依次敲除大肠杆菌MG1655菌株中的dkgB、yeaE、yqhC、yqhD、dkgA、yahK和yjgB基因，获得大肠杆菌DY。所述dkgB基因具有SEQ ID NO：1所示的核苷酸序列；所述dkgB基因具有与SEQ ID NO：2所示的氨基酸序列；所述yeaE基因具有SEQ ID NO：3所示的核苷酸序列；所述yeaE基因具有与SEQ ID NO：4所示的氨基酸序列；所述yqhC基因具有SEQ ID NO：5所示的核苷酸序列；所述yqhC基因具有与SEQ ID NO：6所示的氨基酸序列；所述yqhD基因具有SEQ ID NO：7所示的核苷酸序列；所述yqhD基因具有与SEQ ID NO：8所示的氨基酸序列；所述dkgA基因具有SEQ ID NO：9所示的核苷酸序列；所述dkgA基因具有与SEQ ID NO：10所示的氨基酸序列；所述yahK基因具有SEQ ID NO：11所示的核苷酸序列；所述yahK基因具有与SEQ ID NO：12所示的氨基酸序列；所述yjgB基因具有SEQ ID NO：13所示的核苷酸序列；所述yjgB基因具有与SEQ ID NO：14所示的氨基酸序列；敲除基因用的引物为：

DkgB-F:atggctatccctgcatttggtttaggtactttccgtctgagtgtaggctggagctgcttc

DkgB-R:ttaatcccattcaggagccagaccttccgggctaaccaggcggcatatgaatatcctcctta

yeaE-F:atgcaacaaaaaatgattcaatttagtggcgatgtctcactggtgtaggctggagctgcttc

yeaE-R:tcacaccatatccagcgcagtttttccttttggtgccggatatcatatgaatatcctcctta

yqhC-F:atgctacaaaattgcgcacaatcaaattgccgcattattcctaagtgtaggctggagctgcttc

yqhC-R:ttaattcccctgcatcgcccgcattcttgccgcatcttcccccatatgaatatcctcctta

yqhD-F:atgaacaactttaatctgcacaccccaacccgcattctgttgtgtaggctggagctgcttc

yqhD-R:ttagcgggcggcttcgtatatacggcggctgacatccaacgcatatgaatatcctcctta

dkgA-F：atggctaatccaaccgttattaagctacaggatggcaatgtcgtgtaggctggagctgcttc

dkgA-R：ttagccgccgaactggtcaggatcgggaccgagacgcttgccatatgaatatcctcctta

yahK-F:atgaagatcaaagctgttggtgcatattccgctaaacaaccgtgtaggctggagctgcttc

yahK-R:tcagtctgttagtgtgcgattatcgataacaaaacgatattcatatgaatatcctcctta

yjgB-F:atgtcgatgataaaaagctatgccgcaaaagaagcgggcggcgtgtaggctggagctgcttc

yjgB-R:tcaaaaatcggctttcaacaccacgcggtaacgcgccttaccgtcatatgaatatcctcctta

2.载体pDY001的构建

解除反馈抑制的aroG^fbr和tyrA^fbr基因委托金唯智生物科技有限公司合成，以合成的基因为模板利用引物aroG^fbr-XbaI(gtatctagaaagaggagatataatgaattatcagaacgacgatttacgc)和aroG^fbr-SpeI-BamHI(tatggatccactagtttacccgcgacgcgctttta)PCR扩增aroG^fbr基因，随后将扩增的片段以XbaI和BamHI插入到pET28a(+)中得到载体pDY1。以合成的基因为模板，利用引物tyrA^fbrr-XbaI(gtatctagaaagaggagatataatggttgctgaattgaccgcat)和tyrA^fbr-SpeI-SalI(atatgtcgacactagtttactggcgattgtcattcgcc)PCR扩增来tyrA^fbr基因，随后将扩增的片段以XbaI和SalI双酶切，插入到以SpeI和SalI双酶切的载体pDY1上，中得到载体pDY2。利用引物KDC-XbaI(atctctagatttaagaaggagatataatggcacctgttacaattgaaaagttc)和KDC-NheI-BamHI(tctggatccgctagcctattttttatttcttttaagtgccgctg)PCR扩增来自酿酒酵母菌YPH499的KDC基因，随后将扩增的片段以XbaI和BamHI双酶切,将其插入以XbaI和BamHI双酶切的载体pBBR1MCS1上，得到载体pDY3。利用XbaI和SalI双酶切pDY2得到aroG^fbr和tyrA^fbr表达框，将其插入以NheI和XhoI双酶切的载体pDY3，得到载体pDY001。所述aroG^fbr基因具有SEQ ID NO：15所示的核苷酸序列；所述aroG^fbr基因具有与SEQ ID NO：16所示的氨基酸序列；所述tyrA^fb基因具有SEQ ID NO：17所示的核苷酸序列编码；所述tyrA^fb基因具有SEQ ID NO：18所示的氨基酸序列；所述KDC基因具有SEQ ID NO：19所示的核苷酸序列编码；所述KDC基因具有SEQ ID NO：20所示的氨基酸序列。

3.载体pDY002的构建

DODC、CNS、6OMT、4OMT和CNMT基因委托金唯智生物科技有限公司合成。以合成的基因为模板，利用引物DODC-XbaI(tagtctagatttaagaaggagatataatgacccccgaacagttccg)/DODC-SpeI-BamHI(acaggatccactagtttagctgccgcctttaatcacgt)、CNS-XbaI(tactctagatttaagaaggagatataatgcgtaaagtgatcaaatatgatatgg)/CNS-SpeI-BamHI(tcaggatccactagtttagctgcccagtaaacgaggaa)、6OMT-XbaI(ttgtctagatttaagaaggagatataatggagaccgttagcaaaattgacc)/6OMT-SpeI-BamHI(gcaggatccactagtttagtacggatatgcttcaatcacgct)、4OMT-XbaI(tactctagatttaagaaggagatataatggccttccatggcaaggac)/4OMT-SpeI-BamHI(tcaggatccactagtttaaggaaacacctcaatcacgctct)、CNMT-XbaI(atctctagatttaagaaggagatataatgcagttaaaagaaaaagaggagctg)/CNMT-SpeI-BamHI(agaggatccactagtttattttttcttaaacagcagatgggttaac)，分别PCR扩增DODC、CNS、6OMT、4OMT和CNMT基因，随后将扩增的片段以XbaI和BamHI插入到pET28a(+)中，依次得到载体pDY4、pDY5、pDY6、pDY7和pDY8。利用XbaI和XhoI双酶切pDY5得到CNS表达框，将其插入以SpeI和XhoI双酶切的载体pDY4上，得到载体pDY9。利用XbaI和XhoI双酶切pDY6得到6OMT表达框，将其插入以SpeI和XhoI双酶切的载体pDY9上，得到载体pDY10。利用XbaI和XhoI双酶切pDY7得到4OMT表达框，将其插入以SpeI和XhoI双酶切的载体pDY10上，得到载体pDY11。利用XbaI和XhoI双酶切pDY8得到CNMT表达框，将其插入以SpeI和XhoI双酶切的载体pDY11上，得到载体pDY12。以大肠杆菌MG1655的基因组为模板，利用引物HpaBC-XbaI(tagtctagatttaagaaggagatataatgaaaccagaagatttccgcg)和HpaBC-XhoI(aacgctcgagttaaatcgcagcttccatttcca),PCR扩增HpaBC基因(包含HpaB和HpaC两个基因)，随后将扩增的片段以XbaI和XhoI双酶切,将其插入以SpeI和XhoI双酶切的载体pDY12上，得到载体pDY002。所述DODC基因具有SEQ ID NO：21所示的核苷酸序列；所述DODC基因具有与SEQ ID NO：22所示的氨基酸序列；所述CNS基因具有SEQ ID NO：23所示的核苷酸序列；所述CNS基因具有与SEQ ID NO：24所示的氨基酸序列；所述6OMT基因具有SEQ IDNO：25所示的核苷酸序列；所述6OMT基因具有与SEQ ID NO：26所示的氨基酸序列；所述4OMT基因具有SEQ ID NO：27所示的核苷酸序列；所述4OMT基因具有与SEQ ID NO：28所示的氨基酸序列；所述CNMT基因具有SEQ ID NO：29所示的核苷酸序列；所述CNMT基因具有与SEQ IDNO：30所示的氨基酸序列；所述HpaB基因具有SEQ ID NO：31所示的核苷酸序列；所述HpaB基因具有与SEQ ID NO：32所示的氨基酸序列；所述HpaC基因具有SEQ ID NO：33所示的核苷酸序列；所述HpaC基因具有与SEQ ID NO：34所示的氨基酸序列；

4.载体pDY003的构建

CAR基因委托金唯智生物科技有限公司合成。以合成的基因为模板，利用引物CAR-XbaI(atctctagatttaagaaggagatataatgagtccgatcacacgcgag)/CAR-SpeI-BamHI(agaggatccactagtttacagcaggcctaacaggcg)PCR扩增CAR基因，随后将扩增的片段以XbaI和BamHI双酶切，插入到以XbaI和BamHI双酶切的pET28a(+)中，得到载体pDY13。以芽孢杆菌Bacillussubtilis subsp.subtilisstr.168的基因组为模板，利用引物sfp-XbaI(gtctctagatttaagaaggagatataatgaagatttacggaatttatatggaccg)/sfp-SpeI-BamHI(tgaggatccactagtttataaaagctcttcgtacgagaccattgt)PCR扩增sfp基因，随后将扩增的片段以XbaI和BamHI双酶切，插入到以XbaI和BamHI双酶切的pET28a(+)中，得到载体pDY14。以大肠杆菌MG1655的基因组为模板，利用引物aldH-XbaI(atctctagatttaagaaggagatataatgaattttcatcatctggcttactg)/aldH-NheI-BamHI(tcaggatccgctagctcaggcctccaggctatcc)PCR扩增aldH基因，随后将扩增的片段以XbaI和BamHI双酶切，插入到以XbaI和BamHI双酶切的pET28a(+)中，得到载体pDY15。利用XbaI和XhoI双酶切pDY14得到sfp表达框，将其插入以SpeI和XhoI双酶切的载体pDY13上，得到载体pDY16。利用XbaI和XhoI双酶切pDY15得到aldH表达框，将其插入以SpeI和XhoI双酶切的载体pDY16上，得到载体pDY17。利用XbaI和XhoI双酶切pDY17得到CAR、sfp和aldH表达框，将其插入以XbaI和XhoI双酶切的载体pET28b-pSC101上，得到载体pDY003。所述CAR基因具有SEQ ID NO：35所示的核苷酸序列；所述CAR基因具有与SEQ IDNO：36所示的氨基酸序列；所述sfp基因具有SEQ ID NO：37所示的核苷酸序列；所述sfp基因具有与SEQ ID NO：38所示的氨基酸序列；所述aldH基因具有SEQ ID NO：39所示的核苷酸序列；所述aldH基因具有与SEQ ID NO：40所示的氨基酸序列；

5.将重组载体pDY001和pDY002同时导入大肠杆菌DY中，得到DY1工程菌。该工程菌可实现aroG^fbr、tyrA^fbr、KDC、HpaBC、DODC、CNS、6OMT、4OMT和CNMT基因的高表达，可有效的将单糖或甘油转化为牛心果碱。

5.将重组载体pDY001、pDY002和pDY003同时导入大肠杆菌DY中，得到DY2工程菌。该工程菌可实现aroG^fbr、tyrA^fbr、KDC、HpaBC、DODC、CNS、6OMT、4OMT、CNMT、CAR、sfp和aldH基因的高表达。相较于DY1工程菌，进一步实现CAR、sfp和aldH基因高效表达的DY2工程菌，可提升的将单糖或甘油转化为牛心果碱的生物合成效率。

实施例2大肠杆菌工程菌DY1生产牛心果碱的发酵实验

1、将37度LB中过夜培养的大肠杆菌工程菌DY1，接种到50ml M9发酵培养基中(包含2％葡萄糖)，进行30度摇瓶发酵，待菌体OD₆₀₀为0.8左右，加入0.1mM IPTG进行目的基因的诱导表达。

2、发酵培养24小时后收集菌体，超声波破细胞，利用氯仿萃取产物。

3、利用旋转蒸发仪将溶剂氯仿旋干，再用一定量的氯仿溶解产物。

4、GC-MS(气相色谱-质谱联用仪)检测样品。

5、实验结果：大肠杆菌工程菌利用葡萄糖合成牛心果碱的GC-MS检测结果如图2A所示，牛心果碱GC-MS鉴定如图3所示，表明菌株DY1可有效利用葡萄糖或甘油合成牛心果碱。

实施例3大肠杆菌工程菌DY2生产牛心果碱的发酵实验

1、将37度LB中过夜培养的大肠杆菌工程菌DY2，接种到50ml M9发酵培养基中(包含2％葡萄糖)，进行30度摇瓶发酵，待菌体OD₆₀₀为0.8左右，加入0.1mM IPTG进行目的基因的诱导表达。

2、发酵培养24小时后收集菌体，超声波破细胞，利用氯仿萃取产物。

3、利用旋转蒸发仪将溶剂氯仿旋干，再用一定量的氯仿溶解产物。

4、GC-MS(气相色谱-质谱联用仪)检测样品。

5、实验结果：大肠杆菌工程菌DY2利用葡萄糖合成牛心果碱的GC-MS检测结果如图2B，表明菌株DY2可有效利用葡萄糖或甘油合成牛心果碱。

以上所述仅是本发明的优选实施方案，应当指出，对于本技术领域的技术人员，在不脱离本分明原理的前提下，还可以做出适当的改进，这些改进也应视为本发明的保护范围。

序列表

<110> 赣南师范大学

<120> 一种生物合成牛心果碱的微生物及其方法

<160> 78

<170> SIPOSequenceListing 1.0

<210> 1

<211> 804

<212> DNA

<213> Unkown

<400> 1

atggctatcc ctgcatttgg tttaggtact ttccgtctga aagacgacgt tgttatttca 60

tctgtgataa cggcgcttga acttggttat cgcgcaattg ataccgcaca aatctatgat 120

aacgaagccg cagtaggtca ggcgattgca gaaagtggcg tgccacgtca tgaactctac 180

atcaccacta aaatctggat tgaaaatctc agcaaagaca aattgatccc aagtctgaaa 240

gagagcctgc aaaaattgcg taccgattat gttgatctga cgctaatcca ctggccgtca 300

ccaaacgatg aagtctctgt tgaagagttt atgcaggcgc tgctggaagc caaaaaacaa 360

gggctgacgc gtgagatcgg tatttccaac ttcacgatcc cgttgatgga aaaagcgatt 420

gctgctgttg gtgctgaaaa catcgctact aaccagattg aactctctcc ttatctgcaa 480

aaccgtaaag tggttgcctg ggctaaacag cacggcatcc atattacttc ctatatgacg 540

ctggcgtatg gtaaggccct gaaagatgag gttattgctc gtatcgcagc taaacacaat 600

gcgactccgg cacaagtgat tctggcgtgg gctatggggg aaggttactc agtaattcct 660

tcttctacta aacgtaaaaa cctggaaagt aatcttaagg cacaaaattt acagcttgat 720

gccgaagata aaaaagcgat cgccgcactg gattgcaacg accgcctggt tagcccggaa 780

ggtctggctc ctgaatggga ttaa 804

<210> 2

<211> 267

<212> PRT

<213> Unkown

<400> 2

Met Ala Ile Pro Ala Phe Gly Leu Gly Thr Phe Arg Leu Lys Asp Asp

1 5 10 15

Val Val Ile Ser Ser Val Ile Thr Ala Leu Glu Leu Gly Tyr Arg Ala

20 25 30

Ile Asp Thr Ala Gln Ile Tyr Asp Asn Glu Ala Ala Val Gly Gln Ala

35 40 45

Ile Ala Glu Ser Gly Val Pro Arg His Glu Leu Tyr Ile Thr Thr Lys

50 55 60

Ile Trp Ile Glu Asn Leu Ser Lys Asp Lys Leu Ile Pro Ser Leu Lys

65 70 75 80

Glu Ser Leu Gln Lys Leu Arg Thr Asp Tyr Val Asp Leu Thr Leu Ile

85 90 95

His Trp Pro Ser Pro Asn Asp Glu Val Ser Val Glu Glu Phe Met Gln

100 105 110

Ala Leu Leu Glu Ala Lys Lys Gln Gly Leu Thr Arg Glu Ile Gly Ile

115 120 125

Ser Asn Phe Thr Ile Pro Leu Met Glu Lys Ala Ile Ala Ala Val Gly

130 135 140

Ala Glu Asn Ile Ala Thr Asn Gln Ile Glu Leu Ser Pro Tyr Leu Gln

145 150 155 160

Asn Arg Lys Val Val Ala Trp Ala Lys Gln His Gly Ile His Ile Thr

165 170 175

Ser Tyr Met Thr Leu Ala Tyr Gly Lys Ala Leu Lys Asp Glu Val Ile

180 185 190

Ala Arg Ile Ala Ala Lys His Asn Ala Thr Pro Ala Gln Val Ile Leu

195 200 205

Ala Trp Ala Met Gly Glu Gly Tyr Ser Val Ile Pro Ser Ser Thr Lys

210 215 220

Arg Lys Asn Leu Glu Ser Asn Leu Lys Ala Gln Asn Leu Gln Leu Asp

225 230 235 240

Ala Glu Asp Lys Lys Ala Ile Ala Ala Leu Asp Cys Asn Asp Arg Leu

245 250 255

Val Ser Pro Glu Gly Leu Ala Pro Glu Trp Asp

260 265

<210> 3

<211> 855

<212> DNA

<213> Unkown

<400> 3

atgcaacaaa aaatgattca atttagtggc gatgtctcac tgccagccgt agggcaggga 60

acatggtata tgggcgaaga tgccagtcag cgcaaaacag aagttgctgc actacgcgcg 120

ggcattgaac tcggtttaac cctcattgat accgccgaaa tgtatgccga tggcggtgcc 180

gaaaaggtgg ttggggaagc attaaccggt ctgcgagaga aggtctttct cgtctctaaa 240

gtctatccgt ggaatgctgg cgggcaaaaa gcgataaatg catgcgaagc cagtttacgc 300

cgtctcaata ctgattatct cgatctttac ttattacact ggtctggcag tttcgctttt 360

gaagagactg tcgcagcgat ggaaaaattg atcgcccagg gaaaaatccg ccgctggggc 420

gtttctaacc ttgattatgc tgatatgcag gaactctggc agctgccggg gggaaatcag 480

tgtgccacta atcaggtgct ttaccatctc ggttcacgag gaattgagta cgatctactc 540

ccctggtgcc agcaacagca gatgccggtg atggcttaca gtccgttagc ccaggccggg 600

cggttgcgca atggactgtt aaaaaacgcg gtagtcaacg aaattgcaca tgctcacaat 660

atcagcgcgg cacaagtatt gttggcgtgg gtgatcagtc atcagggtgt gatggcgatt 720

ccaaaagcgg ccacgattgc ccatgtccaa caaaatgcgg ctgtgcttga ggtcgaactt 780

tcttcagcgg aattagctat gctggataag gcatatccgg caccaaaagg aaaaactgcg 840

ctggatatgg tgtga 855

<210> 4

<211> 284

<212> PRT

<213> Unkown

<400> 4

Met Gln Gln Lys Met Ile Gln Phe Ser Gly Asp Val Ser Leu Pro Ala

1 5 10 15

Val Gly Gln Gly Thr Trp Tyr Met Gly Glu Asp Ala Ser Gln Arg Lys

20 25 30

Thr Glu Val Ala Ala Leu Arg Ala Gly Ile Glu Leu Gly Leu Thr Leu

35 40 45

Ile Asp Thr Ala Glu Met Tyr Ala Asp Gly Gly Ala Glu Lys Val Val

50 55 60

Gly Glu Ala Leu Thr Gly Leu Arg Glu Lys Val Phe Leu Val Ser Lys

65 70 75 80

Val Tyr Pro Trp Asn Ala Gly Gly Gln Lys Ala Ile Asn Ala Cys Glu

85 90 95

Ala Ser Leu Arg Arg Leu Asn Thr Asp Tyr Leu Asp Leu Tyr Leu Leu

100 105 110

His Trp Ser Gly Ser Phe Ala Phe Glu Glu Thr Val Ala Ala Met Glu

115 120 125

Lys Leu Ile Ala Gln Gly Lys Ile Arg Arg Trp Gly Val Ser Asn Leu

130 135 140

Asp Tyr Ala Asp Met Gln Glu Leu Trp Gln Leu Pro Gly Gly Asn Gln

145 150 155 160

Cys Ala Thr Asn Gln Val Leu Tyr His Leu Gly Ser Arg Gly Ile Glu

165 170 175

Tyr Asp Leu Leu Pro Trp Cys Gln Gln Gln Gln Met Pro Val Met Ala

180 185 190

Tyr Ser Pro Leu Ala Gln Ala Gly Arg Leu Arg Asn Gly Leu Leu Lys

195 200 205

Asn Ala Val Val Asn Glu Ile Ala His Ala His Asn Ile Ser Ala Ala

210 215 220

Gln Val Leu Leu Ala Trp Val Ile Ser His Gln Gly Val Met Ala Ile

225 230 235 240

Pro Lys Ala Ala Thr Ile Ala His Val Gln Gln Asn Ala Ala Val Leu

245 250 255

Glu Val Glu Leu Ser Ser Ala Glu Leu Ala Met Leu Asp Lys Ala Tyr

260 265 270

Pro Ala Pro Lys Gly Lys Thr Ala Leu Asp Met Val

275 280

<210> 5

<211> 957

<212> DNA

<213> Unkown

<400> 5

atgctacaaa attgcgcaca atcaaattgc cgcattattc ctaagaaatt acgcgatatg 60

aaacgtgaag agatttgccg cttgctggcg gataaagtta ataaactgaa aaataaagaa 120

aatagtttgt caggactgtt gcccgatgtg cgtttgttgt atggcgagac gcctttcgca 180

cgtacaccgg tgatgtacga gcctggcatc ataattctct tttccgggca taaaatcggt 240

tatatcaatg aacgcgtgtt tcgttatgat gccaatgaat acctgctgct gacggtgccg 300

ttgccgtttg agtgcgaaac ctatgccacg tcagaggtgc cgctggcagg gttgcgtctc 360

aatgtcgata ttttgcagtt acaggaactg ttgatggaca ttggcgaaga tgagcatttc 420

cagccgtcga tggcagccag cgggattaac tccgccacgt tatcagaaga gattttatgc 480

gcggcggagc ggttactcga cgtgatggag cgaccactgg atgcgcgtat tctcggcaaa 540

cagatcatcc gcgaaattct gtactacgtg ctgaccggac cttgcggcgg cgcgttactg 600

gcgctggtca gtcgccagac tcacttcagt ctgattagcc gcgtgctgaa acggattgag 660

aataaataca ccgaaaacct gagcgtcgag caactggcgg cagaagccaa catgagcgta 720

tcggcgttcc accataattt taagtctgtc accagtacct cgccgttgca gtatttgaag 780

aattaccgtc tgcataaggc gcggatgatg atcatccatg acggcatgaa ggccagcgca 840

gcagcgatgc gcgtcggcta tgaaagcgca tcgcaattta gccgtgagtt taaacgttac 900

ttcggtgtga cgccggggga agatgcggca agaatgcggg cgatgcaggg gaattaa 957

<210> 6

<211> 318

<212> PRT

<213> Unkown

<400> 6

Met Leu Gln Asn Cys Ala Gln Ser Asn Cys Arg Ile Ile Pro Lys Lys

1 5 10 15

Leu Arg Asp Met Lys Arg Glu Glu Ile Cys Arg Leu Leu Ala Asp Lys

20 25 30

Val Asn Lys Leu Lys Asn Lys Glu Asn Ser Leu Ser Gly Leu Leu Pro

35 40 45

Asp Val Arg Leu Leu Tyr Gly Glu Thr Pro Phe Ala Arg Thr Pro Val

50 55 60

Met Tyr Glu Pro Gly Ile Ile Ile Leu Phe Ser Gly His Lys Ile Gly

65 70 75 80

Tyr Ile Asn Glu Arg Val Phe Arg Tyr Asp Ala Asn Glu Tyr Leu Leu

85 90 95

Leu Thr Val Pro Leu Pro Phe Glu Cys Glu Thr Tyr Ala Thr Ser Glu

100 105 110

Val Pro Leu Ala Gly Leu Arg Leu Asn Val Asp Ile Leu Gln Leu Gln

115 120 125

Glu Leu Leu Met Asp Ile Gly Glu Asp Glu His Phe Gln Pro Ser Met

130 135 140

Ala Ala Ser Gly Ile Asn Ser Ala Thr Leu Ser Glu Glu Ile Leu Cys

145 150 155 160

Ala Ala Glu Arg Leu Leu Asp Val Met Glu Arg Pro Leu Asp Ala Arg

165 170 175

Ile Leu Gly Lys Gln Ile Ile Arg Glu Ile Leu Tyr Tyr Val Leu Thr

180 185 190

Gly Pro Cys Gly Gly Ala Leu Leu Ala Leu Val Ser Arg Gln Thr His

195 200 205

Phe Ser Leu Ile Ser Arg Val Leu Lys Arg Ile Glu Asn Lys Tyr Thr

210 215 220

Glu Asn Leu Ser Val Glu Gln Leu Ala Ala Glu Ala Asn Met Ser Val

225 230 235 240

Ser Ala Phe His His Asn Phe Lys Ser Val Thr Ser Thr Ser Pro Leu

245 250 255

Gln Tyr Leu Lys Asn Tyr Arg Leu His Lys Ala Arg Met Met Ile Ile

260 265 270

His Asp Gly Met Lys Ala Ser Ala Ala Ala Met Arg Val Gly Tyr Glu

275 280 285

Ser Ala Ser Gln Phe Ser Arg Glu Phe Lys Arg Tyr Phe Gly Val Thr

290 295 300

Pro Gly Glu Asp Ala Ala Arg Met Arg Ala Met Gln Gly Asn

305 310 315

<210> 7

<211> 1164

<212> DNA

<213> Unkown

<400> 7

atgaacaact ttaatctgca caccccaacc cgcattctgt ttggtaaagg cgcaatcgct 60

ggtttacgcg aacaaattcc tcacgatgct cgcgtattga ttacctacgg cggcggcagc 120

gtgaaaaaaa ccggcgttct cgatcaagtt ctggatgccc tgaaaggcat ggacgtgctg 180

gaatttggcg gtattgagcc aaacccggct tatgaaacgc tgatgaacgc cgtgaaactg 240

gttcgcgaac agaaagtgac tttcctgctg gcggttggcg gcggttctgt actggacggc 300

accaaattta tcgccgcagc ggctaactat ccggaaaata tcgatccgtg gcacattctg 360

caaacgggcg gtaaagagat taaaagcgcc atcccgatgg gctgtgtgct gacgctgcca 420

gcaaccggtt cagaatccaa cgcaggcgcg gtgatctccc gtaaaaccac aggcgacaag 480

caggcgttcc attctgccca tgttcagccg gtatttgccg tgctcgatcc ggtttatacc 540

tacaccctgc cgccgcgtca ggtggctaac ggcgtagtgg acgcctttgt acacaccgtg 600

gaacagtatg ttaccaaacc ggttgatgcc aaaattcagg accgtttcgc agaaggcatt 660

ttgctgacgc taatcgaaga tggtccgaaa gccctgaaag agccagaaaa ctacgatgtg 720

cgcgccaacg tcatgtgggc ggcgactcag gcgctgaacg gtttgattgg cgctggcgta 780

ccgcaggact gggcaacgca tatgctgggc cacgaactga ctgcgatgca cggtctggat 840

cacgcgcaaa cactggctat cgtcctgcct gcactgtgga atgaaaaacg cgataccaag 900

cgcgctaagc tgctgcaata tgctgaacgc gtctggaaca tcactgaagg ttccgatgat 960

gagcgtattg acgccgcgat tgccgcaacc cgcaatttct ttgagcaatt aggcgtgccg 1020

acccacctct ccgactacgg tctggacggc agctccatcc cggctttgct gaaaaaactg 1080

gaagagcacg gcatgaccca actgggcgaa aatcatgaca ttacgttgga tgtcagccgc 1140

cgtatatacg aagccgcccg ctaa 1164

<210> 8

<211> 387

<212> PRT

<213> Unkown

<400> 8

Met Asn Asn Phe Asn Leu His Thr Pro Thr Arg Ile Leu Phe Gly Lys

1 5 10 15

Gly Ala Ile Ala Gly Leu Arg Glu Gln Ile Pro His Asp Ala Arg Val

20 25 30

Leu Ile Thr Tyr Gly Gly Gly Ser Val Lys Lys Thr Gly Val Leu Asp

35 40 45

Gln Val Leu Asp Ala Leu Lys Gly Met Asp Val Leu Glu Phe Gly Gly

50 55 60

Ile Glu Pro Asn Pro Ala Tyr Glu Thr Leu Met Asn Ala Val Lys Leu

65 70 75 80

Val Arg Glu Gln Lys Val Thr Phe Leu Leu Ala Val Gly Gly Gly Ser

85 90 95

Val Leu Asp Gly Thr Lys Phe Ile Ala Ala Ala Ala Asn Tyr Pro Glu

100 105 110

Asn Ile Asp Pro Trp His Ile Leu Gln Thr Gly Gly Lys Glu Ile Lys

115 120 125

Ser Ala Ile Pro Met Gly Cys Val Leu Thr Leu Pro Ala Thr Gly Ser

130 135 140

Glu Ser Asn Ala Gly Ala Val Ile Ser Arg Lys Thr Thr Gly Asp Lys

145 150 155 160

Gln Ala Phe His Ser Ala His Val Gln Pro Val Phe Ala Val Leu Asp

165 170 175

Pro Val Tyr Thr Tyr Thr Leu Pro Pro Arg Gln Val Ala Asn Gly Val

180 185 190

Val Asp Ala Phe Val His Thr Val Glu Gln Tyr Val Thr Lys Pro Val

195 200 205

Asp Ala Lys Ile Gln Asp Arg Phe Ala Glu Gly Ile Leu Leu Thr Leu

210 215 220

Ile Glu Asp Gly Pro Lys Ala Leu Lys Glu Pro Glu Asn Tyr Asp Val

225 230 235 240

Arg Ala Asn Val Met Trp Ala Ala Thr Gln Ala Leu Asn Gly Leu Ile

245 250 255

Gly Ala Gly Val Pro Gln Asp Trp Ala Thr His Met Leu Gly His Glu

260 265 270

Leu Thr Ala Met His Gly Leu Asp His Ala Gln Thr Leu Ala Ile Val

275 280 285

Leu Pro Ala Leu Trp Asn Glu Lys Arg Asp Thr Lys Arg Ala Lys Leu

290 295 300

Leu Gln Tyr Ala Glu Arg Val Trp Asn Ile Thr Glu Gly Ser Asp Asp

305 310 315 320

Glu Arg Ile Asp Ala Ala Ile Ala Ala Thr Arg Asn Phe Phe Glu Gln

325 330 335

Leu Gly Val Pro Thr His Leu Ser Asp Tyr Gly Leu Asp Gly Ser Ser

340 345 350

Ile Pro Ala Leu Leu Lys Lys Leu Glu Glu His Gly Met Thr Gln Leu

355 360 365

Gly Glu Asn His Asp Ile Thr Leu Asp Val Ser Arg Arg Ile Tyr Glu

370 375 380

Ala Ala Arg

385

<210> 9

<211> 828

<212> DNA

<213> Unkown

<400> 9

atggctaatc caaccgttat taagctacag gatggcaatg tcatgcccca gctgggactg 60

ggcgtctggc aagcaagtaa tgaggaagta atcaccgcca ttcaaaaagc gttagaagtg 120

ggttatcgct cgattgatac cgccgcggcc tacaagaacg aagaaggtgt cggcaaagcc 180

ctgaaaaatg cctcagtcaa cagagaagaa ctgttcatca ccactaagct gtggaacgac 240

gaccacaagc gcccccgcga agccctgctc gacagcctga aaaaactcca gcttgattat 300

atcgacctct acttaatgca ctggcccgtt cccgctatcg accattatgt cgaagcatgg 360

aaaggcatga tcgaattgca aaaagaggga ttaatcaaaa gcatcggcgt gtgcaacttc 420

cagatccatc acctgcaacg cctgattgat gaaactggcg tgacgcctgt gataaaccag 480

atcgaacttc atccgctgat gcaacaacgc cagctacacg cctggaacgc gacacacaaa 540

atccagaccg aatcctggag cccattagcg caaggaggga aaggcgtttt cgatcagaaa 600

gtcattcgcg atctggcaga taaatacggc aaaaccccgg cgcagattgt tatccgctgg 660

catctggata gcggcctggt ggtgatcccg aaatcggtca caccttcacg tattgccgaa 720

aactttgatg tctgggattt ccgtctcgac aaagacgaac tcggcgaaat tgcaaaactc 780

gatcagggca agcgtctcgg tcccgatcct gaccagttcg gcggctaa 828

<210> 10

<211> 275

<212> PRT

<213> Unkown

<400> 10

Met Ala Asn Pro Thr Val Ile Lys Leu Gln Asp Gly Asn Val Met Pro

1 5 10 15

Gln Leu Gly Leu Gly Val Trp Gln Ala Ser Asn Glu Glu Val Ile Thr

20 25 30

Ala Ile Gln Lys Ala Leu Glu Val Gly Tyr Arg Ser Ile Asp Thr Ala

35 40 45

Ala Ala Tyr Lys Asn Glu Glu Gly Val Gly Lys Ala Leu Lys Asn Ala

50 55 60

Ser Val Asn Arg Glu Glu Leu Phe Ile Thr Thr Lys Leu Trp Asn Asp

65 70 75 80

Asp His Lys Arg Pro Arg Glu Ala Leu Leu Asp Ser Leu Lys Lys Leu

85 90 95

Gln Leu Asp Tyr Ile Asp Leu Tyr Leu Met His Trp Pro Val Pro Ala

100 105 110

Ile Asp His Tyr Val Glu Ala Trp Lys Gly Met Ile Glu Leu Gln Lys

115 120 125

Glu Gly Leu Ile Lys Ser Ile Gly Val Cys Asn Phe Gln Ile His His

130 135 140

Leu Gln Arg Leu Ile Asp Glu Thr Gly Val Thr Pro Val Ile Asn Gln

145 150 155 160

Ile Glu Leu His Pro Leu Met Gln Gln Arg Gln Leu His Ala Trp Asn

165 170 175

Ala Thr His Lys Ile Gln Thr Glu Ser Trp Ser Pro Leu Ala Gln Gly

180 185 190

Gly Lys Gly Val Phe Asp Gln Lys Val Ile Arg Asp Leu Ala Asp Lys

195 200 205

Tyr Gly Lys Thr Pro Ala Gln Ile Val Ile Arg Trp His Leu Asp Ser

210 215 220

Gly Leu Val Val Ile Pro Lys Ser Val Thr Pro Ser Arg Ile Ala Glu

225 230 235 240

Asn Phe Asp Val Trp Asp Phe Arg Leu Asp Lys Asp Glu Leu Gly Glu

245 250 255

Ile Ala Lys Leu Asp Gln Gly Lys Arg Leu Gly Pro Asp Pro Asp Gln

260 265 270

Phe Gly Gly

275

<210> 11

<211> 1050

<212> DNA

<213> Unkown

<400> 11

atgaagatca aagctgttgg tgcatattcc gctaaacaac cacttgaacc gatggatatc 60

acccggcgtg aaccgggacc gaatgatgtc aaaatcgaaa tcgcttactg tggcgtttgc 120

cattccgatc tccaccaggt ccgttccgag tgggcgggga cggtttaccc ctgcgtgccg 180

ggtcatgaaa ttgtggggcg tgtggtagcc gttggtgatc aggtagaaaa atatgcgccg 240

ggcgatctgg tcggtgtcgg ctgcattgtc gacagttgta aacattgcga agagtgtgaa 300

gacgggttgg aaaactactg tgatcacatg accggcacct ataactcgcc gacgccggac 360

gaaccgggcc atactctggg cggctactca caacagatcg tcgttcatga gcgatatgtt 420

ctgcgtattc gtcacccgca agagcagctg gcggcggtgg ctcctttgtt gtgtgcaggg 480

atcaccacgt attcgccgct acgtcactgg caggccgggc cgggtaaaaa agtgggcgtg 540

gtcggcatcg gcggtctggg acatatgggg attaagctgg cccacgcgat gggggcacat 600

gtggtggcat ttaccacttc tgaggcaaaa cgcgaagcgg caaaagccct gggggccgat 660

gaagttgtta actcacgcaa tgccgatgag atggcggctc atctgaagag tttcgatttc 720

attttgaata cagtagctgc gccacataat ctcgacgatt ttaccacctt gctgaagcgt 780

gatggcacca tgacgctggt tggtgcgcct gcgacaccgc ataaatcgcc ggaagttttc 840

aacctgatca tgaaacgccg tgcgatagcc ggttctatga ttggcggcat tccagaaact 900

caggagatgc tcgatttttg cgccgaacat ggcatcgtgg ctgatataga gatgattcgg 960

gccgatcaaa ttaatgaagc ctatgagcga atgctgcgcg gtgatgtgaa atatcgtttt 1020

gttatcgata atcgcacact aacagactga 1050

<210> 12

<211> 349

<212> PRT

<213> Unkown

<400> 12

Met Lys Ile Lys Ala Val Gly Ala Tyr Ser Ala Lys Gln Pro Leu Glu

1 5 10 15

Pro Met Asp Ile Thr Arg Arg Glu Pro Gly Pro Asn Asp Val Lys Ile

20 25 30

Glu Ile Ala Tyr Cys Gly Val Cys His Ser Asp Leu His Gln Val Arg

35 40 45

Ser Glu Trp Ala Gly Thr Val Tyr Pro Cys Val Pro Gly His Glu Ile

50 55 60

Val Gly Arg Val Val Ala Val Gly Asp Gln Val Glu Lys Tyr Ala Pro

65 70 75 80

Gly Asp Leu Val Gly Val Gly Cys Ile Val Asp Ser Cys Lys His Cys

85 90 95

Glu Glu Cys Glu Asp Gly Leu Glu Asn Tyr Cys Asp His Met Thr Gly

100 105 110

Thr Tyr Asn Ser Pro Thr Pro Asp Glu Pro Gly His Thr Leu Gly Gly

115 120 125

Tyr Ser Gln Gln Ile Val Val His Glu Arg Tyr Val Leu Arg Ile Arg

130 135 140

His Pro Gln Glu Gln Leu Ala Ala Val Ala Pro Leu Leu Cys Ala Gly

145 150 155 160

Ile Thr Thr Tyr Ser Pro Leu Arg His Trp Gln Ala Gly Pro Gly Lys

165 170 175

Lys Val Gly Val Val Gly Ile Gly Gly Leu Gly His Met Gly Ile Lys

180 185 190

Leu Ala His Ala Met Gly Ala His Val Val Ala Phe Thr Thr Ser Glu

195 200 205

Ala Lys Arg Glu Ala Ala Lys Ala Leu Gly Ala Asp Glu Val Val Asn

210 215 220

Ser Arg Asn Ala Asp Glu Met Ala Ala His Leu Lys Ser Phe Asp Phe

225 230 235 240

Ile Leu Asn Thr Val Ala Ala Pro His Asn Leu Asp Asp Phe Thr Thr

245 250 255

Leu Leu Lys Arg Asp Gly Thr Met Thr Leu Val Gly Ala Pro Ala Thr

260 265 270

Pro His Lys Ser Pro Glu Val Phe Asn Leu Ile Met Lys Arg Arg Ala

275 280 285

Ile Ala Gly Ser Met Ile Gly Gly Ile Pro Glu Thr Gln Glu Met Leu

290 295 300

Asp Phe Cys Ala Glu His Gly Ile Val Ala Asp Ile Glu Met Ile Arg

305 310 315 320

Ala Asp Gln Ile Asn Glu Ala Tyr Glu Arg Met Leu Arg Gly Asp Val

325 330 335

Lys Tyr Arg Phe Val Ile Asp Asn Arg Thr Leu Thr Asp

340 345

<210> 13

<211> 1020

<212> DNA

<213> Unkown

<400> 13

atgtcgatga taaaaagcta tgccgcaaaa gaagcgggcg gcgaactgga agtttatgag 60

tacgatcccg gtgagctgag gccacaagat gttgaagtgc aggtggatta ctgcgggatc 120

tgccattccg atctgtcgat gatcgataac gaatggggat tttcacaata tccgctggtt 180

gccgggcatg aggtgattgg gcgcgtggtg gcactcggga gcgccgcgca ggataaaggt 240

ttgcaggtcg gtcagcgtgt cgggattggc tggacggcgc gtagctgtgg tcactgcgac 300

gcctgtatta gcggtaatca gatcaactgc gagcaaggtg cggtgccgac gattatgaat 360

cgcggtggct ttgccgagaa gttgcgtgcg gactggcaat gggtgattcc actgccagaa 420

aatattgata tcgagtccgc cgggccgctg ttgtgcggcg gtatcacggt ctttaaacca 480

ctgttgatgc accatatcac tgctaccagc cgcgttgggg taattggtat tggcgggctg 540

gggcatatcg ctataaaact tctgcacgca atgggatgcg aggtgacagc ctttagttct 600

aatccggcga aagagcagga agtgctggcg atgggtgccg ataaagtggt gaatagccgc 660

gatccgcagg cactgaaagc actggcgggg cagtttgatc tcattatcaa caccgtcaac 720

gtcagcctcg actggcagcc ctattttgag gcgctgacct atggcggtaa tttccatacg 780

gtcggtgcgg ttctcacgcc gctgtctgtt ccggccttta cgttaattgc gggcgatcgc 840

agcgtctctg gttctgctac cggcacgcct tatgagctgc gtaagctgat gcgttttgcc 900

gcccgcagca aggttgcgcc gaccaccgaa ctgttcccga tgtcgaaaat taacgacgcc 960

atccagcatg tgcgcgacgg taaggcgcgt taccgcgtgg tgttgaaagc cgatttttga 1020

<210> 14

<211> 339

<212> PRT

<213> Unkown

<400> 14

Met Ser Met Ile Lys Ser Tyr Ala Ala Lys Glu Ala Gly Gly Glu Leu

1 5 10 15

Glu Val Tyr Glu Tyr Asp Pro Gly Glu Leu Arg Pro Gln Asp Val Glu

20 25 30

Val Gln Val Asp Tyr Cys Gly Ile Cys His Ser Asp Leu Ser Met Ile

35 40 45

Asp Asn Glu Trp Gly Phe Ser Gln Tyr Pro Leu Val Ala Gly His Glu

50 55 60

Val Ile Gly Arg Val Val Ala Leu Gly Ser Ala Ala Gln Asp Lys Gly

65 70 75 80

Leu Gln Val Gly Gln Arg Val Gly Ile Gly Trp Thr Ala Arg Ser Cys

85 90 95

Gly His Cys Asp Ala Cys Ile Ser Gly Asn Gln Ile Asn Cys Glu Gln

100 105 110

Gly Ala Val Pro Thr Ile Met Asn Arg Gly Gly Phe Ala Glu Lys Leu

115 120 125

Arg Ala Asp Trp Gln Trp Val Ile Pro Leu Pro Glu Asn Ile Asp Ile

130 135 140

Glu Ser Ala Gly Pro Leu Leu Cys Gly Gly Ile Thr Val Phe Lys Pro

145 150 155 160

Leu Leu Met His His Ile Thr Ala Thr Ser Arg Val Gly Val Ile Gly

165 170 175

Ile Gly Gly Leu Gly His Ile Ala Ile Lys Leu Leu His Ala Met Gly

180 185 190

Cys Glu Val Thr Ala Phe Ser Ser Asn Pro Ala Lys Glu Gln Glu Val

195 200 205

Leu Ala Met Gly Ala Asp Lys Val Val Asn Ser Arg Asp Pro Gln Ala

210 215 220

Leu Lys Ala Leu Ala Gly Gln Phe Asp Leu Ile Ile Asn Thr Val Asn

225 230 235 240

Val Ser Leu Asp Trp Gln Pro Tyr Phe Glu Ala Leu Thr Tyr Gly Gly

245 250 255

Asn Phe His Thr Val Gly Ala Val Leu Thr Pro Leu Ser Val Pro Ala

260 265 270

Phe Thr Leu Ile Ala Gly Asp Arg Ser Val Ser Gly Ser Ala Thr Gly

275 280 285

Thr Pro Tyr Glu Leu Arg Lys Leu Met Arg Phe Ala Ala Arg Ser Lys

290 295 300

Val Ala Pro Thr Thr Glu Leu Phe Pro Met Ser Lys Ile Asn Asp Ala

305 310 315 320

Ile Gln His Val Arg Asp Gly Lys Ala Arg Tyr Arg Val Val Leu Lys

325 330 335

Ala Asp Phe

<210> 15

<211> 1053

<212> DNA

<213> Unkown

<400> 15

atgaattatc agaacgacga tttacgcatc aaagaaatca aagagttact tcctcctgtc 60

gcattgctgg aaaaattccc cgctactgaa aatgccgcga atacggttgc ccatgcccga 120

aaagcgatcc ataagatcct gaaaggtaat gatgatcgcc tgttggttgt gattggccca 180

tgctcaattc atgatcctgt cgcggcaaaa gagtatgcca ctcgcttgct ggcgctgcgt 240

gaagagctga aagatgagct ggaaatcgta atgcgcgtct attttgaaaa gccgcgtacc 300

acggtgggct ggaaagggct gattaacgat ccgcatatgg ataatagctt ccagatcaac 360

gacggtctgc gtatagcccg taaattgctg cttgatatta acgacagcgg tctgccagcg 420

gcaggtgagt ttctcaatat gatcacccca caatatctcg ctgacctgat gagctggggc 480

gcaattggcg cacgtaccac cgaatcgcag gtgcaccgcg aactggcatc agggctttct 540

tgtccggtcg gcttcaaaaa tggcaccgac ggtacgatta aagtggctat cgatgccatt 600

aatgccgccg gtgcgccgca ctgcttcctg tccgtaacga aatgggggca ttcggcgatt 660

gtgaatacca gcggtaacgg cgattgccat atcattctgc gcggcggtaa agagcctaac 720

tacagcgcga agcacgttgc tgaagtgaaa gaagggctga acaaagcagg cctgccagca 780

caggtgatga tcgatttcag ccatgctaac tcgtccaaac aattcaaaaa gcagatggat 840

gtttgtgctg acgtttgcca gcagattgcc ggtggcgaaa aggccattat tggcgtgatg 900

gtggaaagcc atctggtgga aggcaatcag agcctcgaga gcggggagcc gctggcctac 960

ggtaagagca tcaccgatgc ctgcatcggc tgggaagata ccgatgctct gttacgtcaa 1020

ctggcgaatg cagtaaaagc gcgtcgcggg taa 1053

<210> 16

<211> 350

<212> PRT

<213> Unkown

<400> 16

Met Asn Tyr Gln Asn Asp Asp Leu Arg Ile Lys Glu Ile Lys Glu Leu

1 5 10 15

Leu Pro Pro Val Ala Leu Leu Glu Lys Phe Pro Ala Thr Glu Asn Ala

20 25 30

Ala Asn Thr Val Ala His Ala Arg Lys Ala Ile His Lys Ile Leu Lys

35 40 45

Gly Asn Asp Asp Arg Leu Leu Val Val Ile Gly Pro Cys Ser Ile His

50 55 60

Asp Pro Val Ala Ala Lys Glu Tyr Ala Thr Arg Leu Leu Ala Leu Arg

65 70 75 80

Glu Glu Leu Lys Asp Glu Leu Glu Ile Val Met Arg Val Tyr Phe Glu

85 90 95

Lys Pro Arg Thr Thr Val Gly Trp Lys Gly Leu Ile Asn Asp Pro His

100 105 110

Met Asp Asn Ser Phe Gln Ile Asn Asp Gly Leu Arg Ile Ala Arg Lys

115 120 125

Leu Leu Leu Asp Ile Asn Asp Ser Gly Leu Pro Ala Ala Gly Glu Phe

130 135 140

Leu Asn Met Ile Thr Pro Gln Tyr Leu Ala Asp Leu Met Ser Trp Gly

145 150 155 160

Ala Ile Gly Ala Arg Thr Thr Glu Ser Gln Val His Arg Glu Leu Ala

165 170 175

Ser Gly Leu Ser Cys Pro Val Gly Phe Lys Asn Gly Thr Asp Gly Thr

180 185 190

Ile Lys Val Ala Ile Asp Ala Ile Asn Ala Ala Gly Ala Pro His Cys

195 200 205

Phe Leu Ser Val Thr Lys Trp Gly His Ser Ala Ile Val Asn Thr Ser

210 215 220

Gly Asn Gly Asp Cys His Ile Ile Leu Arg Gly Gly Lys Glu Pro Asn

225 230 235 240

Tyr Ser Ala Lys His Val Ala Glu Val Lys Glu Gly Leu Asn Lys Ala

245 250 255

Gly Leu Pro Ala Gln Val Met Ile Asp Phe Ser His Ala Asn Ser Ser

260 265 270

Lys Gln Phe Lys Lys Gln Met Asp Val Cys Ala Asp Val Cys Gln Gln

275 280 285

Ile Ala Gly Gly Glu Lys Ala Ile Ile Gly Val Met Val Glu Ser His

290 295 300

Leu Val Glu Gly Asn Gln Ser Leu Glu Ser Gly Glu Pro Leu Ala Tyr

305 310 315 320

Gly Lys Ser Ile Thr Asp Ala Cys Ile Gly Trp Glu Asp Thr Asp Ala

325 330 335

Leu Leu Arg Gln Leu Ala Asn Ala Val Lys Ala Arg Arg Gly

340 345 350

<210> 17

<211> 1122

<212> DNA

<213> Unkown

<400> 17

atggttgctg aattgaccgc attacgcgat caaattgatg aagtcgataa agcgctgctg 60

aatttattag cgaagcgtct ggaactggtt gctgaagtgg gcgaggtgaa aagccgcttt 120

ggactgccta tttatgttcc ggagcgcgag gcatctattt tggcctcgcg gcgcgcagag 180

gcggaagctc tgggtgtacc gccagatctg attgaggatg ttttgcgtcg ggtgatgcgt 240

gaatcttact ccagtgaaaa cgacaaagga tttaaaacac tttgtccgtc actgcgtccg 300

gtggttatcg tcggcggtgg cggtcagatg ggacgcctgt tcgagaagat gctgacacta 360

tcgggttatc aggtgcggat tctggagcaa catgactggg atcgagcggc tgatattgtt 420

gccgatgccg gaatggtgat tgttagtgtg ccaatccacg ttactgagca agttatcggc 480

aaattaccgc ctttaccgaa agattgtatt ctggttgatc tggcatcagt gaaaaatgga 540

ccattacagg ccatgctggc ggcgcacgat ggcccggtac tggggttaca cccgatgttc 600

ggcccggaca gcggtagcct ggcaaagcaa gttgtggtct ggtgtgatgg acgtaagccg 660

gaagcatacc aatggtttct ggagcaaatt caggtctggg gcgctcggct gcatcgtatt 720

agcgctgtcg agcacgatca gaatatggcg tttattcagg ctctgcgcca ctttgctact 780

tttgcttatg ggctgcatct ggcagaagaa aatgttcagc ttgagcaact tctggcgctc 840

tcttcgccga tttaccgcct tgagctggcg atggtcgggc gactgtttgc tcaggatccg 900

cagctttatg ccgacattat tatgtcgtca gagcgtaatc tggcgttaat caaacgttac 960

tataagcgtt tcggcgaggc gattgagttg ctggagcagg gcgataagca ggcgtttatt 1020

gacagtttcc gcaaggtgga gcactggttc ggcgattacg ttcagcgttt tcagagtgaa 1080

agccgcgtgt tattgcgtca ggcgaatgac aatcgccagt aa 1122

<210> 18

<211> 373

<212> PRT

<213> Unkown

<400> 18

Met Val Ala Glu Leu Thr Ala Leu Arg Asp Gln Ile Asp Glu Val Asp

1 5 10 15

Lys Ala Leu Leu Asn Leu Leu Ala Lys Arg Leu Glu Leu Val Ala Glu

20 25 30

Val Gly Glu Val Lys Ser Arg Phe Gly Leu Pro Ile Tyr Val Pro Glu

35 40 45

Arg Glu Ala Ser Ile Leu Ala Ser Arg Arg Ala Glu Ala Glu Ala Leu

50 55 60

Gly Val Pro Pro Asp Leu Ile Glu Asp Val Leu Arg Arg Val Met Arg

65 70 75 80

Glu Ser Tyr Ser Ser Glu Asn Asp Lys Gly Phe Lys Thr Leu Cys Pro

85 90 95

Ser Leu Arg Pro Val Val Ile Val Gly Gly Gly Gly Gln Met Gly Arg

100 105 110

Leu Phe Glu Lys Met Leu Thr Leu Ser Gly Tyr Gln Val Arg Ile Leu

115 120 125

Glu Gln His Asp Trp Asp Arg Ala Ala Asp Ile Val Ala Asp Ala Gly

130 135 140

Met Val Ile Val Ser Val Pro Ile His Val Thr Glu Gln Val Ile Gly

145 150 155 160

Lys Leu Pro Pro Leu Pro Lys Asp Cys Ile Leu Val Asp Leu Ala Ser

165 170 175

Val Lys Asn Gly Pro Leu Gln Ala Met Leu Ala Ala His Asp Gly Pro

180 185 190

Val Leu Gly Leu His Pro Met Phe Gly Pro Asp Ser Gly Ser Leu Ala

195 200 205

Lys Gln Val Val Val Trp Cys Asp Gly Arg Lys Pro Glu Ala Tyr Gln

210 215 220

Trp Phe Leu Glu Gln Ile Gln Val Trp Gly Ala Arg Leu His Arg Ile

225 230 235 240

Ser Ala Val Glu His Asp Gln Asn Met Ala Phe Ile Gln Ala Leu Arg

245 250 255

His Phe Ala Thr Phe Ala Tyr Gly Leu His Leu Ala Glu Glu Asn Val

260 265 270

Gln Leu Glu Gln Leu Leu Ala Leu Ser Ser Pro Ile Tyr Arg Leu Glu

275 280 285

Leu Ala Met Val Gly Arg Leu Phe Ala Gln Asp Pro Gln Leu Tyr Ala

290 295 300

Asp Ile Ile Met Ser Ser Glu Arg Asn Leu Ala Leu Ile Lys Arg Tyr

305 310 315 320

Tyr Lys Arg Phe Gly Glu Ala Ile Glu Leu Leu Glu Gln Gly Asp Lys

325 330 335

Gln Ala Phe Ile Asp Ser Phe Arg Lys Val Glu His Trp Phe Gly Asp

340 345 350

Tyr Val Gln Arg Phe Gln Ser Glu Ser Arg Val Leu Leu Arg Gln Ala

355 360 365

Asn Asp Asn Arg Gln

370

<210> 19

<211> 1908

<212> DNA

<213> Unkown

<400> 19

atggcacctg ttacaattga aaagttcgta aatcaagaag aacgacacct tgtttccaac 60

cgatcagcaa caattccgtt tggtgaatac atatttaaaa gattgttgtc catcgatacg 120

aaatcagttt tcggtgttcc tggtgacttc aacttatctc tattagaata tctctattca 180

cctagtgttg aatcagctgg cctaagatgg gtcggcacgt gtaatgaact gaacgccgct 240

tatgcggccg acggatattc ccgttactct aataagattg gctgtttaat aaccacgtat 300

ggcgttggtg aattaagcgc cttgaacggt atagccggtt cgttcgctga aaatgtcaaa 360

gttttgcaca ttgttggtgt ggccaagtcc atagattcgc gttcaagtaa ctttagtgat 420

cggaacctac atcatttggt cccacagcta catgattcaa attttaaagg gccaaatcat 480

aaagtatatc atgatatggt aaaagataga gtcgcttgct cggtagccta cttggaggat 540

attgaaactg catgtgacca agtcgataat gttatccgcg atatttacaa gtattctaaa 600

cctggttata tttttgttcc tgcagatttt gcggatatgt ctgttacatg tgataatttg 660

gttaatgttc cacgtatatc tcaacaagat tgtatagtat acccttctga aaaccaattg 720

tctgacataa tcaacaagat tactagttgg atatattcca gtaaaacacc tgcgatcctt 780

ggagacgtac tgactgatag gtatggtgtg agtaactttt tgaacaagct tatctgcaaa 840

actgggattt ggaatttttc cactgttatg ggaaaatctg taattgatga gtcaaaccca 900

acttatatgg gtcaatataa tggtaaagaa ggtttaaaac aagtctatga acattttgaa 960

ctgtgcgact tggtcttgca ttttggagtc gacatcaatg aaattaataa tgggcattat 1020

acttttactt ataaaccaaa tgctaaaatc attcaatttc atccgaatta tattcgcctt 1080

gtggacacta ggcagggcaa tgagcaaatg ttcaaaggaa tcaattttgc ccctatttta 1140

aaagaactat acaagcgcat tgacgtttct aaactttctt tgcaatatga ttcaaatgta 1200

actcaatata cgaacgaaac aatgcggtta gaagatccta ccaatggaca atcaagcatt 1260

attacacaag ttcacttaca aaagacgatg cctaaatttt tgaaccctgg tgatgttgtc 1320

gtttgtgaaa caggctcttt tcaattctct gttcgtgatt tcgcgtttcc ttcgcaatta 1380

aaatatatat cgcaaggatt tttcctttcc attggcatgg cccttcctgc cgccctaggt 1440

gttggaattg ccatgcaaga ccactcaaac gctcacatca atggtggcaa cgtaaaagag 1500

gactataagc caagattaat tttgtttgaa ggtgacggtg cagcacagat gacaatccaa 1560

gaactgagca ccattctgaa gtgcaatatt ccactagaag ttatcatttg gaacaataac 1620

ggctacacta ttgaaagagc catcatgggc cctaccaggt cgtataacga cgttatgtct 1680

tggaaatgga ccaaactatt tgaagcattc ggagacttcg acggaaagta tactaatagc 1740

actctcattc aatgtccctc taaattagca ctgaaattgg aggagcttaa gaattcaaac 1800

aaaagaagcg ggatagaact tttagaagtc aaattaggcg aattggattt ccccgaacag 1860

ctaaagtgca tggttgaagc agcggcactt aaaagaaata aaaaatag 1908

<210> 20

<211> 635

<212> PRT

<213> Unkown

<400> 20

Met Ala Pro Val Thr Ile Glu Lys Phe Val Asn Gln Glu Glu Arg His

1 5 10 15

Leu Val Ser Asn Arg Ser Ala Thr Ile Pro Phe Gly Glu Tyr Ile Phe

20 25 30

Lys Arg Leu Leu Ser Ile Asp Thr Lys Ser Val Phe Gly Val Pro Gly

35 40 45

Asp Phe Asn Leu Ser Leu Leu Glu Tyr Leu Tyr Ser Pro Ser Val Glu

50 55 60

Ser Ala Gly Leu Arg Trp Val Gly Thr Cys Asn Glu Leu Asn Ala Ala

65 70 75 80

Tyr Ala Ala Asp Gly Tyr Ser Arg Tyr Ser Asn Lys Ile Gly Cys Leu

85 90 95

Ile Thr Thr Tyr Gly Val Gly Glu Leu Ser Ala Leu Asn Gly Ile Ala

100 105 110

Gly Ser Phe Ala Glu Asn Val Lys Val Leu His Ile Val Gly Val Ala

115 120 125

Lys Ser Ile Asp Ser Arg Ser Ser Asn Phe Ser Asp Arg Asn Leu His

130 135 140

His Leu Val Pro Gln Leu His Asp Ser Asn Phe Lys Gly Pro Asn His

145 150 155 160

Lys Val Tyr His Asp Met Val Lys Asp Arg Val Ala Cys Ser Val Ala

165 170 175

Tyr Leu Glu Asp Ile Glu Thr Ala Cys Asp Gln Val Asp Asn Val Ile

180 185 190

Arg Asp Ile Tyr Lys Tyr Ser Lys Pro Gly Tyr Ile Phe Val Pro Ala

195 200 205

Asp Phe Ala Asp Met Ser Val Thr Cys Asp Asn Leu Val Asn Val Pro

210 215 220

Arg Ile Ser Gln Gln Asp Cys Ile Val Tyr Pro Ser Glu Asn Gln Leu

225 230 235 240

Ser Asp Ile Ile Asn Lys Ile Thr Ser Trp Ile Tyr Ser Ser Lys Thr

245 250 255

Pro Ala Ile Leu Gly Asp Val Leu Thr Asp Arg Tyr Gly Val Ser Asn

260 265 270

Phe Leu Asn Lys Leu Ile Cys Lys Thr Gly Ile Trp Asn Phe Ser Thr

275 280 285

Val Met Gly Lys Ser Val Ile Asp Glu Ser Asn Pro Thr Tyr Met Gly

290 295 300

Gln Tyr Asn Gly Lys Glu Gly Leu Lys Gln Val Tyr Glu His Phe Glu

305 310 315 320

Leu Cys Asp Leu Val Leu His Phe Gly Val Asp Ile Asn Glu Ile Asn

325 330 335

Asn Gly His Tyr Thr Phe Thr Tyr Lys Pro Asn Ala Lys Ile Ile Gln

340 345 350

Phe His Pro Asn Tyr Ile Arg Leu Val Asp Thr Arg Gln Gly Asn Glu

355 360 365

Gln Met Phe Lys Gly Ile Asn Phe Ala Pro Ile Leu Lys Glu Leu Tyr

370 375 380

Lys Arg Ile Asp Val Ser Lys Leu Ser Leu Gln Tyr Asp Ser Asn Val

385 390 395 400

Thr Gln Tyr Thr Asn Glu Thr Met Arg Leu Glu Asp Pro Thr Asn Gly

405 410 415

Gln Ser Ser Ile Ile Thr Gln Val His Leu Gln Lys Thr Met Pro Lys

420 425 430

Phe Leu Asn Pro Gly Asp Val Val Val Cys Glu Thr Gly Ser Phe Gln

435 440 445

Phe Ser Val Arg Asp Phe Ala Phe Pro Ser Gln Leu Lys Tyr Ile Ser

450 455 460

Gln Gly Phe Phe Leu Ser Ile Gly Met Ala Leu Pro Ala Ala Leu Gly

465 470 475 480

Val Gly Ile Ala Met Gln Asp His Ser Asn Ala His Ile Asn Gly Gly

485 490 495

Asn Val Lys Glu Asp Tyr Lys Pro Arg Leu Ile Leu Phe Glu Gly Asp

500 505 510

Gly Ala Ala Gln Met Thr Ile Gln Glu Leu Ser Thr Ile Leu Lys Cys

515 520 525

Asn Ile Pro Leu Glu Val Ile Ile Trp Asn Asn Asn Gly Tyr Thr Ile

530 535 540

Glu Arg Ala Ile Met Gly Pro Thr Arg Ser Tyr Asn Asp Val Met Ser

545 550 555 560

Trp Lys Trp Thr Lys Leu Phe Glu Ala Phe Gly Asp Phe Asp Gly Lys

565 570 575

Tyr Thr Asn Ser Thr Leu Ile Gln Cys Pro Ser Lys Leu Ala Leu Lys

580 585 590

Leu Glu Glu Leu Lys Asn Ser Asn Lys Arg Ser Gly Ile Glu Leu Leu

595 600 605

Glu Val Lys Leu Gly Glu Leu Asp Phe Pro Glu Gln Leu Lys Cys Met

610 615 620

Val Glu Ala Ala Ala Leu Lys Arg Asn Lys Lys

625 630 635

<210> 21

<211> 1419

<212> DNA

<213> Unkown

<400> 21

atgacccccg aacagttccg tcagtatggc caccagctga tcgatttaat cgcagattat 60

cgccagaccg ttggtgaacg tccggttatg gcacaagttg aaccgggcta tctgaaagca 120

gctttaccgg caacagcacc tcaacaaggt gaacctttcg ccgccatttt agatgatgtg 180

aacaatctgg tgatgccggg tctgagccac tggcagcatc cggacttcta tggctacttt 240

ccgagcaatg gtactttaag cagtgtgctg ggcgactttc tgagtaccgg tctgggtgtt 300

ctgggtttaa gttggcagag cagcccggca ctgagcgaac tggaggaaac aacactggac 360

tggctgcgtc agctgctggg tctgagcggt cagtggagcg gtgtgatcca agatacagcc 420

agcaccagta ctttagtggc tttaattagc gcacgtgaac gcgccaccga ttacgcactg 480

gttcgtggtg gtttacaagc tgaaccgaaa ccgctgatcg tgtacgtgag cgcacatgcc 540

catagcagcg tggataaagc cgcactgctg gccggttttg gccgcgataa tattcgttta 600

attccgaccg acgaacgtta cgcactgcgt ccggaagctt tacaagctgc aattgagcaa 660

gatctggccg ccggcaatca gccgtgcgca gttgttgcaa ccaccggtac caccaccaca 720

accgcattag atccgctgcg tcccgttggc gaaatcgcac aagctaatgg tttatggctg 780

cacgtggaca gcgcaatggc tggtagcgcc atgattctgc cggaatgccg ttggatgtgg 840

gacggtatcg agttagcaga cagcgttgtg gtgaacgcac acaaatggct gggtgtggcc 900

ttcgactgca gcatctatta cgtgcgcgat ccgcagcact taatccgcgt gatgagcacc 960

aatccgagct atctgcagag cgcagtggac ggtgaggtga aaaatttacg tgattggggc 1020

attccgctgg gccgtcgttt ccgtgcttta aaactgtggt tcatgctgcg cagtgaaggc 1080

gtggatgcac tgcaagctcg tctgcgtcgt gatctggata atgcacagtg gctggctggt 1140

caagttgaag cagcagcaga atgggaggtg ctggccccgg ttcaactgca gactttatgc 1200

attcgccacc gtcccgctgg tctggaaggc gaggcactgg atgcccatac caaaggttgg 1260

gccgaacgtt taaatgcaag cggtgccgca tacgtgaccc cggcaacttt agatggtcgc 1320

tggatggtgc gtgttagcat tggtgcttta ccgaccgaac gtggtgatgt tcagcgtctg 1380

tgggcacgtt tacaagacgt gattaaaggc ggcagctaa 1419

<210> 22

<211> 472

<212> PRT

<213> Unkown

<400> 22

Met Thr Pro Glu Gln Phe Arg Gln Tyr Gly His Gln Leu Ile Asp Leu

1 5 10 15

Ile Ala Asp Tyr Arg Gln Thr Val Gly Glu Arg Pro Val Met Ala Gln

20 25 30

Val Glu Pro Gly Tyr Leu Lys Ala Ala Leu Pro Ala Thr Ala Pro Gln

35 40 45

Gln Gly Glu Pro Phe Ala Ala Ile Leu Asp Asp Val Asn Asn Leu Val

50 55 60

Met Pro Gly Leu Ser His Trp Gln His Pro Asp Phe Tyr Gly Tyr Phe

65 70 75 80

Pro Ser Asn Gly Thr Leu Ser Ser Val Leu Gly Asp Phe Leu Ser Thr

85 90 95

Gly Leu Gly Val Leu Gly Leu Ser Trp Gln Ser Ser Pro Ala Leu Ser

100 105 110

Glu Leu Glu Glu Thr Thr Leu Asp Trp Leu Arg Gln Leu Leu Gly Leu

115 120 125

Ser Gly Gln Trp Ser Gly Val Ile Gln Asp Thr Ala Ser Thr Ser Thr

130 135 140

Leu Val Ala Leu Ile Ser Ala Arg Glu Arg Ala Thr Asp Tyr Ala Leu

145 150 155 160

Val Arg Gly Gly Leu Gln Ala Glu Pro Lys Pro Leu Ile Val Tyr Val

165 170 175

Ser Ala His Ala His Ser Ser Val Asp Lys Ala Ala Leu Leu Ala Gly

180 185 190

Phe Gly Arg Asp Asn Ile Arg Leu Ile Pro Thr Asp Glu Arg Tyr Ala

195 200 205

Leu Arg Pro Glu Ala Leu Gln Ala Ala Ile Glu Gln Asp Leu Ala Ala

210 215 220

Gly Asn Gln Pro Cys Ala Val Val Ala Thr Thr Gly Thr Thr Thr Thr

225 230 235 240

Thr Ala Leu Asp Pro Leu Arg Pro Val Gly Glu Ile Ala Gln Ala Asn

245 250 255

Gly Leu Trp Leu His Val Asp Ser Ala Met Ala Gly Ser Ala Met Ile

260 265 270

Leu Pro Glu Cys Arg Trp Met Trp Asp Gly Ile Glu Leu Ala Asp Ser

275 280 285

Val Val Val Asn Ala His Lys Trp Leu Gly Val Ala Phe Asp Cys Ser

290 295 300

Ile Tyr Tyr Val Arg Asp Pro Gln His Leu Ile Arg Val Met Ser Thr

305 310 315 320

Asn Pro Ser Tyr Leu Gln Ser Ala Val Asp Gly Glu Val Lys Asn Leu

325 330 335

Arg Asp Trp Gly Ile Pro Leu Gly Arg Arg Phe Arg Ala Leu Lys Leu

340 345 350

Trp Phe Met Leu Arg Ser Glu Gly Val Asp Ala Leu Gln Ala Arg Leu

355 360 365

Arg Arg Asp Leu Asp Asn Ala Gln Trp Leu Ala Gly Gln Val Glu Ala

370 375 380

Ala Ala Glu Trp Glu Val Leu Ala Pro Val Gln Leu Gln Thr Leu Cys

385 390 395 400

Ile Arg His Arg Pro Ala Gly Leu Glu Gly Glu Ala Leu Asp Ala His

405 410 415

Thr Lys Gly Trp Ala Glu Arg Leu Asn Ala Ser Gly Ala Ala Tyr Val

420 425 430

Thr Pro Ala Thr Leu Asp Gly Arg Trp Met Val Arg Val Ser Ile Gly

435 440 445

Ala Leu Pro Thr Glu Arg Gly Asp Val Gln Arg Leu Trp Ala Arg Leu

450 455 460

Gln Asp Val Ile Lys Gly Gly Ser

465 470

<210> 23

<211> 609

<212> DNA

<213> Unkown

<400> 23

atgcgtaaag tgatcaaata tgatatggag gtggccgtga gcgccgatag cgtttgggca 60

gtgtatagta gcccggatat cccgcgtctg ctgcgcgatg ttctgctgcc gggtgtgttc 120

gagaagctgg acgtgatcga aggcaatggt ggtgttggca ccgttctgga cattgtgttt 180

ccgccgggcg cagttccgcg cagctataaa gaaaaatttg tgaacattga tcgcgaaaag 240

cgtttaaaag aagttatcat gatcgaaggc ggttatttag atatgggctg caccttctat 300

ctggatcgca ttcacgtggt ggagaaaacc aagagcagct gcgtgatcga gagcagcatt 360

gtgtacgacg tgaaagagga gtgtgccgat gccatgagca agctgattac caccgaaccg 420

ctgaaaagca tggccgaagt gattagcaat tatgtgattc agaaagagct gttcagcgcc 480

cgcaacattt taagcaagca gagcgtggtg aaaaaggaga tccgctatga tttagaggtg 540

ccgattagcg tggacagcat ttggagcgtg tacagctgcc cggatattcc tcgtttactg 600

ggcagctaa 609

<210> 24

<211> 202

<212> PRT

<213> Unkown

<400> 24

Met Arg Lys Val Ile Lys Tyr Asp Met Glu Val Ala Val Ser Ala Asp

1 5 10 15

Ser Val Trp Ala Val Tyr Ser Ser Pro Asp Ile Pro Arg Leu Leu Arg

20 25 30

Asp Val Leu Leu Pro Gly Val Phe Glu Lys Leu Asp Val Ile Glu Gly

35 40 45

Asn Gly Gly Val Gly Thr Val Leu Asp Ile Val Phe Pro Pro Gly Ala

50 55 60

Val Pro Arg Ser Tyr Lys Glu Lys Phe Val Asn Ile Asp Arg Glu Lys

65 70 75 80

Arg Leu Lys Glu Val Ile Met Ile Glu Gly Gly Tyr Leu Asp Met Gly

85 90 95

Cys Thr Phe Tyr Leu Asp Arg Ile His Val Val Glu Lys Thr Lys Ser

100 105 110

Ser Cys Val Ile Glu Ser Ser Ile Val Tyr Asp Val Lys Glu Glu Cys

115 120 125

Ala Asp Ala Met Ser Lys Leu Ile Thr Thr Glu Pro Leu Lys Ser Met

130 135 140

Ala Glu Val Ile Ser Asn Tyr Val Ile Gln Lys Glu Leu Phe Ser Ala

145 150 155 160

Arg Asn Ile Leu Ser Lys Gln Ser Val Val Lys Lys Glu Ile Arg Tyr

165 170 175

Asp Leu Glu Val Pro Ile Ser Val Asp Ser Ile Trp Ser Val Tyr Ser

180 185 190

Cys Pro Asp Ile Pro Arg Leu Leu Gly Ser

195 200

<210> 25

<211> 1041

<212> DNA

<213> Unkown

<400> 25

atggagaccg ttagcaaaat tgaccaacag aatcaagcta aaatctggaa gcagatctat 60

ggcttcgccg aatctttagt tctgaaatgt gccgtgcagc tggaaatcgc cgagacactg 120

cacaacaacg tgaaaccgat gtctttaagc gaactggcca gcaagctgcc ggttgcacag 180

ccggttaacg aagaccgttt attccgcatc atgcgctatt tagtgcatat ggaactgttc 240

aaaattgatg ccaccaccca gaaatactct ttagccccgc cggccaaata tttactgcgc 300

ggctgggaga aaagcatggt ggatagcatt ctgtgcatca acgataagga ctttctggcc 360

ccttggcacc atctgggtga tggtctgacc ggtaattgcg atgcattcga gaaagcttta 420

ggcaagagca tttgggtgta tatgagcgaa aacccggaga aaaaccagct gttcaacgcc 480

gcaatggcat gcgatacccg tttagtgaca agcgcactgg ccaacgagtg caagagcatc 540

tttagcgacg gcattagcac tttagttgat gttggcggcg gtaccggtac cgcagtgaag 600

gcaatcagca aagcattccc ggacattaaa tgcaccattt atgatttacc gcatgtgatc 660

gccgatagcc cggagattcc gaacatcacc aagatcagtg gcgacatgtt caaaagtatc 720

ccgagcgccg acgccatctt tatgaagtgc attttacacg actggaatga cgacgaatgc 780

attcagattt taaaacgctg caaagaagct ttaccgaaag ttggcaaggt gatcatcgtg 840

gacgtggtta tcgacatgga cagcacccat ccgtatgcca aaatccgttt aactttagat 900

ctggacatga tgctgaacac cggcggcaaa gaacgcacca aagaagaatg gaaaacttta 960

ttcgatgcag ccggttttgc cagccacaaa gtgacacaga ttagcgccgt gcagagcgtg 1020

attgaagcat atccgtacta a 1041

<210> 26

<211> 346

<212> PRT

<213> Unkown

<400> 26

Met Glu Thr Val Ser Lys Ile Asp Gln Gln Asn Gln Ala Lys Ile Trp

1 5 10 15

Lys Gln Ile Tyr Gly Phe Ala Glu Ser Leu Val Leu Lys Cys Ala Val

20 25 30

Gln Leu Glu Ile Ala Glu Thr Leu His Asn Asn Val Lys Pro Met Ser

35 40 45

Leu Ser Glu Leu Ala Ser Lys Leu Pro Val Ala Gln Pro Val Asn Glu

50 55 60

Asp Arg Leu Phe Arg Ile Met Arg Tyr Leu Val His Met Glu Leu Phe

65 70 75 80

Lys Ile Asp Ala Thr Thr Gln Lys Tyr Ser Leu Ala Pro Pro Ala Lys

85 90 95

Tyr Leu Leu Arg Gly Trp Glu Lys Ser Met Val Asp Ser Ile Leu Cys

100 105 110

Ile Asn Asp Lys Asp Phe Leu Ala Pro Trp His His Leu Gly Asp Gly

115 120 125

Leu Thr Gly Asn Cys Asp Ala Phe Glu Lys Ala Leu Gly Lys Ser Ile

130 135 140

Trp Val Tyr Met Ser Glu Asn Pro Glu Lys Asn Gln Leu Phe Asn Ala

145 150 155 160

Ala Met Ala Cys Asp Thr Arg Leu Val Thr Ser Ala Leu Ala Asn Glu

165 170 175

Cys Lys Ser Ile Phe Ser Asp Gly Ile Ser Thr Leu Val Asp Val Gly

180 185 190

Gly Gly Thr Gly Thr Ala Val Lys Ala Ile Ser Lys Ala Phe Pro Asp

195 200 205

Ile Lys Cys Thr Ile Tyr Asp Leu Pro His Val Ile Ala Asp Ser Pro

210 215 220

Glu Ile Pro Asn Ile Thr Lys Ile Ser Gly Asp Met Phe Lys Ser Ile

225 230 235 240

Pro Ser Ala Asp Ala Ile Phe Met Lys Cys Ile Leu His Asp Trp Asn

245 250 255

Asp Asp Glu Cys Ile Gln Ile Leu Lys Arg Cys Lys Glu Ala Leu Pro

260 265 270

Lys Val Gly Lys Val Ile Ile Val Asp Val Val Ile Asp Met Asp Ser

275 280 285

Thr His Pro Tyr Ala Lys Ile Arg Leu Thr Leu Asp Leu Asp Met Met

290 295 300

Leu Asn Thr Gly Gly Lys Glu Arg Thr Lys Glu Glu Trp Lys Thr Leu

305 310 315 320

Phe Asp Ala Ala Gly Phe Ala Ser His Lys Val Thr Gln Ile Ser Ala

325 330 335

Val Gln Ser Val Ile Glu Ala Tyr Pro Tyr

340 345

<210> 27

<211> 1053

<212> DNA

<213> Unkown

<400> 27

atggccttcc atggcaagga cgacgtgctg gatattaagg cccaagctca tgtgtggaag 60

atcatttacg gcttcgctga ttctttagtg ctgcgttgcg ccgtggagtt aggcatcgtg 120

gatatcattg ataacaataa ccaaccgatg gctttagccg atctggccag caaactgccg 180

gttagcgacg tgaactgcga taatctgtac cgcattctgc gctatttagt taagatggag 240

attttacgcg tggagaaaag cgatgacggc cagaaaaagt atgctttaga accgattgcc 300

actttactga gtcgcaatgc caaacgtagc atggtgccga tgattttagg catgacccag 360

aaggatttca tgaccccgtg gcacagcatg aaagatggtt taagcgataa tggcaccgcc 420

tttgagaagg caatgggcat gaccatctgg gaatatttag aaggtcaccc ggatcagagc 480

cagctgttca atgagggcat ggctggtgaa acccgtttac tgaccagcag tctgatcagc 540

ggtagccgcg acatgtttca aggtatcgat tctctggtgg atgttggtgg tggcaatggt 600

accaccgtta aggccattag cgacgccttc ccgcacatca aatgcacact gttcgattta 660

cctcacgtga tcgccaacag ctacgatctg ccgaacattg aacgcatcgg tggcgatatg 720

tttaaaagcg tgccgagcgc ccaagctatc attttaaagc tgattttaca tgactggaac 780

gatgaggaca gcatcaagat tttaaagcag tgccgcaacg ccgtgccgaa agatggcggc 840

aaagtgatca tcgtggatgt ggctttagac gaggaaagcg atcatgagct gagtagcacc 900

cgtctgattt tagacatcga tatgctggtg aataccggcg gtaaagagcg taccaaggaa 960

gtttgggaaa aaatcgtgaa gagcgccggc tttagcggct gcaaaattcg ccacattgcc 1020

gccatccaga gcgtgattga ggtgtttcct taa 1053

<210> 28

<211> 350

<212> PRT

<213> Unkown

<400> 28

Met Ala Phe His Gly Lys Asp Asp Val Leu Asp Ile Lys Ala Gln Ala

1 5 10 15

His Val Trp Lys Ile Ile Tyr Gly Phe Ala Asp Ser Leu Val Leu Arg

20 25 30

Cys Ala Val Glu Leu Gly Ile Val Asp Ile Ile Asp Asn Asn Asn Gln

35 40 45

Pro Met Ala Leu Ala Asp Leu Ala Ser Lys Leu Pro Val Ser Asp Val

50 55 60

Asn Cys Asp Asn Leu Tyr Arg Ile Leu Arg Tyr Leu Val Lys Met Glu

65 70 75 80

Ile Leu Arg Val Glu Lys Ser Asp Asp Gly Gln Lys Lys Tyr Ala Leu

85 90 95

Glu Pro Ile Ala Thr Leu Leu Ser Arg Asn Ala Lys Arg Ser Met Val

100 105 110

Pro Met Ile Leu Gly Met Thr Gln Lys Asp Phe Met Thr Pro Trp His

115 120 125

Ser Met Lys Asp Gly Leu Ser Asp Asn Gly Thr Ala Phe Glu Lys Ala

130 135 140

Met Gly Met Thr Ile Trp Glu Tyr Leu Glu Gly His Pro Asp Gln Ser

145 150 155 160

Gln Leu Phe Asn Glu Gly Met Ala Gly Glu Thr Arg Leu Leu Thr Ser

165 170 175

Ser Leu Ile Ser Gly Ser Arg Asp Met Phe Gln Gly Ile Asp Ser Leu

180 185 190

Val Asp Val Gly Gly Gly Asn Gly Thr Thr Val Lys Ala Ile Ser Asp

195 200 205

Ala Phe Pro His Ile Lys Cys Thr Leu Phe Asp Leu Pro His Val Ile

210 215 220

Ala Asn Ser Tyr Asp Leu Pro Asn Ile Glu Arg Ile Gly Gly Asp Met

225 230 235 240

Phe Lys Ser Val Pro Ser Ala Gln Ala Ile Ile Leu Lys Leu Ile Leu

245 250 255

His Asp Trp Asn Asp Glu Asp Ser Ile Lys Ile Leu Lys Gln Cys Arg

260 265 270

Asn Ala Val Pro Lys Asp Gly Gly Lys Val Ile Ile Val Asp Val Ala

275 280 285

Leu Asp Glu Glu Ser Asp His Glu Leu Ser Ser Thr Arg Leu Ile Leu

290 295 300

Asp Ile Asp Met Leu Val Asn Thr Gly Gly Lys Glu Arg Thr Lys Glu

305 310 315 320

Val Trp Glu Lys Ile Val Lys Ser Ala Gly Phe Ser Gly Cys Lys Ile

325 330 335

Arg His Ile Ala Ala Ile Gln Ser Val Ile Glu Val Phe Pro

340 345 350

<210> 29

<211> 1056

<212> DNA

<213> Unkown

<400> 29

atgcagttaa aagaaaaaga ggagctgctg cgcaacatgg aactgggtct gatcccggat 60

caagaaattc gccagcgtat ccgcatcgag ctggaaaaac gtttacagtg gggctacaaa 120

gagacccatg aggaacagct gagtcagctg ctggatttag tgcactcttt aaagggcatg 180

aaaatggcca ccgaaatgga gaatctggat ttaaagctgt atgaagcccc gatggagttt 240

ttaaagatcc agcacggtag caacatgaag cagagcgctg gttactatac cgacgaaagc 300

accactttag atgaagccga gattgccatg ctggatctgt acatggaacg cgcacagatc 360

aaggatggcc agagcgtttt agatttaggc tgcggcttag gcgccgttgc actgtttggc 420

gccaataaat ttaagaagtg ccagtttacc ggtgttacca gcagtgtgga gcagaaagac 480

tatattgaag gcaaatgcaa agaactgaaa ctgaccaacg tgaaagtgct gctggcagac 540

atcaccacct atgaaaccga ggagcgcttt gatcgcatct ttgccgtgga gctgatcgag 600

catatgaaaa actatcagct gttactgaag aaaattagcg agtggatgaa ggatgatggt 660

ttactgtttg tggaacacgt gtgccacaag actttagcct atcattacga accggtggac 720

gcagaggatt ggtacaccaa ctatattttc ccggccggca cactgacttt aagcagtgcc 780

agcatgctgc tgtatttcca agatgacgtt agcgtggtta accagtggac tttaagtggc 840

aagcactatt ctcgcagcca cgaagaatgg ctgaaaaaca tggataagaa tattgtggaa 900

tttaaggaaa ttatgcgtag tatcaccaaa accgaaaaag aagccattaa actgttaaac 960

ttctggcgta ttttctgcat gtgcggcgcc gagctgtttg gctataaaaa cggcgaggag 1020

tggatgttaa cccatctgct gtttaagaaa aaataa 1056

<210> 30

<211> 351

<212> PRT

<213> Unkown

<400> 30

Met Gln Leu Lys Glu Lys Glu Glu Leu Leu Arg Asn Met Glu Leu Gly

1 5 10 15

Leu Ile Pro Asp Gln Glu Ile Arg Gln Arg Ile Arg Ile Glu Leu Glu

20 25 30

Lys Arg Leu Gln Trp Gly Tyr Lys Glu Thr His Glu Glu Gln Leu Ser

35 40 45

Gln Leu Leu Asp Leu Val His Ser Leu Lys Gly Met Lys Met Ala Thr

50 55 60

Glu Met Glu Asn Leu Asp Leu Lys Leu Tyr Glu Ala Pro Met Glu Phe

65 70 75 80

Leu Lys Ile Gln His Gly Ser Asn Met Lys Gln Ser Ala Gly Tyr Tyr

85 90 95

Thr Asp Glu Ser Thr Thr Leu Asp Glu Ala Glu Ile Ala Met Leu Asp

100 105 110

Leu Tyr Met Glu Arg Ala Gln Ile Lys Asp Gly Gln Ser Val Leu Asp

115 120 125

Leu Gly Cys Gly Leu Gly Ala Val Ala Leu Phe Gly Ala Asn Lys Phe

130 135 140

Lys Lys Cys Gln Phe Thr Gly Val Thr Ser Ser Val Glu Gln Lys Asp

145 150 155 160

Tyr Ile Glu Gly Lys Cys Lys Glu Leu Lys Leu Thr Asn Val Lys Val

165 170 175

Leu Leu Ala Asp Ile Thr Thr Tyr Glu Thr Glu Glu Arg Phe Asp Arg

180 185 190

Ile Phe Ala Val Glu Leu Ile Glu His Met Lys Asn Tyr Gln Leu Leu

195 200 205

Leu Lys Lys Ile Ser Glu Trp Met Lys Asp Asp Gly Leu Leu Phe Val

210 215 220

Glu His Val Cys His Lys Thr Leu Ala Tyr His Tyr Glu Pro Val Asp

225 230 235 240

Ala Glu Asp Trp Tyr Thr Asn Tyr Ile Phe Pro Ala Gly Thr Leu Thr

245 250 255

Leu Ser Ser Ala Ser Met Leu Leu Tyr Phe Gln Asp Asp Val Ser Val

260 265 270

Val Asn Gln Trp Thr Leu Ser Gly Lys His Tyr Ser Arg Ser His Glu

275 280 285

Glu Trp Leu Lys Asn Met Asp Lys Asn Ile Val Glu Phe Lys Glu Ile

290 295 300

Met Arg Ser Ile Thr Lys Thr Glu Lys Glu Ala Ile Lys Leu Leu Asn

305 310 315 320

Phe Trp Arg Ile Phe Cys Met Cys Gly Ala Glu Leu Phe Gly Tyr Lys

325 330 335

Asn Gly Glu Glu Trp Met Leu Thr His Leu Leu Phe Lys Lys Lys

340 345 350

<210> 31

<211> 1563

<212> DNA

<213> Unkown

<400> 31

atgaaaccag aagatttccg cgccagtacc caacgtcctt tcaccgggga agagtatctg 60

aaaagcctgc aggatggtcg cgagatctat atctatggcg agcgagtgaa agacgtcacc 120

actcatccgg catttcgtaa tgcggcagcg tctgttgccc agctgtacga cgcactgcac 180

aaaccggaga tgcaggactc tctgtgttgg aacaccgaca ccggcagcgg cggctatacc 240

cataaattct tccgcgtggc gaaaagtgcc gacgacctgc gccagcaacg cgacgccatc 300

gctgagtggt cacgcctgag ctatggctgg atgggccgta ccccagacta caaagccgct 360

ttcggttgcg cactgggcgc gaatccgggc ttttacggtc agttcgagca gaacgcccgt 420

aactggtaca cccgtattca ggaaactggc ctctacttta accacgcgat tgttaaccca 480

ccgatcgatc gtcatttgcc gaccgataaa gtgaaagacg tttacatcaa gctggaaaaa 540

gagactgacg ccgggattat cgtcagcggt gcgaaagtgg ttgccaccaa ctcggcgctg 600

actcactaca acatgattgg cttcggctcg gcacaagtga tgggcgaaaa cccggacttc 660

gcactgatgt tcgttgcgcc aatggatgcc gatggcgtga aattaatctc ccgcgcctct 720

tatgagatgg tcgcgggtgc taccggctcg ccatacgact acccgctctc cagccgcttc 780

gatgagaacg atgcgattct ggtgatggat aacgtgctga ttccatggga aaacgtgctg 840

atctaccgcg attttgatcg ctgccgtcgc tggacgatgg aaggcggttt tgcccgtatg 900

tatccgctgc aagcctgtgt gcgcctggca gtgaaattag acttcattac ggcactgctg 960

aaaaaatcac tcgaatgtac cggcaccctg gagttccgtg gtgtgcaggc cgatctcggt 1020

gaagtggtag cgtggcgcaa caccttctgg gcattgagtg actcgatgtg ttcagaagca 1080

acgccgtggg tcaacggggc ttatttaccg gatcatgccg cactgcaaac ctatcgcgta 1140

ctggcaccaa tggcctacgc gaagatcaaa aacattatcg aacgcaacgt taccagtggc 1200

ctgatctatc tcccttccag tgcccgtgac ctgaataatc cgcagatcga ccagtatctg 1260

gcgaagtatg tgcgcggttc gaacggtatg gatcacgtcc agcgcatcaa gatcctcaaa 1320

ctgatgtggg atgctattgg cagcgaattt ggtggtcgtc acgaactgta tgaaatcaac 1380

tactccggta gccaggatga gattcgcctg cagtgtctgc gccaggcaca aaactccggc 1440

aatatggaca agatgatggc gatggttgat cgctgcctgt cggaatacga ccaggacggc 1500

tggactgtgc cgcacctgca caacaacgac gatatcaaca tgctggataa gctgctgaaa 1560

taa 1563

<210> 32

<211> 520

<212> PRT

<213> Unkown

<400> 32

Met Lys Pro Glu Asp Phe Arg Ala Ser Thr Gln Arg Pro Phe Thr Gly

1 5 10 15

Glu Glu Tyr Leu Lys Ser Leu Gln Asp Gly Arg Glu Ile Tyr Ile Tyr

20 25 30

Gly Glu Arg Val Lys Asp Val Thr Thr His Pro Ala Phe Arg Asn Ala

35 40 45

Ala Ala Ser Val Ala Gln Leu Tyr Asp Ala Leu His Lys Pro Glu Met

50 55 60

Gln Asp Ser Leu Cys Trp Asn Thr Asp Thr Gly Ser Gly Gly Tyr Thr

65 70 75 80

His Lys Phe Phe Arg Val Ala Lys Ser Ala Asp Asp Leu Arg Gln Gln

85 90 95

Arg Asp Ala Ile Ala Glu Trp Ser Arg Leu Ser Tyr Gly Trp Met Gly

100 105 110

Arg Thr Pro Asp Tyr Lys Ala Ala Phe Gly Cys Ala Leu Gly Ala Asn

115 120 125

Pro Gly Phe Tyr Gly Gln Phe Glu Gln Asn Ala Arg Asn Trp Tyr Thr

130 135 140

Arg Ile Gln Glu Thr Gly Leu Tyr Phe Asn His Ala Ile Val Asn Pro

145 150 155 160

Pro Ile Asp Arg His Leu Pro Thr Asp Lys Val Lys Asp Val Tyr Ile

165 170 175

Lys Leu Glu Lys Glu Thr Asp Ala Gly Ile Ile Val Ser Gly Ala Lys

180 185 190

Val Val Ala Thr Asn Ser Ala Leu Thr His Tyr Asn Met Ile Gly Phe

195 200 205

Gly Ser Ala Gln Val Met Gly Glu Asn Pro Asp Phe Ala Leu Met Phe

210 215 220

Val Ala Pro Met Asp Ala Asp Gly Val Lys Leu Ile Ser Arg Ala Ser

225 230 235 240

Tyr Glu Met Val Ala Gly Ala Thr Gly Ser Pro Tyr Asp Tyr Pro Leu

245 250 255

Ser Ser Arg Phe Asp Glu Asn Asp Ala Ile Leu Val Met Asp Asn Val

260 265 270

Leu Ile Pro Trp Glu Asn Val Leu Ile Tyr Arg Asp Phe Asp Arg Cys

275 280 285

Arg Arg Trp Thr Met Glu Gly Gly Phe Ala Arg Met Tyr Pro Leu Gln

290 295 300

Ala Cys Val Arg Leu Ala Val Lys Leu Asp Phe Ile Thr Ala Leu Leu

305 310 315 320

Lys Lys Ser Leu Glu Cys Thr Gly Thr Leu Glu Phe Arg Gly Val Gln

325 330 335

Ala Asp Leu Gly Glu Val Val Ala Trp Arg Asn Thr Phe Trp Ala Leu

340 345 350

Ser Asp Ser Met Cys Ser Glu Ala Thr Pro Trp Val Asn Gly Ala Tyr

355 360 365

Leu Pro Asp His Ala Ala Leu Gln Thr Tyr Arg Val Leu Ala Pro Met

370 375 380

Ala Tyr Ala Lys Ile Lys Asn Ile Ile Glu Arg Asn Val Thr Ser Gly

385 390 395 400

Leu Ile Tyr Leu Pro Ser Ser Ala Arg Asp Leu Asn Asn Pro Gln Ile

405 410 415

Asp Gln Tyr Leu Ala Lys Tyr Val Arg Gly Ser Asn Gly Met Asp His

420 425 430

Val Gln Arg Ile Lys Ile Leu Lys Leu Met Trp Asp Ala Ile Gly Ser

435 440 445

Glu Phe Gly Gly Arg His Glu Leu Tyr Glu Ile Asn Tyr Ser Gly Ser

450 455 460

Gln Asp Glu Ile Arg Leu Gln Cys Leu Arg Gln Ala Gln Asn Ser Gly

465 470 475 480

Asn Met Asp Lys Met Met Ala Met Val Asp Arg Cys Leu Ser Glu Tyr

485 490 495

Asp Gln Asp Gly Trp Thr Val Pro His Leu His Asn Asn Asp Asp Ile

500 505 510

Asn Met Leu Asp Lys Leu Leu Lys

515 520

<210> 33

<211> 513

<212> DNA

<213> Unkown

<400> 33

atgcaattag atgaacaacg cctgcgcttt cgtgacgcga tggccagcct gtcggcagcg 60

gtaaatatta tcaccaccga gggcgacgcc ggacaatgcg ggattacggc aacggccgtc 120

tgctcggtca cggatacacc accgtcgctg atggtgtgca ttaacgccaa cagtgcgatg 180

aacccggttt ttcagggcaa cggcaagttg tgcgtcaacg tcctcaacca tgagcaggaa 240

ctgatggcac gccacttcgc gggcatgaca ggcatggcga tggaagagcg ttttagcctc 300

tcatgctggc aaaaaggtcc gctggcgcag ccggtgctaa aaggttcgct ggccagtctt 360

gaaggtgaga tccgcgatgt gcaggcaatt ggcacacatc tggtgtatct ggtggagatt 420

aaaaacatca tcctcagtgc agaaggtcat ggacttatct actttaaacg ccgtttccat 480

ccggtgatgc tggaaatgga agctgcgatt taa 513

<210> 34

<211> 170

<212> PRT

<213> Unkown

<400> 34

Met Gln Leu Asp Glu Gln Arg Leu Arg Phe Arg Asp Ala Met Ala Ser

1 5 10 15

Leu Ser Ala Ala Val Asn Ile Ile Thr Thr Glu Gly Asp Ala Gly Gln

20 25 30

Cys Gly Ile Thr Ala Thr Ala Val Cys Ser Val Thr Asp Thr Pro Pro

35 40 45

Ser Leu Met Val Cys Ile Asn Ala Asn Ser Ala Met Asn Pro Val Phe

50 55 60

Gln Gly Asn Gly Lys Leu Cys Val Asn Val Leu Asn His Glu Gln Glu

65 70 75 80

Leu Met Ala Arg His Phe Ala Gly Met Thr Gly Met Ala Met Glu Glu

85 90 95

Arg Phe Ser Leu Ser Cys Trp Gln Lys Gly Pro Leu Ala Gln Pro Val

100 105 110

Leu Lys Gly Ser Leu Ala Ser Leu Glu Gly Glu Ile Arg Asp Val Gln

115 120 125

Ala Ile Gly Thr His Leu Val Tyr Leu Val Glu Ile Lys Asn Ile Ile

130 135 140

Leu Ser Ala Glu Gly His Gly Leu Ile Tyr Phe Lys Arg Arg Phe His

145 150 155 160

Pro Val Met Leu Glu Met Glu Ala Ala Ile

165 170

<210> 35

<211> 3525

<212> DNA

<213> Unkown

<400> 35

atgagtccga tcacacgcga ggagcgcctg gagcgtcgta ttcaagacct gtacgccaat 60

gatccgcaat ttgccgcagc caagccggca acagcaatta ccgccgcaat cgaacgtccg 120

ggcctgcctc tgcctcagat tattgaaacc gttatgacag gctatgccga ccgtcctgca 180

ctggcccaac gtagtgtgga atttgtgaca gatgcaggca ccggtcacac caccttacgc 240

ctgctgccgc acttcgagac catcagttac ggcgaactgt gggatcgcat tagtgccctg 300

gccgatgttt taagcaccga gcagaccgtg aaaccgggtg atcgcgtttg cctgctgggt 360

ttcaacagcg tggattatgc aacaattgac atgaccctgg cccgcctggg tgcagtggcc 420

gtgccgctgc aaaccagcgc agcaattaca cagctgcagc ctattgtggc cgaaacccag 480

ccgaccatga ttgcagccag cgttgacgcc ttagcagatg ccacagagtt agcactgagt 540

ggtcagaccg caacacgcgt gctggttttc gaccatcatc gccaggttga tgcacatcgc 600

gccgcagttg aaagtgcacg cgaacgcctg gccggcagtg cagtggttga gaccttagca 660

gaagccattg cacgtggtga cgtgccgcgt ggtgcaagtg caggtagtgc cccgggcaca 720

gacgttagtg atgacagtct ggccctgctg atttacacca gtggcagtac aggcgccccg 780

aagggtgcaa tgtatcctcg tcgcaatgtt gccacattct ggcgcaaacg cacctggttt 840

gaaggtggtt acgaaccgag tatcaccctg aacttcatgc cgatgagcca tgtgatgggt 900

cgccagatcc tgtacggcac cctgtgcaat ggcggcaccg cctacttcgt tgcaaagagc 960

gatctgagca cactgtttga agacctggca ttagtgcgcc cgaccgaatt aaccttcgtg 1020

ccgcgtgttt gggacatggt tttcgatgaa tttcagagcg aagttgaccg ccgcctggtt 1080

gatggtgcag atcgtgttgc cctggaggcc caggttaaag ccgagatccg taatgacgtg 1140

ttaggcggcc gctatacaag cgcactgacc ggcagtgcac cgattagcga tgagatgaag 1200

gcctgggttg aagagctgct ggacatgcac ctggtggaag gttacggcag tacagaagcc 1260

ggcatgatcc tgattgatgg cgcaattcgt cgccctgcag ttctggacta caaactggtg 1320

gatgtgccgg acctgggtta cttcttaacc gaccgccctc atccgcgcgg tgaactgctg 1380

gtgaagaccg acagcttatt ccctggttat taccagcgcg ccgaggttac cgcagacgtt 1440

tttgacgccg atggtttcta tcgcaccggc gacattatgg ccgaagtggg ccctgaacag 1500

ttcgtgtacc tggatcgtcg taataacgtt ctgaaattaa gtcagggcga gtttgttaca 1560

gtgagcaaac tggaggcagt gttcggtgac agcccgctgg tgcgtcagat ctatatctat 1620

ggcaacagtg cccgcgcata cctgctggca gtgatcgtgc ctacccaaga ggccctggat 1680

gccgttcctg ttgaggaatt aaaagcccgc ctgggcgata gcctgcagga agtggccaaa 1740

gcagccggtc tgcaaagtta cgaaatcccg cgcgacttca ttatcgaaac caccccgtgg 1800

acactggaga acggcctgct gacaggtatc cgtaaactgg cccgcccgca gctgaagaag 1860

cactatggcg agctgctgga gcagatctat acagacctgg cccacggcca agccgatgag 1920

ctgcgtagcc tgcgccagag tggtgccgat gcacctgtgc tggtgaccgt ttgtcgcgca 1980

gcagccgcct tactgggtgg tagcgccagc gatgtgcagc ctgacgccca cttcacagac 2040

ctgggcggtg atagcttaag tgccctgagt tttaccaacc tgctgcacga aatcttcgat 2100

atcgaggtgc ctgttggtgt gattgttagc ccggccaacg atttacaggc actggccgat 2160

tatgttgagg cagcacgtaa acctggcagc agtcgcccga cattcgccag cgttcatggt 2220

gccagcaatg gccaagtgac agaggtgcat gccggcgacc tgagtctgga taaatttatc 2280

gacgccgcca ccctggccga agcacctcgt ttaccggcag ccaataccca ggttcgcaca 2340

gtgctgctga ccggcgccac aggttttctg ggccgttatc tggcattaga gtggctggag 2400

cgtatggatc tggttgacgg caagctgatc tgcctggttc gcgcaaagag tgacacagaa 2460

gcccgtgcac gtttagacaa gacattcgac agcggcgatc cggaactgtt agcccactat 2520

cgcgcattag caggcgatca tctggaagtg ctggcaggtg ataagggcga agccgatctg 2580

ggtctggacc gccagacctg gcaacgctta gcagacacag tggacctgat cgttgatcct 2640

gcagcactgg tgaaccacgt gctgccgtat agtcagctgt ttggcccgaa tgccctgggt 2700

accgcagaac tgctgcgcct ggccctgacc agtaaaatta agccgtatag ttacacaagc 2760

accattggcg tggccgacca aattcctccg agcgcattta ccgaggatgc cgatatccgc 2820

gttattagtg ccacacgtgc agttgacgac agttacgcaa atggttacag caacagcaag 2880

tgggccggcg aggttctgct gcgcgaagca catgacctgt gcggcctgcc ggttgccgtg 2940

tttcgttgcg atatgatcct ggccgatacc acctgggcag gtcagttaaa cgtgccggat 3000

atgttcaccc gcatgatttt aagcttagcc gccaccggta tcgccccggg tagcttttat 3060

gagctggcag cagatggcgc ccgtcaacgc gcccactatg atggcctgcc ggtggagttt 3120

atcgcagagg ccattagcac actgggtgca cagagccagg acggcttcca tacctatcac 3180

gtgatgaatc cgtatgatga cggcatcggt ctggacgagt tcgttgattg gctgaacgaa 3240

agcggctgtc ctatccagcg cattgccgac tacggcgact ggctgcaacg tttcgagacc 3300

gcactgcgtg ccctgcctga tcgtcagcgc cacagtagtc tgttaccttt actgcacaac 3360

taccgtcagc ctgaacgtcc ggttcgcggt agcattgcac cgaccgaccg ttttcgcgca 3420

gccgtgcaag aggcgaagat tggcccggat aaagatattc cgcacgtggg cgccccgatc 3480

atcgtgaaat atgtgagtga cctgcgcctg ttaggcctgc tgtaa 3525

<210> 36

<211> 1174

<212> PRT

<213> Unkown

<400> 36

Met Ser Pro Ile Thr Arg Glu Glu Arg Leu Glu Arg Arg Ile Gln Asp

1 5 10 15

Leu Tyr Ala Asn Asp Pro Gln Phe Ala Ala Ala Lys Pro Ala Thr Ala

20 25 30

Ile Thr Ala Ala Ile Glu Arg Pro Gly Leu Pro Leu Pro Gln Ile Ile

35 40 45

Glu Thr Val Met Thr Gly Tyr Ala Asp Arg Pro Ala Leu Ala Gln Arg

50 55 60

Ser Val Glu Phe Val Thr Asp Ala Gly Thr Gly His Thr Thr Leu Arg

65 70 75 80

Leu Leu Pro His Phe Glu Thr Ile Ser Tyr Gly Glu Leu Trp Asp Arg

85 90 95

Ile Ser Ala Leu Ala Asp Val Leu Ser Thr Glu Gln Thr Val Lys Pro

100 105 110

Gly Asp Arg Val Cys Leu Leu Gly Phe Asn Ser Val Asp Tyr Ala Thr

115 120 125

Ile Asp Met Thr Leu Ala Arg Leu Gly Ala Val Ala Val Pro Leu Gln

130 135 140

Thr Ser Ala Ala Ile Thr Gln Leu Gln Pro Ile Val Ala Glu Thr Gln

145 150 155 160

Pro Thr Met Ile Ala Ala Ser Val Asp Ala Leu Ala Asp Ala Thr Glu

165 170 175

Leu Ala Leu Ser Gly Gln Thr Ala Thr Arg Val Leu Val Phe Asp His

180 185 190

His Arg Gln Val Asp Ala His Arg Ala Ala Val Glu Ser Ala Arg Glu

195 200 205

Arg Leu Ala Gly Ser Ala Val Val Glu Thr Leu Ala Glu Ala Ile Ala

210 215 220

Arg Gly Asp Val Pro Arg Gly Ala Ser Ala Gly Ser Ala Pro Gly Thr

225 230 235 240

Asp Val Ser Asp Asp Ser Leu Ala Leu Leu Ile Tyr Thr Ser Gly Ser

245 250 255

Thr Gly Ala Pro Lys Gly Ala Met Tyr Pro Arg Arg Asn Val Ala Thr

260 265 270

Phe Trp Arg Lys Arg Thr Trp Phe Glu Gly Gly Tyr Glu Pro Ser Ile

275 280 285

Thr Leu Asn Phe Met Pro Met Ser His Val Met Gly Arg Gln Ile Leu

290 295 300

Tyr Gly Thr Leu Cys Asn Gly Gly Thr Ala Tyr Phe Val Ala Lys Ser

305 310 315 320

Asp Leu Ser Thr Leu Phe Glu Asp Leu Ala Leu Val Arg Pro Thr Glu

325 330 335

Leu Thr Phe Val Pro Arg Val Trp Asp Met Val Phe Asp Glu Phe Gln

340 345 350

Ser Glu Val Asp Arg Arg Leu Val Asp Gly Ala Asp Arg Val Ala Leu

355 360 365

Glu Ala Gln Val Lys Ala Glu Ile Arg Asn Asp Val Leu Gly Gly Arg

370 375 380

Tyr Thr Ser Ala Leu Thr Gly Ser Ala Pro Ile Ser Asp Glu Met Lys

385 390 395 400

Ala Trp Val Glu Glu Leu Leu Asp Met His Leu Val Glu Gly Tyr Gly

405 410 415

Ser Thr Glu Ala Gly Met Ile Leu Ile Asp Gly Ala Ile Arg Arg Pro

420 425 430

Ala Val Leu Asp Tyr Lys Leu Val Asp Val Pro Asp Leu Gly Tyr Phe

435 440 445

Leu Thr Asp Arg Pro His Pro Arg Gly Glu Leu Leu Val Lys Thr Asp

450 455 460

Ser Leu Phe Pro Gly Tyr Tyr Gln Arg Ala Glu Val Thr Ala Asp Val

465 470 475 480

Phe Asp Ala Asp Gly Phe Tyr Arg Thr Gly Asp Ile Met Ala Glu Val

485 490 495

Gly Pro Glu Gln Phe Val Tyr Leu Asp Arg Arg Asn Asn Val Leu Lys

500 505 510

Leu Ser Gln Gly Glu Phe Val Thr Val Ser Lys Leu Glu Ala Val Phe

515 520 525

Gly Asp Ser Pro Leu Val Arg Gln Ile Tyr Ile Tyr Gly Asn Ser Ala

530 535 540

Arg Ala Tyr Leu Leu Ala Val Ile Val Pro Thr Gln Glu Ala Leu Asp

545 550 555 560

Ala Val Pro Val Glu Glu Leu Lys Ala Arg Leu Gly Asp Ser Leu Gln

565 570 575

Glu Val Ala Lys Ala Ala Gly Leu Gln Ser Tyr Glu Ile Pro Arg Asp

580 585 590

Phe Ile Ile Glu Thr Thr Pro Trp Thr Leu Glu Asn Gly Leu Leu Thr

595 600 605

Gly Ile Arg Lys Leu Ala Arg Pro Gln Leu Lys Lys His Tyr Gly Glu

610 615 620

Leu Leu Glu Gln Ile Tyr Thr Asp Leu Ala His Gly Gln Ala Asp Glu

625 630 635 640

Leu Arg Ser Leu Arg Gln Ser Gly Ala Asp Ala Pro Val Leu Val Thr

645 650 655

Val Cys Arg Ala Ala Ala Ala Leu Leu Gly Gly Ser Ala Ser Asp Val

660 665 670

Gln Pro Asp Ala His Phe Thr Asp Leu Gly Gly Asp Ser Leu Ser Ala

675 680 685

Leu Ser Phe Thr Asn Leu Leu His Glu Ile Phe Asp Ile Glu Val Pro

690 695 700

Val Gly Val Ile Val Ser Pro Ala Asn Asp Leu Gln Ala Leu Ala Asp

705 710 715 720

Tyr Val Glu Ala Ala Arg Lys Pro Gly Ser Ser Arg Pro Thr Phe Ala

725 730 735

Ser Val His Gly Ala Ser Asn Gly Gln Val Thr Glu Val His Ala Gly

740 745 750

Asp Leu Ser Leu Asp Lys Phe Ile Asp Ala Ala Thr Leu Ala Glu Ala

755 760 765

Pro Arg Leu Pro Ala Ala Asn Thr Gln Val Arg Thr Val Leu Leu Thr

770 775 780

Gly Ala Thr Gly Phe Leu Gly Arg Tyr Leu Ala Leu Glu Trp Leu Glu

785 790 795 800

Arg Met Asp Leu Val Asp Gly Lys Leu Ile Cys Leu Val Arg Ala Lys

805 810 815

Ser Asp Thr Glu Ala Arg Ala Arg Leu Asp Lys Thr Phe Asp Ser Gly

820 825 830

Asp Pro Glu Leu Leu Ala His Tyr Arg Ala Leu Ala Gly Asp His Leu

835 840 845

Glu Val Leu Ala Gly Asp Lys Gly Glu Ala Asp Leu Gly Leu Asp Arg

850 855 860

Gln Thr Trp Gln Arg Leu Ala Asp Thr Val Asp Leu Ile Val Asp Pro

865 870 875 880

Ala Ala Leu Val Asn His Val Leu Pro Tyr Ser Gln Leu Phe Gly Pro

885 890 895

Asn Ala Leu Gly Thr Ala Glu Leu Leu Arg Leu Ala Leu Thr Ser Lys

900 905 910

Ile Lys Pro Tyr Ser Tyr Thr Ser Thr Ile Gly Val Ala Asp Gln Ile

915 920 925

Pro Pro Ser Ala Phe Thr Glu Asp Ala Asp Ile Arg Val Ile Ser Ala

930 935 940

Thr Arg Ala Val Asp Asp Ser Tyr Ala Asn Gly Tyr Ser Asn Ser Lys

945 950 955 960

Trp Ala Gly Glu Val Leu Leu Arg Glu Ala His Asp Leu Cys Gly Leu

965 970 975

Pro Val Ala Val Phe Arg Cys Asp Met Ile Leu Ala Asp Thr Thr Trp

980 985 990

Ala Gly Gln Leu Asn Val Pro Asp Met Phe Thr Arg Met Ile Leu Ser

995 1000 1005

Leu Ala Ala Thr Gly Ile Ala Pro Gly Ser Phe Tyr Glu Leu Ala Ala

1010 1015 1020

Asp Gly Ala Arg Gln Arg Ala His Tyr Asp Gly Leu Pro Val Glu Phe

1025 1030 1035 1040

Ile Ala Glu Ala Ile Ser Thr Leu Gly Ala Gln Ser Gln Asp Gly Phe

1045 1050 1055

His Thr Tyr His Val Met Asn Pro Tyr Asp Asp Gly Ile Gly Leu Asp

1060 1065 1070

Glu Phe Val Asp Trp Leu Asn Glu Ser Gly Cys Pro Ile Gln Arg Ile

1075 1080 1085

Ala Asp Tyr Gly Asp Trp Leu Gln Arg Phe Glu Thr Ala Leu Arg Ala

1090 1095 1100

Leu Pro Asp Arg Gln Arg His Ser Ser Leu Leu Pro Leu Leu His Asn

1105 1110 1115 1120

Tyr Arg Gln Pro Glu Arg Pro Val Arg Gly Ser Ile Ala Pro Thr Asp

1125 1130 1135

Arg Phe Arg Ala Ala Val Gln Glu Ala Lys Ile Gly Pro Asp Lys Asp

1140 1145 1150

Ile Pro His Val Gly Ala Pro Ile Ile Val Lys Tyr Val Ser Asp Leu

1155 1160 1165

Arg Leu Leu Gly Leu Leu

1170

<210> 37

<211> 675

<212> DNA

<213> Unkown

<400> 37

atgaagattt acggaattta tatggaccgc ccgctttcac aggaagaaaa tgaacggttc 60

atgactttca tatcacctga aaaacgggag aaatgccgga gattttatca taaagaagat 120

gctcaccgca ccctgctggg agatgtgctc gttcgctcag tcataagcag gcagtatcag 180

ttggacaaat ccgatatccg ctttagcacg caggaatacg ggaagccgtg catccctgat 240

cttcccgacg ctcatttcaa catttctcac tccggccgct gggtcattgg tgcgtttgat 300

tcacagccga tcggcataga tatcgaaaaa acgaaaccga tcagccttga gatcgccaag 360

cgcttctttt caaaaacaga gtacagcgac cttttagcaa aagacaagga cgagcagaca 420

gactattttt atcatctatg gtcaatgaaa gaaagcttta tcaaacagga aggcaaaggc 480

ttatcgcttc cgcttgattc cttttcagtg cgcctgcatc aggacggaca agtatccatt 540

gagcttccgg acagccattc cccatgctat atcaaaacgt atgaggtcga tcccggctac 600

aaaatggctg tatgcgccgc acaccctgat ttccccgagg atatcacaat ggtctcgtac 660

gaagagcttt tataa 675

<210> 38

<211> 224

<212> PRT

<213> Unkown

<400> 38

Met Lys Ile Tyr Gly Ile Tyr Met Asp Arg Pro Leu Ser Gln Glu Glu

1 5 10 15

Asn Glu Arg Phe Met Thr Phe Ile Ser Pro Glu Lys Arg Glu Lys Cys

20 25 30

Arg Arg Phe Tyr His Lys Glu Asp Ala His Arg Thr Leu Leu Gly Asp

35 40 45

Val Leu Val Arg Ser Val Ile Ser Arg Gln Tyr Gln Leu Asp Lys Ser

50 55 60

Asp Ile Arg Phe Ser Thr Gln Glu Tyr Gly Lys Pro Cys Ile Pro Asp

65 70 75 80

Leu Pro Asp Ala His Phe Asn Ile Ser His Ser Gly Arg Trp Val Ile

85 90 95

Gly Ala Phe Asp Ser Gln Pro Ile Gly Ile Asp Ile Glu Lys Thr Lys

100 105 110

Pro Ile Ser Leu Glu Ile Ala Lys Arg Phe Phe Ser Lys Thr Glu Tyr

115 120 125

Ser Asp Leu Leu Ala Lys Asp Lys Asp Glu Gln Thr Asp Tyr Phe Tyr

130 135 140

His Leu Trp Ser Met Lys Glu Ser Phe Ile Lys Gln Glu Gly Lys Gly

145 150 155 160

Leu Ser Leu Pro Leu Asp Ser Phe Ser Val Arg Leu His Gln Asp Gly

165 170 175

Gln Val Ser Ile Glu Leu Pro Asp Ser His Ser Pro Cys Tyr Ile Lys

180 185 190

Thr Tyr Glu Val Asp Pro Gly Tyr Lys Met Ala Val Cys Ala Ala His

195 200 205

Pro Asp Phe Pro Glu Asp Ile Thr Met Val Ser Tyr Glu Glu Leu Leu

210 215 220

<210> 39

<211> 1488

<212> DNA

<213> Unkown

<400> 39

atgaattttc atcatctggc ttactggcag gataaagcgt taagtctcgc cattgaaaac 60

cgcttattta ttaacggtga atatactgct gcggcggaaa atgaaacctt tgaaaccgtt 120

gatccggtca cccaggcacc gctggcgaaa attgcccgcg gcaagagcgt cgatatcgac 180

cgtgcgatga gcgcagcacg cggcgtattt gaacgcggcg actggtcact ctcttctccg 240

gctaaacgta aagcggtact gaataaactc gccgatttaa tggaagccca cgccgaagag 300

ctggcactgc tggaaactct cgacaccggc aaaccgattc gtcacagtct gcgtgatgat 360

attcccggcg cggcgcgcgc cattcgctgg tacgccgaag cgatcgacaa agtgtatggc 420

gaagtggcga ccaccagtag ccatgagctg gcgatgatcg tgcgtgaacc ggtcggcgtg 480

attgccgcca tcgtgccgtg gaacttcccg ctgttgctga cttgctggaa actcggcccg 540

gcgctggcgg cgggaaacag cgtgattcta aaaccgtctg aaaaatcacc gctcagtgcg 600

attcgtctcg cggggctggc gaaagaagca ggcttgccgg atggtgtgtt gaacgtggtg 660

acgggttttg gtcatgaagc cgggcaggcg ctgtcgcgtc ataacgatat cgacgccatt 720

gcctttaccg gttcaacccg taccgggaaa cagctgctga aagatgcggg cgacagcaac 780

atgaaacgcg tctggctgga agcgggcggc aaaagcgcca acatcgtttt cgctgactgc 840

ccggatttgc aacaggcggc aagcgccacc gcagcaggca ttttctacaa ccagggacag 900

gtgtgcatcg ccggaacgcg cctgttgctg gaagagagca tcgccgatga attcttagcc 960

ctgttaaaac agcaggcgca aaactggcag ccgggccatc cacttgatcc cgcaaccacc 1020

atgggcacct taatcgactg cgcccacgcc gactcggtcc atagctttat tcgggaaggc 1080

gaaagcaaag ggcaactgtt gttggatggc cgtaacgccg ggctggctgc cgccatcggc 1140

ccgaccatct ttgtggatgt ggacccgaat gcgtccttaa gtcgcgaaga gattttcggt 1200

ccggtgctgg tggtcacgcg tttcacatca gaagaacagg cgctacagct tgccaacgac 1260

agccagtacg gccttggcgc ggcggtatgg acgcgcgacc tctcccgcgc gcaccgcatg 1320

agccgacgcc tgaaagccgg ttccgtcttc gtcaataact acaacgacgg cgatatgacc 1380

gtgccgtttg gcggctataa gcagagcggc aacggtcgcg acaaatccct gcatgccctt 1440

gaaaaattca ctgaactgaa aaccatctgg ataagcctgg aggcctga 1488

<210> 40

<211> 495

<212> PRT

<213> Unkown

<400> 40

Met Asn Phe His His Leu Ala Tyr Trp Gln Asp Lys Ala Leu Ser Leu

1 5 10 15

Ala Ile Glu Asn Arg Leu Phe Ile Asn Gly Glu Tyr Thr Ala Ala Ala

20 25 30

Glu Asn Glu Thr Phe Glu Thr Val Asp Pro Val Thr Gln Ala Pro Leu

35 40 45

Ala Lys Ile Ala Arg Gly Lys Ser Val Asp Ile Asp Arg Ala Met Ser

50 55 60

Ala Ala Arg Gly Val Phe Glu Arg Gly Asp Trp Ser Leu Ser Ser Pro

65 70 75 80

Ala Lys Arg Lys Ala Val Leu Asn Lys Leu Ala Asp Leu Met Glu Ala

85 90 95

His Ala Glu Glu Leu Ala Leu Leu Glu Thr Leu Asp Thr Gly Lys Pro

100 105 110

Ile Arg His Ser Leu Arg Asp Asp Ile Pro Gly Ala Ala Arg Ala Ile

115 120 125

Arg Trp Tyr Ala Glu Ala Ile Asp Lys Val Tyr Gly Glu Val Ala Thr

130 135 140

Thr Ser Ser His Glu Leu Ala Met Ile Val Arg Glu Pro Val Gly Val

145 150 155 160

Ile Ala Ala Ile Val Pro Trp Asn Phe Pro Leu Leu Leu Thr Cys Trp

165 170 175

Lys Leu Gly Pro Ala Leu Ala Ala Gly Asn Ser Val Ile Leu Lys Pro

180 185 190

Ser Glu Lys Ser Pro Leu Ser Ala Ile Arg Leu Ala Gly Leu Ala Lys

195 200 205

Glu Ala Gly Leu Pro Asp Gly Val Leu Asn Val Val Thr Gly Phe Gly

210 215 220

His Glu Ala Gly Gln Ala Leu Ser Arg His Asn Asp Ile Asp Ala Ile

225 230 235 240

Ala Phe Thr Gly Ser Thr Arg Thr Gly Lys Gln Leu Leu Lys Asp Ala

245 250 255

Gly Asp Ser Asn Met Lys Arg Val Trp Leu Glu Ala Gly Gly Lys Ser

260 265 270

Ala Asn Ile Val Phe Ala Asp Cys Pro Asp Leu Gln Gln Ala Ala Ser

275 280 285

Ala Thr Ala Ala Gly Ile Phe Tyr Asn Gln Gly Gln Val Cys Ile Ala

290 295 300

Gly Thr Arg Leu Leu Leu Glu Glu Ser Ile Ala Asp Glu Phe Leu Ala

305 310 315 320

Leu Leu Lys Gln Gln Ala Gln Asn Trp Gln Pro Gly His Pro Leu Asp

325 330 335

Pro Ala Thr Thr Met Gly Thr Leu Ile Asp Cys Ala His Ala Asp Ser

340 345 350

Val His Ser Phe Ile Arg Glu Gly Glu Ser Lys Gly Gln Leu Leu Leu

355 360 365

Asp Gly Arg Asn Ala Gly Leu Ala Ala Ala Ile Gly Pro Thr Ile Phe

370 375 380

Val Asp Val Asp Pro Asn Ala Ser Leu Ser Arg Glu Glu Ile Phe Gly

385 390 395 400

Pro Val Leu Val Val Thr Arg Phe Thr Ser Glu Glu Gln Ala Leu Gln

405 410 415

Leu Ala Asn Asp Ser Gln Tyr Gly Leu Gly Ala Ala Val Trp Thr Arg

420 425 430

Asp Leu Ser Arg Ala His Arg Met Ser Arg Arg Leu Lys Ala Gly Ser

435 440 445

Val Phe Val Asn Asn Tyr Asn Asp Gly Asp Met Thr Val Pro Phe Gly

450 455 460

Gly Tyr Lys Gln Ser Gly Asn Gly Arg Asp Lys Ser Leu His Ala Leu

465 470 475 480

Glu Lys Phe Thr Glu Leu Lys Thr Ile Trp Ile Ser Leu Glu Ala

485 490 495

<210> 41

<211> 60

<212> DNA

<213> Unkown

<400> 41

atggctatcc ctgcatttgg tttaggtact ttccgtctga gtgtaggctg gagctgcttc 60

<210> 42

<211> 62

<212> DNA

<213> Unkown

<400> 42

ttaatcccat tcaggagcca gaccttccgg gctaaccagg cggcatatga atatcctcct 60

ta 62

<210> 43

<211> 62

<212> DNA

<213> Unkown

<400> 43

atgcaacaaa aaatgattca atttagtggc gatgtctcac tggtgtaggc tggagctgct 60

tc 62

<210> 44

<211> 62

<212> DNA

<213> Unkown

<400> 44

tcacaccata tccagcgcag tttttccttt tggtgccgga tatcatatga atatcctcct 60

ta 62

<210> 45

<211> 64

<212> DNA

<213> Unkown

<400> 45

atgctacaaa attgcgcaca atcaaattgc cgcattattc ctaagtgtag gctggagctg 60

cttc 64

<210> 46

<211> 61

<212> DNA

<213> Unkown

<400> 46

ttaattcccc tgcatcgccc gcattcttgc cgcatcttcc cccatatgaa tatcctcctt 60

a 61

<210> 47

<211> 61

<212> DNA

<213> Unkown

<400> 47

atgaacaact ttaatctgca caccccaacc cgcattctgt tgtgtaggct ggagctgctt 60

c 61

<210> 48

<211> 60

<212> DNA

<213> Unkown

<400> 48

ttagcgggcg gcttcgtata tacggcggct gacatccaac gcatatgaat atcctcctta 60

<210> 49

<211> 62

<212> DNA

<213> Unkown

<400> 49

atggctaatc caaccgttat taagctacag gatggcaatg tcgtgtaggc tggagctgct 60

tc 62

<210> 50

<211> 60

<212> DNA

<213> Unkown

<400> 50

ttagccgccg aactggtcag gatcgggacc gagacgcttg ccatatgaat atcctcctta 60

<210> 51

<211> 61

<212> DNA

<213> Unkown

<400> 51

atgaagatca aagctgttgg tgcatattcc gctaaacaac cgtgtaggct ggagctgctt 60

c 61

<210> 52

<211> 60

<212> DNA

<213> Unkown

<400> 52

tcagtctgtt agtgtgcgat tatcgataac aaaacgatat tcatatgaat atcctcctta 60

<210> 53

<211> 62

<212> DNA

<213> Unkown

<400> 53

atgtcgatga taaaaagcta tgccgcaaaa gaagcgggcg gcgtgtaggc tggagctgct 60

tc 62

<210> 54

<211> 63

<212> DNA

<213> Unkown

<400> 54

tcaaaaatcg gctttcaaca ccacgcggta acgcgcctta ccgtcatatg aatatcctcc 60

tta 63

<210> 55

<211> 49

<212> DNA

<213> Unkown

<400> 55

gtatctagaa agaggagata taatgaatta tcagaacgac gatttacgc 49

<210> 56

<211> 35

<212> DNA

<213> Unkown

<400> 56

tatggatcca ctagtttacc cgcgacgcgc tttta 35

<210> 57

<211> 44

<212> DNA

<213> Unkown

<400> 57

gtatctagaa agaggagata taatggttgc tgaattgacc gcat 44

<210> 58

<211> 38

<212> DNA

<213> Unkown

<400> 58

atatgtcgac actagtttac tggcgattgt cattcgcc 38

<210> 59

<211> 53

<212> DNA

<213> Unkown

<400> 59

atctctagat ttaagaagga gatataatgg cacctgttac aattgaaaag ttc 53

<210> 60

<211> 44

<212> DNA

<213> Unkown

<400> 60

tctggatccg ctagcctatt ttttatttct tttaagtgcc gctg 44

<210> 61

<211> 46

<212> DNA

<213> Unkown

<400> 61

tagtctagat ttaagaagga gatataatga cccccgaaca gttccg 46

<210> 62

<211> 38

<212> DNA

<213> Unkown

<400> 62

acaggatcca ctagtttagc tgccgccttt aatcacgt 38

<210> 63

<211> 54

<212> DNA

<213> Unkown

<400> 63

tactctagat ttaagaagga gatataatgc gtaaagtgat caaatatgat atgg 54

<210> 64

<211> 38

<212> DNA

<213> Unkown

<400> 64

tcaggatcca ctagtttagc tgcccagtaa acgaggaa 38

<210> 65

<211> 51

<212> DNA

<213> Unkown

<400> 65

ttgtctagat ttaagaagga gatataatgg agaccgttag caaaattgac c 51

<210> 66

<211> 42

<212> DNA

<213> Unkown

<400> 66

gcaggatcca ctagtttagt acggatatgc ttcaatcacg ct 42

<210> 67

<211> 47

<212> DNA

<213> Unkown

<400> 67

tactctagat ttaagaagga gatataatgg ccttccatgg caaggac 47

<210> 68

<211> 41

<212> DNA

<213> Unkown

<400> 68

tcaggatcca ctagtttaag gaaacacctc aatcacgctc t 41

<210> 69

<211> 53

<212> DNA

<213> Unkown

<400> 69

atctctagat ttaagaagga gatataatgc agttaaaaga aaaagaggag ctg 53

<210> 70

<211> 46

<212> DNA

<213> Unkown

<400> 70

agaggatcca ctagtttatt ttttcttaaa cagcagatgg gttaac 46

<210> 71

<211> 48

<212> DNA

<213> Unkown

<400> 71

tagtctagat ttaagaagga gatataatga aaccagaaga tttccgcg 48

<210> 72

<211> 33

<212> DNA

<213> Unkown

<400> 72

aacgctcgag ttaaatcgca gcttccattt cca 33

<210> 73

<211> 47

<212> DNA

<213> Unkown

<400> 73

atctctagat ttaagaagga gatataatga gtccgatcac acgcgag 47

<210> 74

<211> 36

<212> DNA

<213> Unkown

<400> 74

agaggatcca ctagtttaca gcaggcctaa caggcg 36

<210> 75

<211> 55

<212> DNA

<213> Unkown

<400> 75

gtctctagat ttaagaagga gatataatga agatttacgg aatttatatg gaccg 55

<210> 76

<211> 45

<212> DNA

<213> Unkown

<400> 76

tgaggatcca ctagtttata aaagctcttc gtacgagacc attgt 45

<210> 77

<211> 52

<212> DNA

<213> Unkown

<400> 77

atctctagat ttaagaagga gatataatga attttcatca tctggcttac tg 52

<210> 78

<211> 34

<212> DNA

<213> Unkown

<400> 78

tcaggatccg ctagctcagg cctccaggct atcc 34

Claims (5)

1.一种微生物，其特征在于所述微生物不含有或者敲除dkgB、yeaE、yqhC、yqhD、dkgA、yahK和yjgB基因，高表达或者导入aroG^fbr、tyrA^fb、KDC、HpaB、HpaC、DODC、CAR、sfp、aldH、CNS、4OMT、6OMT和CNMT基因；所述微生物为细菌、真菌、放线菌、螺旋体、支原体、衣原体或立克次体。

2.根据权利要求1所述的微生物，其特征在于：

所述dkgB基因具有SEQ ID NO：1所示的核苷酸序列或者所述dkgB基因编码SEQ ID NO：2所示的氨基酸序列；

所述yeaE基因具有SEQ ID NO：3所示的核苷酸序列或者所述yeaE基因编码SEQ ID NO：4所示的氨基酸序列；

所述yqhC基因具有SEQ ID NO：5所示的核苷酸序列或者所述yqhC基因编码SEQ ID NO：6所示的氨基酸序列；

所述yqhD基因具有SEQ ID NO：7所示的核苷酸序列或者所述yqhD基因编码SEQ ID NO：8所示的氨基酸序列；

所述dkgA基因具有SEQ ID NO：9所示的核苷酸序列或者所述dkgA基因编码SEQ ID NO：10所示的氨基酸序列；

所述yahK基因具有SEQ ID NO：11所示的核苷酸序列或者所述yahK基因编码SEQ IDNO：12所示的氨基酸序列；

所述yjgB基因具有SEQ ID NO：13所示的核苷酸序列或者所述yjgB基因编码SEQ IDNO：14所示的氨基酸序列；

所述aroG^fbr基因具有SEQ ID NO：15所示的核苷酸序列或者所述aroG^fbr基因编码SEQID NO：16所示的氨基酸序列；

所述tyrA^fb基因具有SEQ ID NO：17所示的核苷酸序列或者所述tyrA^fb基因编码SEQ IDNO：18所示的氨基酸序列；

所述KDC基因具有SEQ ID NO：19所示的核苷酸序列或者所述KDC基因编码SEQ ID NO：20所示的氨基酸序列；

所述DODC基因具有SEQ ID NO：21所示的核苷酸序列或者所述DODC基因编码SEQ IDNO：22所示的氨基酸序列；

所述CNS基因具有SEQ ID NO：23所示的核苷酸序列或者所述CNS基因编码SEQ ID NO：24所示的氨基酸序列；

所述6OMT基因具有SEQ ID NO：25所示的核苷酸序列或者所述6OMT基因编码SEQ IDNO：26所示的氨基酸序列；

所述4OMT基因具有SEQ ID NO：27所示的核苷酸序列或者所述4OMT基因编码SEQ IDNO：28所示的氨基酸序列；

所述CNMT基因具有SEQ ID NO：29所示的核苷酸序列或者所述NMCH基因编码SEQ IDNO：30所示的氨基酸序列；

所述HpaB基因具有SEQ ID NO：31所示的核苷酸序列或者所述HpaB基因编码SEQ IDNO：32所示的氨基酸序列；

所述HpaC基因具有SEQ ID NO：33所示的核苷酸序列或者所述HpaC基因编码SEQ IDNO：34所示的氨基酸序列；

所述CAR基因具有SEQ ID NO：35所示的核苷酸序列或者所述CAR基因编码SEQ ID NO：36所示的氨基酸序列；

所述sfp基因具有SEQ ID NO：37所示的核苷酸序列或者所述sfp基因编码SEQ ID NO：38所示的氨基酸序列；

所述aldH基因具有SEQ ID NO：39所示的核苷酸序列或者所述aldH基因编码SEQ IDNO：40所示的氨基酸序列。

3.根据权利要求1所述的微生物，其特征在于所述微生物为酵母或大肠杆菌。

4.权利要求1～3任意一项所述的微生物在生物合成牛心果碱中的应用。

5.一种微生物发酵葡萄糖合成牛心果碱的方法，其特征在于使用权利要求1～3中任意一项所述的微生物。

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