CN111217931A - Extraction process of angelica sinensis polysaccharide - Google Patents

Abstract

The invention discloses an extraction process of angelica polysaccharide, which comprises the following steps: washing radix Angelicae sinensis powder with pure water, draining, and extracting polysaccharide by orthogonal test design; extracting by taking the extraction temperature, the extraction time, the extraction times, the material-water ratio and the ultrasonic power as investigation factors; centrifuging after extraction is finished, removing filter residues, concentrating the obtained filtrate on a rotary evaporator under reduced pressure, and removing protein by a savage method; adding ethanol to make ethanol content reach 80%, standing at low temperature overnight, centrifuging, washing the residue with the solution for several times, drying to obtain gray powder extract, and determining polysaccharide content. The angelica polysaccharide has multiple functions of enhancing the activity of an immune system, resisting oxidation and the like. In recent years, the use value of plant polysaccharide is attracting more and more attention, and the plant polysaccharide is a good anti-cancer and antihypertensive drug.

Description

Extraction process of angelica sinensis polysaccharide

Technical Field

The invention relates to the technical field of biological extraction, in particular to an extraction process of angelica sinensis polysaccharide.

Background

Angelica sinensis (with the academic name of Angelica sinensis) is also called Angelica sinensis, Qibang, Xiangelicae sinensis, Minangelicae sinensis, Angelica sinensis, Yunqui, Angelica sinensis David, and Aralia cordata, and is herbaceous for many years and 0.4-1 m higher. The flowering period is 6-7 months, and the fruit period is 7-9 months.

European angelica was introduced from Europe in 1957 in China. The major production of southeast part of Gansu province has high yield and good quality in Min county, and is cultivated in Yunnan province, Sichuan province, Shanxi province, Hubei province and the like. Some provinces in China also introduce and cultivate.

The root of it can be used as a medicine, and is one of the most commonly used Chinese herbs. Has effects in nourishing blood, regulating menstruation, relieving pain, moistening dryness, smoothing intestine, resisting cancer, resisting aging, and enhancing immunity.

In recent years, the use value of plant polysaccharide is attracting more and more attention, the research on the comprehensive utilization of angelica polysaccharide is also small, the extraction of angelica flavone and polysaccharide and the comprehensive development and utilization of products are in the initial stage, and the development prospect is good.

Disclosure of Invention

Based on the above purposes, the invention provides an extraction process of angelica sinensis polysaccharide

The invention relates to an extraction process of angelica polysaccharide, and the preparation method comprises the following steps:

a. washing radix Angelicae sinensis powder with pure water, draining, and extracting polysaccharide by orthogonal test design;

b. extracting at 60 deg.C for 75min for 2 times at a material-water ratio of 1:35g/ml and ultrasonic power of 200W;

c. centrifuging after extraction is finished, removing filter residues, concentrating the obtained filtrate on a rotary evaporator under reduced pressure, and removing protein by a savage method;

d. adding 95% ethanol to make ethanol content reach 80%, standing at low temperature overnight, centrifuging, washing the residue with diethyl ether and acetone solution for 6 times, drying to obtain gray powder extract, and determining polysaccharide content.

The invention adopts a process method to extract angelica polysaccharide, which has the functions of enhancing the activity of an immune system, resisting oxidation and the like. In recent years, the use value of plant polysaccharide is attracting more and more attention, and the plant polysaccharide is a good medicine for resisting cancer and enhancing immunity.

Detailed Description

The present invention will be further described with reference to the following examples.

Example 1

1. Washing radix Angelicae sinensis powder with pure water, draining, and extracting polysaccharide by orthogonal test design;

2. extracting at 60 deg.C for 75min for 2 times at a material-water ratio of 1:35g/ml and ultrasonic power of 200W;

3. centrifuging after extraction is finished, removing filter residues, concentrating the obtained filtrate on a rotary evaporator under reduced pressure, and removing protein by a savage method;

4. adding 95% ethanol to make ethanol content reach 80%, standing at low temperature overnight, centrifuging, washing the residue with diethyl ether and acetone solution for 6 times, drying to obtain gray powder extract, and determining polysaccharide content.

Example 2

1. Washing radix Angelicae sinensis powder with pure water, draining, and extracting polysaccharide by orthogonal test design;

2. extracting at 60 deg.C for 60min for 3 times with a material-water ratio of 1:25g/ml and ultrasonic power of 300W;

3. centrifuging after extraction is finished, removing filter residues, concentrating the obtained filtrate on a rotary evaporator under reduced pressure, and removing protein by a savage method;

4. adding 95% ethanol to make ethanol content reach 80%, standing at low temperature overnight, centrifuging, washing the residue with diethyl ether and acetone solution for 6 times, drying to obtain gray powder extract, and determining polysaccharide content.

Example 3

1. Washing radix Angelicae sinensis powder with pure water, draining, and extracting polysaccharide by orthogonal test design;

2. extracting at 60 deg.C for 90min for 4 times with a material-water ratio of 1:30g/ml and ultrasonic power of 400W;

3. centrifuging after extraction is finished, removing filter residues, concentrating the obtained filtrate on a rotary evaporator under reduced pressure, and removing protein by a savage method;

4. adding 95% ethanol to make ethanol content reach 80%, standing at low temperature overnight, centrifuging, washing the residue with diethyl ether and acetone solution for 6 times, drying to obtain gray powder extract, and determining polysaccharide content.

Claims (4)

1. The extraction process of the angelica polysaccharide is characterized by comprising the following steps:

a. washing radix Angelicae sinensis powder with pure water, draining, and extracting polysaccharide by orthogonal test design;

b. extracting by taking the extraction temperature, the extraction time, the extraction times, the material-water ratio and the ultrasonic power as investigation factors;

c. centrifuging after extraction is finished, removing filter residues, concentrating the obtained filtrate on a rotary evaporator under reduced pressure, and removing protein by a savage method;

d. adding ethanol to make ethanol content reach 80%, standing at low temperature overnight, centrifuging, washing the residue with the solution for several times, drying to obtain gray powder extract, and determining polysaccharide content.

2. The process of claim 1, wherein the extraction temperature is 60 ℃, the extraction time is 75min, the number of extraction times is 2, the ratio of material to water is 1:35g/ml, and the super-success rate is 200W.

3. The method according to claim 1, wherein the concentration of the ethanol solution added in step d is 95%.

4. The solution used for washing in step d of claim 1 is diethyl ether or ethyl ketone, and the number of washing times is 6.