CN111212659A - Hpv疫苗 - Google Patents

Abstract

本发明涉及一种核酸，其编码包含多个保守肽序列或其变体的多肽，其中所述保守序列在一个或多个HPV基因型16、18、31、52、53和58中是保守的；并且其中所述多肽包含HPV蛋白质E1、E2、E4、E5、E6和E7中的每一种的保守肽序列；并且涉及相关的疫苗、病毒载体、治疗和预防。

Description

HPV疫苗

技术领域

本发明涉及用于对HPV感染疫苗接种的病毒载体疫苗。

背景技术

人乳头瘤病毒感染是一种由人乳头瘤病毒(HPV)引起的感染。大多数HPV感染不引起症状且自发解决。然而，在一些情况下，它们持续存在并且这可能导致出现疣或癌前病变。癌前病变增加子宫颈、阴门、阴道、阴茎、肛门、口腔或咽喉癌的风险。

全世界每年发生约50万例HPV引起的子宫颈癌，并且这些中的一半以上是致命的。大约85％的病例发生在低/中收入国家，在这些国家，可用治疗有限或没有可用治疗。未接受过预防性疫苗的女性需要每3年进行一次筛查以识别和治疗宫颈上皮内瘤变(CIN)。筛查每年花费英国国家医疗服务体系约1.75亿英镑。

针对CIN的当前疗法是通过电灼术或外科手术切除异常的宫颈组织。当前不存在可用于消除HPV病毒感染的疗法。女性在治疗之后需要多次随访以确保没有复发。疗法还与早产风险增加有关。

已经开发出用于现有HPV感染疗法的HPV疫苗包括Inovio-VGX-3 100(编码E6、E7的DNA)，类似于Genexine(GX-188E)；编码E2、E6、E7融合蛋白的Janssen-Ad26/Ad35+/-MVA；合成长肽(E6、E7)和类似物，例如PepCan，GTL001；Advaxis-ADXS-HPV；以及编码E7的单核细胞性李斯特菌。然而，这类开发出的疫苗仅针对HPV 16和18，具有安全性问题和/或功效低。例如，在IIb期随机对照试验中，VGX-3100的净功效是18％(疫苗组中的48％对安慰剂组中的30％)。

发明内容

所需要的是一种安全、易于递送并且具有比迄今测试的治疗性疫苗候选物更高的功效的疫苗。因此，本发明的目的是提供一种针对HPV感染的改善疫苗。

根据本发明的第一方面，提供了一种核酸，其编码包含多个保守肽序列或其变体的多肽，

其中所述保守序列在一个或多个HPV基因型16、18、31、52、53和58中是保守的；并且

其中所述多肽包含HPV蛋白质E1、E2、E4、E5、E6和E7中的每一种的保守肽序列。

本发明有利地提供了一种新型的替代且更安全的疫苗接种方法，由此可以将T细胞诱导至病毒体相对保守的抗原。使用来自非结构蛋白的专门设计的保守病毒区段可以提供针对多种重要基因型的保护。

在一个实施方式中，多肽是融合多肽。多肽可以不是野生型多肽。多肽可以是合成的/人工的，例如，多肽可能不在自然界中存在。在一个实施方式中，多肽可以不包含完整的基因序列。多肽可以基本上由保守肽序列组成。在另一个实施方式中，多肽可以基本上由保守肽序列和肽佐剂序列组成。在另一个实施方式中，多肽可以基本上由保守肽序列和其间的一个或多个接头组成。在另一个实施方式中，多肽可以基本上由保守肽序列、肽佐剂序列和其间的一个或多个接头组成。在一个实施方式中，多肽是重组多肽，诸如重组融合多肽。

本文所用的术语“融合多肽”应理解为意指包含衍生自不同基因产物(例如不同的HPV蛋白)的序列的组合或来自相同基因产物(例如单一HPV蛋白)的序列的组合的多肽，其中这些序列来自野生型基因产物的不同/单独的区域。例如，融合多肽可以包含通常被野生型中的其它序列区段分开的序列的组合，并且已经移除了一个或多个分离序列。

本文所用的术语“保守肽序列”或“保守区段”定义为在一个或多个基因型中保守的序列，如下文所定义。在评估保守性之前，从NCBI蛋白质数据库(2014年访问)中收集了来自基因型16、18、31、52、53和58的HPV蛋白E1、E2、E4、E5、E6和E7的所有可用全长序列并且用作本发明方法的输入。使用所有可用序列以确保所选的保守肽序列将等效地代表整个环境种群(参见表1)。使用‘变体’方法识别(图1A)保守肽序列；在保守评估之前，将所有基因型比对并且加权每个基因型内的序列以确保基因型多样性的等效代表并因此确保疫苗候选物代表整个环境种群。然后使用15个氨基酸的滑动窗口评估基因型内的保守性(基因型内保守性)，从而根据组合窗口内的氨基酸普遍性和对每个序列的值加权来确定每个窗口的保守值，以识别在每个基因型内保守的片段，以及针对每个窗口创建的标准化基因型内共有序列。‘标准化共有序列’意指代表基因型序列的加权集合的氨基酸序列，而不是每个位置上最常见的氨基酸。要被分类为保守，该窗口必须在蛋白质的所有窗口保守值的第一个四分位数内具有保守值。随后，识别所有基因型中相同位置处的保守基因型内窗口，而与基因型之间共享的基因型内标准化共有序列的同一性百分比无关(基因型间保守)。然后创建所得区域的系统发育并且组合树内群序列以产生具有高水平共享的共有同一性的基因型间共有序列。在此情况下，“基因型间共有序列”是指使用从每个基因型创建的标准化共有序列创建的共有序列。在某些情况下，创建‘经修饰变体’，其中识别了在所有蛋白质中处于相同位置的保守基因型内窗口，这些基因型内窗口在基因型之间共有大于60％共享的基因型内标准化共有序列同一性百分比。

如果从树内群中识别出的血清型间片段具有小于60％的同一性百分比，则这些序列被分类为“高度差异的”，与变体方法中使用的“差异较小”序列形成对比。对于基因型之间高度差异的蛋白质，使用“嵌合”方法识别保守肽序列(图1B)；建立系统发育树且仅将基因型内群在一起比对，并且评估基因型内保守性。因此，仅评估内群之间的基因型间保守性并且选择基因型中具有大于60％共享的共有同一性的血清型内保守窗口。在一些情况下，基因型作为‘嵌合体’运行，但未评估基因间保守性(‘嵌合变体’)。

所述多个保守肽序列可以包含10个或更多个保守肽序列。在另一个实施方式中，所述多个保守肽序列可以包含15个或更多个保守肽序列。在另一个实施方式中，所述多个保守肽序列可以包含20个或更多个保守肽序列。在另一个实施方式中，所述多个保守肽序列可以包含25个或更多个保守肽序列。在另一个实施方式中，所述多个保守肽序列可以包含30个或更多个保守肽序列。所述多个保守序列可以包含35个或更多个保守肽序列。在另一个实施方式中，所述多个保守肽序列可以包含40个或更多个保守肽序列。所述多个保守肽序列可以包含45个或更多个保守肽序列。在另一个实施方式中，所述多个保守肽序列可以包含50个或更多个保守肽序列。在另一个实施方式中，所述多个保守肽序列可以包含55个或更多个保守肽序列。在一个实施方式中，所述多个保守肽序列由约56个或更多个保守肽序列组成。在一个实施方式中，所述多个保守肽序列由约57个或更多个保守肽序列组成。在一个实施方式中，所述多个保守肽序列由约58个或更多个保守肽序列组成。在一个实施方式中，所述多个保守肽序列由约59个或更多个保守肽序列组成。在一个实施方式中，所述多个保守肽序列由约59个保守肽序列组成。

经编码的多肽可以包含HPV蛋白质E1、E2、E4、E5、E6和E7中的每一种的至少3个不同的保守肽序列。经编码的多肽可以包含HPV蛋白质E1的至少3个不同的保守肽序列。经编码的多肽可以包含HPV蛋白质E2的至少3个不同的保守肽序列。经编码的多肽可以包含HPV蛋白质E4的至少3个不同的保守肽序列。经编码的多肽可以包含HPV蛋白质E5的至少3个不同的保守肽序列。经编码的多肽可以包含HPV蛋白质E6的至少3个不同的保守肽序列。经编码的多肽可以包含HPV蛋白质E7的至少3个不同的保守肽序列。

经编码的多肽可以包含HPV蛋白质E1的至少4个不同的保守肽序列。经编码的多肽可以包含HPV蛋白质E2的至少4个不同的保守肽序列。经编码的多肽可以包含HPV蛋白质E4的至少4个不同的保守肽序列。经编码的多肽可以包含HPV蛋白质E6的至少4个不同的保守肽序列。经编码的多肽可以包含HPV蛋白质E7的至少4个不同的保守肽序列。

经编码的多肽可以包含HPV蛋白质E1的至少5个不同的保守肽序列。经编码的多肽可以包含HPV蛋白质E2的至少5个不同的保守肽序列。经编码的多肽可以包含HPV蛋白质E4的至少5个不同的保守肽序列。经编码的多肽可以包含HPV蛋白质E6的至少5个不同的保守肽序列。

经编码的多肽可以包含HPV蛋白质E1的至少6个不同的保守肽序列。经编码的多肽可以包含HPV蛋白质E2的至少6个不同的保守肽序列。经编码的多肽可以包含HPV蛋白质E4的至少6个不同的保守肽序列。经编码的多肽可以包含HPV蛋白质E6的至少6个不同的保守肽序列。

经编码的多肽可以包含HPV蛋白质E1的至少7个不同的保守肽序列。经编码的多肽可以包含HPV蛋白质E2的至少7个不同的保守肽序列。经编码的多肽可以包含HPV蛋白质E4的至少7个不同的保守肽序列。经编码的多肽可以包含HPV蛋白质E6的至少7个不同的保守肽序列。

经编码的多肽可以包含HPV蛋白质E1的至少8个不同的保守肽序列。经编码的多肽可以包含HPV蛋白质E2的至少8个不同的保守肽序列。经编码的多肽可以包含HPV蛋白质E4的至少8个不同的保守肽序列。经编码的多肽可以包含HPV蛋白质E6的至少8个不同的保守肽序列。

经编码的多肽可以包含HPV蛋白质E1的至少9个不同的保守肽序列。经编码的多肽可以包含HPV蛋白质E2的至少9个不同的保守肽序列。经编码的多肽可以包含HPV蛋白质E4的至少9个不同的保守肽序列。

经编码的多肽可以包含HPV蛋白质E1的至少10个不同的保守肽序列。经编码的多肽可以包含HPV蛋白质E2的至少10个不同的保守肽序列。

经编码的多肽可以包含HPV蛋白质E1的至少11个不同的保守肽序列。经编码的多肽可以包含HPV蛋白质E2的至少11个不同的保守肽序列。

经编码的多肽可以包含HPV蛋白质E2的至少12、13、14、15、16、17、18、19、20、21、22、23、或24个不同的保守肽序列。

经编码的多肽可以包含以下或由其组成：HPV蛋白质E1的11个或更多个不同的保守肽序列、HPV蛋白质E2的24个或更多个不同的保守肽序列、HPV蛋白质E4的9个或更多个不同的保守肽序列、HPV蛋白质E5的3个或更多个不同的保守肽序列、HPV蛋白质E6的8个或更多个不同的保守肽序列、以及HPV蛋白质E7的4个或更多个不同的保守肽序列。

所述多个保守肽序列可以衍生自相对于彼此不同的序列区域(即非天然同时存在的)。例如，提及“不同的保守肽序列”可以包含衍生自相对于彼此不同的野生型序列区域(即非天然同时存在的)。例如，在野生型基因型中，保守序列在野生型基因型中可以由可变/非保守序列分开。所述多个保守肽序列可以彼此不重叠或不显著重叠。所述多个保守肽序列中的两个或更多个或全部可以在多肽中直接连接在一起，例如其间不包含任何非保守/可变残基。多肽序列可能在自然界中找不到。多肽可以不包含非保守序列或残基。保守肽序列在多肽序列中不会间隔超过1、2、3、4或5个残基，例如在其中保守肽序列之间存在接头/连接残基的实施方式中。可替代地，保守肽序列在多肽序列中不会间隔超过6、7、8、9或10个残基，例如在其中保守肽序列之间存在接头/连接残基的实施方式中。多肽可以不包含长于2、3、4、5、6、7、8、9或10个氨基酸的非保守序列。

在一个实施方式中，可以在一个或多个、或所有保守肽序列之间提供接头残基(例如在多肽中的保守肽序列之间提供连接)。接头残基可以包含随机氨基酸序列，或基于表位预测计算机程序或动物模型中的实验选择为非免疫原性的氨基酸。例如，如果预测或已知其为表位(即为了避免对在HPV中未发现的表位(例如人工表位)免疫应答)，则不将其视为接头。接头可以是柔性的。接头可以包含K、G、P、A或S氨基酸残基或其组合或由其组成。在一个实施方式中，接头可以包含G和/或P氨基酸残基或由其组成。在一个实施方式中，接头可以包含一个或多个丙氨酸(A)氨基酸残基或由其组成。接头残基的长度可以在1与10个氨基酸之间。在另一个实施方式中，接头残基的长度可以在2与8个残基之间。在另一个实施方式中，接头残基的长度可以在1与6个残基之间。保守肽序列可以在多肽序列中间隔1至10个残基，例如在其中保守肽序列之间存在接头/连接残基的实施方式中。

在一个实施方式中，多肽可以基本上由保守肽序列和一个或多个接头组成，任选地其中一个或多个接头置于相邻的保守肽序列之间。

保守肽序列可以选自包含SEQ ID NO:1至59；其变体或其组合的组中的任一种。在另一个实施方式中，保守肽序列可以选自以任何顺序包含SEQ ID NO:1至59；其变体或其组合的组中的任一种。在一个实施方式中，保守肽序列可以由包含SEQ ID NO:1至59的组组成。

多肽可以包含选自SEQ ID NO:1-11中的任一种的一个或多个保守E1序列；选自SEQ ID NO:12-35中的任一种的一个或多个保守E2序列；选自SEQ ID NO:36-44中的任一种的一个或多个保守E4序列；选自SEQ ID NO:45-47中的任一种的一个或多个保守E5序列；选自SEQ ID NO:48-55中的任一种的一个或多个保守E6序列；以及选自SEQ ID NO:56-59中的任一种的一个或多个保守E7序列。

多肽可以包含选自SEQ ID NO:1-11中的任一种的两个或更多个保守E1序列；选自SEQ ID NO:12-35中的任一种的两个或更多个保守E2序列；选自SEQ ID NO:36-44中的任一种的两个或更多个保守E4序列；选自SEQ ID NO:45-47中的任一种的两个或更多个保守E5序列；选自SEQ ID NO:48-55中的任一种的两个或更多个保守E6序列；以及选自SEQ ID NO:56-59中的任一种的两个或更多个保守E7序列。

多肽可以包含选自SEQ ID NO:1-11中的任一种的三个或更多个保守E1序列；选自SEQ ID NO:12-35中的任一种的三个或更多个保守E2序列；选自SEQ ID NO:36-44中的任一种的三个或更多个保守E4序列；选自SEQ ID NO:45-47中的任一种的三个或更多个保守E5序列；选自SEQ ID NO:48-55中的任一种的三个或更多个保守E6序列；以及选自SEQ ID NO:56-59中的任一种的三个或更多个保守E7序列。

保守序列在HPV基因型16、18、31、52和58中的一个或多个中是保守的。保守序列在所有HPV基因型16、18、31、52和58中是保守的。

多肽可以包含：

选自SEQ ID NO:1-11中的任一种的一个或多个保守E1序列，其中所述基因型16、18、31、52、53和58中的每一种由至少一个保守E1序列表示；

选自SEQ ID NO:12-35中的任一种的一个或多个保守E2序列，其中所述基因型16、18、31、52、53和58中的每一种由至少一个保守E2序列表示；

选自SEQ ID NO:36-44中的任一种的一个或多个保守E4序列，其中所述基因型16、18、31、52、53和58中的每一种由至少一个保守E4序列表示；

选自SEQ ID NO:45-47中的任一种的一个或多个保守E5序列，其中所述基因型16、18、31、52、53和58中的每一种由至少一个保守E5序列表示；

选自SEQ ID NO:48-55中的任一种的一个或多个保守E6序列，其中所述基因型16、18、31、52、53和58中的每一种由至少一个保守E6序列表示；和

选自SEQ ID NO:56-59中的任一种的一个或多个保守E7序列，其中所述基因型16、18、31、52、53和58中的每一种由至少一个保守E7序列表示。

多肽可以包含：

选自SEQ ID NO:1-11中的任一种的一个或多个保守E1序列，其中所述基因型16、18、31、52和58中的每一种由至少一个保守E1序列表示；

选自SEQ ID NO:12-35中的任一种的一个或多个保守E2序列，其中所述基因型16、18、31、52和58中的每一种由至少一个保守E2序列表示；

选自SEQ ID NO:36-44中的任一种的一个或多个保守E4序列，其中所述基因型16、18、31、52和58中的每一种由至少一个保守E4序列表示；

选自SEQ ID NO:45-47中的任一种的一个或多个保守E5序列，其中所述基因型16、18、31、52和58中的每一种由至少一个保守E5序列表示；

选自SEQ ID NO:48-55中的任一种的一个或多个保守E6序列，其中所述基因型16、18、31、52和58中的每一种由至少一个保守E6序列表示；和

选自SEQ ID NO:56-59中的任一种的一个或多个保守E7序列，其中所述基因型16、18、31、52和58中的每一种由至少一个保守E7序列表示。

多肽可以包含：

选自SEQ ID NO:1-11中的任一种的两个或更多个保守E1序列，其中所述基因型16、18、31、52、53和58中的每一种在保守E1序列的组中表示；

选自SEQ ID NO:12-35中的任一种的两个或更多个保守E2序列，其中所述基因型16、18、31、52、53和58中的每一种在保守E2序列的组中表示；

选自SEQ ID NO:36-44中的任一种的两个或更多个保守E4序列，其中所述基因型16、18、31、52、53和58中的每一种在保守E4序列的组中表示；

选自SEQ ID NO:45-47中的任一种的两个或更多个保守E5序列，其中所述基因型16、18、31、52、53和58中的每一种在保守E5序列的组中表示；

选自SEQ ID NO:48-55中的任一种的两个或更多个保守E6序列，其中所述基因型16、18、31、52、53和58中的每一种在保守E6序列的组中表示；和

选自SEQ ID NO:56-59中的任一种的两个或更多个保守E7序列，其中所述基因型16、18、31、52、53和58中的每一种在保守E7序列的组中表示。

多肽可以包含：

选自SEQ ID NO:1-11中的任一种的两个或更多个保守E1序列，其中所述基因型16、18、31、52和58中的每一种在保守E1序列的组中表示；

选自SEQ ID NO:12-35中的任一种的两个或更多个保守E2序列，其中所述基因型16、18、31、52和58中的每一种在保守E2序列的组中表示；

选自SEQ ID NO:36-44中的任一种的两个或更多个保守E4序列，其中所述基因型16、18、31、52和58中的每一种在保守E4序列的组中表示；

选自SEQ ID NO:45-47中的任一种的两个或更多个保守E5序列，其中所述基因型16、18、31、52和58中的每一种在保守E5序列的组中表示；

选自SEQ ID NO:48-55中的任一种的两个或更多个保守E6序列，其中所述基因型16、18、31、52和58中的每一种在保守E6序列的组中表示；和

选自SEQ ID NO:56-59中的任一种的两个或更多个保守E7序列，其中所述基因型16、18、31、52和58中的每一种在保守E7序列的组中表示。

多肽可以包含：

选自SEQ ID NO:1-11中的任一种的三个或更多个保守E1序列，其中所述基因型16、18、31、52、53和58中的每一种在保守E1序列的组中表示；

选自SEQ ID NO:12-35中的任一种的三个或更多个保守E2序列，其中所述基因型16、18、31、52、53和58中的每一种在保守E2序列的组中表示；

选自SEQ ID NO:36-44中的任一种的三个或更多个保守E4序列，其中所述基因型16、18、31、52、53和58中的每一种在保守E4序列的组中表示；

选自SEQ ID NO:45-47的三个保守E5序列，其中所述基因型16、18、31、52、53和58中的每一种在保守E5序列的组中表示；

选自SEQ ID NO:48-55中的任一种的三个或更多个保守E6序列，其中所述基因型16、18、31、52、53和58中的每一种在保守E6序列的组中表示；和

选自SEQ ID NO:56-59中的任一种的三个或更多个保守E7序列，其中所述基因型16、18、31、52、53和58中的每一种在保守E7序列的组中表示。

多肽可以包含：

选自SEQ ID NO:1-11中的任一种的三个或更多个保守E1序列，其中所述基因型16、18、31、52和58中的每一种在保守E1序列的组中表示；

选自SEQ ID NO:12-35中的任一种的三个或更多个保守E2序列，其中所述基因型16、18、31、52和58中的每一种在保守E2序列的组中表示；

选自SEQ ID NO:36-44中的任一种的三个或更多个保守E4序列，其中所述基因型16、18、31、52和58中的每一种在保守E4序列的组中表示；

选自SEQ ID NO:45-47的三个保守E5序列，其中所述基因型16、18、31、52和58中的每一种在保守E5序列的组中表示；

选自SEQ ID NO:48-55中的任一种的三个或更多个保守E6序列，其中所述基因型16、18、31、52和58中的每一种在保守E6序列的组中表示；和

选自SEQ ID NO:56-59中的任一种的三个或更多个保守E7序列，其中所述基因型16、18、31、52和58中的每一种在保守E7序列的组中表示；

提及“表示基因型16、18、31、52、53和58中的每一种”或“表示基因型16、18、31、52和58中的每一种”旨在意指每个已识别的基因型已用于定义保守肽序列的至少一个共有序列。因此，给定组可以包含来自每个基因型的保守肽，或者保守肽可以衍生自两个或更多个基因型的共有序列。如果序列同一性足够相似，则所有基因型16、18、31、52、53和58或16、18、31、52和58可以由单一保守肽序列表示，该保守肽序列可以分别是所有基因型16、18、31、52、53和58或16、18、31、52和58的共有序列。然而，由于序列同一性的差异，单一保守肽可能无法表示来自所有基因型16、18、31、52、53和58或16、18、31、52和58的共有序列并且取代需要两个或更多个保守肽序列来覆盖/表示所有基因型16、18、31、52、53和58或16、18、31、52和58。例如(仅出于说明目的)，一个保守E6肽序列可以表示E6基因型16和18，另一个可以表示E6基因型52，并且第三个可以表示E6基因型53和58，使得组中的全部三个保守E6肽序列表示全部E6基因型16、18、31、52、53和58或16、18、31、52和58。

核酸可以包含序列SEQ ID NO:60或其变体或由其组成。在另一个实施方式中，核酸可以包含序列SEQ ID NO:60或其变体或由其组成，并且不编码TPA前导序列。在另一个实施方式中，核酸可以包含序列SEQ ID NO:60或其变体或由其组成，编码不同/替代的肽佐剂而非TPA前导序列。在另一个实施方式中，核酸可以包含序列SEQ ID NO:65或其变体或由其组成。

核酸的变体可以包含与SEQ ID NO:60或65具有至少80％同一性的序列或由其组成。可替代地，核酸的变体可以包含与SEQ ID NO:60或65具有至少85％同一性的序列或由其组成。核酸的变体可以包含与SEQ ID NO:60或65具有至少90％同一性的序列或由其组成。核酸的变体可以包含与SEQ ID NO:60或65具有至少95％同一性的序列或由其组成。核酸的变体可以包含与SEQ ID NO:60或65具有至少98％同一性的序列或由其组成。核酸的变体可以包含与SEQ ID NO:60或65具有至少99％同一性的序列或由其组成。熟练人员将理解，核酸的变体可以包括编码与SEQ ID NO:60或65相同的肽的冗余密码子变体。

核酸可以包含序列SEQ ID NO:62或其变体或由其组成。在另一个实施方式中，核酸可以包含序列SEQ ID NO:62或其变体或由其组成，并且不编码TPA前导序列。在另一个实施方式中，核酸可以包含序列SEQ ID NO:62或其变体或由其组成，编码不同/替代的肽佐剂而非TPA前导序列。

核酸的变体可以包含与SEQ ID NO:62具有至少80％同一性的序列或由其组成。可替代地，核酸的变体可以包含与SEQ ID NO:62具有至少85％同一性的序列或由其组成。核酸的变体可以包含与SEQ ID NO:62具有至少90％同一性的序列或由其组成。核酸的变体可以包含与SEQ ID NO:62具有至少95％同一性的序列或由其组成。核酸的变体可以包含与SEQ ID NO:62具有至少98％同一性的序列或由其组成。核酸的变体可以包含与SEQ ID NO:62具有至少99％同一性的序列或由其组成。核酸的变体可以包含与SEQ ID NO:62具有至少99.5％同一性的序列或由其组成。核酸的变体可以包含与SEQ ID NO:62具有至少99.9％同一性的序列或由其组成。熟练人员将理解，核酸的变体可以包括编码与SEQ ID NO:62相同的病毒载体和/或肽的冗余密码子变体。

核酸可以包含序列SEQ ID NO:71、73或75或其变体或由其组成。在另一个实施方式中，核酸可以包含序列SEQ ID NO:71、73或75或其变体或由其组成，并且不编码TPA前导序列。在另一个实施方式中，核酸可以包含序列SEQ ID NO:71、73或75或其变体或由其组成，编码不同/替代的肽佐剂而非TPA前导序列。

核酸的变体可以包含与SEQ ID NO:71、73或75具有至少80％同一性的序列或由其组成。可替代地，核酸的变体可以包含与SEQ ID NO:71、73或75具有至少85％同一性的序列或由其组成。核酸的变体可以包含与SEQ ID NO:71、73或75具有至少90％同一性的序列或由其组成。核酸的变体可以包含与SEQ ID NO:71、73或75具有至少95％同一性的序列或由其组成。核酸的变体可以包含与SEQ ID NO:71、73或75具有至少98％同一性的序列或由其组成。核酸的变体可以包含与SEQ ID NO:71、73或75具有至少99％同一性的序列或由其组成。核酸的变体可以包含与SEQ ID NO:71、73或75具有至少99.5％同一性的序列或由其组成。核酸的变体可以包含与SEQ ID NO:71、73或75具有至少99.9％同一性的序列或由其组成。熟练人员将理解，核酸的变体可以包括编码与SEQ ID NO:71、73或75相同的病毒载体和/或肽的冗余密码子变体。

多肽可以包含序列SEQ ID NO:61或其变体或由其组成。在另一个实施方式中，多肽可以包含序列SEQ ID NO:61或其变体或由其组成，并且不编码TPA前导序列。在另一个实施方式中，多肽可以包含序列SEQ ID NO:61或其变体或由其组成，具有不同/替代的肽佐剂而非TPA前导序列。在另一个实施方式中，多肽可以包含序列SEQ ID NO:66或其变体或由其组成。

多肽可以包含序列SEQ ID NO:72、74或76或其变体或由其组成。在另一个实施方式中，多肽可以包含序列SEQ ID NO:72、74或76或其变体或由其组成，并且不编码TPA前导序列。在另一个实施方式中，多肽可以包含序列SEQ ID NO:72、74或76或其变体或由其组成，具有不同/替代的肽佐剂而非TPA前导序列。在另一个实施方式中，多肽可以包含序列SEQ ID NO:72、74或76或其变体或由其组成。

在一个实施方式中，多肽可以基本上由保守肽序列和肽佐剂组成。在一个实施方式中，多肽可以基本上由保守肽序列、一个或多个接头和肽佐剂组成。一个或多个接头可置于相邻的保守肽序列之间。肽佐剂可以是N-末端。

多肽的变体可以包含与SEQ ID NO:61、66、72、74或76具有至少80％同一性的序列或由其组成。可替代地，多肽的变体可以包含与SEQ ID NO:61、66、72、74或76具有至少85％同一性的序列或由其组成。多肽的变体可以包含与SEQ ID NO:61、66、72、74或76具有至少90％同一性的序列或由其组成。多肽的变体可以包含与SEQ ID NO:61、66、72、74或76具有至少95％同一性的序列或由其组成。多肽的变体可以包含与SEQ ID NO:61、66、72、74或76具有至少98％同一性的序列或由其组成。多肽的变体可以包含与SEQ ID NO:61、66、72、74或76具有至少99％同一性的序列或由其组成。

保守肽序列的变体可以包含保守肽序列的截短序列或由其组成。例如，本文的SEQID NO:1至59的任一个或多个序列可以被截短并且仍在多肽中提供免疫原性。截短序列可以包含足够数量的氨基酸以从SEQ ID NO:1至59的任一个序列内的序列形成可识别的表位(例如对于特异性T细胞识别至少最小数量的残基)。截短序列可以包含SEQ ID NO:1至59的序列的至少7个氨基酸。可替代地，截短序列可以包含SEQ ID NO:1至59的序列的至少8个氨基酸。可替代地，截短序列可以包含SEQ ID NO:1至59的序列的至少9、10、11或12个氨基酸。可以在SEQ ID NO:1至59的保守肽序列之一内提供多个截短序列。

在一个实施方式中，SEQ ID NO:1至59的任一个保守肽序列可以例如通过残基取代、添加或缺失而变化。在另一个实施方式中，SEQ ID NO:1至59的一些或全部保守肽序列可以例如通过残基取代、添加或缺失而变化。变体保守肽序列仍可起作用以提供可识别的HPV表位。熟练人员将理解，任何给定的群体中都存在天然变异，并且这些变异可以具有共有序列或SEQ ID NO:1至59中提供的示例患者序列的某些序列变异。因此，变体保守肽序列可以与SEQ ID NO:1至59中的任一个具有至少70％序列同一性。在另一个实施方式中，变体保守肽序列可以与SEQ ID NO:1至59中的任一个具有至少74％序列同一性。在另一个实施方式中，变体保守肽序列可以与SEQ ID NO:1至59中的任一个具有至少75％序列同一性。在另一个实施方式中，变体保守肽序列可以与SEQ ID NO:1至59中的任一个具有至少79％序列同一性。在另一个实施方式中，变体保守肽序列可以与SEQ ID NO:1至59中的任一个具有至少80％序列同一性。在另一个实施方式中，变体保守肽序列可以与SEQ ID NO:1至59中的任一个具有至少82％序列同一性。在另一个实施方式中，变体保守肽序列可以与SEQ IDNO:1至59中的任一个具有至少83％序列同一性。在另一个实施方式中，变体保守肽序列可以与SEQ ID NO:1至59中的任一个具有至少85％序列同一性。在另一个实施方式中，变体保守肽序列可以与SEQ ID NO:1至59中的任一个具有至少88％序列同一性。在另一个实施方式中，变体保守肽序列可以与SEQ ID NO:1至59中的任一个具有至少90％序列同一性。在另一个实施方式中，变体保守肽序列可以与SEQ ID NO:1至59中的任一个具有至少92％序列同一性。在另一个实施方式中，变体保守肽序列可以与SEQ ID NO:1至59中的任一个具有至少95％序列同一性。在另一个实施方式中，变体保守肽序列可以与SEQ ID NO:1至59中的任一个具有至少98％序列同一性。在另一个实施方式中，变体保守肽序列可以与SEQ ID NO:1至59中的任一个具有至少99％序列同一性。在另一个实施方式中，变体保守肽序列可以与SEQ ID NO:1至59中的任一个具有至少99.5％序列同一性。

提及本文所用的序列“同一性”可以指使用标准NCBI BLASTp参数的两个比对序列之间的同一性百分比(http://blast.ncbi.nlm.nih.gov)。

保守肽序列的长度可以变化，最小长度定义为形成可识别表位所需的最小残基数。因此，保守肽序列的长度可以是约7至250个氨基酸或更多。例如，至少一个保守肽序列的长度可以是至少约7个氨基酸。在另一个实施方式中，至少一个保守肽序列的长度可以是至少约8个氨基酸。在另一个实施方式中，至少一个保守肽序列的长度可以是至少约10个氨基酸。在另一个实施方式中，至少一个保守肽序列的长度可以是至少约15个氨基酸。在另一个实施方式中，至少一个保守肽序列的长度可以是至少约20个氨基酸。在另一个实施方式中，至少一个保守肽序列的长度可以是至少约30个氨基酸。在一个实施方式中，至少一个保守肽序列的长度可以在约20与约220个氨基酸之间。在一个实施方式中，至少一个保守肽序列的长度可以不超过约300个氨基酸。在另一个实施方式中，至少一个保守肽序列的长度可以不超过约250个氨基酸。在另一个实施方式中，至少一个保守肽序列的长度可以不超过约200个氨基酸。在另一个实施方式中，至少一个保守肽序列的长度可以不超过约150个氨基酸。在另一个实施方式中，至少一个保守肽序列的长度可以不超过约100个氨基酸。在另一个实施方式中，至少一个保守肽序列的长度可以不超过约55个氨基酸。在另一个实施方式中，至少一个保守肽序列的长度可以不超过约54个氨基酸。

保守肽序列可以是保守肽序列群中在约15与约50个氨基酸之间的平均长度。

在本发明的一些实施方式中，多肽可以进一步包含肽佐剂，诸如TPA(组织型纤溶酶原激活物)序列或其功能变体。TPA可以包含以下序列或由其组成：MDAMKRGLCCVLLLCGAVFVSPSQEIHARFRR(SEQ ID NO:63)或其功能变体。在一个实施方式中，肽佐剂可以包含例如序列SLLWGGVTVLAAMLIAGQVASSVVFLV(SEQ ID NO:64)或其功能变体的Shark不变链。肽佐剂可以是本发明的多肽上的N-末端。肽佐剂的功能变体可以是截短或突变肽变体，其仍可以充当佐剂，例如仍充当佐剂的TPA或shark不变链的截短或突变变体。熟练人员将了解，可以在不影响功能的情况下取代、添加或去除1、2、3、4、5或更多个氨基酸残基。例如，可以考虑保守取代。在提供(或适当时编码)肽佐剂的实施方式中，可以另外提供在肽佐剂与第一保守肽序列之间提供(或编码)的接头序列。在没有肽佐剂的实施方式中，可以不提供第一接头序列。

核酸的组合可以编码根据本发明的不同多肽，可以作为疫苗提供。例如，初免和/或加强疫苗制剂可以包含编码两种或更多种本发明的多肽的核酸或病毒载体，所述多肽可以相对于彼此不同。

核酸可以与另一种治疗或预防活性成分组合用于疫苗。核酸可以与佐剂组合用于疫苗。

根据本发明的另一方面，提供了一种包含多种根据本发明的不同核酸的组合物，任选地其中该组合物是药学上可接受的组合物。

根据本发明的另一方面，本文提供了一种由根据本发明的核酸编码的多肽。

在一个实施方式中，多肽是分离多肽。多肽、编码该多肽的核酸或相关的病毒颗粒可以在药学上可接受的载体中提供。

核酸可以是用于疫苗接种的质粒载体。核酸可以包含病毒载体序列。

根据本发明的另一方面，本文提供了一种病毒载体，该病毒载体包含根据本发明的核酸。

病毒载体可以包含病毒。病毒载体可以包含腺病毒，诸如人或猿猴腺病毒。当用于初免加强方案的初免疫苗中时，病毒载体可以包含腺病毒。病毒载体可以包含ChAdOx1(E群猿猴腺病毒，如在疟疾试验中安全使用的AdCh63载体)或ChAdOx2(如Morris等人2016.Future Virol 11(9),第649-659页中所述)。ChAdOx2序列可以包含本文所述的序列(例如SEQ ID NO:67+68)或由其组成。病毒载体可以包含AdCh63。病毒载体可以包含AdC3或AdH6。病毒载体可以是人血清型。病毒载体可以包含修饰的痘苗安卡拉(MVA)。病毒载体可以包含F11 MVA(例如在F11基因座处具有核酸构建体插入物的MVA)。本发明的核酸(HPV疫苗构建体插入物)可以在p7.5启动子的控制下，例如通过与p7.5 MVA穿梭质粒(SEQ ID NO:158)重组在亲本MVA病毒的TK基因座处插入。核酸可以包含序列SEQ ID NO：158，该序列具有如SEQ ID NO:158中提供的核酸疫苗构建体插入物(加下划线)，或具有本文根据本发明的替代核酸疫苗构建体。在另一个实施方式中，本发明的核酸(HPV疫苗构建体插入物)可以在F11启动子的控制下，例如通过与F11穿梭质粒(SEQ ID NO:159)重组在亲本MVA病毒的F11基因座处插入。核酸可以包含序列SEQ ID NO：159，该序列具有如SEQ ID NO:159中提供的核酸疫苗构建体插入物(加下划线)，或具有本文根据本发明的替代核酸疫苗构建体。MVA序列可以包含本文所述的序列(例如SEQ ID NO:69++70)或由其组成。当用作初免加强方案中的疫苗加强时，病毒载体可以包含MVA。病毒载体可以包含腺相关病毒(AAV)或慢病毒。病毒载体可以是减毒的病毒载体。可以将本发明的多肽序列克隆到已知引起良好免疫应答的任何合适的病毒载体中。合适的病毒载体已经描述于Dicks等人(Vaccine.2015年2月25日；33(9):1121-8.doi:10.1016/j.vaccine.2015.01.042.Epub 2015年1月25日)、Antrobus等人(Mol Ther.2014年3月；22(3):668-74.doi:10.1038/mt.2013.284.Epub 2013年12月30日)，和Warimwe等人(Virol J.2013年12月5日；10:349.doi:10.1186/1743-422X-10-349)，其通过引用并入本文。

根据本发明的另一方面，提供了一种组合物，该组合物包含以下中的一种或多种：

-根据本发明的多肽；

-根据本发明的核酸；和

-根据本发明的病毒载体。

该组合物例如在哺乳动物诸如人中可以是免疫原性的。该组合物可以进一步包含药学上可接受的载体。该组合物可以是包含药学上可接受的载体的药物组合物。该组合物可以用于预防或治疗HPV感染。

根据本发明的另一方面，提供了一种治疗或预防HPV感染的方法，该方法包括施用：

-根据本发明的多肽；

-根据本发明的核酸；

-根据本发明的组合物；或

-根据本发明的病毒载体。

治疗或预防HPV感染的方法可以是疫苗接种方法。

根据本发明的另一方面，提供了一种用于预防或治疗HPV感染的试剂，该试剂包含以下或由其组成：

-根据本发明的多肽；

-根据本发明的组合物；

-根据本发明的核酸；或

-根据本发明的病毒载体。

在一个实施方式中，治疗或预防HPV感染包括治疗或预防肛门生殖器HPV驱动的病变，诸如肛门、阴门、阴道或阴茎上皮内瘤变。另外或可替代地，治疗或预防HPV感染包括治疗或预防由HPV引起的口咽部病变。

根据本发明的另一方面，提供了根据本发明的多肽；根据本发明的组合物；根据本发明的核酸；或根据本发明的病毒载体；其用于疫苗或用作疫苗。

根据本发明的另一方面，提供了一种疫苗，该疫苗包含本发明的核酸；根据本发明的多肽；根据本发明的组合物；或根据本发明的病毒载体。

疫苗可以是初免疫苗。疫苗可以是加强疫苗。在初免疫苗后提供加强疫苗的情况下，多肽可以不同。例如，多肽可以包含保守肽序列的重排序列。保守肽序列可以是相同的，但是它们在多肽中提供的顺序可以改变。因此，本文的发明提供其中可以改变提供保守肽序列的顺序的本发明的任何序列/实施方式。这类实施方式还可以包括重排或不同的接头/连接序列。

有利地，在初免疫苗与加强疫苗之间多肽的保守肽序列的重排可以避免提供在一个保守肽序列与另一个保守肽序列的连接处形成的“假”表位，即相同的连接可能不会在重排的多肽中出现。

根据本发明的另一方面，提供了根据本发明的核酸或多肽用于疫苗或用作疫苗。

根据本发明的另一方面，提供了一种初免加强疫苗接种试剂盒，其包括：

-根据本发明的初免疫苗接种；

-根据本发明的加强疫苗接种；

初免和加强疫苗接种可以不同。初免和加强疫苗接种的多肽序列可能不同。初免和加强疫苗接种可以包含不同的病毒载体(即来自不同病毒科，诸如MVA与腺病毒)。

根据本发明的另一方面，本文提供了一种组合物，该组合物包含根据本发明的核酸和药学上可接受的载体。

该组合物可以不包含野生型HPV。该组合物可以不包含全长HPV蛋白序列。病毒载体或核酸可能不编码HPV的非保守蛋白/肽序列。

可以与药学上可接受的载体一起使用。另外或可替代地，可以与佐剂一起使用。

当应用于本发明的核酸、多肽或组合物时，术语“免疫原性”意指能够在人或动物体内引发免疫应答。免疫应答可以是保护性的。

术语“保护性”意指预防疾病、疾病感染、传播和/或进展的风险降低、疾病的严重程度降低、治愈病状或疾病、症状缓解、或者疾病或疾病症状的严重程度降低。

术语“预防”意指对疾病的预防或保护性治疗。预防可以包括降低疾病感染、传播和/或进展的风险，或降低疾病的严重程度。

术语“治疗”意指治愈病状或疾病、减轻症状、或降低疾病或疾病症状的严重程度。

熟练人员将理解，本发明的一个实施方式或方面的任选特征在合适时可以适用于本发明的其它实施方式或方面。

附图说明

现在将仅通过例示的方式参考以下附图更详细地描述本发明的实施方式。

图1-用于创建HPV候选物的嵌合和变体方法。

图2-保守区及其下文的所得变体。

图3-A)被识别为在用于形成嵌合体的两个基因型中保守的区域。B)修饰变体的保守图。

图4-A)被识别为在用于形成嵌合体的两个基因型中保守的区域。B)变体的保守图

图5-被识别为在用于形成嵌合体的两个基因型中保守的区域

图6-被识别为在用于形成嵌合变体的两个基因型中保守的区域

图7-被识别为在用于形成嵌合体的两个基因型中保守的区域

图8-在初免加强疫苗接种后诱导高频率的疫苗特异性T细胞。对来自C57BL/6小鼠(六只/组)的PBMC进行IFNγElispot，所述小鼠用DNA-HPV、MVA-HPV或ChAdOx1-5GHPV3肌肉内初免，并且然后在两周后用异源或同源疫苗肌肉内加强。初免后两周和加强后一周和两周通过尾静脉出血收集PBMC。

图9-初免加强疫苗接种能够诱导对免疫原中编码的所有抗原的应答。在加强两周时收集PBMC并且将其用于肽跨越整个免疫原序列的IFNγElispot中，根据蛋白质来源合并。由于存在大量的肽，将跨越免疫原E2区的肽分成两个库，并且将跨越E4和E5的区域的肽合并到一个库中。

图10-疫苗特异性CD8+T细胞可以在ChAdOx1-5GHPV3初免MVA-5GHPV3加强六周之后在高频下检测到。10a：使用PBMC的IFNγElispot，所述PBMC在加强(DM；DNA-5GHPV3初免MVA-5GHPV3加强，CM；ChAdOx1-5GHPV3初免MVA-5GHPV3加强)后两周、三周、四周和六周后通过尾静脉出血收集。10b：对在ChAdOx1-5GHPV3初免、MVA-5GHPV3加强后一周和六周获得的PBMC进行的ICS。用E6和E7肽库刺激PBMC。测量IFN-γ、CD107、TNF-α和IL2。

图11-HPV E6和E7特异性CD8+T细胞应答是多功能的并且具有细胞毒性潜力。在ChAdOx1-5GHPV3初免MVA-5GHPV3加强后一周通过尾静脉出血收集的PBMC用免疫显性肽库E6和E7以及子库22(其是E6内的显性子库)刺激。应答的CD8+T细胞主要表达三种功能(CD107、IFNγ和TNFα)。

图12–ChAdOx1-5GHPV3初免MVA-5GHPV3加强也引发HPV E6和E7特异性CD4+T细胞应答，这些应答在加强后六周仍可检测到。对在ChAdOx1-5GHPV3初免、MVA-5GHPV3加强后一周和六周获得的PBMC进行的ICS。用E6和E7肽库刺激PBMC。测量IFN-γ、CD107、TNF-α和IL2。

图13-大多数E6和E7特异性CD4+T细胞表达两种功能。在ChAdOx1-5GHPV3初免MVA-5GHPV3加强后一周通过尾静脉出血收集的PBMC用免疫显性肽库E6和E7以及子库22(其是E6内的显性子库)刺激。应答的CD4+T细胞主要表达两种功能(折扣CD107+单功能细胞)。

图14-HPV E6和E7特异性CD8+T细胞可以在子宫颈中检测到。对子宫颈阴道淋巴细胞进行ICS，所述子宫颈阴道淋巴细胞在ChAdOx1-5GHPV3初免MVA-5GHPV3加强并且用免疫显性肽库E6和E7以及子库22刺激后两周从小鼠分离。测量IFN-γ、CD107、TNF-α和IL2。

图15-子宫颈阴道HPV E6和E7特异性CD8+T细胞应答是多功能的。在ChAdOx1-5GHPV3初免MVA-5GHPV3加强后一周收集的子宫颈阴道淋巴细胞用免疫显性肽库E6和E7以及子库22(其是E6内的显性子库)刺激。应答的CD8+T细胞主要表达三种功能(CD107、IFNγ和TNFα)。

图16-子宫颈中疫苗诱导的E6和E7特异性CD8+和CD4+T细胞几乎完全是效应子表型。原始：CD44-CD62L+。抗原处理：中央记忆细胞-CD62L+、CD127+；效应记忆细胞-CD62L-、CD127+；效应细胞-CD62L-、CD127-。由于淋巴细胞数量低，将六只小鼠的子宫颈淋巴细胞合并成三对

图17-与子宫颈相比，脾脏中疫苗诱导的E6和E7特异性CD8+和CD4+T细胞包含效应细胞和效应记忆细胞群。

图18-用ChAdOx1-5GHPV3初免、MVA-5GHPV3加强疫苗接种的小鼠相比于对照小鼠展示存活率提高。在第0天给小鼠接种5x10⁴个TC-1细胞，然后在第3天进行初免，并且在第17天加强。每两天用数字卡尺测量肿瘤并且当肿瘤在任一方向上达到10mm时将小鼠杀死。

图19-远交CD1小鼠的疫苗接种诱导高频T细胞应答。对来自CD1小鼠(十只/组)的PBMC进行IFNγElispot，所述小鼠用DNA-5GHPV3、MVA-5GHPV3或ChAdOx1-5GHPV3肌肉内初免，并且然后在两周后用异源或同源疫苗肌肉内加强。初免后两周和加强后两周和三周通过尾静脉出血收集PBMC。

图20-免疫接种CD1小鼠中的T细胞应答指向整个免疫原。在加强两周时收集PBMC并且将其用于肽跨越整个免疫原序列的IFNγElispot中，根据蛋白质来源合并。由于存在大量的肽，将跨越免疫原E2区的肽分成两个库，并且将跨越E4和E5的区域的肽合并到一个库中。

图21-上图：在离体IFN-γElispot分析中，基于高危HPV(hrHPV)的早期蛋白质，测试来自76位16-24岁女性的外周血单核细胞(PBMC)的肽识别。‘插入物’是由跨越hrHPV转基因的11个氨基酸重叠的15聚体肽库。‘参考’肽是基于来自HPV16和HPV52的早期蛋白质的肽库，所述早期蛋白质组合如下：E1/E2、E4/E5和E6/E7。‘FEC’(流感、EBV和CMV)肽和PHA(植物血凝素)用作阳性对照。所示数据是减去阴性对照值(模拟刺激的细胞)之后从肽刺激的孔中获得的斑点形成单位(SFU)。阳性应答的临界值设定为25SFU/百万个PBMC(从所有供体的模拟刺激值的平均值+2个标准偏差推导)。基于阴道采样同时测试了女性的hrHPV DNA：测试的26％呈阳性。数据显示，具有目前hrHPV感染或先前暴露的9/76名女性识别转基因中编码的HPV序列。下图：通过用插入物库的子库(左侧，每百万个PBMC的SFU)，之后库内的单个肽(右侧，每个孔的SFU)测试PBMC，在一个应答的供体中进一步询问对插入物库的应答，因此证实存在真实的HPV特异性应答。SFU-报告为每孔(含有200000个PBMC)或每百万个PBMC的斑点形成单位。

图22-在ChAdOX1-5G-HPV3初免、MVA-5GHPV3加强后，C57BL/6小鼠(上图)和CD1小鼠(下图)中的子库绘图。在加强后两周将小鼠处死并分离脾细胞。将脾细胞用于IFNγElispot分析中，该分析使用覆盖免疫原序列的子库。子库21、25和26(例如)不含HPV53序列并且仍然获得高幅度应答，因此提供了不含HPV53片段的序列仍将具有免疫原性的证据。

具体实施方式

HPV免疫原设计

HPV免疫原由保守并且在蛋白质水平上地理上代表全局HPV群体的氨基酸片段构成。使用保守性算法创建每个片段，该保守算法已经用于创建基于嵌合或变体的片段。选择取决于所用每种HPV蛋白的特征。

核心保守性算法使用滑动窗口方法与标准化方法的组合，该标准化方法考虑了收集偏差以识别在基因型内部(基因型内)和基因型之间(基因型间)均保守的窗口。基因型内保守的窗口被归类为保守值小于整个序列所有窗口保守值之和的四分之一的窗口。在此过程中，为每个基因型创建标准化的代表性共有序列。随后，基因型间窗口被识别为每个基因型中相同位置的窗口，这些窗口是保守的并且具有>60％共享的共有同一性。

创建代表每种蛋白质的区域的所有选定基因型的序列的一个关键挑战在于许多基因型显示出独特的系统发育聚类。基因型中保守的区域的识别与这些区域之间共享的共有同一性百分比水平之间存在关键的平衡。在许多情况下，基因型间差异过大以致牺牲共享的共有同一性％。一些基因型彼此非常不同，意味着无法创建代表性序列。为了解决这个，原始序列以两种方式之一输入到核心算法中(图1)。(i)变体；将所有基因型的序列输入到算法中并且识别基因型间保守区与共享的共有同一性％无关。从所得区域创建系统发育并且组合内群序列以创建具有高水平共享的共有同一性的共有序列(图1b)；(ii)嵌合体；在一些情况下，基因型之间的距离过于显著以致基因型无法正确比对。因此，仅将基因型内群一起输入到算法中，并且识别具有>60％共享的共有同一性的内群之间的保守区域(图1a)。

HPV候选物

使用从NCBI蛋白质数据库中收集的输入序列为六种HPV蛋白质(E1-2、E4-7)创建候选序列，进行比对并手动审核以除去不完整和质量较差的序列(表1)。

表1|用作输入后审核的序列数。

E1候选物

识别出E1蛋白内的5个片段，这些片段具有在其各自基因型内的相同位置处保守的窗口。支序分类学识别了提供高水平共享的共有同一性的最合适基因型组合(图2)。

E2候选物

由于比对不一致，基于系统发育创建了三个嵌合体(16和31、52和58、53和18)(图3a)。另外，所有基因型都以类似于创建变体的方式输入到算法中，但是没有禁用用于仅从每个基因型中选择具有大于60％共享的共有同一性％的保守窗口的程序过滤器。这识别出一个片段，称为‘修饰变体’(图3b)。

E4候选物

创建了四个嵌合体，其中两个片段是基因型特异性的(图4a)。另外，变体分析识别了一个区域(图4b)。

E5候选物

创建了三个嵌合体(图5)。

E6候选物

E6蛋白在基因型中显示出非常有限的保守性。取代地，处理内群以产生没有共享的共有同一性％过滤器的嵌合体，但是除了基因型52和58，未组合保守窗口以产生共有序列。(图6)

E7候选物

针对基因型16和31以及52和58创建了嵌合体(图7)。基因型53和18是嵌合变体。

具有SEQ ID NO.的疫苗片段的概述

HPV3核苷酸序列(SEQ ID NO:60)

从起始密码子，以用额外接头编码的TPA前导序列开始(TPA和连接子为粗体和下划线)。

不编码肽佐剂/TPA的HPV3核苷酸序列(SEQ ID NO:65)

HPV3多肽序列(SEQ ID NO:61)

从起始密码子，以TPA前导序列开始。

不含TPA/肽佐剂序列的HPV3多肽序列(SEQ ID NO:66)

具有免疫原编码序列插入物的病毒载体序列ChAdOxl.

免疫原编码序列插入物的起始和终止密码子带下划线。前导TPA序列和接头呈粗体。

(SEQ ID NO:62)

ChAdOx2序列(SEQ ID NO:67+68)

免疫原盒的ChAdOx2序列5’以SEQ ID NO:67提供，并且免疫原盒的ChAdOx2序列3’以SEQ ID NO:68提供。

MVA序列(SEQ ID NO:69+70)

免疫原盒的MVA序列5’以SEQ ID NO:69提供，并且免疫原盒的MVA序列3’以SEQ IDNO:70提供。

已经使用两种不同的穿梭质粒制备MVA疫苗：

1.P7.5穿梭质粒。HPV插入物具有与亲本MVA病毒中的TK基因座区域同源的上游和下游侧翼。插入物在p7.5启动子的控制下。

2.F11穿梭质粒。HPV插入物具有与亲本MVA病毒中的F11基因座区域同源的上游和下游侧翼。插入物在F11启动子的控制下。

然后将鸡胚胎成纤维细胞用MVA亲本病毒感染并且用p7.5或F11 MVA穿梭质粒转染，以使其与MVA基因组同源重组。因此获得亲本MVA，其感兴趣基因插入TK基因座(p7.5穿梭质粒)或F11基因座(F11穿梭质粒)处的MVA基因组中。产生两种版本的MVA疫苗。

P7.5穿梭质粒(SEQ ID NO:158)(插入物带下划线)

F11-HPV穿梭质粒(SEQ ID NO:159)(疫苗构建体插入物带下划线)

HPV2随机化核苷酸序列(片段呈随机顺序)(包括tPA前导序列和HindIII克隆接头，带下划线)(SEQ ID NO:71)

HPV2随机化序列多肽(包括tPA前导序列和HindIII克隆接头，带下划线)(SEQ IDNO:72)

HPV53del核苷酸序列(已除去来自HPV53的片段)(包括tPA前导序列和HindIII克隆接头，带下划线)(SEQ ID NO:73)

HPV53del多肽序列(包括tPA前导序列和HindIII克隆接头，带下划线)(SEQ IDNO:74)

HPV3接头核苷酸序列(包括tPA前导序列和HindIII克隆接头，带下划线)(SEQ IDNO:75)

HPV3接头多肽序列(包括tPA前导序列和HindIII克隆接头，带下划线)(SEQ IDNO:76)

序列表

<110> 牛津大学创新有限公司

<120> HPV疫苗

<130> JDM89042P.WOP

<150> GB1713163.2

<151> 2017-08-16

<160> 159

<170> PatentIn 版本3.5

<210> 1

<211> 20

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 1

Asp Glu Asp Glu Thr Ala Tyr Asp Ser Gly Thr Asp Leu Ile Asp Phe

1 5 10 15

Ile Asp Asp Ser

20

<210> 2

<211> 19

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 2

Asp Glu Asn Glu Asn Asp Ser Asp Thr Gly Glu Asp Met Val Asp Phe

1 5 10 15

Ile Asp Asn

<210> 3

<211> 19

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 3

Asp Glu Thr Asp Glu Glu Ser Thr Glu Ser Asp Leu Asp Gly Phe Ile

1 5 10 15

Asp Asn Ser

<210> 4

<211> 15

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 4

Ala Gln Leu Ala Asp Ser Asp Ser Asn Ala Cys Ala Phe Leu Lys

1 5 10 15

<210> 5

<211> 15

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 5

Ala Gln Leu Ala Asp Val Asn Ser Asn Ala Ala Ala Phe Leu Lys

1 5 10 15

<210> 6

<211> 20

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 6

Asn Cys Ile Leu Leu Tyr Gly Ala Ala Asn Thr Gly Lys Ser Leu Phe

1 5 10 15

Gly Met Ser Leu

20

<210> 7

<211> 20

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 7

Asn Cys Leu Val Leu Cys Gly Pro Ala Asn Thr Gly Lys Ser Tyr Phe

1 5 10 15

Gly Met Ser Leu

20

<210> 8

<211> 20

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 8

Asn Cys Leu Val Ile Tyr Gly Pro Pro Asn Thr Gly Lys Ser Cys Phe

1 5 10 15

Ala Met Ser Leu

20

<210> 9

<211> 17

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 9

Trp Pro Tyr Leu His Ser Arg Leu Val Val Phe Thr Phe Pro Asn Pro

1 5 10 15

Phe

<210> 10

<211> 17

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 10

Trp Pro Tyr Leu Glu Ser Arg Ile Thr Val Phe Glu Phe Pro Asn Ala

1 5 10 15

Phe

<210> 11

<211> 17

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 11

Leu Arg Tyr Leu His Ser Arg Ile His Val Leu Gln Phe Leu Asn Pro

1 5 10 15

Phe

<210> 12

<211> 17

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 12

Asn Val Cys Gln Asp Lys Ile Leu Glu His Tyr Glu Asn Asp Ser Lys

1 5 10 15

Asp

<210> 13

<211> 16

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 13

Ile Leu Glu His Tyr Glu Asn Asp Ser Lys Asp Leu Cys Asp His Ile

1 5 10 15

<210> 14

<211> 21

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 14

Cys Asp His Ile Asp Tyr Trp Lys His Ile Arg Leu Glu Cys Ala Ile

1 5 10 15

Met Tyr Lys Ala Arg

20

<210> 15

<211> 17

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 15

Ile Arg Leu Glu Cys Ala Ile Met Tyr Lys Ala Arg Glu Met Gly Phe

1 5 10 15

His

<210> 16

<211> 17

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 16

Ile Arg Leu Glu Cys Ala Ile Met Tyr Lys Ala Arg Glu Met Gly Phe

1 5 10 15

His

<210> 17

<211> 17

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 17

Ile Tyr Ile Cys Glu Asp Ala Gln Cys Thr Val Val Glu Gly Gln Val

1 5 10 15

Asp

<210> 18

<211> 17

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 18

Lys Lys Trp Glu Val His Ala Gly Gly Gln Val Ile Leu Cys Pro Glu

1 5 10 15

Ser

<210> 19

<211> 11

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 19

Gly Gln Arg Arg Ile Lys Arg Pro Arg Ser Glu

1 5 10

<210> 20

<211> 8

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 20

Asn Cys His Pro Asn Lys Leu Leu

1 5

<210> 21

<211> 15

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 21

Ile Leu Lys Cys Leu Arg Tyr Arg Phe Lys Lys His Cys Lys Leu

1 5 10 15

<210> 22

<211> 14

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 22

Ser Ser Thr Trp His Trp Thr Cys His Asp Gly Lys His Lys

1 5 10

<210> 23

<211> 19

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 23

Trp His Trp Thr Cys His Asp Gly Lys His Lys Asn Ala Ile Val Thr

1 5 10 15

Leu Thr Tyr

<210> 24

<211> 34

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 24

Tyr Glu Ala Asp Lys Asn Asp Leu Asn Ala Gln Ile Glu His Trp Lys

1 5 10 15

Leu Ile Arg Met Glu Cys Ala Ile Phe Tyr Lys Ala Lys Glu Leu Gly

20 25 30

Ile Ser

<210> 25

<211> 34

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 25

Ile Cys His Gln Val Val Pro Pro Leu Ala Ala Ser Lys Ala Lys Ala

1 5 10 15

Cys Gln Ala Ile Glu Leu Gln Leu Ala Leu Glu Ala Leu Asn Ala Ser

20 25 30

Pro Tyr

<210> 26

<211> 18

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 26

Asp Glu Trp Thr Leu Gln Gln Thr Ser Leu Glu Met Trp Leu Ala Glu

1 5 10 15

Pro Gln

<210> 27

<211> 29

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 27

Phe Lys Lys His Gly Ile Thr Ile Thr Val Gln Tyr Asp Asn Asp Lys

1 5 10 15

Ala Asn Thr Met Asp Tyr Thr Asn Trp Lys Glu Ile Tyr

20 25

<210> 28

<211> 18

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 28

Val Ile Val Cys Pro Ala Ser Ile Pro Ser Asp Glu Ile Ser Thr Glu

1 5 10 15

Glu Ala

<210> 29

<211> 20

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 29

Asp His Ile Asp Tyr Trp Lys Ala Ile Arg Gln Glu Asn Ala Ile Phe

1 5 10 15

Phe Ala Ala Arg

20

<210> 30

<211> 19

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 30

His Gln Val Val Pro Ala Leu Asn Ile Cys Lys Ala Lys Ala Cys Lys

1 5 10 15

Ala Ile Glu

<210> 31

<211> 21

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 31

Trp Asn Thr Glu Pro Lys His Cys Phe Lys Lys Gly Gly Gln His Ile

1 5 10 15

Glu Val Trp Phe Asp

20

<210> 32

<211> 18

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 32

Tyr Val Ala Trp Asp Ser Val Tyr Tyr Cys Gly Asp Asp Gly Trp Cys

1 5 10 15

Lys Thr

<210> 33

<211> 16

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 33

Glu Ala Glu Lys Tyr Gly Cys Lys Gly Thr Trp Glu Val His Phe Gly

1 5 10 15

<210> 34

<211> 24

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 34

Asn Ser Ile Asp Cys Asn Asp Ser Met Cys Ser Thr Phe Asp Asp Asn

1 5 10 15

Val Ser Ala Thr Glu Leu Val Lys

20

<210> 35

<211> 20

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 35

Asp His Ile Asp Tyr Trp Lys Leu Ile Arg Leu Glu Cys Ala Ile Phe

1 5 10 15

Tyr Lys Ala Arg

20

<210> 36

<211> 11

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 36

Arg Arg Leu Ser Ser Asp Gln Asp Gln Ser Gln

1 5 10

<210> 37

<211> 12

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 37

Leu Val Thr Lys Tyr Pro Leu Leu Lys Leu Leu Ser

1 5 10

<210> 38

<211> 41

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 38

Arg Pro Pro Asn Met Gly Val Lys Ala His Gly Lys Cys Ile Trp Glu

1 5 10 15

Asn Lys Val Phe Ile Val Pro Thr Leu Cys Pro Val Pro Leu Asp Pro

20 25 30

Thr Tyr Pro Leu Leu Lys Leu Leu Thr

35 40

<210> 39

<211> 29

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 39

Thr Gln Thr Thr Thr Pro Glu Asn Thr Ser Leu Val Glu Leu Arg Val

1 5 10 15

Thr Thr Pro Lys Ser Thr Val Val Ile Arg Leu His Leu

20 25

<210> 40

<211> 31

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 40

Thr Thr Arg Tyr Pro Leu Leu Ser Leu Leu Asn Ser Tyr Ser Thr Pro

1 5 10 15

Pro His Arg Ile Pro Ala Pro Cys Pro Trp Ala Pro Gln Arg Pro

20 25 30

<210> 41

<211> 10

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 41

Pro Ile Pro Lys Pro Ser Pro Trp Ala Pro

1 5 10

<210> 42

<211> 10

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 42

Pro Ile Pro Lys Pro Ser Pro Trp Ala Pro

1 5 10

<210> 43

<211> 10

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 43

Pro Arg Pro Pro His Cys Pro Trp Val Pro

1 5 10

<210> 44

<211> 10

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 44

Pro Pro Pro Pro Pro Arg Pro Trp Ala Pro

1 5 10

<210> 45

<211> 26

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 45

Cys Phe Leu Leu Cys Phe Cys Val Leu Leu Cys Val Cys Leu Leu Ile

1 5 10 15

Arg Pro Leu Leu Leu Ser Val Ser Thr Tyr

20 25

<210> 46

<211> 27

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 46

Leu Arg Pro Leu Leu Leu Ser Ile Ser Val Tyr Ala Gln Val Leu Val

1 5 10 15

Leu Val Leu Leu Leu Trp Val Ser Ile Gly Ser

20 25

<210> 47

<211> 28

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 47

Leu Leu Pro Ser Val Cys Met Cys Ala Tyr Ala Trp Val Leu Val Phe

1 5 10 15

Val Tyr Ile Val Val Ile Thr Ser Pro Ala Thr Ala

20 25

<210> 48

<211> 54

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 48

Ile Val Tyr Arg Asp Gly Asn Pro Tyr Ala Val Cys Asp Lys Cys Leu

1 5 10 15

Lys Phe Tyr Ser Lys Ile Ser Glu Tyr Arg His Tyr Cys Tyr Ser Leu

20 25 30

Tyr Gly Thr Thr Leu Glu Gln Gln Tyr Asn Lys Pro Leu Cys Asp Leu

35 40 45

Leu Ile Arg Cys Ile Asn

50

<210> 49

<211> 54

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 49

Val Val Tyr Arg Asp Ser Ile Pro His Ala Ala Cys His Lys Cys Ile

1 5 10 15

Asp Phe Tyr Ser Arg Ile Arg Glu Leu Arg His Tyr Ser Asp Ser Val

20 25 30

Tyr Gly Asp Thr Leu Glu Lys Leu Thr Asn Thr Gly Leu Tyr Asn Leu

35 40 45

Leu Ile Arg Cys Leu Arg

50

<210> 50

<211> 26

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 50

Val Phe Cys Lys Lys Ala Leu Thr Ala Ser Glu Val Tyr Asn Phe Ala

1 5 10 15

Tyr Thr Asp Leu Arg Val Val Tyr Arg Asp

20 25

<210> 51

<211> 18

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 51

Ser Lys Val Arg Lys Leu Arg Tyr Tyr Asn Cys Ser Val Tyr Gly Ala

1 5 10 15

Ser Leu

<210> 52

<211> 26

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 52

Val Tyr Cys Lys Gly Gln Leu Thr Glu Thr Glu Val Leu Asp Phe Ala

1 5 10 15

Phe Thr Asp Leu Thr Ile Val Tyr Arg Asp

20 25

<210> 53

<211> 18

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 53

Ser Lys Val Ser Glu Phe Arg Trp Tyr Arg Tyr Ser Val Tyr Gly Thr

1 5 10 15

Thr Leu

<210> 54

<211> 15

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 54

Cys Val Glu Cys Lys Lys Thr Leu Gln Arg Ser Glu Val Tyr Asp

1 5 10 15

<210> 55

<211> 21

<212> PRT

<213> 人工序列

<220>

<223> CQRPLCPQEKKRHVDLNKRFH

<400> 55

Cys Gln Arg Pro Leu Cys Pro Gln Glu Lys Lys Arg His Val Asp Leu

1 5 10 15

Asn Lys Arg Phe His

20

<210> 56

<211> 21

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 56

Thr Leu His Glu Tyr Met Leu Asp Leu Gln Pro Glu Thr Thr Asp Leu

1 5 10 15

Tyr Cys Tyr Glu Gln

20

<210> 57

<211> 26

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 57

Pro Glu Thr Thr Asp Leu His Cys Tyr Glu Gln Leu Gly Asp Ser Ser

1 5 10 15

Asp Glu Glu Asp Thr Gly Gly Leu Asp Gly

20 25

<210> 58

<211> 39

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 58

Asp Glu Asp Glu Asp Glu Val Asp His Leu Gln Glu Gln Pro Gln Gln

1 5 10 15

Ala Arg Arg Asp Glu Gln His Pro Cys Tyr Leu Ile Glu Thr Gln Cys

20 25 30

Cys Arg Cys Glu Ser Leu Val

35

<210> 59

<211> 37

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 59

Glu Glu Asn Asp Glu Ile Asp Gly Val Asn His Gln His Leu Pro Ala

1 5 10 15

Arg Arg Ala Glu Pro Gln Arg His Thr Met Leu Cys Met Cys Cys Lys

20 25 30

Cys Glu Ala Arg Ile

35

<210> 60

<211> 3960

<212> DNA

<213> 人工序列

<220>

<223> 疫苗构建体

<400> 60

atggatgcta tgaagagggg cctgtgctgc gtgctgctgc tgtgtggcgc cgtgtttgtg 60

tcccccagcc aggaaatcca cgcccggttc agaagaggca gcaagctggc cgacgaggac 120

gagacagcct acgacagcgg caccgacctg atcgacttca tcgacgacag cgacgagaat 180

gagaacgact ccgacaccgg cgaggacatg gtggatttca tcgacaacga cgaaaccgac 240

gaagagagca ccgagagcga cctggacggc tttatcgaca actccgccca gctggctgac 300

agcgacagca atgcctgcgc cttcctgaag gctcagctgg cagacgtgaa cagcaacgcc 360

gctgcttttc tgaagaactg catcctgctg tacggcgctg ccaacaccgg caagagcctg 420

ttcggcatga gcctgaactg cctggtgctg tgcggcccag ccaataccgg aaagtcctac 480

ttcggcatgt ccctgaattg tctcgtgatc tacggcccac ctaacacagg caagtcctgc 540

tttgccatgt ctctgtggcc ctacctgcac agcagactgg tggtgtttac cttccccaac 600

cccttctggc cttacctgga aagccggatc accgtgttcg agttccccaa tgcctttctg 660

agatacctgc actcccggat ccacgtgctg cagtttctga accccttcaa cgtgtgccag 720

gacaagatcc tggaacacta cgagaacgac agcaaggaca ttctggaaca ttatgagaat 780

gattccaagg acctgtgcga ccacatctgc gatcacatcg actactggaa gcacatccgg 840

ctggaatgcg ccatcatgta caaggcccgg atcagactgg aatgtgctat tatgtataag 900

gctcgcgaga tgggcttcca ccagttcgac ggcgacatct gcaacaccat gcactacacc 960

aactggatct atatctgcga ggacgcccag tgcaccgtgg tggaaggcca ggtggacaag 1020

aaatgggagg tgcacgctgg cggccaagtg atcctgtgtc ctgagagcgg ccagcggcgg 1080

atcaagaggc ccagaagcga gaactgccac cccaacaagc tgctgatcct gaagtgcctg 1140

cggtacagat tcaagaagca ctgcaagctg agcagcacct ggcactggac ctgccacgac 1200

ggcaagcaca agtggcattg gacatgtcac gatgggaaac acaagaacgc cattgtgacc 1260

ctgacctact acgaggccga caagaacgac ctgaacgccc agatcgagca ctggaaactg 1320

atccggatgg aatgtgcaat cttctataag gccaaagagc tgggcatcag catctgccac 1380

caggtggtgc ctccactggc cgcctctaaa gccaaagcct gccaggccat cgaactgcag 1440

ctggccctgg aagccctgaa tgccagcccc tacgatgagt ggaccctgca gcagaccagc 1500

ctggaaatgt ggctggccga gccccagttt aagaagcacg gcatcaccat caccgtgcag 1560

tacgacaatg acaaggccaa taccatggat tacacaaatt ggaaagaaat ctacgtgatc 1620

gtgtgccccg ccagcatccc ctccgatgag atcagcaccg aggaagccga ccacattgat 1680

tattggaaag ccatcaggca ggaaaacgcc atcttcttcg ccgccagaca ccaggtggtg 1740

cccgccctga atatctgcaa ggccaaggcc tgtaaagcca tcgagtggaa caccgagccc 1800

aagcactgct tcaagaaggg cggccagcac atcgaagtgt ggttcgacta cgtggcctgg 1860

gacagcgtgt actactgcgg cgacgatggc tggtgcaaga ccgaggccga gaagtacggc 1920

tgcaagggca cctgggaagt gcatttcggc aacagcatcg actgcaacga ctccatgtgc 1980

agcaccttcg acgacaacgt gtccgccacc gagctcgtga aggaccatat cgactattgg 2040

aagctgattc gcctggaatg tgccattttt tacaaggcca gacggcggct gtccagcgac 2100

caggatcagt ctcagctcgt gaccaagtac cccctgctga agctgctgtc cagacccccc 2160

aacatgggcg tgaaggccca cggcaagtgc atctgggaga acaaggtgtt catcgtgccc 2220

accctgtgcc ccgtgcctct ggatccaaca tatcctctgc tgaaactgct gaccacccag 2280

accaccaccc ccgagaatac ctccctggtg gaactgagag tgaccacccc caagagcaca 2340

gtcgtgatca ggctgcacct gaccaccaga tacccactgc tgtcactgct gaacagctac 2400

agcacccccc ctcaccggat ccctgctcca tgtccttggg ctcctcagag gccccccatc 2460

cctaagcctt ctccatgggc ccctagaatc cctgcccctt gcccctgggc acctcctaga 2520

cctccacact gtccatgggt gccccctcca cctcctccaa gaccttgggc cccttgcttc 2580

ctgctgtgct tttgtgtgct gctgtgcgtg tgcctgctga tcagacccct gctgctgagt 2640

gtgtccacct acctgaggcc tctgctgctg tctatcagcg tgtacgctca ggtgctggtg 2700

ctggtgctgc tgctgtgggt gtccatcgga agcctgctgc ccagcgtgtg catgtgtgcc 2760

tatgcctggg tgctggtgtt cgtgtacatc gtcgtgatta ccagccccgc caccgccatc 2820

gtgtaccggg atggcaatcc ttacgccgtg tgcgacaagt gcctgaagtt ctacagcaag 2880

atcagcgagt accggcacta ctgctacagc ctgtacggca ccaccctgga acagcagtac 2940

aacaagcccc tgtgcgatct gctgattcgg tgcatcaacg tggtgtacag agactccatc 3000

ccccacgccg cctgccacaa gtgtatcgac ttctactcca gaatcagaga gctgcggcac 3060

tacagcgact ccgtgtacgg cgataccctg gaaaagctga ccaacactgg cctgtacaac 3120

ctgctgatta gatgcctgcg ggtgttctgc aagaaggccc tgacagccag cgaggtgtac 3180

aacttcgcct acaccgatct gcgggtggtg tatcgggaca gcaaagtgcg gaagctgagg 3240

tactacaact gctctgtgta tggcgccagc ctggtgtatt gcaagggaca gctgaccgag 3300

acagaggtgc tggatttcgc cttcacagac ctgacaatcg tgtatcgcga ctccaaggtg 3360

tccgagttcc ggtggtacag atattccgtg tatggcacca cactgtgcgt ggaatgcaag 3420

aaaaccctgc agagatctga ggtgtacgac tgccagcggc cactgtgtcc gcaggaaaag 3480

aaaagacacg tggacctgaa caagcggttc cacaccctgc acgagtacat gctggatctg 3540

cagcccgaga caaccgacct gtactgctac gagcagcctg aaaccactga tctgcactgt 3600

tatgagcagc tgggagacag ctccgatgaa gaggacactg gcggcctgga tggggacgag 3660

gatgaggacg aagtggacca tctgcaggaa cagccccagc aggctagacg ggacgaacag 3720

cacccttgct atctgatcga gacacagtgc tgcagatgcg aatctctggt ggaagagaac 3780

gacgagatcg acggcgtgaa ccaccagcat ctgcccgcta gaagggccga gcctcagaga 3840

cacaccatgc tgtgtatgtg ctgcaagtgc gaggccagaa tcgccggctc tggacctggc 3900

gcctctggca agcctatccc caatccactg ctgggcctgg actccacccg gacctgataa 3960

<210> 61

<211> 1318

<212> PRT

<213> 人工序列

<220>

<223> 疫苗构建体

<400> 61

Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly

1 5 10 15

Ala Val Phe Val Ser Pro Ser Gln Glu Ile His Ala Arg Phe Arg Arg

20 25 30

Gly Ser Lys Leu Ala Asp Glu Asp Glu Thr Ala Tyr Asp Ser Gly Thr

35 40 45

Asp Leu Ile Asp Phe Ile Asp Asp Ser Asp Glu Asn Glu Asn Asp Ser

50 55 60

Asp Thr Gly Glu Asp Met Val Asp Phe Ile Asp Asn Asp Glu Thr Asp

65 70 75 80

Glu Glu Ser Thr Glu Ser Asp Leu Asp Gly Phe Ile Asp Asn Ser Ala

85 90 95

Gln Leu Ala Asp Ser Asp Ser Asn Ala Cys Ala Phe Leu Lys Ala Gln

100 105 110

Leu Ala Asp Val Asn Ser Asn Ala Ala Ala Phe Leu Lys Asn Cys Ile

115 120 125

Leu Leu Tyr Gly Ala Ala Asn Thr Gly Lys Ser Leu Phe Gly Met Ser

130 135 140

Leu Asn Cys Leu Val Leu Cys Gly Pro Ala Asn Thr Gly Lys Ser Tyr

145 150 155 160

Phe Gly Met Ser Leu Asn Cys Leu Val Ile Tyr Gly Pro Pro Asn Thr

165 170 175

Gly Lys Ser Cys Phe Ala Met Ser Leu Trp Pro Tyr Leu His Ser Arg

180 185 190

Leu Val Val Phe Thr Phe Pro Asn Pro Phe Trp Pro Tyr Leu Glu Ser

195 200 205

Arg Ile Thr Val Phe Glu Phe Pro Asn Ala Phe Leu Arg Tyr Leu His

210 215 220

Ser Arg Ile His Val Leu Gln Phe Leu Asn Pro Phe Asn Val Cys Gln

225 230 235 240

Asp Lys Ile Leu Glu His Tyr Glu Asn Asp Ser Lys Asp Ile Leu Glu

245 250 255

His Tyr Glu Asn Asp Ser Lys Asp Leu Cys Asp His Ile Cys Asp His

260 265 270

Ile Asp Tyr Trp Lys His Ile Arg Leu Glu Cys Ala Ile Met Tyr Lys

275 280 285

Ala Arg Ile Arg Leu Glu Cys Ala Ile Met Tyr Lys Ala Arg Glu Met

290 295 300

Gly Phe His Gln Phe Asp Gly Asp Ile Cys Asn Thr Met His Tyr Thr

305 310 315 320

Asn Trp Ile Tyr Ile Cys Glu Asp Ala Gln Cys Thr Val Val Glu Gly

325 330 335

Gln Val Asp Lys Lys Trp Glu Val His Ala Gly Gly Gln Val Ile Leu

340 345 350

Cys Pro Glu Ser Gly Gln Arg Arg Ile Lys Arg Pro Arg Ser Glu Asn

355 360 365

Cys His Pro Asn Lys Leu Leu Ile Leu Lys Cys Leu Arg Tyr Arg Phe

370 375 380

Lys Lys His Cys Lys Leu Ser Ser Thr Trp His Trp Thr Cys His Asp

385 390 395 400

Gly Lys His Lys Trp His Trp Thr Cys His Asp Gly Lys His Lys Asn

405 410 415

Ala Ile Val Thr Leu Thr Tyr Tyr Glu Ala Asp Lys Asn Asp Leu Asn

420 425 430

Ala Gln Ile Glu His Trp Lys Leu Ile Arg Met Glu Cys Ala Ile Phe

435 440 445

Tyr Lys Ala Lys Glu Leu Gly Ile Ser Ile Cys His Gln Val Val Pro

450 455 460

Pro Leu Ala Ala Ser Lys Ala Lys Ala Cys Gln Ala Ile Glu Leu Gln

465 470 475 480

Leu Ala Leu Glu Ala Leu Asn Ala Ser Pro Tyr Asp Glu Trp Thr Leu

485 490 495

Gln Gln Thr Ser Leu Glu Met Trp Leu Ala Glu Pro Gln Phe Lys Lys

500 505 510

His Gly Ile Thr Ile Thr Val Gln Tyr Asp Asn Asp Lys Ala Asn Thr

515 520 525

Met Asp Tyr Thr Asn Trp Lys Glu Ile Tyr Val Ile Val Cys Pro Ala

530 535 540

Ser Ile Pro Ser Asp Glu Ile Ser Thr Glu Glu Ala Asp His Ile Asp

545 550 555 560

Tyr Trp Lys Ala Ile Arg Gln Glu Asn Ala Ile Phe Phe Ala Ala Arg

565 570 575

His Gln Val Val Pro Ala Leu Asn Ile Cys Lys Ala Lys Ala Cys Lys

580 585 590

Ala Ile Glu Trp Asn Thr Glu Pro Lys His Cys Phe Lys Lys Gly Gly

595 600 605

Gln His Ile Glu Val Trp Phe Asp Tyr Val Ala Trp Asp Ser Val Tyr

610 615 620

Tyr Cys Gly Asp Asp Gly Trp Cys Lys Thr Glu Ala Glu Lys Tyr Gly

625 630 635 640

Cys Lys Gly Thr Trp Glu Val His Phe Gly Asn Ser Ile Asp Cys Asn

645 650 655

Asp Ser Met Cys Ser Thr Phe Asp Asp Asn Val Ser Ala Thr Glu Leu

660 665 670

Val Lys Asp His Ile Asp Tyr Trp Lys Leu Ile Arg Leu Glu Cys Ala

675 680 685

Ile Phe Tyr Lys Ala Arg Arg Arg Leu Ser Ser Asp Gln Asp Gln Ser

690 695 700

Gln Leu Val Thr Lys Tyr Pro Leu Leu Lys Leu Leu Ser Arg Pro Pro

705 710 715 720

Asn Met Gly Val Lys Ala His Gly Lys Cys Ile Trp Glu Asn Lys Val

725 730 735

Phe Ile Val Pro Thr Leu Cys Pro Val Pro Leu Asp Pro Thr Tyr Pro

740 745 750

Leu Leu Lys Leu Leu Thr Thr Gln Thr Thr Thr Pro Glu Asn Thr Ser

755 760 765

Leu Val Glu Leu Arg Val Thr Thr Pro Lys Ser Thr Val Val Ile Arg

770 775 780

Leu His Leu Thr Thr Arg Tyr Pro Leu Leu Ser Leu Leu Asn Ser Tyr

785 790 795 800

Ser Thr Pro Pro His Arg Ile Pro Ala Pro Cys Pro Trp Ala Pro Gln

805 810 815

Arg Pro Pro Ile Pro Lys Pro Ser Pro Trp Ala Pro Arg Ile Pro Ala

820 825 830

Pro Cys Pro Trp Ala Pro Pro Arg Pro Pro His Cys Pro Trp Val Pro

835 840 845

Pro Pro Pro Pro Pro Arg Pro Trp Ala Pro Cys Phe Leu Leu Cys Phe

850 855 860

Cys Val Leu Leu Cys Val Cys Leu Leu Ile Arg Pro Leu Leu Leu Ser

865 870 875 880

Val Ser Thr Tyr Leu Arg Pro Leu Leu Leu Ser Ile Ser Val Tyr Ala

885 890 895

Gln Val Leu Val Leu Val Leu Leu Leu Trp Val Ser Ile Gly Ser Leu

900 905 910

Leu Pro Ser Val Cys Met Cys Ala Tyr Ala Trp Val Leu Val Phe Val

915 920 925

Tyr Ile Val Val Ile Thr Ser Pro Ala Thr Ala Ile Val Tyr Arg Asp

930 935 940

Gly Asn Pro Tyr Ala Val Cys Asp Lys Cys Leu Lys Phe Tyr Ser Lys

945 950 955 960

Ile Ser Glu Tyr Arg His Tyr Cys Tyr Ser Leu Tyr Gly Thr Thr Leu

965 970 975

Glu Gln Gln Tyr Asn Lys Pro Leu Cys Asp Leu Leu Ile Arg Cys Ile

980 985 990

Asn Val Val Tyr Arg Asp Ser Ile Pro His Ala Ala Cys His Lys Cys

995 1000 1005

Ile Asp Phe Tyr Ser Arg Ile Arg Glu Leu Arg His Tyr Ser Asp

1010 1015 1020

Ser Val Tyr Gly Asp Thr Leu Glu Lys Leu Thr Asn Thr Gly Leu

1025 1030 1035

Tyr Asn Leu Leu Ile Arg Cys Leu Arg Val Phe Cys Lys Lys Ala

1040 1045 1050

Leu Thr Ala Ser Glu Val Tyr Asn Phe Ala Tyr Thr Asp Leu Arg

1055 1060 1065

Val Val Tyr Arg Asp Ser Lys Val Arg Lys Leu Arg Tyr Tyr Asn

1070 1075 1080

Cys Ser Val Tyr Gly Ala Ser Leu Val Tyr Cys Lys Gly Gln Leu

1085 1090 1095

Thr Glu Thr Glu Val Leu Asp Phe Ala Phe Thr Asp Leu Thr Ile

1100 1105 1110

Val Tyr Arg Asp Ser Lys Val Ser Glu Phe Arg Trp Tyr Arg Tyr

1115 1120 1125

Ser Val Tyr Gly Thr Thr Leu Cys Val Glu Cys Lys Lys Thr Leu

1130 1135 1140

Gln Arg Ser Glu Val Tyr Asp Cys Gln Arg Pro Leu Cys Pro Gln

1145 1150 1155

Glu Lys Lys Arg His Val Asp Leu Asn Lys Arg Phe His Thr Leu

1160 1165 1170

His Glu Tyr Met Leu Asp Leu Gln Pro Glu Thr Thr Asp Leu Tyr

1175 1180 1185

Cys Tyr Glu Gln Pro Glu Thr Thr Asp Leu His Cys Tyr Glu Gln

1190 1195 1200

Leu Gly Asp Ser Ser Asp Glu Glu Asp Thr Gly Gly Leu Asp Gly

1205 1210 1215

Asp Glu Asp Glu Asp Glu Val Asp His Leu Gln Glu Gln Pro Gln

1220 1225 1230

Gln Ala Arg Arg Asp Glu Gln His Pro Cys Tyr Leu Ile Glu Thr

1235 1240 1245

Gln Cys Cys Arg Cys Glu Ser Leu Val Glu Glu Asn Asp Glu Ile

1250 1255 1260

Asp Gly Val Asn His Gln His Leu Pro Ala Arg Arg Ala Glu Pro

1265 1270 1275

Gln Arg His Thr Met Leu Cys Met Cys Cys Lys Cys Glu Ala Arg

1280 1285 1290

Ile Ala Gly Ser Gly Pro Gly Ala Ser Gly Lys Pro Ile Pro Asn

1295 1300 1305

Pro Leu Leu Gly Leu Asp Ser Thr Arg Thr

1310 1315

<210> 62

<211> 42783

<212> DNA

<213> 人工序列

<220>

<223> 病毒载体构建体

<400> 62

gtttaaacgc ggccgccagg cctacccact agtcaattcg ggaggatcga aacggcagat 60

cgcaaaaaac agtacataca gaaggagaca tgaacatgaa catcaaaaaa attgtaaaac 120

aagccacagt tctgactttt acgactgcac ttctggcagg aggagcgact caagccttcg 180

cgaaagaaaa taaccaaaaa gcatacaaag aaacgtacgg cgtctctcat attacacgcc 240

atgatatgct gcagatccct aaacagcagc aaaacgaaaa ataccaagtg cctcaattcg 300

atcaatcaac gattaaaaat attgagtctg caaaaggact tgatgtgtgg gacagctggc 360

cgctgcaaaa cgctgacgga acagtagctg aatacaacgg ctatcacgtt gtgtttgctc 420

ttgcgggaag cccgaaagac gctgatgaca catcaatcta catgttttat caaaaggtcg 480

gcgacaactc aatcgacagc tggaaaaacg cgggccgtgt ctttaaagac agcgataagt 540

tcgacgccaa cgatccgatc ctgaaagatc agacgcaaga atggtccggt tctgcaacct 600

ttacatctga cggaaaaatc cgtttattct acactgacta ttccggtaaa cattacggca 660

aacaaagcct gacaacagcg caggtaaatg tgtcaaaatc tgatgacaca ctcaaaatca 720

acggagtgga agatcacaaa acgatttttg acggagacgg aaaaacatat cagaacgttc 780

agcagtttat cgatgaaggc aattatacat ccggcgacaa ccatacgctg agagaccctc 840

actacgttga agacaaaggc cataaatacc ttgtattcga agccaacacg ggaacagaaa 900

acggatacca aggcgaagaa tctttattta acaaagcgta ctacggcggc ggcacgaact 960

tcttccgtaa agaaagccag aagcttcagc agagcgctaa aaaacgcgat gctgagttag 1020

cgaacggcgc cctcggtatc atagagttaa ataatgatta cacattgaaa aaagtaatga 1080

agccgctgat cacttcaaac acggtaactg atgaaatcga gcgcgcgaat gttttcaaaa 1140

tgaacggcaa atggtacttg ttcactgatt cacgcggttc aaaaatgacg atcgatggta 1200

ttaactcaaa cgatatttac atgcttggtt atgtatcaaa ctctttaacc ggcccttaca 1260

agccgctgaa caaaacaggg cttgtgctgc aaatgggtct tgatccaaac gatgtgacat 1320

tcacttactc tcacttcgca gtgccgcaag ccaaaggcaa caatgtggtt atcacaagct 1380

acatgacaaa cagaggcttc ttcgaggata aaaaggcaac atttgcgcca agcttcttaa 1440

tgaacatcaa aggcaataaa acatccgttg tcaaaaacag catcctggag caaggacagc 1500

tgacagtcaa ctaataacag caaaaagaaa atgccgatac ttcattggca ttttctttta 1560

tttctcaaca agatggtgaa ttgactagtg ggtagatcca caggacgggt gtggtcgcca 1620

tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa 1680

agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg 1740

ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc 1800

ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga 1860

tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact 1920

gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattgatcc 1980

ggaaccctta atataacttc gtataatgta tgctatacga agttattagg tccctcgact 2040

atagggtcac cgtcgacagc gacacacttg catcggatgc agcccggtta acgtgccggc 2100

acggcctggg taaccaggta ttttgtccac ataaccgtgc gcaaaatgtt gtggataagc 2160

aggacacagc agcaatccac agcaggcata caaccgcaca ccgaggttac tccgttctac 2220

aggttacgac gacatgtcaa tacttgccct tgacaggcat tgatggaatc gtagtctcac 2280

gctgatagtc tgatcgacaa tacaagtggg accgtggtcc cagaccgata atcagaccga 2340

crayacgagt gggaycgtgg tcccagacta ataatcagac cgacgatacg agtgggaccg 2400

tggtcccaga ctaataatca gaccgacgat acgagtggga ccgtggtycc agwctratwa 2460

tcagaccgac gatacragtg gracmgtggk cccagasaka atawtcagrc cgagwtaygc 2520

wktckggcct gtaacaaagg acattaagta aagacagata mrmgtgrgac taaaacgtgg 2580

tcccagtctg attatcagac cgacgatacg agtgggaccg tggtcccaga ctaataatca 2640

gaccgacgat acgagtggga ccgtggtccc agactaataa tcagaccgac gatacgagtg 2700

ggaccgtggt cccagtctga ttatcagacc gacgatacaa gtggaacagt gggcccagag 2760

agaatattca ggccagttat gctttctggc ctgtaacaaa ggacattaag taaagacaga 2820

taaacgtaga ctaaaacgtg gtcgcatcag ggtgctggct tttcaagttc cttaagaatg 2880

gcctcaattt tctctataca ctcagttgga acacgagacc tgtccaggtt aagcaccatt 2940

ttatcgccct tatacaatac tgtcgctcca ggagcaaact gatgtcgtga gcttaaacta 3000

gttcttgatg cagatgacgt tttaagcaca gaagttaaaa gagtgataac ttcttcagct 3060

tcaaatatca ccccagcttt tttctgctca tgaaggttag atgcctgctg cttaagtaat 3120

tcctctttat ctgtaaaggc tttttgaagt gcatcacctg accgggcaga tagttcaccg 3180

gggtgagaaa aaagagcaac aactgattta ggcaatttgg cggtgttgat acagcgggta 3240

ataatcttac gtgaaatatt ttccgcatca gccagcgcag aaatatttcc agcaaattca 3300

ttctgcaatc ggcttgcata acgctgacca cgttcataag cacttgttgg gcgataatcg 3360

ttacccaatc tggataatgc agccatctgc tcatcatcca gctcgccaac cagaacacga 3420

taatcacttt cggtaagtgc agcagcttta cgacggcgac tcccatcggc aatttctatg 3480

acaccagata ctcttcgacc gaacgccggt gtctgttgac cagtcagtag aaaagaaggg 3540

atgagatcat ccagtgcgtc ctcagtaagc agctcctggt cacgttcatt acctgaccat 3600

acccgagagg tcttctcaac actatcaccc cggagcactt caagagtaaa cttcacatcc 3660

cgaccacata caggcaaagt aatggcatta ccgcgagcca ttactcctac gcgcgcaatt 3720

aacgaatcca ccatcggggc agctggtgtc gataacgaag tatcttcaac cggttgagta 3780

ttgagcgtat gttttggaat aacaggcgca cgcttcatta tctaatctcc cagcgtggtt 3840

taatcagacg atcgaaaatt tcattgcaga caggttccca aatagaaaga gcatttctcc 3900

aggcaccagt tgaagagcgt tgatcaatgg cctgttcaaa aacagttctc atccggatct 3960

gacctttacc aacttcatcc gtttcacgta caacattttt tagaaccatg cttccccagg 4020

catcccgaat ttgctcctcc atccacgggg actgagagcc attactattg ctgtatttgg 4080

taagcaaaat acgtacatca ggctcgaacc ctttaagatc aacgttcttg agcagatcac 4140

gaagcatatc gaaaaactgc agtgcggagg tgtagtcaaa caactcagca ggcgtgggaa 4200

caatcagcac atcagcagca catacgacat taatcgtgcc gatacccagg ttaggcgcgc 4260

tgtcaataac tatgacatca tagtcatgag caacagtttc aatggccagt cggagcatca 4320

ggtgtggatc ggtgggcagt ttaccttcat caaatttgcc cattaactca gtttcaatac 4380

ggtgcagagc cagacaggaa ggaataatgt caagccccgg ccagcaagtg ggctttattg 4440

cataagtgac atcgtccttt tccccaagat agaaaggcag gagagtgtct tctgcatgaa 4500

tatgaagatc tggtacccat ccgtgataca ttgaggctgt tccctggggg tcgttacctt 4560

ccacgagcaa aacacgtagc cccttcagag ccagatcctg agcaagatga acagaaactg 4620

aggttttgta aacgccacct ttatgggcag caaccccgat caccggtgga aatacgtctt 4680

cagcacgtcg caatcgcgta ccaaacacat cacgcatatg attaatttgt tcaattgtat 4740

aaccaacacg ttgctcaacc cgtcctcgaa tttccatatc cgggtgcggt agtcgccctg 4800

ctttctcggc atctctgata gcctgagaag aaaccccaac taaatccgct gcttcaccta 4860

ttctccagcg ccgggttatt ttcctcgctt ccgggctgtc atcattaaac tgtgcaatgg 4920

cgatagcctt cgtcatttca tgaccagcgt ttatgcactg gttaagtgtt tccatgagtt 4980

tcattctgaa catcctttaa tcattgcttt gcgttttttt attaaatctt gcaatttact 5040

gcaaagcaac aacaaaatcg caaagtcatc aaaaaaccgc aaagttgttt aaaataagag 5100

caacactaca aaaggagata agaagagcac atacctcagt cacttattat cactagcgct 5160

cgccgcagcc gtgtaaccga gcatagcgag cgaactggcg aggaagcaaa gaagaactgt 5220

tctgtcagat agctcttacg ctcagcgcaa gaagaaatat ccaccgtggg aaaaactcca 5280

ggtagaggta cacacgcgga tagccaattc agagtaataa actgtgataa tcaaccctca 5340

tcaatgatga cgaactaacc cccgatatca ggtcacatga cgaagggaaa gagaaggaaa 5400

tcaactgtga caaactgccc tcaaatttgg cttccttaaa aattacagtt caaaaagtat 5460

gagaaaatcc atgcaggctg aaggaaacag caaaactgtg acaaattacc ctcagtaggt 5520

cagaacaaat gtgacgaacc accctcaaat ctgtgacaga taaccctcag actatcctgt 5580

cgtcatggaa gtgatatcgc ggaaggaaaa tacgatatga gtcgtctggc ggcctttctt 5640

tttctcaatg tatgagaggc gcattggagt tctgctgttg atctcattaa cacagacctg 5700

caggaagcgg cggcggaagt caggcatacg ctggtaactt tgaggcagct ggtaacgctc 5760

tatgatccag tcgattttca gagagacgat gcctgagcca tccggcttac gatactgaca 5820

cagggattcg tataaacgca tggcatacgg attggtgatt tcttttgttt cactaagccg 5880

aaactgcgta aaccggttct gtaacccgat aaagaaggga atgagatatg ggttgatatg 5940

tacactgtaa agccctctgg atggactgtg cgcacgtttg ataaaccaag gaaaagattc 6000

atagcctttt tcatcgccgg catcctcttc agggcgataa aaaaccactt ccttccccgc 6060

gaaactcttc aatgcctgcc gtatatcctt actggcttcc gcagaggtca atccgaatat 6120

ttcagcatat ttagcaacat ggatctcgca gataccgtca tgttcctgta gggtgccatc 6180

agattttctg atctggtcaa cgaacagata cagcatacgt ttttgatccc gggagagact 6240

atatgccgcc tcagtgaggt cgtttgactg gacgattcgc gggctatttt tacgtttctt 6300

gtgattgata accgctgttt ccgccatgac agatccatgt gaagtgtgac aagtttttag 6360

attgtcacac taaataaaaa agagtcaata agcagggata actttgtgaa aaaacagctt 6420

cttctgaggg caatttgtca cagggttaag ggcaatttgt cacagacagg actgtcattt 6480

gagggtgatt tgtcacactg aaagggcaat ttgtcacaac accttctcta gaaccagcat 6540

ggataaaggc ctacaaggcg ctctaaaaaa gaagatctaa aaactataaa aaaaataatt 6600

ataaaaatat ccccgtggat aagtggataa ccccaaggga agttttttca ggcatcgtgt 6660

gtaagcagaa tatataagtg ctgttccctg gtgcttcctc gctcactcga gggcttcgcc 6720

ctgtcgctca actgcggcga gcactactgg ctgtaaaagg acagaccaca tcatggttct 6780

gtgttcatta ggttgttctg tccattgctg acataatccg ctccacttca acgtaacacc 6840

gcacgaagat ttctattgtt cctgaaggca tattcaaatc gttttcgtta ccgcttgcag 6900

gcatcatgac agaacactac ttcctataaa cgctacacag gctcctgaga ttaataatgc 6960

ggatctctac gataatggga gattttcccg actgtttcgt tcgcttctca gtggataaca 7020

gccagcttct ctgtttaaca gacaaaaaca gcatatccac tcagttccac atttccatat 7080

aaaggccaag gcatttattc tcaggataat tgtttcagca tcgcaaccgc atcagactcc 7140

ggcatcgcaa actgcacccg gtgccgggca gccacatcca gcgcaaaaac cttcgtgtag 7200

acttccgttg aactgatgga cttatgtccc atcaggcttt gcagaacttt cagcggtata 7260

ccggcataca gcatgtgcat cgcataggaa tggcggaacg tatgtggtgt gaccggaaca 7320

gagaacgtca caccgtcagc agcagcggcg gcaaccgcct ccccaatcca ggtcctgacc 7380

gttctgtccg tcacttccca gatccgcgct ttctctgtcc ttcctgtgcg acggttacgc 7440

cgctccatga gcttatcgcg aataaatacc tgtgacggaa gatcacttcg cagaataaat 7500

aaatcctggt gtccctgttg ataccgggaa gccctgggcc aacttttggc gaaaatgaga 7560

cgttgatcgg cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg 7620

cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat 7680

cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt 7740

tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt 7800

aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg 7860

cctgatgaat gctcatccgg agttccgtat ggcaatgaaa gacggtgagc tggtgatatg 7920

ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct 7980

ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc 8040

gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt 8100

ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa 8160

cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat 8220

gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct 8280

taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag 8340

ttattggtgc ccttaaacgc ctggttgcta cgcctgaata agtgataata agcggatgaa 8400

tggcagaaat tcgatgataa gctgtcaaac atgagaattg gtcgacggcg cgccaaagct 8460

tgcatgcctg cagccgcgta acctggcaaa atcggttacg gttgagtaat aaatggatgc 8520

cctgcgtaag cggggcacat ttcattacct ctttctccgc acccgacata gataataact 8580

tcgtatagta tacattatac gaagttatct agtagactta atcgcgttta aacccatcat 8640

caataatata cctcaaactt tttgtgcgcg ttaatatgca aatgaggcgt ttgaatttgg 8700

gaagggagga aggtgattgg ccgagagaag ggcgaccgtt aggggcgggg cgagtgacgt 8760

tttgatgacg tgaccgcgag gaggagccag tttgcaagtt ctcgtgggaa aagtgacgtc 8820

aaacgaggtg tggtttgaac acggaaatac tcaattttcc cgcgctctct gacaggaaat 8880

gaggtgtttc taggcggatg caagtgaaaa cgggccattt tcgcgcgaaa actgaatgag 8940

gaagtgaaaa tctgagtaat ttcgcgttta tgacagggag gagtatttgc cgagggccga 9000

gtagactttg accgattacg tgggggtttc gattaccgtg tttttcacct aaatttccgc 9060

gtacggtgtc aaagtccggt gtttttacgt aggtgtcagc tgatcgccag ggtatttaaa 9120

cctgcgctct ccagtcaaga ggccactctt gagtgccagc gagaagagtt ttctcctccg 9180

cgcgcgagtc agatctacac tttgaaaggc gatcgctagc gacatcgatc acaagtttgt 9240

acaaaaaagc aggctccacc atgggaacca attcagtcga gcctttcact cattagatgc 9300

atgtcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 9360

cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 9420

gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 9480

tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc 9540

agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta 9600

ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac 9660

ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc 9720

aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc 9780

gtgtacggtg ggaggtctat ataagcagag ctctccctat cagtgataga gatctcccta 9840

tcagtgatag agatcgtcga cgagctcgtt tagtgaaccg tcagatcgcc tggagacgcc 9900

atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc cggttaagct 9960

cggtaccgct agccgcgccg ccaccatgga tgctatgaag aggggcctgt gctgcgtgct 10020

gctgctgtgt ggcgccgtgt ttgtgtcccc cagccaggaa atccacgccc ggttcagaag 10080

aggcagcaag ctggccgacg aggacgagac agcctacgac agcggcaccg acctgatcga 10140

cttcatcgac gacagcgacg agaatgagaa cgactccgac accggcgagg acatggtgga 10200

tttcatcgac aacgacgaaa ccgacgaaga gagcaccgag agcgacctgg acggctttat 10260

cgacaactcc gcccagctgg ctgacagcga cagcaatgcc tgcgccttcc tgaaggctca 10320

gctggcagac gtgaacagca acgccgctgc ttttctgaag aactgcatcc tgctgtacgg 10380

cgctgccaac accggcaaga gcctgttcgg catgagcctg aactgcctgg tgctgtgcgg 10440

cccagccaat accggaaagt cctacttcgg catgtccctg aattgtctcg tgatctacgg 10500

cccacctaac acaggcaagt cctgctttgc catgtctctg tggccctacc tgcacagcag 10560

actggtggtg tttaccttcc ccaacccctt ctggccttac ctggaaagcc ggatcaccgt 10620

gttcgagttc cccaatgcct ttctgagata cctgcactcc cggatccacg tgctgcagtt 10680

tctgaacccc ttcaacgtgt gccaggacaa gatcctggaa cactacgaga acgacagcaa 10740

ggacattctg gaacattatg agaatgattc caaggacctg tgcgaccaca tctgcgatca 10800

catcgactac tggaagcaca tccggctgga atgcgccatc atgtacaagg cccggatcag 10860

actggaatgt gctattatgt ataaggctcg cgagatgggc ttccaccagt tcgacggcga 10920

catctgcaac accatgcact acaccaactg gatctatatc tgcgaggacg cccagtgcac 10980

cgtggtggaa ggccaggtgg acaagaaatg ggaggtgcac gctggcggcc aagtgatcct 11040

gtgtcctgag agcggccagc ggcggatcaa gaggcccaga agcgagaact gccaccccaa 11100

caagctgctg atcctgaagt gcctgcggta cagattcaag aagcactgca agctgagcag 11160

cacctggcac tggacctgcc acgacggcaa gcacaagtgg cattggacat gtcacgatgg 11220

gaaacacaag aacgccattg tgaccctgac ctactacgag gccgacaaga acgacctgaa 11280

cgcccagatc gagcactgga aactgatccg gatggaatgt gcaatcttct ataaggccaa 11340

agagctgggc atcagcatct gccaccaggt ggtgcctcca ctggccgcct ctaaagccaa 11400

agcctgccag gccatcgaac tgcagctggc cctggaagcc ctgaatgcca gcccctacga 11460

tgagtggacc ctgcagcaga ccagcctgga aatgtggctg gccgagcccc agtttaagaa 11520

gcacggcatc accatcaccg tgcagtacga caatgacaag gccaatacca tggattacac 11580

aaattggaaa gaaatctacg tgatcgtgtg ccccgccagc atcccctccg atgagatcag 11640

caccgaggaa gccgaccaca ttgattattg gaaagccatc aggcaggaaa acgccatctt 11700

cttcgccgcc agacaccagg tggtgcccgc cctgaatatc tgcaaggcca aggcctgtaa 11760

agccatcgag tggaacaccg agcccaagca ctgcttcaag aagggcggcc agcacatcga 11820

agtgtggttc gactacgtgg cctgggacag cgtgtactac tgcggcgacg atggctggtg 11880

caagaccgag gccgagaagt acggctgcaa gggcacctgg gaagtgcatt tcggcaacag 11940

catcgactgc aacgactcca tgtgcagcac cttcgacgac aacgtgtccg ccaccgagct 12000

cgtgaaggac catatcgact attggaagct gattcgcctg gaatgtgcca ttttttacaa 12060

ggccagacgg cggctgtcca gcgaccagga tcagtctcag ctcgtgacca agtaccccct 12120

gctgaagctg ctgtccagac cccccaacat gggcgtgaag gcccacggca agtgcatctg 12180

ggagaacaag gtgttcatcg tgcccaccct gtgccccgtg cctctggatc caacatatcc 12240

tctgctgaaa ctgctgacca cccagaccac cacccccgag aatacctccc tggtggaact 12300

gagagtgacc acccccaaga gcacagtcgt gatcaggctg cacctgacca ccagataccc 12360

actgctgtca ctgctgaaca gctacagcac cccccctcac cggatccctg ctccatgtcc 12420

ttgggctcct cagaggcccc ccatccctaa gccttctcca tgggccccta gaatccctgc 12480

cccttgcccc tgggcacctc ctagacctcc acactgtcca tgggtgcccc ctccacctcc 12540

tccaagacct tgggcccctt gcttcctgct gtgcttttgt gtgctgctgt gcgtgtgcct 12600

gctgatcaga cccctgctgc tgagtgtgtc cacctacctg aggcctctgc tgctgtctat 12660

cagcgtgtac gctcaggtgc tggtgctggt gctgctgctg tgggtgtcca tcggaagcct 12720

gctgcccagc gtgtgcatgt gtgcctatgc ctgggtgctg gtgttcgtgt acatcgtcgt 12780

gattaccagc cccgccaccg ccatcgtgta ccgggatggc aatccttacg ccgtgtgcga 12840

caagtgcctg aagttctaca gcaagatcag cgagtaccgg cactactgct acagcctgta 12900

cggcaccacc ctggaacagc agtacaacaa gcccctgtgc gatctgctga ttcggtgcat 12960

caacgtggtg tacagagact ccatccccca cgccgcctgc cacaagtgta tcgacttcta 13020

ctccagaatc agagagctgc ggcactacag cgactccgtg tacggcgata ccctggaaaa 13080

gctgaccaac actggcctgt acaacctgct gattagatgc ctgcgggtgt tctgcaagaa 13140

ggccctgaca gccagcgagg tgtacaactt cgcctacacc gatctgcggg tggtgtatcg 13200

ggacagcaaa gtgcggaagc tgaggtacta caactgctct gtgtatggcg ccagcctggt 13260

gtattgcaag ggacagctga ccgagacaga ggtgctggat ttcgccttca cagacctgac 13320

aatcgtgtat cgcgactcca aggtgtccga gttccggtgg tacagatatt ccgtgtatgg 13380

caccacactg tgcgtggaat gcaagaaaac cctgcagaga tctgaggtgt acgactgcca 13440

gcggccactg tgtccgcagg aaaagaaaag acacgtggac ctgaacaagc ggttccacac 13500

cctgcacgag tacatgctgg atctgcagcc cgagacaacc gacctgtact gctacgagca 13560

gcctgaaacc actgatctgc actgttatga gcagctggga gacagctccg atgaagagga 13620

cactggcggc ctggatgggg acgaggatga ggacgaagtg gaccatctgc aggaacagcc 13680

ccagcaggct agacgggacg aacagcaccc ttgctatctg atcgagacac agtgctgcag 13740

atgcgaatct ctggtggaag agaacgacga gatcgacggc gtgaaccacc agcatctgcc 13800

cgctagaagg gccgagcctc agagacacac catgctgtgt atgtgctgca agtgcgaggc 13860

cagaatcgcc ggctctggac ctggcgcctc tggcaagcct atccccaatc cactgctggg 13920

cctggactcc acccggacct gataagcggc cgctcgagca tgcatctaga gggccctatt 13980

ctatagtgtc acctaaatgc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc 14040

cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc 14100

actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct 14160

attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg 14220

catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg 14280

aggggggatc gatcccgtcg agatatctag acccagcttt cttgtacaaa gtggtgatcg 14340

attcgacaga tcgcgatcgc agtgagtagt gttctggggc gggggaggac ctgcatgagg 14400

gccagaatga ctgaaatctg tgcttttctg tgtgttgcag catcatgagc ggaagcggct 14460

cctttgaggg aggggtattc agcccttatc tgacggggcg tctcccctcc tgggcgggag 14520

tgcgtcagaa tgtgatggga tccacggtgg acggccggcc cgtgcagccc gcgaactctt 14580

caaccctgac ctatgcaacc ctgagctctt cgtcggtgga cgcagctgcc gccgcagctg 14640

ctgcatccgc cgccagcgcc gtgcgcggaa tggccatggg cgccggctac tacggcactc 14700

tggtggccaa ctcgagttcc accaataatc ccgccagcct gaacgaggag aagctgctgc 14760

tgctgatggc ccagcttgag gccttgaccc agcgcctggg cgagctgacc cagcaggtgg 14820

ctcagctgca ggagcagacg cgggccgcgg ttgccacggt gaaatccaaa taaaaaatga 14880

atcaataaat aaacggagac ggttgttgat tttaacacag agtctgaatc tttatttgat 14940

ttttcgcgcg cggtaggccc tggaccaccg gtctcgatca ttgagcaccc ggtggatctt 15000

ttccaggacc cggtagaggt gggcttggat gttgaggtac atgggcatga gcccgtcccg 15060

ggggtggagg tagctccatt gcagggcctc gtgctcgggg gtggtgttgt aaatcaccca 15120

gtcatagcag gggcgcaggg cgtggtgttg cacaatatct ttgaggagga gactgatggc 15180

cacgggcagc cctttggtgt aggtgtttac aaatctgttg agctgggagg gatgcatgcg 15240

gggggagatg aggtgcatct tggcctggat cttgagattg gcgatgttac cgcccagatc 15300

ccgcctgggg ttcatgttgt gcaggaccac cagcacggtg tatccggtgc acttggggaa 15360

tttatcatgc aacttggaag ggaaggcgtg aaagaatttg gcgacgccct tgtgtccgcc 15420

caggttttcc atgcactcat ccatgatgat ggcaatgggc ccgtgggcgg cggcctgggc 15480

aaagacgttt cgggggtcgg acacatcata gttgtggtcc tgggtgaggt catcataggc 15540

cattttaatg aatttggggc ggagggtgcc ggactggggg acaaaggtac cctcgatccc 15600

gggggcgtag ttcccctcac agatctgcat ctcccaggct ttgagctcag agggggggat 15660

catgtccacc tgcggggcga taaagaacac ggtttccggg gcgggggaga tgagctgggc 15720

cgaaagcaag ttccggagca gctgggactt gccgcagccg gtggggccgt aaatgacccc 15780

gatgaccggc tgcaggtggt agttgaggga gagacagctg ccgtcctccc ggaggagggg 15840

ggccacctcg ttcatcatct cgcgcacgtg catgttctcg cgcaccagtt ccgccaggag 15900

gcgctctccc cccagagata ggagctcctg gagcgaggcg aagtttttca gcggcttgag 15960

tccgtcggcc atgggcattt tggagagggt ctgttgcaag agttccaagc ggtcccagag 16020

ctcggtgatg tgctctacgg catctcgatc cagcagacct cctcgtttcg cgggttggga 16080

cgactgcggg agtagggcac cagacgatgg gcgtccagcg cagccagggt ccggtccttc 16140

cagggccgca gcgtccgcgt cagggtggtc tccgtcacgg tgaaggggtg cgcgccgggc 16200

tgggcgcttg cgagggtgcg cttcaggctc atccggctgg tcgaaaaccg ctcccgatcg 16260

gcgccctgcg cgtcggccag gtagcaattg accatgagtt cgtagttgag cgcctcggcc 16320

gcgtggcctt tggcgcggag cttacctttg gaagtctgcc cgcaggcggg acagaggagg 16380

gacttgaggg cgtagagctt gggggcgagg aagacggaat cgggggcgta ggcgtccgcg 16440

ccgcagtggg cgcagacggt ctcgcactcc acgagccagg tgaggtcggg ctggtcgggg 16500

tcaaaaacca gtttcccgcc gttctttttg atgcgtttct tacctttggt ctccatgagc 16560

tcgtgtcccc gctgggtgac aaagaggctg tccgtgtccc cgtagaccga ctttatgggc 16620

cggtcctcga gcggtgtgcc gcggtcctcc tcgtagagga accccgccca ctccgagacg 16680

aaagcccggg tccaggccag cacgaaggag gccacgtggg acgggtagcg gtcgttgtcc 16740

accagcgggt ccactttttc cagggtatgc aaacacatgt ccccctcgtc cacatccagg 16800

aaggtgattg gcttgtaagt gtaggccacg tgaccggggg tcccggccgg gggggtataa 16860

aagggggcgg gcccctgctc gtcctcactg tcttccggat cgctgtccag gagcgccagc 16920

tgttggggta ggtattccct ctcgaaggcg ggcatgacct cggcactcag gttgtcagtt 16980

tctagaaacg aggaggattt gatattgacg gtgccagcgg agatgccttt caagagcccc 17040

tcgtccatct ggtcagaaaa gacgattttt ttgttgtcga gcttggtggc gaaggagccg 17100

tagagggcgt tggaaaggag cttggcgatg gagcgcatgg tctggttttt ttccttgtcg 17160

gcgcgctcct tggccgcgat gttgagctgc acgtactcgc gcgccacgca cttccattcg 17220

gggaagacgg tggtcatctc gtcgggcacg attctgacct gccaacctcg attatgcagg 17280

gtgatgaggt ccacactggt ggccacctcg ccgcgcaggg gctcgttggt ccagcagagg 17340

cggccgccct tgcgcgagca gaaggggggc agagggtcca gcatgacctc gtcggggggg 17400

tcggcatcga tggtgaagat gccgggcagg agatcggggt cgaagtagct gatggaagtg 17460

gccagatcgt ccagggaagc ttgccattcg cgcacggcca gcgcgcgctc gtagggactg 17520

aggggcgtgc cccagggcat ggggtgggtg agcgcggagg cgtacatgcc gcagatgtcg 17580

tagacgtaga ggggctcctc gaggatgccg atgtaggtgg ggtagcagcg ccccccgcgg 17640

atgctggcgc gcacgtagtc atacagctcg tgcgagggcg cgaggagccc cgggcccagg 17700

ttggtgcgac tgggcttttc ggcgcggtag acgatctggc gaaagatggc atgcgagttg 17760

gaggagatgg tgggcctttg gaagatgttg aagtgggcgt gggggaggcc gaccgagtcg 17820

cggatgaagt gggcgtagga gtcttgcagt ttggcgacga gctcggcggt gacgaggacg 17880

tccagagcgc agtagtcgag ggtctcctgg atgatgtcat acttgagctg gcccttttgt 17940

ttccacagct cgcggttgag aaggaactct tcgcggtcct tccagtactc ttcgaggggg 18000

aacccgtcct gatctgcacg gtaagagcct agcatgtaga actggttgac ggccttgtag 18060

gcgcagcagc ccttctccac ggggagggcg taggcctggg cggccttgcg cagggaggtg 18120

tgcgtgaggg cgaaggtgtc cctgaccatg accttgagga actggtgctt gaaatcgata 18180

tcgtcgcagc ccccctgctc ccagagctgg aagtccgtgc gcttcttgta ggcggggttg 18240

ggcaaagcga aagtaacatc gttgaaaagg atcttgcccg cgcggggcat aaagttgcga 18300

gtgatgcgga aaggctgggg cacctcggcc cggttgttga tgacctgggc ggcgagcacg 18360

atctcgtcga aaccgttgat gttgtggccc acgatgtaga gttccacgaa tcgcgggcgg 18420

cccttgacgt ggggcagctt cttgagctcc tcgtaggtga gctcgtcggg gtcgctgaga 18480

ccgtgctgct cgagcgccca gtcggcgaga tgggggttgg cgcggaggaa ggaagtccag 18540

agatccacgg ccagggcggt ttgcagacgg tcccggtact gacggaactg ctgcccgacg 18600

gccatttttt cgggggtgac gcagtagaag gtgcgggggt ccccgtgcca gcggtcccat 18660

ttgagctgga gggcgagatc gagggcgagc tcgacgaggc ggtcgtcccc tgagagtttc 18720

atgaccagca tgaaggggac gagctgcttg ccgaaggacc ccatccaggt gtaggtttcc 18780

acatcgtagg tgaggaagag cctttcggtg cgaggatgcg agccgatggg gaagaactgg 18840

atctcctgcc accaattgga ggaatggctg ttgatgtgat ggaagtagaa atgccgacgg 18900

cgcgccgaac actcgtgctt gtgtttatac aagcggccac agtgctcgca acgctgcacg 18960

ggatgcacgt gctgcacgag ctgtacctga gttcctttga cgaggaattt cagtgggaag 19020

tggagtcgtg gcgcctgcat ctcgtgctgt actacgtcgt ggtggtcggc ctggccctct 19080

tctgcctcga tggtggtcat gctgacgagc ccgcgcggga ggcaggtcca gacctcggcg 19140

cgagcgggtc ggagagcgag gacgagggcg cgcaggccgg agctgtccag ggtcctgaga 19200

cgctgcggag tcaggtcagt gggcagcggc ggcgcgcggt tgacttgcag gagtttttcc 19260

agggcgcgcg ggaggtccag atggtacttg atctccaccg cgccgttggt ggcgacgtcg 19320

atggcttgca gggtcccgtg cccctggggt gtgaccaccg tcccccgttt cttcttgggc 19380

ggctggggcg acgggggcgg tgcctcttcc atggttagaa gcggcggcga ggacgcgcgc 19440

cgggcggcag aggcggctcg gggcccggag gcaggggcgg caggggcacg tcggcgccgc 19500

gcgcgggtag gttctggtac tgcgcccgga gaagactggc gtgagcgacg acgcgacggt 19560

tgacgtcctg gatctgacgc ctctgggtga aggccacggg acccgtgagt ttgaacctga 19620

aagagagttc gacagaatca atctcggtat cgttgacggc ggcctgccgc aggatctctt 19680

gcacgtcgcc cgagttgtcc tggtaggcga tctcggtcat gaactgctcg atctcctcct 19740

cctgaaggtc tccgcgaccg gcgcgctcca cggtggccgc gaggtcgttg gagatgcggc 19800

ccatgagctg cgagaaggcg ttcatgcccg cctcgttcca gacgcggctg tagaccacga 19860

cgccctcggg atcgcgggcg cgcatgacca cctgggcgag gttgagctcc acgtggcgcg 19920

tgaagaccgc gtagttgcag aggcgctggt agaggtagtt gagcgtggtg gcgatgtgct 19980

cggtgacgaa gaaatacatg atccagcggc ggagcggcat ctcgctgacg tcgcccagcg 20040

cctccaagcg ttccatggcc tcgtaaaagt ccacggcgaa gttgaaaaac tgggagttgc 20100

gcgccgagac ggtcaactcc tcctccagaa gacggatgag ctcggcgatg gtggcgcgca 20160

cctcgcgctc gaaggccccc gggagttcct ccacttcctc ctcttcttcc tcctccacta 20220

acatctcttc tacttcctcc tcaggcggtg gtggtggcgg gggagggggc ctgcgtcgcc 20280

ggcggcgcac gggcagacgg tcgatgaagc gctcgatggt ctcgccgcgc cggcgtcgca 20340

tggtctcggt gacggcgcgc ccgtcctcgc ggggccgcag cgtgaagacg ccgccgcgca 20400

tctccaggtg gccggggggg tccccgttgg gcagggagag ggcgctgacg atgcatctta 20460

tcaattgccc cgtagggact ccgcgcaagg acctgagcgt ctcgagatcc acgggatctg 20520

aaaaccgttg aacgaaggct tcgagccagt cgcagtcgca aggtaggctg agcacggttt 20580

cttctgccgg gtcatgttgg ggagcggggc gggcgatgct gctggtgatg aagttgaaat 20640

aggcggttct gagacggcgg atggtggcga ggagcaccag gtctttgggc ccggcttgct 20700

ggatgcgcag acggtcggcc atgccccagg cgtggtcctg acacctggcc aggtccttgt 20760

agtagtcctg catgagccgc tccacgggca cctcctcctc gcccgcgcgg ccgtgcatgc 20820

gcgtgagccc gaagccgcgc tggggctgga cgagcgccag gtcggcgacg acgcgctcgg 20880

cgaggatggc ctgctggatc tgggtgaggg tggtctggaa gtcgtcaaag tcgacgaagc 20940

ggtggtaggc tccggtgttg atggtgtagg agcagttggc catgacggac cagttgacgg 21000

tctggtggcc cggacgcacg agctcgtggt acttgaggcg cgagtaggcg cgcgtgtcga 21060

agatgtagtc gttgcaggtg cgcaccaggt actggtagcc gatgaggaag tgcggcggcg 21120

gctggcggta gagcggccat cgctcggtgg cgggggcgcc gggcgcgagg tcctcgagca 21180

tggtgcggtg gtagccgtag atgtacctgg acatccaggt gatgccggcg gcggtggtgg 21240

aggcgcgcgg gaactcgcgg acgcggttcc agatgttgcg cagcggcagg aagtagttca 21300

tggtgggcac ggtctggccc gtgaggcgcg cgcagtcgtg gatgctctat acgggcaaaa 21360

acgaaagcgg tcagcggctc gactccgtgg cctggaggct aagcgaacgg gttgggctgc 21420

gcgtgtaccc cggttcgaat ctcgaatcag gctggagccg cagctaacgt ggtactggca 21480

ctcccgtctc gacccaagcc tgcaccaacc ctccaggata cggaggcggg tcgttttgca 21540

actttttttg gaggccggaa atgaaactag taagcgcgga aagcggccga ccgcgatggc 21600

tcgctgccgt agtctggaga agaatcgcca gggttgcgtt gcggtgtgcc ccggttcgag 21660

gccggccgga ttccgcggct aacgagggcg tggctgcccc gtcgtttcca agaccccata 21720

gccagccgac ttctccagtt acggagcgag cccctctttt gttttgtttg tttttgccag 21780

atgcatcccg tactgcggca gatgcgcccc caccaccctc caccgcaaca acagccccct 21840

cctccacagc cggcgcttct gcccccgccc cagcagcagc agcaacttcc agccacgacc 21900

gccgcggccg ccgtgagcgg ggctggacag acttctcagt atgatcacct ggccttggaa 21960

gagggcgagg ggctggcgcg cctgggggcg tcgtcgccgg agcggcaccc gcgcgtgcag 22020

atgaaaaggg acgctcgcga ggcctacgtg cccaagcaga acctgttcag agacaggagc 22080

ggcgaggagc ccgaggagat gcgcgcggcc cggttccacg cggggcggga gctgcggcgc 22140

ggcctggacc gaaagagggt gctgagggac gaggatttcg aggcggacga gctgacgggg 22200

atcagccccg cgcgcgcgca cgtggccgcg gccaacctgg tcacggcgta cgagcagacc 22260

gtgaaggagg agagcaactt ccaaaaatcc ttcaacaacc acgtgcgcac cctgatcgcg 22320

cgcgaggagg tgaccctggg cctgatgcac ctgtgggacc tgctggaggc catcgtgcag 22380

aaccccacca gcaagccgct gacggcgcag ctgttcctgg tggtgcagca tagtcgggac 22440

aacgaggcgt tcagggaggc gctgctgaat atcaccgagc ccgagggccg ctggctcctg 22500

gacctggtga acattctgca gagcatcgtg gtgcaggagc gcgggctgcc gctgtccgag 22560

aagctggcgg ccatcaactt ctcggtgctg agtctgggca agtactacgc taggaagatc 22620

tacaagaccc cgtacgtgcc catagacaag gaggtgaaga tcgacgggtt ttacatgcgc 22680

atgaccctga aagtgctgac cctgagcgac gatctggggg tgtaccgcaa cgacaggatg 22740

caccgcgcgg tgagcgccag caggcggcgc gagctgagcg accaggagct gatgcacagc 22800

ctgcagcggg ccctgaccgg ggccgggacc gagggggaga gctactttga catgggcgcg 22860

gacctgcact ggcagcccag ccgccgggcc ttggaggcgg caggcggtcc cccctacata 22920

gaagaggtgg acgatgaggt ggacgaggag ggcgagtacc tggaagactg atggcgcgac 22980

cgtatttttg ctagatgcaa caacagccac ctcctgatcc cgcgatgcgg gcggcgctgc 23040

agagccagcc gtccggcatt aactcctcgg acgattggac ccaggccatg caacgcatca 23100

tggcgctgac gacccgcaac cccgaagcct ttagacagca gccccaggcc aaccggctct 23160

cggccatcct ggaggccgtg gtgccctcgc gctccaaccc cacgcacgag aaggtcctgg 23220

ccatcgtgaa cgcgctggtg gagaacaagg ccatccgcgg cgacgaggcc ggcctggtgt 23280

acaacgcgct gctggagcgc gtggcccgct acaacagcac caacgtgcag accaacctgg 23340

accgcatggt gaccgacgtg cgcgaggccg tggcccagcg cgagcggttc caccgcgagt 23400

ccaacctggg atccatggtg gcgctgaacg ccttcctcag cacccagccc gccaacgtgc 23460

cccggggcca ggaggactac accaacttca tcagcgccct gcgcctgatg gtgaccgagg 23520

tgccccagag cgaggtgtac cagtccgggc cggactactt cttccagacc agtcgccagg 23580

gcttgcagac cgtgaacctg agccaggcgt tcaagaactt gcagggcctg tggggcgtgc 23640

aggccccggt cggggaccgc gcgacggtgt cgagcctgct gacgccgaac tcgcgcctgc 23700

tgctgctgct ggtggccccc ttcacggaca gcggcagcat caaccgcaac tcgtacctgg 23760

gctacctgat taacctgtac cgcgaggcca tcggccaggc gcacgtggac gagcagacct 23820

accaggagat cacccacgtg agccgcgccc tgggccagga cgacccgggc aatctggaag 23880

ccaccctgaa ctttttgctg accaaccggt cgcagaagat cccgccccag tacacgctca 23940

gcgccgagga ggagcgcatc ctgcgatacg tgcagcagag cgtgggcctg ttcctgatgc 24000

aggagggggc cacccccagc gccgcgctcg acatgaccgc gcgcaacatg gagcccagca 24060

tgtacgccag caaccgcccg ttcatcaata aactgatgga ctacttgcat cgggcggccg 24120

ccatgaactc tgactatttc accaacgcca tcctgaatcc ccactggctc ccgccgccgg 24180

ggttctacac gggcgagtac gacatgcccg accccaatga cgggttcctg tgggacgatg 24240

tggacagcag cgtgttctcc ccccgaccgg gtgctaacga gcgccccttg tggaagaagg 24300

aaggcagcga ccgacgcccg tcctcggcgc tgtccggccg cgagggtgct gccgcggcgg 24360

tgcccgaggc cgccagtcct ttcccgagct tgcccttctc gctgaacagt attcgcagca 24420

gcgagctggg caggatcacg cgcccgcgct tgctgggcga ggaggagtac ttgaatgact 24480

cgctgttgag acccgagcgg gagaagaact tccccaataa cgggatagag agcctggtgg 24540

acaagatgag ccgctggaag acgtatgcgc aggagcacag ggacgatccg tcgcaggggg 24600

ccacgagccg gggcagcgcc gcccgtaaac gccggtggca cgacaggcag cggggactga 24660

tgtgggacga tgaggattcc gccgacgaca gcagcgtgtt ggacttgggt gggagtggta 24720

acccgttcgc tcacctgcgc ccccgcatcg ggcgcatgat gtaagagaaa ccgaaaataa 24780

atgatactca ccaaggccat ggcgaccagc gtgcgttcgt ttcttctctg ttgttgtatc 24840

tagtatgatg aggcgtgcgt acccggaggg tcctcctccc tcgtacgaga gcgtgatgca 24900

gcaggcgatg gcggcggcgg cggcgatgca gcccccgctg gaggctcctt acgtgccccc 24960

gcggtacctg gcgcctacgg aggggcggaa cagcattcgt tactcggagc tggcaccctt 25020

gtacgatacc acccggttgt acctggtgga caacaagtcg gcggacatcg cctcgctgaa 25080

ctaccagaac gaccacagca acttcctgac caccgtggtg cagaacaatg acttcacccc 25140

cacggaggcc agcacccaga ccatcaactt tgacgagcgc tcgcggtggg gcggtcagct 25200

gaaaaccatc atgcacacca acatgcccaa cgtgaacgag ttcatgtaca gcaacaagtt 25260

caaggcgcgg gtgatggtct cccgcaagac ccccaacggg gtgacagtga cagatggtag 25320

tcaggatatc ttggagtatg aatgggtgga gtttgagctg cccgaaggca acttctcggt 25380

gaccatgacc atcgacctga tgaacaacgc catcatcgac aattacttgg cggtggggcg 25440

gcagaacggg gtcctggaga gcgatatcgg cgtgaagttc gacactagga acttcaggct 25500

gggctgggac cccgtgaccg agctggtcat gcccggggtg tacaccaacg aggccttcca 25560

ccccgatatt gtcttgctgc ccggctgcgg ggtggacttc accgagagcc gcctcagcaa 25620

cctgctgggc attcgcaaga ggcagccctt ccaggagggc ttccagatca tgtacgagga 25680

tctggagggg ggcaacatcc ccgcgctcct ggatgtcgac gcctatgaga aaagcaagga 25740

ggagagcgcc gccgcggcga ctgcagctgt agccaccgcc tctaccgagg tcaggggcga 25800

taattttgcc agccctgcag cagtggcagc ggccgaggcg gctgaaaccg aaagtaagat 25860

agtcattcag ccggtggaga aggatagcaa ggacaggagc tacaacgtgc tgccggacaa 25920

gataaacacc gcctaccgca gctggtacct ggcctacaac tatggcgacc ccgagaaggg 25980

cgtgcgctcc tggacgctgc tcaccacctc ggacgtcacc tgcggcgtgg agcaagtcta 26040

ctggtcgctg cccgacatga tgcaagaccc ggtcaccttc cgctccacgc gtcaagttag 26100

caactacccg gtggtgggcg ccgagctcct gcccgtctac tccaagagct tcttcaacga 26160

gcaggccgtc tactcgcagc agctgcgcgc cttcacctcg ctcacgcacg tcttcaaccg 26220

cttccccgag aaccagatcc tcgtccgccc gcccgcgccc accattacca ccgtcagtga 26280

aaacgttcct gctctcacag atcacgggac cctgccgctg cgcagcagta tccggggagt 26340

ccagcgcgtg accgttactg acgccagacg ccgcacctgc ccctacgtct acaaggccct 26400

gggcatagtc gcgccgcgcg tcctctcgag ccgcaccttc taaaaaatgt ccattctcat 26460

ctcgcccagt aataacaccg gttggggcct gcgcgcgccc agcaagatgt acggaggcgc 26520

tcgccaacgc tccacgcaac accccgtgcg cgtgcgcggg cacttccgcg ctccctgggg 26580

cgccctcaag ggccgcgtgc ggtcgcgcac caccgtcgac gacgtgatcg accaggtggt 26640

ggccgacgcg cgcaactaca cccccgccgc cgcgcccgtc tccaccgtgg acgccgtcat 26700

cgacagcgtg gtggccgacg cgcgccggta cgcccgcgcc aagagccggc ggcggcgcat 26760

cgcccggcgg caccggagca cccccgccat gcgcgcggcg cgagccttgc tgcgcagggc 26820

caggcgcacg ggacgcaggg ccatgctcag ggcggccaga cgcgcggctt caggcgccag 26880

cgccggcagg acccggagac gcgcggccac ggcggcggca gcggccatcg ccagcatgtc 26940

ccgcccgcgg cgagggaacg tgtactgggt gcgcgacgcc gccaccggtg tgcgcgtgcc 27000

cgtgcgcacc cgcccccctc gcacttgaag atgttcactt cgcgatgttg atgtgtccca 27060

gcggcgagga ggatgtccaa gcgcaaattc aaggaagaga tgctccaggt catcgcgcct 27120

gagatctacg gccccgcggt ggtgaaggag gaaagaaagc cccgcaaaat caagcgggtc 27180

aaaaaggaca aaaaggaaga agatgacgat ctggtggagt ttgtgcgcga gttcgccccc 27240

cggcggcgcg tgcagtggcg cgggcggaaa gtgcacccgg tgctgagacc cggcaccacc 27300

gtggtcttca cgcccggcga gcgctccggc agcgcttcca agcgctccta cgacgaggtg 27360

tacggggacg aggacatcct cgagcaggcg gccgagcgcc tgggcgagtt tgcttacggc 27420

aagcgcagcc gccccgccct gaaggaagag gcggtgtcca tcccgctgga ccacggcaac 27480

cccacgccga gcctcaagcc cgtgaccctg cagcaggtgc tgccgagcgc agcgccgcgc 27540

cgggggttca agcgcgaggg cgaggatctg taccccacca tgcagctgat ggtgcccaag 27600

cgccagaagc tggaagacgt gctggagacc atgaaggtgg acccggacgt gcagcccgag 27660

gtcaaggtgc ggcccatcaa gcaggtggcc ccgggcctgg gcgtgcagac cgtggacatc 27720

aagatcccca cggagcccat ggaaacgcag accgagccca tgatcaagcc cagcaccagc 27780

accatggagg tgcagacgga tccctggatg ccatcggctc ctagccgaag accccggcgc 27840

aagtacggcg cggccagcct gctgatgccc aactacgcgc tgcatccttc catcatcccc 27900

acgccgggct accgcggcac gcgcttctac cgcggtcata caaccagccg ccgccgcaag 27960

accaccaccc gccgccgccg tcgccgcaca gccgctgcat ctacccctgc cgccctggtg 28020

cggagagtgt accgccgcgg ccgcgcgcct ctgaccctac cgcgcgcgcg ctaccacccg 28080

agcatcgcca tttaaacttt cgcctgcttt gcagatggcc ctcacatgcc gcctccgcgt 28140

tcccattacg ggctaccgag gaagaaaacc gcgccgtaga aggctggcgg ggaacgggat 28200

gcgtcgccac caccatcggc ggcggcgcgc catcagcaag cggttggggg gaggcttcct 28260

gcccgcgctg atccccatca tcgccgcggc gatcggggcg atccccggca ttgcttccgt 28320

ggcggtgcag gcctctcagc gccactgaga cacttggaaa acatcttgta ataaaccaat 28380

ggactctgac gctcctggtc ctgtgatgtg ttttcgtaga cagatggaag acatcaattt 28440

ttcgtccctg gctccgcgac acggcacgcg gccgttcatg ggcacctgga gcgacatcgg 28500

caccagccaa ctgaacgggg gcgccttcaa ttggagcagt ctctggagcg ggcttaagaa 28560

tttcgggtcc acgcttaaaa cctatggcag caaggcgtgg aacagcacca cagggcaggc 28620

gctgagggat aagctgaaag agcagaactt ccagcagaag gtggtcgatg ggctcgcctc 28680

gggcatcaac ggggtggtgg acctggccaa ccaggccgtg cagcggcaga tcaacagccg 28740

cctggacccg gtgccgcccg ccggctccgt ggagatgccg caggtggagg aggagctgcc 28800

tcccctggac aagcggggcg agaagcgacc ccgccccgac gcggaggaga cgctgctgac 28860

gcacacggac gagccgcccc cgtacgagga ggcggtgaaa ctgggtctgc ccaccacgcg 28920

gcccatcgcg cccctggcca ccggggtgct gaaacccgaa agtaataagc ccgcgaccct 28980

ggacttgcct cctcccgctt cccgcccctc tacagtggct aagcccctgc cgccggtggc 29040

cgtggcccgc gcgcgacccg ggggctccgc ccgccctcat gcgaactggc agagcactct 29100

gaacagcatc gtgggtctgg gagtgcagag tgtgaagcgc cgccgctgct attaaaccta 29160

ccgtagcgct taacttgctt gtctgtgtgt gtatgtatta tgtcgccgct gtccgccaga 29220

aggaggagtg aagaggcgcg tcgccgagtt gcaagatggc caccccatcg atgctgcccc 29280

agtgggcgta catgcacatc gccggacagg acgcttcgga gtacctgagt ccgggtctgg 29340

tgcagttcgc ccgcgccaca gacacctact tcagtctggg gaacaagttt aggaacccca 29400

cggtggcgcc cacgcacgat gtgaccaccg accgcagcca gcggctgacg ctgcgcttcg 29460

tgcccgtgga ccgcgaggac aacacctact cgtacaaagt gcgctacacg ctggccgtgg 29520

gcgacaaccg cgtgctggac atggccagca cctactttga catccgcggc gtgctggatc 29580

ggggccctag cttcaaaccc tactccggca ccgcctacaa cagcctggct cccaagggag 29640

cgcccaattc cagccagtgg gagcaaaaaa aggcaggcaa tggtgacact atggaaacac 29700

acacatttgg tgtggcccca atgggcggtg agaatattac aatcgacgga ttacaaattg 29760

gaactgacgc tacagctgat caggataaac caatttatgc tgacaaaaca ttccagcctg 29820

aacctcaagt aggagaagaa aattggcaag aaactgaaag cttttatggc ggtagggctc 29880

ttaaaaaaga cacaagcatg aaaccttgct atggctccta tgctagaccc accaatgtaa 29940

agggaggtca agctaaactt aaagttggag ctgatggagt tcctaccaaa gaatttgaca 30000

tagacctggc tttctttgat actcccggtg gcacagtgaa tggacaagat gagtataaag 30060

cagacattgt catgtatacc gaaaacacgt atctggaaac tccagacacg catgtggtat 30120

acaaaccagg caaggatgat gcaagttctg aaattaacct ggttcagcag tccatgccca 30180

atagacccaa ctatattggg ttcagagaca actttattgg gctcatgtat tacaacagta 30240

ctggcaatat gggggtgctg gctggtcagg cctcacagct gaatgctgtg gtcgacttgc 30300

aagacagaaa caccgagctg tcataccagc tcttgcttga ctctttgggt gacagaaccc 30360

ggtatttcag tatgtggaat caggcggtgg acagttatga tcctgatgtg cgcattattg 30420

aaaaccatgg tgtggaagac gaacttccca actattgctt ccccctggat gggtctggca 30480

ctaatgccgc ttaccaaggt gtgaaagtaa aaaatggtaa cgatggtgat gttgagagcg 30540

aatgggaaaa tgatgatact gtcgcagctc gaaatcaatt atgcaagggc aacatttttg 30600

ccatggaaat taacctccaa gccaacctgt ggagaagttt cctctactcg aacgtggccc 30660

tgtacctgcc cgactcttac aagtacacgc cagccaacat caccctgccc accaacacca 30720

acacttatga ttacatgaac gggagagtgg tgcctccctc gctggtggac gcctacatca 30780

acatcggggc gcgctggtcg ctggacccca tggacaacgt caatcccttc aaccaccacc 30840

gcaacgcggg cctgcgctac cgctccatgc tcctgggcaa cgggcgctac gtgcccttcc 30900

acatccaggt gccccagaaa tttttcgcca tcaagagcct cctgctcctg cccgggtcct 30960

acacctacga gtggaacttc cgcaaggacg tcaacatgat cctgcagagc tccctcggca 31020

acgacctgcg cacggacggg gcctccatct ccttcaccag catcaacctc tacgccacct 31080

tcttccccat ggcgcacaac acggcctcca cgctcgaggc catgctgcgc aacgacacca 31140

acgaccagtc cttcaacgac tacctctcgg cggccaacat gctctacccc atcccggcca 31200

acgccaccaa cgtgcccatc tccatcccct cgcgcaactg ggccgccttc cgcggctggt 31260

ccttcacgcg cctcaagacc aaggagacgc cctcgctggg ctccgggttc gacccctact 31320

tcgtctactc gggctccatc ccctacctcg acggcacctt ctacctcaac cacaccttca 31380

agaaggtctc catcaccttc gactcctccg tcagctggcc cggcaacgac cggctcctga 31440

cgcccaacga gttcgaaatc aagcgcaccg tcgacggcga gggatacaac gtggcccagt 31500

gcaacatgac caaggactgg ttcctggtcc agatgctggc ccactacaac atcggctacc 31560

agggcttcta cgtgcccgag ggctacaagg accgcatgta ctccttcttc cgcaacttcc 31620

agcccatgag ccgccaggtg gtggacgagg tcaactacaa ggactaccag gccgtcaccc 31680

tggcctacca gcacaacaac tcgggcttcg tcggctacct cgcgcccacc atgcgccagg 31740

gccagcccta ccccgccaac tacccgtacc cgctcatcgg caagagcgcc gtcaccagcg 31800

tcacccagaa aaagttcctc tgcgacaggg tcatgtggcg catccccttc tccagcaact 31860

tcatgtccat gggcgcgctc accgacctcg gccagaacat gctctatgcc aactccgccc 31920

acgcgctaga catgaatttc gaagtcgacc ccatggatga gtccaccctt ctctatgttg 31980

tcttcgaagt cttcgacgtc gtccgagtgc accagcccca ccgcggcgtc atcgaggccg 32040

tctacctgcg cacccccttc tcggccggta acgccaccac ctaaattgct acttgcatga 32100

tggctgagcc cacaggctcc ggcgagcagg agctcagggc catcatccgc gacctgggct 32160

gcgggcccta cttcctgggc accttcgata agcgcttccc gggattcatg gccccgcaca 32220

agctggcctg cgccatcgtc aacacggccg gccgcgagac cgggggcgag cactggctgg 32280

ccttcgcctg gaacccgcgc tcgaacacct gctacctctt cgaccccttc gggttctcgg 32340

acgagcgcct caagcagatc taccagttcg agtacgaggg cctgctgcgc cgtagcgccc 32400

tggccaccga ggaccgctgc gtcaccctgg aaaagtccac ccagaccgtg cagggtccgc 32460

gctcggccgc ctgcgggctc ttctgctgca tgttcctgca cgccttcgtg cactggcccg 32520

accgccccat ggacaagaac cccaccatga acttgctgac gggggtgccc aacggcatgc 32580

tccagtcgcc ccaggtggaa cccaccctgc gccgcaacca ggaggcgctc taccgcttcc 32640

tcaactccca ctccgcctac tttcgctccc accgcgcgcg catcgagaag gccaccgcct 32700

tcgaccgcat gaacaatcaa gacatgtaaa ccgtgtgtgt atgtttaaaa tatcttttaa 32760

taaacagcac tttaatgtta cacatgcatc tgagatgatt ttattttaga aatcgaaagg 32820

gttctgccgg gtctcggcat ggcccgcggg cagggacacg ttgcggaact ggtacttggc 32880

cagccacttg aactcgggga tcagcagttt gggcagcggg gtgtcgggga aggagtcggt 32940

ccacagcttc cgcgtcagct gcagggcgcc cagcaggtcg ggcgcggaga tcttgaaatc 33000

gcagttggga cccgcgttct gcgcgcgaga gttgcggtac acggggttgc agcactggaa 33060

caccatcagg gccgggtgct tcacgctcgc cagcaccgcc gcgtcggtga tgctctccac 33120

gtcgaggtcc tcggcgttgg ccatcccgaa gggggtcatc ttgcaggtct gccttcccat 33180

ggtgggcacg cacccgggct tgtggttgca atcgcagtgc agggggatca gcatcatctg 33240

ggcctggtcg gcgttcatcc ccgggtacat ggccttcatg aaagcctcca attgcctgaa 33300

cgcctgctgg gccttggctc cctcggtgaa gaagaccccg caggacttgc tagagaactg 33360

gttggtggca cagccggcat cgtgcacgca gcagcgcgcg tcgttgttgg ccagctgcac 33420

cacgctgcgc ccccagcggt tctgggtgat cttggcccgg tcggggttct ccttcagcgc 33480

gcgctgcccg ttctcgctcg ccacatccat ctcgatcatg tgctccttct ggatcatggt 33540

ggtcccgtgc aggcaccgca gtttgccctc ggcctcggtg cacccgtgca gccacagcgc 33600

gcacccggtg cactcccagt tcttgtgggc gatctgggaa tgcgcgtgca cgaacccttg 33660

caggaagcgg cccatcatgg tcgtcagggt cttgttgcta gtgaaggtca acgggatgcc 33720

gcggtgctcc tcgttgatgt acaggtggca gatgcggcgg tacacctcgc cctgctcggg 33780

catcagttgg aagttggctt tcaggtcggt ctccacgcgg tagcggtcca tcagcatagt 33840

catgatttcc atgcccttct cccaggccga gacgatgggc aggctcatag ggttcttcac 33900

catcatctta gcactagcag ccgcggccag ggggtcgctc tcatccaggg tctcaaagct 33960

ccgcttgccg tccttctcgg tgatccgcac cggggggtag ctgaagccca cggccgccag 34020

ctcctcctcg gcctgtcttt cgtcctcgct gtcctggctg acgtcctgca tgaccacatg 34080

cttggtcttg cggggtttct tcttgggcgg cagtggcggc ggagatgctt gtggcgaggg 34140

ggagcgcgag ttctcgctca ccactactat ctcttcctct tcttggtccg aggccacgcg 34200

gcggtaggta tgtctcttcg ggggcagagg cggaggcgac gggctctcgc cgccgcgact 34260

tggcggatgg ctggcagagc cccttccgcg ttcgggggtg cgctcccggc ggcgctctga 34320

ctgacttcct ccgcggccgg ccattgtgtt ctcctaggga ggaacaacaa gcatggagac 34380

tcagccatcg ccaacctcgc catctgcccc caccgccggc gacgagaagc agcagcagca 34440

gaatgaaagc ttaaccgccc cgccgcccag ccccgcctcc gacgcagccg cggtcccaga 34500

catgcaagag atggaggaat ccatcgagat tgacctgggc tatgtgacgc ccgcggagca 34560

tgaggaggag ctggcagtgc gctttcaatc gtcaagccag gaagataaag aacagccaga 34620

gcaggaagca gagaacgagc agagtcaggc tgggctcgag catggcgact acctccacct 34680

gagcggggag gaggacgcgc tcatcaagca tctggcccgg caggccacca tcgtcaagga 34740

cgcgctgctc gaccgcaccg aggtgcccct cagcgtggag gagctcagcc gcgcctacga 34800

gctcaacctc ttctcgccgc gcgtgccccc caagcgccag cccaacggca cctgcgagcc 34860

caacccccgc ctcaacttct acccggtctt cgcggtgccc gaggccctgg ccacctacca 34920

catctttttc aagaaccaaa agatccccgt ctcctgccgc gccaaccgca cccgcgccga 34980

cgccctcttc aacctgggtc ccggcgcccg cctacctgat atcgcctcct tggaagaggt 35040

tcccaagatc ttcgagggtc tgggcagcga cgagactcgg gccgcgaacg ctctgcaagg 35100

agaaggagga ggagagcatg agcaccacag cgccctggtc gagttggaag gcgacaacgc 35160

gcggctggcg gtgctcaaac gcacggtcga gctgacccat ttcgcctacc cggctctgaa 35220

cctgcccccg aaagtcatga gcgcggtcat ggaccaggtg ctcatcaagc gcgcgtcgcc 35280

catctccgag gacgagggca tgcaagactc cgaggagggc aagcccgtgg tcagcgacga 35340

gcagctggcc cggtggctgg gtcctaatgc tacccctcaa agtttggaag agcggcgcaa 35400

gctcatgatg gccgtggtcc tggtgaccgt ggagctggag tgcctgcgcc gcttcttcgc 35460

cgacgcggag accctgcgca aggtcgagga gaacctgcac tacctcttca ggcacgggtt 35520

cgtgcgccag gcctgcaaga tctccaacgt ggagctgacc aacctggtct cctacatggg 35580

catcttgcac gagaaccgcc tggggcagaa cgtgctgcac accaccctgc gcggggaggc 35640

ccgccgcgac tacatccgcg actgcgtcta cctctacctc tgccacacct ggcagacggg 35700

catgggcgtg tggcagcagt gtctggagga gcagaacctg aaagagctct gcaagctcct 35760

gcaaaagaac ctcaagggtc tgtggaccgg gttcgacgag cggaccaccg cctcggacct 35820

ggccgacctc atcttccccg agcgcctcag gctgacgctg cgcaacggcc tgcccgactt 35880

tatgagccaa agcatgttgc aaaactttcg ctctttcatc ctcgaacgct ccggaatcct 35940

gcccgccacc tgctccgcgc tgccctcgga cttcgtgccg ctgaccttcc gcgagtgccc 36000

cccgccgctg tggagccact gctacctgct gcgcctggcc aactacctgg cctaccactc 36060

ggacgtgatc gaggacgtca gcggcgaggg cctgctcgag tgccactgcc gctgcaacct 36120

ctgcacgccg caccgctccc tggcctgcaa cccccagctg ctgagcgaga cccagatcat 36180

cggcaccttc gagttgcaag ggcccagcga gggcgaggga gccaaggggg gtctgaaact 36240

caccccgggg ctgtggacct cggcctactt gcgcaagttc gtgcccgagg attaccatcc 36300

cttcgagatc aggttctacg aggaccaatc ccagccgccc aaggccgagc tgtcggcctg 36360

cgtcatcacc cagggggcga tcctggccca attgcaagcc atccagaaat cccgccaaga 36420

attcttgctg aaaaagggcc gcggggtcta cctcgacccc cagaccggtg aggagctcaa 36480

ccccggcttc ccccaggatg ccccgaggaa acaagaagct gaaagtggag ctgccgcccg 36540

tggaggattt ggaggaagac tgggagaaca gcagtcaggc agaggagatg gaggaagact 36600

gggacagcac tcaggcagag gaggacagcc tgcaagacag tctggaggaa gacgaggagg 36660

aggcagagga ggaggtggaa gaagcagccg ccgccagacc gtcgtcctcg gcgggggaga 36720

aagcaagcag cacggatacc atctccgctc cgggtcgggg tcccgctcgg ccccacagta 36780

gatgggacga gaccgggcga ttcccgaacc ccaccaccca gaccggtaag aaggagcggc 36840

agggatacaa gtcctggcgg gggcacaaaa acgccatcgt ctcctgcttg caggcctgcg 36900

ggggcaacat ctccttcacc cggcgctacc tgctcttcca ccgcggggtg aacttccccc 36960

gcaacatctt gcattactac cgtcacctcc acagccccta ctacttccaa gaagaggcag 37020

cagcagcaga aaaagaccag aaaaccagct agaaaatcca cagcggcggc agcggcaggt 37080

ggactgagga tcgcggcgaa cgagccggcg cagacccggg agctgaggaa ccggatcttt 37140

cccaccctct atgccatctt ccagcagagt cgggggcagg agcaggaact gaaagtcaag 37200

aaccgttctc tgcgctcgct cacccgcagt tgtctgtatc acaagagcga agaccaactt 37260

cagcgcactc tcgaggacgc cgaggctctc ttcaacaagt actgcgcgct cactcttaaa 37320

gagtagcccg cgcccgccca gtcgcagaaa aaggcgggaa ttacgtcacc tgtgcccttc 37380

gccctagccg cctccaccca gcaccgccat gagcaaagag attcccacgc cttacatgtg 37440

gagctaccag ccccagatgg gcctggccgc cggcgccgcc caggactact ccacccgcat 37500

gaattggctc agcgccgggc ccgcgatgat ctcacgggtg aatgacatcc gcgcccaccg 37560

aaaccagata ctcctagaac agtcagcgct caccgccacg ccccgcaatc acctcaatcc 37620

gcgtaattgg cccgccgccc tggtgtacca ggaaattccc cagcccacga ccgtactact 37680

tccgcgagac gcccaggccg aagtccagct gactaactca ggtgtccagc tggcgggcgg 37740

cgccaccctg tgtcgtcacc gccccgctca gggtataaag cggctggtga tccggggcag 37800

aggcacacag ctcaacgacg aggtggtgag ctcttcgctg ggtctgcgac ctgacggagt 37860

cttccaactc gccggatcgg ggagatcttc cttcacgcct cgtcaggcgg tcctgacttt 37920

ggagagttcg tcctcgcagc cccgctcggg cggcatcggc actctccagt tcgtggagga 37980

gttcactccc tcggtctact tcaacccctt ctccggctcc cccggccact acccggacga 38040

gttcatcccg aactttgacg ccatcagcga gtcggtggac ggctacgatt gattaattaa 38100

tcaactaacc ccttacccct ttaccctcca gtaaaaataa agattaaaaa tgattgaatt 38160

gatcaataaa gaatcactta cttgaaatct gaaaccaggt ctctgtccat gttttctgtc 38220

agcagcactt cactcccctc ttcccaactc tggtactgca ggccccggcg ggctgcaaac 38280

ttcctccaca ctctgaaggg gatgtcaaat tcctcctgtc cctcaatctt catttttatc 38340

ttctatcaga tgtccaaaaa gcgcgcgcgg gtggatgatg gcttcgaccc cgtgtacccc 38400

tacgatgcag acaacgcacc gactgtgccc ttcatcaacc ctcccttcgt ctcttcagat 38460

ggattccaag aaaagcccct gggggtgttg tccctgcgac tggccgaccc cgtcaccacc 38520

aagaatgggg ctgtcaccct caagctgggg gagggggtgg acctcgacga ctcgggaaaa 38580

ctcatctcca aaaatgccac caaggccact gcccctctca gtatttccaa cggcaccatt 38640

tcccttaaca tggctgcccc tttttacaac aacaatggaa cgttaagtct caatgtttct 38700

acaccattag cagtatttcc cacttttaac actttaggta tcagtcttgg aaacggtctt 38760

caaacttcta ataagttgct gactgtacag ttaactcatc ctcttacatt cagctcaaat 38820

agcatcacag taaaaacaga caaaggactc tatattaatt ctagtggaaa cagagggctt 38880

gaggctaaca taagcctaaa aagaggactg atttttgatg gtaatgctat tgcaacatac 38940

cttggaagtg gtttagacta tggatcctat gatagcgatg ggaaaacaag acccatcatc 39000

accaaaattg gagcaggttt gaattttgat gctaataatg ccatggctgt gaagctaggc 39060

acaggtttaa gttttgactc tgccggtgcc ttaacagctg gaaacaaaga ggatgacaag 39120

ctaacacttt ggactacacc tgacccaagc cctaattgtc aattactttc agacagagat 39180

gccaaattta ccctatgtct tacaaaatgc ggtagtcaaa tactaggcac tgttgcagta 39240

gctgctgtta ctgtaggttc agcactaaat ccaattaatg acacagtaaa aagcgccata 39300

gtattcctta gatttgactc tgacggtgtg ctcatgtcaa actcatcaat ggtaggtgat 39360

tactggaact ttagggaagg acagaccacc caaagtgtgg cctatacaaa tgctgtggga 39420

ttcatgccca atctaggtgc atatcctaaa acccaaagca aaacaccaaa aaatagtata 39480

gtaagtcagg tatatttaaa tggagaaact actatgccaa tgacactgac aataactttc 39540

aatggcactg atgaaaaaga cacaacacct gtgagcactt actccatgac ttttacatgg 39600

cagtggactg gagactataa ggacaagaat attacctttg ctaccaactc ctttactttc 39660

tcctacatgg cccaagaata aaccctgcat gccaacccca ttgttcccac cactatggaa 39720

aactctgaag cagaaaaaaa taaagttcaa gtgttttatt gattcaacag ttttctcaca 39780

gaaccctagt attcaacctg ccacctccct cccaacacac agagtacaca gtcctttctc 39840

cccggctggc cttaaaaagc atcatatcat gggtaacaga catattctta ggtgttatat 39900

tccacacggt ttcctgtcga gccaaacgct catcagtgat attaataaac tccccgggca 39960

gctcacttaa gttcatgtcg ctgtccagct gctgagccac aggctgctgt ccaacttgcg 40020

gttgcttaac gggcggcgaa ggagaagtcc acgcctacat gggggtagag tcataatcgt 40080

gcatcaggat agggcggtgg tgctgcagca gcgcgcgaat aaactgctgc cgccgccgct 40140

ccgtcctgca ggaatacaac atggcagtgg tctcctcagc gatgattcgc accgcccgca 40200

gcataaggcg ccttgtcctc cgggcacagc agcgcaccct gatctcactt aaatcagcac 40260

agtaactgca gcacagcacc acaatattgt tcaaaatccc acagtgcaag gcgctgtatc 40320

caaagctcat ggcggggacc acagaaccca cgtggccatc ataccacaag cgcaggtaga 40380

ttaagtggcg acccctcata aacacgctgg acataaacat tacctctttt ggcatgttgt 40440

aattcaccac ctcccggtac catataaacc tctgattaaa catggcgcca tccaccacca 40500

tcctaaacca gctggccaaa acctgcccgc cggctataca ctgcagggaa ccgggactgg 40560

aacaatgaca gtggagagcc caggactcgt aaccatggat catcatgctc gtcatgatat 40620

caatgttggc acaacacagg cacacgtgca tacacttcct caggattaca agctcctccc 40680

gcgttagaac catatcccag ggaacaaccc attcctgaat cagcgtaaat cccacactgc 40740

agggaagacc tcgcacgtaa ctcacgttgt gcattgtcaa agtgttacat tcgggcagca 40800

gcggatgatc ctccagtatg gtagcgcggg tttctgtctc aaaaggaggt agacgatccc 40860

tactgtacgg agtgcgccga gacaaccgag atcgtgttgg tcgtagtgtc atgccaaatg 40920

gaacgccgga cgtagtcata tttcctgaag caaaaccagg tgcgggcgtg acaaacagat 40980

ctgcgtctcc ggtctcgccg cttagatcgc tctgtgtagt agttgtagta tatccactct 41040

ctcaaagcat ccaggcgccc cctggcttcg ggttctatgt aaactccttc atgcgccgct 41100

gccctgataa catccaccac cgcagaataa gccacaccca gccaacctac acattcgttc 41160

tgcgagtcac acacgggagg agcgggaaga gctggaagaa ccatgattaa ctttattcca 41220

aacggtctcg gagcacttca aaatgcaggt cccggaggtg gcacctctcg cccccactgt 41280

gttggtggaa aataacagcc aggtcaaagg tgacacggtt ctcgagatgt tccacggtgg 41340

cttccagcaa agcctccacg cgcacatcca gaaacaagag gacagcgaaa gcgggagcgt 41400

tttctaattc ctcaatcatc atattacact cctgcaccat ccccagataa ttttcatttt 41460

tccagccttg aatgattcgt attagttcct gaggtaaatc caagccagcc atgataaaaa 41520

gctcgcgcag agcgccctcc accggcattc ttaagcacac cctcataatt ccaagagatt 41580

ctgctcctgg ttcacctgca gcagattaac aatgggaata tcaaaatctc tgccgcgatc 41640

cctaagctcc tccctcaaca ataactgtat gtaatctttc atatcatctc cgaaattttt 41700

agccataggg ccgccaggaa taagagcagg gcaagccaca ttacagataa agcgaagtcc 41760

tccccagtgw gcattgccaa atgtaagatt gaaataagca tgctggctag accctgtgat 41820

atcttccaga taactggaca gaaaatcagg caagcaattt ttaagaaaat caacaaaaga 41880

aaagtcgtcc aggtgcaggt ttagagcctc aggaacaacg atggaataag tgcaaggagt 41940

gcgttccagc atggttagtg tttttttggt gatctgtaga acaaaaaata aacatgcaat 42000

attaaaccat gctagcctgg cgaacaggtg ggtaaatcac tctttccagc accaggcagg 42060

ctacggggtc tccggcgcga ccctcgtaga agctgtcgcc atgattgaaa agcatcaccg 42120

agagaccttc ccggtggccg gcatggatga ttcgagaaga agcatacact ccgggaacat 42180

tggcatccgt gagtgaaaaa aagcgaccta taaagcctcg gggcactaca atgctcaatc 42240

tcaattccag caaagccacc ccatgcggat ggagcacaaa attggcaggt gcgtaaaaaa 42300

tgtaattact cccctcctgc acaggcagca aagcccccgc tccctccaga aacacataca 42360

aagcctcagc gtccatagct taccgagcac ggcaggcgca agagtcagag aaaaggctga 42420

gctctaacct gactgcccgc tcctgtgctc aatatatagc cctaacctac actgacgtaa 42480

aggccaaagt ctaaaaatac ccgccaaaat gacacacacg cccagcacac gcccagaaac 42540

cggtgacaca ctcaaaaaaa tacgtgcgct tcctcaaacg cccaaaccgg cgtcatttcc 42600

gggttcccac gctacgtcac cgctcagcga ctttcaaatt ccgtcgaccg ttaaaaacgt 42660

cactcgcccc gcccctaacg gtcgcccttc tctcggccaa tcaccttcct cccttcccaa 42720

attcaaacgc ctcatttgca tattaacgcg cacaaaaagt ttgaggtata tatttgaatg 42780

atg 42783

<210> 63

<211> 32

<212> PRT

<213> 人工序列

<220>

<223> 肽佐剂

<400> 63

Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly

1 5 10 15

Ala Val Phe Val Ser Pro Ser Gln Glu Ile His Ala Arg Phe Arg Arg

20 25 30

<210> 64

<211> 27

<212> PRT

<213> 人工序列

<220>

<223> 肽佐剂

<400> 64

Ser Leu Leu Trp Gly Gly Val Thr Val Leu Ala Ala Met Leu Ile Ala

1 5 10 15

Gly Gln Val Ala Ser Ser Val Val Phe Leu Val

20 25

<210> 65

<211> 3849

<212> DNA

<213> 人工序列

<220>

<223> 疫苗构建体

<400> 65

gacgaggacg agacagccta cgacagcggc accgacctga tcgacttcat cgacgacagc 60

gacgagaatg agaacgactc cgacaccggc gaggacatgg tggatttcat cgacaacgac 120

gaaaccgacg aagagagcac cgagagcgac ctggacggct ttatcgacaa ctccgcccag 180

ctggctgaca gcgacagcaa tgcctgcgcc ttcctgaagg ctcagctggc agacgtgaac 240

agcaacgccg ctgcttttct gaagaactgc atcctgctgt acggcgctgc caacaccggc 300

aagagcctgt tcggcatgag cctgaactgc ctggtgctgt gcggcccagc caataccgga 360

aagtcctact tcggcatgtc cctgaattgt ctcgtgatct acggcccacc taacacaggc 420

aagtcctgct ttgccatgtc tctgtggccc tacctgcaca gcagactggt ggtgtttacc 480

ttccccaacc ccttctggcc ttacctggaa agccggatca ccgtgttcga gttccccaat 540

gcctttctga gatacctgca ctcccggatc cacgtgctgc agtttctgaa ccccttcaac 600

gtgtgccagg acaagatcct ggaacactac gagaacgaca gcaaggacat tctggaacat 660

tatgagaatg attccaagga cctgtgcgac cacatctgcg atcacatcga ctactggaag 720

cacatccggc tggaatgcgc catcatgtac aaggcccgga tcagactgga atgtgctatt 780

atgtataagg ctcgcgagat gggcttccac cagttcgacg gcgacatctg caacaccatg 840

cactacacca actggatcta tatctgcgag gacgcccagt gcaccgtggt ggaaggccag 900

gtggacaaga aatgggaggt gcacgctggc ggccaagtga tcctgtgtcc tgagagcggc 960

cagcggcgga tcaagaggcc cagaagcgag aactgccacc ccaacaagct gctgatcctg 1020

aagtgcctgc ggtacagatt caagaagcac tgcaagctga gcagcacctg gcactggacc 1080

tgccacgacg gcaagcacaa gtggcattgg acatgtcacg atgggaaaca caagaacgcc 1140

attgtgaccc tgacctacta cgaggccgac aagaacgacc tgaacgccca gatcgagcac 1200

tggaaactga tccggatgga atgtgcaatc ttctataagg ccaaagagct gggcatcagc 1260

atctgccacc aggtggtgcc tccactggcc gcctctaaag ccaaagcctg ccaggccatc 1320

gaactgcagc tggccctgga agccctgaat gccagcccct acgatgagtg gaccctgcag 1380

cagaccagcc tggaaatgtg gctggccgag ccccagttta agaagcacgg catcaccatc 1440

accgtgcagt acgacaatga caaggccaat accatggatt acacaaattg gaaagaaatc 1500

tacgtgatcg tgtgccccgc cagcatcccc tccgatgaga tcagcaccga ggaagccgac 1560

cacattgatt attggaaagc catcaggcag gaaaacgcca tcttcttcgc cgccagacac 1620

caggtggtgc ccgccctgaa tatctgcaag gccaaggcct gtaaagccat cgagtggaac 1680

accgagccca agcactgctt caagaagggc ggccagcaca tcgaagtgtg gttcgactac 1740

gtggcctggg acagcgtgta ctactgcggc gacgatggct ggtgcaagac cgaggccgag 1800

aagtacggct gcaagggcac ctgggaagtg catttcggca acagcatcga ctgcaacgac 1860

tccatgtgca gcaccttcga cgacaacgtg tccgccaccg agctcgtgaa ggaccatatc 1920

gactattgga agctgattcg cctggaatgt gccatttttt acaaggccag acggcggctg 1980

tccagcgacc aggatcagtc tcagctcgtg accaagtacc ccctgctgaa gctgctgtcc 2040

agacccccca acatgggcgt gaaggcccac ggcaagtgca tctgggagaa caaggtgttc 2100

atcgtgccca ccctgtgccc cgtgcctctg gatccaacat atcctctgct gaaactgctg 2160

accacccaga ccaccacccc cgagaatacc tccctggtgg aactgagagt gaccaccccc 2220

aagagcacag tcgtgatcag gctgcacctg accaccagat acccactgct gtcactgctg 2280

aacagctaca gcaccccccc tcaccggatc cctgctccat gtccttgggc tcctcagagg 2340

ccccccatcc ctaagccttc tccatgggcc cctagaatcc ctgccccttg cccctgggca 2400

cctcctagac ctccacactg tccatgggtg ccccctccac ctcctccaag accttgggcc 2460

ccttgcttcc tgctgtgctt ttgtgtgctg ctgtgcgtgt gcctgctgat cagacccctg 2520

ctgctgagtg tgtccaccta cctgaggcct ctgctgctgt ctatcagcgt gtacgctcag 2580

gtgctggtgc tggtgctgct gctgtgggtg tccatcggaa gcctgctgcc cagcgtgtgc 2640

atgtgtgcct atgcctgggt gctggtgttc gtgtacatcg tcgtgattac cagccccgcc 2700

accgccatcg tgtaccggga tggcaatcct tacgccgtgt gcgacaagtg cctgaagttc 2760

tacagcaaga tcagcgagta ccggcactac tgctacagcc tgtacggcac caccctggaa 2820

cagcagtaca acaagcccct gtgcgatctg ctgattcggt gcatcaacgt ggtgtacaga 2880

gactccatcc cccacgccgc ctgccacaag tgtatcgact tctactccag aatcagagag 2940

ctgcggcact acagcgactc cgtgtacggc gataccctgg aaaagctgac caacactggc 3000

ctgtacaacc tgctgattag atgcctgcgg gtgttctgca agaaggccct gacagccagc 3060

gaggtgtaca acttcgccta caccgatctg cgggtggtgt atcgggacag caaagtgcgg 3120

aagctgaggt actacaactg ctctgtgtat ggcgccagcc tggtgtattg caagggacag 3180

ctgaccgaga cagaggtgct ggatttcgcc ttcacagacc tgacaatcgt gtatcgcgac 3240

tccaaggtgt ccgagttccg gtggtacaga tattccgtgt atggcaccac actgtgcgtg 3300

gaatgcaaga aaaccctgca gagatctgag gtgtacgact gccagcggcc actgtgtccg 3360

caggaaaaga aaagacacgt ggacctgaac aagcggttcc acaccctgca cgagtacatg 3420

ctggatctgc agcccgagac aaccgacctg tactgctacg agcagcctga aaccactgat 3480

ctgcactgtt atgagcagct gggagacagc tccgatgaag aggacactgg cggcctggat 3540

ggggacgagg atgaggacga agtggaccat ctgcaggaac agccccagca ggctagacgg 3600

gacgaacagc acccttgcta tctgatcgag acacagtgct gcagatgcga atctctggtg 3660

gaagagaacg acgagatcga cggcgtgaac caccagcatc tgcccgctag aagggccgag 3720

cctcagagac acaccatgct gtgtatgtgc tgcaagtgcg aggccagaat cgccggctct 3780

ggacctggcg cctctggcaa gcctatcccc aatccactgc tgggcctgga ctccacccgg 3840

acctgataa 3849

<210> 66

<211> 1281

<212> PRT

<213> 人工序列

<220>

<223> 疫苗构建体

<400> 66

Asp Glu Asp Glu Thr Ala Tyr Asp Ser Gly Thr Asp Leu Ile Asp Phe

1 5 10 15

Ile Asp Asp Ser Asp Glu Asn Glu Asn Asp Ser Asp Thr Gly Glu Asp

20 25 30

Met Val Asp Phe Ile Asp Asn Asp Glu Thr Asp Glu Glu Ser Thr Glu

35 40 45

Ser Asp Leu Asp Gly Phe Ile Asp Asn Ser Ala Gln Leu Ala Asp Ser

50 55 60

Asp Ser Asn Ala Cys Ala Phe Leu Lys Ala Gln Leu Ala Asp Val Asn

65 70 75 80

Ser Asn Ala Ala Ala Phe Leu Lys Asn Cys Ile Leu Leu Tyr Gly Ala

85 90 95

Ala Asn Thr Gly Lys Ser Leu Phe Gly Met Ser Leu Asn Cys Leu Val

100 105 110

Leu Cys Gly Pro Ala Asn Thr Gly Lys Ser Tyr Phe Gly Met Ser Leu

115 120 125

Asn Cys Leu Val Ile Tyr Gly Pro Pro Asn Thr Gly Lys Ser Cys Phe

130 135 140

Ala Met Ser Leu Trp Pro Tyr Leu His Ser Arg Leu Val Val Phe Thr

145 150 155 160

Phe Pro Asn Pro Phe Trp Pro Tyr Leu Glu Ser Arg Ile Thr Val Phe

165 170 175

Glu Phe Pro Asn Ala Phe Leu Arg Tyr Leu His Ser Arg Ile His Val

180 185 190

Leu Gln Phe Leu Asn Pro Phe Asn Val Cys Gln Asp Lys Ile Leu Glu

195 200 205

His Tyr Glu Asn Asp Ser Lys Asp Ile Leu Glu His Tyr Glu Asn Asp

210 215 220

Ser Lys Asp Leu Cys Asp His Ile Cys Asp His Ile Asp Tyr Trp Lys

225 230 235 240

His Ile Arg Leu Glu Cys Ala Ile Met Tyr Lys Ala Arg Ile Arg Leu

245 250 255

Glu Cys Ala Ile Met Tyr Lys Ala Arg Glu Met Gly Phe His Gln Phe

260 265 270

Asp Gly Asp Ile Cys Asn Thr Met His Tyr Thr Asn Trp Ile Tyr Ile

275 280 285

Cys Glu Asp Ala Gln Cys Thr Val Val Glu Gly Gln Val Asp Lys Lys

290 295 300

Trp Glu Val His Ala Gly Gly Gln Val Ile Leu Cys Pro Glu Ser Gly

305 310 315 320

Gln Arg Arg Ile Lys Arg Pro Arg Ser Glu Asn Cys His Pro Asn Lys

325 330 335

Leu Leu Ile Leu Lys Cys Leu Arg Tyr Arg Phe Lys Lys His Cys Lys

340 345 350

Leu Ser Ser Thr Trp His Trp Thr Cys His Asp Gly Lys His Lys Trp

355 360 365

His Trp Thr Cys His Asp Gly Lys His Lys Asn Ala Ile Val Thr Leu

370 375 380

Thr Tyr Tyr Glu Ala Asp Lys Asn Asp Leu Asn Ala Gln Ile Glu His

385 390 395 400

Trp Lys Leu Ile Arg Met Glu Cys Ala Ile Phe Tyr Lys Ala Lys Glu

405 410 415

Leu Gly Ile Ser Ile Cys His Gln Val Val Pro Pro Leu Ala Ala Ser

420 425 430

Lys Ala Lys Ala Cys Gln Ala Ile Glu Leu Gln Leu Ala Leu Glu Ala

435 440 445

Leu Asn Ala Ser Pro Tyr Asp Glu Trp Thr Leu Gln Gln Thr Ser Leu

450 455 460

Glu Met Trp Leu Ala Glu Pro Gln Phe Lys Lys His Gly Ile Thr Ile

465 470 475 480

Thr Val Gln Tyr Asp Asn Asp Lys Ala Asn Thr Met Asp Tyr Thr Asn

485 490 495

Trp Lys Glu Ile Tyr Val Ile Val Cys Pro Ala Ser Ile Pro Ser Asp

500 505 510

Glu Ile Ser Thr Glu Glu Ala Asp His Ile Asp Tyr Trp Lys Ala Ile

515 520 525

Arg Gln Glu Asn Ala Ile Phe Phe Ala Ala Arg His Gln Val Val Pro

530 535 540

Ala Leu Asn Ile Cys Lys Ala Lys Ala Cys Lys Ala Ile Glu Trp Asn

545 550 555 560

Thr Glu Pro Lys His Cys Phe Lys Lys Gly Gly Gln His Ile Glu Val

565 570 575

Trp Phe Asp Tyr Val Ala Trp Asp Ser Val Tyr Tyr Cys Gly Asp Asp

580 585 590

Gly Trp Cys Lys Thr Glu Ala Glu Lys Tyr Gly Cys Lys Gly Thr Trp

595 600 605

Glu Val His Phe Gly Asn Ser Ile Asp Cys Asn Asp Ser Met Cys Ser

610 615 620

Thr Phe Asp Asp Asn Val Ser Ala Thr Glu Leu Val Lys Asp His Ile

625 630 635 640

Asp Tyr Trp Lys Leu Ile Arg Leu Glu Cys Ala Ile Phe Tyr Lys Ala

645 650 655

Arg Arg Arg Leu Ser Ser Asp Gln Asp Gln Ser Gln Leu Val Thr Lys

660 665 670

Tyr Pro Leu Leu Lys Leu Leu Ser Arg Pro Pro Asn Met Gly Val Lys

675 680 685

Ala His Gly Lys Cys Ile Trp Glu Asn Lys Val Phe Ile Val Pro Thr

690 695 700

Leu Cys Pro Val Pro Leu Asp Pro Thr Tyr Pro Leu Leu Lys Leu Leu

705 710 715 720

Thr Thr Gln Thr Thr Thr Pro Glu Asn Thr Ser Leu Val Glu Leu Arg

725 730 735

Val Thr Thr Pro Lys Ser Thr Val Val Ile Arg Leu His Leu Thr Thr

740 745 750

Arg Tyr Pro Leu Leu Ser Leu Leu Asn Ser Tyr Ser Thr Pro Pro His

755 760 765

Arg Ile Pro Ala Pro Cys Pro Trp Ala Pro Gln Arg Pro Pro Ile Pro

770 775 780

Lys Pro Ser Pro Trp Ala Pro Arg Ile Pro Ala Pro Cys Pro Trp Ala

785 790 795 800

Pro Pro Arg Pro Pro His Cys Pro Trp Val Pro Pro Pro Pro Pro Pro

805 810 815

Arg Pro Trp Ala Pro Cys Phe Leu Leu Cys Phe Cys Val Leu Leu Cys

820 825 830

Val Cys Leu Leu Ile Arg Pro Leu Leu Leu Ser Val Ser Thr Tyr Leu

835 840 845

Arg Pro Leu Leu Leu Ser Ile Ser Val Tyr Ala Gln Val Leu Val Leu

850 855 860

Val Leu Leu Leu Trp Val Ser Ile Gly Ser Leu Leu Pro Ser Val Cys

865 870 875 880

Met Cys Ala Tyr Ala Trp Val Leu Val Phe Val Tyr Ile Val Val Ile

885 890 895

Thr Ser Pro Ala Thr Ala Ile Val Tyr Arg Asp Gly Asn Pro Tyr Ala

900 905 910

Val Cys Asp Lys Cys Leu Lys Phe Tyr Ser Lys Ile Ser Glu Tyr Arg

915 920 925

His Tyr Cys Tyr Ser Leu Tyr Gly Thr Thr Leu Glu Gln Gln Tyr Asn

930 935 940

Lys Pro Leu Cys Asp Leu Leu Ile Arg Cys Ile Asn Val Val Tyr Arg

945 950 955 960

Asp Ser Ile Pro His Ala Ala Cys His Lys Cys Ile Asp Phe Tyr Ser

965 970 975

Arg Ile Arg Glu Leu Arg His Tyr Ser Asp Ser Val Tyr Gly Asp Thr

980 985 990

Leu Glu Lys Leu Thr Asn Thr Gly Leu Tyr Asn Leu Leu Ile Arg Cys

995 1000 1005

Leu Arg Val Phe Cys Lys Lys Ala Leu Thr Ala Ser Glu Val Tyr

1010 1015 1020

Asn Phe Ala Tyr Thr Asp Leu Arg Val Val Tyr Arg Asp Ser Lys

1025 1030 1035

Val Arg Lys Leu Arg Tyr Tyr Asn Cys Ser Val Tyr Gly Ala Ser

1040 1045 1050

Leu Val Tyr Cys Lys Gly Gln Leu Thr Glu Thr Glu Val Leu Asp

1055 1060 1065

Phe Ala Phe Thr Asp Leu Thr Ile Val Tyr Arg Asp Ser Lys Val

1070 1075 1080

Ser Glu Phe Arg Trp Tyr Arg Tyr Ser Val Tyr Gly Thr Thr Leu

1085 1090 1095

Cys Val Glu Cys Lys Lys Thr Leu Gln Arg Ser Glu Val Tyr Asp

1100 1105 1110

Cys Gln Arg Pro Leu Cys Pro Gln Glu Lys Lys Arg His Val Asp

1115 1120 1125

Leu Asn Lys Arg Phe His Thr Leu His Glu Tyr Met Leu Asp Leu

1130 1135 1140

Gln Pro Glu Thr Thr Asp Leu Tyr Cys Tyr Glu Gln Pro Glu Thr

1145 1150 1155

Thr Asp Leu His Cys Tyr Glu Gln Leu Gly Asp Ser Ser Asp Glu

1160 1165 1170

Glu Asp Thr Gly Gly Leu Asp Gly Asp Glu Asp Glu Asp Glu Val

1175 1180 1185

Asp His Leu Gln Glu Gln Pro Gln Gln Ala Arg Arg Asp Glu Gln

1190 1195 1200

His Pro Cys Tyr Leu Ile Glu Thr Gln Cys Cys Arg Cys Glu Ser

1205 1210 1215

Leu Val Glu Glu Asn Asp Glu Ile Asp Gly Val Asn His Gln His

1220 1225 1230

Leu Pro Ala Arg Arg Ala Glu Pro Gln Arg His Thr Met Leu Cys

1235 1240 1245

Met Cys Cys Lys Cys Glu Ala Arg Ile Ala Gly Ser Gly Pro Gly

1250 1255 1260

Ala Ser Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser

1265 1270 1275

Thr Arg Thr

1280

<210> 67

<211> 2661

<212> DNA

<213> 人工序列

<220>

<223> 病毒构建体

<400> 67

ttaattaacc atcttcaata atatacctca aactttttgt gcgcgttaat atgcaaatga 60

ggcgtttgaa tttggggagg aagggcggtg attggtcgag ggatgagcga ccgttagggg 120

cggggcgagt gacgttttga tgacgtggtt gcgaggagga gccagtttgc aagttctcgt 180

gggaaaagtg acgtcaaacg aggtgtggtt tgaacacgga aatactcaat tttcccgcgc 240

tctctgacag gaaatgaggt gtttctgggc ggatgcaagt gaaaacgggc cattttcgcg 300

cgaaaactga atgaggaagt gaaaatctga gtaatttcgc gtttatggca gggaggagta 360

tttgccgagg gccgagtaga ctttgaccga ttacgtgggg gtttcgatta ccgtgttttt 420

cacctaaatt tccgcgtacg gtgtcaaagt ccggtgtttt tacgcgatcg ctagcgacat 480

cgatcacaag tttgtacaaa aaagcaggct ccaccatggg aacccgcgtt ttgagatttc 540

tgtcgccgac taaattcatg tcgcgcgata gtggtgttta tcgccgatag agatggcgat 600

attggaaaaa tcgatatttg aaaatatggc atattgaaaa tgtcgccgat gtgagtttct 660

gtgtaactga tatcgccatt tttccaaaag tgatttttgg gcatacgcga tatctggcga 720

tagcgcttat atcgtttacg ggggatggcg atagacgact ttggtgactt gggcgattct 780

gtgtgtcgca aatatcgcag tttcgatata ggtgacagac gatatgaggc tatatcgccg 840

atagaggcga catcaagctg gcacatggcc aatgcatatc gatctataca ttgaatcaat 900

attggccatt agccatatta ttcattggtt atatagcata aatcaatatt ggctattggc 960

cattgcatac gttgtatcca tatcataata tgtacattta tattggctca tgtccaacat 1020

taccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat 1080

tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg 1140

gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa 1200

cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact 1260

tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta 1320

aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt 1380

acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg 1440

ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg 1500

ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc 1560

cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctcc 1620

ctatcagtga tagagatctc cctatcagtg atagagatcg tcgacgagct cgtttagtga 1680

accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg 1740

accgatccag cctccgcggc cgggaacggt gcattggaac gcggattccc cgtgccaaga 1800

gtgacgtaag taccgcctat agagtctata ggcccacccc cttggcttct tatgcatgct 1860

atactgtttt tggcttgggg tctatacacc cccgcttcct catgttatag gtgatggtat 1920

agcttagcct ataggtgtgg gttattgacc attattgacc actcccctat tggtgacgat 1980

actttccatt actaatccat aacatggctc tttgccacaa ctctctttat tggctatatg 2040

ccaatacact gtccttcaga gactgacacg gactctgtat ttttacagga tggggtctca 2100

tttattattt acaaattcac atatacaaca ccaccgtccc cagtgcccgc agtttttatt 2160

aaacataacg tgggatctcc acgcgaatct cgggtacgtg ttccggacat gggctcttct 2220

ccggtagcgg cggagcttct acatccgagc cctgctccca tgcctccagc gactcatggt 2280

cgctcggcag ctccttgctc ctaacagtgg aggccagact taggcacagc acgatgccca 2340

ccaccaccag tgtgccgcac aaggccgtgg cggtagggta tgtgtctgaa aatgagctcg 2400

gggagcgggc ttgcaccgct gacgcatttg gaagacttaa ggcagcggca gaagaagatg 2460

caggcagctg agttgttgtg ttctgataag agtcagaggt aactcccgtt gcggtgctgt 2520

taacggtgga gggcagtgta gtctgagcag tactcgttgc tgccgcgcgc gccaccagac 2580

ataatagctg acagactaac agactgttcc tttccatggg tcttttctgc agtcaccgtc 2640

cttgacacga agcttggtac c 2661

<210> 68

<211> 37490

<212> DNA

<213> 人工序列

<220>

<223> 病毒构建体

<400> 68

gcggccgctc gagcatgcat ctagagggcc ctattctata gtgtcaccta aatgctagag 60

ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc 120

ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg 180

aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg 240

acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta 300

tggcttctga ggcggaaaga accagctggg gctcgagggg ggatcgatcc cgtcgagata 360

tctagaccca gctttcttgt acaaagtggt gatcgattcg acagatcgcg atcgcaagtg 420

agtagtgttc tggggcgggg gaggacctgc atgagggcca gaataactga aatctgtgct 480

tttctgtgtg ttgcagcagc atgagcggaa gcggctcctt tgagggaggg gtattcagcc 540

cttatctgac ggggcgtctc ccctcctggg cgggagtgcg tcagaatgtg atgggatcca 600

cggtggacgg ccggcccgtg cagcccgcga actcttcaac cctgacctat gcaaccctga 660

gctcttcgtc gttggacgca gctgccgccg cagctgctgc atctgccgcc agcgccgtgc 720

gcggaatggc catgggcgcc ggctactacg gcactctggt ggccaactcg agttccacca 780

ataatcccgc cagcctgaac gaggagaagc tgttgctgct gatggcccag ctcgaggcct 840

tgacccagcg cctgggcgag ctgacccagc aggtggctca gctgcaggag cagacgcggg 900

ccgcggttgc cacggtgaaa tccaaataaa aaatgaatca ataaataaac ggagacggtt 960

gttgatttta acacagagtc tgaatcttta tttgattttt cgcgcgcggt aggccctgga 1020

ccaccggtct cgatcattga gcacccggtg gatcttttcc aggacccggt agaggtgggc 1080

ttggatgttg aggtacatgg gcatgagccc gtcccggggg tggaggtagc tccattgcag 1140

ggcctcgtgc tcgggggtgg tgttgtaaat cacccagtca tagcaggggc gcagggcatg 1200

gtgttgcaca atatctttga ggaggagact gatggccacg ggcagccctt tggtgtaggt 1260

gtttacaaat ctgttgagct gggagggatg catgcggggg gagatgaggt gcatcttggc 1320

ctggatcttg agattggcga tgttaccgcc cagatcccgc ctggggttca tgttgtgcag 1380

gaccaccagc acggtgtatc cggtgcactt ggggaattta tcatgcaact tggaagggaa 1440

ggcgtgaaag aatttggcga cgcctttgtg cccgcccagg ttttccatgc actcatccat 1500

gatgatggcg atgggcccgt gggcggcggc ctgggcaaag acgtttcggg ggtcggacac 1560

atcatagttg tggtcctggg tgaggtcatc ataggccatt ttaatgaatt tggggcggag 1620

ggtgccggac tgggggacaa aggtaccctc gatcccgggg gcgtagttcc cctcacagat 1680

ctgcatctcc caggctttga gctcggaggg ggggatcatg tccacctgcg gggcgataaa 1740

gaacacggtt tccggggcgg gggagatgag ctgggccgaa agcaagttcc ggagcagctg 1800

ggacttgccg cagccggtgg ggccgtagat gaccccgatg accggctgca ggtggtagtt 1860

gagggagaga cagctgccgt cctcccggag gaggggggcc acctcgttca tcatctcgcg 1920

cacgtgcatg ttctcgcgca ccagttccgc caggaggcgc tctcccccca gggataggag 1980

ctcctggagc gaggcgaagt ttttcagcgg cttgagtccg tcggccatgg gcattttgga 2040

gagggtttgt tgcaagagtt ccaggcggtc ccagagctcg gtgatgtgct ctacggcatc 2100

tcgatccagc agacctcctc gtttcgcggg ttgggacggc tgcgggagta gggcaccaga 2160

cgatgggcgt ccagcgcagc cagggtccgg tccttccagg gtcgcagcgt ccgcgtcagg 2220

gtggtctccg tcacggtgaa ggggtgcgcg ccgggctggg cgcttgcgag ggtgcgcttc 2280

aggctcatcc ggctggtcga aaaccgctcc cgatcggcgc cctgcgcgtc ggccaggtag 2340

caattgacca tgagttcgta gttgagcgcc tcggccgcgt ggcctttggc gcggagctta 2400

cctttggaag tctgcccgca ggcgggacag aggagggact tgagggcgta gagcttgggg 2460

gcgaggaaga cggactcggg ggcgtaggcg tccgcgccgc agtgggcgca gacggtctcg 2520

cactccacga gccaggtgag gtcgggctgg tcggggtcaa aaaccagttt cccgccgttc 2580

tttttgatgc gtttcttacc tttggtctcc atgagctcgt gtccccgctg ggtgacaaag 2640

aggctgtccg tgtccccgta gaccgacttt atgggccggt cctcgagcgg tgtgccgcgg 2700

tcctcctcgt agaggaaccc cgcccactcc gagacgaaag cccgggtcca ggccagcacg 2760

aaggaggcca cgtgggacgg gtagcggtcg ttgtccacca gcgggtccac cttttccagg 2820

gtatgcaaac acatgtcccc ctcgtccaca tccaggaagg tgattggctt gtaagtgtag 2880

gccacgtgac cgggggtccc ggccgggggg gtataaaagg gtgcgggtcc ctgctcgtcc 2940

tcactgtctt ccggatcgct gtccaggagc gccagctgtt ggggtaggta ttccctctcg 3000

aaggcgggca tgacctcggc actcaggttg tcagtttcta gaaacgagga ggatttgata 3060

ttgacggtgc cggcggagat gcctttcaag agcccctcgt ccatctggtc agaaaagacg 3120

atctttttgt tgtcgagctt ggtggcgaag gagccgtaga gggcgttgga gaggagcttg 3180

gcgatggagc gcatggtctg gtttttttcc ttgtcggcgc gctccttggc ggcgatgttg 3240

agctgcacgt actcgcgcgc cacgcacttc cattcgggga agacggtggt cagctcgtcg 3300

ggcacgattc tgacctgcca gccccgatta tgcagggtga tgaggtccac actggtggcc 3360

acctcgccgc gcaggggctc attagtccag cagaggcgtc cgcccttgcg cgagcagaag 3420

gggggcaggg ggtccagcat gacctcgtcg ggggggtcgg catcgatggt gaagatgccg 3480

ggcaggaggt cggggtcaaa gtagctgatg gaagtggcca gatcgtccag ggcagcttgc 3540

cattcgcgca cggccagcgc gcgctcgtag ggactgaggg gcgtgcccca gggcatggga 3600

tgggtaagcg cggaggcgta catgccgcag atgtcgtaga cgtagagggg ctcctcgagg 3660

atgccgatgt aggtggggta gcagcgcccc ccgcggatgc tggcgcgcac gtagtcatac 3720

agctcgtgcg agggggcgag gagccccggg cccaggttgg tgcgactggg cttttcggcg 3780

cggtagacga tctggcggaa aatggcatgc gagttggagg agatggtggg cctttggaag 3840

atgttgaagt gggcgtgggg cagtccgacc gagtcgcgga tgaagtgggc gtaggagtct 3900

tgcagcttgg cgacgagctc ggcggtgact aggacgtcca gagcgcagta gtcgagggtc 3960

tcctggatga tgtcatactt gagctgtccc ttttgtttcc acagctcgcg gttgagaagg 4020

aactcttcgc ggtccttcca gtactcttcg agggggaacc cgtcctgatc tgcacggtaa 4080

gagcctagca tgtagaactg gttgacggcc ttgtaggcgc agcagccctt ctccacgggg 4140

agggcgtagg cctgggcggc cttgcgcagg gaggtgtgcg tgagggcgaa agtgtccctg 4200

accatgacct tgaggaactg gtgcttgaag tcgatatcgt cgcagccccc ctgctcccag 4260

agctggaagt ccgtgcgctt cttgtaggcg gggttgggca aagcgaaagt aacatcgttg 4320

aagaggatct tgcccgcgcg gggcataaag ttgcgagtga tgcggaaagg ttggggcacc 4380

tcggcccggt tgttgatgac ctgggcggcg agcacgatct cgtcgaagcc gttgatgttg 4440

tggcccacga tgtagagttc cacgaatcgc ggacggccct tgacgtgggg cagtttcttg 4500

agctcctcgt aggtgagctc gtcggggtcg ctgagcccgt gctgctcgag cgcccagtcg 4560

gcgagatggg ggttggcgcg gaggaaggaa gtccagagat ccacggccag ggcggtttgc 4620

agacggtccc ggtactgacg gaactgctgc ccgacggcca ttttttcggg ggtgacgcag 4680

tagaaggtgc gggggtcccc gtgccagcga tcccatttga gctggagggc gagatcgagg 4740

gcgagctcga cgagccggtc gtccccggag agtttcatga ccagcatgaa ggggacgagc 4800

tgcttgccga aggaccccat ccaggtgtag gtttccacat cgtaggtgag gaagagcctt 4860

tcggtgcgag gatgcgagcc gatggggaag aactggatct cctgccacca attggaggaa 4920

tggctgttga tgtgatggaa gtagaaatgc cgacggcgcg ccgaacactc gtgcttgtgt 4980

ttatacaagc ggccacagtg ctcgcaacgc tgcacgggat gcacgtgctg cacgagctgt 5040

acctgagttc ctttgacgag gaatttcagt gggaagtgga gtcgtggcgc ctgcatctcg 5100

tgctgtacta cgtcgtggtg gtcggcctgg ccctcttctg cctcgatggt ggtcatgctg 5160

acgagcccgc gcgggaggca ggtccagacc tcggcgcgag cgggtcggag agcgaggacg 5220

agggcgcgca ggccggagct gtccagggtc ctgagacgct gcggagtcag gtcagtgggc 5280

agcggcggcg cgcggttgac ttgcaggagt ttttccaggg cgcgcgggag gtccagatgg 5340

tacttgatct ccaccgcgcc attggtggcg acgtcgatgg cttgcagggt cccgtgcccc 5400

tggggtgtga ccaccgtccc ccgtttcttc ttgggcggct ggggcgacgg gggcggtgcc 5460

tcttccatgg ttagaagcgg cggcgaggac gcgcgccggg cggcaggggc ggctcggggc 5520

ccggaggcag gggcggcagg ggcacgtcgg cgccgcgcgc gggtaggttc tggtactgcg 5580

cccggagaag actggcgtga gcgacgacgc gacggttgac gtcctggatc tgacgcctct 5640

gggtgaaggc cacgggaccc gtgagtttga acctgaaaga gagttcgaca gaatcaatct 5700

cggtatcgtt gacggcggcc tgccgcagga tctcttgcac gtcgcccgag ttgtcctggt 5760

aggcgatctc ggtcatgaac tgctcgatct cctcctcttg aaggtctccg cggccggcgc 5820

gctccacggt ggccgcgagg tcgttggaga tgcggcccat gagctgcgag aaggcgttca 5880

tgcccgcctc gttccagacg cggctgtaga ccacgacgcc ctcgggatcg ccggcgcgca 5940

tgaccacctg ggcgaggttg agctccacgt ggcgcgtgaa gaccgcgtag ttgcagaggc 6000

gctggtagag gtagttgagc gtggtggcga tgtgctcggt gacgaagaaa tacatgatcc 6060

agcggcggag cggcatctcg ctgacgtcgc ccagcgcctc caaacgttcc atggcctcgt 6120

aaaagtccac ggcgaagttg aaaaactggg agttgcgcgc cgagacggtc aactcctcct 6180

ccagaagacg gatgagctcg gcgatggtgg cgcgcacctc gcgctcgaag gcccccggga 6240

gttcctccac ttcctcttct tcctcctcca ctaacatctc ttctacttcc tcctcaggcg 6300

gcagtggtgg cgggggaggg ggcctgcgtc gccggcggcg cacgggcaga cggtcgatga 6360

agcgctcgat ggtctcgccg cgccggcgtc gcatggtctc ggtgacggcg cgcccgtcct 6420

cgcggggccg cagcgtgaag acgccgccgc gcatctccag gtggccgggg gggtccccgt 6480

tgggcaggga gagggcgctg acgatgcatc ttatcaattg ccccgtaggg actccgcgca 6540

aggacctgag cgtctcgaga tccacgggat ctgaaaaccg ctgaacgaag gcttcgagcc 6600

agtcgcagtc gcaaggtagg ctgagcacgg tttcttctgg cgggtcatgt tggttgggag 6660

cggggcgggc gatgctgctg gtgatgaagt tgaaataggc ggttctgaga cggcggatgg 6720

tggcgaggag caccaggtct ttgggcccgg cttgctggat gcgcagacgg tcggccatgc 6780

cccaggcgtg gtcctgacac ctggccaggt ccttgtagta gtcctgcatg agccgctcca 6840

cgggcacctc ctcctcgccc gcgcggccgt gcatgcgcgt gagcccgaag ccgcgctggg 6900

gctggacgag cgccaggtcg gcgacgacgc gctcggcgag gatggcttgc tggatctggg 6960

tgagggtggt ctggaagtca tcaaagtcga cgaagcggtg gtaggctccg gtgttgatgg 7020

tgtaggagca gttggccatg acggaccagt tgacggtctg gtggcccgga cgcacgagct 7080

cgtggtactt gaggcgcgag taggcgcgcg tgtcgaagat gtagtcgttg caggtgcgca 7140

ccaggtactg gtagccgatg aggaagtgcg gcggcggctg gcggtagagc ggccatcgct 7200

cggtggcggg ggcgccgggc gcgaggtcct cgagcatggt gcggtggtag ccgtagatgt 7260

acctggacat ccaggtgatg ccggcggcgg tggtggaggc gcgcgggaac tcgcggacgc 7320

ggttccagat gttgcgcagc ggcaggaagt agttcatggt gggcacggtc tggcccgtga 7380

ggcgcgcgca gtcgtggatg ctctatacgg gcaaaaacga aagcggtcag cggctcgact 7440

ccgtggcctg gaggctaagc gaacgggttg ggctgcgcgt gtaccccggt tcgaatctcg 7500

aatcaggctg gagccgcagc taacgtggta ttggcactcc cgtctcgacc caagcctgca 7560

ccaaccctcc aggatacgga ggcgggtcgt tttgcaactt ttttttggag gccggatgag 7620

actagtaagc gcggaaagcg gccgaccgcg atggctcgct gccgtagtct ggagaagaat 7680

cgccagggtt gcgttgcggt gtgccccggt tcgaggccgg ccggattccg cggctaacga 7740

gggcgtggct gccccgtcgt ttccaagacc ccatagccag ccgacttctc cagttacgga 7800

gcgagcccct cttttgtttt gtttgttttt gccagatgca tcccgtactg cggcagatgc 7860

gcccccacca ccctccaccg caacaacagc cccctccaca gccggcgctt ctgcccccgc 7920

cccagcagca acttccagcc acgaccgccg cggccgccgt gagcggggct ggacagagtt 7980

atgatcacca gctggccttg gaagagggcg aggggctggc gcgcctgggg gcgtcgtcgc 8040

cggagcggca cccgcgcgtg cagatgaaaa gggacgctcg cgaggcctac gtgcccaagc 8100

agaacctgtt cagagacagg agcggcgagg agcccgagga gatgcgcgcg gcccggttcc 8160

acgcggggcg ggagctgcgg cgcggcctgg accgaaagag ggtgctgagg gacgaggatt 8220

tcgaggcgga cgagctgacg gggatcagcc ccgcgcgcgc gcacgtggcc gcggccaacc 8280

tggtcacggc gtacgagcag accgtgaagg aggagagcaa cttccaaaaa tccttcaaca 8340

accacgtgcg caccctgatc gcgcgcgagg aggtgaccct gggcctgatg cacctgtggg 8400

acctgctgga ggccatcgtg cagaacccca ccagcaagcc gctgacggcg cagctgttcc 8460

tggtggtgca gcatagtcgg gacaacgaag cgttcaggga ggcgctgctg aatatcaccg 8520

agcccgaggg ccgctggctc ctggacctgg tgaacattct gcagagcatc gtggtgcagg 8580

agcgcgggct gccgctgtcc gagaagctgg cggccatcaa cttctcggtg ctgagtttgg 8640

gcaagtacta cgctaggaag atctacaaga ccccgtacgt gcccatagac aaggaggtga 8700

agatcgacgg gttttacatg cgcatgaccc tgaaagtgct gaccctgagc gacgatctgg 8760

gggtgtaccg caacgacagg atgcaccgtg cggtgagcgc cagcaggcgg cgcgagctga 8820

gcgaccagga gctgatgcat agtctgcagc gggccctgac cggggccggg accgaggggg 8880

agagctactt tgacatgggc gcggacctgc actggcagcc cagccgccgg gccttggagg 8940

cggcggcagg accctacgta gaagaggtgg acgatgaggt ggacgaggag ggcgagtacc 9000

tggaagactg atggcgcgac cgtatttttg ctagatgcaa caacaacagc cacctcctga 9060

tcccgcgatg cgggcggcgc tgcagagcca gccgtccggc attaactcct cggacgattg 9120

gacccaggcc atgcaacgca tcatggcgct gacgacccgc aaccccgaag cctttagaca 9180

gcagccccag gccaaccggc tctcggccat cctggaggcc gtggtgccct cgcgctccaa 9240

ccccacgcac gagaaggtcc tggccatcgt gaacgcgctg gtggagaaca aggccatccg 9300

cggcgacgag gccggcctgg tgtacaacgc gctgctggag cgcgtggccc gctacaacag 9360

caccaacgtg cagaccaacc tggaccgcat ggtgaccgac gtgcgcgagg ccgtggccca 9420

gcgcgagcgg ttccaccgcg agtccaacct gggatccatg gtggcgctga acgccttcct 9480

cagcacccag cccgccaacg tgccccgggg ccaggaggac tacaccaact tcatcagcgc 9540

cctgcgcctg atggtgaccg aggtgcccca gagcgaggtg taccagtccg ggccggacta 9600

cttcttccag accagtcgcc agggcttgca gaccgtgaac ctgagccagg ctttcaagaa 9660

cttgcagggc ctgtggggcg tgcaggcccc ggtcggggac cgcgcgacgg tgtcgagcct 9720

gctgacgccg aactcgcgcc tgctgctgct gctggtggcc cccttcacgg acagcggcag 9780

catcaaccgc aactcgtacc tgggctacct gattaacctg taccgcgagg ccatcggcca 9840

ggcgcacgtg gacgagcaga cctaccagga gatcacccac gtgagccgcg ccctgggcca 9900

ggacgacccg ggcaacctgg aagccaccct gaactttttg ctgaccaacc ggtcgcagaa 9960

gatcccgccc cagtacgcgc tcagcaccga ggaggagcgc atcctgcgtt acgtgcagca 10020

gagcgtgggc ctgttcctga tgcaggaggg ggccaccccc agcgccgcgc tcgacatgac 10080

cgcgcgcaac atggagccca gcatgtacgc cagcaaccgc ccgttcatca ataaactgat 10140

ggactacttg catcgggcgg ccgccatgaa ctctgactat ttcaccaacg ccatcctgaa 10200

tccccactgg ctcccgccgc cggggttcta cacgggcgag tacgacatgc ccgaccccaa 10260

tgacgggttc ctgtgggacg atgtggacag cagcgtgttc tccccccgac cgggtgctaa 10320

cgagcgcccc ttgtggaaga aggaaggcag cgaccgacgc ccgtcctcgg cgctgtccgg 10380

ccgcgagggt gctgccgcgg cggtgcccga ggccgccagt cctttcccga gcttgccctt 10440

ctcgctgaac agtatccgca gcagcgagct gggcaggatc acgcgcccgc gcttgctggg 10500

cgaagaggag tacttgaatg actcgctgtt gagacccgag cgggagaaga acttccccaa 10560

taacgggata gaaagcctgg tggacaagat gagccgctgg aagacgtatg cgcaggagca 10620

cagggacgat ccccgggcgt cgcagggggc cacgagccgg ggcagcgccg cccgtaaacg 10680

ccggtggcac gacaggcagc ggggacagat gtgggacgat gaggactccg ccgacgacag 10740

cagcgtgttg gacttgggtg ggagtggtaa cccgttcgct cacctgcgcc cccgtatcgg 10800

gcgcatgatg taagagaaac cgaaaataaa tgatactcac caaggccatg gcgaccagcg 10860

tgcgttcgtt tcttctctgt tgttgttgta tctagtatga tgaggcgtgc gtacccggag 10920

ggtcctcctc cctcgtacga gagcgtgatg cagcaggcga tggcggcggc ggcgatgcag 10980

cccccgctgg aggctcctta cgtgcccccg cggtacctgg cgcctacgga ggggcggaac 11040

agcattcgtt actcggagct ggcacccttg tacgatacca cccggttgta cctggtggac 11100

aacaagtcgg cggacatcgc ctcgctgaac taccagaacg accacagcaa cttcctgacc 11160

accgtggtgc agaacaatga cttcaccccc acggaggcca gcacccagac catcaacttt 11220

gacgagcgct cgcggtgggg cggccagctg aaaaccatca tgcacaccaa catgcccaac 11280

gtgaacgagt tcatgtacag caacaagttc aaggcgcggg tgatggtctc ccgcaagacc 11340

cccaatgggg tgacagtgac agaggattat gatggtagtc aggatgagct gaagtatgaa 11400

tgggtggaat ttgagctgcc cgaaggcaac ttctcggtga ccatgaccat cgacctgatg 11460

aacaacgcca tcatcgacaa ttacttggcg gtggggcggc agaacggggt gctggagagc 11520

gacatcggcg tgaagttcga cactaggaac ttcaggctgg gctgggaccc cgtgaccgag 11580

ctggtcatgc ccggggtgta caccaacgag gctttccatc ccgatattgt cttgctgccc 11640

ggctgcgggg tggacttcac cgagagccgc ctcagcaacc tgctgggcat tcgcaagagg 11700

cagcccttcc aggaaggctt ccagatcatg tacgaggatc tggagggggg caacatcccc 11760

gcgctcctgg atgtcgacgc ctatgagaaa agcaaggagg atgcagcagc tgaagcaact 11820

gcagccgtag ctaccgcctc taccgaggtc aggggcgata attttgcaag cgccgcagca 11880

gtggcagcgg ccgaggcggc tgaaaccgaa agtaagatag tcattcagcc ggtggagaag 11940

gatagcaaga acaggagcta caacgtacta ccggacaaga taaacaccgc ctaccgcagc 12000

tggtacctag cctacaacta tggcgacccc gagaagggcg tgcgctcctg gacgctgctc 12060

accacctcgg acgtcacctg cggcgtggag caagtctact ggtcgctgcc cgacatgatg 12120

caagacccgg tcaccttccg ctccacgcgt caagttagca actacccggt ggtgggcgcc 12180

gagctcctgc ccgtctactc caagagcttc ttcaacgagc aggccgtcta ctcgcagcag 12240

ctgcgcgcct tcacctcgct tacgcacgtc ttcaaccgct tccccgagaa ccagatcctc 12300

gtccgcccgc ccgcgcccac cattaccacc gtcagtgaaa acgttcctgc tctcacagat 12360

cacgggaccc tgccgctgcg cagcagtatc cggggagtcc agcgcgtgac cgttactgac 12420

gccagacgcc gcacctgccc ctacgtctac aaggccctgg gcatagtcgc gccgcgcgtc 12480

ctctcgagcc gcaccttcta aatgtccatt ctcatctcgc ccagtaataa caccggttgg 12540

ggcctgcgcg cgcccagcaa gatgtacgga ggcgctcgcc aacgctccac gcaacacccc 12600

gtgcgcgtgc gcgggcactt ccgcgctccc tggggcgccc tcaagggccg cgtgcggtcg 12660

cgcaccaccg tcgacgacgt gatcgaccag gtggtggccg acgcgcgcaa ctacaccccc 12720

gccgccgcgc ccgtctccac cgtggacgcc gtcatcgaca gcgtggtggc ggacgcgcgc 12780

cggtacgccc gcgccaagag ccggcggcgg cgcatcgccc ggcggcaccg gagcaccccc 12840

gccatgcgcg cggcgcgagc cttgctgcgc agggccaggc gcacgggacg cagggccatg 12900

ctcagggcgg ccagacgcgc ggcttcaggc gccagcgccg gcaggacccg gagacgcgcg 12960

gccacggcgg cggcagcggc catcgccagc atgtcccgcc cgcggcgagg gaacgtgtac 13020

tgggtgcgcg acgccgccac cggtgtgcgc gtgcccgtgc gcacccgccc ccctcgcact 13080

tgaagatgtt cacttcgcga tgttgatgtg tcccagcggc gaggaggatg tccaagcgca 13140

aattcaagga agagatgctc caggtcatcg cgcctgagat ctacggccct gcggtggtga 13200

aggaggaaag aaagccccgc aaaatcaagc gggtcaaaaa ggacaaaaag gaagaagaaa 13260

gtgatgtgga cggattggtg gagtttgtgc gcgagttcgc cccccggcgg cgcgtgcagt 13320

ggcgcgggcg gaaggtgcaa ccggtgctga gacccggcac caccgtggtc ttcacgcccg 13380

gcgagcgctc cggcaccgct tccaagcgct cctacgacga ggtgtacggg gatgatgata 13440

ttctggagca ggcggccgag cgcctgggcg agtttgctta cggcaagcgc agccgttccg 13500

caccgaagga agaggcggtg tccatcccgc tggaccacgg caaccccacg ccgagcctca 13560

agcccgtgac cttgcagcag gtgctgccga ccgcggcgcc gcgccggggg ttcaagcgcg 13620

agggcgagga tctgtacccc accatgcagc tgatggtgcc caagcgccag aagctggaag 13680

acgtgctgga gaccatgaag gtggacccgg acgtgcagcc cgaggtcaag gtgcggccca 13740

tcaagcaggt ggccccgggc ctgggcgtgc agaccgtgga catcaagatt cccacggagc 13800

ccatggaaac gcagaccgag cccatgatca agcccagcac cagcaccatg gaggtgcaga 13860

cggatccctg gatgccatcg gctcctagtc gaagaccccg gcgcaagtac ggcgcggcca 13920

gcctgctgat gcccaactac gcgctgcatc cttccatcat ccccacgccg ggctaccgcg 13980

gcacgcgctt ctaccgcggt cataccagca gccgccgccg caagaccacc actcgccgcc 14040

gccgtcgccg caccgccgct gcaaccaccc ctgccgccct ggtgcggaga gtgtaccgcc 14100

gcggccgcgc acctctgacc ctgccgcgcg cgcgctacca cccgagcatc gccatttaaa 14160

ctttcgccag ctttgcagat caatggccct cacatgccgc cttcgcgttc ccattacggg 14220

ctaccgagga agaaaaccgc gccgtagaag gctggcgggg aacgggatgc gtcgccacca 14280

ccaccggcgg cggcgcgcca tcagcaagcg gttgggggga ggcttcctgc ccgcgctgat 14340

ccccatcatc gccgcggcga tcggggcgat ccccggcatt gcttccgtgg cggtgcaggc 14400

ctctcagcgc cactgagaca cacttggaaa catcttgtaa taaacccatg gactctgacg 14460

ctcctggtcc tgtgatgtgt tttcgtagac agatggaaga catcaatttt tcgtccctgg 14520

ctccgcgaca cggcacgcgg ccgttcatgg gcacctggag cgacatcggc accagccaac 14580

tgaacggggg cgccttcaat tggagcagtc tctggagcgg gcttaagaat ttcgggtcca 14640

cgcttaaaac ctatggcagc aaggcgtgga acagcaccac agggcaggcg ctgagggata 14700

agctgaaaga gcagaacttc cagcagaagg tggtcgatgg gctcgcctcg ggcatcaacg 14760

gggtggtgga cctggccaac caggccgtgc agcggcagat caacagccgc ctggacccgg 14820

tgccgcccgc cggctccgtg gagatgccgc aggtggagga ggagctgcct cccctggaca 14880

agcggggcga gaagcgaccc cgccccgatg cggaggagac gctgctgacg cacacggacg 14940

agccgccccc gtacgaggag gcggtgaaac tgggtctgcc caccacgcgg cccatcgcgc 15000

ccctggccac cggggtgctg aaacccgaaa agcccgcgac cctggacttg cctcctcccc 15060

agccttcccg cccctctaca gtggctaagc ccctgccgcc ggtggccgtg gcccgcgcgc 15120

gacccggggg caccgcccgc cctcatgcga actggcagag cactctgaac agcatcgtgg 15180

gtctgggagt gcagagtgtg aagcgccgcc gctgctatta aacctaccgt agcgcttaac 15240

ttgcttgtct gtgtgtgtat gtattatgtc gccgccgccg ctgtccacca gaaggaggag 15300

tgaagaggcg cgtcgccgag ttgcaagatg gccaccccat cgatgctgcc ccagtgggcg 15360

tacatgcaca tcgccggaca ggacgcttcg gagtacctga gtccgggtct ggtgcagttt 15420

gcccgcgcca cagacaccta cttcagtctg gggaacaagt ttaggaaccc cacggtggcg 15480

cccacgcacg atgtgaccac cgaccgcagc cagcggctga cgctgcgctt cgtgcccgtg 15540

gaccgcgagg acaacaccta ctcgtacaaa gtgcgctaca cgctggccgt gggcgacaac 15600

cgcgtgctgg acatggccag cacctacttt gacatccgcg gcgtgctgga tcggggccct 15660

agcttcaaac cctactccgg caccgcctac aacagtctgg cccccaaggg agcacccaac 15720

acttgtcagt ggacatataa agccgatggt gaaactgcca cagaaaaaac ctatacatat 15780

ggaaatgcac ccgtgcaggg cattaacatc acaaaagatg gtattcaact tggaactgac 15840

accgatgatc agccaatcta cgcagataaa acctatcagc ctgaacctca agtgggtgat 15900

gctgaatggc atgacatcac tggtactgat gaaaagtatg gaggcagagc tcttaagcct 15960

gataccaaaa tgaagccttg ttatggttct tttgccaagc ctactaataa agaaggaggt 16020

caggcaaatg tgaaaacagg aacaggcact actaaagaat atgacataga catggctttc 16080

tttgacaaca gaagtgcggc tgctgctggc ctagctccag aaattgtttt gtatactgaa 16140

aatgtggatt tggaaactcc agatacccat attgtataca aagcaggcac agatgacagc 16200

agctcttcta ttaatttggg tcagcaagcc atgcccaaca gacctaacta cattggtttc 16260

agagacaact ttatcgggct catgtactac aacagcactg gcaatatggg ggtgctggcc 16320

ggtcaggctt ctcagctgaa tgctgtggtt gacttgcaag acagaaacac cgagctgtcc 16380

taccagctct tgcttgactc tctgggtgac agaacccggt atttcagtat gtggaatcag 16440

gcggtggaca gctatgatcc tgatgtgcgc attattgaaa atcatggtgt ggaggatgaa 16500

cttcccaact attgtttccc tctggatgct gttggcagaa cagatactta tcagggaatt 16560

aaggctaatg gaactgatca aaccacatgg accaaagatg acagtgtcaa tgatgctaat 16620

gagataggca agggtaatcc attcgccatg gaaatcaaca tccaagccaa cctgtggagg 16680

aacttcctct acgccaacgt ggccctgtac ctgcccgact cttacaagta cacgccggcc 16740

aatgttaccc tgcccaccaa caccaacacc tacgattaca tgaacggccg ggtggtggcg 16800

ccctcgctgg tggactccta catcaacatc ggggcgcgct ggtcgctgga tcccatggac 16860

aacgtgaacc ccttcaacca ccaccgcaat gcggggctgc gctaccgctc catgctcctg 16920

ggcaacgggc gctacgtgcc cttccacatc caggtgcccc agaaattttt cgccatcaag 16980

agcctcctgc tcctgcccgg gtcctacacc tacgagtgga acttccgcaa ggacgtcaac 17040

atgatcctgc agagctccct cggcaacgac ctgcgcacgg acggggcctc catctccttc 17100

accagcatca acctctacgc caccttcttc cccatggcgc acaacacggc ctccacgctc 17160

gaggccatgc tgcgcaacga caccaacgac cagtccttca acgactacct ctcggcggcc 17220

aacatgctct accccatccc ggccaacgcc accaacgtgc ccatctccat cccctcgcgc 17280

aactgggccg ccttccgcgg ctggtccttc acgcgtctca agaccaagga gacgccctcg 17340

ctgggctccg ggttcgaccc ctacttcgtc tactcgggct ccatccccta cctcgacggc 17400

accttctacc tcaaccacac cttcaagaag gtctccatca ccttcgactc ctccgtcagc 17460

tggcccggca acgaccggct cctgacgccc aacgagttcg aaatcaagcg caccgtcgac 17520

ggcgagggct acaacgtggc ccagtgcaac atgaccaagg actggttcct ggtccagatg 17580

ctggcccact acaacatcgg ctaccagggc ttctacgtgc ccgagggcta caaggaccgc 17640

atgtactcct tcttccgcaa cttccagccc atgagccgcc aggtggtgga cgaggtcaac 17700

tacaaggact accaggccgt caccctggcc taccagcaca acaactcggg cttcgtcggc 17760

tacctcgcgc ccaccatgcg ccagggccag ccctaccccg ccaactaccc ctacccgctc 17820

atcggcaaga gcgccgtcac cagcgtcacc cagaaaaagt tcctctgcga cagggtcatg 17880

tggcgcatcc ccttctccag caacttcatg tccatgggcg cgctcaccga cctcggccag 17940

aacatgctct atgccaactc cgcccacgcg ctagacatga atttcgaagt cgaccccatg 18000

gatgagtcca cccttctcta tgttgtcttc gaagtcttcg acgtcgtccg agtgcaccag 18060

ccccaccgcg gcgtcatcga ggccgtctac ctgcgcaccc ccttctcggc cggtaacgcc 18120

accacctaag ctcttgcttc ttgcaagcca tggccgcggg ctccggcgag caggagctca 18180

gggccatcat ccgcgacctg ggctgcgggc cctacttcct gggcaccttc gataagcgct 18240

tcccgggatt catggccccg cacaagctgg cctgcgccat cgtcaacacg gccggccgcg 18300

agaccggggg cgagcactgg ctggccttcg cctggaaccc gcgctcgaac acctgctacc 18360

tcttcgaccc cttcgggttc tcggacgagc gcctcaagca gatctaccag ttcgagtacg 18420

agggcctgct gcgccgcagc gccctggcca ccgaggaccg ctgcgtcacc ctggaaaagt 18480

ccacccagac cgtgcagggt ccgcgctcgg ccgcctgcgg gctcttctgc tgcatgttcc 18540

tgcacgcctt cgtgcactgg cccgaccgcc ccatggacaa gaaccccacc atgaacttgc 18600

tgacgggggt gcccaacggc atgctccagt cgccccaggt ggaacccacc ctgcgccgca 18660

accaggaggc gctctaccgc ttcctcaact cccactccgc ctactttcgc tcccaccgcg 18720

cgcgcatcga gaaggccacc gccttcgacc gcatgaatca agacatgtaa accgtgtgtg 18780

tatgttaaat gtctttaata aacagcactt tcatgttaca catgcatctg agatgattta 18840

tttagaaatc gaaagggttc tgccgggtct cggcatggcc cgcgggcagg gacacgttgc 18900

ggaactggta cttggccagc cacttgaact cggggatcag cagtttgggc agcggggtgt 18960

cggggaagga gtcggtccac agcttccgcg tcagttgcag ggcgcccagc aggtcgggcg 19020

cggagatctt gaaatcgcag ttgggacccg cgttctgcgc gcgggagttg cggtacacgg 19080

ggttgcagca ctggaacacc atcagggccg ggtgcttcac gctcgccagc accgtcgcgt 19140

cggtgatgct ctccacgtcg aggtcctcgg cgttggccat cccgaagggg gtcatcttgc 19200

aggtctgcct tcccatggtg ggcacgcacc cgggcttgtg gttgcaatcg cagtgcaggg 19260

ggatcagcat catctgggcc tggtcggcgt tcatccccgg gtacatggcc ttcatgaaag 19320

cctccaattg cctgaacgcc tgctgggcct tggctccctc ggtgaagaag accccgcagg 19380

acttgctaga gaactggttg gtggcgcacc cggcgtcgtg cacgcagcag cgcgcgtcgt 19440

tgttggccag ctgcaccacg ctgcgccccc agcggttctg ggtgatcttg gcccggtcgg 19500

ggttctcctt cagcgcgcgc tgcccgttct cgctcgccac atccatctcg atcatgtgct 19560

ccttctggat catggtggtc ccgtgcaggc accgcagctt gccctcggcc tcggtgcacc 19620

cgtgcagcca cagcgcgcac ccggtgcact cccagttctt gtgggcgatc tgggaatgcg 19680

cgtgcacgaa gccctgcagg aagcggccca tcatggtggt cagggtcttg ttgctagtga 19740

aggtcagcgg aatgccgcgg tgctcctcgt tgatgtacag gtggcagatg cggcggtaca 19800

cctcgccctg ctcgggcatc agctggaagt tggctttcag gtcggtctcc acgcggtagc 19860

ggtccatcag catagtcatg atttccatac ccttctccca ggccgagacg atgggcaggc 19920

tcatagggtt cttcaccatc atcttagcgc tagcagccgc ggccaggggg tcgctctcgt 19980

ccagggtctc aaagctccgc ttgccgtcct tctcggtgat ccgcaccggg gggtagctga 20040

agcccacggc cgccagctcc tcctcggcct gtctttcgtc ctcgctgtcc tggctgacgt 20100

cctgcaggac cacatgcttg gtcttgcggg gtttcttctt gggcggcagc ggcggcggag 20160

atgttggaga tggcgagggg gagcgcgagt tctcgctcac cactactatc tcttcctctt 20220

cttggtccga ggccacgcgg cggtaggtat gtctcttcgg gggcagaggc ggaggcgacg 20280

ggctctcgcc gccgcgactt ggcggatggc tggcagagcc ccttccgcgt tcgggggtgc 20340

gctcccggcg gcgctctgac tgacttcctc cgcggccggc cattgtgttc tcctagggag 20400

gaacaacaag catggagact cagccatcgc caacctcgcc atctgccccc accgccgacg 20460

agaagcagca gcagcagaat gaaagcttaa ccgccccgcc gcccagcccc gccacctccg 20520

acgcggccgt cccagacatg caagagatgg aggaatccat cgagattgac ctgggctatg 20580

tgacgcccgc ggagcacgag gaggagctgg cagtgcgctt ttcacaagaa gagatacacc 20640

aagaacagcc agagcaggaa gcagagaatg agcagagtca ggctgggctc gagcatgacg 20700

gcgactacct ccacctgagc gggggggagg acgcgctcat caagcatctg gcccggcagg 20760

ccaccatcgt caaggatgcg ctgctcgacc gcaccgaggt gcccctcagc gtggaggagc 20820

tcagccgcgc ctacgagttg aacctcttct cgccgcgcgt gccccccaag cgccagccca 20880

atggcacctg cgagcccaac ccgcgcctca acttctaccc ggtcttcgcg gtgcccgagg 20940

ccctggccac ctaccacatc tttttcaaga accaaaagat ccccgtctcc tgccgcgcca 21000

accgcacccg cgccgacgcc cttttcaacc tgggtcccgg cgcccgccta cctgatatcg 21060

cctccttgga agaggttccc aagatcttcg agggtctggg cagcgacgag actcgggccg 21120

cgaacgctct gcaaggagaa ggaggagagc atgagcacca cagcgccctg gtcgagttgg 21180

aaggcgacaa cgcgcggctg gcggtgctca aacgcacggt cgagctgacc catttcgcct 21240

acccggctct gaacctgccc cccaaagtca tgagcgcggt catggaccag gtgctcatca 21300

agcgcgcgtc gcccatctcc gaggacgagg gcatgcaaga ctccgaggag ggcaagcccg 21360

tggtcagcga cgagcagctg gcccggtggc tgggtcctaa tgctagtccc cagagtttgg 21420

aagagcggcg caaactcatg atggccgtgg tcctggtgac cgtggagctg gagtgcctgc 21480

gccgcttctt cgccgacgcg gagaccctgc gcaaggtcga ggagaacctg cactacctct 21540

tcaggcacgg gttcgtgcgc caggcctgca agatctccaa cgtggagctg accaacctgg 21600

tctcctacat gggcatcttg cacgagaacc gcctggggca gaacgtgctg cacaccaccc 21660

tgcgcgggga ggcccggcgc gactacatcc gcgactgcgt ctacctctac ctctgccaca 21720

cctggcagac gggcatgggc gtgtggcagc agtgtctgga ggagcagaac ctgaaagagc 21780

tctgcaagct cctgcagaag aacctcaagg gtctgtggac cgggttcgac gagcgcacca 21840

ccgcctcgga cctggccgac ctcattttcc ccgagcgcct caggctgacg ctgcgcaacg 21900

gcctgcccga ctttatgagc caaagcatgt tgcaaaactt tcgctctttc atcctcgaac 21960

gctccggaat cctgcccgcc acctgctccg cgctgccctc ggacttcgtg ccgctgacct 22020

tccgcgagtg ccccccgccg ctgtggagcc actgctacct gctgcgcctg gccaactacc 22080

tggcctacca ctcggacgtg atcgaggacg tcagcggcga gggcctgctc gagtgccact 22140

gccgctgcaa cctctgcacg ccgcaccgct ccctggcctg caacccccag ctgctgagcg 22200

agacccagat catcggcacc ttcgagttgc aagggcccag cgaaggcgag ggttcagccg 22260

ccaagggggg tctgaaactc accccggggc tgtggacctc ggcctacttg cgcaagttcg 22320

tgcccgagga ctaccatccc ttcgagatca ggttctacga ggaccaatcc catccgccca 22380

aggccgagct gtcggcctgc gtcatcaccc agggggcgat cctggcccaa ttgcaagcca 22440

tccagaaatc ccgccaagaa ttcttgctga aaaagggccg cggggtctac ctcgaccccc 22500

agaccggtga ggagctcaac cccggcttcc cccaggatgc cccgaggaaa caagaagctg 22560

aaagtggagc tgccgcccgt ggaggatttg gaggaagact gggagaacag cagtcaggca 22620

gaggaggagg agatggagga agactgggac agcactcagg cagaggagga cagcctgcaa 22680

gacagtctgg aggaagacga ggaggaggca gaggaggagg tggaagaagc agccgccgcc 22740

agaccgtcgt cctcggcggg ggagaaagca agcagcacgg ataccatctc cgctccgggt 22800

cggggtcccg ctcgaccaca cagtagatgg gacgagaccg gacgattccc gaaccccacc 22860

acccagaccg gtaagaagga gcggcaggga tacaagtcct ggcgggggca caaaaacgcc 22920

atcgtctcct gcttgcaggc ctgcgggggc aacatctcct tcacccggcg ctacctgctc 22980

ttccaccgcg gggtgaactt tccccgcaac atcttgcatt actaccgtca cctccacagc 23040

ccctactact tccaagaaga ggcagcagca gcagaaaaag accagcagaa aaccagcagc 23100

tagaaaatcc acagcggcgg cagcaggtgg actgaggatc gcggcgaacg agccggcgca 23160

aacccgggag ctgaggaacc ggatctttcc caccctctat gccatcttcc agcagagtcg 23220

ggggcaggag caggaactga aagtcaagaa ccgttctctg cgctcgctca cccgcagttg 23280

tctgtatcac aagagcgaag accaacttca gcgcactctc gaggacgccg aggctctctt 23340

caacaagtac tgcgcgctca ctcttaaaga gtagcccgcg cccgcccagt cgcagaaaaa 23400

ggcgggaatt acgtcacctg tgcccttcgc cctagccgcc tccacccatc atcatgagca 23460

aagagattcc cacgccttac atgtggagct accagcccca gatgggcctg gccgccggtg 23520

ccgcccagga ctactccacc cgcatgaatt ggctcagcgc cgggcccgcg atgatctcac 23580

gggtgaatga catccgcgcc caccgaaacc agatactcct agaacagtca gcgctcaccg 23640

ccacgccccg caatcacctc aatccgcgta attggcccgc cgccctggtg taccaggaaa 23700

ttccccagcc cacgaccgta ctacttccgc gagacgccca ggccgaagtc cagctgacta 23760

actcaggtgt ccagctggcg ggcggcgcca ccctgtgtcg tcaccgcccc gctcagggta 23820

taaagcggct ggtgatccgg ggcagaggca cacagctcaa cgacgaggtg gtgagctctt 23880

cgctgggtct gcgacctgac ggagtcttcc aactcgccgg atcggggaga tcttccttca 23940

cgcctcgtca ggccgtcctg actttggaga gttcgtcctc gcagccccgc tcgggtggca 24000

tcggcactct ccagttcgtg gaggagttca ctccctcggt ctacttcaac cccttctccg 24060

gctcccccgg ccactacccg gacgagttca tcccgaactt cgacgccatc agcgagtcgg 24120

tggacggcta cgattgagtt taaactcacc cccttatcca gtgaaataaa gatcatattg 24180

atgatgattt tacagaaata aaaaataatc atttgatttg aaataaagat acaatcatat 24240

tgatgatttg agtttaacaa aaaaataaag aatcacttac ttgaaatctg ataccaggtc 24300

tctgtccatg ttttctgcca acaccacttc actcccctct tcccagctct ggtactgcag 24360

gccccggcgg gctgcaaact tcctccacac gctgaagggg atgtcaaatt cctcctgtcc 24420

ctcaatcttc attttatctt ctatcagatg tccaaaaagc gcgtccgggt ggatgatgac 24480

ttcgaccccg tctaccccta cgatgcagac aacgcaccga ccgtgccctt catcaacccc 24540

cccttcgtct cttcagatgg attccaagag aagcccctgg gggtgttgtc cctgcgactg 24600

gccgaccccg tcaccaccaa gaacggggaa atcaccctca agctgggaga gggggtggac 24660

ctcgattcct cgggaaaact catctccaac acggccacca aggccgccgc ccctctcagt 24720

ttttccaaca acaccatttc ccttaacatg gatcacccct tttacactaa agatggaaaa 24780

ttatccttac aagtttctcc accattaaat atactgagaa caagcattct aaacacacta 24840

gctttaggtt ttggatcagg tttaggactc cgtggctctg ccttggcagt acagttagtc 24900

tctccactta catttgatac tgatggaaac ataaagctta ccttagacag aggtttgcat 24960

gttacaacag gagatgcaat tgaaagcaac ataagctggg ctaaaggttt aaaatttgaa 25020

gatggagcca tagcaaccaa cattggaaat gggttagagt ttggaagcag tagtacagaa 25080

acaggtgttg atgatgctta cccaatccaa gttaaacttg gatctggcct tagctttgac 25140

agtacaggag ccataatggc tggtaacaaa gaagacgata aactcacttt gtggacaaca 25200

cctgatccat caccaaactg tcaaatactc gcagaaaatg atgcaaaact aacactttgc 25260

ttgactaaat gtggtagtca aatactggcc actgtgtcag tcttagttgt aggaagtgga 25320

aacctaaacc ccattactgg caccgtaagc agtgctcagg tgtttctacg ttttgatgca 25380

aacggtgttc ttttaacaga acattctaca ctaaaaaaat actgggggta taggcaggga 25440

gatagcatag atggcactcc atataccaat gctgtaggat tcatgcccaa tttaaaagct 25500

tatccaaagt cacaaagttc tactactaaa aataatatag tagggcaagt atacatgaat 25560

ggagatgttt caaaacctat gcttctcact ataaccctca atggtactga tgacagcaac 25620

agtacatatt caatgtcatt ttcatacacc tggactaatg gaagctatgt tggagcaaca 25680

tttggggcta actcttatac cttctcatac atcgcccaag aatgaacact gtatcccacc 25740

ctgcatgcca acccttccca ccccactctg tggaacaaac tctgaaacac aaaataaaat 25800

aaagttcaag tgttttattg attcaacagt ttcacagaac cctagtattc aacctgccac 25860

ctccctccca acacacagag tacacagtcc tttctccccg gctggcctta aaaagcatca 25920

tatcatgggt aacagacata ttcttaggtg ttatattcca cacggtttcc tgtcgagcca 25980

aacgctcatc agtgatatta ataaactccc cgggcagctc acttaagttc atgtcgctgt 26040

ccagctgctg agccacaggc tgctgtccaa cttgcggttg cttaacgggc ggcgaaggag 26100

aagtccacgc ctacatgggg gtagagtcat aatcgtgcat caggataggg cggtggtgct 26160

gcagcagcgc gcgaataaac tgctgccgcc gccgctccgt cctgcaggaa tacaacatgg 26220

cagtggtctc ctcagcgatg attcgcaccg cccgcagcat aaggcgcctt gtcctccggg 26280

cacagcagcg caccctgatc tcacttaaat cagcacagta actgcagcac agcaccacaa 26340

tattgttcaa aatcccacag tgcaaggcgc tgtatccaaa gctcatggcg gggaccacag 26400

aacccacgtg gccatcatac cacaagcgca ggtagattaa gtggcgaccc ctcataaaca 26460

cgctggacat aaacattacc tcttttggca tgttgtaatt caccacctcc cggtaccata 26520

taaacctctg attaaacatg gcgccatcca ccaccatcct aaaccagctg gccaaaacct 26580

gcccgccggc tatacactgc agggaaccgg gactggaaca atgacagtgg agagcccagg 26640

actcgtaacc atggatcatc atgctcgtca tgatatcaat gttggcacaa cacaggcaca 26700

cgtgcataca cttcctcagg attacaagct cctcccgcgt tagaaccata tcccagggaa 26760

caacccattc ctgaatcagc gtaaatccca cactgcaggg aagacctcgc acgtaactca 26820

cgttgtgcat tgtcaaagtg ttacattcgg gcagcagcgg atgatcctcc agtatggtag 26880

cgcgggtttc tgtctcaaaa ggaggtagac gatccctact gtacggagtg cgccgagaca 26940

accgagatcg tgttggtcgt agtgtcatgc caaatggaac gccggacgta gtcatatttc 27000

ctgaagcaaa accaggtgcg ggcgtgacaa acagatctgc gtctccggtc tcgccgctta 27060

gatcgctctg tgtagtagtt gtagtatatc cactctctca aagcatccag gcgccccctg 27120

gcttcgggtt ctatgtaaac tccttcatgc gccgctgccc tgataacatc caccaccgca 27180

gaataagcca cacccagcca acctacacat tcgttctgcg agtcacacac gggaggagcg 27240

ggaagagctg gaagaaccat gattaacttt attccaaacg gtctcggagc acttcaaaat 27300

gcaggtcccg gaggtggcac ctctcgcccc cactgtgttg gtggaaaata acagccaggt 27360

caaaggtgac acggttctcg agatgttcca cggtggcttc cagcaaagcc tccacgcgca 27420

catccagaaa caagaggaca gcgaaagcgg gagcgttttc taattcctca atcatcatat 27480

tacactcctg caccatcccc agataatttt catttttcca gccttgaatg attcgtatta 27540

gttcctgagg taaatccaag ccagccatga taaaaagctc gcgcagagcg ccctccaccg 27600

gcattcttaa gcacaccctc ataattccaa gagattctgc tcctggttca cctgcagcag 27660

attaacaatg ggaatatcaa aatctctgcc gcgatcccta agctcctccc tcaacaataa 27720

ctgtatgtaa tctttcatat catctccgaa atttttagcc atagggccgc caggaataag 27780

agcagggcaa gccacattac agataaagcg aagtcctccc cagtgwgcat tgccaaatgt 27840

aagattgaaa taagcatgct ggctagaccc tgtgatatct tccagataac tggacagaaa 27900

atcaggcaag caatttttaa gaaaatcaac aaaagaaaag tcgtccaggt gcaggtttag 27960

agcctcagga acaacgatgg aataagtgca aggagtgcgt tccagcatgg ttagtgtttt 28020

tttggtgatc tgtagaacaa aaaataaaca tgcaatatta aaccatgcta gcctggcgaa 28080

caggtgggta aatcactctt tccagcacca ggcaggctac ggggtctccg gcgcgaccct 28140

cgtagaagct gtcgccatga ttgaaaagca tcaccgagag accttcccgg tggccggcat 28200

ggatgattcg agaagaagca tacactccgg gaacattggc atccgtgagt gaaaaaaagc 28260

gacctataaa gcctcggggc actacaatgc tcaatctcaa ttccagcaaa gccaccccat 28320

gcggatggag cacaaaattg gcaggtgcgt aaaaaatgta attactcccc tcctgcacag 28380

gcagcaaagc ccccgctccc tccagaaaca catacaaagc ctcagcgtcc atagcttacc 28440

gagcacggca ggcgcaagag tcagagaaaa ggctgagctc taacctgact gcccgctcct 28500

gtgctcaata tatagcccta acctacactg acgtaaaggc caaagtctaa aaatacccgc 28560

caaataatca cacacgccca gcacacgccc agaaaccggt gacacactca aaaaaatacg 28620

cgcacttcct caaacgccca aaactgccgt catttccggg ttcccacgct acgtcatcaa 28680

aacacgactt tcaaattccg tcgaccgtta aaaacgtcac ccgccccgcc cctaacggtc 28740

gcccgtctct cagccaatca gcgccccgca tccccaaatt caaacacctc atttgcatat 28800

taacgcgcac aaaaagtttg aggtatatta ttgatgatgg ttaattaagg atccttctat 28860

agtgtcacct aaatgtcgac ggccaggcgg ccgccaggcc tacccactag tcaattcggg 28920

aggatcgaaa cggcagatcg caaaaaacag tacatacaga aggagacatg aacatgaaca 28980

tcaaaaaaat tgtaaaacaa gccacagttc tgacttttac gactgcactt ctggcaggag 29040

gagcgactca agccttcgcg aaagaaaata accaaaaagc atacaaagaa acgtacggcg 29100

tctctcatat tacacgccat gatatgctgc agatccctaa acagcagcaa aacgaaaaat 29160

accaagtgcc tcaattcgat caatcaacga ttaaaaatat tgagtctgca aaaggacttg 29220

atgtgtggga cagctggccg ctgcaaaacg ctgacggaac agtagctgaa tacaacggct 29280

atcacgttgt gtttgctctt gcgggaagcc cgaaagacgc tgatgacaca tcaatctaca 29340

tgttttatca aaaggtcggc gacaactcaa tcgacagctg gaaaaacgcg ggccgtgtct 29400

ttaaagacag cgataagttc gacgccaacg atccgatcct gaaagatcag acgcaagaat 29460

ggtccggttc tgcaaccttt acatctgacg gaaaaatccg tttattctac actgactatt 29520

ccggtaaaca ttacggcaaa caaagcctga caacagcgca ggtaaatgtg tcaaaatctg 29580

atgacacact caaaatcaac ggagtggaag atcacaaaac gatttttgac ggagacggaa 29640

aaacatatca gaacgttcag cagtttatcg atgaaggcaa ttatacatcc ggcgacaacc 29700

atacgctgag agaccctcac tacgttgaag acaaaggcca taaatacctt gtattcgaag 29760

ccaacacggg aacagaaaac ggataccaag gcgaagaatc tttatttaac aaagcgtact 29820

acggcggcgg cacgaacttc ttccgtaaag aaagccagaa gcttcagcag agcgctaaaa 29880

aacgcgatgc tgagttagcg aacggcgccc tcggtatcat agagttaaat aatgattaca 29940

cattgaaaaa agtaatgaag ccgctgatca cttcaaacac ggtaactgat gaaatcgagc 30000

gcgcgaatgt tttcaaaatg aacggcaaat ggtacttgtt cactgattca cgcggttcaa 30060

aaatgacgat cgatggtatt aactcaaacg atatttacat gcttggttat gtatcaaact 30120

ctttaaccgg cccttacaag ccgctgaaca aaacagggct tgtgctgcaa atgggtcttg 30180

atccaaacga tgtgacattc acttactctc acttcgcagt gccgcaagcc aaaggcaaca 30240

atgtggttat cacaagctac atgacaaaca gaggcttctt cgaggataaa aaggcaacat 30300

ttgcgccaag cttcttaatg aacatcaaag gcaataaaac atccgttgtc aaaaacagca 30360

tcctggagca aggacagctg acagtcaact aataacagca aaaagaaaat gccgatactt 30420

cattggcatt ttcttttatt tctcaacaag atggtgaatt gactagtggg tagatccaca 30480

ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca 30540

ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt 30600

gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga 30660

cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca 30720

gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac 30780

tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc 30840

aaacatgaga attgatccgg aacccttaat ataacttcgt ataatgtatg ctatacgaag 30900

ttattaggtc cctcgactat agggtcaccg tcgacagcga cacacttgca tcggatgcag 30960

cccggttaac gtgccggcac ggcctgggta accaggtatt ttgtccacat aaccgtgcgc 31020

aaaatgttgt ggataagcag gacacagcag caatccacag caggcataca accgcacacc 31080

gaggttactc cgttctacag gttacgacga catgtcaata cttgcccttg acaggcattg 31140

atggaatcgt agtctcacgc tgatagtctg atcgacaata caagtgggac cgtggtccca 31200

gaccgataat cagaccgaca acacgagtgg gatcgtggtc ccagactaat aatcagaccg 31260

acgatacgag tgggaccgtg gtcccagact aataatcaga ccgacgatac gagtgggacc 31320

gtggttccag actaataatc agaccgacga tacgagtggg accgtggtcc cagactaata 31380

atcagaccga cgatacgagt gggaccatgg tcccagacta ataatcagac cgacgatacg 31440

agtgggaccg tggtcccagt ctgattatca gaccgacgat acgagtggga ccgtggtccc 31500

agactaataa tcagaccgac gatacgagtg ggaccgtggt cccagactaa taatcagacc 31560

gacgatacga gtgggaccgt ggtcccagtc tgattatcag accgacgata caagtggaac 31620

agtgggccca gagagaatat tcaggccagt tatgctttct ggcctgtaac aaaggacatt 31680

aagtaaagac agataaacgt agactaaaac gtggtcgcat cagggtgctg gcttttcaag 31740

ttccttaaga atggcctcaa ttttctctat acactcagtt ggaacacgag acctgtccag 31800

gttaagcacc attttatcgc ccttatacaa tactgtcgct ccaggagcaa actgatgtcg 31860

tgagcttaaa ctagttcttg atgcagatga cgttttaagc acagaagtta aaagagtgat 31920

aacttcttca gcttcaaata tcaccccagc ttttttctgc tcatgaaggt tagatgcctg 31980

ctgcttaagt aattcctctt tatctgtaaa ggctttttga agtgcatcac ctgaccgggc 32040

agatagttca ccggggtgag aaaaaagagc aacaactgat ttaggcaatt tggcggtgtt 32100

gatacagcgg gtaataatct tacgtgaaat attttccgca tcagccagcg cagaaatatt 32160

tccagcaaat tcattctgca atcggcttgc ataacgctga ccacgttcat aagcacttgt 32220

tgggcgataa tcgttaccca atctggataa tgcagccatc tgctcatcat ccagctcgcc 32280

aaccagaaca cgataatcac tttcggtaag tgcagcagct ttacgacggc gactcccatc 32340

ggcaatttct atgacaccag atactcttcg accgaacgcc ggtgtctgtt gaccagtcag 32400

tagaaaagaa gggatgagat catccagtgc gtcctcagta agcagctcct ggtcacgttc 32460

attacctgac catacccgag aggtcttctc aacactatca ccccggagca cttcaagagt 32520

aaacttcaca tcccgaccac atacaggcaa agtaatggca ttaccgcgag ccattactcc 32580

tacgcgcgca attaacgaat ccaccatcgg ggcagctggt gtcgataacg aagtatcttc 32640

aaccggttga gtattgagcg tatgttttgg aataacaggc gcacgcttca ttatctaatc 32700

tcccagcgtg gtttaatcag acgatcgaaa atttcattgc agacaggttc ccaaatagaa 32760

agagcatttc tccaggcacc agttgaagag cgttgatcaa tggcctgttc aaaaacagtt 32820

ctcatccgga tctgaccttt accaacttca tccgtttcac gtacaacatt ttttagaacc 32880

atgcttcccc aggcatcccg aatttgctcc tccatccacg gggactgaga gccattacta 32940

ttgctgtatt tggtaagcaa aatacgtaca tcaggctcga accctttaag atcaacgttc 33000

ttgagcagat cacgaagcat atcgaaaaac tgcagtgcgg aggtgtagtc aaacaactca 33060

gcaggcgtgg gaacaatcag cacatcagca gcacatacga cattaatcgt gccgataccc 33120

aggttaggcg cgctgtcaat aactatgaca tcatagtcat gagcaacagt ttcaatggcc 33180

agtcggagca tcaggtgtgg atcggtgggc agtttacctt catcaaattt gcccattaac 33240

tcagtttcaa tacggtgcag agccagacag gaaggaataa tgtcaagccc cggccagcaa 33300

gtgggcttta ttgcataagt gacatcgtcc ttttccccaa gatagaaagg caggagagtg 33360

tcttctgcat gaatatgaag atctggtacc catccgtgat acattgaggc tgttccctgg 33420

gggtcgttac cttccacgag caaaacacgt agccccttca gagccagatc ctgagcaaga 33480

tgaacagaaa ctgaggtttt gtaaacgcca cctttatggg cagcaacccc gatcaccggt 33540

ggaaatacgt cttcagcacg tcgcaatcgc gtaccaaaca catcacgcat atgattaatt 33600

tgttcaattg tataaccaac acgttgctca acccgtcctc gaatttccat atccgggtgc 33660

ggtagtcgcc ctgctttctc ggcatctctg atagcctgag aagaaacccc aactaaatcc 33720

gctgcttcac ctattctcca gcgccgggtt attttcctcg cttccgggct gtcatcatta 33780

aactgtgcaa tggcgatagc cttcgtcatt tcatgaccag cgtttatgca ctggttaagt 33840

gtttccatga gtttcattct gaacatcctt taatcattgc tttgcgtttt tttattaaat 33900

cttgcaattt actgcaaagc aacaacaaaa tcgcaaagtc atcaaaaaac cgcaaagttg 33960

tttaaaataa gagcaacact acaaaaggag ataagaagag cacatacctc agtcacttat 34020

tatcactagc gctcgccgca gccgtgtaac cgagcatagc gagcgaactg gcgaggaagc 34080

aaagaagaac tgttctgtca gatagctctt acgctcagcg caagaagaaa tatccaccgt 34140

gggaaaaact ccaggtagag gtacacacgc ggatagccaa ttcagagtaa taaactgtga 34200

taatcaaccc tcatcaatga tgacgaacta acccccgata tcaggtcaca tgacgaaggg 34260

aaagagaagg aaatcaactg tgacaaactg ccctcaaatt tggcttcctt aaaaattaca 34320

gttcaaaaag tatgagaaaa tccatgcagg ctgaaggaaa cagcaaaact gtgacaaatt 34380

accctcagta ggtcagaaca aatgtgacga accaccctca aatctgtgac agataaccct 34440

cagactatcc tgtcgtcatg gaagtgatat cgcggaagga aaatacgata tgagtcgtct 34500

ggcggccttt ctttttctca atgtatgaga ggcgcattgg agttctgctg ttgatctcat 34560

taacacagac ctgcaggaag cggcggcgga agtcaggcat acgctggtaa ctttgaggca 34620

gctggtaacg ctctatgatc cagtcgattt tcagagagac gatgcctgag ccatccggct 34680

tacgatactg acacagggat tcgtataaac gcatggcata cggattggtg atttcttttg 34740

tttcactaag ccgaaactgc gtaaaccggt tctgtaaccc gataaagaag ggaatgagat 34800

atgggttgat atgtacactg taaagccctc tggatggact gtgcgcacgt ttgataaacc 34860

aaggaaaaga ttcatagcct ttttcatcgc cggcatcctc ttcagggcga taaaaaacca 34920

cttccttccc cgcgaaactc ttcaatgcct gccgtatatc cttactggct tccgcagagg 34980

tcaatccgaa tatttcagca tatttagcaa catggatctc gcagataccg tcatgttcct 35040

gtagggtgcc atcagatttt ctgatctggt caacgaacag atacagcata cgtttttgat 35100

cccgggagag actatatgcc gcctcagtga ggtcgtttga ctggacgatt cgcgggctat 35160

ttttacgttt cttgtgattg ataaccgctg tttccgccat gacagatcca tgtgaagtgt 35220

gacaagtttt tagattgtca cactaaataa aaaagagtca ataagcaggg ataactttgt 35280

gaaaaaacag cttcttctga gggcaatttg tcacagggtt aagggcaatt tgtcacagac 35340

aggactgtca tttgagggtg atttgtcaca ctgaaagggc aatttgtcac aacaccttct 35400

ctagaaccag catggataaa ggcctacaag gcgctctaaa aaagaagatc taaaaactat 35460

aaaaaaaata attataaaaa tatccccgtg gataagtgga taaccccaag ggaagttttt 35520

tcaggcatcg tgtgtaagca gaatatataa gtgctgttcc ctggtgcttc ctcgctcact 35580

cgagggcttc gccctgtcgc tcaactgcgg cgagcactac tggctgtaaa aggacagacc 35640

acatcatggt tctgtgttca ttaggttgtt ctgtccattg ctgacataat ccgctccact 35700

tcaacgtaac accgcacgaa gatttctatt gttcctgaag gcatattcaa atcgttttcg 35760

ttaccgcttg caggcatcat gacagaacac tacttcctat aaacgctaca caggctcctg 35820

agattaataa tgcggatctc tacgataatg ggagattttc ccgactgttt cgttcgcttc 35880

tcagtggata acagccagct tctctgttta acagacaaaa acagcatatc cactcagttc 35940

cacatttcca tataaaggcc aaggcattta ttctcaggat aattgtttca gcatcgcaac 36000

cgcatcagac tccggcatcg caaactgcac ccggtgccgg gcagccacat ccagcgcaaa 36060

aaccttcgtg tagacttccg ttgaactgat ggacttatgt cccatcaggc tttgcagaac 36120

tttcagcggt ataccggcat acagcatgtg catcgcatag gaatggcgga acgtatgtgg 36180

tgtgaccgga acagagaacg tcacaccgtc agcagcagcg gcggcaaccg cctccccaat 36240

ccaggtcctg accgttctgt ccgtcacttc ccagatccgc gctttctctg tccttcctgt 36300

gcgacggtta cgccgctcca tgagcttatc gcgaataaat acctgtgacg gaagatcact 36360

tcgcagaata aataaatcct ggtgtccctg ttgataccgg gaagccctgg gccaactttt 36420

ggcgaaaatg agacgttgat cggcacgtaa gaggttccaa ctttcaccat aatgaaataa 36480

gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa 36540

tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac 36600

attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata 36660

ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc 36720

acattcttgc ccgcctgatg aatgctcatc cggagttccg tatggcaatg aaagacggtg 36780

agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa 36840

cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt 36900

cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga 36960

atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg 37020

ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg 37080

acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg 37140

tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat 37200

ttttttaagg cagttattgg tgcccttaaa cgcctggttg ctacgcctga ataagtgata 37260

ataagcggat gaatggcaga aattcgatga taagctgtca aacatgagaa ttggtcgacg 37320

gcgcgccaaa gcttgcatgc ctgcagccgc gtaacctggc aaaatcggtt acggttgagt 37380

aataaatgga tgccctgcgt aagcggggca catttcatta cctctttctc cgcacccgac 37440

atagataata acttcgtata gtatacatta tacgaagtta tctagtagac 37490

<210> 69

<211> 143055

<212> DNA

<213> 人工序列

<220>

<223> 病毒构建体

<400> 69

tatttattta gtgtctagaa aaaaatgtgt gaccaacgac cgtaggaaac tctagagggt 60

aagaaaaatc aatcgcttta tagagaccat cagaaagagg tttaatattt ttgtgagacc 120

atcgaaggag aaagagataa aactttttta cgactccatc agaaagaggt ttaatatttt 180

tgtgagacca tcgaagagag aaagagataa aactttttta cgactccatc agaaagaggt 240

ttaatatttt tgtgagacca tcgaagagag aaagagataa aactttttta cgactccatc 300

agaaagaggt ttaatatttt tgtgagacca tcgaaggaga aagagataaa acttttttac 360

gactccatca gaaagaggtt taatattttt gtgagaccat cgaaggagaa agagataaaa 420

cttttttacg actccatcag aaagaggttt aatatttttg tgagaccatc gaaggagaaa 480

gagataaaac ttttttacga ctccatcaga aagaggttta atatttttgt gagaccatcg 540

aagagagaaa gagataaaac ttttttacga ctccatcaga aagaggttta atatttttgt 600

gagaccatcg aaggagaaag agataaaact tttttacgac tccatcagaa agaggtttaa 660

tatttttgtg agaccatcga agagagaaag agaataaaaa tattttagtg acaccatcag 720

aaagaggttt aatatttttg tgagaccatc gaagagagaa agagataaaa cttttttacg 780

actccatcag aaagaggttt aatatttttg tgagaccatc gaaggagaaa gagataaaac 840

ttttttacga ctccatcaga aagaggttta atatttttgt gagaccatcg aagagagaaa 900

gagataaaac ttttttacga ctccatcaga aagaggttta atatttttgt gagaccatcg 960

aagagagaaa gagataaaac ttttttacga ctccatcaga aagaccatcg aagagagaaa 1020

gagaaagaga tagttagtct agatattttt cttagtacaa aagtcaatgt tttaaaatat 1080

atggacaaga atttgtctgt ataaaaactt gtgtgaaatt ttgtaccaaa gaaaaaatgt 1140

gagcagtatc ccctacatgg attttactag atcatttata taccaaaaaa tattatacga 1200

tctacgtttt attatatgat tttaacgtgt aaattataaa cattatttta tgatatacaa 1260

ttgtctggta acctagatgg gcatagggga tgagtatatg ttgttggacg ttattgttta 1320

agaaatagtt gatgcatcag aaagaggttt aatatttttg tgagaccatc gaagagagaa 1380

agagataaaa cttttttacg actccatcag aaagaggttt aatatttttg tgagaccatc 1440

gaagagagaa agagataaaa cttttttatg actccattga agagagaatg agaataaaaa 1500

tattttagtg acaccatcag aaagaggttt aatatttttt atgagaccat caaagagaga 1560

aagagaataa aaatatttta tgactccatt gaagagagaa agagaaaatg agaataaaaa 1620

tattttagtg acaccatcag aaagaggttt aatatttttt atgagaccat caaagagaga 1680

aagagaataa aaatattttt gtaaaacttt ttttatgaga ccatcaaaga gagaaagaga 1740

ataaaaatat ttttgtaaaa ctttttttat gagaccatca aagagagaaa gagaataaaa 1800

atatttttgt aaaacttttt ttatgagacc atcaaagaga gaaagagaat aaaaatattt 1860

ttgtaaaact ttttttatga gaccatcaaa gagagaaaga gaataaaaat atttttgtaa 1920

aacttttttt atgagaccat caaagagaga aagagaataa aaatattttt gtaaaacttt 1980

ttttatgaga ccatcaaaga gagaaagaga ataaaaatat ttttgtaaaa ctttttttat 2040

gagaccatca aagagagaaa gagaataaaa atattttatg actccattga agagagaaag 2100

agaataaaaa tattttagtg acaccatcag aaagaggttt aatatttttg tgagaccatc 2160

gaagagagaa agagaataaa aatattttat gactccattg aagagagaaa gagaataaaa 2220

atattttagt gacaccatca gaaagaggtt taatattttt tatgagacca tcaaagagag 2280

aaagagaata aaaatatttt tgtaaaactt tttttatgag accatcaaag agagaaagag 2340

aataaaaata tttttgtaaa acttttttta tgagaccatc aaagagagaa agagaataaa 2400

aatatttttg taaaactttt tttatgagac catcaaagag agaaagagaa taaaaatatt 2460

tttgtaaaac tttttttatg agaccatcaa agagagaaag agaataaaaa tatttttgta 2520

aaactttttt tatgagacca tcaaagagag aaagagaata aaaatatttt atgactccat 2580

tgaagagaga atgagaataa aaatatttta gtgacaccat cagaaagagg tttaatattt 2640

ttgtgagacc atcgaagaga gaaagagaat aaaaatattt tatgactcca ttgaagagag 2700

aaagagaata aaaatatttt agtgacacca tcagaaagag gtttaatatt ttttatgaga 2760

ccatcaaaga gagaaagaga ataaaaatat ttttgtaaaa ctttttttat gagaccatca 2820

aagagagaaa gagaataaaa atatttttgt aaaacttttt ttatgagacc atcaaagaga 2880

gaaagagaat aaaaatattt ttgtaaaact ttttttatga gaccatcaaa gagagaaaga 2940

gaataaaaat atttttgtaa aacttttttt atgagaccat caaagagaga aagagaataa 3000

aaatattttt gtaaaacttt ttttatgaga ccatcaaaga gagaaagaga ataaaaatat 3060

ttttgtaaaa ctttttttat gagaccatca aagagagaaa gagaataaaa atatttttgt 3120

aaaacttttt ttatgagacc atcaaagaga gaaagagaat aaaaatattt tatgactcca 3180

ttgaagagag aatgagaata aaaatatttt agtgacacca tcagaaagag gtttaatatt 3240

tttgtgagac catcgaagag agaaagagaa taaaaatatt ttatgactcc attgaagaga 3300

gaatgagaat aaaaatattt tagtgacacc atcagaaaga ggtttaatat tttttatgag 3360

accatcaaag agagaaagag aataaaaata tttttgtaaa acttttttta tgagaccatc 3420

aaagagagaa agagaataaa aatatttttg taaaaattat aaacattatt ttatgatata 3480

caattgtctg gtaacctaga tgggcatagg ggatgttgat aagctcgacg agtatatgtt 3540

gttggacgtt attgtttaag aaatagttga tgcatcagaa agagaataaa aaatatttta 3600

gtgagaccat cgaagagaga aagagataaa acttttttac gactccatca gaaagaggtt 3660

taatattttt gtgagaccat cgaagagaga aagagataaa acttttttac gactccatca 3720

gaaagaggtt taatattttt gtgagaccat cgaaggagaa agagataaaa cttttttacg 3780

actccatcag aaagaggttt aatatttttg tgagaccatc aaagagagaa agagaataaa 3840

aatatttttg taaaactttt tttatgagac catcaaagag agaaagagaa taaaaatatt 3900

tttgtaaaac tttttttatg agaccatcaa agagagaaag agaataaaaa tatttttgta 3960

aaactttttt tatgagacca tcaaagagag aaagagaata aaaatatttt tgtaaaactt 4020

tttttatgag accatcaaag agagaaagag aataaaaata ttttatgact ccattgaaga 4080

gagaatgaga ataaaaatat tttagtgaca ccatcagaaa gaggtttaat atttttgtga 4140

gaccatcgaa gagagaaaga gaataaaaat attttatgac tccattgaag agagaatgag 4200

aataaaaata ttttagtgac accatcagaa agaggtttaa tattttttat gagaccatca 4260

aagagagaaa gagaataaaa atatttttgt aaaacttttt ttatgagacc atcaaagaga 4320

gaaagagaat aaaaatattt ttgtaaaact ttttttatga gaccatcaaa gagagaaaga 4380

gaataaaaat atttttgtaa aacttttttt atgagaccat caaagagaga aagagaataa 4440

aaatattttt gtaaaacttt ttttatgaga ccatcaaaga gagaaagaga ataaaaatat 4500

tttatgactc cattgaagag agaatgagaa taaaaatatt ttagtgacac catcagaaag 4560

aggtttaata tttttgtgag accatcgaag agagaaagag aataaaaata ttttatgact 4620

ccattgaaga gagaaagaga ataaaaatat tttagtgaca ccatcagaaa gaggtttaat 4680

attttttatg agaccatcaa agagagaaag agaataaaaa tatttttgta aaactttttt 4740

tatgagacca tcaaagagag aaagagaata aaaatatttt atgactccat tgaagagaga 4800

atgagaataa aaatatttta gtgacaccat cagaaagagg tttaatattt ttgtgagacc 4860

atcgaagaga gaaagagaat aaaaatattt tatgactcca ttgaagagag aatgagaata 4920

aaaatatttt agtgacacca tcagaaagag gtttaatatt tttgtgagac catcgaagag 4980

agaaagagaa taaaaatatt ttatgactcc attgaagaga gaatgagaat aaaaatattt 5040

tagtgacacc atcagaaaga ggtttaatat tttttatgag accatcaaag agagaaagag 5100

aataaaaata tttttgtaaa acttttttta tgagaccatc aaagagagaa agagaataaa 5160

aatatttttg taaaactttt tttatgagac catcaaagag agaaagagaa taaaaatatt 5220

tttgtaaaac tttttttatg agaccatcaa agagagaaag agaataaaaa tatttttgtg 5280

agaccatcaa agagagaaag agaataaaaa tatttttgtg agaccatcaa agagagaaag 5340

agaataaaaa tatttttgtg agaccatcaa agagagaaag agaataaaaa tattttatga 5400

ctccattgaa gagagaaaga gaataaaaat attttagtga caccatcaga aagaggttta 5460

atatttttgt gagaccatcg aagagagaaa gagaataaaa atattttatg actccattga 5520

agagagaaag agaataaaaa tattttagtg acaccatcag aaagaggttt aatatttttt 5580

atgagaccat caaagagaga aagagaataa aaatattttt tatgagacca tcaaagagag 5640

aaagagaata aaaatatttt atgagaccat caaagagaga aagagaataa aaatattttt 5700

tatgagacca tcaaagagag aaagagaata aaaatatttt atgagaccat caaagagaga 5760

aagagaataa aaatattttt tatgagacca tcaaagagag aaagagaata aaaatatttt 5820

tgtatgagac catcagaaag aggtttaata tttttgtgat accctgaaag gaaataggaa 5880

tagtgtcata atcgtatcac actattgaga cagaaaaaga agaagtcgcg agaggtaact 5940

ttttgttttg caaaccggaa tatagtgtcc ggtacacttt tttaattcgt ggtgtgcctg 6000

aatcgttcga ttaaccctac tcatccaatt tcagatgaat agagttatcg attcagacac 6060

acgctttgag ttttgttgaa tcgatgagtg aagtatcatc ggttgcacct tcagatgccg 6120

atccgtcgac atacttgacc tcaagttcag atgattcctt gcacatgtct ccgatacgaa 6180

cgctaaactc tagattcttg acacattttg tatcgacgat cgttgaaccg atgatatctt 6240

cgtaactcac tttcttatga gagatgttag acccgagtac tggatgggtc ttgatgtcgc 6300

tgtctttctc ttcttcgcta catctgatgt cgatagacac ctcacagtct ttccatcagc 6360

ggattctgag atggatttaa tctgaggaca tttggtgaat ccaaagttca ttctcagacc 6420

tccaccgatg atggagtaat aagtggtagg aggatctaca tcctcgactg attccacctc 6480

gggatctgga tctgactcgg actctgtaat ttccgttacg gattggcaaa tcttatcatc 6540

ggtcggtgtt tggtcttgct ttgtgacttt gataataaca tcgattccca tatgatgttt 6600

gttttcttct tccgtacacg atgaggatga ttgctgaaga ctggcaggca catgcatgcc 6660

agtacgatat attgtttcat gattgctatt gattgagtac tgttctttat gattctactt 6720

ccttaccgtg caataaatta gaatatattt tctactttta cgagaaatta attattgtat 6780

ttatgggtga aaaacttact ataaaaagcg ggtgggtttg gaattagtga tcagtttatg 6840

tatatcgcaa ctaccgggca tatggctaca ttacccacat gataagagat tgtatcagtt 6900

tcgtagtctt gagtattggt attactatat agtatataga tgtcgacgct agagttactg 6960

tctccgaatg cggcatgata gtatcattct ttgctttcgt taactgtttg gaggaagaat 7020

ctttgttatt gcatttaatc tcgaaattca gagtgcacac ctttctcctg taaagaaacc 7080

tgaagtcgct accttattaa gaagacggga tcgcagtctt tatgattcat agtaatagtt 7140

agttccgacg ttgagatgga ttcgctgaga ccggtagtgg tcgtccgagt acacgatgtg 7200

tcgttaactg gatacaggtt aatttccaca tcgatatagt taaaggtatt tctgggtacg 7260

ggttcgcatt tatctgcgga agagacggtg tgagaatatg ttccgagacc acacggagaa 7320

cagatgacgt ctccggatac tccgtatcct attccacatt ttgtttggga aacacatgcc 7380

ttgcatccat gatcgggaga gcattcacag attctattgt gagtcgtgtt acacgatcgc 7440

gtcgacattg ttgacagaaa cgtgaccttc attcttaccg tcgtccataa atacgttagg 7500

tatgtaccac atactgtcgc gaacgatgcg tccatctcat aatgatttac tttttcataa 7560

ttaaagatgt gaaagaaaac cgaacaatat atttttttag taatgtttat gcgagacata 7620

taaaataaac tccgtgttta tgatgccggt aaatgttttt atcatcttgg acggaatcga 7680

ttttgtaata tgccatggaa acaggacatt atcactccat gataaattat ttaatggagt 7740

cgatcctctc attgttcttt gcgtatctca atctgtggcg tttgcttcgt ttaaataata 7800

tatcaaacat ggagacgcct gatatgtagg cattcttcat tctattaatg tctgctctat 7860

agcgctttag ttccttatga cgaccggcga tatcatactt actttagaag gaaaatcatc 7920

atctaggatt aaggcgtatc tgatacaggc gaataatggt tcaggatata gatagcgtat 7980

atctctatta aatgcgtcaa tcatagtctc tagagtggga tggtaactca gtaataaatc 8040

aactagcttc tctttggtaa ctgcttttct ggatggccgt attgattatc gagcgtgaca 8100

ctcgctccat attccaataa ccgctttgca aattgtatat tattgacatc gaccgcgtaa 8160

tatagtagag ttatcgatca tatctatatc atccatgtac ttgcttagta tatcaaatac 8220

atcttcataa cagtgatacc cgcaattatt aaatctcgat aatatcagac cgtacataca 8280

tagacggcca ttgttagata tgtgatttac agccgcgtgt ccatattttc cacgataaac 8340

cttacgacgt ttacatcgac gagattatta ttaacaaagt tgttgtccgt cgtcttatcc 8400

aacatgcatt gaatgatagg tatacttacc atatcgccgt aatgtaagta gtttatcagt 8460

atggcttgta cgatggattc atcctgttgt ctaaatctct ttagaatgtt atcgatgatg 8520

tagtggttat attctctgga atcgtacgaa gtaatactac gcattacgtc gacaagagta 8580

tgacgtctct caataagaag attaacgatt tccatgtcta cattatatgg ggttactcta 8640

aatcgcttgt ttagataata cgcctctaat atagggctga cgtcgtatac tctacacgtg 8700

tccacatcct ttattaataa tctctatatc tatggttgag caagaccagt agtattggat 8760

ggaaacattg ttatcgatca aacatttaat tacatccttg gatagagatt ctctatgaga 8820

cgatatatag taatgaagag agttcttaca catatcactg ttgtacatac aggtacgaaa 8880

tacgtaaccg gtgctgtaac attctgattt aagaagccat agcaatactt ctggtctcgg 8940

attaggcgtc gttacgtata tatccaccaa tccgagacca ttgattgcat aattcgtatt 9000

cttggacgga cgtatccgtt tatccacaat taggtatttt agcagacgta agtcgaaatc 9060

atttatattc gacttgagtt cgttagagga attcgaatag ctggatatca gtagatgcac 9120

aatctgagat tttacgtatc tatgcttact gtatgctcct agcggagtta atccttcgtt 9180

gtttctacaa agtctctcga ctccgcgaga gagtaacagt cgaacaatct taatgtctgt 9240

atcgcattta ttggagacgt aacaatgtag cgcattgttt cctcgtctat ctatatgttt 9300

tgataagttg tgacacgttt caatttctag ttttattttt ttgtacgtca catcttcatc 9360

cagtagacga catagaatac atgtgcaatc catagctatt ctggtgctaa ttattcctca 9420

taagatgata aaaagtgtag tgagagagca tgaaggagat ttagtattta gcagtgcgga 9480

tatgatccaa gagggtgaga tagtcgttct cgttcagaat ctttcgcagc ataagtagta 9540

tgtcgatata cttatcgttg aagactcttc cagagacgat agctgattga gtacaaagtc 9600

caatgattgc acgaagttct tcggcggttt tcatggagtc atttaatgat ctccacggaa 9660

gtgaatcctt caactcacca ccaaagagct ccgttgcatc agttctgaaa gagatgagaa 9720

gcctgtagag agaccctgcg ctttctctat gggtccatct atgagaaacc cacaggatgt 9780

attcagtcag acaatgtctg acgtcggcca cggtattcag ggagtcctta gtagcgtggc 9840

aatgacaggg tctgaactgg gcacaaggaa aggccattgt aaaggtagac ctgtagccgt 9900

ttatgctaat agagggcttt aatttccatt ttttaatggg ttgtggatga ggaatgagag 9960

tgatatcata ttgagatacg tagttatgta gaggtgtatt tcctatatta tttactttcg 10020

gtttcatatt ttaccaactc tttaataaat ttcttttcac gatgcatctt attaaatgac 10080

gttttctcat aagtggacat atagatgcag aagtattacc tctatcatct acataattag 10140

ggtctgctcc taacttatac agtacgtagt agtagtttat cggttttaaa tcaagtctag 10200

aatatatagt ggattaatat atttttatat tagctaaagc atatcattct caacttcatc 10260

atgagttaaa tatttgtgtc tactagtttg tttatatcac agcattctac aaacagtcta 10320

aacaatagag aagacggaca gactttaacg tataaatgac acatgttatc gatattcgtt 10380

gataaatgat tctaacgaca tctctcgcta gagataaaat ctagtatcgt atcatactcg 10440

catagcatag tttttcataa ttaatacaat atttaaaaga cttattcgga aagtatttta 10500

atacatgtat catcgatgga gatccatatg aggagtcact tgtagttctt cagtagtgct 10560

atcatcgata gtataattat atgttgttgt aattggagta actgttggta gttcttccgt 10620

ggaatcaata attatactaa cagcaatagt ataattatat aaatatgttc cgttgatatc 10680

acatatttta atgaactcat ttctaaagcc gtacatccac atctattagg atctgatatt 10740

ttacacaact gtttaataga gtctacattt atatgttctc tatcggtgag atacaaatac 10800

ctagatagtc gcgttatagc acaaatacga tataaataat aataataatt attcagtaat 10860

gtatataaaa atgcattgtg tatttactcc aatactactg tagttgtaag ggttttttca 10920

aaataatagt tgtgtccacg acatttatat gtattaccta tatattcttc agtaacattt 10980

tcaaagtaca aggtcgcctc ggtgataccg cctctactag ttaaaacaga gtatacatca 11040

aaatcgaatc ctataagcca tccggatgga ttttcccatt caatcagtac atcgtcaatc 11100

aataatgacg ttgacacagc agtgcatgtt atattggcag gttctcctat cgttacgttg 11160

atttttggat ctagtattag tttaaacctg tggtcttgcg acggtataac cgtaagtatt 11220

ttacatcttg attctaacgt cgtcgtaatg aacgtaacag ttgtatcttc cactatcttc 11280

taactctgga ttatgaataa ttaatttctt tcccgtttgt gaatacttaa tatcgtcgat 11340

attaatttcc ttattatctt tataccaagt tatattatta taatgttttg cgtaaagaat 11400

tccacagtat aagtctatgc catacttatc atgagtacct agttcatatg tttttggaat 11460

gcatgaagga ggttttttaa tatgagatct aactataccc tgaacacagt caccattctt 11520

tgtagttacg gtacacaaat acctacggtt actgaattta gatgtatagt tggctatcca 11580

taaatcacca tgtttaacac gtttattaga aacctgtcgc cgtctatttt tttctagcct 11640

ctcccattta accacatagt ctttatatct gtgcgataaa agactgtctt caataggagg 11700

acactttgcg ctgaatggct ctcctagagc ggctatatca ttcattgtgc ctccgaacat 11760

acatgctgga ttcaaccatt tagagtcttt agctggtaga gtatctctca ttttattgaa 11820

gaattctgtg atttcatttt cgatgtctat ggcgtaactg tggaatagca ataacaataa 11880

tgatacgaaa tatatatgta ccatcatttt catcgtcatc ttcattatac ggcactaaaa 11940

tatttatata atatcagttt ttttacacac atcgcatgag aaaaatacaa ctatactttg 12000

gtaggtggat acgatatatt ataaagatcg ttaattgtca gcatgtataa tatttcgtat 12060

ataatttctg aaggtagtaa tgttagtaga caatttttat ctgtgaaaac aggaaatagt 12120

ttatcatata cctttgacac gtacatatct ttataatata cattaaggta tctctcattc 12180

ataatattag aatatatttt aaaggactta tcgttattat atttttttaa ctcgttgagg 12240

tatcttctta actttttcga attatggcat cttaaaactg catatagtgt agtgtctaga 12300

acagtggata gtttgagcaa tattgtttct ttatgacatt tagttattat accatcgtat 12360

ttatcaaacg acatgtattt tgaaaatgcg tatcgatacg ttttagaatt gcgattccta 12420

acgtgtctat tgtaaaatgt gtcatctaaa agtatacagt aggatatgaa tttctcaaat 12480

gattttatat cgaagatagg tagagatgga agttgatcca ccaacgatct tatcacattc 12540

ttatctacaa tatatctaag tatatacagt aaacaattaa tggtatatgg ggaattttct 12600

ttgttatcta taatgagttg tgtagcgtgt ttgttattgt cggttaacgc atttatatct 12660

ccgttgatac gtataagata atctattacg tgtgtattat tatattcaca tgctacgtgt 12720

aataatgtct ttccttcata gcgggtataa atatctatat ttttaaacct attcatcaaa 12780

taagttatcg aatctgccgg acgtgtagaa tatgttttga taaactcgaa tacgattata 12840

cgacgctcat ctatcggcat ttctccaaca tttgtttcat agtgatgtat taacataaca 12900

agaatatcgt gttgtatgta gtcggaagtt atataacata gtataggcgt taatccatga 12960

ttattacata ttttgaaatt cggattaaaa gtcaacagca ttttagtcat atgaatatta 13020

ttacacattt caatactaag atacaaatgg aatggtgtat taccacaatt atctaacgcg 13080

tttagattag ctccatgttg tataaacaaa gaaataattc gcttggcctt ttttatcgtt 13140

atcggttttc tacactcacc tggtttataa acgtgtgcga gacacaaata ataataatgt 13200

aacgctgtat atccgtcttg tttaaagtta ggatcgattc ccttatctaa taaagtggat 13260

actatgttct catcgatatc ctcttccttc aataacatgt aacgagattt tatatactct 13320

agtagtaggt tggaatagtc tctatgcgat aagtggtttt tatctttgtc tatcatatct 13380

atcactacat catgtgaaat attacaacat ctagtaagca atatataaac aggcatacgt 13440

ccgctactgg ttttcatcgt tacatccact ccatgattta ataatatctt cagtacatcg 13500

tttgtaaagt aattattata caaatagcag tgtagtgcag tatacccagt tacaggttgt 13560

cgtctgttta gatcatagtt tttaactaca aaatcaagtt ctgtagaagt atggtctata 13620

ttgcaatgac taaaatatgt atatatttca ttctcttgta ttttatgagt tgattcgcgg 13680

ttgatattta aaacataaat cagacgacga ctcattttta tgatgctttg tggtaaaagt 13740

cctcatataa ttgtttaata ttcattatta tagacgattc ccattaacta atctaacatc 13800

tttgatatac ccgtaaatat gtaaatatga tcctaaaata acacggattg taagatgtct 13860

agaaagttta tgcaggtgta tgaatatgac agagagcaat atctcgatga gttcattgaa 13920

gacagatata acgatagttt tatcactagt ccagaatact atagtgcgga aaaatacatg 13980

tgtagatata ctacactaaa tcacaattgt ataaacgtac gacgatgcgc gttagactcc 14040

aagttattac atgatatcat aaccaattgt aaaatatata acaatataga attagttagg 14100

gcgacaaaat ttgtttatta tctggatctg ataaaatgta attgggtatc taaggtaggt 14160

gattcagttc tatatcccgt tatatttata acacatacaa gcactagaaa tttagataaa 14220

gtctctgtaa aaacatacaa gggcgttaaa gtaaaaaaac ttaatagatg cgcggatcat 14280

gctattgtaa ttaatccatt cgtcaagttt aaactaacgt tgccgaacaa aacaagtcat 14340

gcaaaggtat tggttacatt ttgtaagtta agaacggata taacgcagat agaggcaccg 14400

ctttcgggca atgttttagt ttatacattt cctgacatta ataaaagaat tcctggatat 14460

atacatgtca acatagaagg atgtatcgat ggaatgattt atataaattc ttcaaagttc 14520

gcgtgtgttt taaaactaca tagatcaatg tatcgcattc caccctttcc tatagatatc 14580

tgctcttgtt gttcacaata tattaacgat gacatagaaa ttcccattca tgatttaata 14640

aaggatgtgg caatttttaa aaataaggag acggtatatt atctaaaatt aaataataaa 14700

actatagcta gatttacgta ctttaataat atagataccg caattacaca agaacatgag 14760

tatgtcaaaa tagcactagg tatagtctgc aagttaatga ttaataatat gcatagtatc 14820

gtgggagtta atcatagcaa tacgttcgtc aattgtttgt tggaagataa tgtataaaaa 14880

ttcttataaa ctcaattgac atggaaatgt aacaacatac attcacgtta tactaacagt 14940

aactgttttg ctgatgctag gaatagtaaa cgccatacac gtaaacgttg tagcatcttc 15000

ttccttgaca ggattaatgt ttaaccggga tgtaataaca cgtctcttat cggtcatata 15060

gattttattt gctacactta ttctaccgtc tccgtcccca tcatcttctt cgtaatacat 15120

accattactt atccaaaaga cgtctgcatc cgttgtggga ggtctcaacg atactctaca 15180

cgcaatagtc aaattactac ctattgaagt tacaacacct tctggtaatt gcatagtaga 15240

aggtattatt ttatcccgta cctctaattt tacaattctg gttacgttat atgttttgcc 15300

accgtatata tattctaaaa cacatgtata ataaccagca tcattttttc taacatcttc 15360

tatggtaata attccaggtg tccgttgttt aagtctctta tttctaaggc gtcgatgccc 15420

gctccatata atatctgcgt ttacgttact agcaataaat gcattaatat tgggacatac 15480

catttcgcca gtagatctct catttactat ttgtggatac gagataagat ctatatttga 15540

ttctgagaca gacacgattg tcaaatttaa cgacatcatg tcacagtagg tttcgttcgt 15600

ggtaatgcat atataaatac cagagtctga ttgtgtcggg ttcagaatta gcatattgct 15660

accattatct atcggtataa ttctatcatt atccgctcct cgtttttccc ataaaatatc 15720

taatatatta tatccggatg atagcgtatt tatttgagga catggtaaga ttactggctc 15780

gttttctaac tccatgaatg atgcaaaata ttgccctttg tcgatacatt caggcgcgtt 15840

aaaagtctga acgaatgaag aataaaaaaa tatagaaaga aatataacag gtagtatact 15900

cattttattt tatagtgggt aaaaaaagtt tgtttctggg aaagggagaa gagaagaaag 15960

aagaaaatgt caagatgtac aactcatcaa ttcatacgcc ggaatatgat gttataatcc 16020

acgtaattta gcatctcaag catcacaaac aatgcgttca gactgttact agtgggatgg 16080

tcttcacctc cccagtaagt agcagcatat gcacaaagtc cgatgatggt tttttgctca 16140

tccggtatat aacaacggat gatttctaca caatctttga catcatccca cggcatattt 16200

tttaccagct cgcgaatgtc gatgaacact aattccgcat catctctaaa agagaggagt 16260

gtggaataca attgtccagc gcattgcctg ttgcggtatg cataggacga ccaataaata 16320

tattcagtca aacattgttt gacgtcatca atactggtca gtctgtcaca tccacagtgc 16380

tgtggtgaaa agacgtgggt actaaagttg gccgtcatct taccaattgc aattggaaga 16440

ataacgacct gcactaatac cacatgtttt ttctattttt ttaaaaaaaa tggttaacaa 16500

ttagttgtcg gagagcaata tctaccaacg aaaaaaattt ttccatcaac atgcctaatc 16560

acatagatga acggatgatc tacacagaac tcatttgtaa ttgttgatgc acagtctgac 16620

accagtgcac aagttgctgc agctgcttct gtatactctt cattgacatc tatatacgtt 16680

ttgtggatca tagcgtcgac actcacatct aaattacaca tattgctata atctccagtt 16740

gaaccgaaca cctctgtcag tcctgacttt actagagtat ccaccagatt atacgagcct 16800

gttaccttaa acttgggaat gtgaacatcg ataaacgtag cttccagaga gttacaccat 16860

ttcttaaaat ttgtatctgt tagattttgt tctatggatt ctaatccatc aatcttgtct 16920

ggaagaatga ccatcatact agtatctcca acatatggca gttctatgat tgaaaagtta 16980

ccgaatgatt cttttacaga tgcgtgatta aatagctcgc cgtacataga catcatactt 17040

acgtctacca tttccgttgg tgatacgtaa aagggataat cactggtaaa ttccttttcg 17100

aatggcgtca accattttgc tttaaagtat acggcactaa ttgctaggag acaattgttc 17160

atccaatagt ggattgattt tcccctcagt aaagatatct acacacttgt tgattgcatc 17220

tatagtgcga caatcagtga agtcaacagt ttgaaactta tcgccaattt ttctcaaaaa 17280

ggaatcttta aacacggcag aatatcgccc atatacttta ttcatggatt tgaatgagat 17340

attctgagcg ctaaccttat ccgtgttctc ctccttttct acatattttg atagctgttc 17400

agcagtggat ccattagctc cataatacag tattgtcaat actgacgaga ttgacgctgg 17460

agaaatgaat acattctctc ctttcataga agatatccat agtaatcgat attggtcgtg 17520

tagcgcgata gagatagtct aatattaata ttagatatcc gtaacactac cacactctat 17580

aaaaaaagaa tatttcaatc ttgtataaac agtctacgta gtctgtcata attaggagtt 17640

tgagaataat ctaacgtgta tactaattct atatatctaa ctaattccag aggttcattt 17700

ccttcaggaa aacagtcctc aaagaaagta gctataaatt ttttatattc ttttttttgt 17760

tttattactt ttatactact ttcgtttttc catggaagtt tgccaccgaa ccattctatc 17820

atgcaatatc ccaacatttc taaatctcct cgttttgaaa ctgttgctcc aagatgattg 17880

tctacacaca tataattgat atttcctgaa gttatcatgt cctcgttgta atctatatgt 17940

gagtttccac tcttgtatag tttgttagtt ctagaatagt caattagtga aagacgttta 18000

tttctaatca gtatattcct cggttctatt tttccatggg taaatcctcg agagtgtata 18060

aactctaacg tgtttatcat agtgacgcat gcttcgaaga ctgattcagt atcctttggg 18120

gcgaatactc ttcccaaatt ctttataacg aagaacatat aatcatcggt ttctccaatt 18180

ccatacagat ctggaatagc caaatacttt ataccacgtt cccgtgtcca gttgtcgata 18240

gtcaaaggat ccaatacaga tatatagaat cgtatttcag acaacaatgg cttgtgtgat 18300

ttgtgatcta tcttcattac gtaattgtag aacgaactag tgaaattttt tctgacctta 18360

tagagtattg aattaccaga atataaagta tttccgataa tccatttctt gccatcgtta 18420

tcaaaacaat acttgaagga ttccatgcct gctatgtaat aacgagttgt tttttatgtt 18480

ttgtattgat tataaaaatt agtagatatg atctatattc ctacatcgtg actgatacat 18540

tctataactt ctttatcaat ggaatcacta ttactacttg tattaataac gagtgcttct 18600

ctaatatatt cattgggatg attcttgtta atgtttttat tattttcaat ttctacattt 18660

tttagtattt cttctttctc tctatctatt tcattcatga tatcttctac atttgtaaca 18720

tctgtatctg tatccatgtt agtagtaagc aataaaacta tagggtaata ctgctataca 18780

taaaaactat ttatattttc attttcaatc aataaggaga agttgataat gcctttatag 18840

cggggagacc atcatacttt tccacatatc caatgtcctt actaaataca tagaagacgt 18900

tatttagttt acacaatgat gatatggata tcttataaat agtatatgaa atatcaaacc 18960

acttattcgt tctaggattg taacaagata tctcgttgtt tttctctaaa ttaccatatt 19020

gttcggtaac ggatagacct ccggcaaaat ataacattcc aaatgtcgag tcgatgccgt 19080

gatataccct agccataggc atttctataa tcgaccattg tttgtcttta taaatgtcaa 19140

gtttttccat attgttaacc acgatcgtgc tattagagtt atttatgaat aatccgccag 19200

ttacgaaaat agtattatta acggaaacag cggacacatt gcttttttta taatttatcg 19260

gtgtttcgta tatccctgaa tccattgcct tcacttgcag ttgggagatg aacactgcaa 19320

tcagttcttt cgggatagaa atctttggca attatacaca accctatttt caatccccat 19380

gtttcctctt cagtcttctc acatcgtcta atagacatgg agtagatgat agaggggaac 19440

agaaggacta taatcaggga cctcatcttg aaaatggtta gagcctataa gggcgttaac 19500

cagtgtataa tatgcagttt tatttcgttt ttgtatcatt aataaaaatt agttatgaat 19560

atttagtcaa gttaagcatg ctaagaaaag tggtaacatc atttgatgtg ctaccgagaa 19620

atttagttaa attttctctt gataactctg gccattttgt ccttgaaatt gggaacatct 19680

tctttggatc taattgcgca tgtatataca ctctttttag gtgtcagaca tactttcgaa 19740

ctccatggag taattttttc gagaaaccct tctgtggctc cctgtgctgt cacgcacact 19800

ttttctgttg tgacaaagtc tatgctgcaa cctgtagacg tgcaatcgta gtcatcgata 19860

tcatatggta ctggttcttt agaatctccg aatgtgaagt tagccgtata caacaagtag 19920

atatcacaca tgtcaccgcg tttgtaaata ggaatctttt tgctacctct agtagcatac 19980

ggatggtcgt cgtattcagt taatgttaca gtcggtggtc ccgtacaata ctcttccact 20040

ttataatcgc cttgttcaaa tttaacccag aaagatacga aatcattctt tggaaatgcg 20100

gatatgtcgt atgttccttc tggtacattt gtatacattg tttgccacgt cttgatgcca 20160

taatttttaa gggatatatt gtatagacca tccccagtcc attcaatttt ggaatcaaaa 20220

ttgacggatt caaacttata actagttatt ttagcgtgta tactattaat gaacaaaact 20280

gcgagaatta taatatatct catggtgttg tttgttattt gactactgtc actgaagtga 20340

taatatattt taaattttta aaaatcatat tttgaataat atgtattact atgtccatga 20400

cgatcaaata tataagtaga tccaatttta agagtatgtt ttctcaatag agtatcatcg 20460

ttgatcatac ttatttcata ttttattcct ttatagcatt ttccctctgc agtaatagaa 20520

tattgtcgct catcaccatc atatttgtga atataagggt acttatcata atggcatgat 20580

aatttggtag atattctaca cgtgtgacct ctgcgttgaa tagtagacat atcaataatt 20640

ttatagtcgt ttataacatc tatatctatg gaatcaaatc cgacgtcgca tgactctgta 20700

ataatagaaa ttgtgtactc tttaaaattt aacaccaatt cattattatc atcagtatat 20760

gaattttcta tattatctac tccatctata tagagtttta cactcagttt attaaacgga 20820

gtgtactcat tattcgaata ggatgctaat gcacctatta caaataaaaa cgttattagt 20880

tttttataca tttaaaatct taaaatttta actagtatat tgaaataatt atttatacaa 20940

actaactaga tgcatcaaca gataataaca aacacctcca gcgatcgtgc caatagtagt 21000

tagagatgcg tatccgtaca acttgttaat catatttcta cggatgtata taccatcgtc 21060

gttaaaagcg cttctatatc tctcattagc tagaattata gatacgctat taattatatc 21120

atacattagt tgattgttga tactattttt attgtaatcg aaaaacattt ccatgaatag 21180

tattccggtg ccaacgctta cagaaacatc gcgttcattc atcattatac cattaagtgt 21240

atccatcacc cattgtttaa cacgtgattc atttggcata gtacgtacta tatcatcgaa 21300

cggtataacg gcgcatcttg taaatagcct gtatatatta tgtaatatgg cgttacttct 21360

tccatacaca ataaatctct tgttataata gtgcctgagt aaaaatgctc taacggcatc 21420

gtagatatca acatccactg aagaagacat ctcaattgat tctagctata agtctttaat 21480

cttttgatac ttgtttgtta ttaaattatt aattattaat tattcattat tttaacggat 21540

ttatattcac ggtagcaatt tatggaactt atattggtca ttatttttgt cacaggaaca 21600

aactaatact ataacggaga ttaaaaatat gacgcccata attgttaacg ccactatgat 21660

tatatgataa gttgcttcta acgattctat ttcttgttca tattgtacaa cgtctttcga 21720

gagtttgctc aaatctgtct catcgtcggg accatcatcc actggatcaa atttttcgtt 21780

agatcgtaca catgttggga gtatgggatt ccatttaccg tcgatacatg tggatgatgg 21840

agatcccgtt agtataaaac cacttttaca actaagatgt ataacgccac cgatagaaaa 21900

tgtagatccg gaaattaatc cattagatag agacggtata tcacattttt gttgacatga 21960

tggaataaca ttccaagaat tagctgtaca acttatgtac gaagcaccaa taacctcata 22020

tccaacatca cagttgatag ttatatattc cccaaatgag tatttttctt taactggttg 22080

acacgatccg tgttctaatt gaagaggttg acattccgca ttaggacacg taacagtatc 22140

attccaagaa gtatttccat ttttttcttc gcaacgaaaa tattttgttt cgccgttgca 22200

acttagtgtc atggtggaat tcacttcgta tagcggttta ttatatagtt cagagatgta 22260

atcagaaact gtgcacattt ttttgcatgg attttcgtat ttccatttat ctgtttcgca 22320

gacagcattt ggatccgaag aatgatatcc ctgatcacat gtaaacgtaa ctttctggtt 22380

attattaaac gatgtttcgg tagacgttaa tttagcgtta ttcatagtgg gtacagtaca 22440

tgttgaataa acaacagcag gtagtacgca taacaacgta acaacggaaa tcgttttcat 22500

ttttatttat gagcgttaaa aatagtatac actgtcgagc actaaaagga aacaatgatg 22560

tagtgtgatt ttatatttta atagtgttta taagattttt agatatgtgg acagttcgtt 22620

atcgttatat tttatttcgt taggaaaaca cgaccattta tctccaggat ccagcttctt 22680

ggataataga ctaattaatc tctgacgata tctaatagat gctatgatat tacgtacacg 22740

acttccgtat acgtcgagac tagtgcactt cacgaaagaa ggatgtttac cgtatctcat 22800

taaagtatta atgtctttga tacaaaaaac taattggaat acagttttat tttttatata 22860

acatctcttc atgatatcta tttcatcgaa acatcttaaa atataccatt tatattgctg 22920

tagtgattgt acatctataa gagtatcata atcggtcata cacgcagtat attttataca 22980

cattttcaat aaatctgcat tatgctgttt atgtttagta attgctatca tagactgtat 23040

cataattttc aaagatggtc gtttagacaa tagtacttcc attattattt tgttgttgtt 23100

tgcgactgct tccgaaatac atgtacatcc actagtagta atcgtctcaa aatcaccatt 23160

tctgtttaat agatagacca acgtattata cgcattataa ctgacagcgt cgtaaatagt 23220

acgatagtta atatcaataa ctcctctgga taatagataa tctaaagatt caacagcatc 23280

aaaaattatt aaatgacgaa ctatatgttc attatctatt tctaactctg tgtctgattc 23340

caaatatagt tttataagcg agataccgtg tataattcca caataaaatg gagtagttcc 23400

gaatttatta cgcgcattaa catttgctcc tttagaaatt aataacttta caaaatcata 23460

tttattacga catgagacat aatgaagagg tgtatatcta ttaaagtcta cagagtctat 23520

attagcattc tctaaacata tctcaaacaa ttccacgttg ttgatatcgt gtttacataa 23580

taggataaaa ggtgtgtttc cgtacgtatc ttttttatct acatctgatc cattatttaa 23640

caatatctta attaattcat tgtatgattc tgattctgat tctgatttct tatagtgata 23700

tatacatcta tgtaataatg tacaaccgta actgtcttcg gcatctatat aaaatacata 23760

tatagatatt aactgtatct gatatatagg acaaaaaatg tcttgttcta cgtccatttt 23820

caagcattag tcttatccta ttatctggat gatcattatt aactaattga tatacatatt 23880

cttttatatt aatggatgat gataacctga gattgtgtat aaatgacccg ttcttattaa 23940

tatttaatac tctatctaga aaaaaaatta taatattcgt attagcatca tccatagaat 24000

aaatatgtag aatattttcc ccatattcta aaatatggaa taaatttggc aacctagata 24060

aaaaatcaac tattgtttta tccactttct cgtatgttcg aacgagatta taatcctgta 24120

ttatatggga tgtggaaaaa ttggaaaaca cgcgtgctat ataatgaaga gataaatata 24180

cactccagtc aagtatttcc tttttaaaaa aaatccatat ataatttata ttctgtaaca 24240

tgttatccct tttcaattaa caatgttggt ttataaaaaa ttaaagaagc gaatcaatga 24300

ttaatagatg ttaagaacta taattacgat gtattaatag gtatagttag ttagttaaaa 24360

agagataaca gttactaatt aattgttagt tattgtctat atgatattac aacctattat 24420

ttgttctcta tagttacatt aattaaaatt ttatatgtga cacctattca tctggagaat 24480

acttcttgat accatgattc tggccaatct gcaaacacag cacaacagca tctttccact 24540

ttgatagcgc acacgtatgt cgaggtagcc tcatccccag gtttatatac cttgatgaat 24600

cgacacgtgt acttgatgtc ctctttcttc tcacagaaat acacaacaca aagtcttttg 24660

atatgctttt ctatatcatt ctctatcagt ctgaggtagt cgtaacccac cgtgaatcca 24720

aatactttgt gtgtattatt atcaactcca atgaacaaat atccaccctc tgtgttggca 24780

aaagatgaga gtatacgtgt tcttagttgc ttagctgaaa cagatgtatg tttaacatta 24840

atagatttac cagcctgaag ttctgatcta ttgaagaact cctctaccaa tctctcaatt 24900

gattcagtgt cttccactcc atctggatat tcaaattcct gcatttctgg tctgggactc 24960

catccacctg attccttcag ttcgctaaga tactcatttg aatgcatgat cccatagtct 25020

tcatgtacaa atttgttttt acagtactcg gtataagatt ttgattagac aaatgcgtat 25080

gcacataaca gcattcttta ccattcacaa tatcaaaatg atcatataca acagaccaca 25140

tatggcgtat agattccaaa cgtttcatga gtacatgatt cacactgtct tccagagagg 25200

tggttacctc gatggtgccg ccgatatagt agaagaaaga ttttctgtat cattctcccc 25260

aagtttaact tttaccagat ttgggatcgg aagcaccgca ccctttttga atctcattct 25320

cataatttcc tttccatgta cgtccacagc agtaacttga cagatacaat ctcctatcca 25380

gacgtggtaa tcgatgatag gtcctgaact gagtgcattc atttctttgg ccttctcttt 25440

gatagtagaa taatcatgtt gaacttttcc atatacagcg ttttctgttt caagtacgtg 25500

atgatgaatc cctactcctt ccaaacacaa gtctagactc tcgtacccga ttccgcttag 25560

agcatagtag tttttgtggc gatatgtgat ttccttcttg atcatggatc ggatctccaa 25620

tttgtagata ttcattgcac tcaaacatag gcagcagtgc tccaatatat ctcttgttca 25680

cctgactgta acaccacata tatttgtctt ttacaatgtc atacttgtta ttataaactg 25740

acatcattgg caacagtcga tgatattcca ggaaaggcat gaagattctc gtcgtaccca 25800

ccgagagcgt gtgcgtaaaa catcgccatg atttcggttg tacacacgag atcaataata 25860

aattaagtta ttttttaatt tttatcgaca aaaattttac atcaaccaaa caccacactt 25920

aataatatac accctgcatt aatatgtgcc gaaacttgtc gtaattgggt tcctcaaaat 25980

atgtcaaaga gtttaccatg gtaatatatt gcagcaattc tctaggtgca tattgcaaac 26040

tggtcattaa caaagtcgca gtattgttaa catatttctg ttttgtggca cttactaatg 26100

cacaattctt tgtttcagat atcttagtcc atggcaagat acctcccaac catctaatca 26160

tacaatatcc aagtgtttct agatctccac gtctagatac aacgtatcct ttatgcgaat 26220

ctataggtgt aaattctaga gtaccgttat ccattttatt tggatttctt ataaatggaa 26280

catgttcgcc attagacatg aatttagaaa ccaatccgta atccactaga tataatttat 26340

tcttatctat ttgatccaag actatattac tcgctttaat atctccgtga gaatatcctt 26400

gctcgtgcat aaattgtatg gtatttaaga tttcgattcc gatcaacatc accgaccttt 26460

ttggtaatct attattattg gctctgatca ccgcatctag atctgcacct aatctattaa 26520

ttaccaagaa tcgatattcc acattaatgg atttgtatag accaaatgcc ttgcacgtga 26580

taagacctac gtgctttata ttgtgagatt ttttccattc ttcgataacg gatggtttaa 26640

gtactctagt ataaaatgcc tgttcggtaa ataatgatcc gttagctttg ggctctattt 26700

ttactacata attattgtca ttagtagtat aaatactacc aaatccacct tttcctatta 26760

atggtccaac gacccattga tttttgcaat tgtcagttaa cacaagtcct tgaaagttca 26820

taatgtgtga tctatctgtc aatgaaatat cattttaaat tttaagtttt acgtggtaag 26880

ttttaatatt taactaatac attagacgtt gaaatagcca catataaaaa cgagttatat 26940

tattaattat caagttttaa gtcttaagtc tctaattagt gttaaaatac attctaatac 27000

ggtcctgtag tatctgaatt aacttactat atgctaaatt cacatcatct tcaatgataa 27060

tagtgtcgaa tagaccggct tcacctgcct catccatgtc agtttttgcc aacatcacac 27120

gacgatgaat ctcatcatcc gcttcagtgt ttctacaacg aagcttggtc tcaaccattt 27180

taagagaggt aggtcttata tacaccgagt aaggcattag gtaagtattt ttaaaacttc 27240

taacaccgtc gatgtttaga tccatcacac aaatacgatt attaatagcc gctgtattca 27300

cagcagtttt agaagttccg taaatatttc ctaaaaactc agtatgttcc ggcggctatt 27360

cccttccaga tggcctctct gttaacgtaa tggtaatcga caccttctcg ttccatagga 27420

cgaggaaatc tagtggtatg ggacaccaca aatccaaata tattcccata gtcttttggc 27480

tatggctgtc tttcctaaac cagatggccc gctcaatatg atagatttta ctatcccaga 27540

catttatgta agtcaaaaat ctagactttg ttctctgttt tgtatttacg tgaacgttta 27600

ttatatatat aatatgtaat acagaatatt gccacggccg acaatataat taatgcggta 27660

ataccaaata tttctacaaa gtccttggtt ttataattgc taatagaggt tgtactaccg 27720

cctacagtag ttgatggtac tgtatcatta tcattgtacg tatcataaag atccgcatca 27780

tcggtggttg atttagtagt gacaattcca gatgatgtac ttactgtagt gtatgagaca 27840

gtgtctgtaa ctgtatgatc ttcttcttta tcagtaattg gttccggagt ctcgtctgtt 27900

gtggattctc cagatgatgc acttactgta ttaatgctat cactagtgta tgtgacggtg 27960

tctgtatgat cttctacatt atcagtaatt ggttccggag tcgcgatttc gaataccgac 28020

gagcaattag aattatctat ataatcaggt ttctcagaac tagtttccgg tgaatgtgta 28080

gatccagata gtattatgtc tatagtcgat tcactatctg tatttacaat caactctgtg 28140

gagtattctt cataatctac tttatcagtg tcatttgtag gcgatgtcat aaagaatgca 28200

catacataag taccggcatc tctagcagtc aatgatttaa ttgtgatagt tgtaactaga 28260

tcatcgtatg gagagtcgta agatatttta tccttggtat aattatcaaa atacaagacg 28320

tcgcttttag cagctaaaag aataatggaa ttgggctcct tataccaagc actcataaca 28380

acgtagtcat ttgtattatt tcgattacat gataaagttg catcatcacc tattttttta 28440

gatgtctgag gaaaaggtgt agcgtatact aatgatatta gtaacaaaag tattggtaat 28500

cgtgtcatat tagtataaaa agtgatttat ttttacaaaa ttatgtattt tgttctatca 28560

actacctata aaactttcca aataccagcc accgaaagag caatcttaat catgtcaggt 28620

tcatatttcc ccaacatact agatccaaat tcgcctacat caggtaaatt catcataata 28680

caatgtctgt tcatatcaca cgatgatcca ttgagcatct catctctatc aagaatccta 28740

atctgtggtt caaaataaca gcatctacac tcatcgttaa ttgtagtatt gtctaatata 28800

tttttgctaa tatttgcgta agttctatta tcagctattg catgcatcac agatccatca 28860

acaaccatat ataatataga acaatagtcg gactttatac ttatgtaaaa cttgaaccaa 28920

ttggaactcg gaagctcgtc atgtagacgc tggtgtctag atataataac attattatcg 28980

gttacacttc ttaagagagg tgccgcatcg atagagaaat caaacaggag aataatcaat 29040

gatgcatttc ctttggtaaa aaaggaaaca tccatgggaa gaatggctac tttatatgaa 29100

tttaactcta tacacgcaca cgctttatca gatgagatta atagttcaca aacatctcta 29160

tcctttccta tggatataat aacaggaatg gcatctttag gtttaaaata attatataca 29220

ccagtaggag tcttgtcatc gtcatctatc tttatcaaat tagcaaatct ggatattctt 29280

gatacattct ttttatacag tgaattgcat acatcggata ccgcattatc catatatggc 29340

aaatctgcaa tcactgtatt gtttttagat tgtccgccaa tgtgaacgtt cttgactttt 29400

tcacaacatg gtttaatcat gaaatcattt tttatatgat ttatttcctc gccatgtttt 29460

actaacgcgt ttagacagta tacaataaca ccatccatgg cgaccaccaa ctaaatagtg 29520

ttattttaat ttgtatgtaa ctattaagat ttagtcaagt ttactaaatc gtttagatga 29580

gtagattctt tccacgtttt atcctctctt atcctagtaa atctaggaaa tctaatggat 29640

attccatttg cggtatggga cttggaagat gtaaactctg ctcctgaaat ttcccatatc 29700

tgagattgtt ttggatcctc tactacaaaa tcgggaatat agattttatt aactactaac 29760

cactctggaa tttttttggg atccttgtta attttaatca tctttaattg gtcttgcaac 29820

tcccttaacg tattatcatc gtgtcctgaa cacttggtaa ccgtcttcca tttaccggat 29880

tcatcgtcgt aacaacccat tagaaagact gccatgatac caccctttgc tcctttacca 29940

tagtaagcac ctagtactac taaatcggca gaatctgcca tggaaccctc gttcaaatag 30000

tctcgcttta tttttaacca tcttctcttt cccggttcgt atactccatt aatatctttt 30060

aagaccaatc cttctaattt tctcgttagt gcatcatcca atacgtcagt taactgagac 30120

tcgttactaa tattcgtcaa ctctgagaat actattctat tgggtatttc aaccataaca 30180

tctttgagaa aagatcttcg ttcgtacaat ggaatgtccg tcatatcgaa tccatcaaag 30240

tacaaacagt caaacacgaa caaacacatg ttagagtttt tatattcttt ctttttgtgt 30300

atacctaaac ttccaaacgg gagcggtaca ttatgttcgt ctacaagaac aatttcagaa 30360

tccaatacga tagacgtagc ttttttaaat gctttcggta tgtattcttt gagataatcc 30420

actttatgag agagtactgg tttcatgttt ctactaaaga aggcaaactc gttattattt 30480

ttatgaactt gtactctttc accatcgtat ttgacttccg caaacattcc tgatggaaat 30540

tttttaaacg ccttattgac agaatcacac gattccgcta acatgggatt aattggagtc 30600

ataacagaaa tagatataga gtctagattt tgtttagatg cattttctat tatctctttc 30660

aagttattag attttctaaa cacatcatag gcatgaggac taatagcgtt aagtacgtac 30720

cgagggcccg ctttaatttt tagatcttta tcaataagca tgactacaca ttttaaatca 30780

ttacatgtac aaacggatgc gatatcagtc aataatttta tttgatgcga ttctttagtt 30840

acggatgata acgtagttaa aaaactatcc acttcttcta aagttaaaat gcttttatct 30900

cgtggacgga tttctgtgtt ctctttgaag aaagtcctaa tagtgtctcc tatatatccg 30960

tatcctaaat cttgtagcat atcttcctga gattgtttaa atattatact atataatttt 31020

ataatttgtt tatcgttcat gttataaatt ctatcgtcta atccgggaag caatagctta 31080

atgatcaaat atttatcatc cctatctgtt ataaagtctc taattaattt agatttttct 31140

ttatatcctg atgcgtgata tatatcacag cataattttc taaattcgcg aagcgacgtc 31200

atttaataaa aaaagtattt ttttttaata ttttcacaaa tatcgttcgc ggatattatt 31260

agacaattgt agtatattct cacgtatcac ttgtttaata tctatatccg ctattctgga 31320

accgagtact tcggcatacg tagttttaaa atctggatta ttcattatat attttagagt 31380

aatgatagca tctactatat tttctaggtt atccttataa aagctcataa acttgttaac 31440

tatacaatcg ataatttcat tgtcgtctaa tgaattaatc aattcggtct gatagatctc 31500

ttgatctata atagtatata cagtatctag gaaagagata aatggattat ccaagtttga 31560

tctacacatg ataaaattta caatgtcctt gtttaatata tcattatcta tagtttctat 31620

cttggcaata actaattgag atattgatgc gagttcggta tgcatatcgg acacgtatcc 31680

gagtactgat tccaagttgc cagagtaata tgcttcatcc atttataaaa aatgtaattt 31740

cactattaca tccacagttg ccccactggt ccagtaaccg tgtgtatagc ctctataact 31800

atattcttaa tcaactcctt ctttacatct tcctcgaatt cagaagaaat aatttgccaa 31860

tgaatatctc cttcttcaat cattttttta tattcttgta ataccttttg ttggaatgta 31920

acatcttcat aaatttcctc gccgacgttt ctattaattt ctttgctacc agattccaag 31980

aatataacta agtcgggttt aggcaatcca gattcataac tcttactgag agtcattgac 32040

gcgcctttag cggcggcata cgctactcca gagaatgcgt atctatcaac tattaaagta 32100

attccctgtt ctagttgttc ttgtataaaa gatgcaaact cccatctatt tgcacaaaat 32160

aatagattaa ctatatgatc attataggtt tttttacgag ttagatagtc atctatcatc 32220

tttccagtga cagtggatct ctgaggaaag ttaagatatt ttatcgtgtt tgccggtata 32280

gattccatga tgttcataca ttgtgttgtt tttccagatt tgtccaatcc ttcaaaaacg 32340

attaatgccc cacgagacat ttttgtaaac ctaacatatt ttttacaatt tatgcgtata 32400

ataaaactga aaataaatat atggtcatcc gagacgatta ggtctacctt tatagatcat 32460

gggtaacaaa aatattaaac catctaagga aaatagactg tccatcttgt ccaaggataa 32520

gatggattca tttaagagag gatcctttag agaaaagtcg cgtgcaacca tccaaagatt 32580

ttcatctctt agacgagaac atattaaagt agaccatcct gacaagttcc tggagttaaa 32640

gagagggata tatgaaataa ttcagaaatc gtcgtctata gatgtggaca aacggactaa 32700

gctcatgtcc aacataaaaa cgatgatgat aaatccattc atgatcgagg gtttaatgac 32760

atctttagaa aacttggatc ccgataacaa gatgagctac tcatcggtga tgatattggg 32820

agaattcgac atcatcaata taagcgacaa tgaggcggca ttcgagttca taaacagtct 32880

gttgaaatct cttctcttgg aatactccat tagtaatgac ttgttgtatg cccacataaa 32940

tgcgttggag tatatcataa aaaatacatt taatgttcca gaacggcaac tgattctgag 33000

aggtcaatac ctaactccaa ttttcagtga tttgttaaag tatgcgggtc taaccataaa 33060

gtcaaacata cttatgtgga ataaacagtt tatcaaacca gtatctgacc tctatacatc 33120

tataagactc cttcattgtg ttacagaatc atataaggtg attggaatgg gataaataca 33180

acaattatat tttttatcat atccttcacg ctataaaaaa aattatacat ccgtttccct 33240

gtcggttacg gcagaacatg tagacgaatc atcgtcttca aaataatcaa attcatcctc 33300

tatgctctca gtgtgcccgc gtagatactt acatagattg tatcgtagtc tagatagctc 33360

agatattgga atactatcag atatatgttc ggctaatgcc atacacctat attttttatt 33420

tgtcaattcc caatgtttag atactagact agatagagct actactattc tagaatcaca 33480

acatatcata tcctttacaa agttgacaaa gtcaaatagt cccgaatcgg agtctacttt 33540

aagtttctca ttaaataata gatcaatgtc tccatactgt ttaatagcca aatcatctag 33600

tgtcatcaat gccgaataaa tattgccgga tatagtattt ctgtgtctta tatattctgc 33660

taaaatcagt ttaaaactat acttattata caagtaagtc atactaaccg gcgtattaac 33720

cgcgcaacca atattagttt cctctggaag cacccctctt atctctatgg agtcattatg 33780

tctattatat gaaataaacg tgtcaactac tttgtcccta tcaaattcga cagtgtagtg 33840

tgtatcatta acttcattac gtatgactaa tttattttgc tgagtagaaa agtaaactaa 33900

tgcatttatt gttttagacg cattaactga tatatcaaac gccattctcg ttaattgtaa 33960

gaaaatgtat tatcttttca tttatgtaag aaataccaat aactccacac gctaatctct 34020

gatcattttt tgaaatagat aacgcctttc caataattgt agatatattt acatctgtat 34080

ctaaataaac atatgctaca ccatatctgt ttacaaagat gttaccgata aatatttctg 34140

gactgcctat ggaatcacat ccttgactaa tatctccgta acgatgaatt atcaaactat 34200

acgttccgga ttttaatcca ataactttat cttttccatg gactggttca aagtaaataa 34260

ctcctctgat attatcgtgg tctattatac aaacagccat tatttagtaa aatagaataa 34320

gtagtctgat attatgagtg gcagcaatgg ccgtgtacgc ggttactggt ggtgccggat 34380

ttctaggcag gtatatagta aaactgttaa ttagtgcaga tgatgttcaa gaaatcagag 34440

tcatagatat tgtagaagat ccacaaccaa taacctcgaa agttaaggtt ataaactata 34500

tacaatgtga tataaacgac tttgataagg taagagaagc gctagatggg gtaaatctga 34560

ttattcatac agctgctcta gtggatgtat ttggaaaata caccgataat gaaatcatga 34620

aagtaaacta ttatggaaca cagactatat tggcagcttg tgtggaccta ggaatcaagt 34680

atttgatcta tactagtagc atggaagcaa taggacccaa taaacacggt gatccattca 34740

tcggccatga gcataccctt tatgatatat caccaggaca tgtatacgca aaaagtaaac 34800

gtatggccga gcaactggtt atgaaagcca ataattccgt aatcatgaat ggagcaaaat 34860

tgtatacttg ttgcctaaga cccactggaa tttacggaga aggagacaaa ttgatgaaag 34920

tcttttacga gcaatgtaag caacacggta acattatgta tcgtacagtc gatgataacg 34980

cggtacatag ccgggtatat gtaggaaatg ctgcatggat gcacgtgttg gctgcaaaat 35040

atatccagta tccgggatct gagattaaag gaaatgctta cttttgctac gattactctc 35100

catcgtgttc gtacgatatg tttaatcttc tattgatgaa accattggga atagaacaag 35160

gatctaggat tccaagatgg atgctaaaaa tgtacgcgtg caagaatgat atgaagagaa 35220

ttctatttag aaaaccatca ctactcaaca actatacgtt gaagatatcc aacactacat 35280

ttgaggtgcg taccaacaat gcagaactag atttcaacta ctcccctatc tttaacgtcg 35340

atgtggcatt cgaacgaaca cggaaatggc tagaagaatc agaataagta ttttttttta 35400

aaaaaataat cgagtagggt atcaccacta cacagtaaga gtacagtaaa taaaaagtat 35460

ttatatactt atttttatag atcgtattat gcgctttcta catccatatc atcgtcatct 35520

tcaatatcga taatattttt ttcttctacc ttgacatctt ttgcctcttc gacagcaact 35580

gattgcgcta ataatacatc cttttctttt ttctttttag gtgtgacatc aatagttatc 35640

ttttccaagt tatacgatat ggtaacaaca ttaccaattt ttgaatcaac cgtaattttg 35700

attttatcca tttctaatag catagcataa ttaagagttc atttttattt ttttaaacac 35760

gtatatggtc actatcaatg tagacgctat tagcatacat attgcagtaa aatcatagat 35820

gtcatttata tctttatacc atataataga atcgtatcct attatagtaa tacacgtgga 35880

ccgatgtaga gaataagtct tatgtttaga tgttaatata gtagatgtac tcgttgccaa 35940

atttttaatg agacacgata catttactga gcattgtata tcattatatt ttaagtttcc 36000

tgataatgag acaatcaaac taggattaag tgaaccatat ttgtgtttta aaaaataacc 36060

atgtgtaaaa aagacattat cgtctagttc atctacggtt aacgtgcgat taataaaatt 36120

atatctatat ggaatatgcg gattatattt tatatttact acattataga tcctatcaaa 36180

atacaaatgc ccataacata attccacatc ctctgaatga aatctaaaaa tcgatgaact 36240

gtatgccaat accggcatta ttgacatcgt caatatggat attatccatt tcatcatcat 36300

catttaaaat tgacactaca tatgaatatt actttttatt ttaattacca gttgcacgta 36360

cacgggtagc gtgatctcta cacgttctat acacgtcacg tctggatgtt gttggcttac 36420

ccatcaaaaa tataagtgcg gtatgagttc tcgctataac gatcggagat acaggggcgt 36480

acccagtaag caactccata gcatacgtgt tctcatccat tagagagtta gtatatacaa 36540

tacaatattt ctgaccaata agaggtttta gaatattacg attagtgatg agaggattaa 36600

tcttggcaaa tgttctgtta ggaatagcag ctagaacatt ctttgtagtc ttgtaatcaa 36660

cgatggcggc atcctcgaac ttattatttt ttgagatatc ctcgataatt ttatgccatt 36720

cagccattgt tatttattat atatgttttt taataagaca tctattggaa taaacttgac 36780

attagcattc tattcttact acaaaatata aaatataaaa taaaatatac aatccaatac 36840

tcacataatc caactcactc gaacactatt tttccaatta cgataacaat attgcagaat 36900

gtactcgtta ttatttatta ttttgatgtg tataccattt agttttcaaa cagtgtatga 36960

tgataaatcg gtatgcgatt ctgacaataa agaatatatg ggaatcgaag tttacgtaga 37020

agcaacacta gacgaacacc tcagacaaac aacgtgtgaa tccgaaatcc ataaatatgg 37080

cgcatctgta tcaaacggag gattaaatat ttctgttgat ttattaaact gttttcttaa 37140

ttttcataca gttggtgtat acactaatcg cgataccgta tacgcgaagt ttgctagttt 37200

ggatccatgg actacggaac ctataaattc tatgacccat gacgatctag taaaattaac 37260

agaagaatgt atagtggaca tttatttaaa atgtgaagtg gataaaacaa aggatttcat 37320

gaaaactaat ggcaatagat taaaaccaag agactttaaa actgttcctc cttctgatgt 37380

aggaagcatg atagaactac agtctgacta ttgcgtaaac gatgtgactg catacgtcaa 37440

aatatacgat gagtgcggaa acattaaaca gcattccatt ccaacactaa gagattattt 37500

taccaccaag aatggtcaac cacgtaaaat attaaagaaa aaatttgata attgttaatt 37560

gttattttta taaaaacaag aacggtacgg cgatatttat ttttttctaa aacatctaac 37620

cgaagtagtg gtatgataaa aatgtagttt gggagtattc gttgtactac aaatatattt 37680

ccgtttttta gttccattct tcgtggcatt tttagctata aaattatatg gggttgtctg 37740

gtttaatatt tcggattctc ctacccaata tccgcgtcta atgcttctta aaaaacttaa 37800

ctcgtttgga gtctctatct taattagatc cgaatttgga tttagagctt tgcatgcatt 37860

acgtccttcc tcccaggttt ttcgatcagt agataaatgg atacatttat tattatagct 37920

tatccagtca gtaggacaat cttctctaat atatgcatct tttatcgttt tatctattga 37980

tggtgtatga actaatttac gttctacaac ttttaatagt gtcgctgtaa aaataattcc 38040

aacaattaga ccgcatatta cgcagcaagc ataaccagca taatctgtct tatgtttgtt 38100

cattgtcgtg cggtgtactc gattaagatt acattttaag tatttttttc atttgtggca 38160

ttcctactcc tatccctata tctgctataa ataacggttt cgaagttacc attactgaaa 38220

atagtcgcgc atcgacttgc gagctacgag tttgtttaga ttttgtatcg aacgaatata 38280

tagaattttc ttttatgaag tatattctat attcccgatg ttcattttcc ttctccttta 38340

tatcgaacca tttaacacca gacggtcctt cgaagatagg ttggttagat aaaggcttta 38400

taatatcatc tagtacatta tatttttcta tgacttcaaa cgtggtatgt ggaacaactt 38460

ttccagctgg taaacatata ccaggagctg gagacggcaa ttgctttgta tatccttcca 38520

attttgacgt agaaaaagat tgtttaatgg tattcataga ataggtacat aatgcggact 38580

tggaataagg actatcgaag aatacatata gtatcgtatc attatctgtt tttatagttc 38640

tagaatgaat aatttgtcta taacttcttc cgtcgatatc acattctaat tcgactttga 38700

gaaacgtcga ccatctatga ctagacaatg atgatggacc accttcgtcg tttaggcaca 38760

tttgtgctat atacggaatt ttgacaattc tctttgagcc gatagtatca gtgaaaagaa 38820

tgtaaacttt gtcataagta ccatctttat cgacgaatgc tcctcgtaaa ccatcttttg 38880

gaattacgtt atccgccgtg tataaatcat aaccacatgg tccgtcaaat cttctccatc 38940

gtttaattcc ttcttttgat atgtttatgt cagatagtac acatccgttg tgactgatta 39000

tcgttacttt gctattttga taaggagcgt atcctctacc tctatgtttt gggtcgtctg 39060

aaccgtctat tttccaacat ttgggatttc cgttattggt tccgcatcct ctacttttat 39120

agatgttgtt atataattat tattagttaa accagttttg tttagtttat tatttgaaaa 39180

tgtgtatacc gccccattaa cacccgtgta taatacgtcg tctaataagt aagtagaaat 39240

tatttcttca ctcgtttcaa acttatgcca ttcaataccg ttagcaaaat agaataaaat 39300

aaataacaaa ggtatcattt taaataaata aaaaatgtca agagttagaa tatcgttgat 39360

atacctctat acgttggttg ttataacaac tacaaagacc atagagtata cagcatgtaa 39420

tgataccatc attattccgt gtactataga taatccgaca aagtatatta gatggaaatt 39480

ggataaccat gatattttaa cttataataa aacttccaag acgacaatat taagtaaatg 39540

gcatactagt gctagacttc attcgttatc agatagtgat gtctcattga ttatggaata 39600

taaagatatc ttaccaggta cttatacatg cggggataat actggaataa aatctactgt 39660

gaaattagtt caacttcata ctaattggtt taatgattac caaacaatgt tgatgtttat 39720

ctttacgggc attactttat tcttattatt tctcgagatc acttatacat cgatatccgt 39780

tgtattttct actaatttag gaatcttaca agtatttggt tgtgttattg ccatgataga 39840

gttatgcgga gcatttttgt tttatccgtc aatgtttact ctccggcata ttattggatt 39900

gttgatgatg acgttaccat ctatatttct tataattact aaagtatttt cattttggtt 39960

actgtgtaaa tcatcatgcg ctgtacacct cattatctac tatcaattgg ccggatacat 40020

tttaacggtt ttgggtttgg gattgagttt aaaggaatgt gttgatggta ctctgttatt 40080

atctgggttg ggaactatta tggtgtctga acattttagc ctgttatttc tagtctgctt 40140

tccgtcaacg caaagagact attactaggc atgtgtataa taccacgagc tagtagacgt 40200

ggaagaggaa ccaggaacta ttgttatttt ataataatcc ttgatcttat tcttattctt 40260

tttatcagat tttttgcttc cagttttatc agtagatata caacagagtt tcataaaaag 40320

agaagagaat gaatccattc cgtacagtat ttgataatag cttttttttc gttttatgag 40380

gtatttagag attagagatg attaatgatc cccatactag aaatctatta atccacagaa 40440

atactaatgc tcatatttat attatcaatt ctaccgtaca atatttgttc cttgataggt 40500

acagaattgt acgactgcat ttttttgtat actaagtcga atggatgctc gaggttacaa 40560

acatgtatta aagtatcttg ctcgatgcta gaatttatgt acttggcaga ggacatacct 40620

atacagtttg atggaatagg aaactgctta ttgtcgatgg ttataatgga ataccactta 40680

ttattcgcaa atatctgatc gcatatgtgt ctatccaaat agtcggtaac tcgtctaata 40740

atgaatacag acaacggcga ataattaatc agtgatagat tgtttacata cagtatgtcg 40800

tttgtaaacg atgttaccaa tcgtttgcta ggtatatccg ctttaacaac caatatggtt 40860

gtaggaccct tgctaactac gacacattta ctcagtgggg atatgattac agcatgatca 40920

atgggaaagg ttagaatatt tttgccattg acgtcattat ctatagagat aacacatcct 40980

ccgacagaac taatacagag tataatcgtc gataatctat gaggaataag atggtacatc 41040

tcattgtcat ttacaacttc tacgcttaag atgttggtaa gaagtaccgt ttcatccatc 41100

tgtctattga tctcatcaga tataattttt tgatgttctg tatctatata atatctacag 41160

tcattattag caataaaatt gctaatttta gaaatgccga atacagggaa tatctccatt 41220

tgtacaacgt gacggcagca ataatactaa atattacttc aattttataa aaaggaaact 41280

aatcacacca atgattctat atcatcgtgt tcatgatctt gttcttgctt attaagataa 41340

tcatcgctat gtgctcgtct aaacggatta ttctcactaa atttattgtt gagttctgta 41400

atgaacggat ttgacttgct acaaatactg gttttattat aatttacgaa aggattggat 41460

gaatagttag gattctgttt ggtatcttca ttaagtacta ctgtagtgtt ctgataaata 41520

gtctgttcat tacgatcatt atttattagc agcgtgcttc cagcaacact atcgtaaatg 41580

tgttctgtgc taggcgccat aacattggat tcgttatccc atattaaact accagcgaag 41640

ctatcatcgt ccaatatctc attcctagaa atattatcta cgtcattgtt ttgttccata 41700

gcactacagt gatcctccca gtctgattcg ctatcagttg atttactaga tttgctggaa 41760

ttagaactct ttatcttttt taattttgtc atgataattt tattgtcatt atagacagta 41820

cgtatctttt tcctacaaat atataatata taacatacta atattgttcc cgcaactacg 41880

gtcaccgtga taagaggtac cagcatcatt tctgattgct caatatacgt actactagtt 41940

caatatttat tgttttatta tttactttac aacttttatg ataaaaaatt actattgcat 42000

ttcccatcaa ttatgatcca tccattgccg tctgatatgc agaatgaaga attaaacgac 42060

aatttcattt tcagattatc tgaaggattg atgattagat gataatctcc agctgtatac 42120

aggttatacg cttctatgat tgtagcgtta aaaacatcaa tagatgggaa tagataaaat 42180

ggctgatgtt tatcttctat atctaacatg atatcatagt cgcatgctat gatgatggta 42240

ttttttgatc tgtatgcggc acaaatgcta taaatgttga atctagccaa atcattagaa 42300

tcctgaggaa ctattttacc cttatagcta aagtagcatt tttgcatgtc ggaatatttt 42360

atatcccgta catctatttg gacagttttt atgttaccta taatgtatgg tcctataaag 42420

tccatgattg agatatcgag atcatcatac aatgtatctg ttatagtcaa cacacccatt 42480

ggagtaataa caaacgcggc gtccatggcg gcgtacgtta acgacttatt attaattcat 42540

tttttgttgt cacttgtaga attttttaac acatagtaca gattgagtac ttttacatac 42600

tgttttaacc agttttccag acttgtatat ataacacgct tcagcatccg tcgtactgtt 42660

tagttgttta aaatttgtta atttactaat atctatatct ttatcattat taatatctaa 42720

ccatttatca ttggtctttt ttaaacttac ccaataatct ttataaaaaa tactaaacaa 42780

tactctcaga tgtctagtat ccggtctagg caatctggct cgtaatttac gacactgata 42840

aaccgcatta tctgtagaca ttttaatgtt agtatctaaa taacaatgtt tatcgtattg 42900

tatccatcca ttggcgcaag cactaggcat cagttcttct ttgtaatgca gaaatgttcc 42960

tatgccacta ataatggttg agagtatcat cattatagcg accggcaccg acaacttctt 43020

aaacctactt acagtttgtc tattaagcga tttcatttat taatgtacaa aaataaatat 43080

tagttcattg ttttaacaca aaaatacttt ctaacttctt gtgatacatc agaatcttga 43140

taatcggatg tagtttttgt aattggatta ccatcagatc cccatgtatc cttaacataa 43200

tcaatgagcc aggtagtcaa gacatcggat ttattgggta gtgttgatga ttccgcagtg 43260

caatttgctt tagcatccga gaataactgg tagtctgaat gtaatatata acaagaaccc 43320

tggtaatata aaccattaca gctttctttg tgatcatatt gcgtagtgct agacgcaacc 43380

tttctatgag tagatgatgc agcagcaacg gcaacagcgg cgtcagtaat agcagcctcg 43440

ttagcagaca tgcattgatt taggcgcact atgagaaacg cggacatggt aatcatagat 43500

agtagtgaaa taaccataga tattctaata catagaccaa tcacgcgttt gcgtttattc 43560

tttccctgaa ttttgtctct gtaaacagta gcggagaaca cagatgtctg ctcttcgtcg 43620

ttttctggtg tcatcatgat aataaatgtt atttatgtca cgatgtgcct tctttgttct 43680

cctccctact aacgacctta gttttccata ttttgattta ttatcaaatt aatttagtaa 43740

ctgtaaatat aattatgaat tgtttccaag aaaaacaatt ttcaagagaa aatttattaa 43800

aaatgccgtt tagaatggtt ttaacgggag gatctggatc tggaaaaact atctatttac 43860

tatctctgtt ttctacacta gttaaaaaat ataaacatgt attcttgttt acacccgttt 43920

ataatccaga ttatgatgga tacatttggc caaatcatat taatttcgtt agtagtcagg 43980

aatctctaga atataatctg atacgaacta aaagtaacat agaaaaatgt attgctgtcg 44040

cacaaaatca taaaaaatca gcacactttt tacttatttt tgatgatgta ggcgataaac 44100

tatcaaaatg caatactcta atagaattct taaactttgg aaggcattta aacacgtcta 44160

ttattctact atgccaaact tatagacacg taccaatatt aggacgggct aacattacgc 44220

atttttgtag ttttaacatt tccatctcag acgcggaaaa tatgctacga tcgatgcctg 44280

taaaggggaa acgaaaggat atattaaaca tgttgaatat gatacagaca gctagatcca 44340

ataatcgatt ggctattatt atcgaagact ccgtattttg tgaaggtgaa ttacgtatat 44400

gtaccgatac cgccgataag gacgttatag aacaaaagtt aaacatagat attttagtaa 44460

atcaatattc gcacatgaaa aagaatctaa acgctatatt agaaagtaaa aaaacaaaat 44520

tgtgcaatag cgatcaatca tcatcgtcaa aaaatgtatc ataattataa ttattaactt 44580

ttgtaacaat agtcctattt agagaaagtc tatcgataga cgatcccaat ttgtaaattg 44640

ttccactact tgtcactcca tgatatgata aatccatgta aaatagcatc atctttagat 44700

cattaattgt taccttcccc aatacaacca aatcatcatg atatatacct cctccagaca 44760

agtatttaac aacggtagaa tgctttggct tataaaatac aaatgacatt cccttatgtt 44820

taatcttaat cttttcttta gttattgaat cgttacaatt ataaaatgat gttttttcca 44880

aaaacctaag tgtatttaaa atagatgcca tgttaaaaat gtccgccgtc gactttttgg 44940

aacgattgat aaaagctggt gtttatattt atgttttacg gacaaagtgt gtaattgcag 45000

ctttactagt aaaaaactat tccataaaag acgaataaga tacaaacaca aatgtttata 45060

taatatttaa atggaagacc ttaacgaggc aaacttctca catttattga taaatttatc 45120

taataataaa gatatcgatg cgcaatacgc gtctacatta tccgtggtac atgaattgct 45180

atccgctata aattttaaaa tatttaatat aaacaaaaag tcgaaaaaga attccaaatc 45240

aatcgaacaa catcccgtcg ttcatcatgc agcatccgcg ggaagagaat tcaatcgtcg 45300

ttgaactcga accctcattg gctacattta tcaaacaagg atttaataat ctcgtaaaat 45360

ggcccttgtt aaacattgga atagttttgt ctaatacatc taccgctgtc aatgaggaat 45420

ggctaactgc ggtagagcat attcccacca tgaagatatt ttacaaacat atacataaga 45480

tacttactag agaaatgggg tttttagtct atttgaaaag atcccaatct gaacgcgata 45540

attatataac tttatacgat tttgattatt atattataga taaggataca aattctgtaa 45600

ctatggtaga taaaccgacc gagttaaagg aaactttgtt acatgtattt caagaatatc 45660

gtttaaagag ttctcaaaca atagagctta tagcgtttag ttcaggtacg gtaataaacg 45720

aagacatagt ttcaaaatta acatttttag atgtggaggt atttaataga gaatataata 45780

atgttaaaac tatcatagat ccggattttg tatttagatc tccatttata gttatttctc 45840

ctatgggtaa actaactttc ttcgtagaag tatattcgtg gtttgatttt aaatcgtgtt 45900

tcaaagatat tatagatttc ttagaaggtg ctctaatagc caatattcat aatcacatga 45960

ttaaggtagg taattgtgac gaaacagtat cgtcttataa tccagagtct ggaatgttgt 46020

ttgttaatga cttaatgact atgaacatag tcaacttttt cggatgtaat tctaggttag 46080

aatcatacca tcggttcgat atgacaaaag tagatgttga actatttatt aaagcattgt 46140

ctgatgcgtg taaaaaaatt ttgtcagctt ctaatagatt ataaatgaac tctctatcaa 46200

ttttttttat tgtggtagct acggctgcgg tgtgtttact ttttatccag ggttactcaa 46260

tatatgaaaa ttatggcaat attaaggaat ttaatgctac tcatgcagca ttcgaatatt 46320

caaaatctat aggtggaaca ccggcattag ataggagagt tcaagatgtc aacgacacaa 46380

tttctgatgt aaagcaaaag tggagatgtg tggtttatcc aggaaacggt tttgtatccg 46440

cttccatatt tggatttcag gcagaagttg gacccaataa tactagatcc attagaaaat 46500

ttaacacgat gcaacaatgt atagacttta cattttctga tgttattaac atcaatattt 46560

ataatccatg tgttgtacca aatataaata acgcagagtg tcagtttcta aaatctgtac 46620

tttaaatgga cggaactctt ttccccggag atgacgatct tgcaattcca gcaactgaat 46680

ttttttctac aacggctgct aaaaagccag aggctaaacg cgaagcaatt gttaaagccg 46740

atgaagacga caatgaggaa actctcaaac aacggctaac taatttggaa aaaaagatta 46800

ctaatgtaac aacaaagttt gaacaaatag aaaagtgttg taaacgcaac gatgaagttc 46860

tatttaggtt ggaaaatcac gctgaaactc taagagcggc tatgatatct ctggctaaaa 46920

agattgatgt tcagactgga cggcgtccat atgagtaact taactctttt gttaattaaa 46980

agtatattca aaaaatgagt tatataaatg gcgaacatta taaatttatg gaacggaatt 47040

gtaccaacgg ttcaagatgt taatgttgcg agcattactg cgtttaaatc tatgatagat 47100

gaaacatggg ataaaaaaat cgaagcaaat acatgcatca gtagaaaaca tagaaacatt 47160

attcacgaag ttattaggga ctttatgaaa gcctatccta aaatggatga gaataaaaaa 47220

tctccattag gagccccaat gcaatggcta acacaatatt atattttaaa gaatgaatat 47280

cataagacca tgctagcgta tgataatgga tcattgaata caaaatttaa aacgttaaac 47340

atttacatga ttactaacgt gggtcaatat attttatata tagtattttg tataatatct 47400

ggtaagaatc acgatggtac tccttatata tacgattctg aaataacgag caatgataaa 47460

aatcttatta atgatcgtat caagtatgca tgtaagcaaa tattacacgg tcaattaact 47520

atagctctga gaattagaaa taaattcatg tttataggat cacccatgta tttatggttt 47580

aacgtaaacg gatcacaggt atatcacgac atatatgatc gtaatgccgg ttttcataat 47640

aaagagatag gtagactact atacgcattt atgtactatc tatctatcta taagtggtag 47700

atttttgaat gatttcgcac tattaaagtt tacgtattta ggagaatcct ggacatttag 47760

tttgagtgtt cctgaatata tattatatgg tttaggatat tctgttttcg atactattga 47820

aaaatttagc aatgatgcta tactcgttta tattagaaca aacaatagaa atggatatga 47880

ttatgtagag tttaataaaa aaggaattgc taaggtgaca gaagctaaac ccgataacga 47940

taagcgaatt catgctataa gacgcatgaa ggctgaacgt gaaatcgctc gtaaaaactg 48000

cggaggtaac ccatgcgaac gtgaagaggt tggaatatca actagatgcg gagaaagaaa 48060

aagttaagtt ctacaaaaga gaactagaac gtgatcggta tctttctagt agatatctta 48120

cctcttcttc agatccacat gagaaaccat taccaaatta tacatttcct cgcattgaag 48180

tagctccgtt gatgactgag gataaagaac cagaacctgt agaagtggtg cctccatcgt 48240

ccacagacgt taccgaaccg attagtgatg tgacaccatc ggtggatgtc gaaccagaac 48300

atcccccagc tttctgaata tcagacttca gtatcccaag tagcagttac acctccacca 48360

aaacctaaaa ctccacagat tttcgaatat cagacgtccg attctatagt taacaatcca 48420

cgcccatttt ataattcgga tctcgaattt gatgatattg atatgtatct actaccaaac 48480

tagaatatta caccagaaaa gacggtttga gatcaacttt atctaatggt ttataaaacg 48540

aaggaggcct tcgttcgaaa tctaatttaa cttttacgcc tctggcgttc atttgagtaa 48600

gaaatacttt agatacgtgc gtggtatcaa tttttgttaa gagaggagag agattaagtt 48660

ttgaacatct aagacatgta ttaataccct tgatttgtgc tgctatgtct ccacaatttt 48720

cacaaacata cacatcttga taatcttctt ccgaatcttt caaaacttct gtaatagtat 48780

tggctgcacc atgcgctatt aaacagtctc tctccatttc tccgaacttg atacctcctc 48840

cacgttttcg tccctcattc gcctgtctaa tgagcttcgt ctttttacct ctacatctaa 48900

cggttgcctt gtcctgagtt aaatgcctca gacgcaagta ataaattggt ccaaaaaata 48960

ctttggatgc ataaggctta tccgtttcag gatcatagag aatcttttca caaaagattt 49020

tatccgataa ttcttcatca gacaatttcg gatttgaatg ctcataacat tgtttagcga 49080

attgcatata tgtatcgatg gatgtttcgt tactactagg aaaacagaca ggtcggtttt 49140

ctcccttatt gttgtacggc ttagcagaat atgcggctgt taaaataacc tctatcaaca 49200

tagatatagt ttttctagag aagatggatg tagaattaat aatgacatct ggtgtgatac 49260

cattttcatc gtatggaagt tccgtttcat ccgcgatata cgcgactgtt cccttttgac 49320

tcgttctagt ggtaaatttg tctcctagaa tgggtcttct ttctttcatg gttaatactc 49380

gtaccttaac tttgtcagta agttctactt gtactcgttc aacgcgagat ttgtacatat 49440

cggtatattt ttcggaaaca tcaaagctaa tctgattatc tctagcaaaa tcatcttcaa 49500

gagttcttga tgagatattt cgtgctatag cgtctccgga ttccaagaaa gcattcagtc 49560

taactaatcc attactttct aattttgaat aggcgttaga cctatctctt tctttgttat 49620

taaagttttc caacggaatt tctacttgat gtttcttggc tgtaacaata tcgagaccgc 49680

ctctctgaat aaattgtttt ttgatgataa ttccatcctc ttgattgata cctttgtacg 49740

acattaatgc tatagtaaca tgttggccga agcaattagc cgcaatcttt gaagtttcta 49800

aagccttact aatcacgatt ggcctctctg gatacatcaa atgaattcca ttgtctattt 49860

tatttcgtat atccgagctc agacaagaga tagcttgttt agcttgagca catccaagaa 49920

tagctctggg tccagaattg tgattgattc ccactagtga agatgctaca tatccatctc 49980

taaattcggc aggaaagtca cataaatcgt attgctttct ttcatccttt gacatcattc 50040

taaatttttg aaccgattca catacgttac taaaagtaaa ttgttctata tctaccattt 50100

cgatgacatg cggaaactct ttctgaatgt cagagaatgt catgtcgtct aatctgcttt 50160

ctaactccgg acacacatcc atcatgagct ctccgttatc cacaaccaag aatggtctga 50220

ctaatcttcc cgctccaata ttaatgcgaa tttcattcat gtgatcccta actaaagtaa 50280

tacctacctc caagttaccg aagaatccca tccgttttct acgtctaaag tcagttacaa 50340

aatcacatat catatttgga ttaagagatg cgactagagc attttctata gtgattggaa 50400

atcctgtttc aaagtaactt atatcatctt tataatatga tctgatatac tcacaaattt 50460

tcttttccaa atccaaatac tcagacgtta gtatatttgt aatggaactc aagacagaca 50520

attgagatac taaacctacc tgaggacctc tttccggaac gtccgatgaa caaaagtaaa 50580

gatattgact tggatggtat tttctgacag aaaacatctt tgaaatcttt acttgatccg 50640

gataaaatcc aacactccta ggaatagaaa tattctgcat ccatgaatag tgaggatgtg 50700

ttcgataact accgtctgac tttttgaact ttccactcaa tagactagaa aatgcatgat 50760

taagtccagg agttgttagt acatgaatgt ttaccgcgta agtgcctctg ttcttgtgat 50820

tgttcatgat atcgtttcgg atgttgccta tgtaattctc taattcatca tgtgccaacg 50880

tctcaaaata tttgccgtac gttaggatgc gatgacaaac catcgaatct ctatctggat 50940

atctagaagt gtggtagata cagtagagaa actttcttag taaagatatc atataaaatc 51000

cctttagttg atcgggtgta tagttcatat gaggtaaaaa gttatgcagc atttcatatt 51060

tgaactcgtt gatcgttaac tgagattttt gtttaatgtg ttcagcttcc accaggtcat 51120

taatgtatgt atcaatatta aagtcagtaa tagattcagt aattcgttta gcactatcta 51180

ttatcgcgtt tactagatag acaacttctg gaggcatatc gtatgataaa ctacgtctaa 51240

tgaattctag actcactcta gataaatatt gagacagcaa aacaatattg accgttataa 51300

atgtctttgt cgatgatata ttcaacgctt ccaattgtga tatatccaga gatatcttgt 51360

aatggcgata tcttgtcggt aatacattag gaggggatac ggaggaaaac gagaaagtga 51420

atgagtttgg cttaacaacc ctaaattttg gccatgttgt aatcttttct actagattaa 51480

ttcctacttt ttcgacagat tgtttattga taaagactcc acctatgaca ttaggaacaa 51540

gatacttggc tgtatcaagt ggatttttat tcccgtatcc tattagtagc ggaattttta 51600

tcaaatgaga atcttttccc tcataactac ttattttagt gatggatata ccctctttgg 51660

tcattacctc tttaaagata tttacagtga acgtggctaa tgcgtcataa ctcttacctt 51720

taatactcgc gattataggt gagtaatctg gaggagtcac tttaacattg ctaatttcta 51780

tcataattcg ttctgtatta ttcttaaacg ataagagtgg ccgcttgacg gtcaagattt 51840

catgcaatcg atgcaatata aaattagaat acgatacata ttggaaatgt aacggtctat 51900

aaaaaactcc ggctttagga tcaggcacca aaaacttata tccgagtcgt tgatccattt 51960

ctgaattagt gttttttttt cattttagaa gcaattcttt tagacgatct accgattccg 52020

tttctatatc tatgctattt aaaatagtcg atctttcctt catcatttct atcatattct 52080

cggatttagt cacgtaattt atacaatcct ccagagatcg taatgctaca atattgagct 52140

ttttcccctt aaaaaacttt attttgctat cctcatctaa cagagagggg aaaacggtat 52200

gatccaacga cttaataaat gtcaggttaa acatccctat tttgaaattg ctaactccat 52260

aaacatcgtg ttcagccatt ttcttaatta ggaataattt ctcatctaat gccgtagccg 52320

tgagtacgtg gtttacaaca ttagctactt ctttaaagta ctcgttttgg atgatatcct 52380

cgaacaacag aaataaatta taatagacat agaataattt gtcaatcatg tgtatacacc 52440

ccttttcatc cttgtccttg gatagaataa tgtgcatagt agaatccttg aatattttca 52500

cacatgttct attagtaaaa ataatttcta tagtttgtaa tatagagctt tttccacgcg 52560

ttactataga gtgattaatg atatctactc tcattctctt agaaatggtt ttgatccaag 52620

gttgattaat gttggacctg actgtagtgc tcagatactc atcagacttt ttaccggaaa 52680

agtaatttgc attagtgcct ccatgtacag ttttctttat ttccccataa ttgacctttc 52740

tcttcttatt ggtagtttta atatatgata caagagatga cttttccctc tcaaatatct 52800

ctactgtacg agagctaata ttgctattat ctgagtttat atcgaaataa tgacaacatt 52860

tcacttgtga agaatcgtta aataccggat acgtattctg attaataatg tgtttagtaa 52920

gttggatgtc gcaatacact agtttcttaa tggcatgatg tatatcttta ttatacacta 52980

tattatcaaa catatcctct gaggaaatgc gttctttcat aagaccatat atatctccta 53040

tttcatcgca acttattaga tgaaagttaa aaatagttct ggcatatcta tcttctattt 53100

catgtagaaa ggtaaataga ttatccataa tggcgttact aacgttatta catttttttt 53160

atgtaatttc tagatttaca cctattataa ggtgtataat tagtattcca tttatctaat 53220

acgtatctca tagccaaatt gaaactatcc gctacatcgt ctaatttgcg tctatccgga 53280

acggagtctc gcaatccgaa tgtgtccatc caatcaagaa atgcttcgac cgatctcttt 53340

tttcgatctc tatatgaatt accagacatg acaggcgaga cgcaaataac tttggcagcc 53400

gatgtatgat ataaaaagcc tttaataaaa tagataaatt tgacgtacgg cgaccttcta 53460

ggctgacgtt ctagaagaac tgtagtgtat tcatattgtg acaaatcttt agctatgtgc 53520

ctttcccaat cagaactcca gtctaatttt gatatatcca ataccctaac ggagttatcc 53580

ttgacttcta aaacagttct ggcaggattt tttgcaccta tatcaaaggc gcatattatt 53640

tcactcgaat aatctttttt tgacatcggt gacgatatta aagattgaga cgaactggtt 53700

aaagtttcca tatatagtct caatactcgg gttatttaaa caattagtat tttcaatatt 53760

gaaatttaca accttacaat tttctatttt gaaccgcatg ttattaacta acgttataaa 53820

atccgcttct ggatatccca aacattctct gcttaaatca aatgctgatt ggttttttat 53880

ttttcgtttt acagttccgg atttattaat ctcatcaact aactcatcga cagttgcgca 53940

ctcattaata tctaatttat tagtcaattg ctttaagcct gcactaccaa ccttagagaa 54000

atactttccg ttaacataat agtctcgtcc tatagtgtct ataattcgtt ttattacttc 54060

tcctctagtt tctgttccgt ttccatcata gtcgtataaa atggaaaatg tgtttttctt 54120

ttttactttt acaaatgtat gttctctaac cttggatcgt attaaatggt ctacatcttt 54180

tactaatatc ttttttcctg attttgttat aacgctagga ataaaaatat tatctcctat 54240

acgatctacc ttgatggact caatatacat gaatgagaat ttgaaaaagt ctacaacttg 54300

ccgcctaagt tctcccaact taatatacga tatggatatt tttggaaatt tatcatcgaa 54360

agagcgttct ataatagagt ataactcgtc gtcaaataat gtgtcttcct ctatttcaat 54420

ttctaaatat ttgggtatgt ttaatccgta ctcactagct agtttaaata tattaactgg 54480

agactgatat tttgatatag catcttccat tatccatcta ttgtttaaca aaacaaatct 54540

cagaaagtct attccgttat cgctgtctaa tactttgtta cacgcacttc gcatatcatc 54600

tggtatttga aaagagtttc cagatccaag ttctaccata tttttaccct tcgaataaat 54660

aaagacgctc ccaaaatata ccggtaagaa aatgtaatag cccggatcaa tattaaacgg 54720

tttatttttt acctcatcat aataatcaga tattgacgtc tcgacattag ctaccttttg 54780

cacgcctatt ttccagtaag tagatgttcc ccattctacc aaagaattat acttttctat 54840

agccgcagaa tctgaaaatt tcaaactttt gtacagacta agtaattctt ttaagttagt 54900

taaatcagcg ctagaagtca tgataacttt atttttaatc ctatgttatt tcattcttat 54960

ttttaatatt atagtacctg caatatctga aaaaatgaga cgcgaacgag ccgcatacgt 55020

aaactacaaa cgtctaaaca agaatttcat ttgtgtcgat gatagactgt ttagttataa 55080

ttttacaaca tctggaatta aggcaaaggt ggccgttgat aacaaaaatg ttcccattcc 55140

atgttccaag ataaacgagg tcaataataa taaagatgtc gatacactat attgtgataa 55200

agatagagac gatataccag gttttgcacg atcgtgctat agggcatatt ctgacttatt 55260

ttttactacc taaatggata gcaccaatgt gcgttccgga atgaagagcc gcaaaaagaa 55320

gcccaagact acagttatcg atgacgatga tgattgcatg acgtgttctg cctgtcagtc 55380

taaattggtt aagatttccg acatcacaaa agtatcattg gattatatta atactatgag 55440

gggtaataca ctggcctgcg cagcatgcgg atcgtcgctt aaacttctta acgattttgc 55500

gagttaaata ttctatttaa tacacgttct tctctggagg atgttgtaca tacagaagtg 55560

ggatcggatt cttgatgttt ccgataactt ttttgtttaa atcctagttt atctacagac 55620

agactaataa ttcgttgaac gaaatttcct atcatgtact ttatttcttt gatggatgtg 55680

ctaggaaata catataccgt cctatctaat agttctgttt ctcgacatac cctccctagt 55740

aattgctcta tttgcatatt gttgattact gccgagcaaa tgaacaaaga atccaaacta 55800

ggaatatcta aaccagtacc ggaataaaat aaggtggata cgaatataaa tctatttagt 55860

tccttgattg atttgaccat atctggagta cgtctatttt gggcgtctcc tataaataca 55920

acctctgatc cgaaaaaatc taataatcgt ttgtagaaga ataccatatg ttcacgtagt 55980

ttagtaataa ctaaaatgcg attaatagtt cctgacttga attcttctac cagggtatta 56040

agaataagtt gatttctagg ctcgtctaca gataataact tctcggtata tatatgatat 56100

ttattagatg gtccatctaa tcgttttatc atatgtctaa tattatctgt ggaatatggc 56160

tcaaaaaaac tatctactgc atagatagtt ttttttagat cggataactt ggcaatatta 56220

ataatactgt tacaataaat tctgttagat ggtctaggtg tagcagttaa aaaataacac 56280

atcatcggag gataatacgc taaaaatctt gtaactgctg tattgttcat cagattatac 56340

gtatgtgatt catccaagat gaacaaatca taatgcttat tgatatattt acaaaaggca 56400

tcgtttgtca gatgtctact gactactatt aatacatccg gactttgagt ctttagttcc 56460

tttaatagac tacttactcc atctatggat atcttatgtt ccaatccgac tgcctctacc 56520

tgtgtcttcc attgatgtat taacatttta ttgggtacgc aaatgacggt ttttctaccg 56580

tgtgtagcca taagataaca cgtggtaata gtcttaccaa atccacacgc caagtgaaga 56640

gtgatgtata gaggacgttt tgattcgatc attttcctca tattagaaac tacttcagat 56700

acgactttgc gttgaagagg atataacttt ggcgcatttg ttggattgat actagtaatt 56760

ttattatcgg tcgttgttaa tgtttcaaat actagactcg tctttacgga aggagaggcg 56820

taaaatccct taggtatcag aaacttaaag gatgatcccg gttcaacttc tacgaaatcc 56880

ccgtcttcgt taaaaagact taggggttga ccacaagtca ttttttttag ttcggcataa 56940

agattatact ccatctttaa tagtgacatt ttttaatata taaatgagtt atttaagata 57000

ttacaatatg cttgacgact tctctgcggg tgctggagtg cttgataaag atttatttac 57060

agaggaacag cagcaatcgt ttatgcctaa agatggaggt atgatgcaaa acgattatgg 57120

aggaatgaat gattatttgg gaatcttcaa aaataatgat gttagaacgt tactcggttt 57180

gattttgttc gtcttggctc tatatagccc tcctctaatc tctatattga tgatatttat 57240

ctcatctttt ctattgcctc ttactagctt agttattacc tattgcttag taactcaaat 57300

gtatcgtgga ggtaatggca acactgtggg aatgtctatt gtatgtattg tagctgctgt 57360

aattattatg gcaatcaatg tatttacgaa ttcacagata tttaatatta tttcttacat 57420

tattttgttt attctgttct ttgcatatgt gatgaacatc gaaagacagg actatagaaa 57480

aagtataaat gtaaccattc ctgaacagta tacctgcaac aaaccttata ctgcgggaaa 57540

taaggtaaat gttgatatac caacatttaa cagtttaaat actgacgatt attaaaaatg 57600

ggggcagccg ttactcttaa tagaatcaaa atagcaccag gaatagcaga tatacgagac 57660

aaatatatgg aattaggttt taattatcct gaatataata gagctgttaa gtttgcagaa 57720

gaaagttata cgtactatta tgaaacatct ccgggagaaa ttaaacccaa gttttgtttg 57780

atagatggta tgtcgataga tcattgtagt agttttatag ttcctgaatt tgctaaacaa 57840

tatgtattaa ttcatggaga accatgtagt tctttcaaat ttcgtcctgg atcattaatc 57900

tattatcaga acgaggtaac tcctgaatat attaaggatt taaaacacgc tactgattat 57960

atagcatccg ggcaacgatg tcattttata aaaaaggatt atctcctggg cgatagtgat 58020

agcgtggcaa aatgttgttc taagacaaat accaaacact gtccaaaaat atttaataat 58080

aattacaaga cagaacattg tgatgatttc atgactggat tttgtagaaa cgatcctgga 58140

aaccccaatt gtttagaatg gttacgtgca aaacggaaac cggctatgtc tacttattcc 58200

gatatttgct ccaaacatat ggatgcgaga tattgttctg agtttattag aattattcgt 58260

cctgattatt ttacttttgg ggatacggca ttatacgtct tttgtaacga tcataaagga 58320

aatagaaatt gttggtgcgc gaattatcca aaatctaatt ccggagataa atatttagga 58380

cctagggtat gttggttaca tgagtgcacc gacgaatcta gagatagaaa atggttatat 58440

tataatcaag atgttcaaag aactagatgt aaatacgttg ggtgcacgat taacgttaac 58500

tctttagcgt taaaaaattc ccaagcggaa cttacgtcta attgtactag aactacgtcc 58560

gccgttggtg acgtacatcc aggagaacct gtagtaaaag ataaaataaa actgcctacc 58620

tggttgggcg cggccataac attggttgta atatctgtta ttttctattt tatatctatt 58680

tattcgcgta ctaaaattaa aacaaatgat ataaatgttc gtagacgata attcgttgat 58740

aatttattct acatggccca gtacattgtc cgattcatcg ggtagagtca tcgttatgcc 58800

agataataga tcattcacgt ttaaggaagg gtttaaatta gatgaatcga taaaatctat 58860

attgttggta aacccgtcgt ctatagatct attaaagatt agagtatata aacatcgcat 58920

aaaatggatg ggtgatatat tcgtattatt tgagcaagaa aatatcccac caccttttcg 58980

tctagtaaat gataagtaat tacgagccgt tgctgctgtt agttataaca tgctgtgtac 59040

tactatttaa ttttaccata tcttcgaaaa caaaaataga tattattttt gcagtacaaa 59100

ctattgtttt tatatggttt atattccact ttgttcattc ggcgatttaa aatttttatt 59160

agttaaatgg atatgatgct tatgattgga aattattttt ccggcgtgct aatcgctgga 59220

atcattcttt tgattctttc gtgtatcttc gcctttattg actttagtaa gtctaccagt 59280

cccactcgta catggaaagt attgagtatt atggcgttta tacttggtat tattatcaca 59340

gtcggaatgc taatttattc tatgtgggga aagcactgcg cgccccacag agttagcgga 59400

gtcattcata ccaatcatag cgatatttcc atgaactaaa ttattatcgt ccatatatct 59460

cgacattgtt gaatcattat tactacttat ttagtgaaaa gatgatatat tgcatacttg 59520

atcaatagtg aagttattgt caataaatga ttggtattct tttgttgatc ggtatttgcg 59580

tagcagttac cgtcgccatc ctatatgcgc tgtataataa gatcaagaac ccacaaaatc 59640

caaatccaag tccgaattta aattcgcctc ctccagaacc aaaaaatacc aagtttgtaa 59700

ataatctgga aaaggatcat attagttcat tgtataatct agttaaatct tctgcataaa 59760

taaaaatatt tttagcttct aaatggcgga taaaaaaaat ttagccgtta gaagcagtta 59820

cgatgattat atcgaaacag ttaataagat tacaccacag cttaaaaatc tactagcgca 59880

aatcggtgga gatgcagccg tcaaaggagg caacaataat cttaattctc aaacagatgt 59940

gactgccggc gcatgtgata caaaatgtat tacatgtaaa ccaaaatcaa aatcctcgtc 60000

ttcttctaca tcaacatcca agggctccaa aaatacttct ggtgctccta gacgtagaac 60060

aacagttact actacatcgt acaatgcgat ggatggtcag attgtccaag ctgttactaa 60120

tgctggtaaa atagtttatg gtaccgtcag agacggccaa ttagaagttc gtggaatggt 60180

cggagagatc aatcacgatc ttctaggtat cgactcagtt aatgctggga aaaagaaacc 60240

atctaaaaag atgcctacta ataaaaagat taatatgtcg tccggtatga gacgacagga 60300

acagattaat ccagacgatt gttgtctgga tatgggaatg tattaaatta aataatttta 60360

attcgtttaa cgaatatctt gagtataaac aatacaatat taagaaccgg actgttaccg 60420

attcctagtt ttgatgctac agcatcgcag tcatctccga tctccacatc gataatttct 60480

gaagtgatat cggtactcag tcctttgatt tcttcaaatc cgagttttag caatgcttgc 60540

tcgataatat ttatgacaat aataatcacg ttagtgacga taatttttcg ttttctactt 60600

tcgtgatatt gtttaatcgt tttatagatt ctatctattt catcctctga acaaacatcc 60660

aagtcctcga ctgataaggg agacccgttc aaatttgcat aattaacgat ggctatacgc 60720

ttattaactt ctgttgattt atacggagtc ttaccaaata gtattctggt aaaattatct 60780

aaattatcag atgattttgt attagatcgt tgatttctaa gattaaccac ttcatcctcc 60840

aacatctgaa ttcttttatc tttatcataa actactccca aggatggtgt ttgtgttggc 60900

atagaagata tcatagatct agattccatt attgcttcag catttttcga tactgtaata 60960

ttaaatcttt tttctcgttg ttgacgttgt tccgatatca ataactctct acgaaacgta 61020

ggaggtggag tatcgtcaat aatagtagta tgaggttgta ctcgttcgat aatagtggcc 61080

ggagaatcat catctggaaa tagatcttca ttaagttgtg aggatatttc ctctggagat 61140

gacactacgg cggcattatg ttctctatct accacgttgt taacgtgagt tagaatagac 61200

atttgacgaa gagttatttc ataatttttg ttagatggat aattatcttc tgaaaactct 61260

gtaattaaat cgttttgtat atccgtcact ggtacggtcg tcatttaata ctaaataaat 61320

gatgcctatt aagtcaatag ttactcttga tcaattagag gactctgaat atttatttcg 61380

tatagtttct accgttcttc cgcatctatg tctagattac aaagtatgtg accaacttaa 61440

aacaaccttc gttcatccgt tcgatatatt gcttaataac tcattaggat ccgtaactaa 61500

acaagatgag cttcaggctg ctatatccaa attgggcatt aattatttaa ttgataccac 61560

gtcacgtgaa ttaaaactgt ttaatgttac acttaacgct ggaaatatag atattattaa 61620

taccccaatt aacattagtt cggaaactaa tcctatcatt aatactcaca gcttttacga 61680

tcttccacct ttcactcaac accttcttaa tattagattg acggatacag aatacagagc 61740

tagatttatc ggtggttata ttaaaccaga tggctccgac tcaatggatg ttctagcaga 61800

aaagaaatat ccagatctta actttgataa cacttatttg tttaacatcc tctataagga 61860

tgttattaat gcaccaataa aagaattcaa ggcaaaaatt gttaacggtg tattaagcag 61920

acaagatttt gataatctta taggtgttag acaatatata acagcacaag atcgaccccg 61980

ctttgacaac gcttataaca tcgcagatgc tgctagacat tatggagtta atcttaatac 62040

attgccatta ccaaacgtag atctcactac tatgccaaca tataaacatc tcatcatgtt 62100

tgaacagtac ttcatttata catatgacag agtggatatt tattacaatg gtaacaaaat 62160

gctcttagat gatgagatta tgaacttttg tatttctatg cgatatcaat ctcttattcc 62220

tagactggta gaattctttc cagatatacc agtaaacaat aacatcgtac ttcatactcg 62280

cgatcctcaa aatgctgcag tgaatgtaac cgtggcgctt ccaaacgtgc aatttgtaga 62340

cataggtaga aaccacaaat tctttattaa tttctttaac ctgttggcga aggaacaaag 62400

atctacggct atcaaagtta ccaaatccat gttttgggac ggtatggatt acgaggaata 62460

caagtctaaa aaccttcagg acatgatgtt tataaattct acctgttatg tattcggtct 62520

ttataatcac aataatacta cttattgctc tatcctttct gatattatct ccgcagagaa 62580

aacacctatt agagtttgtt tgttacctag agtagtcgga ggtaagactg ttactaatct 62640

tatttcagaa actttgaaga gtatttcatc tatgactata cgagagtttc ccaggaaaga 62700

taaatctatc atgcatatag gactttctga gacaggattc atgagattct tccaactact 62760

caggctcatg gctgataaac ctcatgaaac ggctattaaa gaggttgtta tggcttatgt 62820

gggtataaag ttgggtgaca aaggtagtcc gtactatatt agaaaggagt cataccaaga 62880

ctttatctat ctgctatttg catcaatggg ctttaaggtg actactagaa gatccattat 62940

gggaagtaat aatatctcta tcatcagtat tagaccaaga gtaactaaac aatacatcgt 63000

cactacattg atgaaaacta gttgtagtaa aaacgaggca gaaaaattaa ttacttcagc 63060

gtttgatctt ctcaatttca tggtatcagt tagtgacttt agagattatc agagttacag 63120

acagtataga aactattgtc ctagatattt ctatgcagga tctcccgaag gagaggaaac 63180

cattatctgt gactcggaac cgataagtat cttggataga attgatactc gtggtatctt 63240

ttctgcgtat actattaatg aaatgatgga cactgatatc ttttctccag agaataaggc 63300

atttaagaat aatctgagta gatttatcga gagtggaaat attacaggag aagatatttt 63360

ctgcgcaatg ccatacaaca tcttagatag gattattaca aatgctggta cgtgtaccgt 63420

atccataggt gatatgttgg ataacattac aacccagtca gactgtaata tgactaacga 63480

aatcacagat atgataaacg cctcattgaa gaatacaatt tctaaagata ataatatgct 63540

agtcagccaa gcattggact ctgtagctaa tcgttctaaa caaacgattg gagacttgag 63600

gcaatcatcg tgtaaaatgg cattgttgtt taaaaatctt gctacatcca tctacacaat 63660

agaacgtatt ttcaatgcta aagtaggcga tgatgttaag gcatcgatgt tggagaagta 63720

taaagtattc acagatattt ccatgtcatt gtataaagac ttgatagcta tggagaatct 63780

caaagcgatg ctatacatta ttcgacgaag cggatgcaga atagacgatg cacaaattac 63840

tactgacgat ctagtcaagt cttactcatt gatccgtcct aaaattctaa gtatgataaa 63900

ctattataat gaaatgagta gaggatactt tgaacacatg aaaaaaaatc taaatatgac 63960

agatggtgac tctgtctctt ttgatgatga ataaatgtca tgttatacag ctatattaaa 64020

atctgtagga ggactggcgc tatttcaagt agccaatggc gccatagatt tatgtagaca 64080

tttctttatg tatttttgtg aacaaaagct acgaccaaat tcattttggt tcgtcgttgt 64140

tagagccatt gcaagcatga taatgtattt agtattaggc atagcattgc tgtatatttc 64200

tgaacaagat aacaagaaga atactaataa tgataaacga aatgagtcgt ctataaattc 64260

taactccagt cctaagtaaa atattttagt agcgtatcct tatcgcgttt gcttatcttg 64320

aatgaactgg tcttttttaa ctcgttgatg aattttgtaa ataattcatt tctatcttta 64380

agtaatttta ttggaagttt tgattccaca gccaatgaac atgtaaattg tgaatcattt 64440

tctatgacca gttgtaactt ttcatacact gtaatcaata cagtatttac catttgatta 64500

attacagcgc ccgtactagc actccattga acaagatcag attttagatt aattagataa 64560

aatctatatg ttatttcaga taatgaactt accagataac tctcttgacg gatattaaaa 64620

atgccagatg aatgttcatc tcgaatagcc agagctactg tagagtaata cggttcaaaa 64680

tcataaacgt gatagtttcc aaactggtat ccgagttttt cttggatgat ggatacttga 64740

gagttgaatt taaatttctt actatgttca ttatatagtt ctggaaagaa tgcttcaatc 64800

attgtttctg taagtttgca tccttttgat gcgatagtta aaagtgctat ataaggcgcc 64860

acgataatgt tatttttttt ggtaatagtg acgtctttta cagcgtcaac gcatctgaac 64920

aaataatgaa tctctctatg atctaaaaaa taaagtcttt gtaaaaagaa tcgtagtgct 64980

agttttctct catcatcctt agatctatgg gcgaatagac gtctactacg ggatataaaa 65040

ccgctatttt cctttatcat aggtgttgtt tgatctatat ttacctcccc tagttctacg 65100

gaggcctcca aattaagatc tggtactggt tcgaacattg taagacttac atcatcggta 65160

gtagattttc actttacccc acgatataaa tatgcgatat atagtaagtc cgcaattggt 65220

attacaggtg ggtaaggggc aggaggtaga acgagcccta tatctcactc catatgatta 65280

catagatgag aagtcgccca tatattattt tttacgaagt catttaaata tacaacagcc 65340

ggaaatagtt aagagacata ttctattgac gcttcgaatg actcaattaa agggatattt 65400

aggaaatttg ttagatatta aggacgatat tattatctat tctcataaga ataatttgga 65460

atatagttac gttgataata ctatttttaa tcccttcgta tatactcaga aaaaaacact 65520

actaaaaaac gatagctttt tatacaatgt atatcctgga gcgtgtgact ttttggttat 65580

ctgggtggcc agagcgtgcg atacatctat tccggaattt ggatcgtatg aagatgtaga 65640

taataatatt atcaagtttg aaacaatgtt gatggaagta tttccacaac tagatttgga 65700

cattactgta gaatcaaagt ttaacaatat atttcgtacc aatctaaaac taactgggtt 65760

aaaaaagatc attcagcgag ttcaagactt ggacattaat tataagtcgt tgttatctag 65820

atacgatgaa cactttatta atatgaccgg taatcatttt attctaaacg atgaacagtt 65880

aaatctctcc atttgggact tggatggtac attagcgtta tctagcgacg gcgataccgt 65940

gatgattaat aacgtaaaac tatttacaga tcttgtgtcc gatatagata cacaaatgga 66000

acgcatcaag ggagatataa cgtataaggt acatttggcg actcctatca attctagaat 66060

aaaattggat atcgagacta gcttcatttt tatagagacg gcgactaata atattttact 66120

atcctcggat aaaaaaatat ctatcatttt ggccaaaaac catatatcta ttaaagtgaa 66180

aaaccatatt cctaacatag aaaaatattt tacattttta gttattgcca ttaatgccat 66240

gtttaatagc gttcaaaagt ctgctgattt taccaaagtg gaaactgttt actggtctag 66300

gatatgccaa aatacaaaga ataagaatag aaaacccatc attattaatt atctagatcc 66360

tggaatgaaa aaaatcagta acaactttta cagatccgat gagaaagaag tctttattaa 66420

tgataacggc ataatgttta catgcatgga tcctttgggg aaatataata aggtgggatt 66480

tcttaatata tttcatgata tgtggaaata ttgtatccct tgttgttttc tacatgatca 66540

atctcatcga agtacatttt catcgtgtgt tcatcaaatc gacgttgaga aaaagatagt 66600

aagtccgtat atccttaatt ttggtaaagt tgtaacagaa tccaaaatgt catttctccc 66660

tattatcttt gacgccttct taaatgatgg aatgactgct aatatggaac aagataataa 66720

acgactaaag gaaactagtg gatatcatat agttagatgt tgtgctggtg atgatatagt 66780

tcgtttacga actacatctg atattattca gtttgtaaac gaggataaaa atattcttat 66840

agttaacgac atggtatatt ttccaatgaa cgcgtccgat ataggaaaga aaatacatat 66900

actcattcaa gaaatagttc atgaggtaat gatagtaaaa aagaaagagt ctagtgataa 66960

aatcgatttt ttcccaccca actataagtt attgaaggat ctatttccaa aacaaactat 67020

tcaaactcct attcaatctg acgcgggaat ggtgttaaca accgatggat tctacataga 67080

tggaaaactt tttaacgaag atctgtcgtc taaatacgtt acatttacaa aaaatgttat 67140

tgcgtctgat gccgtagcta aatatttttc tcctttgttt aaatacgtta tttcagaagc 67200

taaagataga tttatcaaaa cgtggatgat taatattatg atacatatga acgtagatcc 67260

taataatata ataccgacgt tagaaaaata ctatcccaac tctggaagag cacaaataaa 67320

ttaaacaact aaatctgtaa ataaataatg gacaaactta gagttctata cgatgagttc 67380

gtcaccatta gtaaagataa tcttgaacgc gagactggtc ttagcgcatc agatgttgat 67440

atggattttg atttaaacat ttttatgacg ttggttccag tcttggagaa aaaggtatgc 67500

gctattacac caactataga agatgataaa atcgtaacta tgatgaaata ttgtagttat 67560

cagagttttt cattctggtt ccttaaatct ggtgccgttg tgaaatcggt atataataaa 67620

ctagatgatg tggaaaagga aaagtttgca gccacattta gagacatgtt gcttaatgta 67680

caaactctaa tttctcttaa ctctatgtat actagattgc gtcaagatac cgaagatatt 67740

gtatccgatt ccaaaaaaat aatggagatt gtttcccatt tgagagcgtc gactacagag 67800

aacgcggcgt atcaagttct ccaacaaaac aatagtttta tcatatctac actaaataaa 67860

atcttatctg atgaaaacta tctcttgaaa attattgcag tattcgactc taaactaatt 67920

tctgaaaaag agacattgaa tgaatacaaa caattgtaca ccatttcttc tgaaagtttg 67980

gtatatggaa tcagatgcgt tagtaatctg gatatatcat ctgttcaact gagtaacaat 68040

aaatacgttc tctttgttaa gaaaatgcta cctaaaatca tactgtttca gaataacgac 68100

atcaatgcac aacaattcgc taatgttatt tctaaaattt atacgttgat ttatagacaa 68160

ttgacgtcga atgtcgatgt tggatgtcta ttgacagata cgatagaatc tgccaaaact 68220

aaaatatctg tagaaaaaat taaacagacg ggtatcaata atgttcaaag tcttatcaaa 68280

ttcatatctg ataacaagaa agaatataag acaataatct ctgaagaata tctatcgaag 68340

gaagatagaa tcattactat tttgcaagat atcgttaatg aacacgatat aaagtacgac 68400

aataaattgc tgaacatgcg agacttgatt gtgacattta gagaacgata ttcgtataaa 68460

ttctaatatc gtttttgaag tttccaaatt ttgataatat agtctagatg gaattttaga 68520

ccatctttgt caaaatcgtt taccgacaac aattctccgt tcttttcgat gactatagga 68580

caagaaccct cctctatctc ttgtataatt acatgtagca tattttgctt catcgtttcg 68640

tcagaaaatt ctgaaactag aggtaatctt cctcgttgaa gcaagttata gctttctgct 68700

attatacccg ctatttcaaa tagacttata cgtctagtat agcgtcgttt aagagcagat 68760

atatgattcc ctatatgttt aagattggaa tgcgcatctt ctatatgagt ggatgctgat 68820

tctacaatct tataagaaga tttgggatct atatcactag tttctagtga ctctccatct 68880

tcttcctctt cttcatcatc ctcgtattcg gtgagatcat cggattcata gtcgataata 68940

tcgtctgtgt ctgccattta atcttataat cgcaattcaa ttttaaagcc ttaaatggac 69000

ttctttaaca agttctcaca ggggctggca gaatcctcta caccaaagtc gtcaatctat 69060

tattctgaag aaaaggatcc ggatacgaaa aaggatgaag cgattgaaat aggactaaag 69120

tctcaagagt cgtattatca aagacagttg cgagaacaac tagctagaga taatatgatg 69180

gccgccagca gacagcctat ccaaccgcta caaccaacta ttcatataac tccacagccg 69240

gttccaacag ctacaccggc tcctacacca aaaccacgac aacaaactaa tacatcatct 69300

gatatgtcta atctttttga ttggctgtct gcagatactg atgcgccggc gagttcactc 69360

cttccagcgt tgacgccgag caatgctgtt caggatatta tctctaaatt taataaagat 69420

caaaagacga cgacgccgcc atctacccaa ccttctcaga cgttaccaac aactacatgt 69480

acacaacaat cggatggaag tatttcttgt actactccaa cggttacacc tcctcaacct 69540

cctattgtgg ccactgtatg tactcctaca cctactggtg gtacagtatg tacaacagca 69600

caacaaaatc caaatccagg agcagcatct caacaaaatc tagacgatat ggcccttaag 69660

gatctcatgt cgaatgttga aagagatatg caccaacttc aggccgaaac aaacgatctg 69720

gtgacgaacg tatatgatgc aagggagtat acgcgtaggg caatagatca aattctacaa 69780

ttagtcaaag gttttgaacg attccaaaag taataagatt gaatattaaa atcacgcttt 69840

cgagtaaaaa ctacgaatat aaataatgga agccgtggtc aatagcgatg tttttttaac 69900

atctaacgca ggactaaaat ctagttatac taatcaaact ctttctttgg tagatgaaga 69960

tcatattcac acttctgata aatctttgtc ttgtagtgta tgcaattcat tatccaaaat 70020

tgtagacgat gactttatat ccgcaggggc tagaaatcaa cgtaccaaac ctaaacgtgc 70080

aggaaataat caatctcaac agcctatcaa aaaggattgt atggtttcca tcgacgaagt 70140

agcatccacg catgattgga gtacgagatt gagaaatgat gggaatgcaa ttgctaaata 70200

tctaactact aacaagtatg acacatctaa ctttactatt caggatatgc ttaacattat 70260

gaataaacta aatattgtca gaacaaatag aaacgagcta tttcaactcc ttacccatgt 70320

aaagagcaca ttgaacaatg ctagtgtttc tgtgaaatgt actcatcctt tagtacttat 70380

tcattctcga gctagtccta gaatcggtga ccaactcaaa gagttagata aaatatactc 70440

tccatctaat catcatattc ttctgtcgac tacacgattc caatccatgc attttaccga 70500

tatgtctagt tcacaagatt tgtcttttat ttatagaaaa ccagaaacta attactatat 70560

tcatcctatt ctgatggcac tattcggtat taaacttcct gcgctcgaga acgcgtatgt 70620

acatggagac acctatagcc taatccagca actttatgaa tttagaaaag taaagtctta 70680

taattatatg ttgttggtta atcgtcttac ggaggataat ccgatagtga ttacaggtgt 70740

atcagatcta atttccacag agattcagag agcaaacatg cataccatga ttagaaaagc 70800

aattatgaac attagaatgg gaatttttta ttgtaacgat gatgatgcgg tagatcccca 70860

tctaatgaag attattcata ctggatgctc tcaagttatg acagatgaag aacagatatt 70920

ggcttctatt ttgtctatag ttggatttag acctacgttg gtttctgtgg ctagacctat 70980

aaacggcatc agttacgata tgaaacttca ggcggcacca tacatagttg ttaatcctat 71040

gaagatgatc acaacatccg acagtccgat ttctatcaat tccaaggata tttattctat 71100

ggcattcgat ggcaatagtg gaagagtggt gttcgctcct cctaacatag gatatggaag 71160

atgttctgga gttacacaca ttgatccatt gggaactaat gtgatgggta gtgctgttca 71220

ttcccctgtt atcgttaatg gagcaatgat gttttatgta gaacgacgtc agaataagaa 71280

tatgtttggt ggagaatgtt acaccggctt tagatctcta atagatgata ctccgattga 71340

cgtatcacca gaaatcatgc taaacggtat catgtatagg ttaaagtccg cagtttgtta 71400

caaactcgga gaccaattct ttgattgtgg atcgtctgat atcttcttga agggacatta 71460

tacgattcta tttacagaaa atggaccctg gatgtacgat cctctttctg ttttcaatcc 71520

gggagctaga aatgctagat tgatgcgagc tctcaaaaac cagtacaaga aattatcaat 71580

ggattcagac gatggttttt atgaatggtt gaatggcgac ggttcagtat ttgctgcctc 71640

aaaacagcaa atgttgatga atcacgttgc taactttgac gacgatcttc taactatgga 71700

agaagccatg tcgatgattt cgagacattg ttgtatctta atttatgcac aggattatga 71760

tcaatatatt agcgctagac atattacaga actattttag attatgatat ttaaatgagt 71820

tggtacgaaa aatataacat tgtactgaat ccgcctaagc ggtgttcttc tgcatgtgcg 71880

gataatttaa ctactatatt ggcggaggac ggtaaccata ttagggcgat actttattca 71940

cagcccaaaa aactaaaaat attacaggat tttctggcaa cgtctagaaa taaaatgttt 72000

ttatataaaa tattggacga cgagatacgt agagtgttaa catgaatcta cgattatgta 72060

gcggttgtag acacaacggt atagtatctg aacaaggata tgaatattgt attttttgcg 72120

agtctgtatt tcaaaaatgt acaaaggtac aaaaaaagtc aaacttccat gtgtctaata 72180

aacttattca tttgagaaat gtattgcgga gattattgtc tcatcaatgt tctggagaaa 72240

ttatctcgga actcttggat atcatggaaa aaaatcaaat atccacggat gatgtagatg 72300

caaattttgt atctagtttt cttaaggcta acgagagaat aaataaaaag gattataagt 72360

tagtctttga aataatcaat caagtaaaag atgagaaact gaatctgagt acagaaaaga 72420

ttaatgaagt agtagaaata tttaagcact tggtattctt ttgccaagaa aacactcctt 72480

ctaagacaat taattattca ttctttttgg ataaaatatt cgatatcact tctgtaacta 72540

aaaatctaaa acctcaaact gttaaaaatt atacgaaaaa taatagtaac caattagtat 72600

gggaaaactt tttagcacat atgagatcta aaaaacgtgt aactatggta gaggattatg 72660

gacacgagta tgtttttgta gatgagaggt tttctacttg ctcattagaa gtataaaaaa 72720

atagttccgt aattaaatgg ctaagcgagt aagccttcca gatgtggtta tttcagcacc 72780

taaagcagtc tttaagcccg ctaaagaaga agcactcgct tgtatactac caaagtatta 72840

taaatctatg gcagatgtgt ctattaagac aaatagtgta attgataagt gttggttttg 72900

taatcaagat ttggttttta gacctattag tattgagaca ttcaagggtg gtgaagttgg 72960

gtatttctgt tctaaaatat gtagggattc gttggcttct atggttaagt ctcacgtagc 73020

tcttagagaa gaaccaaaaa tttctttgtt gcctttagta ttctatgaag ataaggaaaa 73080

ggtcataaat acaataaacc tactaagaga taaagacggc gtttacggaa gctgttactt 73140

taaggaaaac tcacaaatta tagatatttc tctacggagt ttattgtaag ctttttccat 73200

tttaaataga aaatgaataa tactatcatt aattctttga tcggtgggga tgactctatt 73260

aaacggtcta atgtcttcgc agtcgatagt caaattccaa ctttgtatat gccgcaatat 73320

atttctctat ccggagttat gacaaacgat ggtccagaca atcaggctat cgctagcttc 73380

gaaattaggg atcagtatat tactgcgctt aatcatttgg ttctgagttt ggaacttcca 73440

gaagttaaag gtatgggaag attcggttac gtaccatatg ttggatataa atgtattaat 73500

cacgtatcta tctcttcgtg taacggtgtt atttgggaaa ttgagggcga agaattatat 73560

aataattgta tcaataatac aattgctttg aaacactctg gatattctag tgaacttaat 73620

gatatttcta ttggcctaac tcctaatgac actattaaag aaccatctac agtatacgtt 73680

tatattaaaa ctccgtttga tgtggaagat acattcagca gtcttaaact atccgattca 73740

aaaattaccg taacggtaac cttcaatcca gtatccgata tcgttattcg tgactcttcg 73800

ttcgactttg aaacgttcaa caaagaattt gtttatgttc ctgaattgag ctttattgga 73860

tatatggtta agaatgtaca aattaaacca tcgtttatag agaaacctag gagagtaata 73920

ggtcaaataa accaaccaac ggcgactgta actgaagttc atgcggcaac atcgctctct 73980

gtttatacta aaccttatta tggaaatacg gataataaat ttatttcgta tccagggtac 74040

tcacaagatg aaaaagatta tatagatgca tatgtgagta gattgttgga tgatctagtt 74100

attgttagtg atggcccacc gactggttat ccggagtctg ccgagattgt tgaggttcca 74160

gaagatggta tcgtttctat tcaagatgct gatgtgtatg taaaaattga taatgttcct 74220

gataatatga gtgtttatct tcatactaat ctgctaatgt ttggaacacg aaaaaattct 74280

tttatatata acatttctaa aaagttttcc gccattactg gaacatatag tgatgccact 74340

aagagaacaa tctttgctca catatcacat agtatcaaca tcatcgatac atctattcct 74400

gtaagtcttt ggactagtca acgtaacgtc tataacggag ataatagatc agccgaatca 74460

aaggccaagg atttgttcat taacgatccc ttcatcaagg gaatagattt taagaataag 74520

accgatatta tttctagact agaagttaga tttggaaatg atgttctata ttcagagaac 74580

ggacccatct cgagaattta taatgaacta ctgacaaaaa gcaataatgg aacaagaacc 74640

ctaactttta actttacacc aaagatattc tttaggccga caactattac ggccaatgta 74700

tctaggggga aagataaact atctgttcga gtagtttatt ccaccatgga tgtcaaccat 74760

ccaatctatt atgtacaaaa acaattggta gttgtatgta atgacctgta taaggtatct 74820

tacgatcaag gggtaagtat taccaagatt atgggagata ataactaata ataatgaaaa 74880

caaactatag agttgtaaat ggatgaaatt gtaaaaaata tccgggaggg aacgcatgtc 74940

cttcttccat tttatgaaac attgccagaa cttaacttgt ctttaggtaa aagcccatta 75000

cctagtctgg aatacggagc taattacttt cttcagattt ctagagttaa tgatctaaat 75060

agaatgccga ccgacatgtt aaaacttttt acacatgata tcatgttacc agaaagcgat 75120

ctagataaag tctatgaaat tttaaagatt aatagcgtaa agtattatgg gaggagtact 75180

aaagcggacg ccgtagttgc cgacctcagc gcacacaata aactgttcaa acgtgaacga 75240

gatgctatta aatctaataa tcatctcact gaaaacaatc tatacattag cgattataag 75300

atgttaacct tcgacgtgtt tcgaccatta tttgattttg taaacgaaaa atattgtatt 75360

attaaacttc caactttatt cggtagaggt gtaatcgata ctatgagaat atattgtagt 75420

ctctttaaaa atgttagact gctaaaatgc gtaagcgata gctggttgaa agatagcgcc 75480

attatggtgg ctagtgatgt ttgtaaaaaa aatttggatt tatttatgtc tcatgttaag 75540

tccgtcacta agtcttcttc ttggaaggat gtgaacagtg ttcaatttag tattttaaac 75600

aatccagtgg atacggaatt cattaataag ttcttagagt tttcgaatag agtatacgaa 75660

gctctctatt acgttcactc gttgctttat tctagtatga cttctgattc aaaaagtatc 75720

gaaaacaaac atcagagaag actagttaaa ctactgctgt gatttttaaa acatagttat 75780

tacttatcac tcataaatga gtaaatcaca cgcggcctat atcgattatg cattgcgcag 75840

aactactaat atgcctgttg aaatgatggg aacagacgta gtacgcctca aggattatca 75900

acattttgta gcaagagttt tcttaggatt agacagtatg cattctcttt tattgttcca 75960

tgaaacgggt gttggtaaaa caatgactac tgtatatatt ctcaaacatc ttaaggatat 76020

ttatacgaat tgggctatta tcttattggt gaaaaaggct ttgatagaag atccttggat 76080

gaacactata ctcagatacg ctccagagat aacgaaggat tgtattttta ttaattacga 76140

tgatcaaaat tttagaaata aattttttac taatatcaaa actattaatt ccaagagtag 76200

aatatgcgtc attattgatg aatgtcataa cttcatttct aaatcattaa tcaaagaaga 76260

tggtaagatc cgtcctactc gttcagtata taatttttta tctaagacca tcgcattaaa 76320

aaaccataag atgatttgtt tatcggctac acctatcgtt aatagtgtgc aagaattcac 76380

catgttggtt aacttactac gaccaggatc cttacaacac caatcgctat ttgagaataa 76440

acgtctagtt gatgagaaag aattagtctc caaactagga ggcctatgtt cgtacatagt 76500

taataacgag ttttctattt ttgatgacgt agaagggtct gcatcattcg ctaagaaaac 76560

agtattaatg cgatacgtta atatgtcgaa aaagcaagaa gaaatttatc aaaaggctaa 76620

actcgctgaa ataaaaacag gtatatcatc atttagaatt ctgagacgta tggctactac 76680

gtttacgttc gatagctttc ctgaaagaca aaatcgtgat ccgggcgaat acgcgcaaga 76740

gatagcaaca ctatataatg attttaaaaa ttcattaaga gatagagagt tttctaaatc 76800

cgcattagat acctttaaaa agggagaact attgaaaggg gatgctagtg cggctgatat 76860

ctctctattt actgaattaa aagagaaaag cgtcaaattt atagatgtat gtttgggaat 76920

attagcatcc catggtaaat gtctagtctt tgaaccattt gttaatcagt caggaataga 76980

aatcttatta ctatatttca aagtctttgg tatctctaat atagagttct catctagaac 77040

aaaagatact agaatcaagg cggtggctga gtttaaccaa gaatcaaaca ctaacggaga 77100

atgcattaaa acatgcgtat tctcttctag tggaggcgag ggtattagtt ttttctcaat 77160

taatgatatc ttcattttag atatgacatg gaacgaggcg tctcttcgtc agatagtagg 77220

aagagccatt cgtctcaata gtcacgttct tactcctcca gaacgtagat atgtaaacgt 77280

gcactttata atggctagat tatctaatgg tatgcctact gtagacgaag acctatttga 77340

aatcattcaa agcaaatcaa aagaatttgt ccaattgttt agagtgttta aacatacatc 77400

attagaatgg attcatgcta atgaaaaaga cttctcaccg atcgacaatg agtccggttg 77460

gaaaaccttg gtttcaagag ccatcgatct atcgtctaaa aaaaatatta ccaataaact 77520

aattgagggt actaatattt ggtattccaa ttctaataga ttaatgtcaa taaatagagg 77580

atttaaaggc gtagatggtc gagtatacga tgtagacggt aactatctac atgatatgcc 77640

ggacaatccc gttataaaaa tacacgatgg taaattaatt tatattttct aatcaatcat 77700

cctcagttaa tttttttaat gattcgtaat aacatcctct atggccataa catttgagtt 77760

ttgcagtatc tagagcatat tttgcaattt cgtattggag tccattacct gaattcggat 77820

ctaaaaatat tagatccttg atttcatgat tacttttaaa tgtatcaatg atttgatcac 77880

tcgttaaaga tattcttccg acaaagaaga ttacctcaaa aaatttatta ataatggtat 77940

cttctatgat gccatgaata aaaaaccgag tgtctatgaa tacaaaagaa ttgtctatat 78000

taacttcttc tttaatttcc ctggataaac actctggaac attctcaccc cttttgggta 78060

tgccacccgg ataaatagca tctattctat cattatcagt agtagctgga tctagagaaa 78120

aaaatgacga tagtatactt ctttcctgtt tggacaaata attggaataa ttcagaaata 78180

atcgtttctt tctagacatg tttctagttc taattatttc agaatagaga aaactatctc 78240

gtctgttaca tactaatatt ttgttatcag tagttaataa aatagcggac acggatactc 78300

gtttatgaca ccataggcat tggttaacga atgccgtgag tgtaataatt tgagagtcat 78360

cctcgcaaat aatagactta gctagtcgtc tattatactt aataatctga ctaattatac 78420

tagatctgta aaagttcatt tactattaac tagcatatta taaatataag acagatattc 78480

gtatttatcg tcgttaatac aatcatttaa tgatttaatc tttctaattt ctacattgta 78540

aatactagta tgcgataaat cctccaacga ttgattaata aaacacgcca tgcaataact 78600

tatatatact ttattaaata atttatcccg tgttgtaagt tttagaatta catttccaaa 78660

ttcttttact gttatacgtt catcactttc ctcttttaat tctcttctta aacaatcttt 78720

aatagattcc tttttatcta gtttaccacc caataatatt aactcttcaa agtgaggatc 78780

gatatcgttg atagaacctt ttctcaaccg tctaaagatt tctcttagtt cattgtagta 78840

cataaaccgt aatagtttag tggatactct aaagatagaa tctgaatttt gttgagataa 78900

tatcgcttgg aatgcgaatg aagttcttct agctcctatt aacggatatc cgtcacttgt 78960

tatacacgca gcaaacacat gcgtgtcttt tgatcttgga atatctttta ttcgtttaat 79020

agatattaat tctctaggag tttcaaatat cacttcctca tccattgtaa ttcccatact 79080

aagagctatt tttaaacagt tatcatttca tttttactat gccgcaacaa ctatctccta 79140

ttaatataga aactaaaaaa gcaatttcta acgcgcgatt gaagccgtta gacatacatt 79200

ataatgagtc gaaaccaacc actatccaga acactggaaa actagtaagg attaatttta 79260

aaggaggata tataagtgga gggtttctcc ccaatgaata tgtgttatca tcactacgta 79320

tatattgggg aaaggaagac gattatggat ccaatcactt gatagatgtg tacaaatact 79380

ctggagagat taatcttgtt cattggaata agaaaaaata tagttcttat gaagaggcaa 79440

aaaaacacga tgatggactt atcattattt ctatattctt acaagtatcg gatcataaaa 79500

atgtatattt tcaaaagata gttaatcaat tggattccat tagatccacc aatacgtctg 79560

caccgtttga ttcagtattt tatctagaca atttgctgcc tagtaagttg gattatttta 79620

catatctagg aacaactatc aaccactctg cagacgctgt atggataatt tttccaacgc 79680

caataaacat tcattctgat caactatcta aatttagaac actattgtcg tcgtctaatc 79740

atgatggaaa accgcattat ataacagaga actatagaaa tccgtataaa ttgaacgacg 79800

acacgcaagt atattattct ggggagatta tacgagcagc aactacctct ccagcgcgcg 79860

agaactattt tatgagatgg ttgtccgatt tgagagagac atgtttttca tattatcaaa 79920

aatatatcga agggaataaa acattcgcaa ttattgccat agtattcgtg tttatactta 79980

ccgctattct cttttttatg agtcaacgat attcgcgaga aaaacaaaac tagattcgat 80040

accttgttga gcctccatta gaacggcagt gacttcgctg ccattgtcat acgcattacc 80100

atttcgaaaa aagcagtact ttgaatcgct aaatgataca gtacccgaat ctctacttag 80160

tttacagatt aaatctccac attgaatagt tacatttgat tcatcttcga tgtttaatgt 80220

tcctctgact atatccccaa cgtgataata cgcgtaggtt attacacacg gaacgtttat 80280

aactacagaa ttattaacta tttcgccgag aggtaattcc acgtcttcac gaatttctat 80340

tttcttggcc attttaccac taatttctct atggagatac gtcttatata cggcattcct 80400

aatattagtt tttatgtcta acgtcagctc gtgtggctcc aatgtaactg gaaggtatcc 80460

attggtaaca aagctcgaca tttatttctt tatatatctc atcagtttta tggagaagat 80520

accacgttat attctttacg aaagttaact ccccaaacaa atggttcgta ggaaagtcta 80580

aacggtactg taataatatc gttttcgtat ttgtataaaa gagatttatc aaatacttta 80640

ttcgatacaa agaagtgacc gtttacaata tcatctatgt aattcctagc gtcttcttta 80700

ttttttataa ctgatgtaac cattttaagt aacttggtat cgttatactt aatacgagaa 80760

ttattataaa aaaattgtct gaccaattct cccaataaaa cttttacaat tgatggatgt 80820

ggtggaagag aatacgtttc agacatctct tgaagaatag agtatattct attcgtttct 80880

ttagtcttaa attttagata caacagcttt ttgatgtcaa atggtaaaac attaattaat 80940

tcatcctgtg tgtaatcgtt taatgacgtc acttcgtcat tgaaatcgga atatacggcg 81000

gctaaaagat atacattaac tggttcagaa atatcggcgt aagagaattt tctaatagat 81060

cgtccaagaa tttggttgta ttgagaaaaa gtatctggga tagtcataaa ccaaatatgc 81120

cttacctctt tcagagtata ggattcggac ataatgtttg acgaaaacaa aaacatcaat 81180

tgactgccat catcgttttc aggagaatta tacacatcta atagatcctc taaagacgat 81240

ttcattttac tagtaacgat agcaaatgtt tttggtttgc cgtttatcat atgtggatta 81300

gttccctgag aaccattata ttcagaatat ccattactga gcatgatata tttaattacc 81360

aatccgccat atgtagaatt agaaaagtat ataaaatgtt ttccgttgag tgtctgtatc 81420

cgattaataa agtatttaaa tttggaacta atgtttaacg ttaccaattc ttctccgtat 81480

aacacgccat tatttatttt cagatttggg tacaattcct tatcctgttc ctgaaataaa 81540

gtatctaaat tattcatcag attaagttgt cccaatactg ccattgacac gttatacata 81600

tttttatcaa acatttcatg ataacatagc tgtcgtctag taatcatata atctctctct 81660

tgaagtttag acatgtgaca atatactact ctagtatcta gaaacttacg tccgtgatat 81720

cttatcgttg gtagatcttt atcaggcatt tcgtaatatg atattcttcc tttaagcaaa 81780

tccttaagta cattcacacc tcgttcgtta agaagtgtct gaattacttt cttaccacga 81840

ctaataattt caccaaaatc tatcgtctct tcggacatta aatctataat atgacccaga 81900

gtattaggtg tgttagtaat gggagatcca gacaatagta aaaaaggaat cttgttttta 81960

ttttttatca cggtcataag ttctccagta ttattcccaa agatattatg tgcctcatca 82020

acgataaaaa tagagttatt gtagcgagat aatccgttat aattaatgac gttatcgtta 82080

taattaagag aataaaaact tgttgtggaa tgaataaaga tattctcagc tatgaattcg 82140

tcattaaaca agttcatagc tacacccata ttataattaa aaattttcaa aatgttaata 82200

ttaggcacta gaatgtaaac ctttttaaat ctggaagcta ccaaggcgaa caacaaagcg 82260

attattgttt tacctgatcc cataatatgg aacaataaca cgcttctgtt ctcatctatg 82320

atagttctaa ctagataatc tagagtagct aactgatgag gtaatatagt tggtatacta 82380

tcaacatgat tatcaaataa atctatgatt ccggtattca tttagatatg tgatatcttc 82440

tataaatata tgagcatata tttacggaga tgaaatatcc tctatgaata tatattgcag 82500

atattcatca ctaacattgg caaatttctt atgtctattg atgaagctct ctatatcgtg 82560

tccaaatagt ctagaattaa aatacttgga tattttctgt tggaaagtag tcaacggaag 82620

agtaaccaca ttatcgtaca atatatatcc ctttttggag aggtccgtca ttaatggaat 82680

atgctttaca gagctagata ctaacagagt acctattttg agatagaatg caaagtccgg 82740

aatctcttcc ggtgtaggat atagtttcat aataggaaca tgatattttt tgtaccattt 82800

caccaacaag tatagaaatg cgaatctata tctattattt tgtattttac catctaaccc 82860

ctcgtctaat agtttgactt tatcgtacgc gtcattattt tcagcagcct ctctaccaga 82920

aggttgagaa aagtgtgttc tgaatcgcac gacggcaatt cttctcatta atgcgttatc 82980

tatcctatca aagacaggtt tgtaattagt atcgataatg attgtcgcat ggtttctatt 83040

attaatttta ttggagaaac acggtcttcc aatgacacaa ggttctgtca actttttaat 83100

attgtcagat ctaattttct ttgatccact acaggcaaaa tcaggtagtt cgctacagaa 83160

tacagatctt ttcaaatgca tgttagcgat aaatggatta ggtcctttat ccaatacatc 83220

tgttaaaatt gtttgacccg tctcaacaaa caggtcaccg atagcagact ttaacaaacg 83280

tttggttgtc gactttccag ttgcagtttc tccaaaaaag aatgttaaac atcctttggt 83340

agcaccgcat aaacaactag ataaagtttt ttcgtacaac tctctatttt tcttattttc 83400

atccgttaat ggttggatat cgttaatgat attcattaac tcttccattt ctggactgtc 83460

ttcgacgaac tttgtatcgt caaatttaaa tccggttgat acagtacacg tatatttttt 83520

agcatcatct ccagagtaaa acattccgtc taccaggtcc aatacaccat ttttaaacgg 83580

aagtttatcc ggataggtat cggtctccac tgaatctatt aacatgtctc gtatgttagc 83640

ttctacagtc tttcgtttcc tcggacagag taattcgctt gaatattcct taggtagttg 83700

atgtcttatt gacagaatta gttttgttat caagggttct tcgctgttaa atttccatga 83760

attattaatc caaactatat ggtctcctcg ttcggttaat aaaacagagt tagtgtctaa 83820

aattctttgt gcaatattaa acagtttatt accatccaac ggaacaattt taactttaca 83880

actatgtgga ttaccagttt tgtaaattct aatagcacca ttttccaacg atagttgatg 83940

cggatgttta tgcgatcgtt ttttacataa tgcacaaggt gttacgtaat ctatgaccag 84000

tggtaccgtt gtaaaattat tttcatcgag atcattaaag tttattatag aattaatgaa 84060

tatttttgaa actcttttta tagcgtcttc gaatgaaata aaccctggtt cccataactt 84120

atcaggaact aaatcctcca atcgtcgttg tagagaaaag taataactat tgttgttcat 84180

atccacgtaa gtgaataggt aatcttctat attatcatgc ggtggttgca ttacatgaat 84240

agtgtcgcaa tttggatttt tcctagtacc tacaacccga agagttgttt ttctcctata 84300

tacggcagtg tctatcgatc tggttagtgg attttcagat gatctactta attctaatag 84360

tgttcgtttc atagctatca atgtatccat agtggtatac gtgtctaaaa agataatatg 84420

aaaacttgtt ttatctctat ttgtagactt agtcaatgaa aaattagatc tcatggattt 84480

tattacattt tcatgaatgg caccgcattc tgtaaacgcg aatctagcta cacagtttga 84540

cacctcgata ataaaatctt gaatagccgt taaataatct atttcgtcta gacacgcgtc 84600

taaatctaca tccatgaaaa ttctgactat agagtatgct tcctcatccc taagactttc 84660

gaatagtgta cattctggat tattctcaat atatctttct aactcgtcgc atttaaatgc 84720

ttctacaaat cttggatctt cattttgtct gcacgctgac gggacaccta tagtcttaag 84780

aacaaagata acatcattac ctctaatagt tgcatccatt tagaacacaa gttaaaattt 84840

cactaaagca ttaataaata aacccttgag cccaatttat aggtgccttg ttgtctaatt 84900

ccagtaaaac gttgataatt tcaaatgatc tatctttctc gaattggcgg tctctagccg 84960

ctggatgata tccgactatg gtagttaccg gggattctaa ctttgcccgt atattcgaga 85020

aatctgtttt acccaaacaa taaagaacac taacgtgttt agttatatgc tgcagcagta 85080

acttggaaat cttatcccag tagatcgcgt gactttttgt ttctcctaat ttacaactta 85140

agtaataatt ccagggtata accccgtcta ttatattaag gttataacct ttataatcaa 85200

ttactccggt taatctagat atagatgaag ctatctcctt aattgatttt tttgtaaaat 85260

ttggtgattc gaacggtaca ccagttccat ctttcggata cggatctata ccacacacac 85320

atactcgttt atttctaagc ggttgtttca actgtataaa gaacttatca ggaataggcg 85380

acgtctcgtc tcgtagcagc caactggcta cttcgttata aaactctacc aattgactca 85440

ttactggttc ccaatcatcg tgataagtaa tagtatatgg cgcgtgtgat acagtcactg 85500

aattcattat atcaaattag ataccttttt atacgtatct agtacctttt cattttccga 85560

aacgccgtta aaccaagcga atacaaacgc cttaaactta tctctattat aacatattct 85620

cgttagattt aattcttcgg tatccttgta tctaactatt gttatatgtt tagaaactct 85680

atagtggctt ctaatcagat gttctaataa atattttaaa aatgaatctt gattaaaaat 85740

catatcattg accatttgtc tggctactga attacgataa tacggagaca ataacggatt 85800

agtcaaatag ctatcatctc ctaatgatgg aggcacctgt acaaatacca atcgtttatt 85860

cttgaacgaa tcaaacgtat aaatatcata aatgacaaat ttatcagtgt gcatatcttc 85920

agatatatga ttggatgtag aacaccatct aacttgtctt ccagcatcta taaattcctc 85980

cacctctata tatctaccac cgtgctgaaa actagaccca tttaattcga cgcataaaga 86040

tccatatgta tctggagtca caatatattc agaaaaaaat acatgctcct ttaattttgt 86100

taattttgat ctgataaagt cattgtgatt tcctaaaagt ataaatactt cgttatcgtc 86160

gttatcgact ttgggatact tattatcctt aactataaaa atgtccatca atatcgatat 86220

aaaaaaaata actgatttac tcaacagtag tattttattc ccagatgatg tgcaagaact 86280

tcttcgagag aaatatatag tattagaaag aaaatcaaat ggtacaccta cagtagctca 86340

catctataag acaatggcta gatttgataa taagagtata tatagaatcg ccaagttttt 86400

atttatgaac aggccagatg ttatcaaact tttattttta gaagacgtag aacctctgtt 86460

acccgacaaa agtattaata tatctattaa caatacagag tatccacagt tggaaggtcc 86520

tataggaaca aaaatcgctc tattggaatt atttaatgca tttagaacgg ggatatcaga 86580

acccatacca tattattatt taccgcttag aaaagacata aacaacatag taactaagta 86640

agtcttcgac atctaaacct tcacatttaa tggctcctct atttagttcg aaaaagtttt 86700

ttgtagacgg tctatcttcc attgtagatg cgccattaat aaacttttta cttcgttcta 86760

taattgtagc gaaatcaacg ttatctacaa gaacaaatcc gtattcgtta aacactctga 86820

ctatatcgtt ctttttgata atgtattcag tcattggagt agacattgtt gatggattat 86880

ataccactat tctatcatca gctatttttt ctacagacat atagttttcg ctactaggta 86940

aattcttatg aattataaaa gtctttttat ctgttaattt tgataatttg tctccgtcca 87000

tggtagtgat taataccttg cctccagaag cagttagttc ggataagtta ttcatgacgg 87060

tagcataatg tctcggatga aaagaataat ggatagcaaa ctgccagtcg atgatattaa 87120

actttccaaa atagaatact tctctgacac tagagacaaa tgtatcggat cgaatagttt 87180

cctgaatgta gtcaaatttg tagtacttgg ttttaattcc agagtttaat ttgttgtatc 87240

tttcatttcc tctagctata gcatcagcat ccggatccgt cgctaccaat aacgcaatct 87300

ctccataaaa gtatttttcc aggtccgcac cgtttccaaa atcaatcgcc aatacctttc 87360

gtttgttgga atcgtctaaa aatgttttgg aacaatacat agaaataaga agagtcttga 87420

cgtagtttga taaaattccc aacggtcctc tagttcgttt attcgtaaaa taactaactt 87480

ctggatttaa tctaaattta tcattattgg cgtattgatg tcccacatcc gatagtttat 87540

cctcgttaaa gatatctcct attttgatgc tttgatctct taaatgttcg actatgatat 87600

tatgttgatt tccataataa tcttctgagt taatatattt catggtttta tcaattctag 87660

gtttaagtat ttctccatta actaagaatt ctgctataaa cttaataggt acaaccacgg 87720

acttaatacc cacttcatta tgtgtattaa tatattccaa acaatagata ttatttagat 87780

aattaacgcc gttatataac acaatcttac cagaaccata ttctttagga aagcctttat 87840

cgttgctaaa tttcttatac tctacaaaga tagacgattc tccaaagata attggttcac 87900

tggacatgta cctaaatact acatttgcag tttggtctat agtattttcc tttttaattt 87960

taaaatcaat gttagattta ggtccctttg aatagaacag aataacacct tctggttgct 88020

ttggtaaata tgtagataac atatcgacga cttcactagt tgtagtaaac ggaccttcgt 88080

atttctttga cttgaatact atccgatcac aaatatccac tagtttagat tcaacatact 88140

tactttcttc tagtctatca ttgattgcat tcacaggctc tattagctta atgagatata 88200

cggtccagtt cttatcctta actgcctcac caaagactac tacttcggaa tctattattc 88260

tcttaacagg atatctaata atataaccaa gatgtgtaaa ataacaatac aacccttttg 88320

atgtaactct gatagttata ggaattccgt cagtcttagt tacggcatat agattttcca 88380

gatccaaacc tactatatct tgtttaggca acataaaggt ttttataggc gcgttaatag 88440

gcggagaaag aattacattc tctggagaag ccataaatat atgacgtgat agagtcgtca 88500

attcctttat tagttcatca tatggaactt tttcattgtc tctaggtgtg aattctattt 88560

ccagagatgt attaggcctt gactttggat gattaatagc gtgtaataaa gaacttttag 88620

actgggcacc ggatcctaga aaatatttta gcttaaagtc tatcgtaaag ttttttgttt 88680

ttgcttgaat aagattgact aattctagtc ggatagagga tccatatttc ttgtaatcca 88740

aaaatatatg acgttcctct gtcgatagtc tcaacaagca ttctttgtga agacgatttt 88800

ccgtcactaa tgatttcttt tcccaaacta tgttatctat agcatctact aactgtacat 88860

tttttacatc tagaccatgt accttagata atggaatctt agttctaatt ttaacacctt 88920

ccttattagt aacggtaaat cgaataaacg attcctgaat cgtagagata ttcactacat 88980

ttgtcaaagt aattaatggc ggcttaataa atactagttc caattcatta tttatttcgt 89040

atgcggtaga cctctgttct aattctgaag cattcttcgc taaagcgtct atatacgttg 89100

caatagtaga agatgatact atgttggcat ccatattgtg ttttattata aacgactagt 89160

tttttttcat ttacttatta acaagcgtct tttatatatt cgtaatctat gcctttagct 89220

agagctattt taagcttttc tgtatctcga ctgatacgag tatctgatct attgcggtat 89280

ttttttataa atcgtttaag acggggagta gttttgatat attcgctaat atcctcttta 89340

ataatatcca cacacgccaa ttgttcggct atactcgatg cgtgagtctt gcattcatct 89400

ccagatatcg gagtgagggt cagatccaaa tattgagaag ccttataatt atcgtattcg 89460

aaatcaatca taaactgacc atccttatcc actgaaaaaa tggtattgtt tggatggcgt 89520

ttaaatatag acattatcaa tgccataata tctaatgtgt ttagctctcc gaagaaagct 89580

gtcattgcga gagatttcat acgcttatcc atttccattt atcggtcttg taattatttg 89640

tgtaaagatc tatatcatcc atccgtagat gatttaacgt gatctacgac tatagatagg 89700

aattcatcga acgtagtttt agatactaca tctaaaaaat ttttatcctt taccatttct 89760

aaaatagtcg ttgccatata agctcttttt gaaattgatg gagtatgacc taccacttca 89820

gcagtttgtt tgatagttaa cgctattaac ttttttggtg acggaagagg agatatggac 89880

tttacatttg tccaaaaatt atataaaaac gtataattga ctccatacgt tcggagatcc 89940

ttgattctaa taccaaactg tttgatacat tcgtatacct ttcgttcact tagtttgttg 90000

aacagaaatt cttcgggact agaatcatcc gtcagtttca atagcggttt atatagtcta 90060

ttagacttat gaacaacaaa ttcatgtgaa actttgtcct ttcctacaaa cttgataact 90120

atttcatcgg gacttatttc tatgtgttta ttttttagtg ttaataaccc tactgtttca 90180

ttctccttaa gatatttcat tttaccaaat ctaataaaaa acatagtttc cattaacata 90240

aaaaccgcca actgataatt ggaatctgtg gacgatttct ttatattttt gtttataaaa 90300

caattaattc gtttcataac gttatatact ctaacaaaaa tacgatctct tttagcgttg 90360

cgattctgta catgcatttt tccgtaaaag tattgtctac gtccttttga atcacttccc 90420

acaaaaatta atctagttaa cgcctcctcc cacgtttgtt catatactac tacatctgtt 90480

aaatgagtag gaattttaac atgttgcaaa acctcatacg ctggattatc gtctgataca 90540

ggatttaaaa aattattatc ggtaaagagt ttaccatctt tataaaaaag tgcacgcatt 90600

tacttcttac aagttttaac ttttttacga acaactttag attttccctc ggtgactaga 90660

tcagatagtg ttgtaatagc tttagtcata gaagtaaatt gtctagagat accagctgct 90720

tgaacatcct ctagaaccgt cgatactgca gagattctag taataatttt cttaagatct 90780

ttaacgatat tgtcggtagc cacctttagg tcagaaagat cgcttctagc actatgattt 90840

actttaccag cttcaacttg taccataggt tcatcatccc cgtcgctatc atcgagctct 90900

acagcagcca cgctttctac aatgtcgctg actccaggag atggagaatt tttttcagtt 90960

gtttgatgat attcctctat aactacttct tcttccactt cctccttttt ggttgatctt 91020

ttagtagccg ctggtttacg aggagtagtg gctcgtttgg ttttgggctt agtagatgga 91080

attattacat cttccgggaa aatatcctcg tttttatctt tattttcagc gctatttttt 91140

agatgagctc tgatttcagc catctttgtg aagctactag tatccgcttt atttgtaatt 91200

gaccacgcca ttacgataca aacttaacgg atatcgcgat aatgaaataa tttatgatta 91260

tttctcgctt tcaatttaac acaaccctca agaacctttg tatttatttt cattttttaa 91320

gtatagaata aagaatctat aaaaactaaa aaaattatac atcataaacc aatttcctag 91380

ttgtttgtaa ctttaaatgg actctaaaga gactattcta attgagatca ttccaaaaat 91440

aaaagcatat ctactagacg cgaatataag tccaaaatcc tacgatgact ttatctcacg 91500

aaataaaaat attttcgtta tcaaccttta taacgtatcg actatcacag aagaagatat 91560

acgattgtta tacactacga tagaacagaa tattgacgcg gatgatcaaa cactggttgc 91620

tattttttcg tatataggat ataaatttga acaggctgtt aaagaagaga ttagtacgag 91680

tttatccttc aatgacaaga ataccacaga tgaaatgacg tataacttgt atgatctttt 91740

ttttaacaca ttagacatgt atttacgaca aaagaagatc agtattctgg taaatgatga 91800

tgttagaggt gatgtaatcg ttagttataa aaatagtgac ttagtttcat catttaatgc 91860

ggaactagaa ccagagatta agaagatacc gttcaatatg aaaaatctat taccgtactt 91920

ggaaaagaat ttggaccaac taagattctc taaaaaatat ttagactttg catatttatg 91980

tagacacatc ggtattccca tttccaaaaa aaagtataat gtgcgatatg tatttcttta 92040

taaaatagac ggattatcca ttcctattat cattaaggat tttttagatg ttaagtacgt 92100

atatttggaa aatactggaa aaatttataa aaattctttt tccgaagacc ataacaacag 92160

tctatctgat tggggtaaag tcatcatacc tctcttaaag gatcgtcatc tatatagcta 92220

catctttcta tctagttatc atttacatag ttactataca gatctcatcg cgagagacga 92280

gcctgtgttt gtgaaacgca aaaaactaga tattatagag atcgatgaac ctgaggcatg 92340

gaaaagggat gttagagtgg aattcgcacc gtgtgagcat caaattagat tgaaggaagc 92400

tatgaaagtt gacgctaact atttcactaa aattaataat tttgctaacg aatttattta 92460

ttatgaagat ggtgtggcat attgtagagt gtgtggaata aatataccta tatttaattt 92520

agatgccgct gacgtgatta aaaatacagt tatcgtttcc acgtttaaca agactatatt 92580

cttgagcgaa ccatatagct atttcgttca tagtcagcgc tttatcttta atattatcat 92640

gtcttttgat aatattatga aatctcaaac ttgggtaatg aaatacaaca ttaaccgact 92700

aattcttaac tttcttattg atataaactc tagacgtcag gaatacgaaa aaaagttttc 92760

ttctgaaatt aagagaggtc tgttctttct tcgtttgtct gcaaacttat tcgaaagtca 92820

agtatcgtct acagagttat tttatgtttc caagatgctt aatttaaact atatagttgc 92880

gttagtaatc attcttaaca gtagtgcgga ctttatagtt tcttatatga aatccaagaa 92940

caaaacggta gaagaatcca ctcttaaata cgccatctcc gtggttatat acgatttttt 93000

ggttaagact agaatttgcg agaagggatc gttggatact atagttttat ttaccgatgt 93060

atacacatct ataatgccgg aggaattgga tttacatttt cagagaatca cattagaact 93120

tagaaaacta gtatccattc agagatcggc gttagaaccc aattacgatg tagaaagtcg 93180

cggcgaagag cttccattat ctgcattaaa gtttttcgat acaagcacca ttatagttaa 93240

gacaatggct ccagtacata catacatcga acaaaaaatt gttgcaccta ctccatcggt 93300

cgaaccaact gatgcatctc ttaaaaactt caaagaacta acgtgtgacg aagatattaa 93360

gatctcgatt agagttcatg atactaatgc tacaaaatta gtcatttttc catcacatct 93420

aaaaatagaa atcgagagaa aaaaactaat tataccgcta aagagtttat atattaccaa 93480

tactctcaaa tattattatt ctaactccta tttatacgtt ttcagattcg gagatcctat 93540

gccattcgaa gaagaactca tagatcacga acatgtgcaa tacaaaataa attgttacaa 93600

tattctaaga tatcatttat tgccagacag tgacgtgttt gtatatttta gtaattcatt 93660

aaacagagaa gcattggaat acgcatttta tatctttttg tcgaaatatg taaatgtgaa 93720

acaatggata gacgaaaata taactcgtat taaagagttg tatatgatta atttcaataa 93780

ctaaatggcg gcggtgaaaa ctcctgttat tgttgtgcca gttattgata gacctccatc 93840

agaaacattt cctaatgttc atgagcatat taatgatcag aagttcgatg atgtaaagga 93900

caatgaagtt atgccagaaa aaagaaatgt tgtggtagtc aaggatgatc cagatcatta 93960

caaggattat gcgtttatac agtggactgg aggaaacatt agaaatgatg acaagtatac 94020

tcacttcttt tcagggtttt gtaacactat gtgtacagag gaaacgaaaa gaaatatcgc 94080

tagacattta gccctatggg attctaattt ttttaccgag ttagaaaata aaaaggtaga 94140

atatgtagtt attgtagaaa acgataacgt tattgaggat attacgtttc ttcgtcccgt 94200

cttgaaggca atgcatgaca aaaaaataga tatcctacag atgagagaaa ttattacagg 94260

caataaagtt aaaaccgagc ttgtaatgga caaaaatcat gccatattca catatacagg 94320

agggtatgat gttagcttat cagcctatat tattagagtt actacggcgc tgaacatcgt 94380

agatgaaatt ataaagtctg gaggtctatc atcgggattt tattttgaaa tagccagaat 94440

cgaaaacgaa atgaagatca ataggcagat actggataac gccgccaaat atgtagaaca 94500

cgatcctcga cttgttgcag aataccgttt cgaaaacatg aaaccgaatt tttggtctag 94560

aataggaacg gcagctgcta aacgttatcc aggagttatg tacgcgttta ctactccact 94620

gatttcattt tttggattgt ttgatattaa tgttataggt ttgattgtaa ttttgtttat 94680

tatgtttatg ctcatcttta acgttaaatc taagctgtta tggttcctta caggaacatt 94740

cgttaccgca tttatctaac actattccat attactaaaa tcggaacacc aatgcggtga 94800

cataaaataa ccgctataac ctaattcatt taacatctca ttaccacaag taataacatt 94860

attagacttg tgttttatca aatactgaca aaattgttga gcagatggat cgacctttgc 94920

cgccttttta accatccacg cgtctccagt acctcgccta atagcttgcg gcagatatgt 94980

tttcttatcc aatcgcatag ctataaaata ggcgccgaaa tccacacatt tgaattcgaa 95040

tatatcatcc ttaccagcgg ctagaagtct acctctatca ctttctaatt ttgttttgct 95100

atccgttaat gatttccaat cgttaaccgt atttttaatt cgcatatatc tcgttaattc 95160

attaaagact ggattatcag acgtctgaaa ccagagtaat agcgcactaa ttgccaatat 95220

aataacaaag aatataagtg ttgatgtttt ggctgcttgt acgcctacta tagccttttc 95280

tctaacgtat tctaaattac acgcgtttac cgataaagta gttttatcca tttgtacgtt 95340

ataaatggat aagaaaagtt tgtataaata cttactacta cgttcaactg gagatatgca 95400

caaagccaaa tctcccacta taatgacaag agtaaccaat aatgtgtatt tgggaaatta 95460

taaaaatgct atggatgcac catcatctga agttaagttc aaatatgttt taaatttgac 95520

gatggataaa tatacattac ctaactctaa tattaatatt attcatatac cgttggtaga 95580

tgatacaact accgatatta gtaaatattt tgacgacgta accgcctttt tatctaaatg 95640

tgatcaacga aacgagcccg tgttggttca ttgtgctgcg ggagtaaata gaagcggggc 95700

tatgattttg gcatatctaa tgtctaaaaa taaggagtca ttgcctatgc tatatttttt 95760

atacgtgtat cattctatga gggacttgag aggcgcattt gtggaaaatc catcgtttaa 95820

aagacagatc atagaaaaat atgttattga taagaattaa aagtcttctg tttcttccat 95880

ttccttgatc ttttgagaag atatcttatc agatagtttc ctttccattc tcatgagaag 95940

acccaagtcg ataaagtatt tgtaatatcc agttcctata ttagggacct tgctaaaaaa 96000

gtggcagcta ctattatcgt taataggttc tgacttttta tgaagagccg cgtttaacaa 96060

tgctttattg tctccgaacg tagctctctt aagagtacta gctgcgccga acttgaattt 96120

attcactgat tctggttcgt aactagcaca tagtaaacta gcgagaagat cacaaggctg 96180

atacagataa tcgaaccctt ctccataggt gtttaacatg gcttcgcaca agtattcacg 96240

agcggcctcg ataccgaaga tatcgtatgt attccatact ccaggataca cgttgacgtt 96300

ttccaaatcg aaagaaccca attcttttag attcatgagt tctacagtca tcttattgag 96360

cttttttgtt tgattgaagt cgtcatatcc cgtataatca gagataggaa tcttgaattt 96420

actaatcttg cccttgttgg cggcacccgg aagaaccatc ataaacttac taagattcaa 96480

ttcttccggt tcaacgaaat ttaggtagac agtaaatcta atattatcct catcctcaat 96540

gaatgtttcc ataccccatt cctttacaat gacgctaaag gagatgaatc gttcaatcat 96600

atattcgacg actaattcgg taatttctgc tctcttgatg tataatctat tgactattat 96660

atctactaca tacctatctg tttcttttcg aagagtgatg tttggattta attctcccaa 96720

acatacaaat tcgaaattaa tcttaacaga ttgaagttta gagatatcat cggataccag 96780

agtgataatt tcggtcttat tcttactcaa attagtcaag ttattaaact cgttgaaacc 96840

aagtttttgt ttgacggcac cacttttttc agtagtgtga aaactggaca gggcttgttg 96900

tgtaaacttc tcagacaata cctgtgcaga aataattcca ataggagttc caccacctag 96960

agtataattg agtttttcat agaacttttc aaagatagtg atagccgttt cttttgtaat 97020

tctaattcta gaaggattaa gatgcgtcaa gaatatatac tccataaaat ctatattaga 97080

taccgtaaag aagtatttct ctctcacatc atcaatgacg ttatgaatca tatcgtacag 97140

atccttaacc ttaatagcat tatcctcagt ggtgggtttg acgaatacta ggaaattaaa 97200

cggcgccaat gtcttttttg caagtttctg tttctgagag taaacgaatc cctgttttat 97260

tttattccac agagcactaa tttccaaata ccaagtcatg gactcatctg gatagataag 97320

atctacaggt ttacatactg agcctagaat tttggtataa ttggcggcgt acttgatgag 97380

cgtattacct ataactactt gtccgtatcc gtcgaccacc atatcctcca tctttttaat 97440

gatttttcta gccagtgttc cggtacgtga tgtttcacag acgatatcag tagattgaga 97500

tcttgcaacc agcatcgaaa agtaatattg agaacccgtt aatccttttg ttaaagaatt 97560

aagaatgtaa cctcttcctt ctggatcctt agagtctgga agatagtaag gtaagactct 97620

acccaatact cgagtctctg ctggttcacc atcaatcctc tgttgtccat aagtacctag 97680

aatatacatt agttctgtgg gatttacttt ataacccgct ttggccattt tcaggaggtt 97740

attatctgga tcatctatca gcgtttgtct catatgttct tctatctctc ggatattaag 97800

atttgtcaag ttggataaca tggattccac atagtccgcc tctaaagctt tagataatgg 97860

aactattttc ccgtctctta catcgttgag atatttggcg tatgcttctt tgataagttc 97920

tattttttct acgttgatgg cctccaattt attagtgaac gtcgaatttg gtctcagatc 97980

tttgaatgtc accccaaaac cgtaaataga tagatatctc ttaaaaacat aagatgactt 98040

gataataaag ttgatacctt ccacgttcga cttatgatcg gataagagtc cagccaatga 98100

cagatgcctc atagccacaa caaaattact atctacgtcg tttgcaataa tttcaccatc 98160

ctttaagaga cccggataat taatcttttc acctatcaag taagtataga tatctttacc 98220

gctgaattta catttacctt taggatcgaa ctctcttcca tatttcccca agatgttcaa 98280

tacttcatct aaacaaagat cttgtatcct aaacaatgaa tacgctgcta cgatttcatc 98340

ttgaatagat ccataaacgg gggctccatg aatatcgtgt ttgagtaacg tcgtcggata 98400

cataagaata ctttgttcaa ttacggcttt aggattttgc tccaatatca tccattcttc 98460

atctccgtcg aaatcagcat tttgagagtt gacaattccg ggagatattt tgatagtatc 98520

tccttcggta gctctgatag atgaagcgat gacgttgtat ctatgtagag acggctgtct 98580

tccaaaaata atacttgtat attcttgaac agctacttct acccaatcac caggcaataa 98640

atgtatttta tttttgataa actttccttg gcgtattctt gttaattggt ttaatcgttt 98700

attaaagtaa aatttaactt ggtttgacgc taatagttgt ttaactttat ccactgtaaa 98760

ggcattaaca aatatctttt ctgtaagtgt atttctaata tatgcgggca ttcctacctc 98820

attaacggtg atagatgtac tgggaccaat tacagatcta gcggtctgat cttttcgccg 98880

ggcgacgata taacttctaa tcatattatt tttgccggat gtgatatatg ataaattgat 98940

actggaagtg ttattagaaa taattttaat atcatcgtat tctattaccg ccttctggat 99000

aacctgttca tcagcattca agttacaatt cttaacgatc atacctaata agtaagttaa 99060

ttcattggtt tctttgggta tactatctat ccaaaaacta atagccggtc taataatcaa 99120

cggagggatg ggaaagtagt ctgtataaaa taagttagct ggatattgat gaatttctaa 99180

taatggccaa aacttttcat gaatagaaat taacttttga tagatgagag aattaggaac 99240

gttaatatca tccaacttgt tgacgaaaca aacctttttc tttgaaaaag taattttttg 99300

atacggttgc atacattcac tgttccaaca tgactttttc ttggataata ttttatcctt 99360

taatctccta agagcgtgtc ccgataactc ttttaggtta atatcgtcgg aatacggttc 99420

tcgtgaacgc aataatccgc agtgaataca tatataattc agtaaacgaa taatttctga 99480

aataaattca ggcttaacta tatgagtttt ataaatactt actttacccc agtgaccgaa 99540

acattccaat tccgttttcc cacaagtttt acataatgcc ccatccatag cacctagtct 99600

accatcttta acggtaccga tatcgtcgtc atttttaaca tgactaatga taatatctgt 99660

agcattaatc tctttttgat catatagact atacgtaacc ttagagatta cagccatttt 99720

tatcaagtca gtttctttta aagaaccgaa agtatacaat caaatttccc tttttattac 99780

aactataaaa taatagttat atttacactt taaattttta tcatgacgga cgaacaaatt 99840

tatgcattct gtgatgctaa caaagacgat atacgatgta aatgtattta tcctgataaa 99900

agcatagtac ggataggaat agatacaaga ttaccctatt attgttggta cgagccatgt 99960

aaacgaagcg atgcgttgtt accagcctct ttaaaaaaaa atataacaaa atgcaatgta 100020

tcggattgta ccatttcatt gggaaacgtt tccattacag atagtaaatt agatgtaaat 100080

aatgtttgtg attccaaacg agtagctacc gagaatatag ctgtccgcta tctgaatcag 100140

gaaattagat accctattat agatatcaaa tggcttccga ttggattact agcgttagct 100200

attttaatat tagcattttt ctaaacaaga tataagatat aaaatatatt attgattatt 100260

ataatgttct tatctcatct ctactaattg attaatcagc gactgaaata acagatctat 100320

cggctatctc tactccagtt accatgttat tgcggaaaaa tctaacaatt tttaatggta 100380

tattaggacg gtagagaatc ttgacaacta tttccgtctc taacattttg ggaagacgaa 100440

gagtcttttt accatcgcct gtttgtagta cactattaac tatattagtt tctgtagtat 100500

ctagtctaac aaagctagga aataagtcag atattaccac atctatgtat agaaaattta 100560

caggaaactt gttatgacct ttttgaagat acgatgttat aatgattggt tctccataat 100620

catcatcaac cggagtataa tttatggatg attcgttaaa tacctgaaac acagttttca 100680

tagaatcaat tttatgtcta acggttatta aagtaactaa atcattatac tctatatcgg 100740

tagtatatct cagtagtacg tttctattta ttactgcgta cggatctctt gctatttctg 100800

tttttagaca tagaattttt gctagatatt ttacgttgta ttggttcatg actaactttt 100860

tcagttgatg ttgttggaat atttaagaaa cgaaatatag attgttgtag aaatagtacc 100920

tttgctttag tagtaggaaa tgttttattg cagtacacgg tcctcagcat aaagtacatg 100980

tgaaaatagt catattcctg attaggataa tcaaagttaa caactacttt gttacggacg 101040

atcttattaa ggtagtacat ctttttttca taatttacag cgtctgattt ggtaactcga 101100

gtcagtctca tgttctcacc ggtataaata cttaataatc tcatttcagc tgaatatgaa 101160

ggagcaaaag gttgtaacat tttattaccg tgtgggatat aaaagtcctt gatccattga 101220

tctggaaacg ggcatctcca tttaagacta gacgccacgg ggtttaaaat actaatcatg 101280

acattttgta gagcgtaatt acttagtaaa tccgccgtac taggttcatt tcctcctcgt 101340

ttggatctca catcagaaat taaaataatc ttagaaggat gcagttgttt tttgatggat 101400

cgtagatatt cctcatcaac gaaccgagtc actagagtca catcacgcaa tccatttaaa 101460

ataggatcat gatggcggcc gtcaattagc atccatttga tgatcactcc taaattatag 101520

aaatgatctc tcaaataacg tatatgtgta ccgggagcag atcctatata cactacggtg 101580

gcaccatcta atataccgtg tcgctgtaac ttactaagaa aaaataattc tcctagtaat 101640

agttttaact gtccttgata cggcagtttt tttgcgacct catttgcact ttctggttcg 101700

taatctaact cattatcaat ttcctcaaaa tacataaacg gtttatctaa cgacacaaca 101760

tccattttta agtattatat taaaatttaa tcaatgttta tttttagttt tttagataaa 101820

aaatataata ttatgagccg acgtaacact ttctacacac cgattgatac atatcattac 101880

ctcctattat ctctatctcg gtttcctcac ccaatcgttt agaaaaggaa gcctccttaa 101940

agcatttcat acacacagca gttagtttta ccaccatttc agataatgga ataagattca 102000

aaatattatt aaacggttta cgttgaaatg tcccatcgag tgcggctact ataactattt 102060

ttccttcgtt tgccatacgc tcacagaatt caacaatgtc tggaaagaac tgtccttcat 102120

cgatacctat cacggagaaa tctgtaattg attccaagac atcacatagt ttagttgctt 102180

ccaatgcttc aaaattattc ttatcatgcg tccatagtcc cgttccgtat ctattatcgt 102240

tagaatattt tatagtcacg catttatatt gagctatttg ataacgtcta actcgtctaa 102300

ttaattctgt acttttacct gaaaacatgg ggccgattat caactgaata tgtccgccgt 102360

tcatgatgac aataaagaat taattattgt tcactttatt cgactttaat atatccatca 102420

cgttagaaaa tgcgatatcg cgacgaggat ctatgtatct aataggatct attgcggtgg 102480

tagctagaga ggattctttt ttgaatcgca tcaaactaat cacaaagtcg aacaaatatc 102540

ctttattaag tttgaccctt ccatctgtaa caatagggac cttgttaaac agttttttaa 102600

aatcttgaaa gtctgtgaat tttgtcaatt gtctgtattc ctctgaaaga gattcataac 102660

aatgacccac ggcttctaat ttattttttg attggatcaa taataataac agaaagtcta 102720

gatattgagt gatttgcaat atatcagata atgaagattc atcatcttga ctagccaaat 102780

acttaaaaaa tgaatcatca tctgcgaaga acatcgttaa gagatactgg ttgtgatcca 102840

tttattgatc gcaaaagctt tgcacaatct ttatacacta tcggtttact atttattgat 102900

aacgcagatg tttgagttgt catccatggt aatccataga tcattaattt atcgtcttca 102960

cacgctagat tagcacgtcg taatctatca ataggatcgg gtatttttcg tttaggcatg 103020

aagaacatat ttaattcaga tctaaaaaat acatatatta gaatgaatac aatgaatact 103080

tcaaataaaa ctattaatct gtgtttataa acacttaata aagaatgttt aaacgtgggc 103140

tctataaaca caggattaaa gtatacatta ggaacattct ccatttatag taatcaatcc 103200

tttgtcggaa tatctgttag aggaatattc tttttaacac attccaatag tcccagaaaa 103260

tcatttaacc taatgggttc ttgaagaggg ctagaactat acgattctag ttccttatta 103320

gtactaagtt tctctagttt atttttattg gtgaatccgt aaatggcatt caatctcatg 103380

gatgtggaag gagaatacac attcaggaca ttaattcccg catatttaga tatcaaagcc 103440

ttgttgtctc cgataggtgt atactcagtc gacgcggatt ccatattttc tttataaata 103500

ttaatctttt tacgagtttc gaaaatacac aagataggtg atgatccttg acggaaaata 103560

accatgttct tattctttct cctaccactt tttttaggaa tgacggcagt catatccgta 103620

ttattaatga taagaggcat aacctcattg ttaggatcca agaaatagtt ttcaatggct 103680

tcaatagtta gtttatcatc tcctagaccc aaattgtttc ttatattata cattgtggta 103740

aacattgccc ctgtgatatt ctcgttatga atcaactcca actcatattt tgacatcgtt 103800

ctaggtacta tattaaaaat agaaagcata gatcttggaa atttggattt tgcgccggca 103860

ataaccattt gtaaatcatc atactcagat atacttcctg caaaaaatat ggtatcttct 103920

tcgatgaggt tttctagcag tagactcatt tagagaagtt ttttttgtga taaatgaata 103980

cccgtaccga tgttacaaac gataatatag acaaaaatcc aaccaaacga ggtgatagaa 104040

atataccagg aagaaatgaa agatttaatg accaaaatag attcaacaac gatataccaa 104100

agcctaaacc aagactacag cctaatcagc caccgaaaca agataataaa tgcagagaag 104160

agaatggaga ttttatcaat attagattgt gtgcctacga aaaggaatat tgcaatgacg 104220

gatatctatc tcctgcctat tatatgttaa aacaggtgga tgatgaagaa ataagttgct 104280

ggtcagaact atcgtcgttg gtgagatcca gaaaggcggt gggatttcct ctattaaagg 104340

cggctaaacg tatttctcat ggatcgatgc tatattttga acagtttaaa aacagtaaag 104400

ttgtgaaatt aaccccgcaa gttaaatgtt taaatgatac tgttattttt caaactgtag 104460

ttattttata ttccatgtat aaacgtggca tatattctaa cgaattttgt tttgatctgg 104520

tttctattcc cagaacgaac attgtttttt ctgttaatca attaatgttt aacatttgta 104580

cagacatatt ggtagttcta tctatttgcg gcaaccggct ctatagaaca aatctaccac 104640

agtcgtgtta cttaaatttc atacacggcc atgagacaat agcccgtaga ggatatgaac 104700

actccaatta cttttttgag tggttgataa aaaatcacat atcgctattg accaagcaaa 104760

cgatggatat tctcaaggta aagaaaaagt atgctacagg agcaccagta aataggttgt 104820

tagaacctgg tacactggta tatgtgccca aagaagatta ttactttata ggcatatcac 104880

tcaccgatgt gtcaattagc gataatgtca gagtattatt ttccacagat ggaatagtgt 104940

tagaaataga agactttaat atcaagcatt tatttatggc aggtgagatg tttgttagaa 105000

gtcagtctag tactattata gtataaagta ataaaaaata gttaatgtga tgactagcgc 105060

caccaacgcc aacaacattt gataatttct acttactaga cgtaccgtaa aaatataaat 105120

tactataaca aataatagta tatcaataaa caacctaatt aatggtcgaa gtatagcagg 105180

acattgatgc tctagaccgt gtataacaaa atctacaaat ttttcatccg ctatattttg 105240

tttcactata tcgtctagac gatcagcgat aacttccatg ttaatctatt aaaatattat 105300

caatatattt tcagttttgc atatccgtgg tagcaataac catcggagaa gttctaaaga 105360

atgtgtccat gtaagtagtc caatggacgt tttccttatt ggctaaaata agtttgattt 105420

tatcattggt ggatgtgaac aacatacgct tggcatagta cataaacaac gctgccaata 105480

ttataacacc gataacaatc atataaaact gaactcctgt accagcaact tgtctaggtg 105540

ctatttgagt agtggcctta gtcgtcaatt gcatcaacgc tttaatggca caatttcctt 105600

tgctagatcc tgtattaata aattccaaat ttgttggaga tcctggggct ccgtaacatt 105660

catctatgat tacgttttgt atctttaatt tgttatcgac gaccgcgcta gaattacaag 105720

tctgtttcac ataattttca aaatctctaa caacagtgtt tacactcgtc tgaatgttta 105780

acgcagcagt aaacatagct ggtacgtatg ctttttgttc cggtgttaat ccactatatg 105840

tttctgtagc ggctgataac acagcatcca actgagcatc cgcgtccgca gagcacatat 105900

ttttaacagt gaggttacat ccatggtttt gtcggatata aaaatttccg atttctatat 105960

cacattttgt ttgagcacta gcgttcgctt cttgttctaa tttagacgag atacgttcgc 106020

tgagtgtatt caccgtcgtc tgtatgcttg ccgcggcacc catttaaata gctacaatta 106080

gtatccatat taccaagaga gataataaac tgatcaaatg caattttagg tcgaacgaat 106140

gtttaatatt atgttgaact acttttgctc tattgacggg aacagtagaa aatctatcac 106200

tattgcttaa tccacatgac aatcttaatg aggaagtttt atccatctgt aagttgttca 106260

cgctagtatt acatcgtaca atattgcaaa gtcctaaatt attataatta cgtgttagta 106320

agaaattaac attggcattc gaacactctg gatcccaaca ttctcgaggt tccgcatatt 106380

ttaatgactc ttctaactta tctctagtgg gataactaca tctcatatat ttctgtttaa 106440

agtccgcaga ctgttgtctt agaatataat cgatcatctc tttgctatct tctgtattgt 106500

gtgcgcgtaa atgatgcaaa aatgattcac atattggtac actagcatct ttactacata 106560

atgtttgcat cttattatac agatttatta acgattgttg accctctaca gttctatcac 106620

tcctattaaa ggctgaaccg atccactgat ggcatatgtt tctatcgaac gtatccccct 106680

gacaccagtc gaataaatca acatcgcatt ttccagtgtc gtgaacgtct ggccaacacg 106740

attctaatac tgcaccctct tcatacttat ctgatatctt tccatccttt ttccaataat 106800

gagtacgatt aaaagtgcga cagcaattgg gtgcggttcc attatacgat tgtcttaaca 106860

atgccgaaag accaccgggt cctgtgttga ctaatctaaa ctctggatat cttttcttta 106920

ctttatcctc tttatctttt gctagtggtc ctatatgcac atattctaaa agcttagcgg 106980

gagctatcac gtcatgcatt ttatccacgt ttaataacat ctcatcagtg ggtactcccg 107040

gaggcggatc ccgtttaggg agctcaacac ttactctgcc acccatattt atctcattga 107100

aagtattaat ctaaaaacgt cataaagatg ttgatcttaa aggattgaac tctatccgaa 107160

aacaacattc ctagaatgtt atcgtcatta tccattacga ttctagtttc aaaaacattg 107220

actctctttt tgaatcctcg tagtttgttg agagacgaga tagctatttt gaaagaaaac 107280

ttttgtagtt cttgagaaca ttcagtcata gaatattccc tggaaaacgc atcagtatta 107340

ccaggagtct tcataataat attgtcatct ttaaacataa tagccaaatg ctgatgctga 107400

ctaatacact tgataaagcc caacaaccat tctaaatgaa tgacggttct accacaacat 107460

ttttcttcat acttgtgaaa attaaacacg taagatttct tttgatctat acttagacaa 107520

atagtagtat ctgtcctaat aggcatcagt tccttgttac aatcgacact tactacatga 107580

taactagaaa gttttactag attattttcc agatcaggtt ctatatctat gatggcatca 107640

ttgtgaaaac tacacaaaca cgattttacc ttggacacag gaagattaaa cacaatgttt 107700

tcggctccgc ggtagaacac tgatgcactg agaggtataa tggcccaaat gtttacagat 107760

ccgcccaagg cggcaaaaat atacattaac tcatccgtcg agtctacatt tatagatact 107820

tcttcactga actctgaaaa atatgccaca atttggcgca gtttatcgat ttttatacgg 107880

atgctcattt taaatttttg taaattattt aaagttaaat ggctgcagaa cagcgtcgtt 107940

ctacaatttt tgacatagtt tcaaaatgta tagtgcaatc tgtattgaga gatatatcta 108000

ttaattctga atacatagag tccaaagcta aacaattgtg ctattgtccg gcatcgaaaa 108060

aggaatcagt gattaatggt atctacaatt gttgcgagtc aaatatagaa ataatggaca 108120

aagagcagct attaaaaata ttggacaatc ttcgatgtca ttcggctcat gtatgtaacg 108180

ccacagattt ctggagacta tataattcgt taaaacggtt tactcatact accgcattct 108240

ttaatacatg caagcccact attctagcca cgctaaacac tttgataacc ctgattttat 108300

ctaacaagtt attgtatgcg gcagaaatgg tagagtatct agagaaccaa ctagattcat 108360

caaataaatc aatgtctcaa gaactagcag aattattgga aatgaaatat gctctcatta 108420

atctggtaca atataggatt ttgccaatga tcatcggtga gcctattata gtagctggat 108480

tttctggtaa agaaccaatt tctgattatt ctgcagaagt ggaaaggcta atggaactac 108540

cagttaaaac tgatatagtg aataccacat atgacttctt agccagaaaa ggtattgata 108600

ctagcaacaa tatagcagaa tatatagccg gcttgaaaat agaagagatt gaaaaggtag 108660

aaaaatattt accagaagtt atatctacaa ttgccaatag taatataata aaaaataaaa 108720

aatctatctt tccggccaat atcaacgata aacagatcat ggaatgctct agaatgttag 108780

acacgagtga gaaatactct aaaggatata aaactgatgg agctgtgact agtccattga 108840

cgggaaataa tacaattaca acatttatac caatttctgc gtccgatatg caaaagttta 108900

ccattttaga atatctttac attatgagag tgatggcaaa caacgttaag aaaaagaacg 108960

agggaaaaaa caacggagga gtagttatgc atattaactc accctttaag gtaatcaatt 109020

tgccaaaatg ttaaattatt agaatcatat atctttatcc atctattatc atctgtgaag 109080

ttttggctca gaaaaatatc tttacctaat attcgtttag acgatgtatc aataccggca 109140

tttttataac attcagctac caatttaaaa caatacattc tattatgacc aaatccatac 109200

ggaataccca ataaagttaa tgacgtatca gctgctattt tcataacttg aatattctct 109260

aacatgtata cttttgcact aagatatcct tcaaaaaaac tatccaatgg gactattcga 109320

actccttttc tataggtaga ttcaactacc aagtgttccg ataataacgt accataataa 109380

atacccacat gtttttctat cgatggattc aaatgacttg ttaatgacat ggtataatta 109440

ataaaaataa tagaacctct aggcgcatat gtcttgataa aattaaccgg atccatatta 109500

gtttatatcc aatagaggtt gcactgttaa gttacgttga ggttctattt gtgtagataa 109560

ttttaaccta caacattcgt tctttacact gacgagtaca tcctttaatg atttttttcg 109620

tataatcaat ttatatcgtt cgtgagaaat atctttgccg cacgtagagc acacgagttg 109680

gaacaccatt tttaatctag taacctatat ttattatatt atcactttta gtattggtta 109740

ttaacagact atcgttaaag taaaatgaac tatttatact cagtagtaaa ttatccttat 109800

agatgccgaa cactatagtg ttacagtttt cgttagaata tccgaagatc gaagatatag 109860

attttataac atcgatatct gatttagtat tttcgaaatc tatataagat gataaagttt 109920

taacttctga aacatcaatc tccttgcgtg gttctaatgc atacattaat tggcagggta 109980

acggaacggt ctctaaataa gatgatttaa aattgttcca tttttcatct aatgcggaaa 110040

atataaattc ttgaacagtg cattgacaag gaggaacata cgagtagaca tcgtctaaat 110100

tagcgtaatc gtttataaac gtaacaatat tacgcacgtc tacggtagag ttagtctttc 110160

tataataatt tttaattgcc aaactagtga ctatattatc gattgtaaaa tcactaaaca 110220

attgtatttt gtttaagttg gtgggtgtta tagatgcccc atagagtcca ggaaagaaat 110280

cacatccatt cactaacgcc gttaattttg ctaaatagtt atcctctgcc gagggaacaa 110340

atttaaacag ttgagttaag ttttttatca tcttaggatg attatcagta gatgcaaata 110400

gcatagtatc ctgatcggta cttattatca acggccattc tcctgtggta gagaaatgtg 110460

ttttagcctc gagacacata acgaattctg cgtctcgttc atcacaataa aataatgtaa 110520

cattctcatt attgttgaaa tgtgttagta tctcattcaa tgatattttt atgttatcag 110580

aatcagataa atatatttga aatgtgagtt tatcgatttt taattgcatt tctgccttta 110640

tttcttcttc cataaatccg gtaacatttt gtatttcgga tgtacacttt tctaattcta 110700

gcatttttct gtccttggtt aatttagaat atttacgtct cttgtctcta acgtcttgtt 110760

taatttttat acttcctcta tcgataaaaa gggttacatg ccctcccttt tttacccatc 110820

cgtttacgta ttttatgaat accgtagtta actcttctaa gtttctgaca caattggcga 110880

cggctatata aatactcatt gtatccacaa atattccatt gtatactttg tataaattat 110940

catctaatat cgtcagtgat ttattttcca gcagtaacga ttttaagttt ttgataccca 111000

taaatgaaga acgtactgat tattttcggt aaaccgtatt gtagtatttg tgaaaatgtc 111060

agtgatgcag tagaagaatt aaaatccgag tatgatatac tccatgttga tatcttatca 111120

ttttttttaa aggatggtga ttcaagtatg ctgggtgacg taaagcgcgg aaccctaata 111180

ggaaactttg cagcgcatct atctaactac atcgtttcca ttttcaaata caatccacag 111240

acaaaacaaa tggcatttgt ggacattaat aaatccttgg atttcaccaa aaccgataaa 111300

tcgctggtga atttggaaat tctaaaatcc gaaatagaaa aagcaaatta tggagtttgg 111360

ccaccggtta ccgaataaaa tatcttcctg agtattttgt ttattgttcc gcttccttta 111420

tcaaataaat tttgaagtat ttgataattt attggatcga ctagttcatc tatacatttt 111480

ttggaaacga gaacatctac ctttttattt gttgttatat gcgacgttgt atacgccata 111540

aatgtaactt gagaaaatcc acaaagaatt aattttgaac cagttatggg gacgcgctca 111600

ttagaatatg tataaatctg aggagtgtat tccttgagga gatattctat caagtcatca 111660

tttgcttctc taatataaat attcctgtcc tttataacaa ctatgccttt tagattatct 111720

cctaatttat aacacggccg tatacacttg gaatacttgt acaatctaag atagactaaa 111780

tctctcagag ttgtatcatg gaattccata cttaattcct ccgataaatc tacatgttca 111840

aatactttag aaatatattt atggtatttc ctatctggaa agtaagttat caagtcatca 111900

taagatattt caaatcctct acataaggaa gacactatct ccaacgattc ctcatcttct 111960

gtaagtagga atttggacaa gttaaacaaa attagatctc taaatggcat ctttattata 112020

tcttatttta tttttgttat tcgtatgtat ttcttattat tttacatatt atccgaccaa 112080

taaacttcag gcagctgtaa tggaaacaga tagagaaaac gctattatta gacagcgaaa 112140

tgatgaaata ccgactagaa cattagatac agctatattt accgatgcat caaccgtcgc 112200

gagtgcgcaa atacacctat attataattc caatattggt aaaattataa tgtcacttaa 112260

tggtaaaaaa cacaccttta atttatacga tgataacgac atacgaacat tacttcctat 112320

tttactcctt agtaaatgat tgtcttaccg aataaagttc gtattttcat caacgatcgg 112380

atgaaaaagg atatctactt gggaatttct aatttcggat tcgagaatga tatagatgaa 112440

atcttgggaa ttgctcactt gttggaacat ctacttatat cctttgattc tactaatttt 112500

ttagcgaatg cttctacatc tagaagttat atgagttttt ggtgtaaatc cattaattca 112560

gcaacggaat cggacgcaat cagaacatta gtttcgtggt tcttttctaa cggaaaactc 112620

aaagataatt tttccctttc tagtatacga tttcacatta aagaattaga aaacgaatac 112680

tattttagaa atgaagtatt ccattgtatg gatatactaa cgtttcttag cggaggcgat 112740

ttatataacg gtgggagaat agacatgata gataatctta atatagttcg tgatatgctg 112800

gtaaatagaa tgcaaaggat atcgggatcg aatatcgtaa tttttgttaa gagattagga 112860

cctggaacat tggatttctt caaacagaca tttgggtctt taccagcatg tccggagatt 112920

attccttcgt ctattccagt aagtacaaac ggtaaaatag ttatgactcc gtctccattt 112980

tatacagtta tggtaaagat taatccaaca ttagataata ttttagggat tctgtatttg 113040

tacgaaactt accacttaat agactatgag actatcggca accagttata tttaacggta 113100

tcctttatcg atgaaactga atacgagagc tttcttcgtg gcgaggctat attacaaatt 113160

agtcaatgtc aacgtattaa tatgaattat agcgacgatt atatgatgaa catctattta 113220

aattttcctt ggctatcgca tgatttatat gattacatta cacgtattaa tgacgatagc 113280

aagtcgatac taatatcctt gacaaatgaa atatatgcat ctataattaa tagagatatc 113340

atagttattt acccaaactt tagtaaggcc atgtgtaaca ctagagatac ccaacaacat 113400

ccgatagtag ttcttgacgc aaccaatgat ggactgatta agaaacctta tagaagtata 113460

cccctaatga agcgtctaac atctaatgaa atatttatac gatacggaga cgcgtctctc 113520

atggacatga taactttatc attgtctaaa caagatatat cattaaaaag aaatgccgaa 113580

ggaatacgtg taaaacatag tttttcagct gatgatatac aggcaattat ggaatctgat 113640

tcgtttttaa agtatagtag atcaaaacca gctgcgatgt atcaatatat atttctatca 113700

ttttttgcta gtggtaattc catagatgac atattggcaa atagagattc taccttagaa 113760

ttttctaaaa gaactaaaag taaaattttg tttggtagga ataccagata cgacgtcact 113820

gcaaaatcta gttttgtatg tggtatagta cgaggtaaat cattggataa aacgtctctg 113880

gttgaaatga tgtgggatct caagaagaaa ggattaatat attctatgga atttaccaat 113940

ctattgagca agaatacctt ttatctgttc acatttacta tctacactga tgaagtatac 114000

gattatctaa acactaataa acttttttct gcaaaatgtt tagtcgtgtc tacaaaagga 114060

gatgtggaaa atttttcatc tctaaaaaaa gatgtggtca ttagagtttg atttttagtt 114120

attatctaca ggaacaaata tagtatctga aatcatattc atatatcccg ttagaggtct 114180

atgataatat atagtagcgt ttgttccgtt atagacaccg aataatattt tacaaaagtg 114240

tatatacgta tcatcatctt tatgtttaaa atttaaaatc ttaattcgta aatttagaga 114300

taaaatggct tcttgtacaa tactagttaa ttctcccgtc ctctcaaaat tatccaactc 114360

ctcagcgaga ataggactta gtacataaag tttagcatac tctatcatct tcatataata 114420

attggataat aatttattcc atgtttctgt actaatatcg aacgagtcta tatattcctt 114480

tgtacgccat agaatatcca aatttgtagg gattataaac aaatcttcgg ggagtgttag 114540

attaaactta ttagcgtaca atatataatt atgtagaaat tctgaatcta ttcgctgtat 114600

agacttcata taagacagat catagaaata tacgtatgtc ccaggattaa ctcttcctac 114660

tcttcctttt cgttgatctc tcatagattt agaaataaat tcttgcgatc ctccaaaagg 114720

agcggggaca aaaactctac ccatatcata aatgtgtgta acattgcgta tagtaacgct 114780

ggattccaaa taaggagtag aaataattat cgatacatta ggtgatgaat acactttttc 114840

taatatttcg tctatatcta agaccttacc atgaataata tacatatcat acggtaatct 114900

tttttctaaa tatgatttat attcgtgaca ctgtgcaacg gatgccacaa agactatacc 114960

ggatgatcca tcaggaggag tatacatctg tatagcagta actaaatttc tcttttcttc 115020

ttctatgtat gccattctgg aagatggatt tatcttatta tgaataaata cctcgctaat 115080

tttaaacagt gtatctccag gaatatgtat aaatgcggga ttaggtaaaa atacttttag 115140

ccgttccctg tcatcctcta acgtggcagt cattaaaaac atagaatcta ttttcgtatg 115200

atgctttctc gctactgcta taataatatc tcctatttga tcatgctcat gaacttcgtc 115260

tataataaga gtgccataac taaatagttt tgttagagat aacttatggg tagaaaatac 115320

aattccatat ttttttggtt gtttgtttat taattcttcc ggtatagatc cgtaccgtaa 115380

agaaatagga gatccatcta gtaccttaaa tcccaatgat tttaaaatgg tattgctatg 115440

caatctaact aaagctatcc taggaagaga tagaatgact ggtctttcgt gaaagtcagt 115500

gattttatct agagtagaga atccaccaaa taaataatta aaccaaagca ataacttggg 115560

tacctgtgac gtcttaccca ctccagttcc tccagttaaa actacaggtc tatgagaaat 115620

ccacgctgaa aatatctctc gttgtgcttt aggagttaat gaggccaatg gaattttgct 115680

aaatggatac ttattcccta gtacacctat agtatctgtt cgtttaccta cggaattgga 115740

aaatatctgt aaactattac cagcttctaa tagtcctctt aatatatact catcgaacga 115800

attgatttct gtatttgtta tacattttag aaaactataa cattcaaagc ttattgtgtg 115860

ttcactgtat atatattgct tcgcggttag gtttatgtgt gcgggttttc ctagtgatgg 115920

aggagatact ttttttccac gtgcttctgt actaactttg tatattcctt tatgttttac 115980

aacgtgtgcg ttatgccatc tatgttttat aactggaaac accgccaatg agaaactatc 116040

acgttccgtt ttactcatat tactgaattc atcttgtgaa tatttgtaag agaatacatt 116100

aacgcagttt ggaaaaaaga agatatctgg taaattcttt tccatgataa atggaaagat 116160

atacagattt agtaattagt aaaataccag aactaggatt taccaattta ttatgtcata 116220

tatattcact agctggatta tgtagcaata tagatgtatc taaattttta acaaattgta 116280

acggatatgt agtggagaaa tatgataaat ctacaaccgc cggcaaagtg tcttgtattc 116340

ctatcggtat gatgttggaa ctagtagagt cggggcacct gagcagaccc aatagtagcg 116400

acgaactcga tcaaaagaaa gagttaaccg acgagttaaa gacgcgttac cattctatat 116460

atgatgtctt tgagttacct actagtatac cgttagcgta tttctttaaa cctcgactac 116520

gggaaaaagt atctaaggcg atagacttct cacaaatgga tttgaaaatc gatgatttat 116580

cacgtaaagg aatacatact ggtgaaaatc caaaggtcgt caagatgaaa atagagcctg 116640

agagaggagc ctggatgagc aatcgaagta ttaagaactt agtctctcag tttgcttatg 116700

gatccgaagt ggattatata ggacaatttg acatgagatt cttaaactcc ttagcgattc 116760

atgaaaaatt tgacgcgttt atgaataaac atatcttatc gtatatactt aaagacaaaa 116820

ttaaaagttc tacctctaga tttgtaatgt ttggattttg ttatttgtct cattggaaat 116880

gtgtaattta tgataaaaaa caatgtttag tatcctttta tgactccgga ggcaatattc 116940

caactgaatt ccaccactat aataattttt atttttattc cttctccgat ggttttaaca 117000

cgaatcacaa acattctgta ttggataata caaattgtga catcgatgtt ttattcagat 117060

ttttcgaatg tacatttgga gcgaaaatag gctgtattaa tgtagaagtt aatcagctgt 117120

tggaatctga atgtggaatg tttattagtt tgtttatgat attgtgtact aggacaccac 117180

ctaagagttt caaatctctg aaaaaggttt atacattctt taaattttta gcggataaga 117240

aaatgacatt atttaagagc attctattta acttgcaaga tctatccctg gatataacgg 117300

aaacggataa cgcaggatta aaagaatata aacgtatgga aaaatggacc aaaaagtcaa 117360

ttaatgtgat atgtgataaa ttaactacaa aattaaatag aatagtaaac gacgatgaat 117420

aactttgtta aacaagtagc ttcaaagtct ctaaaaccta ccaaaaaatt gtctccgtca 117480

gatgaggtga tatctttaaa cgaatgcata atatccttta acttggataa cttttattat 117540

tgcaacgatg gactgtttac taagcccatt aatactccgg aggatgttct taaatcactc 117600

ttgatcatgg aatcattcgc ctacgagaag atgataatca aaggattgat aaaaatacta 117660

atatctagag catatattaa tgatatttat tttactccat tcggttggtt gacgggcgtc 117720

gacgatgatc ctgaaacaca cgtggtgata aaaataattt tcaattcatc actaatatct 117780

atcaagtctc aagttataga atatttaaaa ccatacaatg tcaataacct atcggtactt 117840

accacagaaa aagaattaag tattaatacg ttcaatgttc cagattctat acctatgtcg 117900

ataatttcgt ttttcccatt cgatacagat tttatactag ttattttgtt ttttggagta 117960

tataatgact cgtattgtgg aataagctat ataagtccga aagagagact accgtatatc 118020

atcgaaatat taaaaccgtt ggtgtcggaa attaacatgt tatcggatga aataggtaga 118080

acatcatcca ttagaatctt caattccact agcgtcaaaa aatttcctac taatacatta 118140

acatccattt gtgaaattgt ttattcgttt gatgaatcat cctttccgac gcctaagacg 118200

ttcactcctc taaacgcgag tccatacatt cctaaaaaga tagtttcact attggattta 118260

ccatctaatg tggaaataaa agcgatatct agaggcggtg tggatttcat cactcatatt 118320

aataataagc gtctaaacac aatcttggta atagcaaaag ataacttttt aaaaaattct 118380

acattttctg gaacttttat caaagagaat attatttgga agggtatcta tacttataga 118440

ataatcaagt ctagttttcc agttcctact attaagtcgg ttactaataa aaaaaaaata 118500

tgtaagaaac attgtttcgt caattctcag tatacaacta ggactttgtc acatattctt 118560

tgatctaatt tttagatata aatggtggat gctataaccg ttctaactgc gatcggcata 118620

actgtattaa tgcttttgat ggtaatttct ggcgccgcca tgatagtcaa ggagttaaat 118680

cctaatgata tattcactat gcaatcatta aagtttaatc gagccgtaac gattttcaaa 118740

tatataggac tctttatcta tataccagga acgataattt tgtacgctac atacgtcaaa 118800

tccctattaa tgaaaagtta aataattttt tttattacac caacaaaaat gtttgtcatt 118860

aaacgaaatg gatacaagga aaatgtcatg tttgataaaa tcacgtctcg tattagaaaa 118920

ttatgttatg gcttaaacac ggatcatata gatcctatta aaatagctat gaaggttatt 118980

caaggaatat ataatggagt aacaacggta gaattggaca ctctggcagc cgaaatagca 119040

gccacttgta ctacacaaca tccggattat gccattctag ccgccagaat agccgtatca 119100

aatctacaca aggaaacaaa aaaactattt agtgaagtga tggaggattt attcaactat 119160

gttaatccta aaaatgggaa acattctccg attatttcaa gtatcaccat ggatatagtt 119220

aacaaatata aggataaact caactcggtt attatttacg aacgagactt ttcatacaac 119280

tattttggtt ttaaaacttt ggaaaaatcc tacttgttga aaataaacaa caagatcgtt 119340

gaaagacctc agcacatgtt aatgcgtgtc gcagtaggaa ttcatcaatg ggatatagac 119400

tcagctattg agacgtacaa tctactttct gaaaaatggt ttacgcacgc ttctcctacc 119460

ttatttaatg cgggaactag tcgtcaccaa atgtctagct gttttctact taacatgatc 119520

gatgatagca tagagggtat ctatgacacg ttaaaacgat gcgcattaat ctctaaaatg 119580

gcagggggaa taggtctatc aattagtaat attcgtgcca gtggaagcta tatctccggt 119640

accaatggta tatcaaacgg tattattcca atgttgagag tttataataa caccgctaga 119700

tacatagatc agggaggaaa caaacggcct ggagttatgg ccatatactt ggaaccgtgg 119760

cattctgata ttatggcgtt cctcgatctt aaaaagaata caggaaacga ggaacataga 119820

accagagatc tatttatagc tctttggatt cctgatctct ttatgaaacg agtgaaggat 119880

gacggagagt ggtcgttgat gtgtccggat gaatgtcctg gattggacaa tgtttgggga 119940

gacgagttcg aacgattgta tacactatac gaaagagaaa ggagatacaa atctataata 120000

aaggctcgag tcgtctggaa agcgattata gaatctcaga ttgaaactgg tactccattc 120060

attctttata aggatgcgtg taacaaaaag agtaatcaac aaaatttagg aactatcaag 120120

tgtagtaatc tttgcactga gataatacaa tatgcggatg ctaatgaggt agccgtttgt 120180

aatctggcat ctgttgcctt gaacatgttt gtaatagatg ggcgatttga ttttctcaaa 120240

ctgaaggatg tggtcaaagt aattgtcaga aatctcaata aaattataga tattaattat 120300

tatcctattc cagaagctga aatctctaat aagagacata gacctatcgg tattggtgtt 120360

caaggattag cggacgcgtt tattctctta aattatccat ttgatagcct ggaagcacaa 120420

gatctaaata agaagatctt cgaaaccatt tattacggtg cattagaggc gagttgtgaa 120480

ctagctgaga aggaaggacc atacgataca tatgtaggat cgtacgccag taacggtatt 120540

ctacaatatg atctttggaa tgttgtaccg tcggatcttt ggaattggga acctctaaaa 120600

gataaaatca gaacatacgg tcttagaaat agtttattgg tggcacctat gccgactgca 120660

tcaactgctc aaattttggg aaataatgag tcggtggaac cgtataccag taatatttac 120720

actcggagag tattgtctgg agaatttcaa gtagttaatc cgcatctcct tagagtttta 120780

accgagagaa aattatggaa tgatgagatc aagaatagga ttatggcaga tggtggatcc 120840

attcagaata caaaccttcc agaagatatt aagcgagttt ataaaactat ttgggaaatt 120900

ccacaaaaga cgatcataaa aatggctgca gacaggggag ccttcatcga tcaaagtcaa 120960

tctatgaata tccatatagc agatccgagt tattccaaac taacgagtat gcatttttac 121020

ggatggagtc tcggtctaaa aacgggaatg tactatctac gtacgaaacc cgcatccgct 121080

cccattcaat tcacattgga caaggataaa ataaaaccac tggtggtttg cgattccgaa 121140

atctgtacat catgcagtgg ttaaacaaaa acatttttat tctcaaatga gataaagtga 121200

aaatatatat cattatatta caaagtacaa ttatttaggt ttaatcatga gtaaggtaat 121260

caagaagaga gttgaaactt caccaagacc tactgcatct agcgattctc tacagacttg 121320

tgcgggtgtt atagagtatg caaaatcgat tagtaaatct aatgcaaaat gtatcgaata 121380

cgttacacta aatgcttctc aatacgctaa ttgttcgtct atctctataa aacttactga 121440

tagtttatct agtcaaatga cttccacttt tattatgttg gaaggagaga ctaaacttta 121500

taaaaataaa tctaaacaag atagaagcga tggatacttt ctaaaaataa aagttaccgc 121560

ggctagtcct atgttgtatc aacttctaga agccgtctat ggaaacatta agcacaagga 121620

acgcattcca aattctttgc atagtctttc ggtggaaact attacagaga aaacatttaa 121680

ggatgaatcc atcttcatca acaaattaaa cggatccatg gtagaatatg tttcgactgg 121740

agaatcatcc attctcagat ctatagaagg tgaactagaa tcactcagta aaagagaaag 121800

acaattggcc aaggcaatta tcacacctat cgtcttctat agatccggaa cggaaacaaa 121860

aattacattc gcactcaaga aactaatcat tgatagagaa gtggtggcta acgttatcgg 121920

actctctgga gatagtgaac gtgtatcaat gactgaaaat gtagaagaag atctggctcg 121980

taatctggga cttgttgata ttgatgatga atatgatgaa gatagcgata aagaaaagcc 122040

aatattcaat gtataaatgg ataagttgta cgccgctata tttggtgtat ttatggggtc 122100

tccggaagat gatttgacag actttataga aattgttaaa tctgttctaa gtgatgagaa 122160

aacagtcaca tcaactaata ataccggttg ttggggttgg tattggttaa ttattatttt 122220

ttttatagtt cttattctac tactattgat atatttgtat ttaaaagttg tttggtgaac 122280

ttaaatggcg gaatttgaag atcaactcgt tttcaatagt atcagtgccc gtgcattgaa 122340

agcttatttc actgctaaaa tcaatgaaat ggtagatgag ttggtcacaa gaaaatgtcc 122400

acaaaagaaa aaatcacaag ctaagaaacc tgaattacgc attcctgtag atcttgtaaa 122460

gtctagtttt gttaaaaagt ttggattgtg caattatgga ggaatcctta tcagtcttat 122520

taatagtcta gtagaaaata atttctttac aaaggatgga aaactggatg atacaggcaa 122580

aaaggaattg gttttgacag atgtcgaaaa acgaattctt aataccatag ataaatcatc 122640

tcctttgtat atcgatatta gtgatgttaa agtattggct gctagactaa aaagaagcgc 122700

tacacaattt aactttaatg gacatacata tcatctggaa aatgataaaa tagaagatct 122760

cattaatcag ttggttaagg acgaatccat tcaactggat gaaaagagtt ctattaaaga 122820

tagtatgtat gtcattcccg atgaacttat cgatgttctc aaaactagat tgtttagatc 122880

tcctcaagtc aaggataata ttatttcgcg tactagattg tatgattatt ttactagagt 122940

tactaagaga gacgaatcgt caatctatgt gattctaaag gatcctagga tcgctagcat 123000

tttgtcacta gaaactgtta aaatgggcgc ctttatgtat acaaaacata gtatgttgac 123060

gaacgctatt tcatctagag tcgatagata ttctaaaaag tttcaagaat ctttttacga 123120

agatattgta gaatttgtta aagaaaatga gagagttaat gtatcgagag tggttgaatg 123180

tttgactgtg cctaatatta ctatatcaag taatgctgaa taaaaatatt tataaatatg 123240

ctcgtcgtaa ttatgttttt tatagcgttt gccttctgta gttggctatc atatagctat 123300

ctgcgtccat atatctcgac taaagagtta aataagtcga gatagtttta tatcacttaa 123360

atattaaaat ggccgaggaa tttgtacaac aaaggttggc caataacaaa gtgacaattt 123420

ttgtcaagta tacatgtcct ttttgtagaa atgcactgga tattctaaat aagtttagtt 123480

tcaaaagagg agcgtatgaa attgtcgata ttaaagaatt taaacccgaa aatgaattgc 123540

gtgactattt tgaacaaatt actggtggta gaactgttcc tagaatcttt tttggaaaaa 123600

cttctattgg tggatatagc gacctgttgg aaatagacaa catggacgca ttgggtgata 123660

ttctatcatc tattggggta ttgagaactt gttgagaaaa taatgaaaaa acaatactta 123720

atcatgtcgc cgacatgttc atgtatccgg aatttgcgag gaaggcttta tcaaagctta 123780

tttcaaaaaa attaaacatt gaaaaggtgt ctagcaagca ccagctcgtg ttactggatt 123840

atggattaca cggactattg ccaaaatcac tgtatctgga agctattaat tccgatattc 123900

tcaatgttag attctttcct cctgaaataa taaacgtcac tgatatcgtt aaggctctcc 123960

aaaattcttg tagagtagat gagtacctaa aatctgtttc cttatatcat aagaattctt 124020

taatggtatc gggaccaaat gtagtcaagc ttatgataga atataatctt cttacacaca 124080

gtgacttgga atggttaatt aatgagaatg tagtcaaggc tacatacctt ttaaaaatca 124140

atgcctatat gattaacttt aaaatagatc taacggttga tgaaatcatt gacttagtta 124200

aagatattcc tgtaggagct acgctacatc tatataatat attaaacaat atagatttgg 124260

acattgttct tcgtatatct gatgaatata atataccacc tgttcacgat attctgtcta 124320

aacttaccga tgaagagatg tgtataaaac tagttacaaa gtatcctatg gacaatgtta 124380

taaattttat taatcaagat gttagatata gtcccacctt catcaagaca attaaagatt 124440

ttgtcaacaa gcatcttcca accatgtacg atggattaaa tgattatcta cattctgtta 124500

ttatcgacga ggacttaata gaggaatata aaattaaatc cgttgccatg tttaatttgg 124560

aatacaaaac tgatgtagat actctaacat tggacgaaca gatatttgta gaggtaaaca 124620

tctcatatta tgattttaga tatagacaat ttgccgatga atttagagat tacattatga 124680

taaaagaaag aagacaaatc accatgcaat ctggtgatag aataagaagg tttagacgtc 124740

ccatgtcatt gagatccact atcatcaaaa aggatactga ttctctagag gatattctcg 124800

cacatataga taatgccaga aaaaatagca aggtatccat tgaagatgtt gagagaatca 124860

tttcatcttt ccgtcttaat ccttgtgttg tcagacgcac catgctgtct gatatagata 124920

tcaaaacaaa gattggaaat cttgtgctct gacactatca gccatcaaag gaattatggt 124980

aacagatacc atcaataccg tgttatccaa aattctgcat catcatagga atgtcttcaa 125040

gtatcttaca tctgtagaga ataaagaaat tgctgtctgt cgctcttcta tagagaatta 125100

aaaagtgtac gatgtgatct acgcacagat gatggattat tggataggct atacgatctg 125160

actagatacg ccttacacgg aaaaatcaat caaaacttaa tcggtcaacg atgttggggt 125220

ccgttgacag aaatgctgtt taacgagaat aaaaagaaaa aactaaataa tttaatggaa 125280

tacatcaaaa tatcagacat gttggtatac ggacactcta tcgagaagac gcttattcca 125340

attactgatt ctctttcatt caagctatct gttgatacca tgtctgtgtt aaatgatcaa 125400

tatgccaaga ttgtcatctt cttcaatacc atcatagaat atattatagc tactatctat 125460

tatagattga cagtcttgga caattatact aatgtcaaac attttgtatc caaagtgtta 125520

cacactgtca tggaagcatg tggcgtactg ttttcataca ttaaagttaa tgacaaaata 125580

gagcatgaat tggaggagat ggtggacaaa ggtaccgtac cttcttattt gtatcatctg 125640

tccatcaacg tcatttcaat aatattggat gatataaatg gaactcgtta atattttttt 125700

agaaacggat gctggaagag taaagtttgc cataaaaaat accgacgatg tatgtgcctc 125760

ggagttaata aataaatttg tggaactgtt aagtgaatac attcacattg accaatcaga 125820

attttatttg gtggtaaagg ataaggatat tttttatttt aagtgtgata gggggtctat 125880

ttcgattgta aacaatgagt tttatgtctt tgacgaaccc ttgctgtttg ttaaagattt 125940

cactaatgta acgggggttg aattcatagt tacagaaacc atgccgtgta gaattatacc 126000

aaaaaataat cacgcggtta tttcagtcgt gactaatcat aagttttata atgggttaag 126060

tttataaagg gttaaccttt gtcacatcga tcgcgtattt gggatcagat gccaaattgt 126120

taaataatct gatgaaaaaa taataaatat aattcagatc atcgctagac atgacattat 126180

tgtcctctat agcgatagtc gcgtgccgtc tacatgcagg acatggaaga gtgctgacta 126240

tagtatatag ttttcgttta cacgcttcta tgttgccgtc taaacccgct tgcgaaagta 126300

ctataaaaat aatggtccat acggctcttc cccaatgttt gggattcatt taaatgaaaa 126360

tatatttcta aattctataa atggatgttc ggtgcattaa ttggtttgaa agtcacggtg 126420

aaaacagatt tttatatctg aaatccagat gtcgaaatgg cgagaccgta tttatacgat 126480

ttcctcatta cttttattac gtagttacgg acgaaatata tcagtcattg tctcctcctc 126540

catttaatgc gaggccgttg ggaaagatga gaactataga cattgacgag acaataagtt 126600

ataatctaga tattaaagat agaaaatgct ccgtcgcaga tatgtggttg atagaagagc 126660

caaagaaacg cagcatacaa aatgccacca tggatgaatt tctcaatatt agttggtttt 126720

atatttctaa cgggatatct ccagacggat gttactcgtt ggacgagcaa tatttgacaa 126780

agattaacaa tggatgttat cattgtgacg atccacgtaa ctgtttcgct aaaaaaatac 126840

ctagattcga tatcccaaga tcgtacttat ttctagatat agagtgtcac ttcgataaga 126900

agtttccttc tgtatttatt aacccaatct cgcatacaag ttactgttat atcgatttaa 126960

gtggtaaacg attattgttt acgctcatta atgaagagat gttaacggaa caggaaatac 127020

aagaagccgt cgatagagga tgtttgagga tacagtcact aatggaaatg gattacgaac 127080

gagaactagt tctatgttct gaaatagttt tgttacgaat agctaaacaa ttgttggaac 127140

taacgttcga ctacgtcgtt acctttaacg gacataactt tgatctgaga tatattacta 127200

atcgtctaga gttattaaca ggagagaaga ttatctttag atctccggac aaaaaggaag 127260

ctgtacatct ctgtatttat gagagaaatc agtctagtca taagggagta ggcggcatgg 127320

ccaatactac gtttcacgtt aataacaata atggaactat atttttcgat ctatattcat 127380

tcattcaaaa atctgaaaaa ttggattcgt acaaattgga ttctatatcc aagaacgcgt 127440

tcagttgcat gggtaaagta ttaaatagag gagttagaga aatgacgttc atcggtgacg 127500

atactacgga cgcgaaaggc aaagccgctg catttgcaaa ggttttaacc acaggtaatt 127560

atgtgactgt tgatgaggat attatatgta aagtaattcg taaagatatt tgggaaaatg 127620

gatttaaagt cgtactatca tgtcctactt tacctaatga tacatataaa ttatctttcg 127680

gaaaggatga cgttgattta gctcagatgt ataaggatta taatctaaac atagctttag 127740

atatggctag atactgtatt catgatgctt gtttgtgtca gtatttgtgg gagtattatg 127800

gagtagaaac aaaaacagac gcgggtgcgt caacatatgt gcttcctcaa tccatggtat 127860

tcgaatatag agcgagtaca gtcatcaagg gtccactgtt aaagctattg ttggaaacta 127920

aaactatctt agttagatca gaaacaaaac aaaagtttcc ttatgaaggc ggtaaggtat 127980

ttgctccaaa acaaaaaatg tttagtaata atgtattaat ctttgattat aacagtctgt 128040

atcctaatgt gtgtatcttt ggaaatctat ctccggaaac attagtcggt gtcgttgtta 128100

gtaccaatag attggaagaa gaaataaata atcagctctt gcttcagaaa tatccacctc 128160

ctagatatat tacggttcat tgtgaaccta gactaccgaa cctcatctct gaaatagcaa 128220

ttttcgatag atcgatagaa ggaaccattc ctagactatt aagaacattt ttggcagaga 128280

gagccagata taaaaagatg ctaaaacagg ctaccagttc aactgaaaag gccatctatg 128340

attccatgca atatacgtac aagatagtag ccaactcagt atatggtctg atgggattta 128400

gaaatagtgc tctatactca tacgcttcgg ctaagagttg cacatccata ggacgtagaa 128460

tgatcttgta tctagaatcg gtactaaatg gagcagagtt atctaacggt atgttacggt 128520

ttgccaatcc attaagtaat ccattttata tggacgatag agatattaat ccgattgtga 128580

aaacatcgtt gcctatagat tacagatttc gttttcgtag cgtgtatgga gataccgact 128640

ccgtgtttac agagatagac agtcaagatg tcgataagtc tatagaaata gcgaaagagt 128700

tagaacgact gattaataat agagtattgt ttaataattt taaaatagag tttgaggcgg 128760

tatataagaa tctgattatg caatcgaaga agaaatatac aacgatgaaa tactcagcat 128820

cgtcgaattc aaaatctgta cctgagagaa ttaataaagg tactagtgaa actagaagag 128880

atgtttccaa gtttcataag aatatgatta agacatacaa gaccagactg tctgagatgt 128940

tgtctgaagg acggatgaat tctaatcagg tatgtataga tattctccgt tctttagaaa 129000

cagatttacg atccgaattt gatagtagat cgtctcctct agaattattt atgttgagtc 129060

gaatgcatca ctcaaattat aaatccgcag ataaccctaa tatgtatttg gttactgaat 129120

ataataaaaa taatccagaa actatagaac ttggagaacg atattatttt gcatatattt 129180

gtccggctaa tgtaccatgg accaaaaaac ttgtaaatat taaaacatat gaaacaatta 129240

tcgatagaag ttttaaactc ggcagtgatc aaagaatatt ttacgaagtt tactttaaac 129300

gattgacgtc cgaaatagtc aatctattgg ataataaagt tttatgcatc tcattctttg 129360

aaagaatgtt tggttcaaaa cctacatttt acgaagcata aaataattta caacagttgt 129420

acgtcgctct ttgttagatt cagtttatcc attagatatt ctacggctgg agtaattttt 129480

gtagtaattg aatacccagg tacgtaaaac aataaaaaca ttaataccgg atcatacttg 129540

gtaaagtaca atatagtact aatattagct aatgtctcat aatcggatgg acttcttgct 129600

ctactaagat tagggtgatc tatatctttg atacttattg gaacgggacc ggtaaatata 129660

taattctctt tgagacgact aaacaaaggc ttatagtctc ctgtttgatg catataccta 129720

gccaacgcca ggtgtttggc attatctgta tagacgtatc tgaataatgt gtgtttagta 129780

atatcaaact gagtattttt aaaccagaac aagatattat agattggaat ggaagtcagt 129840

aacaaaaaac taataaactc gtttgttgga agggcagaaa actttcgaag atatgatacg 129900

tatctaccag agaacgatcc attagcaatg aattcagtat ttactttttc ttcatatacg 129960

ggaataggac ctttatatag agacatgaga aactcgaact ttttaagtaa tcccaacgaa 130020

atgggataat agtcacttgc atctaagttt gcgtatctac tactccaaat tgcagtttct 130080

ggtatccatc catacgcgta attatcaaat agatatgtgt ttttcattaa cggtctactt 130140

agacctctac taaaaaatgt ttcttgatct agaattcttc tttgtgcatt tttgtacacg 130200

tcgtcaaaac gcggaacggt ggcagccata atatttatat cctaccgctt tttatcaact 130260

atgttatgtc ttttagttag gttaatattc taataagatg caggtaatac atcaggttaa 130320

agtattagaa tgggattata cttatatatt tagtttatct ttcatcagat aactaaaaaa 130380

tgtataaaac agacgcgtta cattgcctat gctacatgag ttccatgtgc ggagattgtt 130440

aaagtttagg gtagagagtt gttctagcat ccattcattg tctttacatc cgttatatgt 130500

aattatgtca cccatggaca ttataggttt taatagtact tcagatactg gaataagata 130560

ttgttcgtat atgtgtttga taatcatttg ttgcgtttct ggagaacttt tctctgcatg 130620

atttttacat agctttacaa actcgtgatc actttttata atgagagatc tatagtcttc 130680

gtatctgtta cgaaaatcaa tatattcagg attattttct gaatcacttg attcgtcact 130740

aatatacata actatatgat caacaatttt ttccaaaatc atttctgcat tttctttatt 130800

cattaatttg gtgggagtct gaattgttgc agcagttccc atttatcaaa aatgtgtgat 130860

aattatatct atgtatatac aaattattaa taatatattt ggtctgttct atgatctacc 130920

gtgtcttatc aattgaagta tatatttctt atccgtcttg gttagatgga tgcttttatc 130980

caagaattct tctacatgat atagattatc tctcaaaaac ctttgaaata agacgatgat 131040

ggaaatatta taactagcta aaatagtttt gattagcgat ggaagaatac tagggttttt 131100

atctgtagaa aatcgaacga atgcggatac atcctccaca gacttaagat ggtttatcat 131160

tttaactaac aagtctcctt tataatcatc taacacaaga cctagacgag ccaattgtcc 131220

gtctaaataa tattttaaaa tgtttttgct aaacaagttc agccgtctac tcttggattt 131280

tatagatgac agtttcttta gtattccgtt ctctatgacc atgggatccg actcgtgcaa 131340

aaacaggtat gtattgtatt ccttaacata atacgacgta tcctcgttaa agatttgaca 131400

cttcttcata tcatccattg tatctaatgt gatatcgttc acctcataaa ttatattaga 131460

tatataactg ttattaatat ctaactcatc aaatcgttta atgtccgcag cactcgtaga 131520

gaataaagac ataattcgtt cattatcgaa atattcggga tctatttgaa caacaactct 131580

gaataagtcc tcgttaaacg ttctatcact aataaatgta ttaccgttaa taaaattagc 131640

tttataaaaa ctaataaaat attttttcca atcgtcgttt tttgcatcaa taatagatag 131700

gatatctcca ggaatatcat atatgatttc tagcagaagt tctctgatat ctttttcagg 131760

ctcaaagata taaactaact taagcatgtt tttattaacg cgtccttgtt ttatctcaga 131820

gtatatataa ctaagaattt tatcatacat ggtaatggcg tgtctattaa gaacaatatc 131880

cgcatcatta gctatacttt cgtttaacca cacccaatag ctagtcttat ctccgggaaa 131940

gatataatct ctaaacgttt ctgttagatt gctatggact attctaccag tttgttgaaa 132000

tagagtgtat atatcttgtc tatcattatc cgcaaactct atgcctgtat ctttcttgct 132060

taggtaagcc caaataagta atcttataac acttaatttt ttaacaactt tgaagtctgt 132120

tttcttaaac agttttaaaa aaatgatttc catatctttg atgggttccc ctccgtgaaa 132180

taatggatta agaactaagt cataatttac gcctataagt tctcctaatt cgtccatgta 132240

ttcggcaacc tctaaaaatt tttcatcttg tgttagttta tgcacggttt ccttgtacga 132300

ttgtattgcg ttactaaacg ataaataatt cttagtcaat gctttaacgt acaacgtggg 132360

tgcatcaaat gcgtgtcgta aaaccgcttc gtacacttta caacatcgta ccatatgtat 132420

gaaaacaaaa aaattggtat tagctaatac atccttagta ataacgtgtt gaggaaaatt 132480

gctaactaga tacttgagag ctagtacatg attgagggta aaattgttta ctttacttaa 132540

tgtatcatcc tttaaaaaat ctatattatt ttctactgtg tatataagat actttctacg 132600

aataaaatcc atttttatag aagagagtta tctatgatac tacactctta ttctaataat 132660

tatttttact attttatcac taataactat ttatactatc cattattaat ttaacaattt 132720

gaattagtat tggagaaaga tgaaatgcct attcactaac aaagtcaatg cactcttgaa 132780

gatttttacc aataatatgt agcggattct caaagtattt atttaccatt tctttacaca 132840

gtatcttagc cactggccta cgaacaggca tacatggttc aactatatca tgaacaatgt 132900

ttaattttag tgtatccaat ccaataaatc cattttctac atcatcatcg tatctgtacg 132960

ctttgtattc tccactcttg aacaaatctc taaacagtat cttcaacact ttttccacca 133020

attcctggga tgtcacacat tgttctacca tctgtttgat aaagagggta tcttcttcag 133080

attttccctt gattctatac atgggtttta ggttatacct tgcatctatt gtatatttat 133140

cagattcagc tatcaacatt ttatcaactt gtgttccaat tattctccat ttattagatc 133200

tgcattcatt attatagaat ctttcttcca ctaatattct aataagatta agtttgaaag 133260

gagagaagat cttgtgtttg attttactca ttggatgcat tctataacga atgaatctat 133320

gatcatctcc aaacaaattg ggaaacatgt aaagcagcag agcatatacc atcttactcg 133380

gattctgtga tgctttgccg atggctgaaa tgtccgagaa tagtttataa gttttctgat 133440

tatttggaat agatggtgct atatcttcta gagtagtagt cctaatcatt ctcttaaatt 133500

ttatgtatcc tagtttcaat gtctcgtaat gagtttgtgc tgctcttatt gtctgattta 133560

tttcttttac cattttggct ctattctgaa actttatcct cttcttatcc atttttattg 133620

ttgactccgc actatcgatt tgatactttc ttttcagagt aaagctatcg tcattgatca 133680

tcggacactg acttccactc attatgaaat tgtagccgta taaccacaat acaattatta 133740

acatatatat tcacttttgt taatatcaac ataataatga aaaaatataa atgaacaaag 133800

ttaatacata agtgttataa atggaaaatg tatacattag tagttactca tccaatgaac 133860

aaacatcaat ggcggtagcc gctactgata tccgagaatt actatcacaa tatgtggatg 133920

atgccaactt ggaagactta atagaatggg ccatggaaaa atcatcaaag tactacatca 133980

agaatatagg taatacaaaa tctaatatcg aagaaactaa attcgaatca aagaataata 134040

ttggtataga atactcaaag gattccagaa acaaactatc gtatagaaat aaaccgtcta 134100

ttgccacaaa tttggaatat aaaacactat gtgatatgat taagggtact agcggcaccg 134160

aaaaagaatt ccttcgctat ctcttattcg gtataaaatg cattaagaaa ggagtagaat 134220

acaatataga taaaataaag gatgtgagtt acaacgatta ttttaacgtt ctcgacgaga 134280

aatacaatac accgtgtcct aactgtaaaa gtaggaatac tacgccgatg atgattcaaa 134340

ctagagccgc tgacgaacct ccactagtta gacatgcgtg tagagattgc aaacaacact 134400

ttaagcctcc caaatttaga gcatttcgca atcttaatgt tacaacgcaa tcgatacatg 134460

aaaacaagga aataacagag attcttccag ataataatcc atctcctcca gaatctccag 134520

aaccagcatc acctatagat gacgggttaa tcagatccac attcgataga aacgacgaac 134580

caccagagga tgatgaataa aaaaatgata aaataaatta gttttattgc tggttgtgtt 134640

agttctctct aaaaatgtct aagatctata ttgacgagcg ttctgacgca gagattgtgt 134700

gtgcggctat taaaaacatt ggaatcgaag gagctactgc tgcacaacta actagacaac 134760

ttaatatgga gaagcgagaa gttaataaag ctctgtacga tcttcaacgt agtgctatgg 134820

tgtacagctc cgacgatatt cctcctcgtt ggtttatgac aacggaggcg gataagccgg 134880

atgctgatgt tatggctgac gccataatag atgatgtatc ccgcgaaaaa tcaatgagag 134940

aggatcataa gtcttttgat gatgttattc cggctaaaaa aattattgat tggaaagatg 135000

ctaaccctgt caccattatt aatgagtact gccaaataac taagagagat tggtcttttc 135060

gtattgaatc agtggggcct agtaactctc ctacatttta tgcctgtgta gatatcgacg 135120

gaagagtatt cgataaggca gatggaaaat ctaaacgaga tgctaaaaat aatgcagcta 135180

aattggcagt agataaactt cttggttacg tcatcattag attctgattc tagttatcaa 135240

taacagttag tagtttagtt atacattgaa tcatacatat taattttttt attgagatag 135300

attaaaaaat acaaattgta gtactattaa cgcgactagt atattctcta aagatgatat 135360

ctgtcacaga tattcgtaga gcgtttctag acaatgaatg ccatactatc acaaaagcgt 135420

ttggatatct gcacgaggac aaggctatcg cattaattaa aataggattt catcccactt 135480

atctacccaa agtcctttat aataatgttg tagaattcgt tccagaaaaa ctatatctgt 135540

ttaagccaag aactgtagct ccattggatt tgatatctac tataacaaaa ttaaagaacg 135600

tggacaaatt tgcctcacac ataaattatc acaagaatag tatattgata acaggagaca 135660

agtctctaat tgttaaatgt atgccttaca tgattatttc agatgatgat atacgattca 135720

taagagaaca gtttgttggt acaaattcta ttgagtatat tctatccttc atcaacaagg 135780

aaagcatata tagaatgagt taccaatttt cagagaatga aatagtcact atcatcaata 135840

gagatcattt catgtatgaa ccaatatatg aacatcaggt cttagattct gactttctta 135900

aaactatgtt agatagatac ggaatagttc ccattaattc tggtataata gatgaattat 135960

gtccagaagc tataatagag atattaatgg cagtagttcg tcctagggac gctatccgtt 136020

tcttagatat agtgaataag aatcaattga cagaagatag tgtcaaaaac tatatcatta 136080

atgatatcag aagaggtaaa atagattatt atattccata cgttgaagat tttttagaag 136140

atagaactga agacttggga atatatgcga atatattttt tgaggatgct atagatatta 136200

cgaaactaga catcacaaag acagagttgg aacatatatc gaaatacatg aattattaca 136260

ctacttatat agatcacata gttaacatca tcttacaaaa taattatata gatatcttgg 136320

catctataat agattacgtg caagacgtat taacagaaga attatgtatt agaatagttt 136380

gcgaatcaac aaaccctgtt cccgttacat ctcttcctat acattctacg ttagtaatgg 136440

ttatgtgtat acaaatgaaa tatgtcgata tagttgaatt cttagacgag atcgacatcg 136500

atactttaat agaaaaagga gcagatccga taaccgaata cacatttaca acaagatggt 136560

acaataaaca caatgatttg atcactcttt acattaagaa atatggattc tgtccaatga 136620

tgatgaaacg gttaatgttc gaatatccat tgactaaaga agccagtgat catttactta 136680

aaaccatgga tgaaaacagg ggagctatta tgttttttcc gcgtactatt tgcacacttc 136740

cttatctatt atgttgtaat tataaactaa ttcaaaaacc tattccattc aaagaagaaa 136800

atcgtaacat cgtatataag aaaaccaaca gagtattatg ctttgactcg ttggagaact 136860

ctgcgtttaa aagcctcatt aaaatagatt ctattccagg attaaaaact tataacatga 136920

aagacattac atacgaaaag tctaataata taatttgtgt taggtttata cctcaagaat 136980

caattcataa tgaagagcga agaataaaat tacagttatt cgacattgct agattggcat 137040

cctatggact atattatatt ccctctagat atttatcatc gtggacacca gtagtgaaca 137100

tgatagaggg aagagagtac actaatccac aaaaaataga atgtctagtt attttggatt 137160

tattttcaga ggaattcata gaatatcaaa atctgggcaa tgcggtatct aataaatatg 137220

aactggaata tactatatct aattatcaag ctgccataaa ctgcctaatg agcacgttat 137280

taatatatct agttctagga tcaatcagat cgatatcaaa aactgaagat tttgtattat 137340

ctatattaaa tatcttctat aaaggactga aaattaatga attactttct gaaccagtat 137400

caggagtttg tatcgaatta aataaaataa aagatagagc gagctctgga gacagtagtt 137460

ttatatttct taagaaaaac gagttatcaa aaactctatc gctctgtgaa aaagtttgtg 137520

ttgagaccat attagacaat aatcagagtt ttaaatcctc aaaatgaata ggaatcctga 137580

tcagaatact tttcctaata ttacattaaa gattatagaa acctatttag gcagagtacc 137640

tagtgtgaac gaatatcata tgttaaaatt acaagctaga aatattcaga aaataactgt 137700

ttttaacaaa gacatatttg tatctttagt aaaaaagaat aaaaaaagat ttttttccga 137760

tgttgataca tctgcatcag aaataaaaga tcgtatactt agctactttt ctaaacagac 137820

tcaaacatat aatataggta aattatttac gattatagaa ctacaatctg tattagtgac 137880

cacatacacg gacatattag gagttcttac tattaaagct ccaaatgtaa tttcatctaa 137940

aatttcttat aatgtaacat caatggaaga attggcaaga gatatgctaa attctatgaa 138000

cgtcgcagta atagacaagg caaaagtaat gggacgtcat aatgtatctt ccctagtcaa 138060

aaatgttaat aagttgatgg aagaatatct tagacgccat aataaaagtt gtatatgtta 138120

cggatcatat tctctatatc taattaatcc aaatatacgg tacggcgata tagatattct 138180

tcaaactaat tcgaggactt ttcttataga tttggcattt ctaataaaat ttattacggg 138240

aaataatatt atattaagta aaatcccata tcttagaaac tatatggtga taaaagatga 138300

aaacgataat catatcattg atagttttaa tattcgccag gataccatga acgtagttcc 138360

taaaatcttt atagataata tctatatagt ggatccgacg tttcaactat tgaacatgat 138420

aaaaatgttt tctcaaatag atagattgga agatctatcc aaagatcctg aaaagtttaa 138480

tgcgcgtatg gcaaccatgc tagaatacgt tagatataca catggtatag tctttgatgg 138540

taagcgtaat aatatgccga tgaaatgtat catcgatgaa aataatcgca tagttactgt 138600

tactactaaa gactatttta gctttaaaaa atgtctagtg tatctagatg aaaatgtgtt 138660

atcgagtgat atattagatc ttaacgccga cacatcgtgt gatttcgaga gtgttacaaa 138720

ttctgtatat ctaattcatg ataatatcat gtatacatat ttctcaaata ctattctcct 138780

tagtgataag gggaaggtac atgaaataag tgccagaggt ttatgtgcac atatattgtt 138840

gtatcagatg ctgacatctg gagaatacaa acaatgttta tcggatctct taaattcgat 138900

gatgaataga gataaaatac ctatctattc acatactgaa agagataaaa aacctggacg 138960

acacggattt attaatatcg aaaaggatat aattgtattt taggacaaaa gtctagaagc 139020

tacattatcg cgattagccg cgaacatatt ttgtagcatg tccgtcctca taaacggaac 139080

ttgttttcct gggttattca ttctctcgca tctggaaggg gacgaaggtc ttctctcaca 139140

cgcgggtgat gagggtttat ctactttgag tacgcaggaa gctttacttc cttcgcattc 139200

tacagttcta acatcgcata ctttaacggc ttttagaacc agatatcttc cttctttggt 139260

attgatataa aacggagtat gagcagatgc aaaatgagaa ttcatttata gcatagaaaa 139320

aaaacaaaat gaaattctac tatattttta catatatata ttctaaatat gaaagtggtg 139380

attgtgacta gcgtagcatc gcttctagac gcatctattc agtttcaaaa aacggcatgt 139440

aggcatcact gtaattacct atctatgcaa gtagttaaag agatagaaga atttggtact 139500

atcaatgaaa aaaatttgga atttgacact tggaaagacg ttatacaaaa cgatgaaata 139560

gatgcattag tattttatag agtaaaacaa attagtattt ctacgggtgt tctatataaa 139620

tctatgatgc gcaatagaac aaaacctatt tccatgtact ttgtacgtga ttgtctggca 139680

tttgatggag atcctccgtc ttttagaatg acgtcttgca atatcaacgc atacaatcgt 139740

agtaagatta aagatttaat aatcctaatg aatatgaaaa catgtaataa aaaaattatc 139800

ggtgagttta taatagacaa ttttggaagc gtcgatgcat tactatcgat agttaattcc 139860

aatgttacgt ggattacatc agttataaat aatagtaacg gcaggggtat taatatcagg 139920

gtatcaaata ataaaatgtt aactataact agttttcgac gattcgtcaa taaacttaaa 139980

atgtacaaaa ctactaaatg cgcttctcaa ttggataatc tatgtaccga gatgaacaaa 140040

atggatatta tagacaaaaa atgaaacgta atgaggagta ttgcggggct acataaatta 140100

aaaatggaaa tttttaatgt agaagaattg ataaatatga aaccttttaa gaatatgaat 140160

aaaataacaa ttaatcaaaa tgataattgt atattagcaa atcgatgctt tgttaaaata 140220

gatactccta gatacatacc atcgacatcc attagcagtt ctaatatcat cagaatacgg 140280

aatcatgatt ttacattatc tgaattattg tattcaccgt ttcattttca acagcctcag 140340

tttcaatatc tccttcctgg gtttgtatta acgtgtattg ataaagtttc gaaacagcaa 140400

aaaaaatgta aatattgtat ctctaatcgt ggagatgatg atagtttaag cattaatcta 140460

tttattccga ctattaacaa gtctatatat attattatcg gtttacggat gaaaaatttt 140520

tggaagccta aattcgaaat agaataatgt ttttatatta tacatgttct aaaagaataa 140580

tcgatacagt ttaagtgaaa gctagagagg ggtttttaaa tggtcatcgg tttagtcata 140640

ttcgtgtctg tggcggccgc catcgtcggt gtgttgtcta acgtattgga catgtttatg 140700

tacgtagaag aaaataatga agaggatgct agaatcaagg aggagcaaga actactgttg 140760

ctatattgat acataattga aaatctacca acttaaatac accgcctata aatttacaat 140820

gaaacacaga ttgtattctg aaggattgag tattagtaat gatttaaact cgataatcgg 140880

tcaacaatct acaatggata cggatataga aatagacgaa gatgacatca tggaacttct 140940

taatatattg actgagttag gttgtgatgt cgactttgat gaaaatttta gcgatatagc 141000

cgatgatatt ctagaatcgt tgatagaaca ggatgtataa gtttttatgt taactaaatg 141060

tggccatttg caccggtacc tgcgggagca aaatgtaggc tggtagaaac actaccagaa 141120

aatatggatt ttagatccga tcatttaaca acatttgaat gttttaacga aattatcact 141180

ctagctaaga aatatatata catagcatct ttttgttgta atcctctgag tacgactagg 141240

ggagcgctta tttttgataa actaaaagag gcatctgaaa aagggattaa aataatagtt 141300

ttgctagatg aacgagggaa aagaaatctg ggagagctac aaagtcactg cccggatata 141360

aattttataa ccgttaatat agataaaaaa aataatgtgg gactactact cggttgtttt 141420

tgggtgtcag ataatgaaag atgttatgta ggaaacgcgt catttactgg aggatctata 141480

catacgatta aaacgttagg tgtatattct gattatcccc cgctggccac agatcttcgt 141540

agaagatttg atacttttaa agcctttaat agcgcaaaaa attcatggtt gaatttatgc 141600

tctgcggctt gttgtctgcc agttagcact gcgtatcata ttaagaatcc tataggtgga 141660

gtgttcttta ctgattctcc ggaacaccta ttgggatatt ctagagatct agacactgat 141720

gtagttattg ataaactcaa gtcggctaag actagtatag atattgaaca tttggccata 141780

gttcccacta cacgtgtcga cggtaatagc tactattggc ccgacattta caactccatt 141840

atagaagcag ccattaatag aggagttaag atcagacttc tagttggtaa ttgggataag 141900

aacgacgtat attctatggc aaccgccaga agtctagacg cgttgtgtgt tcaaaatgat 141960

ctatctgtga aggttttcac tattcagaat aatacaaaat tgttgatagt cgacgacgaa 142020

tatgttcata tcacttcggc aaatttcgac ggaacccatt accaaaatca cggattcgtc 142080

agttttaata gtatagataa acagcttgta agcgaggcta aaaaaatatt tgagagagat 142140

tgggtatcta gccacagtaa atcgttaaaa atttaaaaaa tagaaacgta tagaacgcca 142200

tcatgttaaa caggatacaa accttgatga aaacagctaa caattatgaa actattgaga 142260

tattgcgtaa ctatttaaga ctgtatatca ttttggcacg aaatgaagaa ggtcatggta 142320

tactaatata cgatgataac atagatagtg ttatgtcgat gatgaatatt acaatattag 142380

aagttatagg attgacgact cattgcacaa aattaagatc atcgcctcca attcctatgt 142440

ctagattgtt tatggacgaa atagatcatg agtcatatta ttctccaaaa acttcagatt 142500

atccgttgat cgatattata cgaaagcgtt ctcacgaaca gggagatata gcactggctt 142560

tagaacgata cggtattgag aatacagatt ccatatcaga aattaatgaa tggttgtcgt 142620

caaaaggttt agcatgttat agatttgtaa aatttaacga ttataggaaa cagatgtatc 142680

gtaagttctc taggtgtact atagttgaca gtatgataat agggcatata ggtcatcatt 142740

atatttggat taaaaattta gaaacatata cgcgtcccga aattgatgtg ttaccgtttg 142800

atattaaata catatctaga gatgaattgt gggcgcgaat ttcttcctcg ttagatcaaa 142860

cacatataaa aaccatcgcc gtatcagttt atggagctat tactgataat ggaccaatac 142920

catatatgat atccacgtat ccgggtaata cctttgttaa ctttaacagt gtaaaaaatc 142980

taattttaaa tttcttagat tggattaaag atattatgac tagtacacga actatcattc 143040

tagtaggtta catga 143055

<210> 70

<211> 31690

<212> DNA

<213> 人工序列

<220>

<223> 病毒构建体

<400> 70

tggctggaat cattaacaag aaaataaaaa acaatttcaa agttaaacat aactggtatt 60

acgatttcca tttctttgtt catactttat taaaaacata tccagaaatc gaaaaagata 120

tcgaatttag tacggcattg gaagaattca tcatgtgtac caaaacagac tgtgataaat 180

atagattaaa ggtttccatt cttcacccaa ttagtttctt ggaaaaattt attatgagag 240

acattttctc agactggata aatggcggaa actaaagagt ttaaaacttt gtataatctt 300

tttatagata gttatttaca aaaattagct caacattcta tccctactaa tgtcacttgt 360

gctattcata taggagaggt tataggacag tttaaaaatt gcgcgctccg aataactaac 420

aaatgcatga gtaattctcg acttagtttc acactcatgg ttgaatcatt tattgaagtg 480

atttcattgc ttccggaaaa ggatagaaga gctatcgctg aggaaatagg aatagatcta 540

gacgatgtac ctagtgcggt atccaagcta gaaaagaact gtaatgcgta tgcggaggtt 600

aataatatta tagatataca gaaattggat atcggagaat gttcggctcc gcccggtcaa 660

catatgcttt tacagatagt taatacagga tccgcggaag caaattgtgg tttacagaca 720

attgttaagt ccttaaataa aatatacgtt ccacctatta tcgaaaaccg attgccgtat 780

tacgatccgt ggtttctagt gggtgtagca attattctag ttatttttac tgtagctatt 840

tgttctatta gacgaaatct ggctcttaaa tacagatacg gaacgttttt atacgtttaa 900

ttaataaaaa aatttaatta caaggtataa aatagtactc catctacgca atcgcgataa 960

tggagggatc taaacgcaga cacgacagtc ggcgactaca acaagaacag gagcagcctc 1020

gtccacgtac accgccatca tatgaagaaa ttgcaaaata tggacactca tttaacgtga 1080

aaagatttac gaatgaagaa atgtgtctta agaatgatta tccacgaatt atatcatata 1140

atcctccacc aaaatagagt atatatatat catcatttca tgatgtatac tactgacata 1200

gtttcaatgt gaacttttca ctttcttgcc ggttatgaag aatattttta ttttaatggt 1260

cattactaat cgtatattat aattgaaaat ggattagttt aatatgacgc tcgtcatggg 1320

atcctgctgt ggtagattct gtgacgctaa gaataagaat aagaaggaag atgtagaaga 1380

gggaagagaa ggatgttaca attataagaa ccttaatgat ctggatgaat ccgaagcacg 1440

tgtagaattt ggaccattat atatgataaa tgaagaaaaa tcagacataa atacattgga 1500

tataaaaaga agatatagac acacgataga gtctgtatat ttctaaaagt ttttataaaa 1560

aatgagtaaa atactcacgt ttgttaaaaa taagataatt gacttgatta ataatgacca 1620

aattaaatat tctagagtta taatgataga agagtccgat agtcttttac cggttgatga 1680

ggtgcatgct aaccacggat ttgactgtgt ggagatgata gatgaaaata taagcaatga 1740

gaatatcgaa cagtataaaa ccgaatcttt ttttacaata aattgaaatc aaaacattta 1800

ttaaaccgca tcaagatggg tacgaacggc gttagagtat ttgtcatttt atatttgttg 1860

gctgtatgcg gatgtatcga atacgacgta gacgataatg tacatatttg tacccacact 1920

aacgtgtcac atattaatca cactagttgg tattataatg ataaggttat agcgctagcc 1980

accgaggata aaacttctgg ttatatatca tcattcataa aacgcgttaa tatctcatta 2040

acttgtttaa atatatcgag tttgaggtac gaagattctg gtacatacaa aggagtatcg 2100

catctaaaag atggagtcat cgttacgact actatgaata tatctgtaaa ggctaatatc 2160

attgacttga ctggtagagt gcgttatcta accagaaatt attgcgaagt taaaatacga 2220

tgcgaaataa catctttcgc gcttaatggt tctactacac caccacatat gatattagga 2280

acagtagata aatggaaata tcttccattt cctacagatg attatagata cgtaggggaa 2340

ctgaaaagat atatatctgg aaacccatat ccaacagagt cgctagcgtt agaaattagc 2400

tcgacgttta atcggtttac tatcgttaaa aatttgaacg atgacgagtt ttcttgttat 2460

ctgtttccat aaaatgttga acgcgcgcca tatttgtgaa tccgaatggg aggctttaaa 2520

taataataac gataattcat cctccatgcc cgcttcccac aacaatctcg caaacgattt 2580

atctagtatg atgtcacaat tacaaaatga taataacgat aatttaatta tgatagtact 2640

aataacaatg ctatcaataa tacttgtaat tattgtagtg attgcggcga tatcgatgta 2700

caaaagatcc aagtacaggc atatagataa ctgaaaaaaa atttattgtt attgttaatt 2760

tagttatgga acccatcctt gcaccaaatc caaatagatt tgttattttc ccaatccaat 2820

atcatgacat ctggaacatg tataaaaagg cagaggcatc attttggaca gtggaagaag 2880

tagatatatc taaagatatc aatgattgga ataaactaac accagacgaa aaatatttta 2940

taaaacatgt attggcgttt tttgcagcca gtgacggaat agtgaatgaa aatttggcgg 3000

aacgattttg tacagaagta cagattaccg aggctagatg tttctacgga tttcagatgg 3060

ccattgaaaa cattcattcg gaaatgtata gtcttttgat cgatacttat gttaaagata 3120

gtaatgaaaa aaactatctc tttaatgcca tagaaacgat gccttgtgta aaaaagaagg 3180

ccgattgggc tcaaaagtgg atacatgaca gcgccggtta tggagagaga cttattgcct 3240

ttgctgcagt agaaggaatc ttcttttccg gatcattcgc ttccatattt tggcttaaaa 3300

agcgtggcct aatgcccgga ctcacgtttt ccaacgaatt gattagtaga gacgagggtc 3360

tacactgcga cttcgcatgt ttgatgttta aacatttatt gcatccaccg agtgaagaaa 3420

ccgttagatc tattataaca gatgcggtat ccattgaaca agaatttctt actgcggctc 3480

ttccagttaa acttatagga atgaattgtg aaatgatgaa aacatatata gaattcgtcg 3540

cggatagatt gatttctgaa ttgggattta aaaaaattta taatgttacc aatccgtttg 3600

atttcatgga aaatatatca ttagaaggta aaactaattt tttcgaaaaa cgtgtgggtg 3660

aataccaaaa aatgggagtt atgtctcaag aagataatca tttttcttta gatgttgact 3720

tttaaagaaa cataaatgcc gatatttgtc aatactgtgt actgtaagaa tatattagca 3780

ttgtctatga ctaagaaatt caaaacaatt attgatgcca taggaggcaa tataatagtc 3840

aattctacga tattgaaaaa gttatctcct tactttcgca cacatttacg tcaaaaatac 3900

acgaaaaata aagatccagt tacgagggtt tgtctagacc ttgacattca cagtctaact 3960

tctatagtta tttactcata tactggaaag gtatatatag atagtcataa cgtcgtcaat 4020

ttattacgtg cttctatatt aacctctgta gaatttatca tctacacttg tataaacttt 4080

atcctacgag attttagaaa ggaatattgt gtcgagtgtt acatgatggg tatagaatac 4140

ggactatcca atctcttatg tcatactaaa aactttattg ccaaacactt tttggaactg 4200

gaagatgaca tcatagacaa ttttgattat ctatctatga aacttattct agaaagcgat 4260

gaactaaatg ttccagatga ggattttgtc attaagtggt atataaagcg aagaaataaa 4320

ttaggaaatc tgctactcct tatcaaaaat gtaatcaggt caaattatct ttctcccaga 4380

ggtataaata atgtaaaatg gatactagac tgtaccaaaa tatttcattg tgataaacaa 4440

ccacgcaaat catacaagta tccattcata gagtatccta tgaacatgga tcaaattata 4500

gatatattcc atatgtgtac aagtactcat gttggagaag tagtatatct catcggtgga 4560

tggatgaaca atgaaataca taacaatgct atagctgtaa attatatatc aaacaattgg 4620

attccaattc ctccgatgaa tagccccaga ctgtatgcta gcgggatacc cgctaacaat 4680

aaattatacg tagtaggagg tctaccaaat cccacatctg ttgagcgttg gttccacggg 4740

gatgctgctt gggttaatat gccgagtctt ctgaaaccta gatgtaatcc agcagtggca 4800

tccataaaca atgttatata cgtaatggga ggacattctg aaactgatac aactacagaa 4860

tatttgctac ccaatcatga tcagtggcag tttggaccat ccacttatta tcctcattat 4920

aaatcatgcg cgttagtgtt cggtagaagg ttattcttgg ttggtagaaa tgcggaattt 4980

tattgtgaat ccagcaatac atggactctg atagatgatc ctatttatcc gagggataat 5040

ccagaattga tcatagtgga taataaactg ctattgatag gaggatttta tcgtgaatcg 5100

tatatagata ctatagaagt gtacaatcat cacacttatt catggaatat atgggatggt 5160

aaataatttt gaaataaaat attagtttta tgttcaacat gaatattaac tcaccagtta 5220

gatttgttaa ggaaactaac agagctaaat ctcctactag gcaatcgccg ggtgctgccg 5280

gatatgattt gtatagcgct tacgattata ctatccctcc aggagaaaga cagttaatta 5340

agacagatat tagtatgtcc atgcctaaga tttgctatgg tagaatagct cctaggtctg 5400

gtctgtcact aaaaggcatt gatataggag gtggtgtaat agacgaagat tataggggaa 5460

acataggagt cattcttatt aataatggaa aatgtacgtt taatgtaaat actggagata 5520

gaatagctca gctaatctat caacgtatat attatccaga actggaagaa gtacaatctc 5580

tagatagtac aaatagagga gatcaagggt ttggatcaac aggacttaga taataaacaa 5640

tagtatgttg tcgatgttta tgtgtaataa tatcgtagat tatgtagatg gtatagtaca 5700

ggatatagaa gatgaggcta gcaataatgt tgatcacgac tatgtatatc cacttccaga 5760

aaatatggta tatagatttg acaagtccac taacatactc gattatctat caacggaacg 5820

ggaccatgta atgatggctg ttcgatacta tatgagtaaa caacgtttag acgacttgta 5880

tagacagttg cccacaaaga ctagatcata tatagatatt atcaacatat attgtgataa 5940

agttagtaat gattataata gggacatgaa tatcatgtat gatatggcat ctacaaaatc 6000

atttacagtt tatgacataa ataacgaagt taatactata ctaatggata acaaggggtt 6060

gggtgtaaga ttggcgacaa tttcattcat aaccgaattg ggtagacgat gtatgaatcc 6120

agtaaaaact ataaaaatgt ttactctact atcgcatact atatgcgatg attgttttgt 6180

agattatata acggacattt caccaccaga taataccatc cctaacacta gcacgcgtga 6240

atatctaaag cttattggca tcacagctat catgtttgct acatataaaa ctctcaaata 6300

catgatagga taattttttt taacacggat atagaatgct aacgtaataa ttatgcgtta 6360

tgaagacccc tatatcatca attcaatttt ttttctagaa aaagtatcaa gatgtttata 6420

ttatcgtcgg tcatcagatc tgtaatgttt cccaatgatt ggaatctaga ttctgaaatc 6480

tttttgtatc cccaatgttc agtgatttta gcacatattc ctatggtagc gaataaactt 6540

tcctgatcat ggacttttgt aaaattgatg tagtggtctc cttcgctcat agcttcgaca 6600

atctcattaa ttttatcaat accatagtac cgtatagcga catcatcgaa cttcatcaat 6660

tccttgtaca gtcttccaca actggtaata tctttgttaa acactataac atgatttctc 6720

cacgtaatat attcatctat tagatcgatg atggagtcgc gactacatat tttatcatca 6780

tccacaaagt aaaaaacagc atcctcataa tctaatttag tcgccatgac tatctcacaa 6840

aagacagtag ccgtctcctt cctctatatt gatttaattg tatgttttta caattatcaa 6900

taaaacataa aaataatatg atcatcaaac gaactgttaa tattgatagt tatataacgt 6960

gaatcatgag tgcaaactgt atgttcaatc tggacaatga ttacatatcc taaggcatta 7020

gtattcataa gtcatggagc tggtaaacat tctggacgtt atgacgaact agctgaaaac 7080

atatcatcgt taggaatttt agtattctca catgatcata ttggacatgg aagaagtaat 7140

ggtgaaaaaa tgatgattga tgactttggt acagcacgtg gtaactatta aatctactta 7200

ttgggtcatt ccatgggagc aacaatttct atactagcct cttacgataa tccaaacttg 7260

tttacagcaa tgattctaat gtctcctcta gttaatgcag atgctgtttc aagactgaat 7320

ctgctagctg ccaaacttat gggaaccatc acaccaaatg cgccagtcgg aaagctatgt 7380

ccagaatcag tatctagaga tatggataaa gtttataaat accaatacga cccattaatc 7440

aatcatgaaa aaattaaggc tggatttgct agtcaggtct tgaaggctac caacaaggtt 7500

agaaaaataa tttccaagat taacaccccg actctcatac tccagggaac aaacaatgag 7560

attagcgatg ttttaggtgc atattatttc atgcaacatg caaattgtaa tagagaaata 7620

aaaatttatg aaggtgccaa acatcatctt cataaggaaa cggatgaagt taaaaaatca 7680

gtcatgaaag aaatagaaac ttggattttt aatagagtga agtgatatag gattattctt 7740

ttaacaaata aaatgaatcc ggataataca atcgcagtga ttacagagac tattcctata 7800

ggtatgcaat ttgataaagt atatttgtct acatttaaca tgtggaggga aattctatcc 7860

aataccacaa aaacactaga tatatcatct ttttattgga gtttatcgga tgaagtgggt 7920

acgaatttcg gcacgataat attaaacgag attgtacaat tacccaaaag aggagtacga 7980

gttagagtag ccgtcaataa atctaacaaa ccattaaagg atgttgaaag actacaaatg 8040

gccggagttg aagtacgata catagatatt acaaatatcc taggaggagt tcttcataca 8100

aaattttgga tatctgataa tacacatatt tatttaggaa gcgctaacat ggattggaga 8160

tcactaactc aggtcaaaga attgggtatt gcgatcttca ataataggaa cttggcagcg 8220

gatctcactc aaatttttga ggtatactgg tatcttggag ttaacaatct accatataat 8280

tggaaaaact tttatccgtc gtattataat acagatcatc ctcttagtat taacgtaagt 8340

ggtgttccac actctgtatt tattgcttct gcaccgcaac aactatgtac tatggaaaga 8400

accaatgatt taaccgcttt attgtcatgt attagaaatg cgagtaaatt cgtttatgta 8460

tctgttatga actttatccc tattatttat tcgaaggcgg gtaaaatttt gttttggcct 8520

tatatagaag atgaattaag aagatccgct atagacagac aagtatccgt taagctattg 8580

attagttgct ggcaacgatc ttcgtttatc atgagaaact ttttaagatc tatcgctatg 8640

ctaaaatcta aaaacataaa tatagaagta aagctattta ttgtaccaga tgctgatcct 8700

cccattccgt attctagggt aaaccatgcc aaatatatgg taaccgataa aacggcctat 8760

ataggtacct caaattggac aggaaattac tttacggata catgtggagc atctattaat 8820

attacaccgg atgatggatt aggtcttcgt caacaattag aagatatttt tatgcgtgat 8880

tggaattcaa aatacagcta tgaattgtac gatactagtc ctactaaaag gtgtaaacta 8940

ttaaaaaata tgaaacaatg tacaaatgat atatactgcg atgagataca accggaaaaa 9000

gaaattcctg aatattctct tgaataaaat agatataaaa acataatttt tatcccaatt 9060

tacgagcccg ttaacaagat gcttgcattt tgttattcgt tgcccaatgc gggcgatgta 9120

ataaagggca gagtatacga gaatgattat gctctatata tttatctttt tgactatcct 9180

cactctgaag ctatcttggc agagagtgtt aagatgcata tggatagata tgttgaatat 9240

agggataaac tggtagggaa aactgtaaaa gttaaagtga ttagagttga ttatacaaaa 9300

ggatatatag atgtcaatta caaaaggatg tgtagacatc aataattttt ataccgaaca 9360

taaaaataag gttaattatt aataccataa aatcatgatt gcgttattga tactatcgtt 9420

aacgtgttca gcgtctacct atcgtctaca aggatttacc aatgccggta tagtagcgta 9480

taaaaatatt caagatgata atattgtctt ctcaccgttt ggttattcgt tttctatgtt 9540

tatgtcgcta ttgcctgcat caggtaatac tagaatagaa ttattgaaga ctatggattt 9600

gagaaaaaga gatctgggtc cagcatttac agaattaata tcaggattag ctaagctgaa 9660

aacatctaaa tatacgtaca ctgatctaac ttatcaaagt ttcgtagata atactgtgtg 9720

tattaaaccg tcgtattatc aacaatatca tagattcggc ctatatagat taaactttag 9780

acgagatgcg gttaataaaa ttaattctat agtagaacgt agatccggta tgtctaatgt 9840

agtagattct aatatgctcg acaataatac tctatgggca atcattaata ctatatattt 9900

taaaggtata tggcaatatc cgtttgatat cactaaaaca cgcaatgcta gttttactaa 9960

taagtacggt acgaaaacgg ttcccatgat gaacgtagtt actaaattgc aaggaaatac 10020

aatcacaatc gatgacaaag aatatgacat ggtacgcctt ccgtataagg atgctaatat 10080

tagtatgtac ctggcaatag gtgataatat gacccatttc acagattcta ttacggctgc 10140

aaaattagac tattggtcgt ttcaattagg gaataaagtg tacaatctta aactccctaa 10200

attttctatc gaaaataaga gggatattaa gtcgatagcc gaaatgatgg ctcctagtat 10260

gtttaatcca gataatgcgt cgtttaaaca tatgactagg gacccattat atatttataa 10320

aatgtttcag aatgcaaaga tagatgtcga cgaacaagga actgtagcag aggcatctac 10380

tattatggta gctacggcga gatcatctcc tgaaaaactg gaatttaata caccatttgt 10440

gttcatcatc agacatgata ttactggatt tatattgttt atgggtaagg tagaatctcc 10500

ttaatatggg tacggtgtaa ggaatcatta ttttatttat attgatgggt acgtgaaatc 10560

tgaattttct taataaatat tatttttatt aaatgtgtat atgttgtttt gcgatagcca 10620

tgtatctact aatcagatct attagagata ttattaattc tggtgcaata tgacaaaaat 10680

tatacactaa ttagcgtctc gtttcagaca tggatctgtc acgaattaat acttggaagt 10740

ctaagcagct gaaaagcttt ctctctagca aagatgcatt taaggcggat gtccatggac 10800

atagtgcctt gtattatgca atagctgata ataacgtgcg tctagtatgt acgttgttga 10860

acgctggagc attgaaaaat cttctagaga atgaatttcc attacatcag gcagccacat 10920

tggaagatac caaaatagta aagattttgc tattcagtgg actggatgat tcgtattcga 10980

tgattatttt taacaaaata acataaaaat aatatatttt tttaggatgc gatcatgacg 11040

tcctctgcaa tggataacaa tgaacctaaa gtactagaaa tggtatatga tgctacaatt 11100

ttacccgaag gtagtagcat ggattgtata aacagacaca tcaatatgtg tatacaacgc 11160

acctatagtt ctagtataat tgccatattg gatagattcc taatgatgaa caaggatgaa 11220

ctaaataata cacagtgtca tataattaaa gaatttatga catacgaaca aatggcgatt 11280

gaccattatg gagaatatgt aaacgctatt ctatatcaaa ttcgtaaaag acctaatcaa 11340

catcacacca ttaatctgtt taaaaaaata aaaagaaccc ggtatgacac ttttaaagtg 11400

gatcccgtag aattcgtaaa aaaagttatc ggatttgtat ctatcttgaa caaatataaa 11460

ccggtttata gttacgtcct gtacgagaac gtcctgtacg atgagttcaa atgtttcatt 11520

gactacgtgg aaactaagta tttctaaaat taatgatgca ttaatttttg tattgattct 11580

caatcctaaa aactaaaata tgaataagta ttaaacatag cggtgtacta attgatttaa 11640

cataaaaaat agttgttaac taatcatgag gactctactt attagatata ttctttggag 11700

aaatgacaac gatcaaacct attataatga tgattttaaa aagcttatgt tgttggatga 11760

attggtagat gacggcgatg tatgtacatt gattaagaac atgagaatga cgctgtccga 11820

cggtccattg ctagatagat tgaatcaacc agttaataat atagaagacg ctaagcgaat 11880

gatcgctatt agtgccaaag tggctagaga cattggtgaa cgttcagaaa ttagatatag 11940

agaaaatagc tccagaatac agacaatgct tacaggatct ataccatatg aaaattacgc 12000

gtcctagaca ctttgataac tagttatgtc attctcgttt ttatagagtg tactcgttac 12060

taaatattaa ttaatgaaat aataacaatt attaagacat actattcatc caacttaaac 12120

aacgaaaaac atttttcatt aagtttatca tgaatgcgta taataaagcc gattcgtttt 12180

ctttagagtc tgattctatc aaagatgtta tacacgatta tatttgttgg ctcagtatga 12240

ctgatgaaat gagaccatct atcggaaacg tctttaaagc gatggaaacg tttaagatag 12300

acgcggttag atattacgat ggtaacatat acgatttagc taaagatata aatgcgatgt 12360

cattcgacag ttttataaga tctctacaaa atatctcttc aaagaaagat aaactcactg 12420

tttatggaac catgggactg ctgtctattg tcgtagatat taacaaaggt tgtgatatat 12480

ccaatatcaa gttcgctgcc ggaataatca ttttaatgga gtatattttt gatgacacgg 12540

atatgtctca tcttaaagta gcactctatc gtagaataca gagacgtgat gatgtagata 12600

gatatttttt tttcctaaac tgatttctct gtttaaattc gtagcgatat ataaaacaac 12660

atgtaattaa ttaataaact ttaagacatg tgtgttatac taagatggtt ggcttattcc 12720

atagtagctt gtggaattta taaacttatg atagtaaaac tagtacccaa tatgtaaaga 12780

tgaaaaagta aattactatt aacgccgtcg gtattcgttc atccattcag tatgggtata 12840

cagcacgaat tcgacatcat tattaatgga gatatcgcgt tgagaaattt acagttacat 12900

aaaggggata actacggatg caaactaaaa attatttcga atgattacaa gaaattaaag 12960

tttagattca ttatacgccc agattggtcg gaaatcgacg aggtcaaagg attaaccgta 13020

tttgcaaaca actatgcggt gaaagttaat aaggtagatg acacgttcta ttacgtaata 13080

tatgaggctg taatacatct gtataacaaa aaaacagaga tattgattta ttctgatgat 13140

gagaacgaac tctttaaaca ctattaccca tacatcagtc taaatatgat tagtaaaaag 13200

tataaagtta aagaagaaaa ctactcatcc ccgtatatag aacatccgtt aatcccgtat 13260

agagattatg agtccatgga ttaatatgag tatagtgtta aatgacactt actaaatagc 13320

caaggtgatt attcgtattt ttttaaggag taaccatgtc cgcaattaga tttattgcat 13380

gtctatatct catttccatc ttcggaaatt gtcatgagga tccatattat caaccatttg 13440

ataaattaaa cattactcta gatatataca cttatgagga tctagtacca tacaccgtag 13500

acaatgacac aacttctttc gttaagatat actttaaaaa tttttggatt acggttatga 13560

ctaaatggtg tgctccgttt attgataccg ttagcgtata cacatctcat gataatctga 13620

atatacaatt ttatagtagg gacgaatatg atacacaaag cgaggataaa atttgtacca 13680

ttgatgttaa agcacgatgc aaacatctaa caaaacgaga agttacagta caacaagaag 13740

cctacagata ttcattatct tctgacctat cgtgttttga ttctatagat ctagagattg 13800

atcttattga aactaatagt actgacacta cagtactgaa atcatatgag ctcatgcttc 13860

ccaaacgtgc taaatccata cataactgaa atgaaagaaa ccaaaaaatg cgatagcatc 13920

aacaaccaat catggttaac gataagatac tctatgatag ttgtaaaaca tttaacatcg 13980

atgccagcag tgcacaatca ttgatagaaa gtggtgcaaa tccattatat gagtatgatg 14040

gtgaaactcc attaaaggca tacgttacca agaaaaataa taatatcaaa aacgatgttg 14100

tgattttgtt attgtcgtca gtcgactata aaaatatcaa tgattttgat atactcgaat 14160

atctatgttc tgataacatc gatatagact tattgaaatt actaatttcg aaaggtatag 14220

aaataaatag tatcaaaaat ggtattaata ttgtagaaaa atacgctaca acatcaaatc 14280

ccaatgtaga tgtgtttaaa ctattattgg ataaaggaat acctacatgt agcaacatac 14340

agtatggata caagatcaaa atagaacaga ttagacgtgc tggtgaatat tataattggg 14400

atgatgaatt agacaattac gattacgact acaccactga ttatgatgat agaatgggta 14460

aaacagttct ctattattat attattacta ggtcacaaga tggttatgct acatctttgg 14520

acgtaataaa ctatttaatt tcacacgaaa aagagatgcg ttattatact tatcgtgaac 14580

ataccacact ctattattat cttgacaaat gcgatattaa acgggaaata tttgacgcgt 14640

tattcgatag taactatagt ggtcatgaac taatgaatat tctatctaac tatttacgta 14700

aacagtttag gaagaaaaat cacaaaatcg ataattatat agttgatcaa ctattattcg 14760

accgtgatac gttttatatt ttagaattgt gtaatagttt acgtaataat atccacaatt 14820

cttaaaagat atacagattc tatacaagat ctattgttag aatatgtatc ttatcataca 14880

gtatacatca atgttattaa atgtatgatt gatgaaggag ctacattata tagatttaag 14940

catataaata aatattttca aaaatttggc aatagagatc ctaaagttgt cgagtatatt 15000

ttaaaaaatg gaaacttagt tgtagataat gacaatgatg ataacctaat aaatattatg 15060

ccattattcc ctaccttctc tatgcgtgag ttggatgtgt tatcgatact aaaactttgt 15120

aagccgtata ttgatgatat aaacatggat gtagtatact ttatcattgt attaagtcgc 15180

atagtgtcag cctagtagaa tggttaatag ataatggcgc agacattaat ataataacaa 15240

aatatgggtt tacatgtatt actatttgtg ttatactggc agataaatat atcccagaaa 15300

tagcagaatt atatattaag atattggaaa ttattctgag taaattacca accatcgaat 15360

gtattaagaa aacagttgat tacctagacg atcacaggta cttattcata ggtggtaata 15420

ataaatcgtt actgaaaata tgtatcaagt acttcatatt agtcgattat aagtacacat 15480

gtagcatgta tccatcatat atagaattta taaccgactg cgaaaaagaa attgcggata 15540

tgcgtcaaat taaaataaat ggtacggaca tgcttacagt gatgtacatg ttaaataaac 15600

ctacaaagaa acgatatgtt aataatccga tatttacaga ttgggctaat aagcaatata 15660

agttttataa tcaaataata tataatgcta ataagttaat agaacaaagt aagaaaatag 15720

acgacatgat agaggaggta tccattgacg ataatcgttt atcaacacta ccgttagaaa 15780

ttagacattt gattttctcg tacgcgttcc tataaaaata gaaactataa tcatataata 15840

gtgtaggttg gtagtattgc tcttgtgact agagacttta gttaaggtac tgtaaaaata 15900

gaaactataa tcatataata gtgtaggttg gtagtagggt actcgtgatt aattttattg 15960

ttaaacttgt ccttaagtct tattaatatg tcttctaaag ggggtagtgg cggcatgtgg 16020

agtgtcttta tccatggaca tgatggtagt aataaaggat ctaaaactta tacatctggt 16080

ggcggtggaa tgtggggagg aggatcgtcc agtggtgtaa aaagtggggt taacggaggt 16140

gtaaaatctg gaactggtaa aatttaaaca ctaaattatt tttattaata attgtacaag 16200

tttttgacat gatatttaat gacattagtt gtgtgggtgt atagagttca cagtagctca 16260

ttcagtcaaa atgtttgact atttggaaaa tgaggaggtg gctctcgatg aacttaaaca 16320

gatgttgaga gacagagatc ctaatgatac caggaaccaa ttcaagaata atgctttaca 16380

cgcatacctt tttaatgagc attgtaataa tgtcgaggtc gtcaaactac tactagacag 16440

tggcactaat ccattacgca aaaattggag acagctaccc cattagaaga atacacaaat 16500

agtagacatg ttaaagttaa aggatatagc gatggctcta ctagaagcca ctggatttag 16560

caacataaat gactttaata tattcagcta tatgaaatcc aaaaatgtag acgttgactt 16620

gataaaggtg ttggtagaac atggatttga cttgagtgtt aaatgtgaaa accatcgttc 16680

agttatagaa aattatgtaa tgacagatga tcctgttcct gaaattattg atttgttcat 16740

agaaaatggc tgcagtgttc tttatgagga cgagtactga tacgcgtatg atgattatca 16800

actacgaaat tgcggtaccg tattgcatct gtatatcatc tctcatctgt attcagagtc 16860

ggatacgaga gcatatgtgc gtccggaagt tgttaaatgt ctaattaatc acggaatcaa 16920

gccgtctttt atagataaaa actattgtac agctcttcaa tattatatta agtcatctca 16980

tatagatata gacatcgtta aattgttaat gaaaggaata gataacacgg cttattcata 17040

tatagacgat ctaacatgtt gcactcgagt aattatggct gattatctaa atagtgatta 17100

tagatacaat aaagatgtag atttggtcaa attgtttttg gaaaatggaa agccgcacgg 17160

aataatgtgt agtattgtac cactatggag aaatgataag gaaaccatct ttttgatatt 17220

gaaaacaatg aactcggatg tcctccaaca tatactaatt gagtatatga cattcggcga 17280

tatccctcta gtggaatatg gaactgtggt aaataaagag gctatacacg gatactttag 17340

aaatattaat attgattctt acacgatgaa atatctacta aaaaaggaag ggagatgcca 17400

tcaattatct cgatgatgga gagatcccga ttggacacct atgtaaatcc aactatgaat 17460

gttataattt ttacacttat acatacaaaa agggtctttg tgacatgtct tatgcttgcc 17520

caattcttag tactataaac atttgcctac cttatcttaa agacattaac atgattgaca 17580

aacgaggaga aacacttctt cacaaggctg ttagatataa taaacaatct ctagtatctt 17640

tactgctaga atccggttca gatgtcaaca ttagatcaaa taacggatat acatgtatag 17700

ccattgccat caacgaatct aaaaacattg aactgctgaa aatgctatta tgtcataaac 17760

ctacattaga ttgtgtgatt gattcattga gagaaatatc taacatcgta gataacgact 17820

atgctataaa acaatgtatt aaatatgcca tgattataga tgactgtaca tcgtctaaga 17880

ttccagagtc cataagtcaa cgctataatg attatataga tctttgcaat taagaattga 17940

atgagatgaa aaaaataatg gtaggtggta atactatgtt ctcattaata tttactgatc 18000

atggagctaa aattattcat agatatgtca ataatccaga attacgtgag tattatgagt 18060

taaaacaaaa taaaatatat gtggaagcat atgatattat ttccaacgca atagtgaaac 18120

atgatagaat acataaaacc atagaatcag ttgatgataa tacctacatt tctaatcttc 18180

cgtataccat caaatacaaa atattcgagc aacaataagt attttttata cctttaaaat 18240

tgataaataa attttttcta gtgatatttt ggcaagatga aaatcctatt tctcatcgct 18300

ttcatgtatg ggtgtgttca ctcatatgtt aacgcggttg aaaccaaatg tccaaatcta 18360

gacattgtaa catcttctgg agaatttcat tgttcaggat gtgtggaaca tatgcctgag 18420

tttagctata tgtattggtt ggcaaaggat accaagttta tagaacatct gggtgatggc 18480

atcaaagaag atgaaaccgt tcgtaccaca gatagtggaa tcaccactct acgtaaagtc 18540

cttcatgtaa ccgatactaa taaatttgct cattataggt tcacttgtgt cctcactacg 18600

atagatggcg tttcaaaaaa gaatatttgg ctgaagtagt gcgtgctact atttttattt 18660

atgatataat ctaatggaat taatttgaat tgatatttat ccaatactaa agattatatt 18720

agaatcaaat taatctttta tacgagaaaa aataacgaca tacgtcgtca acaaattaaa 18780

ctttttattt attagttaac ttgctcattg ttatgtttct aaaacgggta cgacatatag 18840

gacaattatc cgacgcaccg gtttctcttc gtgttctatg ccatatattg atgcatgtta 18900

tgcaaaatat atgattacac gaatccaata aaccaaagta tctatcgttt tgagtaaaca 18960

acttcatagc aaatttcaca ttctttttct ttacttactc tatacacgtc ctcgtattta 19020

tccagtattt tgatgatatc caactcagaa atggttgttg tattattggg tgtataggta 19080

ttattagcta tgtaccaatt taccaacctt cttaatattg attgataatc acatcggtta 19140

tccaattaat aactaaattg tagtgtatat atagaccata tatgtttcta tttttttgac 19200

agtttcagta agttttgatt gttgtattcc tgtatctcta gataagttag tcatatagtc 19260

ccttccggcg atacgttttt tccaagcccg aaattgatta gccaaatgtg gatttatttt 19320

tgtgataatg catactgtta gtcttatatc atttggttca tctatgtatt gtaatattgt 19380

tacatgatct atagatgatg tattgatttt ggcaggatcg aattccatat ccgcgactaa 19440

acagtgaaaa aaatgtaaat aattttaaat tagtaaaact tttttttatt ttttatgatt 19500

ccaaaaaaac tgaatacaaa gtcctaaatt ataaatatgg agatcatact accacaactt 19560

attattatgt atacaaggcc ggtgtaatag atagatatat ataattctat tacaccggca 19620

gacaattacc gatcggtatt tgtcgttacc aacataccgt ataatatgta atatacaatt 19680

ccataaccca ttgacagttg ttatacatca aaattgcaat tcttttgatt acgatgttat 19740

aagaatgtag ttaattgatg tatgatgtta atgtgtcctc tttcctctta taacatcgta 19800

atcaaaaact tttttataat atatacctaa taatgtgtct taatagttct cgtgattcgt 19860

caaacaatca ttcttataaa atataataaa gcaacgtaaa aacacataaa aataagcgta 19920

actaataaga caatggatat ttacgacgat aaaggtctac agactattaa actgtttaat 19980

aatgaatttg attgtataag gaatgacatc agagaattat ttaaacatgt aactgattcc 20040

gatagtatac aacttccgat ggaagacaat tctgatatta tagaaaatat cagaaaaata 20100

ctatatagac gattaaaaaa tgtagaatgt gttgacatcg atagtacaat aacttttatg 20160

aaatacgatc caaatgatga taataagcgt acgtgttcta attgggtacc cttaactaat 20220

aactatatgg aatattgtct agtaatatat ttggaaacac cgatatgtgg aggcaaaata 20280

aaattatacc accctacagg aaatataaag tcggataagg atattatgtt tgcaaagact 20340

ctagacttta aatcaacgaa agtgttaact ggacgtaaaa caattgccgt tctagacata 20400

tccgtttcat ataatagatc aatgactact attcactaca acgacgacgt tgatatagat 20460

atacatactg ataaaaacgg aaaagagtta tgttattgtt atataacaat agatgatcat 20520

tacttggttg atgtggaaac tataggagtt atagtcaata gatctggaaa atgtctgtta 20580

gtaaataacc atctaggtat aggtatcgtt aaagataaac gtataagcga tagttttgga 20640

gatgtatgta tggatacaat atttgacttt tctgaagcac gagagttatt ttcattaact 20700

aatgatgata acaggaatat agcatgggac gatgatacag atatatggac tcccgtcaca 20760

gaagatgatt acaaatttct ttctagacta gtattgtatg caaaatctca atcggatact 20820

gtattcgact attatgttct tactggtgat acggaaccac ccactgtatt cattttcaag 20880

gtaactagat tttactttaa tatgccgaaa taaaaaattt ttgtataata tctagaggta 20940

gaggtattgt ttagataaat acaaataaca tagatacatc gcatatttag catttttata 21000

aatatacata agacatacac tttatacatt tttgtaaaaa tactcataaa aaaaatttat 21060

aaaaattatg gcacaaccat atcttgtata ggtagtttag ttcgtcgagt gaacctataa 21120

acagataata gacaacacgt aataataata atgcctacta atacaagcat aataccggga 21180

gatgggatat atgacgttgt agtgtttggt ttttctgaac gttgatagtc tactaatact 21240

acatgctgac atctaatgcc tgtataacca tgagagcatc tacaatacat accgtcgata 21300

tctctagcgt ggatacagtc accgtgtaaa caatatccat ctccctctgg accgcataat 21360

ctgatagctg gaatatctgt tgtagcgttt gtaatttctg gcgatgtcgt ttcgatagcg 21420

ttaccactat cggcgaatga tctgattatc atagcagcga acaacaacat cagataattt 21480

atcaacattt ttgatggatt ttgtgtttat gctgtttctc agtgtgtgtt tatgacaaga 21540

ttgggaattt tatattatta attcagtaat ataaactaat aatatattgt taattgtgta 21600

aataatataa aaataacaat acaatattga atgtgttgct gttaaaaatg tatgtgttaa 21660

tataatagaa taaaataaat gagtatgatc attttagata acgattgatt ttatcattac 21720

cgcttcattc ttatattctt tgcttacgga acctatattt agaaacatct actaacgatt 21780

ttttatgctt gcattattaa tggtatgtaa taccaatttg ttaagtatga atacggggta 21840

caaacataaa ctgaaattta gatcattaaa tgtttcatca gaaatgactc catgaaaacc 21900

gccgaagaac ttcgtgcaat cattggactt tgtactcaat cagctatcgt ctctggaaga 21960

gtcttcaacg ataagtatat cgacatacta cttatgctgc gaaagattct gaacgagaac 22020

gactatctca ccctcttgga tcatatccgc actgctaaat actaaatctc cttcatgctc 22080

tctcactaca ctttttatca tcttatgagg aataattagc accagaatag ctatggattg 22140

cacatgtatt ctatgtcgtc tactggatga agatgtgacg tacaaaaaaa taaaactaga 22200

aattgaaacg tgtcacaact tatcaaaaca tatagataga cgaggaaaca atgcgctaca 22260

ttgttacgtc tccaataaat gcgatacaga cattaagatt gttcgactgt tactctctcg 22320

cggagtcgag agactttgta gaaacaacga aggattaact ccgctaggag catacagtaa 22380

gcatagatac gtaaaatctc agattgtgca tctactgata tccagctatt cgaattcctc 22440

taacgaactc aagtcgaata taaatgattt cgacttacgt ctgctaaaat acctaattgt 22500

ggataaacgg atacgtccgt ccaagaatac gaattatgca atcaatggtc tcggattggt 22560

ggatatatac gtaacgacgc ctaatccgag accagaagta ttgctatggc ttcttaaatc 22620

agaatgttac agcaccggtt acgtatttcg tacctgtatg tacaacagtg atatgtgtaa 22680

gaactctctt cattactata tatcgtctca tagagaatct ctatccaagg atgtaattaa 22740

atgtttgatc gataacaatg tttccatcca atactactgg tcttgctcaa ccatagatat 22800

agagattatt aataaaggat gtggacacgt gtagagtata cgacgtcagc cctatattag 22860

aggcgtatta tctaaacaag cgatttagag taaccccata taatgtagac atggaaatcg 22920

ttaatcttct tattgagaga cgtcatactc ttgtcgacgt aatgcgtagt attacttcgt 22980

acgattccag agaatataac cactacatca tcgataacat tctaaagaga tttagacaac 23040

aggatgaatc catcgtacaa gccatactga taaactactt acattacggc gatatggtaa 23100

gtatacctat cattcaatgc atgttggata agacgacgga caacaacttt gttaataata 23160

atctcgtcga tgtaaacgtc gtaaggttta tcgtggaaaa tatggacacg cggctgtaaa 23220

tcacatatct aacaatggcc gtctatgtat gtacggtctg atattatcga gatttaataa 23280

ttgcgggtat cactgttatg aagatgtatt tgatatacta agcaagtaca tggatgatat 23340

agatatgatc gataactcta ctatattacg cggtcgatgt caataatata caatttgcaa 23400

agcggttatt ggaatatgga gcgagtgtca cgctcgataa tcaatacggc catccagaaa 23460

agcagttacc aaagagaagc tagttgattt attactgagt taccatccca ctctagagac 23520

tatgattgac gcatttaata gagatatacg ctatctatat cctgaaccat tattcgcctg 23580

tatcagatac gccttaatcc tagatgatga ttttccttct aaagtaagta tgatatcgcc 23640

ggtcgtcata aggaactaaa gcgctataga gcagacatta atagaatgaa gaatgcctac 23700

atatcaggcg tctccatgtt tgatatatta tttaaacgaa gcaaacgcca cagattgaga 23760

tacgcaaaga acaatgagag gatcgactcc attaaataat ttatcatgga gtgataatgt 23820

cctgtttcca tggcatatta caaaatcgat tccgtccaag atgataaaaa catttaccgg 23880

catcataaac acggagttta ttttatatgt ctcgcataaa cattactaaa aaaatatatt 23940

gttcggtttt ctttcacatc tttaattatg aaaaagtaaa tcattatgag atggacgcat 24000

cgttcgcgac agtatgtggt acatacctaa cgtatttatg gacgacggta agaatgaagg 24060

tcacgtttct gtcaacaatg tcgacgcgat cgtgtaacac gactcacaat agaatctgtg 24120

aatgctctcc cgatcatgga tgcaaggcat gtgtttccca aacaaaatgt ggaataggat 24180

acggagtatc cggagacgtc atctgttctc cgtgtggtct cggaacatat tctcacaccg 24240

tctcttccgc agataaatgc gaacccgtac ccagaaatac ctttaactat atcgatgtgg 24300

aaattaacct gtatccagtt aacgacacat cgtgtactcg gacgaccact accggtctca 24360

gcgaatccat ctcaacgtcg gaactaacta ttactatgaa tcataaagac tgcgatcccg 24420

tcttcttaat aaggtagcga cttcaggttt ctttacagga gaaaggtgtg cactctgaat 24480

ttcgagatta aatgcaataa caaagattct tcctccaaac agttaacgaa agcaaagaat 24540

gatactatca tgccgcattc ggagacagta actctagcgt cgacatctat atactatata 24600

gtaataccaa tactcaagac tacgaaactg atacaatctc ttatcatgtg ggtaatgtag 24660

ccatatgccc ggtagttgcg atatacataa actgatcact aattccaaac ccacccgctt 24720

tttatagtaa gtttttcacc cataaataca ataattaatt tctcgtaaaa gtagaaaata 24780

tattctaatt tattgcacgg taaggaagta gaatcataaa gaacagtact caatcaatag 24840

caatcatgaa acaatatatc gtactggcat gcatgtgcct gccagtcttc agcaatcatc 24900

ctcatcgtgt acggaagaag aaaacaaaca tcatatggga atcgatgtta ttatcaaagt 24960

cacaaagcaa gaccaaacac cgaccgatga taagatttgc caatccgtaa cggaaattac 25020

agagtccgag tcagatccag atcccgaggt ggaatcagtc gaggatgtag atcctcctac 25080

cacttattac tccatcatcg gtggaggtct gagaatgaac tttggattca ccaaatgtcc 25140

tcagattaaa tccatctcag aatccgctga tggaaagact gtgaggtgtc tatcgacatc 25200

agatgtagcg aagaagagaa agacagcgac atcaagaccc atccagtact cgggtctaac 25260

atctctcata agaaagtgag ttacgaagat atcatcggtt caacgatcgt cgatacaaaa 25320

tgtgtcaaga atctagagtt tagcgttcgt atcggagaca tgtgcaagga atcatctgaa 25380

cttgaggtca agtatgtcga cggatcggca tctgaaggtg caaccgatga tacttcactc 25440

atcgattcaa caaaactcaa agcgtgtgtc tgaatcgata actctattca tctgaaattg 25500

gatgagtagg gttaatcgaa cgattcaggc acaccacgaa ttaaaaaagt gtaccggaca 25560

ctatattccg gtttgcaaaa caaaaagtta cctctcgcga cttcttcttt ttctgtctca 25620

atagtgtgat acgattatga cactattcct atttcctttc agggtatcac aaaaatatta 25680

aacctctttc tgatggtctc atacaaaaat atttttattc tctttctctc tttgatggtc 25740

tcataaaaaa tatttttatt ctctttctct ctttgatggt ctcataaaat atttttattc 25800

tctttctctc tttgatggtc tcataaaaaa tatttttatt ctctttctct ctttgatggt 25860

ctcataaaat atttttattc tctttctctc tttgatggtc tcataaaaaa tatttttatt 25920

ctctttctct ctttgatggt ctcataaaaa atattaaacc tctttctgat ggtgtcacta 25980

aaatattttt attctctttc tctcttcaat ggagtcataa aatattttta ttctctttct 26040

ctcttcgatg gtctcacaaa aatattaaac ctctttctga tggtgtcact aaaatatttt 26100

tattctcttt ctctcttcaa tggagtcata aaatattttt attctctttc tctctttgat 26160

ggtctcacaa aaatattttt attctctttc tctctttgat ggtctcacaa aaatattttt 26220

attctctttc tctctttgat ggtctcacaa aaatattttt attctctttc tctctttgat 26280

ggtctcataa aaaaagtttt acaaaaatat ttttattctc tttctctctt tgatggtctc 26340

ataaaaaaag ttttacaaaa atatttttat tctctttctc tctttgatgg tctcataaaa 26400

aaagttttac aaaaatattt ttattctctt tctctctttg atggtctcat aaaaaatatt 26460

aaacctcttt ctgatggtgt cactaaaata tttttattct cattctctct tcaatggagt 26520

cataaaatat ttttattctc tttctctctt cgatggtctc acaaaaatat taaacctctt 26580

tctgatggtg tcactaaaat atttttattc tcattctctc ttcaatggag tcataaaata 26640

tttttattct ctttctctct tcgatggtct cacaaaaata ttaaacctct ttctgatggt 26700

gtcactaaaa tatttttatt ctcattctct cttcaatgga gtcataaaat atttttattc 26760

tctttctctc tttgatggtc tcataaaaaa agttttacaa aaatattttt attctctttc 26820

tctctttgat ggtctcataa aaaatattaa acctctttct gatggtgtca ctaaaatatt 26880

tttattctct ttctctcttc aatggagtca taaaatattt ttattctctt tctctcttcg 26940

atggtctcac aaaaatatta aacctctttc tgatggtgtc actaaaatat ttttattctc 27000

attctctctt caatggagtc ataaaatatt tttattctct ttctctcttt gatggtctca 27060

taaaaaaagt tttacaaaaa tatttttatt ctctttctct ctttgatggt ctcataaaaa 27120

aagttttaca aaaatatttt tattctcttt ctctctttga tggtctcata aaaaaagttt 27180

tacaaaaata tttttattct ctttctctct ttgatggtct cataaaaaaa gttttacaaa 27240

aatattttta ttctctttct ctctttgatg gtctcataaa aaatattaaa cctctttctg 27300

atggtgtcac taaaatattt ttattctcat tctctcttca atggagtcat aaaatatttt 27360

tattctcttt ctctcttcga tggtctcaca aaaatattaa acctctttct gatggtgtca 27420

ctaaaatatt tttattctca ttctctcttc aatggagtca taaaatattt ttattctctt 27480

tctctctttg atggtctcat aaaaaaagtt ttacaaaaat atttttattc tctttctctc 27540

tttgatggtc tcataaaaaa agttttacaa aaatattttt attctctttc tctctttgat 27600

ggtctcataa aaaaagtttt acaaaaatat ttttattctc tttctctctt tgatggtctc 27660

ataaaaaaag ttttacaaaa atatttttat tctctttctc tctttgatgg tctcacaaaa 27720

atattaaacc tctttctgat ggagtcgtaa aaaagtttta tctctttctc cttcgatggt 27780

ctcacaaaaa tattaaacct ctttctgatg gagtcgtaaa aaagttttat ctctttctct 27840

cttcgatggt ctcacaaaaa tattaaacct ctttctgatg gagtcgtaaa aaagttttat 27900

ctctttctct cttcgatggt ctcactaaaa tattttttat tctctttctg atgcatcaac 27960

tatttcttaa acaataacgt ccaacaacat atactcgtcg agcttatcaa catcccctat 28020

gcccatctag gttaccagac aattgtatat cataaaataa tgtttataat ttttacaaaa 28080

atatttttat tctctttctc tctttgatgg tctcataaaa aaagttttac aaaaatattt 28140

ttattctctt tctctctttg atggtctcat aaaaaatatt aaacctcttt ctgatggtgt 28200

cactaaaata tttttattct cattctctct tcaatggagt cataaaatat ttttattctc 28260

tttctctctt cgatggtctc acaaaaatat taaacctctt tctgatggtg tcactaaaat 28320

atttttattc tcattctctc ttcaatggag tcataaaata tttttattct ctttctctct 28380

ttgatggtct cataaaaaaa gttttacaaa aatattttta ttctctttct ctctttgatg 28440

gtctcataaa aaaagtttta caaaaatatt tttattctct ttctctcttt gatggtctca 28500

taaaaaaagt tttacaaaaa tatttttatt ctctttctct ctttgatggt ctcataaaaa 28560

aagttttaca aaaatatttt tattctcttt ctctctttga tggtctcata aaaaaagttt 28620

tacaaaaata tttttattct ctttctctct ttgatggtct cataaaaaaa gttttacaaa 28680

aatattttta ttctctttct ctctttgatg gtctcataaa aaaagtttta caaaaatatt 28740

tttattctct ttctctcttt gatggtctca taaaaaatat taaacctctt tctgatggtg 28800

tcactaaaat atttttattc tctttctctc ttcaatggag tcataaaata tttttattct 28860

ctttctctct tcgatggtct cacaaaaata ttaaacctct ttctgatggt gtcactaaaa 28920

tatttttatt ctcattctct cttcaatgga gtcataaaat atttttattc tctttctctc 28980

tttgatggtc tcataaaaaa agttttacaa aaatattttt attctctttc tctctttgat 29040

ggtctcataa aaaaagtttt acaaaaatat ttttattctc tttctctctt tgatggtctc 29100

ataaaaaaag ttttacaaaa atatttttat tctctttctc tctttgatgg tctcataaaa 29160

aaagttttac aaaaatattt ttattctctt tctctctttg atggtctcat aaaaaaagtt 29220

ttacaaaaat atttttattc tctttctctc tttgatggtc tcataaaaaa tattaaacct 29280

ctttctgatg gtgtcactaa aatattttta ttctctttct ctcttcaatg gagtcataaa 29340

atatttttat tctctttctc tcttcgatgg tctcacaaaa atattaaacc tctttctgat 29400

ggtgtcacta aaatattttt attctctttc tctcttcaat ggagtcataa aatattttta 29460

ttctctttct ctctttgatg gtctcataaa aaaagtttta caaaaatatt tttattctct 29520

ttctctcttt gatggtctca taaaaaaagt tttacaaaaa tatttttatt ctctttctct 29580

ctttgatggt ctcataaaaa aagttttaca aaaatatttt tattctcttt ctctctttga 29640

tggtctcata aaaaaagttt tacaaaaata tttttattct ctttctctct ttgatggtct 29700

cataaaaaaa gttttacaaa aatattttta ttctctttct ctctttgatg gtctcataaa 29760

aaaagtttta caaaaatatt tttattctct ttctctcttt gatggtctca taaaaaaagt 29820

tttacaaaaa tatttttatt ctctttctct ctttgatggt ctcataaaaa atattaaacc 29880

tctttctgat ggtgtcacta aaatattttt attctcattt tctctttctc tcttcaatgg 29940

agtcataaaa tatttttatt ctctttctct ctttgatggt ctcataaaaa atattaaacc 30000

tctttctgat ggtgtcacta aaatattttt attctcattc tctcttcaat ggagtcataa 30060

aaaagtttta tctctttctc tcttcgatgg tctcacaaaa atattaaacc tctttctgat 30120

ggagtcgtaa aaaagtttta tctctttctc tcttcgatgg tctcacaaaa atattaaacc 30180

tctttctgat gcatcaacta tttcttaaac aataacgtcc aacaacatat actcatcccc 30240

tatgcccatc taggttacca gacaattgta tatcataaaa taatgtttat aatttacacg 30300

ttaaaatcat ataataaaac gtagatcgta taatattttt tggtatataa atgatctagt 30360

aaaatccatg taggggatac tgctcacatt ttttctttgg tacaaaattt cacacaagtt 30420

tttatacaga caaattcttg tccatatatt ttaaaacatt gacttttgta ctaagaaaaa 30480

tatctagact aactatctct ttctctttct ctcttcgatg gtctttctga tggagtcgta 30540

aaaaagtttt atctctttct ctcttcgatg gtctcacaaa aatattaaac ctctttctga 30600

tggagtcgta aaaaagtttt atctctttct ctcttcgatg gtctcacaaa aatattaaac 30660

ctctttctga tggagtcgta aaaaagtttt atctctttct ccttcgatgg tctcacaaaa 30720

atattaaacc tctttctgat ggagtcgtaa aaaagtttta tctctttctc tcttcgatgg 30780

tctcacaaaa atattaaacc tctttctgat ggtgtcacta aaatattttt attctctttc 30840

tctcttcgat ggtctcacaa aaatattaaa cctctttctg atggagtcgt aaaaaagttt 30900

tatctctttc tccttcgatg gtctcacaaa aatattaaac ctctttctga tggagtcgta 30960

aaaaagtttt atctctttct ctcttcgatg gtctcacaaa aatattaaac ctctttctga 31020

tggagtcgta aaaaagtttt atctctttct ccttcgatgg tctcacaaaa atattaaacc 31080

tctttctgat ggagtcgtaa aaaagtttta tctctttctc cttcgatggt ctcacaaaaa 31140

tattaaacct ctttctgatg gagtcgtaaa aaagttttat ctctttctcc ttcgatggtc 31200

tcacaaaaat attaaacctc tttctgatgg agtcgtaaaa aagttttatc tctttctctc 31260

ttcgatggtc tcacaaaaat attaaacctc tttctgatgg agtcgtaaaa aagttttatc 31320

tctttctctc ttcgatggtc tcacaaaaat attaaacctc tttctgatgg agtcgtaaaa 31380

aagttttatc tctttctcct tcgatggtct cacaaaaata ttaaacctct ttctgatggt 31440

ctctataaag cgattgattt ttcttaccct ctagagtttc ctacggtcgt tggtcacaca 31500

tttttttcta gacactaaat aaatatttaa aatataatat taatatacta aaatttatgt 31560

attattaatt tataatatta atatactaaa atttatgtat tattaattta tctaactaaa 31620

gttagtaaat catatacata attttataat taatattata tacataattt tataattaat 31680

ttaatcttac 31690

<210> 71

<211> 3948

<212> DNA

<213> 人工序列

<220>

<223> 疫苗构建体

<400> 71

atggatgcta tgaagagggg cctgtgctgc gtgctgctgc tgtgtggcgc cgtgtttgtg 60

tcccccagcc aggaaatcca cgcccggttc agaagaggca gcaagctggc tgctcagctg 120

gccgacagcg acagcaatgc ctgcgccttc ctgaagctga gatacctgca cagccggatc 180

cacgtgctgc agtttctgaa ccccttcacc ctgcacgagt acatgctgga cctgcagccc 240

gagacaaccg acctgtactg ctacgagcag gacgaggatg aggacgaggt ggaccatctg 300

caggaacagc cccagcaggc cagaagggat gagcagcacc cctgctacct gatcgagaca 360

cagtgctgca gatgcgagag cctggtggcc cagctggctg acgtgaactc taacgccgca 420

gcctttctga agaacagcat cgactgcaac gacagcatgt gcagcacctt cgacgacaac 480

gtgtccgcca ccgagctcgt gaagagaatc cctgcccctt gcccctgggc ccctgaggaa 540

aatgacgaga tcgacggcgt gaaccaccag catctgcccg ctagaagggc cgagcctcag 600

agacacacca tgctgtgcat gtgctgcaag tgcgaggccc ggatctgctt cctgctgtgc 660

ttttgtgtgc tgctgtgcgt gtgcctgctg atcagacccc tgctgctgag tgtgtccacc 720

tactgccagc ggccgctgtg tcctcaggaa aagaaacggc acgtggacct gaacaagcgg 780

ttccacatct acatctgcga ggacgcccag tgcaccgtgg tggaaggcca ggtggacagc 840

aaggtgtccg agttccggtg gtacagatac agcgtgtacg gcaccaccct gggccagcgg 900

agaatcaaga ggcccagatc cgaggtgtac tgcaagggac agctgaccga gacagaggtg 960

ctggacttcg ccttcaccga cctgaccatc gtgtaccggg acatccggct ggaatgcgcc 1020

atcatgtaca aggccagaga gatgggcttc cacgaccaca tcgactactg gaagctgatt 1080

agactggaat gtgctatctt ctacaaagcc cggatcctga agtgcctgcg gtacagattc 1140

aagaagcact gcaagctgta cgtggcctgg gactccgtgt actactgcgg cgacgatggc 1200

tggtgcaaga ccagctctac ctggcactgg acatgccacg acggcaagca caagaacgtg 1260

tgccaggaca agatcctgga acactacgag aacgactcca aggacgacga ggacgagaca 1320

gcctacgaca gcggcaccga tctgatcgac ttcatcgacg atagcaactg ccaccccaac 1380

aagctgctgc ggagactgag cagcgaccag gaccagtctc agaggccccc caacatggga 1440

gtgaaggccc acggcaagtg catctgggag aacaaggtgt tcatcgtgcc caccctgtgc 1500

cccgtgcctc tggatccaac ataccccctg ctgaagctgc tgacccccga aaccacagat 1560

ctgcactgtt atgagcagct gggcgactcc tccgacgaag aggatacagg cggcctggat 1620

ggctacgagg ccgacaagaa cgacctgaac gcccagatcg agcactggaa actgatccgg 1680

atggaatgtg caattttcta taaggccaaa gagctgggga tcagcgacga gaatgagaac 1740

gacagcgata ccggcgagga catggtggat ttcatcgaca atgaggccga gaagtacggc 1800

tgcaagggca cctgggaggt gcacttcggc tttaagaagc acggcatcac catcaccgtg 1860

cagtacgaca acgacaaggc caacaccatg gactacacca actggaaaga gatctacccc 1920

cctccccccc cacggccttg ggctcctcca attcctaagc cctctccatg ggcccctcag 1980

ttcgacggcg acatctgcaa taccatgcac tataccaatt gggtggtgta cagagacagc 2040

atcccccacg ccgcctgcca caagtgtatc gacttctaca gcagaatcag agagctgcgg 2100

cactacagcg actctgtgta cggcgatacc ctggaaaagc tgaccaacac cggcctgtac 2160

aatctgctga tccggtgcct gaggctcgtg accaagtatc ctctgctgaa actgctgtcc 2220

aactgcatcc tgtacggcgc tgccaatacc ggcaagagcc tgttcggcat gagcctgagc 2280

aaagtgcgga agctgaggta ctacaactgc tccgtgtatg gggccagcct gtgcgtggaa 2340

tgcaagaaaa ccctgcagcg gagcgaagtg tacgacgacg aaaccgacga ggaaagcacc 2400

gagagcgacc tggacggctt catcgataac agcgtgatcg tgtgccccgc ctccatcccc 2460

tccgatgaga tctctaccga ggaagccccc agaccccctc actgtccttg ggtgccagtg 2520

ttctgcaaga aggccctgac cgcctctgag gtgtacaatt ttgcctatac cgacctgcgc 2580

gtggtgtata gggacattct ggaacattat gagaatgata gcaaggacct gtgcgatcac 2640

atcaactgcc tcgtgatcta cggccctcct aacaccggca agtcctgctt cgccatgtcc 2700

ctgtggaaca ccgagcccaa gcactgcttc aagaagggcg gccagcacat cgaagtgtgg 2760

ttcgatattg tgtacaggga cggcaaccct tacgccgtgt gcgacaagtg cctgaagttc 2820

tactccaaga tcagcgagta ccgccactac tgctactccc tgtatggcac aacactggaa 2880

cagcagtaca acaagcccct gtgcgacctg ctgattcgct gcatcaacac caccagatac 2940

cctctgctgt ccctgctgaa cagctacagc accccccctc atcggattcc cgccccatgt 3000

ccatgggctc cacagaggcc tacccagacc accacccccg agaatacctc cctggtggaa 3060

ctgagagtga ccacccccaa gagcacagtc gtgatcaggc tgcacctgtg gccctacctg 3120

cactccagac tggtggtgtt caccttcccc aacccctttc accaggtggt gcccgccctg 3180

aatatctgca aggccaaggc ctgcaaagcc atcgagaaga aatgggaagt gcacgctggc 3240

ggccaagtga tcctgtgtcc tgagagcctg cggcctctgc tgctgtccat tagcgtgtac 3300

gcccaggtgc tggtgctggt gctgctgctg tgggtgtcca tcggcagcaa ctgtctggtg 3360

ctgtgcggcc ctgccaacac agggaagagt tacttcggca tgtctctgat ctgccatcag 3420

gtggtgcctc cactggccgc ctctaaggct aaagcctgtc aggccatcga actgcagctg 3480

gccctggaag ccctgaatgc cagcccctat gatcacattg attactggaa agccatccgg 3540

caggaaaatg ccatcttctt cgccgccaga tggcattgga cctgtcacga tggaaaacac 3600

aagaatgcca ttgtgaccct gacctacctg ctgcccagcg tgtgtatgtg cgcctacgct 3660

tgggtgctgg tgttcgtgta catcgtcgtg attaccagcc ccgccaccgc cgatgagtgg 3720

acactgcagc agacaagcct ggaaatgtgg ctggccgagc cccagtgtga ccatatcgat 3780

tattggaaac acatccgcct ggaatgtgct attatgtata aggcccggtg gccttacctg 3840

gaaagcagaa ccgtgttcga gttccccaat gccttcgccg gctctggacc tggcgcctct 3900

ggaaaaccca tccccaatcc actgctgggc ctggactcca cccggacc 3948

<210> 72

<211> 1316

<212> PRT

<213> 人工序列

<220>

<223> 疫苗构建体

<400> 72

Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly

1 5 10 15

Ala Val Phe Val Ser Pro Ser Gln Glu Ile His Ala Arg Phe Arg Arg

20 25 30

Gly Ser Lys Leu Ala Ala Gln Leu Ala Asp Ser Asp Ser Asn Ala Cys

35 40 45

Ala Phe Leu Lys Leu Arg Tyr Leu His Ser Arg Ile His Val Leu Gln

50 55 60

Phe Leu Asn Pro Phe Thr Leu His Glu Tyr Met Leu Asp Leu Gln Pro

65 70 75 80

Glu Thr Thr Asp Leu Tyr Cys Tyr Glu Gln Asp Glu Asp Glu Asp Glu

85 90 95

Val Asp His Leu Gln Glu Gln Pro Gln Gln Ala Arg Arg Asp Glu Gln

100 105 110

His Pro Cys Tyr Leu Ile Glu Thr Gln Cys Cys Arg Cys Glu Ser Leu

115 120 125

Val Ala Gln Leu Ala Asp Val Asn Ser Asn Ala Ala Ala Phe Leu Lys

130 135 140

Asn Ser Ile Asp Cys Asn Asp Ser Met Cys Ser Thr Phe Asp Asp Asn

145 150 155 160

Val Ser Ala Thr Glu Leu Val Lys Arg Ile Pro Ala Pro Cys Pro Trp

165 170 175

Ala Pro Glu Glu Asn Asp Glu Ile Asp Gly Val Asn His Gln His Leu

180 185 190

Pro Ala Arg Arg Ala Glu Pro Gln Arg His Thr Met Leu Cys Met Cys

195 200 205

Cys Lys Cys Glu Ala Arg Ile Cys Phe Leu Leu Cys Phe Cys Val Leu

210 215 220

Leu Cys Val Cys Leu Leu Ile Arg Pro Leu Leu Leu Ser Val Ser Thr

225 230 235 240

Tyr Cys Gln Arg Pro Leu Cys Pro Gln Glu Lys Lys Arg His Val Asp

245 250 255

Leu Asn Lys Arg Phe His Ile Tyr Ile Cys Glu Asp Ala Gln Cys Thr

260 265 270

Val Val Glu Gly Gln Val Asp Ser Lys Val Ser Glu Phe Arg Trp Tyr

275 280 285

Arg Tyr Ser Val Tyr Gly Thr Thr Leu Gly Gln Arg Arg Ile Lys Arg

290 295 300

Pro Arg Ser Glu Val Tyr Cys Lys Gly Gln Leu Thr Glu Thr Glu Val

305 310 315 320

Leu Asp Phe Ala Phe Thr Asp Leu Thr Ile Val Tyr Arg Asp Ile Arg

325 330 335

Leu Glu Cys Ala Ile Met Tyr Lys Ala Arg Glu Met Gly Phe His Asp

340 345 350

His Ile Asp Tyr Trp Lys Leu Ile Arg Leu Glu Cys Ala Ile Phe Tyr

355 360 365

Lys Ala Arg Ile Leu Lys Cys Leu Arg Tyr Arg Phe Lys Lys His Cys

370 375 380

Lys Leu Tyr Val Ala Trp Asp Ser Val Tyr Tyr Cys Gly Asp Asp Gly

385 390 395 400

Trp Cys Lys Thr Ser Ser Thr Trp His Trp Thr Cys His Asp Gly Lys

405 410 415

His Lys Asn Val Cys Gln Asp Lys Ile Leu Glu His Tyr Glu Asn Asp

420 425 430

Ser Lys Asp Asp Glu Asp Glu Thr Ala Tyr Asp Ser Gly Thr Asp Leu

435 440 445

Ile Asp Phe Ile Asp Asp Ser Asn Cys His Pro Asn Lys Leu Leu Arg

450 455 460

Arg Leu Ser Ser Asp Gln Asp Gln Ser Gln Arg Pro Pro Asn Met Gly

465 470 475 480

Val Lys Ala His Gly Lys Cys Ile Trp Glu Asn Lys Val Phe Ile Val

485 490 495

Pro Thr Leu Cys Pro Val Pro Leu Asp Pro Thr Tyr Pro Leu Leu Lys

500 505 510

Leu Leu Thr Pro Glu Thr Thr Asp Leu His Cys Tyr Glu Gln Leu Gly

515 520 525

Asp Ser Ser Asp Glu Glu Asp Thr Gly Gly Leu Asp Gly Tyr Glu Ala

530 535 540

Asp Lys Asn Asp Leu Asn Ala Gln Ile Glu His Trp Lys Leu Ile Arg

545 550 555 560

Met Glu Cys Ala Ile Phe Tyr Lys Ala Lys Glu Leu Gly Ile Ser Asp

565 570 575

Glu Asn Glu Asn Asp Ser Asp Thr Gly Glu Asp Met Val Asp Phe Ile

580 585 590

Asp Asn Glu Ala Glu Lys Tyr Gly Cys Lys Gly Thr Trp Glu Val His

595 600 605

Phe Gly Phe Lys Lys His Gly Ile Thr Ile Thr Val Gln Tyr Asp Asn

610 615 620

Asp Lys Ala Asn Thr Met Asp Tyr Thr Asn Trp Lys Glu Ile Tyr Pro

625 630 635 640

Pro Pro Pro Pro Arg Pro Trp Ala Pro Pro Ile Pro Lys Pro Ser Pro

645 650 655

Trp Ala Pro Gln Phe Asp Gly Asp Ile Cys Asn Thr Met His Tyr Thr

660 665 670

Asn Trp Val Val Tyr Arg Asp Ser Ile Pro His Ala Ala Cys His Lys

675 680 685

Cys Ile Asp Phe Tyr Ser Arg Ile Arg Glu Leu Arg His Tyr Ser Asp

690 695 700

Ser Val Tyr Gly Asp Thr Leu Glu Lys Leu Thr Asn Thr Gly Leu Tyr

705 710 715 720

Asn Leu Leu Ile Arg Cys Leu Arg Leu Val Thr Lys Tyr Pro Leu Leu

725 730 735

Lys Leu Leu Ser Asn Cys Ile Leu Tyr Gly Ala Ala Asn Thr Gly Lys

740 745 750

Ser Leu Phe Gly Met Ser Leu Ser Lys Val Arg Lys Leu Arg Tyr Tyr

755 760 765

Asn Cys Ser Val Tyr Gly Ala Ser Leu Cys Val Glu Cys Lys Lys Thr

770 775 780

Leu Gln Arg Ser Glu Val Tyr Asp Asp Glu Thr Asp Glu Glu Ser Thr

785 790 795 800

Glu Ser Asp Leu Asp Gly Phe Ile Asp Asn Ser Val Ile Val Cys Pro

805 810 815

Ala Ser Ile Pro Ser Asp Glu Ile Ser Thr Glu Glu Ala Pro Arg Pro

820 825 830

Pro His Cys Pro Trp Val Pro Val Phe Cys Lys Lys Ala Leu Thr Ala

835 840 845

Ser Glu Val Tyr Asn Phe Ala Tyr Thr Asp Leu Arg Val Val Tyr Arg

850 855 860

Asp Ile Leu Glu His Tyr Glu Asn Asp Ser Lys Asp Leu Cys Asp His

865 870 875 880

Ile Asn Cys Leu Val Ile Tyr Gly Pro Pro Asn Thr Gly Lys Ser Cys

885 890 895

Phe Ala Met Ser Leu Trp Asn Thr Glu Pro Lys His Cys Phe Lys Lys

900 905 910

Gly Gly Gln His Ile Glu Val Trp Phe Asp Ile Val Tyr Arg Asp Gly

915 920 925

Asn Pro Tyr Ala Val Cys Asp Lys Cys Leu Lys Phe Tyr Ser Lys Ile

930 935 940

Ser Glu Tyr Arg His Tyr Cys Tyr Ser Leu Tyr Gly Thr Thr Leu Glu

945 950 955 960

Gln Gln Tyr Asn Lys Pro Leu Cys Asp Leu Leu Ile Arg Cys Ile Asn

965 970 975

Thr Thr Arg Tyr Pro Leu Leu Ser Leu Leu Asn Ser Tyr Ser Thr Pro

980 985 990

Pro His Arg Ile Pro Ala Pro Cys Pro Trp Ala Pro Gln Arg Pro Thr

995 1000 1005

Gln Thr Thr Thr Pro Glu Asn Thr Ser Leu Val Glu Leu Arg Val

1010 1015 1020

Thr Thr Pro Lys Ser Thr Val Val Ile Arg Leu His Leu Trp Pro

1025 1030 1035

Tyr Leu His Ser Arg Leu Val Val Phe Thr Phe Pro Asn Pro Phe

1040 1045 1050

His Gln Val Val Pro Ala Leu Asn Ile Cys Lys Ala Lys Ala Cys

1055 1060 1065

Lys Ala Ile Glu Lys Lys Trp Glu Val His Ala Gly Gly Gln Val

1070 1075 1080

Ile Leu Cys Pro Glu Ser Leu Arg Pro Leu Leu Leu Ser Ile Ser

1085 1090 1095

Val Tyr Ala Gln Val Leu Val Leu Val Leu Leu Leu Trp Val Ser

1100 1105 1110

Ile Gly Ser Asn Cys Leu Val Leu Cys Gly Pro Ala Asn Thr Gly

1115 1120 1125

Lys Ser Tyr Phe Gly Met Ser Leu Ile Cys His Gln Val Val Pro

1130 1135 1140

Pro Leu Ala Ala Ser Lys Ala Lys Ala Cys Gln Ala Ile Glu Leu

1145 1150 1155

Gln Leu Ala Leu Glu Ala Leu Asn Ala Ser Pro Tyr Asp His Ile

1160 1165 1170

Asp Tyr Trp Lys Ala Ile Arg Gln Glu Asn Ala Ile Phe Phe Ala

1175 1180 1185

Ala Arg Trp His Trp Thr Cys His Asp Gly Lys His Lys Asn Ala

1190 1195 1200

Ile Val Thr Leu Thr Tyr Leu Leu Pro Ser Val Cys Met Cys Ala

1205 1210 1215

Tyr Ala Trp Val Leu Val Phe Val Tyr Ile Val Val Ile Thr Ser

1220 1225 1230

Pro Ala Thr Ala Asp Glu Trp Thr Leu Gln Gln Thr Ser Leu Glu

1235 1240 1245

Met Trp Leu Ala Glu Pro Gln Cys Asp His Ile Asp Tyr Trp Lys

1250 1255 1260

His Ile Arg Leu Glu Cys Ala Ile Met Tyr Lys Ala Arg Trp Pro

1265 1270 1275

Tyr Leu Glu Ser Arg Thr Val Phe Glu Phe Pro Asn Ala Phe Ala

1280 1285 1290

Gly Ser Gly Pro Gly Ala Ser Gly Lys Pro Ile Pro Asn Pro Leu

1295 1300 1305

Leu Gly Leu Asp Ser Thr Arg Thr

1310 1315

<210> 73

<211> 3321

<212> DNA

<213> 人工序列

<220>

<223> 疫苗构建体

<400> 73

atggatgcta tgaagcgagg actgtgctgc gtgctgctgc tgtgtggcgc tgtgtttgtg 60

tcccctagcc aagagatcca cgccagattc agacggggca gcaaactggc cgacgaggat 120

gagacagcct acgactctgg caccgacctg atcgacttca tcgacgacag cgacgagaac 180

gagaatgaca gcgacaccgg cgaggacatg gtggatttca tcgacaatgc ccagctggcc 240

gactccgact ctaatgcctg tgcctttctg aaggctcagc tggctgacgt gaacagcaat 300

gccgccgctt tcctgaagaa ctgcatcctg ctgtacggcg ctgccaacac aggcaagagc 360

ctgtttggca tgagcctgaa ctgcctggtg ctgtgcggac ctgccaatac cggcaaaagc 420

tacttcggca tgtccctgtg gccttacctg cacagcagac tggtggtgtt tacattcccc 480

aatcctttct ggccctacct ggaaagccgg atcaccgtgt tcgagttccc caacgccttc 540

aacgtgtgcc aggacaagat cctggaacac tatgagaacg acagcaagga catccttgag 600

cactacgaaa acgactccaa ggacctgtgc gaccacatct gcgatcacat cgactactgg 660

aagcacatcc ggctggaatg cgccatcatg tacaaggccc ggatcagact ggaatgtgct 720

attatgtata aggctcgcga gatgggcttc caccagttcg acggcgacat ctgcaacacc 780

atgcactaca ccaactggat ctatatctgc gaggacgccc agtgcaccgt ggtggaagga 840

caggtggaca agaaatggga agtgcacgct ggcggccaag tgattctgtg tcctgagagc 900

ggccagcgga gaatcaagag gcccagatcc gagaactgtc accccaacaa gctgctgatc 960

ctgaagtgcc tgcggtacag attcaagaag cactgcaagc tgagcagcac ctggcactgg 1020

acatgccacg atggcaagca caagtggcat tggacctgtc acgacgggaa acacaagaac 1080

gccatcgtga ccctgaccta ctacgaggcc gacaagaacg acctgaacgc ccagattgag 1140

cactggaaac tgatccggat ggaatgtgca atcttctata aggccaaaga gctggggatc 1200

agcatctgcc accaggtggt gcctccactg gctgcctcta aagccaaagc ctgtcaggcc 1260

atcgaactgc agctggccct ggaagccctg aacgctagcc cttacgatga gtggaccctg 1320

cagcagacca gcctggaaat gtggctggcc gagcctcagt ttaagaagca cggcatcacc 1380

atcaccgtgc agtacgacaa cgacaaggcc aataccatgg attacacgaa ttggaaagaa 1440

atctacgtga tcgtgtgccc cgccagcatt ccctccgatg agatctctac cgaggaagcc 1500

gaccacattg attattggaa ggccatccgg caagagaatg ccatcttctt cgccgccaga 1560

catcaggtgg tccccgctct gaatatctgc aaggccaagg cctgcaaagc catcgagtgg 1620

aacaccgagc ctaagcactg cttcaagaaa ggcggccagc acatcgaagt ttggttcgac 1680

tacgtggcct gggacagcgt gtactactgc ggagatgatg gctggtgcaa gaccgaggcc 1740

gagaagtacg gctgtaaagg cacctgggaa gtccacttcg gcaacagcat cgactgcaac 1800

gatagcatgt gcagcacctt cgacgacaac gtgtccgcca cagagctggt caaggaccat 1860

atagactatt ggaagctgat caggcttgag tgcgccattt tctacaaggc cagacggcgg 1920

ctgtccagcg accaggatca atctcagctc gtgaccaagt atcccctgct gaagctgctg 1980

tctacccaga ccaccacacc tgagaacaca agcctggtgg aactgagagt gaccacacct 2040

aagagcaccg tcgtgattcg gctgcacctg accacaagat accctctgct gagcctgctg 2100

aacagctaca gcacccctcc acacaggatc cccgctccat gtccttgggc tcctcagagg 2160

cctcctattc ctaagccttc tccatgggct cctagaatcc ccgcaccttg tccatgggca 2220

ccaccaagac ctccacattg cccttgggtg ccctgtttcc tgctgtgctt ttgcgtgctc 2280

ctgtgcgtgt gcctgctgat cagacctctg ctgctgagcg tgtccaccta ccttagacca 2340

ctgctcctgt ccatctccgt gtacgcacag gtgctggtgc tggtcctgct tctgtgggtg 2400

tccatcggaa gcctgctgcc tagcgtgtgc atgtgtgcct atgcttgggt gctcgtgttc 2460

gtgtacatcg tggtcatcac aagccccgcc acagccatcg tgtacagaga tggcaatccc 2520

tacgccgtgt gcgacaagtg cctgaagttc tacagcaaga tcagcgagta ccggcactac 2580

tgctacagcc tgtacggcac cacactggaa cagcagtaca acaagcccct gtgcgatctg 2640

ctgattcggt gcatcaacgt ggtgtaccgg gacagcattc ctcacgccgc ctgccacaag 2700

tgcatcgact tctactccag aatcagagag ctgcggcact acagcgactc tgtgtacggc 2760

gacaccctgg aaaagctgac caacaccggc ctgtacaacc tgctgattag atgcctgcgg 2820

gtgtactgca agggacagct gacagagaca gaggtgctgg acttcgcctt caccgatctg 2880

acaatcgtgt atcgggatag caaggtgtcc gagttccggt ggtacagata tagcgtgtac 2940

ggaacaaccc tgtgcgtcga gtgcaagaaa accctgcaga gaagcgaggt gtacgactgc 3000

cagaggccac tgtgccctca agagaagaaa cggcacgtgg acctgaacaa gcggtttcac 3060

accctgcacg agtacatgct ggacctgcag cctgagacaa ccgacctgta ctgctacgag 3120

cagcccgaaa ccacagatct gcactgttat gagcagctgg gcgacagcag cgacgaagag 3180

gatacaggcg gactggacgg cgaggaaaac gacgaaattg acggcgtgaa ccaccagcat 3240

ctccccgcca gaagggctga acctcagaga cacaccatgc tgtgtatgtg ctgcaagtgc 3300

gaggccagaa tcgcctgatg a 3321

<210> 74

<211> 1105

<212> PRT

<213> 人工序列

<220>

<223> 疫苗构建体

<400> 74

Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly

1 5 10 15

Ala Val Phe Val Ser Pro Ser Gln Glu Ile His Ala Arg Phe Arg Arg

20 25 30

Gly Ser Lys Leu Ala Asp Glu Asp Glu Thr Ala Tyr Asp Ser Gly Thr

35 40 45

Asp Leu Ile Asp Phe Ile Asp Asp Ser Asp Glu Asn Glu Asn Asp Ser

50 55 60

Asp Thr Gly Glu Asp Met Val Asp Phe Ile Asp Asn Ala Gln Leu Ala

65 70 75 80

Asp Ser Asp Ser Asn Ala Cys Ala Phe Leu Lys Ala Gln Leu Ala Asp

85 90 95

Val Asn Ser Asn Ala Ala Ala Phe Leu Lys Asn Cys Ile Leu Leu Tyr

100 105 110

Gly Ala Ala Asn Thr Gly Lys Ser Leu Phe Gly Met Ser Leu Asn Cys

115 120 125

Leu Val Leu Cys Gly Pro Ala Asn Thr Gly Lys Ser Tyr Phe Gly Met

130 135 140

Ser Leu Trp Pro Tyr Leu His Ser Arg Leu Val Val Phe Thr Phe Pro

145 150 155 160

Asn Pro Phe Trp Pro Tyr Leu Glu Ser Arg Ile Thr Val Phe Glu Phe

165 170 175

Pro Asn Ala Phe Asn Val Cys Gln Asp Lys Ile Leu Glu His Tyr Glu

180 185 190

Asn Asp Ser Lys Asp Ile Leu Glu His Tyr Glu Asn Asp Ser Lys Asp

195 200 205

Leu Cys Asp His Ile Cys Asp His Ile Asp Tyr Trp Lys His Ile Arg

210 215 220

Leu Glu Cys Ala Ile Met Tyr Lys Ala Arg Ile Arg Leu Glu Cys Ala

225 230 235 240

Ile Met Tyr Lys Ala Arg Glu Met Gly Phe His Gln Phe Asp Gly Asp

245 250 255

Ile Cys Asn Thr Met His Tyr Thr Asn Trp Ile Tyr Ile Cys Glu Asp

260 265 270

Ala Gln Cys Thr Val Val Glu Gly Gln Val Asp Lys Lys Trp Glu Val

275 280 285

His Ala Gly Gly Gln Val Ile Leu Cys Pro Glu Ser Gly Gln Arg Arg

290 295 300

Ile Lys Arg Pro Arg Ser Glu Asn Cys His Pro Asn Lys Leu Leu Ile

305 310 315 320

Leu Lys Cys Leu Arg Tyr Arg Phe Lys Lys His Cys Lys Leu Ser Ser

325 330 335

Thr Trp His Trp Thr Cys His Asp Gly Lys His Lys Trp His Trp Thr

340 345 350

Cys His Asp Gly Lys His Lys Asn Ala Ile Val Thr Leu Thr Tyr Tyr

355 360 365

Glu Ala Asp Lys Asn Asp Leu Asn Ala Gln Ile Glu His Trp Lys Leu

370 375 380

Ile Arg Met Glu Cys Ala Ile Phe Tyr Lys Ala Lys Glu Leu Gly Ile

385 390 395 400

Ser Ile Cys His Gln Val Val Pro Pro Leu Ala Ala Ser Lys Ala Lys

405 410 415

Ala Cys Gln Ala Ile Glu Leu Gln Leu Ala Leu Glu Ala Leu Asn Ala

420 425 430

Ser Pro Tyr Asp Glu Trp Thr Leu Gln Gln Thr Ser Leu Glu Met Trp

435 440 445

Leu Ala Glu Pro Gln Phe Lys Lys His Gly Ile Thr Ile Thr Val Gln

450 455 460

Tyr Asp Asn Asp Lys Ala Asn Thr Met Asp Tyr Thr Asn Trp Lys Glu

465 470 475 480

Ile Tyr Val Ile Val Cys Pro Ala Ser Ile Pro Ser Asp Glu Ile Ser

485 490 495

Thr Glu Glu Ala Asp His Ile Asp Tyr Trp Lys Ala Ile Arg Gln Glu

500 505 510

Asn Ala Ile Phe Phe Ala Ala Arg His Gln Val Val Pro Ala Leu Asn

515 520 525

Ile Cys Lys Ala Lys Ala Cys Lys Ala Ile Glu Trp Asn Thr Glu Pro

530 535 540

Lys His Cys Phe Lys Lys Gly Gly Gln His Ile Glu Val Trp Phe Asp

545 550 555 560

Tyr Val Ala Trp Asp Ser Val Tyr Tyr Cys Gly Asp Asp Gly Trp Cys

565 570 575

Lys Thr Glu Ala Glu Lys Tyr Gly Cys Lys Gly Thr Trp Glu Val His

580 585 590

Phe Gly Asn Ser Ile Asp Cys Asn Asp Ser Met Cys Ser Thr Phe Asp

595 600 605

Asp Asn Val Ser Ala Thr Glu Leu Val Lys Asp His Ile Asp Tyr Trp

610 615 620

Lys Leu Ile Arg Leu Glu Cys Ala Ile Phe Tyr Lys Ala Arg Arg Arg

625 630 635 640

Leu Ser Ser Asp Gln Asp Gln Ser Gln Leu Val Thr Lys Tyr Pro Leu

645 650 655

Leu Lys Leu Leu Ser Thr Gln Thr Thr Thr Pro Glu Asn Thr Ser Leu

660 665 670

Val Glu Leu Arg Val Thr Thr Pro Lys Ser Thr Val Val Ile Arg Leu

675 680 685

His Leu Thr Thr Arg Tyr Pro Leu Leu Ser Leu Leu Asn Ser Tyr Ser

690 695 700

Thr Pro Pro His Arg Ile Pro Ala Pro Cys Pro Trp Ala Pro Gln Arg

705 710 715 720

Pro Pro Ile Pro Lys Pro Ser Pro Trp Ala Pro Arg Ile Pro Ala Pro

725 730 735

Cys Pro Trp Ala Pro Pro Arg Pro Pro His Cys Pro Trp Val Pro Cys

740 745 750

Phe Leu Leu Cys Phe Cys Val Leu Leu Cys Val Cys Leu Leu Ile Arg

755 760 765

Pro Leu Leu Leu Ser Val Ser Thr Tyr Leu Arg Pro Leu Leu Leu Ser

770 775 780

Ile Ser Val Tyr Ala Gln Val Leu Val Leu Val Leu Leu Leu Trp Val

785 790 795 800

Ser Ile Gly Ser Leu Leu Pro Ser Val Cys Met Cys Ala Tyr Ala Trp

805 810 815

Val Leu Val Phe Val Tyr Ile Val Val Ile Thr Ser Pro Ala Thr Ala

820 825 830

Ile Val Tyr Arg Asp Gly Asn Pro Tyr Ala Val Cys Asp Lys Cys Leu

835 840 845

Lys Phe Tyr Ser Lys Ile Ser Glu Tyr Arg His Tyr Cys Tyr Ser Leu

850 855 860

Tyr Gly Thr Thr Leu Glu Gln Gln Tyr Asn Lys Pro Leu Cys Asp Leu

865 870 875 880

Leu Ile Arg Cys Ile Asn Val Val Tyr Arg Asp Ser Ile Pro His Ala

885 890 895

Ala Cys His Lys Cys Ile Asp Phe Tyr Ser Arg Ile Arg Glu Leu Arg

900 905 910

His Tyr Ser Asp Ser Val Tyr Gly Asp Thr Leu Glu Lys Leu Thr Asn

915 920 925

Thr Gly Leu Tyr Asn Leu Leu Ile Arg Cys Leu Arg Val Tyr Cys Lys

930 935 940

Gly Gln Leu Thr Glu Thr Glu Val Leu Asp Phe Ala Phe Thr Asp Leu

945 950 955 960

Thr Ile Val Tyr Arg Asp Ser Lys Val Ser Glu Phe Arg Trp Tyr Arg

965 970 975

Tyr Ser Val Tyr Gly Thr Thr Leu Cys Val Glu Cys Lys Lys Thr Leu

980 985 990

Gln Arg Ser Glu Val Tyr Asp Cys Gln Arg Pro Leu Cys Pro Gln Glu

995 1000 1005

Lys Lys Arg His Val Asp Leu Asn Lys Arg Phe His Thr Leu His

1010 1015 1020

Glu Tyr Met Leu Asp Leu Gln Pro Glu Thr Thr Asp Leu Tyr Cys

1025 1030 1035

Tyr Glu Gln Pro Glu Thr Thr Asp Leu His Cys Tyr Glu Gln Leu

1040 1045 1050

Gly Asp Ser Ser Asp Glu Glu Asp Thr Gly Gly Leu Asp Gly Glu

1055 1060 1065

Glu Asn Asp Glu Ile Asp Gly Val Asn His Gln His Leu Pro Ala

1070 1075 1080

Arg Arg Ala Glu Pro Gln Arg His Thr Met Leu Cys Met Cys Cys

1085 1090 1095

Lys Cys Glu Ala Arg Ile Ala

1100 1105

<210> 75

<211> 4470

<212> DNA

<213> 人工序列

<220>

<223> 疫苗构建体

<400> 75

atggatgcta tgaagagggg cctgtgctgc gtgctgctgc tgtgtggcgc cgtgtttgtg 60

tcccccagcc aggaaatcca cgcccggttc agaagaggca gcaagctggc cgacgaggac 120

gagacagcct acgacagcgg caccgacctg atcgacttca tcgacgatag cgccgctgcc 180

gacgagaatg agaacgacag cgataccggc gaggacatgg tggatttcat cgacaacgct 240

gccgccgacg aaaccgacga agagagcacc gagagcgacc tggacggctt tatcgacaac 300

agcgcagccg cccagctggc tgacagcgac tctaatgcct gcgccttcct gaaggccgct 360

gctcagctgg cagacgtgaa cagcaatgcc gccgcttttc tgaaggctgc cgccaactgc 420

atcctgctgt acggcgctgc caacaccggc aagagcctgt tcggcatgtc tctggccgca 480

gccaactgcc tggtgctgtg cggacctgcc aatactggca aaagctactt cggcatgagc 540

ctggcagccg ccaattgtct cgtgatctac ggccctccta ataccggcaa gtcctgcttt 600

gccatgagtc tggccgctgc ctggccctac ctgcactcta gactggtggt gttcaccttc 660

cccaacccct tcgctgccgc ttggccttac ctggaaagcc ggatcaccgt gttcgagttc 720

cccaatgcct tcgccgcagc cctgagatac ctgcacagca gaatccacgt gctgcagttt 780

ctgaacccct ttgccgccgc aaacgtgtgc caggacaaga tcctggaaca ctacgagaac 840

gactccaagg atgccgctgc cattctggaa cattatgaga atgatagcaa ggacctgtgc 900

gaccacattg ctgccgcctg cgatcacatc gactactgga agcacatccg gctggaatgc 960

gccatcatgt acaaggccag agccgccgct atcagactgg aatgtgctat tatgtataag 1020

gctcgcgaga tgggcttcca cgctgctgcc cagttcgacg gcgacatctg caacaccatg 1080

cactacacca actgggctgc cgctatctac atctgcgagg acgcccagtg caccgtggtg 1140

gaaggacagg tggacgccgc tgctaagaaa tgggaggtgc acgctggcgg ccaagtgatc 1200

ctgtgtccag agtctgctgc cgcaggccag cggagaatca agaggcctag aagcgaggca 1260

gccgctaact gccaccccaa caaactgctg gctgctgcca tcctgaagtg cctgcggtac 1320

agattcaaga agcactgcaa actggctgca gctagcagca cctggcactg gacctgtcac 1380

gacggcaagc acaaagccgc cgcatggcat tggacatgcc acgatggaaa acacaagaac 1440

gccatcgtga ccctgaccta tgcagccgcc tacgaggccg acaagaacga cctgaacgcc 1500

cagatcgagc actggaagct gatcaggatg gaatgtgcaa tcttctataa ggccaaagag 1560

ctgggcatca gcgctgccgc aatctgccac caggtggtgc ctccactggc cgcctctaaa 1620

gccaaagcct gccaggccat cgaactgcag ctggccctgg aagccctgaa tgccagccct 1680

tatgccgcag ccgatgagtg gaccctgcag cagaccagcc tggaaatgtg gctggccgaa 1740

cctcaggccg cagcttttaa gaagcacggc atcaccatca ccgtgcagta cgacaacgac 1800

aaggccaata ccatggatta caccaattgg aaagagatct acgccgcagc tgtgatcgtg 1860

tgccccgcca gcatccctag cgacgagatc agcacagagg aagcagccgc cgaccacatc 1920

gattattgga aagccatcag acaggaaaac gccatcttct tcgccgctag agccgctgcc 1980

caccaggtgg tgccagccct gaatatctgc aaggccaagg cctgtaaagc catcgaagcc 2040

gctgcttgga acaccgagcc caagcactgc ttcaagaagg gcggccagca catcgaagtg 2100

tggttcgacg ctgcagccta cgtggcctgg gacagcgtgt actactgtgg cgacgacggc 2160

tggtgcaaga ccgccgctgc agaggccgag aagtatggct gcaagggcac ctgggaagtg 2220

catttcggcg cagctgccaa ctccatcgac tgcaacgaca gcatgtgcag caccttcgac 2280

gacaacgtgt ccgccaccga gctcgtgaaa gctgccgctg accatattga ttactggaaa 2340

ctgattcgcc tggaatgcgc tattttctac aaagccaggg ccgcagcacg gcggctgtcc 2400

tcagatcagg atcagagcca ggctgctgca ctcgtgacca agtaccccct gctgaagctg 2460

ctgagcgccg cagcaagacc ccccaacatg ggagtgaagg cccacggcaa gtgcatctgg 2520

gagaacaagg tgttcatcgt gcccaccctg tgccccgtgc ctctggatcc aacatatcct 2580

ctgctgaaac tgctgaccgc tgccgccacc cagaccacca cacctgagaa tacctccctg 2640

gtggaactga gagtgaccac ccccaagagc acagtcgtga tcaggctgca cctggctgcc 2700

gcaaccacca gataccctct gctgtccctg ctgaacagct acagcacccc ccctcatcgg 2760

atccctgccc cttgtccttg ggctcctcag aggcctgccg ctgcacctat ccctaagcct 2820

tctccatggg cccctgccgc agctagaatc ccagctccat gtccatgggc accagctgct 2880

gctcccagac ctcctcattg cccttgggtg ccagcagccg ctcctccacc tcctcctaga 2940

ccttgggccc cagccgccgc ttgtttcctg ctgtgcttct gtgtgctgct gtgcgtgtgc 3000

ctgctgatca gacccctgct gctgagtgtg tccacctacg cagctgctct gcggccactg 3060

ctgctgtcca tctctgtgta cgcacaggtg ctggtgctgg tgctgctgct gtgggtgtcc 3120

atcggatctg ccgcagcact gctgccctcc gtgtgcatgt gtgcctatgc ctgggtgctg 3180

gtgttcgtgt acatcgtcgt gattaccagc cccgccaccg cagccgcaat cgtgtacagg 3240

gacggcaacc cttacgccgt gtgcgacaag tgcctgaagt tctacagcaa gatcagcgag 3300

taccgccact actgctacag cctgtacggc accaccctgg aacagcagta caacaagccc 3360

ctgtgcgatc tgctgatccg gtgcatcaac gcagccgctg tggtgtacag agacagcatc 3420

ccacacgccg cctgccacaa gtgtatcgac ttctactccc ggatcagaga gctgagacac 3480

tactccgact ccgtgtacgg cgataccctg gaaaagctga ccaataccgg cctgtacaac 3540

ctgctgatta gatgcctgcg ggcagccgca gtgttctgca agaaagccct gaccgccagc 3600

gaggtgtaca acttcgccta caccgatctg cgggtggtgt accgggatgc tgctgcctcc 3660

aaagtgcgga agctgcggta ctacaactgc tctgtgtatg gcgcctccct ggcagctgcc 3720

gtgtattgca agggacagct gaccgagaca gaggtgctgg atttcgcctt cacagacctg 3780

accatcgtgt atagagatgc agctgctagc aaggtgtccg agttccggtg gtacagatat 3840

agcgtgtacg gaacaacact ggcagcagct tgcgtggaat gcaagaaaac actgcagcgg 3900

agcgaagtgt acgatgctgc agcttgccag aggccgctgt gtcctcagga aaagaaaaga 3960

cacgtggacc tgaacaagcg gttccacgca gcagctaccc tgcacgagta catgctggac 4020

ctgcagcccg agacaaccga cctgtactgc tacgagcagg cagctgcacc cgaaaccaca 4080

gatctgcact gttatgagca gctgggagac agctccgatg aagaggacac cggcggactg 4140

gatgctgccg ctggggatga ggacgaggat gaggtggacc atctgcagga acagccccag 4200

caggccagaa gggatgagca gcacccctgc tatctgatcg agacacagtg ctgcagatgc 4260

gagagcctgg tggccgctgc tgaggaaaac gacgagatcg acggcgtgaa ccaccagcat 4320

ctgcccgcta gaagggccga gcctcagaga cacaccatgc tgtgtatgtg ttgcaagtgc 4380

gaggcccgga tcgccggatc tggacctggc gctagcggaa agcccatccc caatccactg 4440

ctgggcctgg actccacccg gacctgataa 4470

<210> 76

<211> 1488

<212> PRT

<213> 人工序列

<220>

<223> 疫苗构建体

<400> 76

Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly

1 5 10 15

Ala Val Phe Val Ser Pro Ser Gln Glu Ile His Ala Arg Phe Arg Arg

20 25 30

Gly Ser Lys Leu Ala Asp Glu Asp Glu Thr Ala Tyr Asp Ser Gly Thr

35 40 45

Asp Leu Ile Asp Phe Ile Asp Asp Ser Ala Ala Ala Asp Glu Asn Glu

50 55 60

Asn Asp Ser Asp Thr Gly Glu Asp Met Val Asp Phe Ile Asp Asn Ala

65 70 75 80

Ala Ala Asp Glu Thr Asp Glu Glu Ser Thr Glu Ser Asp Leu Asp Gly

85 90 95

Phe Ile Asp Asn Ser Ala Ala Ala Gln Leu Ala Asp Ser Asp Ser Asn

100 105 110

Ala Cys Ala Phe Leu Lys Ala Ala Ala Gln Leu Ala Asp Val Asn Ser

115 120 125

Asn Ala Ala Ala Phe Leu Lys Ala Ala Ala Asn Cys Ile Leu Leu Tyr

130 135 140

Gly Ala Ala Asn Thr Gly Lys Ser Leu Phe Gly Met Ser Leu Ala Ala

145 150 155 160

Ala Asn Cys Leu Val Leu Cys Gly Pro Ala Asn Thr Gly Lys Ser Tyr

165 170 175

Phe Gly Met Ser Leu Ala Ala Ala Asn Cys Leu Val Ile Tyr Gly Pro

180 185 190

Pro Asn Thr Gly Lys Ser Cys Phe Ala Met Ser Leu Ala Ala Ala Trp

195 200 205

Pro Tyr Leu His Ser Arg Leu Val Val Phe Thr Phe Pro Asn Pro Phe

210 215 220

Ala Ala Ala Trp Pro Tyr Leu Glu Ser Arg Ile Thr Val Phe Glu Phe

225 230 235 240

Pro Asn Ala Phe Ala Ala Ala Leu Arg Tyr Leu His Ser Arg Ile His

245 250 255

Val Leu Gln Phe Leu Asn Pro Phe Ala Ala Ala Asn Val Cys Gln Asp

260 265 270

Lys Ile Leu Glu His Tyr Glu Asn Asp Ser Lys Asp Ala Ala Ala Ile

275 280 285

Leu Glu His Tyr Glu Asn Asp Ser Lys Asp Leu Cys Asp His Ile Ala

290 295 300

Ala Ala Cys Asp His Ile Asp Tyr Trp Lys His Ile Arg Leu Glu Cys

305 310 315 320

Ala Ile Met Tyr Lys Ala Arg Ala Ala Ala Ile Arg Leu Glu Cys Ala

325 330 335

Ile Met Tyr Lys Ala Arg Glu Met Gly Phe His Ala Ala Ala Gln Phe

340 345 350

Asp Gly Asp Ile Cys Asn Thr Met His Tyr Thr Asn Trp Ala Ala Ala

355 360 365

Ile Tyr Ile Cys Glu Asp Ala Gln Cys Thr Val Val Glu Gly Gln Val

370 375 380

Asp Ala Ala Ala Lys Lys Trp Glu Val His Ala Gly Gly Gln Val Ile

385 390 395 400

Leu Cys Pro Glu Ser Ala Ala Ala Gly Gln Arg Arg Ile Lys Arg Pro

405 410 415

Arg Ser Glu Ala Ala Ala Asn Cys His Pro Asn Lys Leu Leu Ala Ala

420 425 430

Ala Ile Leu Lys Cys Leu Arg Tyr Arg Phe Lys Lys His Cys Lys Leu

435 440 445

Ala Ala Ala Ser Ser Thr Trp His Trp Thr Cys His Asp Gly Lys His

450 455 460

Lys Ala Ala Ala Trp His Trp Thr Cys His Asp Gly Lys His Lys Asn

465 470 475 480

Ala Ile Val Thr Leu Thr Tyr Ala Ala Ala Tyr Glu Ala Asp Lys Asn

485 490 495

Asp Leu Asn Ala Gln Ile Glu His Trp Lys Leu Ile Arg Met Glu Cys

500 505 510

Ala Ile Phe Tyr Lys Ala Lys Glu Leu Gly Ile Ser Ala Ala Ala Ile

515 520 525

Cys His Gln Val Val Pro Pro Leu Ala Ala Ser Lys Ala Lys Ala Cys

530 535 540

Gln Ala Ile Glu Leu Gln Leu Ala Leu Glu Ala Leu Asn Ala Ser Pro

545 550 555 560

Tyr Ala Ala Ala Asp Glu Trp Thr Leu Gln Gln Thr Ser Leu Glu Met

565 570 575

Trp Leu Ala Glu Pro Gln Ala Ala Ala Phe Lys Lys His Gly Ile Thr

580 585 590

Ile Thr Val Gln Tyr Asp Asn Asp Lys Ala Asn Thr Met Asp Tyr Thr

595 600 605

Asn Trp Lys Glu Ile Tyr Ala Ala Ala Val Ile Val Cys Pro Ala Ser

610 615 620

Ile Pro Ser Asp Glu Ile Ser Thr Glu Glu Ala Ala Ala Asp His Ile

625 630 635 640

Asp Tyr Trp Lys Ala Ile Arg Gln Glu Asn Ala Ile Phe Phe Ala Ala

645 650 655

Arg Ala Ala Ala His Gln Val Val Pro Ala Leu Asn Ile Cys Lys Ala

660 665 670

Lys Ala Cys Lys Ala Ile Glu Ala Ala Ala Trp Asn Thr Glu Pro Lys

675 680 685

His Cys Phe Lys Lys Gly Gly Gln His Ile Glu Val Trp Phe Asp Ala

690 695 700

Ala Ala Tyr Val Ala Trp Asp Ser Val Tyr Tyr Cys Gly Asp Asp Gly

705 710 715 720

Trp Cys Lys Thr Ala Ala Ala Glu Ala Glu Lys Tyr Gly Cys Lys Gly

725 730 735

Thr Trp Glu Val His Phe Gly Ala Ala Ala Asn Ser Ile Asp Cys Asn

740 745 750

Asp Ser Met Cys Ser Thr Phe Asp Asp Asn Val Ser Ala Thr Glu Leu

755 760 765

Val Lys Ala Ala Ala Asp His Ile Asp Tyr Trp Lys Leu Ile Arg Leu

770 775 780

Glu Cys Ala Ile Phe Tyr Lys Ala Arg Ala Ala Ala Arg Arg Leu Ser

785 790 795 800

Ser Asp Gln Asp Gln Ser Gln Ala Ala Ala Leu Val Thr Lys Tyr Pro

805 810 815

Leu Leu Lys Leu Leu Ser Ala Ala Ala Arg Pro Pro Asn Met Gly Val

820 825 830

Lys Ala His Gly Lys Cys Ile Trp Glu Asn Lys Val Phe Ile Val Pro

835 840 845

Thr Leu Cys Pro Val Pro Leu Asp Pro Thr Tyr Pro Leu Leu Lys Leu

850 855 860

Leu Thr Ala Ala Ala Thr Gln Thr Thr Thr Pro Glu Asn Thr Ser Leu

865 870 875 880

Val Glu Leu Arg Val Thr Thr Pro Lys Ser Thr Val Val Ile Arg Leu

885 890 895

His Leu Ala Ala Ala Thr Thr Arg Tyr Pro Leu Leu Ser Leu Leu Asn

900 905 910

Ser Tyr Ser Thr Pro Pro His Arg Ile Pro Ala Pro Cys Pro Trp Ala

915 920 925

Pro Gln Arg Pro Ala Ala Ala Pro Ile Pro Lys Pro Ser Pro Trp Ala

930 935 940

Pro Ala Ala Ala Arg Ile Pro Ala Pro Cys Pro Trp Ala Pro Ala Ala

945 950 955 960

Ala Pro Arg Pro Pro His Cys Pro Trp Val Pro Ala Ala Ala Pro Pro

965 970 975

Pro Pro Pro Arg Pro Trp Ala Pro Ala Ala Ala Cys Phe Leu Leu Cys

980 985 990

Phe Cys Val Leu Leu Cys Val Cys Leu Leu Ile Arg Pro Leu Leu Leu

995 1000 1005

Ser Val Ser Thr Tyr Ala Ala Ala Leu Arg Pro Leu Leu Leu Ser

1010 1015 1020

Ile Ser Val Tyr Ala Gln Val Leu Val Leu Val Leu Leu Leu Trp

1025 1030 1035

Val Ser Ile Gly Ser Ala Ala Ala Leu Leu Pro Ser Val Cys Met

1040 1045 1050

Cys Ala Tyr Ala Trp Val Leu Val Phe Val Tyr Ile Val Val Ile

1055 1060 1065

Thr Ser Pro Ala Thr Ala Ala Ala Ile Val Tyr Arg Asp Gly Asn

1070 1075 1080

Pro Tyr Ala Val Cys Asp Lys Cys Leu Lys Phe Tyr Ser Lys Ile

1085 1090 1095

Ser Glu Tyr Arg His Tyr Cys Tyr Ser Leu Tyr Gly Thr Thr Leu

1100 1105 1110

Glu Gln Gln Tyr Asn Lys Pro Leu Cys Asp Leu Leu Ile Arg Cys

1115 1120 1125

Ile Asn Ala Ala Ala Val Val Tyr Arg Asp Ser Ile Pro His Ala

1130 1135 1140

Ala Cys His Lys Cys Ile Asp Phe Tyr Ser Arg Ile Arg Glu Leu

1145 1150 1155

Arg His Tyr Ser Asp Ser Val Tyr Gly Asp Thr Leu Glu Lys Leu

1160 1165 1170

Thr Asn Thr Gly Leu Tyr Asn Leu Leu Ile Arg Cys Leu Arg Ala

1175 1180 1185

Ala Ala Val Phe Cys Lys Lys Ala Leu Thr Ala Ser Glu Val Tyr

1190 1195 1200

Asn Phe Ala Tyr Thr Asp Leu Arg Val Val Tyr Arg Asp Ala Ala

1205 1210 1215

Ala Ser Lys Val Arg Lys Leu Arg Tyr Tyr Asn Cys Ser Val Tyr

1220 1225 1230

Gly Ala Ser Leu Ala Ala Ala Val Tyr Cys Lys Gly Gln Leu Thr

1235 1240 1245

Glu Thr Glu Val Leu Asp Phe Ala Phe Thr Asp Leu Thr Ile Val

1250 1255 1260

Tyr Arg Asp Ala Ala Ala Ser Lys Val Ser Glu Phe Arg Trp Tyr

1265 1270 1275

Arg Tyr Ser Val Tyr Gly Thr Thr Leu Ala Ala Ala Cys Val Glu

1280 1285 1290

Cys Lys Lys Thr Leu Gln Arg Ser Glu Val Tyr Asp Ala Ala Ala

1295 1300 1305

Cys Gln Arg Pro Leu Cys Pro Gln Glu Lys Lys Arg His Val Asp

1310 1315 1320

Leu Asn Lys Arg Phe His Ala Ala Ala Thr Leu His Glu Tyr Met

1325 1330 1335

Leu Asp Leu Gln Pro Glu Thr Thr Asp Leu Tyr Cys Tyr Glu Gln

1340 1345 1350

Ala Ala Ala Pro Glu Thr Thr Asp Leu His Cys Tyr Glu Gln Leu

1355 1360 1365

Gly Asp Ser Ser Asp Glu Glu Asp Thr Gly Gly Leu Asp Ala Ala

1370 1375 1380

Ala Gly Asp Glu Asp Glu Asp Glu Val Asp His Leu Gln Glu Gln

1385 1390 1395

Pro Gln Gln Ala Arg Arg Asp Glu Gln His Pro Cys Tyr Leu Ile

1400 1405 1410

Glu Thr Gln Cys Cys Arg Cys Glu Ser Leu Val Ala Ala Ala Glu

1415 1420 1425

Glu Asn Asp Glu Ile Asp Gly Val Asn His Gln His Leu Pro Ala

1430 1435 1440

Arg Arg Ala Glu Pro Gln Arg His Thr Met Leu Cys Met Cys Cys

1445 1450 1455

Lys Cys Glu Ala Arg Ile Ala Gly Ser Gly Pro Gly Ala Ser Gly

1460 1465 1470

Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr Arg Thr

1475 1480 1485

<210> 77

<211> 20

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<220>

<221> 尚未归类的特征

<222> (11)..(11)

<223> Xaa可以是任何天然氨基酸

<400> 77

Asp Glu Asp Glu Asn Ala Ser Asp Thr Gly Xaa Asp Leu Val Asp Phe

1 5 10 15

Ile Asp Asn Ser

20

<210> 78

<211> 19

<212> PRT

<213> 人乳头瘤病毒16型

<400> 78

Asp Glu Asn Glu Asn Asp Ser Thr Gly Glu Asp Leu Val Asp Phe Ile

1 5 10 15

Val Asn Asp

<210> 79

<211> 20

<212> PRT

<213> 人乳头瘤病毒18型

<400> 79

Asp Glu Asp Glu Asn Ala Thr Asp Thr Gly Ser Asp Met Val Asp Phe

1 5 10 15

Ile Asp Thr Gln

20

<210> 80

<211> 20

<212> PRT

<213> 人乳头瘤病毒31型

<400> 80

Asp Glu Asn Glu Asp Ser Ser Asp Thr Gly Glu Asp Met Val Asp Phe

1 5 10 15

Ile Asp Asn Cys

20

<210> 81

<211> 20

<212> PRT

<213> 人乳头瘤病毒52型

<400> 81

Asp Glu Asp Glu Asn Ala Tyr Asp Ser Gly Thr Asp Leu Ile Asp Phe

1 5 10 15

Ile Asp Asp Ser

20

<210> 82

<211> 19

<212> PRT

<213> 人乳头瘤病毒53型

<400> 82

Asp Glu Thr Asp Glu Glu Ser Thr Glu Ser Asp Leu Asp Gly Phe Ile

1 5 10 15

Asp Asn Ser

<210> 83

<211> 20

<212> PRT

<213> 人乳头瘤病毒58型

<400> 83

Asp Glu Asp Glu Thr Ala Asp Asp Ser Gly Thr Asp Leu Ile Glu Phe

1 5 10 15

Ile Asp Asp Ser

20

<210> 84

<211> 15

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 84

Ala Gln Leu Ala Asp Val Asn Ser Asn Ala Ala Ala Phe Leu Lys

1 5 10 15

<210> 85

<211> 15

<212> PRT

<213> 人乳头瘤病毒16型

<400> 85

Ala Gln Leu Ala Asp Thr Asn Ser Asn Ala Ser Ala Phe Leu Lys

1 5 10 15

<210> 86

<211> 15

<212> PRT

<213> 人乳头瘤病毒18型

<400> 86

Ala Leu Leu Ala Asp Ser Asn Ser Asn Ala Ala Ala Phe Leu Lys

1 5 10 15

<210> 87

<211> 15

<212> PRT

<213> 人乳头瘤病毒31型

<400> 87

Ala Gln Leu Ala Asp Ser Asp Ser Asn Ala Cys Ala Phe Leu Lys

1 5 10 15

<210> 88

<211> 15

<212> PRT

<213> 人乳头瘤病毒52型

<400> 88

Ala Gln Leu Ala Asp Val Asn Ser Asn Ala Ala Ala Phe Leu Lys

1 5 10 15

<210> 89

<211> 15

<212> PRT

<213> 人乳头瘤病毒53型

<400> 89

Ala Gln Leu Ala Asp Val Asp Ser Asn Ala Gln Ala Phe Leu Lys

1 5 10 15

<210> 90

<211> 15

<212> PRT

<213> 人乳头瘤病毒58型

<400> 90

Ala Gln Leu Ala Asp Val Asn Ser Asn Ala Ala Ala Phe Leu Arg

1 5 10 15

<210> 91

<211> 20

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<220>

<221> 尚未归类的特征

<222> (4)..(4)

<223> Xaa可以是任何天然氨基酸

<400> 91

Asn Cys Leu Xaa Leu Tyr Gly Pro Ala Asn Thr Gly Lys Ser Tyr Phe

1 5 10 15

Gly Met Ser Leu

20

<210> 92

<211> 20

<212> PRT

<213> 人乳头瘤病毒16型

<400> 92

Asn Cys Ile Leu Leu Tyr Gly Ala Ala Asn Thr Gly Lys Ser Leu Phe

1 5 10 15

Gly Met Ser Leu

20

<210> 93

<211> 20

<212> PRT

<213> 人乳头瘤病毒18型

<400> 93

Asn Cys Leu Val Phe Cys Gly Pro Ala Asn Thr Gly Lys Ser Tyr Phe

1 5 10 15

Gly Met Ser Phe

20

<210> 94

<211> 20

<212> PRT

<213> 人乳头瘤病毒31型

<400> 94

Asn Cys Ile Leu Ile His Gly Ala Pro Asn Thr Gly Lys Ser Tyr Phe

1 5 10 15

Gly Met Ser Leu

20

<210> 95

<211> 20

<212> PRT

<213> 人乳头瘤病毒52型

<400> 95

Asn Cys Leu Val Leu Tyr Gly Pro Ala Asn Thr Gly Lys Ser Tyr Phe

1 5 10 15

Gly Met Ser Leu

20

<210> 96

<211> 20

<212> PRT

<213> 人乳头瘤病毒53型

<400> 96

Asn Cys Leu Val Ile Tyr Gly Pro Pro Asn Thr Gly Lys Ser Cys Gly

1 5 10 15

Ala Met Ser Leu

20

<210> 97

<211> 20

<212> PRT

<213> 人乳头瘤病毒58型

<400> 97

Ser Cys Met Leu Leu Cys Gly Pro Ala Asn Thr Gly Lys Ser Tyr Phe

1 5 10 15

Gly Met Ser Leu

20

<210> 98

<211> 20

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<220>

<221> 尚未归类的特征

<222> (11)..(11)

<223> Xaa可以是任何天然氨基酸

<400> 98

Asp Glu Asp Glu Asn Ala Ser Asp Thr Gly Xaa Asp Leu Val Asp Phe

1 5 10 15

Ile Asp Asn Ser

20

<210> 99

<211> 20

<212> PRT

<213> 人乳头瘤病毒16型

<400> 99

Asp Glu Asn Glu Asn Asp Ser Asp Thr Gly Glu Asp Leu Val Asp Phe

1 5 10 15

Ile Val Asn Asp

20

<210> 100

<211> 20

<212> PRT

<213> 人乳头瘤病毒18型

<400> 100

Asp Glu Asp Glu Asn Ala Thr Asp Thr Gly Ser Asp Met Val Asp Phe

1 5 10 15

Ile Asp Thr Gln

20

<210> 101

<211> 20

<212> PRT

<213> 人乳头瘤病毒31型

<400> 101

Asp Glu Asn Glu Asp Ser Ser Asp Thr Gly Glu Asp Met Val Asp Phe

1 5 10 15

Ile Asp Asn Cys

20

<210> 102

<211> 20

<212> PRT

<213> 人乳头瘤病毒52型

<400> 102

Asp Glu Asp Glu Asn Ala Tyr Asp Ser Gly Thr Asp Leu Ile Asp Phe

1 5 10 15

Ile Asp Asp Ser

20

<210> 103

<211> 19

<212> PRT

<213> 人乳头瘤病毒53型

<400> 103

Asp Glu Thr Asp Glu Glu Ser Thr Glu Ser Asp Leu Asp Gly Phe Ile

1 5 10 15

Asp Asn Ser

<210> 104

<211> 20

<212> PRT

<213> 人乳头瘤病毒58型

<400> 104

Asp Glu Asp Glu Thr Ala Asp Asp Ser Gly Thr Asp Leu Ile Glu Phe

1 5 10 15

Ile Asp Asp Ser

20

<210> 105

<211> 20

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<220>

<221> 尚未归类的特征

<222> (5)..(5)

<223> Xaa可以是任何天然氨基酸

<220>

<221> 尚未归类的特征

<222> (7)..(7)

<223> Xaa可以是任何天然氨基酸

<220>

<221> 尚未归类的特征

<222> (15)..(15)

<223> Xaa可以是任何天然氨基酸

<400> 105

Asp Glu Asp Glu Xaa Ala Xaa Asp Ser Gly Thr Asp Leu Ile Xaa Phe

1 5 10 15

Ile Asp Asp Ser

20

<210> 106

<211> 20

<212> PRT

<213> 人乳头瘤病毒52型

<400> 106

Asp Glu Asp Glu Asn Ala Tyr Asp Ser Gly Thr Asp Leu Ile Asp Phe

1 5 10 15

Ile Asp Asp Ser

20

<210> 107

<211> 20

<212> PRT

<213> 人乳头瘤病毒58型

<400> 107

Asp Glu Asp Glu Thr Ala Asp Asp Ser Gly Thr Asp Leu Ile Glu Phe

1 5 10 15

Ile Asp Asp Ser

20

<210> 108

<211> 15

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 108

Ala Gln Leu Ala Asp Ser Asp Ser Asn Ala Cys Ala Phe Leu Lys

1 5 10 15

<210> 109

<211> 15

<212> PRT

<213> 人乳头瘤病毒31型

<400> 109

Ala Gln Leu Ala Asp Ser Asp Ser Asn Ala Cys Ala Phe Leu Lys

1 5 10 15

<210> 110

<211> 15

<212> PRT

<213> 人乳头瘤病毒53型

<400> 110

Ala Gln Leu Ala Asp Val Asp Ser Asn Ala Gln Ala Phe Leu Lys

1 5 10 15

<210> 111

<211> 20

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 111

Asn Cys Ile Leu Leu Tyr Gly Ala Ala Asn Thr Gly Lys Ser Leu Phe

1 5 10 15

Gly Met Ser Leu

20

<210> 112

<211> 20

<212> PRT

<213> 人乳头瘤病毒16型

<400> 112

Asn Cys Ile Leu Leu Tyr Gly Ala Ala Asn Thr Gly Lys Ser Leu Phe

1 5 10 15

Gly Met Ser Leu

20

<210> 113

<211> 20

<212> PRT

<213> 人乳头瘤病毒31型

<400> 113

Asn Cys Ile Leu Ile His Gly Ala Pro Asn Thr Gly Lys Ser Tyr Phe

1 5 10 15

Gly Met Ser Leu

20

<210> 114

<211> 17

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 114

Trp Pro Tyr Leu His Ser Arg Leu Val Val Phe Thr Phe Pro Asn Pro

1 5 10 15

Phe

<210> 115

<211> 17

<212> PRT

<213> 人乳头瘤病毒16型

<400> 115

Trp Pro Tyr Leu His Asn Arg Leu Val Val Phe Thr Phe Pro Asn Glu

1 5 10 15

Phe

<210> 116

<211> 17

<212> PRT

<213> 人乳头瘤病毒31型

<400> 116

Trp Pro Tyr Leu His Ser Arg Leu Val Val Phe Thr Phe Pro Asn Pro

1 5 10 15

Phe

<210> 117

<211> 17

<212> PRT

<213> 人乳头瘤病毒52型

<400> 117

Trp Pro Tyr Leu His Ser Arg Leu Val Val Phe His Phe Lys Asn Pro

1 5 10 15

Phe

<210> 118

<211> 19

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<220>

<221> 尚未归类的特征

<222> (6)..(6)

<223> Xaa可以是任何天然氨基酸

<400> 118

Asp Glu Asn Glu Asn Xaa Ser Asp Thr Gly Glu Asp Met Val Asp Phe

1 5 10 15

Ile Asp Asn

<210> 119

<211> 19

<212> PRT

<213> 人乳头瘤病毒31型

<400> 119

Asp Glu Asn Glu Asp Ser Ser Asp Thr Gly Glu Asp Met Val Asp Phe

1 5 10 15

Ile Asp Asn

<210> 120

<211> 19

<212> PRT

<213> 人乳头瘤病毒16型

<400> 120

Asp Glu Asn Glu Asn Asp Ser Asp Thr Gly Glu Asp Leu Val Asp Phe

1 5 10 15

Ile Val Asn

<210> 121

<211> 19

<212> PRT

<213> 人乳头瘤病毒18型

<400> 121

Asp Glu Asp Glu Asn Ala Thr Asp Thr Gly Ser Asp Met Val Asp Phe

1 5 10 15

Ile Asp Thr

<210> 122

<211> 19

<212> PRT

<213> 人乳头瘤病毒53型

<400> 122

Asp Glu Thr Asp Glu Glu Ser Thr Glu Ser Asp Leu Asp Gly Phe Ile

1 5 10 15

Asp Asn Ser

<210> 123

<211> 15

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 123

Ala Gln Leu Ala Asp Val Asn Ser Asn Ala Ala Ala Phe Leu Lys

1 5 10 15

<210> 124

<211> 15

<212> PRT

<213> 人乳头瘤病毒52型

<400> 124

Ala Gln Leu Ala Asp Val Asn Ser Asn Ala Ala Ala Phe Leu Lys

1 5 10 15

<210> 125

<211> 15

<212> PRT

<213> 人乳头瘤病毒58型

<400> 125

Ala Gln Leu Ala Asp Val Asn Ser Asn Ala Ala Ala Phe Leu Arg

1 5 10 15

<210> 126

<211> 15

<212> PRT

<213> 人乳头瘤病毒18型

<400> 126

Ala Leu Leu Ala Asp Ser Asn Ser Asn Ala Ala Ala Phe Leu Lys

1 5 10 15

<210> 127

<211> 15

<212> PRT

<213> 人乳头瘤病毒16型

<400> 127

Ala Gln Leu Ala Asp Thr Asn Ser Asn Ala Ser Ala Phe Leu Lys

1 5 10 15

<210> 128

<211> 20

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 128

Asn Cys Leu Val Leu Cys Gly Pro Ala Asn Thr Gly Lys Ser Tyr Phe

1 5 10 15

Gly Met Ser Leu

20

<210> 129

<211> 20

<212> PRT

<213> 人乳头瘤病毒18型

<400> 129

Asn Cys Leu Val Phe Cys Gly Pro Ala Asn Thr Gly Lys Ser Tyr Phe

1 5 10 15

Gly Met Ser Phe

20

<210> 130

<211> 20

<212> PRT

<213> 人乳头瘤病毒52型

<400> 130

Asn Cys Leu Val Leu Tyr Gly Pro Ala Asn Thr Gly Lys Ser Tyr Phe

1 5 10 15

Gly Met Ser Leu

20

<210> 131

<211> 20

<212> PRT

<213> 人乳头瘤病毒58型

<400> 131

Ser Cys Met Leu Leu Cys Gly Pro Ala Asn Thr Gly Lys Ser Tyr Phe

1 5 10 15

Gly Met Ser Leu

20

<210> 132

<211> 20

<212> PRT

<213> 人乳头瘤病毒53型

<400> 132

Asn Cys Leu Val Ile Tyr Gly Pro Pro Asn Thr Gly Lys Ser Cys Phe

1 5 10 15

Ala Met Ser Leu

20

<210> 133

<211> 16

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 133

Trp Pro Tyr Leu Glu Ser Arg Thr Val Phe Glu Phe Pro Asn Ala Phe

1 5 10 15

<210> 134

<211> 17

<212> PRT

<213> 人乳头瘤病毒18型

<400> 134

Trp Pro Tyr Leu Glu Ser Arg Ile Thr Val Phe Glu Phe Pro Asn Ala

1 5 10 15

Phe

<210> 135

<211> 17

<212> PRT

<213> 人乳头瘤病毒58型

<400> 135

Trp Pro Tyr Leu His Ser Arg Leu Thr Val Phe Glu Phe Asn Asn Pro

1 5 10 15

Phe

<210> 136

<211> 17

<212> PRT

<213> 人乳头瘤病毒53型

<400> 136

Leu Arg Tyr Leu His Ser Arg Ile His Val Leu Gln Phe Leu Asn Pro

1 5 10 15

Phe

<210> 137

<211> 20

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 137

Asp His Ile Asp Tyr Trp Lys His Met Arg Leu Glu Cys Ala Ile Tyr

1 5 10 15

Tyr Lys Ala Arg

20

<210> 138

<211> 20

<212> PRT

<213> 人乳头瘤病毒16型

<400> 138

Asp His Ile Asp Tyr Trp Lys His Met Arg Leu Glu Cys Ala Ile Tyr

1 5 10 15

Tyr Lys Ala Arg

20

<210> 139

<211> 20

<212> PRT

<213> 人乳头瘤病毒18型

<400> 139

Ser Gln Ile Gln Tyr Trp Gln Leu Ile Arg Trp Glu Asn Ala Ile Phe

1 5 10 15

Phe Ala Ala Arg

20

<210> 140

<211> 20

<212> PRT

<213> 人乳头瘤病毒31型

<400> 140

Asp His Ile Asp Tyr Trp Lys His Ile Arg Leu Glu Cys Val Leu Met

1 5 10 15

Tyr Lys Ala Arg

20

<210> 141

<211> 20

<212> PRT

<213> 人乳头瘤病毒52型

<400> 141

Ala Gln Ile Glu His Trp Lys Leu Thr Arg Met Glu Cys Val Leu Phe

1 5 10 15

Tyr Lys Ala Lys

20

<210> 142

<211> 20

<212> PRT

<213> 人乳头瘤病毒53型

<400> 142

Asp His Ile Asp Tyr Trp Lys Ala Val Arg Gln Glu Asn Val Ile Tyr

1 5 10 15

Tyr Lys Ala Arg

20

<210> 143

<211> 20

<212> PRT

<213> 人乳头瘤病毒58型

<400> 143

Ser Gln Ile Glu His Trp Lys Leu Ile Arg Met Glu Cys Ala Ile Met

1 5 10 15

Tyr Thr Ala Arg

20

<210> 144

<211> 20

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 144

Asp His Ile Asp Tyr Trp Lys Leu Ile Arg Leu Glu Cys Ala Ile Phe

1 5 10 15

Tyr Lys Ala Arg

20

<210> 145

<211> 15

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<220>

<221> 尚未归类的特征

<222> (1)..(3)

<223> Xaa可以是任何天然氨基酸

<400> 145

Xaa Xaa Xaa Pro Ile Pro Pro Pro Cys Pro Trp Ala Pro Lys Lys

1 5 10 15

<210> 146

<211> 16

<212> PRT

<213> 人乳头瘤病毒16型

<400> 146

Thr Pro Pro Arg Pro Ile Pro Lys Pro Ser Pro Trp Ala Pro Lys Lys

1 5 10 15

<210> 147

<211> 16

<212> PRT

<213> 人乳头瘤病毒18型

<400> 147

Thr Pro Pro His Arg Ile Pro Ala Pro Cys Pro Trp Ala Pro Gln Arg

1 5 10 15

<210> 148

<211> 16

<212> PRT

<213> 人乳头瘤病毒31型

<400> 148

Thr Pro Pro His Arg Ile Pro Lys Pro Ala Pro Trp Ala Pro Val Lys

1 5 10 15

<210> 149

<211> 12

<212> PRT

<213> 人乳头瘤病毒52型

<400> 149

Pro Arg Pro Pro His Cys Pro Trp Val Pro Lys Thr

1 5 10

<210> 150

<211> 12

<212> PRT

<213> 人乳头瘤病毒53型

<400> 150

Pro Pro Pro Pro Pro Arg Pro Trp Ala Pro Thr Lys

1 5 10

<210> 151

<211> 5

<212> PRT

<213> 人乳头瘤病毒58型

<400> 151

Pro Pro Thr Thr Lys

1 5

<210> 152

<211> 10

<212> PRT

<213> 人工序列

<220>

<223> 共有序列

<400> 152

Pro Ile Pro Lys Pro Ser Pro Trp Ala Pro

1 5 10

<210> 153

<211> 10

<212> PRT

<213> 人乳头瘤病毒16型

<400> 153

Pro Ile Pro Lys Pro Ser Pro Trp Ala Pro

1 5 10

<210> 154

<211> 10

<212> PRT

<213> 人乳头瘤病毒31型

<400> 154

Arg Ile Pro Lys Pro Ala Pro Trp Ala Pro

1 5 10

<210> 155

<211> 10

<212> PRT

<213> 人乳头瘤病毒18型

<400> 155

Arg Ile Pro Ala Pro Cys Pro Trp Ala Pro

1 5 10

<210> 156

<211> 10

<212> PRT

<213> 人乳头瘤病毒52型

<400> 156

Pro Arg Pro Pro His Cys Pro Trp Val Pro

1 5 10

<210> 157

<211> 10

<212> PRT

<213> 人乳头瘤病毒53型

<400> 157

Pro Pro Pro Pro Pro Arg Pro Trp Ala Pro

1 5 10

<210> 158

<211> 9713

<212> DNA

<213> 人工序列

<220>

<223> 质粒构建体

<400> 158

agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 60

acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 120

tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 180

ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg 240

catgcatctg gaaacgggca tctccattta agactagatg ccacggggtt taaaatacta 300

atcatgacat tttgtagagc gtaattactt agtaaatccg ccgtactagg ttcatttcct 360

cctcgtttgg atctcacatc agaaattaaa ataatcttag aaggatgcag ttgttttttg 420

atggatcgta gatattcctc atcaacgaac cgagtcacta gagtcacatc acgcaatcca 480

tttaaaatag gatcatgatg gcggccgtca attagcatcc atttgatgat cactcctaaa 540

ttatagaaat gatctctcaa ataacgtata tgtgtaccgg gagcagatcc tatatacact 600

acggtggcac catctaatat accgtgtcgc tgtaacttac taagaaaaaa taattctcct 660

agtaatagtt ttaactgtcc ttgatacggt agtttttttg cgacctcatt tgcactttct 720

ggttcgtaat ctaactcatt atcaatttcc tcaaaataca taaacggttt atctaacgac 780

acaacatcca tttttaagta ttatattaaa atttaatcaa tgtttatttt tagtttttta 840

gataaaaaat ataatattat gagtcgatgt aacactttct acacaccgat tgatacatat 900

cattacctcc tattatctct atctcggttt cctcacccaa tcgtttagaa aaggaagcct 960

ccttaaagca tttcatacac acagcagtta gttttaccac catttcagat aatggaataa 1020

gattcaaaat attattaaac ggtttacgtt gaaatgtccc atcgagtgcg gctactataa 1080

ctatttttcc ttcgtttgcc atacagatcc tacgtactcg agcggccgct tatcaggtcc 1140

gggtggagtc caggcccagc agtggattgg ggataggctt gccagaggcg ccaggtccag 1200

agccggcgat tctggcctcg cacttgcagc acatacacag catggtgtgt ctctgaggct 1260

cggcccttct agcgggcaga tgctggtggt tcacgccgtc gatctcgtcg ttctcttcca 1320

ccagagattc gcatctgcag cactgtgtct cgatcagata gcaagggtgc tgttcgtccc 1380

gtctagcctg ctggggctgt tcctgcagat ggtccacttc gtcctcatcc tcgtccccat 1440

ccaggccgcc agtgtcctct tcatcggagc tgtctcccag ctgctcataa cagtgcagat 1500

cagtggtttc aggctgctcg tagcagtaca ggtcggttgt ctcgggctgc agatccagca 1560

tgtactcgtg cagggtgtgg aaccgcttgt tcaggtccac gtgtcttttc ttttcctgcg 1620

gacacagtgg ccgctggcag tcgtacacct cagatctctg cagggttttc ttgcattcca 1680

cgcacagtgt ggtgccatac acggaatatc tgtaccaccg gaactcggac accttggagt 1740

cgcgatacac gattgtcagg tctgtgaagg cgaaatccag cacctctgtc tcggtcagct 1800

gtcccttgca atacaccagg ctggcgccat acacagagca gttgtagtac ctcagcttcc 1860

gcactttgct gtcccgatac accacccgca gatcggtgta ggcgaagttg tacacctcgc 1920

tggctgtcag ggccttcttg cagaacaccc gcaggcatct aatcagcagg ttgtacaggc 1980

cagtgttggt cagcttttcc agggtatcgc cgtacacgga gtcgctgtag tgccgcagct 2040

ctctgattct ggagtagaag tcgatacact tgtggcaggc ggcgtggggg atggagtctc 2100

tgtacaccac gttgatgcac cgaatcagca gatcgcacag gggcttgttg tactgctgtt 2160

ccagggtggt gccgtacagg ctgtagcagt agtgccggta ctcgctgatc ttgctgtaga 2220

acttcaggca cttgtcgcac acggcgtaag gattgccatc ccggtacacg atggcggtgg 2280

cggggctggt aatcacgacg atgtacacga acaccagcac ccaggcatag gcacacatgc 2340

acacgctggg cagcaggctt ccgatggaca cccacagcag cagcaccagc accagcacct 2400

gagcgtacac gctgatagac agcagcagag gcctcaggta ggtggacaca ctcagcagca 2460

ggggtctgat cagcaggcac acgcacagca gcacacaaaa gcacagcagg aagcaagggg 2520

cccaaggtct tggaggaggt ggagggggca cccatggaca gtgtggaggt ctaggaggtg 2580

cccaggggca aggggcaggg attctagggg cccatggaga aggcttaggg atggggggcc 2640

tctgaggagc ccaaggacat ggagcaggga tccggtgagg gggggtgctg tagctgttca 2700

gcagtgacag cagtgggtat ctggtggtca ggtgcagcct gatcacgact gtgctcttgg 2760

gggtggtcac tctcagttcc accagggagg tattctcggg ggtggtggtc tgggtggtca 2820

gcagtttcag cagaggatat gttggatcca gaggcacggg gcacagggtg ggcacgatga 2880

acaccttgtt ctcccagatg cacttgccgt gggccttcac gcccatgttg gggggtctgg 2940

acagcagctt cagcaggggg tacttggtca cgagctgaga ctgatcctgg tcgctggaca 3000

gccgccgtct ggccttgtaa aaaatggcac attccaggcg aatcagcttc caatagtcga 3060

tatggtcctt cacgagctcg gtggcggaca cgttgtcgtc gaaggtgctg cacatggagt 3120

cgttgcagtc gatgctgttg ccgaaatgca cttcccaggt gcccttgcag ccgtacttct 3180

cggcctcggt cttgcaccag ccatcgtcgc cgcagtagta cacgctgtcc caggccacgt 3240

agtcgaacca cacttcgatg tgctggccgc ccttcttgaa gcagtgcttg ggctcggtgt 3300

tccactcgat ggctttacag gccttggcct tgcagatatt cagggcgggc accacctggt 3360

gtctggcggc gaagaagatg gcgttttcct gcctgatggc tttccaataa tcaatgtggt 3420

cggcttcctc ggtgctgatc tcatcggagg ggatgctggc ggggcacacg atcacgtaga 3480

tttctttcca atttgtgtaa tccatggtat tggccttgtc attgtcgtac tgcacggtga 3540

tggtgatgcc gtgcttctta aactggggct cggccagcca catttccagg ctggtctgct 3600

gcagggtcca ctcatcgtag gggctggcat tcagggcttc cagggccagc tgcagttcga 3660

tggcctggca ggctttggct ttagaggcgg ccagtggagg caccacctgg tggcagatgc 3720

tgatgcccag ctctttggcc ttatagaaga ttgcacattc catccggatc agtttccagt 3780

gctcgatctg ggcgttcagg tcgttcttgt cggcctcgta gtaggtcagg gtcacaatgg 3840

cgttcttgtg tttcccatcg tgacatgtcc aatgccactt gtgcttgccg tcgtggcagg 3900

tccagtgcca ggtgctgctc agcttgcagt gcttcttgaa tctgtaccgc aggcacttca 3960

ggatcagcag cttgttgggg tggcagttct cgcttctggg cctcttgatc cgccgctggc 4020

cgctctcagg acacaggatc acttggccgc cagcgtgcac ctcccatttc ttgtccacct 4080

ggccttccac cacggtgcac tgggcgtcct cgcagatata gatccagttg gtgtagtgca 4140

tggtgttgca gatgtcgccg tcgaactggt ggaagcccat ctcgcgagcc ttatacataa 4200

tagcacattc cagtctgatc cgggccttgt acatgatggc gcattccagc cggatgtgct 4260

tccagtagtc gatgtgatcg cagatgtggt cgcacaggtc cttggaatca ttctcataat 4320

gttccagaat gtccttgctg tcgttctcgt agtgttccag gatcttgtcc tggcacacgt 4380

tgaaggggtt cagaaactgc agcacgtgga tccgggagtg caggtatctc agaaaggcat 4440

tggggaactc gaacacggtg atccggcttt ccaggtaagg ccagaagggg ttggggaagg 4500

taaacaccac cagtctgctg tgcaggtagg gccacagaga catggcaaag caggacttgc 4560

ctgtgttagg tgggccgtag atcacgagac aattcaggga catgccgaag taggactttc 4620

cggtattggc tgggccgcac agcaccaggc agttcaggct catgccgaac aggctcttgc 4680

cggtgttggc agcgccgtac agcaggatgc agttcttcag aaaagcagcg gcgttgctgt 4740

tcacgtctgc cagctgagcc ttcaggaagg cgcaggcatt gctgtcgctg tcagccagct 4800

gggcggagtt gtcgataaag ccgtccaggt cgctctcggt gctctcttcg tcggtttcgt 4860

cgttgtcgat gaaatccacc atgtcctcgc cggtgtcgga gtcgttctca ttctcgtcgc 4920

tgtcgtcgat gaagtcgatc aggtcggtgc cgctgtcgta ggctgtctcg tcctcgtcgg 4980

ccagcttgct gcctcttctg aaccgggcgt ggatttcctg gctgggggac acaaacacgg 5040

cgccacacag cagcagcacg cagcacaggc ccctcttcat agcatccatg gtggcggcgc 5100

ggctagcggt accggatcta gatggggatc cgtcactgtt ctttatgatt ctacttcctt 5160

accgtgcaat aaattagaat atattttcta cttttacgag aaattaatta ttgtatttat 5220

tatttatggg tgaaaaactt actataaaaa gcgggtgggt ttggaattag tgatcagttt 5280

atgtatatcg caactaccgg gcatatggct atcgacatcg agaacattac ccacatgata 5340

agagattgta tcagtttcgt agtcttgagt attggtatta ctatatagta tatagatgtc 5400

gacctgcagg tcgacgaagt tcctatactt tctagagaat aggaacttcg cagccaagct 5460

ggaattcatc cactttggat aagaaatctg catgataaat atattgatat cctaccacct 5520

attaaagtac cattatctaa tagcaataag atagataaac aaatgttttt tgatgaagtt 5580

attacgtgga taaatatata tcttcaggaa aagggtatta tgttaccaga tgatataaga 5640

gaactcagag atgctattat tccttaacta gttacgtctc tttaggtact tattttgata 5700

cgttacaagt aaaaaactat caaatataaa tggaatctga ttctaatata gcgattgaag 5760

aggatccacc ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc 5820

ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg 5880

gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc 5940

tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc 6000

gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg 6060

tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga 6120

agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg 6180

acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca 6240

tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg 6300

acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg 6360

tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg 6420

agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca 6480

tggacgagct gtacaagtaa agcggccgcg aagttcctat actttctaga gaataggaac 6540

ttcaacaatg tctggaaaga actgtccttc atcgatacct atcacggaga aatctgtaat 6600

tgattccaag acatcacata gtttagttgc ttccaatgct tcaaaattat tcttatcatg 6660

cgtccatagt cccgttccgt atctattatc gttagaatat tttatagtca cgcatttata 6720

ttgagctatt tgataacgtc taactcgtct aattaattct gtacttttac ctgaaaacat 6780

ggggccgatt atcaactgaa tatgtccgcc gttcatgatg acaataaaga attaattatt 6840

gttcacttta ttcgacttta atatatccat cacgttagaa aatgcgatat cgcgacgagg 6900

atctatgtat ctaacaggat ctattgcggt ggtagctaga gctgattctt ttttgaatcg 6960

catcaaacta atcacaaagt cgaacaaata tcctttatta agtttgaccc ttccatctgt 7020

aacaataggg accttgttaa acagtttttt aaaatcttga gagtctgtga attttgtcaa 7080

ttgtctgtat tcctctgaaa gagattcata acaatgaccc acggcttcta atttattttt 7140

tgattggatc aataataata acagaaagtc tagatattga gtgatttgca atatatcaga 7200

taatgaagat tcatcatctt gactagccaa atacttaaaa aatgaatcat catctgcgaa 7260

gaacatcgtt aagagatact ggttgtgatc catttatgag ctcgcgaaag cttggcactg 7320

gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt 7380

gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct 7440

tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg 7500

catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc 7560

gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt 7620

ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag 7680

aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt 7740

ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga 7800

aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 7860

atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt 7920

caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct 7980

cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt 8040

tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt 8100

tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac 8160

gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac 8220

tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct 8280

gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg 8340

aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg 8400

gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca 8460

atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa 8520

caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt 8580

ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc 8640

attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg 8700

agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt 8760

aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt 8820

catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 8880

ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 8940

tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 9000

ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 9060

ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac 9120

ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 9180

gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 9240

aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 9300

acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 9360

gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 9420

gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 9480

cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 9540

aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct 9600

gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct 9660

cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga aga 9713

<210> 159

<211> 10405

<212> DNA

<213> 人工序列

<220>

<223> 质粒构建体

<400> 159

agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 60

acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 120

tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 180

ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat 240

ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca 300

ctagtaacgg ccgccagtgt gctggaattc gcccttgtaa tctattcgat ataccgttgc 360

taacagtata ctggcccaat aactgtggat ggaaaatcta taataataca ttaatatcat 420

ccgatggtgc tagggttatt tggatggatg cgtataaatt ttcttgcggt ttatctttac 480

aagactattg ttatcattgg ggtagcaaac cagagagccg accattcgat ttaataaaaa 540

aatcagatgc taaacgcaat tctaaatcgt tggtcaaaga atctatggca tccttgaaat 600

ccttgtacga ggcattcgag acacaatcag gagcgttaga agttttaatg agtccatgta 660

ggatgttttc gttttctaga atagaagaca tgttcttaac tagtgtcatt aatagagtat 720

ccgagaatac tggaatgggg atgtattatc ctaccaacga tataccttct ctatttatcg 780

aatcatctat ctgtctagat tatattatag taaataatca ggaatccaac aaatatcgta 840

tcaaatctgt tctcgatatc atttcttcaa aacaataccc tgcaggacgt cccaactacg 900

ttaaaaatgg tacaaaagga aagttatata tcgcgttgtg taaagttacc gtacctacta 960

acgaccatat tccagtagtt tatcacgatg atgacaatac taccaccttt attacagtat 1020

tgacgtccgt cgatattgaa actgctatca gagcaggata ttcgatagtc gaattagggg 1080

ctttacaatg ggataataat attccagaac ttaaaaacgg tttactggat agtatcaaga 1140

tgatttatga cttgaacgca gttacaacaa ataatttatt ggaacagctc atagaaaata 1200

ttaactttaa caactctagt ataatttcgt tgttttatac atttgccatt agttattgcc 1260

gagcattcat ttactcaatt atggaaacca tagatccggt gtatatatct cagttcagtt 1320

ataaagaatt atacgttagt agctcttata aagatattaa tgaatccatg agtcagatgg 1380

taaaattata aaaagtgaaa aacaatatta tttttatcgt tggttgttac actatggatg 1440

ctatgaagag gggcctgtgc tgcgtgctgc tgctgtgtgg cgccgtgttt gtgtccccca 1500

gccaggaaat ccacgcccgg ttcagaagag gcagcaagct ggccgacgag gacgagacag 1560

cctacgacag cggcaccgac ctgatcgact tcatcgacga cagcgacgag aatgagaacg 1620

actccgacac cggcgaggac atggtggatt tcatcgacaa cgacgaaacc gacgaagaga 1680

gcaccgagag cgacctggac ggctttatcg acaactccgc ccagctggct gacagcgaca 1740

gcaatgcctg cgccttcctg aaggctcagc tggcagacgt gaacagcaac gccgctgctt 1800

ttctgaagaa ctgcatcctg ctgtacggcg ctgccaacac cggcaagagc ctgttcggca 1860

tgagcctgaa ctgcctggtg ctgtgcggcc cagccaatac cggaaagtcc tacttcggca 1920

tgtccctgaa ttgtctcgtg atctacggcc cacctaacac aggcaagtcc tgctttgcca 1980

tgtctctgtg gccctacctg cacagcagac tggtggtgtt taccttcccc aaccccttct 2040

ggccttacct ggaaagccgg atcaccgtgt tcgagttccc caatgccttt ctgagatacc 2100

tgcactcccg gatccacgtg ctgcagtttc tgaacccctt caacgtgtgc caggacaaga 2160

tcctggaaca ctacgagaac gacagcaagg acattctgga acattatgag aatgattcca 2220

aggacctgtg cgaccacatc tgcgatcaca tcgactactg gaagcacatc cggctggaat 2280

gcgccatcat gtacaaggcc cggatcagac tggaatgtgc tattatgtat aaggctcgcg 2340

agatgggctt ccaccagttc gacggcgaca tctgcaacac catgcactac accaactgga 2400

tctatatctg cgaggacgcc cagtgcaccg tggtggaagg ccaggtggac aagaaatggg 2460

aggtgcacgc tggcggccaa gtgatcctgt gtcctgagag cggccagcgg cggatcaaga 2520

ggcccagaag cgagaactgc caccccaaca agctgctgat cctgaagtgc ctgcggtaca 2580

gattcaagaa gcactgcaag ctgagcagca cctggcactg gacctgccac gacggcaagc 2640

acaagtggca ttggacatgt cacgatggga aacacaagaa cgccattgtg accctgacct 2700

actacgaggc cgacaagaac gacctgaacg cccagatcga gcactggaaa ctgatccgga 2760

tggaatgtgc aatcttctat aaggccaaag agctgggcat cagcatctgc caccaggtgg 2820

tgcctccact ggccgcctct aaagccaaag cctgccaggc catcgaactg cagctggccc 2880

tggaagccct gaatgccagc ccctacgatg agtggaccct gcagcagacc agcctggaaa 2940

tgtggctggc cgagccccag tttaagaagc acggcatcac catcaccgtg cagtacgaca 3000

atgacaaggc caataccatg gattacacaa attggaaaga aatctacgtg atcgtgtgcc 3060

ccgccagcat cccctccgat gagatcagca ccgaggaagc cgaccacatt gattattgga 3120

aagccatcag gcaggaaaac gccatcttct tcgccgccag acaccaggtg gtgcccgccc 3180

tgaatatctg caaggccaag gcctgtaaag ccatcgagtg gaacaccgag cccaagcact 3240

gcttcaagaa gggcggccag cacatcgaag tgtggttcga ctacgtggcc tgggacagcg 3300

tgtactactg cggcgacgat ggctggtgca agaccgaggc cgagaagtac ggctgcaagg 3360

gcacctggga agtgcatttc ggcaacagca tcgactgcaa cgactccatg tgcagcacct 3420

tcgacgacaa cgtgtccgcc accgagctcg tgaaggacca tatcgactat tggaagctga 3480

ttcgcctgga atgtgccatt ttttacaagg ccagacggcg gctgtccagc gaccaggatc 3540

agtctcagct cgtgaccaag taccccctgc tgaagctgct gtccagaccc cccaacatgg 3600

gcgtgaaggc ccacggcaag tgcatctggg agaacaaggt gttcatcgtg cccaccctgt 3660

gccccgtgcc tctggatcca acatatcctc tgctgaaact gctgaccacc cagaccacca 3720

cccccgagaa tacctccctg gtggaactga gagtgaccac ccccaagagc acagtcgtga 3780

tcaggctgca cctgaccacc agatacccac tgctgtcact gctgaacagc tacagcaccc 3840

cccctcaccg gatccctgct ccatgtcctt gggctcctca gaggcccccc atccctaagc 3900

cttctccatg ggcccctaga atccctgccc cttgcccctg ggcacctcct agacctccac 3960

actgtccatg ggtgccccct ccacctcctc caagaccttg ggccccttgc ttcctgctgt 4020

gcttttgtgt gctgctgtgc gtgtgcctgc tgatcagacc cctgctgctg agtgtgtcca 4080

cctacctgag gcctctgctg ctgtctatca gcgtgtacgc tcaggtgctg gtgctggtgc 4140

tgctgctgtg ggtgtccatc ggaagcctgc tgcccagcgt gtgcatgtgt gcctatgcct 4200

gggtgctggt gttcgtgtac atcgtcgtga ttaccagccc cgccaccgcc atcgtgtacc 4260

gggatggcaa tccttacgcc gtgtgcgaca agtgcctgaa gttctacagc aagatcagcg 4320

agtaccggca ctactgctac agcctgtacg gcaccaccct ggaacagcag tacaacaagc 4380

ccctgtgcga tctgctgatt cggtgcatca acgtggtgta cagagactcc atcccccacg 4440

ccgcctgcca caagtgtatc gacttctact ccagaatcag agagctgcgg cactacagcg 4500

actccgtgta cggcgatacc ctggaaaagc tgaccaacac tggcctgtac aacctgctga 4560

ttagatgcct gcgggtgttc tgcaagaagg ccctgacagc cagcgaggtg tacaacttcg 4620

cctacaccga tctgcgggtg gtgtatcggg acagcaaagt gcggaagctg aggtactaca 4680

actgctctgt gtatggcgcc agcctggtgt attgcaaggg acagctgacc gagacagagg 4740

tgctggattt cgccttcaca gacctgacaa tcgtgtatcg cgactccaag gtgtccgagt 4800

tccggtggta cagatattcc gtgtatggca ccacactgtg cgtggaatgc aagaaaaccc 4860

tgcagagatc tgaggtgtac gactgccagc ggccactgtg tccgcaggaa aagaaaagac 4920

acgtggacct gaacaagcgg ttccacaccc tgcacgagta catgctggat ctgcagcccg 4980

agacaaccga cctgtactgc tacgagcagc ctgaaaccac tgatctgcac tgttatgagc 5040

agctgggaga cagctccgat gaagaggaca ctggcggcct ggatggggac gaggatgagg 5100

acgaagtgga ccatctgcag gaacagcccc agcaggctag acgggacgaa cagcaccctt 5160

gctatctgat cgagacacag tgctgcagat gcgaatctct ggtggaagag aacgacgaga 5220

tcgacggcgt gaaccaccag catctgcccg ctagaagggc cgagcctcag agacacacca 5280

tgctgtgtat gtgctgcaag tgcgaggcca gaatcgccgg ctaattttta taaccgagtt 5340

tctgcattat tgtaattcgt atgctggcac catcaaagaa tcacttctaa aagatatcaa 5400

tatcacacat acaaatatta ctaccctatt gaatgagaca gccaaggtta tcaagttagt 5460

aaaatctctg gtagataaag aagatactga tattgtgaat aatttcatta ccaaagaaat 5520

taaaaacaga gacaaaatag ttaatagttt gtctctatca aacctggact ttcgtttgta 5580

aattggggct ttttgtacaa taaatgggtg ttgccaatga ttcatcccct gaatatcaat 5640

ggatgtctcc ccatagatta tcagatactg ttatattagg agactgtttg tattttaaca 5700

atataatgtc ccaattagat ttacaccaaa attgggctcc atcagttaga ttgttaaatt 5760

attttaagaa ttttaataag gaaacactac taaagataga agagaatgat tacattaatt 5820

catccttttt ccaacaaaag gataaacgat tttatcctat aaacgacgat ttttatcaca 5880

tatctacagg aggatatggt atagtcttta agatagataa ctatgtagta aaatttgtat 5940

tcgaggccac aaaattatat agtcccatgg aaactacggc ggagttcaca gtacccaaat 6000

ttctatacaa caatctaaag ggagatgaaa aaaaattaat cgtgtgtgcg tgggccatgg 6060

gattaaacta taaattaaca tttttacata ctctgtataa acgtgttctt catatgttgc 6120

tattattgat acaaactatg gatggtcagg aactatcatt gagatattct tctaaagttt 6180

ttttaaaggc gtttaacgag agaaaggaca gtatcaaatt cgtgaaatta ctatcccact 6240

tttatccggc agttattaac agtaatatta atgttataaa ctattttaac cgcatgtttc 6300

actttttcga acatgaaaag agaactaact acgaatacga aagaggaaat attataattt 6360

ttcccctagc actgtattcg gcagataaag tagataccga gctagctatc aaattaggat 6420

ttaaatcttt ggtacaatac ataaagttta tctttttaca gatggctctg ttatacatta 6480

aaatttacga actaccatgc tgcgacaact ttttacacgc agatcttaaa cccgataata 6540

tcttactttt tgattccaat gaaccaataa taattcatct aaaggataaa aagtttgttt 6600

ttaatgaacg tattaaatcg gcattaaacg actttgactt ttcccaagaa gggcgaattc 6660

tgcagatatc catcacactg gcggccgctt acttgtacag ctcgtccatg ccgagagtga 6720

tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt 6780

tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc 6840

cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt 6900

ggcggatctt gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt 6960

tgtggctgtt gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt 7020

cgatgccctt cagctcgatg cggttcacca gggtatcgcc ctcgaacttc acctcggcgc 7080

gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg 7140

gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact 7200

gcacgccgta ggtcagggtg gtcacgaggg tcggccaggg cacgggcagc ttgccggtgg 7260

tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca 7320

cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg 7380

taaacagctc ctcgcccttg ctcaccatgt ttaaacttta tattccaaaa aaaaaaaata 7440

aaatttcaat ttttgtttaa acgttgtacg gcagtttaag gtttacacct ataaaagaga 7500

gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc cggggcgacg 7560

gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta 7620

cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt 7680

gccggtctcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa 7740

aaacgccatt aacctgatgt tctggggaat ataaatgtca ggcatgagat tatcaaaaag 7800

gatcttcacc tagatccttt tcacgtagaa agccagtccg cagaaacggt gctgaccccg 7860

gatgaatgtc agctactggg ctatctggac aagggaaaac gcaagcgcaa agagaaagca 7920

ggtagcttgc agtgggctta catggcgata gctagactgg gcggttttat ggacagcaag 7980

cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa 8040

ctggatggct ttctcgccgc caaggatctg atggcgcagg ggatcaagct ctgatcaaga 8100

gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc 8160

cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga 8220

tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct 8280

gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac 8340

gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct 8400

attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt 8460

atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt 8520

cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt 8580

cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag 8640

gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt 8700

gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg 8760

tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg 8820

cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg 8880

catcgccttc tatcgccttc ttgacgagtt cttctgaatt attaacgctt acaatttcct 8940

gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac aggtggcact 9000

tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg 9060

tatccgctca tgagacaata accctgataa atgcttcaat aatagcacgt gaggagggcc 9120

accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg 9180

gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc 9240

ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg 9300

gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg 9360

gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag 9420

cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg 9480

gccgaggagc aggactgaca cgtgctaaaa cttcattttt aatttaaaag gatctaggtg 9540

aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga 9600

gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta 9660

atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa 9720

gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact 9780

gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 9840

tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 9900

accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 9960

ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 10020

cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 10080

agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 10140

ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg 10200

tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctgggc 10260

ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac 10320

cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc 10380

gagtcagtga gcgaggaagc ggaag 10405

Claims (40)

1.一种核酸，其编码包含多个保守肽序列或其变体的多肽，

其中所述保守序列在一个或多个HPV基因型16、18、31、52、53和58中是保守的；并且

其中所述多肽包含HPV蛋白质E1、E2、E4、E5、E6和E7中的每一种的保守肽序列。

2.根据权利要求1所述的核酸，其中所述多肽是融合多肽。

3.根据权利要求1或权利要求2所述的核酸，其中所述多肽不包含完整的基因序列。

4.根据任一项前述权利要求所述的核酸，其中所述多肽基本上由保守肽序列、肽佐剂序列和其间的一个或多个接头组成。

5.根据任一项前述权利要求所述的核酸，其中所述多个保守肽序列包含10个或更多个保守肽序列。

6.根据任一项前述权利要求所述的核酸，其中所述多个保守肽序列包含40个或更多个保守肽序列。

7.根据任一项前述权利要求所述的核酸，其中经编码的多肽包含HPV蛋白质E1、E2、E4、E5、E6和E7中的每一种的至少3个不同的保守肽序列。

8.根据任一项前述权利要求所述的核酸，其中所述多个保守肽序列衍生自相对于彼此不同的序列区域并且在野生型中不天然地同时存在。

9.根据任一项前述权利要求所述的核酸，其中接头残基设置在一个或多个、或全部保守肽序列之间。

10.根据权利要求9所述的核酸，其中所述接头包含K、G、P或S氨基酸残基或其组合、或由以上组成。

11.根据任一项前述权利要求所述的核酸，其中所述保守肽序列选自以任何顺序包含SEQ ID NO:1至59；其变体或其组合的组中的任一种。

12.根据任一项前述权利要求所述的核酸，其中所述多肽包含选自SEQ ID NO:1-11中的任一种的一个或多个保守E1序列；选自SEQ ID NO:12-35中的任一种的一个或多个保守E2序列；选自SEQ ID NO:36-44中的任一种的一个或多个保守E4序列；选自SEQ ID NO:45-47中的任一种的一个或多个保守E5序列；选自SEQ ID NO:48-55中的任一种的一个或多个保守E6序列；以及选自SEQ ID NO:56-59中的任一种的一个或多个保守E7序列。

13.根据任一前述权利要求所述的核酸，其中所述多肽包含：

选自SEQ ID NO:1-11中的任一种的一个或多个保守E1序列，其中所述基因型16、18、31、52、53和58中的每一种由至少一个保守E1序列表示；

选自SEQ ID NO:12-35中的任一种的一个或多个保守E2序列，其中所述基因型16、18、31、52、53和58中的每一种由至少一个保守E2序列表示；

选自SEQ ID NO:36-44中的任一种的一个或多个保守E4序列，其中所述基因型16、18、31、52、53和58中的每一种由至少一个保守E4序列表示；

选自SEQ ID NO:45-47中的任一种的一个或多个保守E5序列，其中所述基因型16、18、31、52、53和58中的每一种由至少一个保守E5序列表示；

选自SEQ ID NO:48-55中的任一种的一个或多个保守E6序列，其中所述基因型16、18、31、52、53和58中的每一种由至少一个保守E6序列表示；和

选自SEQ ID NO:56-59中的任一种的一个或多个保守E7序列，其中所述基因型16、18、31、52、53和58中的每一种由至少一个保守E7序列表示。

14.根据任一前述权利要求所述的核酸，其中所述多肽包含：

选自SEQ ID NO:1-11中的任一种的一个或多个保守E1序列，其中所述基因型16、18、31、52和58中的每一种由至少一个保守E1序列表示；

选自SEQ ID NO:12-35中的任一种的一个或多个保守E2序列，其中所述基因型16、18、31、52和58中的每一种由至少一个保守E2序列表示；

选自SEQ ID NO:36-44中的任一种的一个或多个保守E4序列，其中所述基因型16、18、31、52和58中的每一种由至少一个保守E4序列表示；

选自SEQ ID NO:45-47中的任一种的一个或多个保守E5序列，其中所述基因型16、18、31、52和58中的每一种由至少一个保守E5序列表示；

选自SEQ ID NO:48-55中的任一种的一个或多个保守E6序列，其中所述基因型16、18、31、52和58中的每一种由至少一个保守E6序列表示；和

选自SEQ ID NO:56-59中的任一种的一个或多个保守E7序列，其中所述基因型16、18、31、52和58中的每一种由至少一个保守E7序列表示。

15.根据任一项前述权利要求所述的核酸，其中所述核酸包含序列SEQ ID NO:60或其变体、或由以上组成；或者

所述核酸包含序列SEQ ID NO:60或其变体、或由以上组成，并且不编码TPA前导序列；或者

所述核酸包含序列SEQ ID NO:60或其变体、或由以上组成，编码不同/替代的肽佐剂而非所述TPA前导序列；或者

所述核酸包含序列SEQ ID NO:65或其变体、或由以上组成。

16.根据任一项前述权利要求所述的核酸，其中所述核酸包含序列SEQ ID NO:62或其变体、或由以上组成；或者

所述核酸包含序列SEQ ID NO:62或其变体、或由以上组成，并且不编码TPA前导序列；或者

所述核酸包含序列SEQ ID NO:62或其变体、或由以上组成，编码不同/替代的肽佐剂而非所述TPA前导序列。

17.根据任一项前述权利要求所述的核酸，其中所述核酸包含序列SEQ ID NO:71、73或75或其变体、或由以上组成；或者

所述核酸包含序列SEQ ID NO:71、73或75或其变体、或由以上组成，并且不编码所述TPA前导序列；或者

所述核酸包含序列SEQ ID NO:71、73或75或其变体、或由以上组成，编码不同/替代的肽佐剂而非所述TPA前导序列。

71、73或75，

18.根据任一项前述权利要求所述的核酸，其中所述多肽包含序列SEQ ID NO:61或其变体、或由以上组成；

所述多肽包含序列SEQ ID NO:61或其变体、或由以上组成，并且不含所述TPA前导序列；或者

所述多肽包含序列SEQ ID NO:61或其变体、或由以上组成，具有不同/替代的肽佐剂而非所述TPA前导序列；或者

所述多肽包含序列SEQ ID NO:66或其变体、或由以上组成。

19.根据任一项前述权利要求所述的核酸，其中所述多肽包含序列SEQ ID NO:72、74或76或其变体、或由以上组成；

所述多肽包含序列SEQ ID NO:72、74或76或其变体、或由以上组成，并且不含所述TPA前导序列；或者

所述多肽包含序列SEQ ID NO:72、74或76或其变体、或由以上组成，具有不同/替代的肽佐剂而非所述TPA前导序列；或者

所述多肽包含序列SEQ ID NO:72、74或76或其变体、或由以上组成。

20.根据任一项前述权利要求所述的核酸，其中所述保守肽序列的长度是约7至250个氨基酸、或更多。

21.根据任一项前述权利要求所述的核酸，其中所述多肽进一步包含肽佐剂。

22.根据任一项前述权利要求所述的核酸，其中所述核酸是用于疫苗接种的质粒载体。

23.根据任一项前述权利要求所述的核酸，其中所述核酸包含病毒载体序列。

24.一种疫苗，其包含根据任一项前述权利要求所述的核酸。

25.根据权利要求24所述的疫苗，其包含根据权利要求1-20中任一项所述的编码不同多肽的不同核酸的组合。

26.根据权利要求24或25所述的疫苗，其进一步包含另一种治疗或预防活性成分。

27.根据权利要求24-26中任一项所述的疫苗，其还包含佐剂。

28.一种组合物，其包含多种根据权利要求1-23中任一项所述的不同核酸，任选地其中所述组合物是药学上可接受的组合物。

29.一种多肽，其由根据权利要求1-23中任一项所述的核酸编码。

30.一种病毒载体，其包含根据权利要求1-23中任一项所述的核酸。

31.根据权利要求30所述的病毒载体，其中所述病毒是腺病毒。

32.一种组合物，其包含以下中的一种或多种：

-根据权利要求1-23中任一项所述的核酸

-根据权利要求29所述的多肽；和/或

-根据权利要求30或31所述的病毒载体。

33.根据权利要求32所述的组合物，其中所述组合物在哺乳动物中是免疫原性的。

34.根据权利要求32或33所述的组合物，其进一步包含药学上可接受的载体。

35.根据权利要求1-23中任一项所述的核酸；根据权利要求24-27中任一项所述的疫苗；根据权利要求29所述的多肽；根据权利要求30或31所述的病毒载体；或根据权利要求28或32-34中任一项所述的组合物，其用于预防或治疗HPV感染。

36.一种治疗或预防HPV感染的方法，其包括施用根据权利要求1-23中任一项所述的核酸；根据权利要求24-27中任一项所述的疫苗；根据权利要求29所述的多肽；根据权利要求30或31所述的病毒载体；或根据权利要求28或32-34中任一项所述的组合物。

37.一种用于预防或治疗HPV感染的试剂，所述试剂包含根据权利要求1-23中任一项所述的核酸；根据权利要求29所述的多肽；根据权利要求30或31所述的病毒载体，或由其组成。

38.一种初免加强疫苗接种试剂盒，其包括：

-初免疫苗接种，所述初免疫苗接种包含根据权利要求1-23中任一项所述的核酸；根据权利要求24-27中任一项所述的疫苗；根据权利要求29所述的多肽；根据权利要求27或28所述的病毒载体；或根据权利要求28或32-34中任一项所述的组合物；和

-加强疫苗接种，所述加强疫苗接种包含根据权利要求1-23中任一项所述的核酸；根据权利要求24-27中任一项所述的疫苗；根据权利要求26所述的多肽；根据权利要求30或31所述的病毒载体；或根据权利要求28或32-34中任一项所述的组合物。

39.根据权利要求38所述的初免加强疫苗接种试剂盒，其中所述初免疫苗接种和加强疫苗接种是不同的。

40.根据权利要求39所述的初免加强疫苗接种试剂盒，其中所述初免疫苗接种和加强疫苗接种的不同在于编码的多肽序列和/或包含不同的病毒载体。

Images