CN111202165A

CN111202165A - Preparation for regulating balance of intestinal microorganisms of bees, preparation method and application thereof

Info

Publication number: CN111202165A
Application number: CN202010054853.9A
Authority: CN
Inventors: 赵喆
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-01-17
Filing date: 2020-01-17
Publication date: 2020-05-29

Abstract

The invention discloses a preparation for regulating the balance of bee intestinal microorganisms, which comprises the following components in parts by weight: 60-100 parts of sugar, 5-20 parts of fructo-oligosaccharide and 5-20 parts of probiotics, the product of the invention provides a new way for regulating bee intestinal microorganisms, can be used as a feed additive or a health product, and simultaneously provides a material basis for improving the quality of bee products, reducing drug residues and promoting the safety of human food.

Description

Preparation for regulating balance of intestinal microorganisms of bees, preparation method and application thereof

Technical Field

The invention relates to the technical field of biological preparations, in particular to a preparation for regulating the balance of bee intestinal microorganisms, a preparation method and application thereof.

Background

Disturbances of the intestinal flora can cause various diseases of bees, such as: sporozoosis of bee, spiroplasmosis (creeping bee disease), amebiasis (amoebiasis), paralysis, paratyphoid fever (diarrhea), and septicemia. The nosemosis of bees is caused by microsporidian of bees, and has short life cycle and rapid reproduction. Only infected adult bees, larvae and pupae do not cause diseases, the activity of bees is normal in the early stage of the disease, the later stage is cachectic and impatient, the abdomen is enlarged, the anterior dorsal plate and the abdominal apex become black, and the disease is dysentery and can not sting. Bee spiroplasmosis is a bee-forming disease caused by bee spiroplasmosis, and occurs in Europe, America and China, pathogenic bacteria are spiral filaments and mainly occur in early spring, are often complicated with bee spodosiasis and bee Martensis, the abdomen of a diseased bee expands, the disease is slow to move, wings do not sag and fly, the diseased bee can only crawl around a beehive, the midgut becomes white and swollen, the ring veins disappear, and the hindgut is filled with green water sample excrement. The causative agent of amoebic disease in bees is the bee Martianopsis variabilis, which belongs in the animal classification, phylum protozoa, subphylum trichuriforme, class prototheca. The amoeba has no telogen, only one nucleus. The pseudopodia is a classification characteristic of the pseudopodia and can change with different situations. When environmental factors are poor, cysts may form. The cysts are oval or spherical, the size is 6-7 microns, and the cysts can be discharged out of the body along with the feces of the sick bees. The bee Marsdenia tenacissima parasitizes in the Marsdenia tenacissima of the bee, breeds rapidly, can block the lumen of the Marsdenia tenacissima, destroy the normal excretory function of the Marsdenia tenacissima, make the sick bee very weak. The bee paralysis disease, also called as black bee disease and paralysis disease, is an infectious disease of adult bees caused by chronic paralysis virus or acute paralysis virus. The disease is quick in infection, serious in disease nature, relatively stubborn and difficult to treat, and has great harm to bees. If the bee is not prevented and treated in time, the honey is seriously reduced in yield if the bee is not prevented and treated in time, and bees die greatly if the bee is seriously killed. Bee paratyphoid is caused by bacillus veenhenius. Usually in the late winter and early spring, it can lead to death of adult bees due to diarrhea, so it is commonly called "diarrhea disease". Once this occurs, the safe overwintering and spring breeding of the bee colony is severely affected. Bee septicemia is an acute infectious disease caused by septicemia of bees. Bee septicemia mainly occurs in young bees, and after the disease occurs, the bees have symptoms of emotional upset, depression or indigestion, and finally refuse to eat food in severe cases. Finally, the ability of the people to move becomes weak, and the people cannot fly at all, except climbing out of the beehive, the people are expelled from the beehive by healthy bees in the beehive and climb near the hive entrance.

At present, medicines are mainly used for preventing and treating intestinal bacterial infection, but good prevention and treatment effects are not obtained due to inconsistent conditions. Among the selected medicines, metronidazole, moroxydine, terramycin, neomycin, chlortetracycline, compound sulfamethoxazole, chloramphenicol, sodium iodoaminothiazole or lincomycin and other medicines are still taken as main medicines, and the use of the medicines can generate medicine residues and influence the quality of bee products.

Disclosure of Invention

The invention aims to provide a preparation for regulating the balance of microorganisms in the intestinal tracts of bees, a preparation method and application thereof, so as to solve the problems in the background art.

In order to achieve the purpose, the invention provides the following technical scheme:

a preparation for regulating microorganism balance in bee intestinal tract comprises the following steps: the paint comprises the following components in parts by weight: 60-100 parts of sugar, 5-20 parts of fructo-oligosaccharide and 5-20 parts of probiotics.

As a further scheme of the invention: the paint also comprises the following components in parts by weight: 70-90 parts of sugar, 8-16 parts of fructo-oligosaccharide and 6-16 parts of probiotics.

As a further scheme of the invention: the paint also comprises the following components in parts by weight: 80 parts of sugar, 10 parts of fructo-oligosaccharide and 10 parts of probiotics.

As a further scheme of the invention: the paint also comprises the following components in parts by weight: 80 parts of sugar, 5 parts of fructo-oligosaccharide and 15 parts of probiotics.

A preparation method of a preparation for regulating the balance of intestinal microorganisms of bees comprises the following steps: uniformly stirring sugar, fructo-oligosaccharide and probiotics, and sealing to obtain the preparation for regulating bee intestinal microorganism balance.

As a further scheme of the invention: further comprising the steps of: the preparation for regulating the balance of the intestinal microorganisms of the bees is added into feed, sugar water and water of the bees or directly placed on a frame beam of the beehive.

The application of the preparation for regulating the intestinal microbial balance of bees is characterized in that 1g of the preparation for regulating the intestinal microbial balance of bees is fed to each splenic bee.

Compared with the prior art, the invention has the beneficial effects that: the preparation for regulating the bee intestinal microbial balance is used for feeding bees, regulating the bee intestinal flora balance, and has direct effects on food digestion, nutrition supply, disease and pest resistance, toxic component decomposition, growth promotion and the like of the bees.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The invention relates to a preparation for regulating the balance of bee intestinal microorganisms, which comprises the following components in parts by weight: 60-100 parts of sugar, 5-20 parts of fructo-oligosaccharide and 5-20 parts of probiotics.

The invention also provides application of the preparation for regulating the bee intestinal microbial balance, and the preparation for regulating the bee intestinal microbial balance is added into feed, sugar water and water of bees or directly placed on a frame beam of a beehive.

The preparation for regulating the bee intestinal microbial balance is used for feeding bees, regulating the bee intestinal flora balance, and has direct effects on food digestion, nutrition supply, disease and pest resistance, toxic component decomposition, growth promotion and the like of the bees.

The preparation for regulating the balance of the intestinal microorganisms of the bees, the preparation method and the application thereof provided by the embodiment of the invention are specifically explained below.

The invention provides a preparation for regulating the balance of bee intestinal microorganisms, which comprises the following components in parts by weight: 60-100 parts of sugar, 5-20 parts of fructo-oligosaccharide and 5-20 parts of probiotics.

Sugars primarily provide energy.

The fructo-oligosaccharide has the function of proliferating bifidobacteria in animal organisms, so that the growth rate of the bifidobacteria is increased, and harmful bacteria in intestinal tracts are inhibited to different degrees.

Probiotics are a class of active microorganisms that alter the composition of the flora in a portion of the host that are beneficial to the host. The intestinal health is maintained by regulating the immune function of host mucous membrane and system or by regulating the flora balance in intestinal tract, so that single microorganism or mixed microorganism with definite composition beneficial to the health of the host is generated.

Therefore, the embodiment of the invention provides a preparation for regulating bee intestinal microbial balance, which takes sugar, fructo-oligosaccharide and probiotics as main raw materials, is scientifically prepared according to a specific proportion, and provides a beneficial preparation for regulating bee intestinal flora balance by matching the effective components of the raw materials.

The embodiment of the invention also provides a preparation method of the preparation for regulating the balance of the intestinal microorganisms of the bees, which comprises the following steps:

mixing sugar and fructo-oligosaccharide, stirring, and sealing to obtain preparation for regulating balance of microorganism in bee intestinal tract.

The embodiment of the invention also provides application of the preparation for regulating the microbial balance of the bee intestinal tract, and the preparation for regulating the microbial balance of the bee intestinal tract is added into feed, sugar water and water of bees or directly placed on a frame beam of a beehive.

In some embodiments, the amount added is: 1g of preparation for regulating the balance of intestinal microorganisms of bees is fed to each splenic bee.

The composition for regulating the bee intestinal microorganism balance preparation comprises the following components in parts by weight:

the following experiments were carried out after the preparation according to the above method:

the control test was carried out for three months in 6 groups of 20 bees at random, and the test results were as follows: during control period, the preparation is fed 14 times, once per week, 100 g each time, directly spread on the frame beam, during which time sugar or honey is fed as usual, and the control group is fed with syrup or honey only.

The following table compares the formulations of the present invention with the control group.

Through the proportioning of the above examples, through comparative experiments, the effect of the use examples 1, 2 and 3 is obviously lower than that of example 4 after proportioning, and the effect of the use examples 5 and 6 is not as good as that of example 4 when the proportioning of fructo-oligosaccharide and probiotics is adjusted, so the example 4 with reasonable proportioning is selected.

The use effect is as follows: by comparison, we see that the number of swarms increased significantly more than the control group after continuous use of the preparation for regulating the balance of microorganisms in the bee intestinal tract. In addition, the control group of the bee colony of example 1 had creeping bee disease, the bee colony of example 2 had diarrhea, the bee colony of example 3 had chalkbrood disease, the bee colony of example 4 had paralysis, the bee colony of example 5 had snail mud eel body disease, the bee colony of example 6 had mites, and the test group of the bee colony did not show any occurrence of disease. Shows that the preparation for regulating the balance of the microorganisms in the bee intestinal tracts shows unique growth characteristics after entering the bee intestinal tracts, and generates organic acid and acetic acid, thereby inhibiting the growth of acid-sensitive pathogenic bacteria. The compound produced by the product can directly inhibit pathogenic bacteria, activate immune system of bee, and improve its disease resistance. After the product is used, microorganisms in the intestinal tracts of bees are in a balanced state, so that the health of the bees is promoted, bee diseases are prevented, normal bees are stronger, the health of the decayed bees is restored, and the colony vigor is strengthened; effectively preventing bee diseases and inhibiting mite damage; the service life of the bees is prolonged, and the acquisition capacity is improved; the reproductive capacity of the queen bee is improved, so that the queen bee can lay more eggs, and the egg laying area is enlarged.

The test results show that: the preparation is suitable for regulating the balance of the intestinal microorganisms of the bees, and the bees are healthier when the preparation is used.

It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.

Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims

1. The preparation for regulating the balance of the intestinal microorganisms of the bees is characterized by comprising the following components in parts by weight: 60-100 parts of sugar, 5-20 parts of fructo-oligosaccharide and 5-20 parts of probiotics.

2. The preparation for regulating the balance of microorganisms in the intestinal tracts of bees of claim 1, which further comprises the following components in parts by weight: 70-90 parts of sugar, 8-16 parts of fructo-oligosaccharide and 6-16 parts of probiotics.

3. The preparation for regulating the balance of microorganisms in the intestinal tracts of bees of claim 1, which further comprises the following components in parts by weight: 80 parts of sugar, 10 parts of fructo-oligosaccharide and 10 parts of probiotics.

4. The preparation for regulating the balance of microorganisms in the intestinal tracts of bees of claim 1, which further comprises the following components in parts by weight: 80 parts of sugar, 5 parts of fructo-oligosaccharide and 15 parts of probiotics.

5. A preparation method of a preparation for regulating the balance of intestinal microorganisms of bees is characterized by comprising the following steps: uniformly stirring sugar, fructo-oligosaccharide and probiotic bacteria, and sealing to obtain preparation for regulating bee intestinal microorganism balance.

6. The method for preparing the preparation for regulating the balance of microorganisms in the intestinal tracts of bees according to claim 5, further comprising the steps of: the preparation for regulating the balance of the intestinal microorganisms of the bees is added into feed, sugar water and water of the bees or directly placed on a frame beam of the beehive.

7. The application of the preparation for regulating the intestinal microbial balance of bees is characterized in that 1g of the preparation for regulating the intestinal microbial balance of bees is fed to each splenic bee.