CN111166820B - Traditional Chinese medicine composition containing fingered citron, preparation and application - Google Patents
Traditional Chinese medicine composition containing fingered citron, preparation and application Download PDFInfo
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Abstract
The invention belongs to the field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition containing fingered citron, which comprises the following raw materials in parts by weight: 3-5 parts of fingered citron, 3-5 parts of glossy privet fruit, 1-3 parts of Japanese thistle herb, 0.5-1.5 parts of Pu' er tea, 0.5-1.5 parts of chitosan, 2-4 parts of Chinese yam and 2-4 parts of rhizoma alismatis.
Description
Technical Field
The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition containing fingered citron, a preparation and application thereof.
Background
Fat accumulation is classified according to the quantity of qi and blood of skin and flesh of people in Huangdi's classic, and fat, paste and meat are classified, wherein the paste is slowly cooked in longitudinal and abdominal puffiness, the fat is firmly cooked in popliteal flesh, and the skin and flesh of meat people are not separated from the body and are large. The fat of the 'cream person' is accumulated in the abdomen, the body shape is small, the abdominal circumference is large, the grease is concentrated in the abdomen, the appearance and the fat amount of the abdomen are far larger than those of the fat person, and the fat-cream type abdominal obesity belongs to abdominal obesity. Abdominal obesity is generally fat accumulated in the peritoneum of the abdominal cavity and the abdominal cavity of organs, and is mainly surrounded by the subcutaneous and epididymis, and its appearance is mainly marked by a significant increase in waist circumference.
The pathogenesis of obesity is various, but the root cause of obesity is closely related to the life style of modern people, and adverse reactions such as overeating, smoking and alcoholism promote fat deposition and insulin resistance, so that the abdominal circumference is increased to cause abdominal obesity. Insulin resistance is the pathological basis of diabetes, and currently, insulin resistance is commonly used as insulin sensitizers, such as metformin and thiazolidinediones, but the medicines often have gastrointestinal reactions, anaphylactic reactions and the like. Thiazolidinedione drugs are not suitable for patients with absolute insulin deficiency, severe or acute heart failure, and have common side effects of headache, dizziness, hypodynamia, nausea, and liver injury of some patients. The traditional Chinese medicine has obvious curative effects on losing weight and reducing fat, and has the advantages of multiple components, multiple targets, multiple ways, lasting and mild property, less adverse reaction after long-term use and the like.
In the prior art, most weight-reducing medicines can cause diarrhea after being taken, and chitosan is a natural high molecular polymer and has good safety when being used as a natural fat adsorbent compared with other weight-reducing and lipid-lowering foods and medicines. However, the dosage of the chitosan is often required to be large in the aspect of reducing weight and fat, but constipation symptoms are easily caused by increasing the dosage, and the ideal effect cannot be achieved by excessively low dosage.
The fingered citron mainly contains chemical components such as volatile oil, flavonoid, polysaccharide and the like, has the effects of eliminating phlegm, relieving cough and asthma, and also has the effects of resisting inflammation, resisting bacteria, reducing blood pressure, resisting cancer and resisting depression. However, the fingered citron extract has low bioavailability and poor absorption, and the traditional Chinese medicine extract adopting water extraction is easy to hydrolyze or oxidize to generate hydrolysis or deterioration in the storage process.
Therefore, the search for a medicament which has definite curative effect, safety, reliability and good stability, can be used for losing weight and reducing fat for a long time and has the function of insulin resistance is very important.
Disclosure of Invention
The invention aims to provide the traditional Chinese medicine composition which has definite curative effect, safety, reliability and good stability, can be used for losing weight and reducing fat for a long time and has the insulin resistance effect.
In order to achieve the purpose, the invention adopts the following technical scheme:
a traditional Chinese medicine composition containing fingered citron comprises the following raw materials in parts by weight: 3-5 parts of fingered citron, 3-5 parts of glossy privet fruit, 1-3 parts of Japanese thistle herb, 0.5-1.5 parts of Pu' er tea, 0.5-1.5 parts of chitosan, 2-4 parts of Chinese yam and 2-4 parts of rhizoma alismatis.
Preferably, the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 5 parts of fingered citron, 3 parts of glossy privet fruit, 1 part of Japanese thistle herb, 0.5 part of Pu' er tea, 1 part of chitosan, 2 parts of Chinese yam and 2 parts of rhizoma alismatis.
The invention also provides a traditional Chinese medicine composition oral preparation containing fingered citron, which is prepared by taking water extracts or enzyme extracts of fingered citron, glossy privet fruit, Japanese thistle herb, Pu' er tea, chitosan, Chinese yam and oriental waterplantain rhizome as effective components and adding pharmaceutically acceptable auxiliary components.
Preferably, the oral preparation is decoction, extract, capsule, pill, mixture, tablet or granule. In the embodiment of the invention, the oral preparation can be decoction, and pharmaceutically acceptable auxiliary components can be added in other embodiments to prepare extract, capsule, pill, mixture, tablet or granule.
The invention also provides a preparation method of the fingered citron-containing traditional Chinese medicine composition oral preparation, and the oral preparation is prepared by the following steps:
(1) weighing fingered citron, glossy privet fruit, Japanese thistle herb, Pu' er tea, Chinese yam and rhizoma alismatis in parts by weight according to a formula, crushing, adding 10 times of distilled water by volume, decocting for 30-45 min, filtering, adding 8 times of distilled water by volume into filter residues, decocting for 30-45 min, filtering, and combining the filtrate to obtain a mixed solution A;
(2) weighing chitosan according to a formula, adding 0.5% trichoderma viride with the same volume as the mixed solution A in the step (1), stirring for dissolving, soaking for 2 hours, adding the extracting solution in the step (1), and standing for 3-6 hours to obtain a mixed solution B;
(3) concentrating the mixed B obtained in the step (2) to 1g/mL, adding pharmaceutically acceptable auxiliary components, and preparing into oral preparation.
In addition, the invention also provides application of the fingered citron-containing traditional Chinese medicine composition in preparing medicines for losing weight, reducing fat and improving insulin resistance.
The fingered citron extract has low bioavailability and poor absorption, and the fingered citron extract which is extracted by water is easy to hydrolyze or oxidize to generate hydrolysis or deterioration in the storage process.
Furthermore, the stability of the traditional Chinese medicine composition is proved by the stability of the test example 1, the stability of the traditional Chinese medicine composition is good, and the pharmacodynamics experiment of the test example 2 proves that the traditional Chinese medicine composition has obvious treatment effect on insulin resistance and obesity, and has no obvious toxic or side effect.
Compared with the prior art, the invention has the following advantages: 1) the traditional Chinese medicine composition has the advantages of good stability, low price of medicinal materials, wide sources and simple extraction process; 2) the traditional Chinese medicine composition is a natural medicine extract, has simple medicine composition and small administration dosage, has obvious treatment effect on insulin resistance and obesity through pharmacodynamic experiment verification, and has no obvious toxic or side effect; 3) the traditional Chinese medicine composition has the liver protection effect.
Detailed Description
The present invention will be described in further detail with reference to the following examples. It should not be understood that the scope of the above-described subject matter of the present invention is limited to the following examples.
Example 1Preparation of Chinese medicinal decoction
Weighing 4 parts of fingered citron, 5 parts of glossy privet fruit, 1 part of Japanese thistle herb, 1.5 parts of Pu' er tea, 1.5 parts of chitosan, 4 parts of Chinese yam and 3 parts of rhizoma alismatis; the preparation method comprises the following steps:
(1) weighing fingered citron, glossy privet fruit, Japanese thistle herb, Pu' er tea, Chinese yam and rhizoma alismatis in parts by weight according to a formula, crushing, adding 10 times of distilled water by volume, decocting for 30min, filtering, adding 8 times of distilled water by volume into filter residues, decocting for 30-45 min, filtering, and combining the filtrates to obtain a mixed solution A;
(2) weighing chitosan according to a formula, adding 0.5% trichoderma viride with the same volume as the mixed solution A in the step (1), stirring for dissolving, soaking for 2 hours, adding the extracting solution in the step (1), and standing for 4 hours to obtain a mixed solution B;
(3) and (3) concentrating the mixed B obtained in the step (2) to 1g/mL, and preparing into decoction.
Example 2Preparation of Chinese medicinal decoction
Taking 5 parts of fingered citron, 3 parts of glossy privet fruit, 1 part of Japanese thistle herb, 0.5 part of Pu' er tea, 1 part of chitosan, 2 parts of Chinese yam and 2 parts of rhizoma alismatis; the preparation method comprises the following steps:
(1) weighing fingered citron, glossy privet fruit, Japanese thistle herb, Pu' er tea, Chinese yam and rhizoma alismatis in parts by weight according to a formula, crushing, adding 10 times of distilled water by volume, decocting for 30-45 min, filtering, adding 8 times of distilled water by volume into filter residues, decocting for 40min, filtering, and combining the filtrates to obtain a mixed solution A;
(2) weighing chitosan according to a formula, adding 0.5% trichoderma viride with the same volume as the mixed solution A in the step (1), stirring for dissolving, soaking for 2 hours, adding the extracting solution in the step (1), standing for 3 hours, and filtering to obtain a mixed solution B;
(3) and (3) concentrating the mixed B obtained in the step (2) to 1g/mL, and preparing into decoction.
Example 3Preparation of Chinese medicinal decoction
Taking 3 parts of fingered citron, 3 parts of glossy privet fruit, 2 parts of Japanese thistle herb, 1 part of Pu' er tea, 0.5 part of chitosan, 3 parts of Chinese yam and 3 parts of rhizoma alismatis; the preparation method comprises the following steps:
(1) weighing fingered citron, glossy privet fruit, Japanese thistle herb, Pu' er tea, Chinese yam and rhizoma alismatis in parts by weight according to a formula, crushing, adding 10 times of distilled water by volume, decocting for 45min, filtering, adding 8 times of distilled water by volume into filter residues, decocting for 30-45 min, filtering, and combining the filtrates to obtain a mixed solution A;
(2) weighing chitosan according to a formula, adding 0.5% trichoderma viride with the same volume as the mixed solution A in the step (1), stirring for dissolving, soaking for 2 hours, adding the extracting solution in the step (1), standing for 4.5 hours, and filtering to obtain a mixed solution B;
(3) and (3) concentrating the mixed B obtained in the step (2) to 1g/mL, and preparing into decoction.
Comparative example 1Preparation of Chinese medicinal decoction
The difference from example 2 is that chitosan is not contained, the chitosan missing part is replaced by cationic starch, the preparation method is different from example 2 in that no chitosan bulk drug is added, and the rest steps are the same as example 2.
Comparative example 2Preparation of Chinese medicinal decoction
Taking 3 parts of glossy privet fruit, 1 part of Japanese thistle herb, 0.5 part of Pu' er tea, 1 part of chitosan, 4.5 parts of Chinese yam and 4.5 parts of rhizoma alismatis; the preparation method is the same as in example 2.
Comparative example 3Preparation of Chinese medicinal decoction
Taking 5 parts of fingered citron, 3 parts of glossy privet fruit, 1 part of Japanese thistle herb, 0.5 part of Pu' er tea, 1 part of chitosan and 4 parts of rhizoma alismatis; the preparation method is the same as in example 2.
Comparative example 4Preparation of Chinese medicinal decoction
Taking 5 parts of fingered citron, 3 parts of glossy privet fruit, 1 part of Japanese thistle herb, 0.5 part of Pu' er tea, 1 part of chitosan and 4 parts of Chinese yam; the preparation method is the same as in example 2.
Test example 1 stability test
Reagent: the traditional Chinese medicine decoction prepared in examples 1-3 and comparative examples 1, 3 and 4.
The method comprises the following steps: placing the decoctions in the same open air, standing at room temperature for 30 days, and detecting the content of fructus Citri Sarcodactylis extract (calculated as hesperidin) in each group at days 1, 5, 10, 20, and 30.
TABLE 1 Fingered citron extract content
As can be seen from table 1, in the test periods of days 1, 10, 20 and 30, compared with the comparative example group, after 30 days of the groups of examples 1-3, the content of the fingered citron extract exceeds 52%, which indicates that the chitosan, the yam and the rhizoma alismatis can significantly improve the stability of the fingered citron extract in the air.
Test example 2 pharmacodynamic test
1. Experimental reagent
Reagent: the traditional Chinese medicine decoction prepared in the examples 1-3 and the comparative examples 1-4; oselta capsules (batch No. 180401), Chongqing Huasen pharmaceutical Co., Ltd; glucose (GLU) assay kit (batch No. 20181108137), Shanghai Rongsheng biopharmaceuticals Co., Ltd; insulin (INS) assay kit (Lot: HY0587R1), Shanghai enzyme research Biotech, Inc.; high density lipoprotein cholesterol (HDL-C) assay kit (batch No. 20180305), low density lipoprotein cholesterol (LDL-C) assay kit (batch No. 20180305), Total Cholesterol (TC) assay kit (batch No. 20180315), Triglyceride (TG) assay kit (batch No. 20180915), alanine Aminotransferase (ALT) assay kit (batch No. 20181010) and aspartate Aminotransferase (AST) assay kit (batch No. 20181011) were purchased from Nanjing institute for bioengineering.
2. Experimental animal, model making and dosage
Animals: SD rats, SPF grade, male, body weight (200. + -. 20) g. Purchased from the experimental animals center of Zhongshan university, animal license number: SCXK (Yue) 2016-.
The random weighing was divided into blank, model, positive control and dosing groups, and the experiment was started after one week of adaptive feeding. The model group, the positive control group and the administration group were subjected to high fat diet molding for 10 weeks, except for the blank group to which an equal volume of physiological saline was administered.
Administration dose:
normal group: equal volume of normal saline
Model group: equal volume of normal saline
Positive control group: 75mg/kg orlistat
Example 1: taking the decoction prepared in example 1, and administering the decoction at a dose of 5.4g/kg
Example 2: taking the decoction prepared in example 2, and administering the decoction at a dose of 5.4g/kg
Example 3: taking the decoction prepared in example 3, and administering the decoction at a dose of 5.4g/kg
Example 3: taking the decoction prepared in example 3, and administering the decoction at a dose of 2.7g/kg
Example 3: taking the decoction prepared in example 3, and administering the decoction at a dose of 10.8g/kg
Comparative example 1: taking the decoction prepared in comparative example 1, and administering the dosage of 5.4g/kg
Comparative example 2: taking the decoction prepared in comparative example 2, and administering the dosage of 5.4g/kg
Comparative example 3: taking the decoction prepared in comparative example 3, and administering the dosage of 5.4g/kg
Comparative example 4: taking the decoction prepared in comparative example 4, and administering the dosage of 5.4g/kg
3. Method of producing a composite material
After the model building is successful, the gavage is administrated according to the dose, the continuous 6 weeks are carried out, and the water is not forbidden in 12 hours before the last day of the administration. 6h after the last administration, all rats are bled from the retroorbital venous plexus, after being placed at room temperature for 2h, serum is centrifugally separated at 4000r/min and 15min, animals are sacrificed, livers and subcutaneous fat are taken, the weight, waist circumference and TG, TC, LDL-C, HDL-C, GLU and insulin levels in the serum of the rats are measured, insulin resistance indexes are calculated, and liver lipid deposition and liver functions are observed. Fat index (%) ═ fat mass/body mass × 100%; liver index (%) ═ liver mass/body mass × 100%.
4. Results of the experiment
4.1 Effect of Chinese medicinal composition on rat body weight, waist circumference and feces
As shown in table 2, compared with the traditional Chinese medicine composition lacking any one of the components of fingered citron, chitosan, Chinese yam and rhizoma alismatis, the traditional Chinese medicine composition containing fingered citron, chitosan, Chinese yam and rhizoma alismatis can significantly reduce the weight and waist circumference of SD rats, and defecation is normal, while the weight and waist circumference improvement effect of rats lacking any one of the components of fingered citron, chitosan, Chinese yam and rhizoma alismatis is poor, or constipation or diarrhea occurs, which indicates that the fingered citron, chitosan, Chinese yam and rhizoma alismatis have the effect of synergistically reducing the weight and waist circumference of SD rats and have a protection effect on gastrointestinal tracts.
TABLE 2 Effect on rat body weight and waist circumference: (
n＝10)
Note: in comparison with the normal group,*P＜0.05，**p is less than 0.01; in comparison to the set of models,#P＜0.05，##P＜0.01。
4.2 Effect of Chinese medicinal composition on rat blood lipid level
As shown in Table 3, the results show that the traditional Chinese medicine composition containing fingered citron, Chinese yam and rhizoma alismatis can remarkably reduce TG, TC and LDL-C levels of SD rats and remarkably increase HDL-C levels, the effect is remarkably superior to that of the traditional Chinese medicine composition lacking fingered citron, Chinese yam and rhizoma alismatis, and the fingered citron, Chinese yam and rhizoma alismatis and other components in the traditional Chinese medicine composition can synergistically regulate the blood lipid level of obese rats.
TABLE 3 Effect on rat blood lipid levels
n＝10)
Note: in comparison with the normal group,*P＜0.05，**p is less than 0.01; in comparison to the set of models,#P＜0.05，##P＜0.01。
4.3 Effect of Chinese medicinal composition on rat blood sugar, insulin level and insulin resistance index
As shown in Table 4, the traditional Chinese medicine composition containing fingered citron, Chinese yam and rhizoma alismatis simultaneously disclosed by the invention can be used for remarkably reducing the fasting blood glucose, insulin and insulin resistance index level of obese SD rats, and the effect is superior to that of the traditional Chinese medicine composition without any component, so that the fingered citron, the Chinese yam and the rhizoma alismatis and other components of the traditional Chinese medicine composition have the effect of synergistically improving the insulin resistance of the obese SD rats.
TABLE 4 Effect on fasting plasma glucose, insulin and insulin resistance index in rats: (
n＝10)
Note: in comparison with the normal group,*P＜0.05，**p is less than 0.01; in comparison to the set of models,#P＜0.05，##P＜0.01。
4.4 Effect of Chinese medicinal composition on rat liver function
As shown in tables 5-7, compared with the model group, the traditional Chinese medicine composition containing fingered citron, Chinese yam and rhizoma alismatis can reduce ALT and AST levels (p is less than 0.05 or 0.01) of livers of obese rats, TG and TC levels of livers of rats and reduce liver indexes, and the effect is superior to that of the traditional Chinese medicine composition without any component, so that the traditional Chinese medicine composition containing fingered citron, Chinese yam and rhizoma alismatis has a protective effect on livers.
Table 5 pairs of ratsEffects of liver function: (
n＝10)
Note: in comparison with the normal group,**p is less than 0.01; in comparison to the set of models,#P＜0.05，##P＜0.01。
TABLE 6 Effect on rat liver lipid content: (
n＝10)
Note: in comparison with the normal group,*P＜0.05，**p is less than 0.01; in comparison to the set of models,#P＜0.05。
table 7 effects on rat muscle, fat, liver weight: (
n＝10)
Note: in comparison with the normal group,*P＜0.05，**p is less than 0.01; in comparison to the set of models,#P＜0.05。
the foregoing embodiments are merely illustrative of the principles and utilities of the present invention and are not intended to limit the invention. Any person skilled in the art can modify or change the above-mentioned embodiments without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which can be made by those skilled in the art without departing from the spirit and technical spirit of the present invention be covered by the claims of the present invention.
Claims (5)
1. The traditional Chinese medicine composition containing fingered citron is characterized by comprising the following raw materials in parts by weight: 3-5 parts of fingered citron, 3-5 parts of glossy privet fruit, 1-3 parts of Japanese thistle herb, 0.5-1.5 parts of Pu' er tea, 0.5-1.5 parts of chitosan, 2-4 parts of Chinese yam and 2-4 parts of rhizoma alismatis.
2. The fingered citron-containing traditional Chinese medicine composition as claimed in claim 1, wherein the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 5 parts of fingered citron, 3 parts of glossy privet fruit, 1 part of Japanese thistle herb, 0.5 part of Pu' er tea, 1 part of chitosan, 2 parts of Chinese yam and 2 parts of rhizoma alismatis.
3. The application of the fingered citron-containing traditional Chinese medicine composition oral preparation of claim 1 or 2 is characterized in that the oral preparation is prepared by the following steps:
(1) weighing fingered citron, glossy privet fruit, Japanese thistle herb, Pu' er tea, Chinese yam and rhizoma alismatis in parts by weight according to a formula, crushing, adding 10 times of distilled water by volume, decocting for 30-45 min, filtering, adding 8 times of distilled water by volume into filter residues, decocting for 30-45 min, filtering, and combining the filtrate to obtain a mixed solution A;
(2) weighing chitosan according to a formula, adding 0.5% trichoderma viride with the same volume as the mixed solution A in the step (1), stirring for dissolving, soaking for 2 hours, adding the extracting solution in the step (1), and standing for 3-6 hours to obtain a mixed solution B;
(3) concentrating the mixed B obtained in the step (2) to 1g/mL, adding pharmaceutically acceptable auxiliary components, and preparing into oral preparation.
4. The fingered citron-containing traditional Chinese medicine composition oral preparation as claimed in claim 3, wherein the oral preparation is decoction, extract, capsule, pill, mixture, tablet or granule.
5. The use of the fingered citron-containing traditional Chinese medicine composition according to any one of claims 1 to 2 or the fingered citron-containing composition oral preparation according to claim 3 in the preparation of medicines for losing weight and reducing fat and improving insulin resistance.
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| CN1628786A (en) * | 2004-08-30 | 2005-06-22 | 广东药学院 | Medicine for treating hyperlipemia |
| WO2011140638A1 (en) * | 2010-05-10 | 2011-11-17 | Corporation De L'ecole Polytechnique De Montreal | Gene therapy for diabetes with chitosan-delivered plasmid encoding glucagon-like peptide 1 |
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| CN108785549A (en) * | 2017-05-04 | 2018-11-13 | 王丽敏 | A kind of Chinese medicine preparation and preparation method for treating diabetes |
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|---|---|---|---|---|
| CN1628786A (en) * | 2004-08-30 | 2005-06-22 | 广东药学院 | Medicine for treating hyperlipemia |
| WO2011140638A1 (en) * | 2010-05-10 | 2011-11-17 | Corporation De L'ecole Polytechnique De Montreal | Gene therapy for diabetes with chitosan-delivered plasmid encoding glucagon-like peptide 1 |
| CN106177440A (en) * | 2016-08-18 | 2016-12-07 | 宿松县众创茶业种植专业合作社 | A kind of blood fat reducing Folium Camelliae sinensis and preparation method thereof |
| CN108785549A (en) * | 2017-05-04 | 2018-11-13 | 王丽敏 | A kind of Chinese medicine preparation and preparation method for treating diabetes |
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