CN111164485B - 用于光学检查多个显微样品的方法和装置 - Google Patents

用于光学检查多个显微样品的方法和装置 Download PDF

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Publication number
CN111164485B
CN111164485B CN201880063710.5A CN201880063710A CN111164485B CN 111164485 B CN111164485 B CN 111164485B CN 201880063710 A CN201880063710 A CN 201880063710A CN 111164485 B CN111164485 B CN 111164485B
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light
sample
light sheet
channel
samples
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CN111164485A (zh
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T.卡尔克布伦纳
J.西本摩根
T.奥特
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Carl Zeiss Microscopy GmbH
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Carl Zeiss Microscopy GmbH
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    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
    • G01N15/147—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1484—Optical investigation techniques, e.g. flow cytometry microstructural devices
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G02B21/08—Condensers
    • G02B21/082—Condensers for incident illumination only
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G01N2015/144—Imaging characterised by its optical setup

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  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Optics & Photonics (AREA)
  • Engineering & Computer Science (AREA)
  • Multimedia (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Microscoopes, Condenser (AREA)
CN201880063710.5A 2017-09-29 2018-09-25 用于光学检查多个显微样品的方法和装置 Active CN111164485B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102017122718.0 2017-09-29
DE102017122718.0A DE102017122718A1 (de) 2017-09-29 2017-09-29 Verfahren und Vorrichtung zur optischen Untersuchung einer Vielzahl mikroskopischer Proben
PCT/EP2018/075949 WO2019063539A1 (de) 2017-09-29 2018-09-25 Verfahren und vorrichtung zur optischen untersuchung einer vielzahl mikroskopischer proben

Publications (2)

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CN111164485A CN111164485A (zh) 2020-05-15
CN111164485B true CN111164485B (zh) 2023-01-24

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US (1) US11371927B2 (https=)
JP (1) JP7309698B2 (https=)
CN (1) CN111164485B (https=)
DE (1) DE102017122718A1 (https=)
WO (1) WO2019063539A1 (https=)

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WO2020150239A1 (en) * 2019-01-14 2020-07-23 University Of Washington Apparatuses, systems and methods for solid immersion meniscus lenses
DE102019207873B4 (de) * 2019-05-29 2021-10-14 Leica Microsystems Cms Gmbh Optischer Aufbau für ein Mikroskop und Mikroskop
WO2021041807A1 (en) * 2019-08-30 2021-03-04 The Regents Of The University Of California Systems and methods for image cytometry
DE102020200193A1 (de) * 2020-01-09 2021-07-15 Albert-Ludwigs-Universität Freiburg Anordnung zur optischen Vorkonditionierung einer optisch aktivierbaren biologischen Probe
GB202001372D0 (en) * 2020-01-31 2020-03-18 Res & Innovation Uk Bioprinters
GB2595520A (en) * 2020-05-29 2021-12-01 Oxford Nanoimaging Ltd A diagnostic device and method
CN116324622A (zh) * 2020-08-11 2023-06-23 加泰罗尼亚生物工程基础研究所 用于立体光刻三维打印的设备和方法
CN112834410B (zh) * 2021-01-04 2022-09-13 桂林电子科技大学 基于双芯光纤光操控的片状光显微成像方法及装置
DE102021104871A1 (de) 2021-03-01 2022-09-01 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zur lichtblattmikroskopischen Untersuchung einer Probe
EP4067866B1 (en) * 2021-04-01 2025-11-05 Leica Microsystems CMS GmbH Imaging system and method for imaging biological samples
WO2022268325A1 (en) 2021-06-24 2022-12-29 Leica Microsystems Cms Gmbh Calibration object for calibrating an imaging system
WO2023057349A1 (en) * 2021-10-05 2023-04-13 Leica Microsystems Cms Gmbh Imaging system and method
US20240410818A1 (en) 2021-10-05 2024-12-12 Leica Microsystems Cms Gmbh Sample carrier and method for imaging a sample
EP4594808A1 (en) * 2022-09-26 2025-08-06 Consiglio Nazionale Delle Ricerche System and method for multicolor light sheet microscopy
CN116841026B (zh) * 2023-06-13 2026-03-24 武汉精立电子技术有限公司 一种显微成像方法、设备及存储介质
DE102024205127A1 (de) * 2024-06-04 2025-12-04 Carl Zeiss Microscopy Gmbh Bildgebungsverfahren für lichtdurchlässige Proben, optische Anordnung und Mikroskop
FR3166207A1 (fr) * 2024-09-10 2026-03-13 Universite De Bourgogne Olfactomètre d’étude comportementale de spécimen animal et procédé d’étude comportementale associé
US20260091385A1 (en) * 2024-09-30 2026-04-02 Illumina, Inc. Apparatus and methods for sample loading

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DE10257423A1 (de) 2002-12-09 2004-06-24 Europäisches Laboratorium für Molekularbiologie (EMBL) Mikroskop
DE102005028893B4 (de) * 2005-06-19 2007-12-06 Stiftung Alfred-Wegener-Institut für Polar- und Meeresforschung Stiftung des öffentlichen Rechts Vorrichtung zur Partikeldetektion in einer tiefenbegrenzten Lichtscheibe
DE102007048409A1 (de) 2007-10-09 2009-04-16 Carl Zeiss Microimaging Gmbh Verfahren zum Positionieren von biologischen Proben in einer mikroskopischen Anordnung
US10051240B2 (en) 2010-06-14 2018-08-14 Howard Hughes Medical Institute Structured plane illumination microscopy
KR20120084681A (ko) * 2011-01-20 2012-07-30 한양대학교 산학협력단 유세포 분석을 이용한 나노 물질 위해성 평가 방법
US9074980B2 (en) * 2011-01-20 2015-07-07 Industry-University Corporation Foundation Hanyang University Method for the toxicity assessments of nano-materials
US10908403B2 (en) 2011-02-14 2021-02-02 European Molecular Biology Laboratory (Embl) Light-pad microscope for high-resolution 3D fluorescence imaging and 2D fluctuation spectroscopy
US9316824B2 (en) 2011-03-04 2016-04-19 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Optomechanical module for converting a microscope to provide selective plane illumination microscopy
DE102012110077A1 (de) 2012-10-23 2014-06-26 Karlsruher Institut für Technologie Mikroskop mit mindestens einem Beleuchtungsstrahl in Form einer Lichtscheibe
DE102013107297B4 (de) 2013-07-10 2025-04-24 Carl Zeiss Microscopy Gmbh Anordnung zur Lichtblattmikroskopie
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DE102013112596B4 (de) * 2013-11-15 2023-12-28 Carl Zeiss Microscopy Gmbh Anordnung zur Lichtblattmikroskopie
US20160097028A1 (en) * 2014-10-03 2016-04-07 Academia Sinica Microfluidic device for cell spheroid culture and analysis
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ITUB20153920A1 (it) * 2015-09-28 2017-03-28 Milano Politecnico Dispositivo optofluidico.
GB201602557D0 (en) * 2016-02-12 2016-03-30 Imp Innovations Ltd Specimen mounting device and method for live microscopy
DE102016204653B4 (de) * 2016-03-21 2022-12-22 Carl Zeiss Microscopy Gmbh Die Erfindung betrifft ein Lichtblattmikroskop sowie ein Verfahren zum Betreiben eines Lichtblattmikroskops

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Publication number Publication date
CN111164485A (zh) 2020-05-15
JP2020535421A (ja) 2020-12-03
DE102017122718A1 (de) 2019-04-04
WO2019063539A1 (de) 2019-04-04
JP7309698B2 (ja) 2023-07-18
US20200284715A1 (en) 2020-09-10
US11371927B2 (en) 2022-06-28

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