CN111157499A - Method for calibrating fluorescence detection instrument - Google Patents
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Abstract
The invention provides a method for calibrating a fluorescence detection instrument, which adopts the existing standard equipment determined by measurement to carry out calibration operation, ensures standard traceability and certainty by the calibration operation, can be suitable for large-batch and accurate measurement and improves the quality consistency of the fluorescence detection instrument in the batch production process, and can also be used for carrying out quality control on the fluorescence detection instrument and ensuring the consistency of performance parameters of the fluorescence detection instrument.
Description
Technical Field
The invention relates to the technical field of biological fluorescence detection, in particular to a method for calibrating a fluorescence detection instrument.
Background
The fluorescence detector mainly utilizes the fluorescence characteristic that a fluorescent substance enters an excited state after receiving incident light with a certain wavelength, and immediately emits emergent light with a wavelength different from that of the incident light after absorbing light energy, the fluorescent substance is correspondingly excited to form emitted light with a wavelength of lambda 2 by using light with the wavelength of lambda 1 as excitation light and irradiating the excitation light on the fluorescent substance, and then the content of a substance marked by the fluorescent substance can be indirectly obtained by detecting the intensity of the emitted light with the wavelength of lambda 2, and the operation process of the fluorescence detector in the prior art is shown in fig. 1. At present, the fluorescence detector is calibrated by using a fixed content of fluorescent substance as a default standard and placing the fluorescent substance on the fluorescence detector for measurement. If there are several instruments to be calibrated, the calibration is carried out by using the substance with fixed fluorescence content as standard. This has a problem that the fluorescence intensity of the fluorescent substance of a fixed content cannot be fixed. Since the fluorescence property is such that the fluorescent substance decays after irradiation or after a long time of standing, the emitted light is weakened, which is problematic if it is used to calibrate multiple instruments, since the fluorescence intensity of the standard substance has changed, which causes uncertainty in the calibration process of the fluorescence detection instrument, which affects the normal operation of the fluorescence detection instrument.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a method for calibrating a fluorescence detection instrument, which comprises the following steps: step S1, performing first calibration processing on the luminous intensity of an excitation light source corresponding to the fluorescence detector through a light intensity measuring instrument placed at the original test strip position of the fluorescence detector, wherein the luminous wavelength of the excitation light source is lambda 1; step S2, performing a second calibration process on the detection precision of the signal detector corresponding to the fluorescence detector by using a standard light source placed at the original test strip position of the fluorescence detector, wherein the standard light source has a light-emitting wavelength of λ 2, and λ 1 is different from λ 2; therefore, the calibration method of the fluorescence detection instrument adopts the existing standard equipment determined by measurement to carry out calibration operation, the calibration operation ensures standard traceability and certainty, the method can be suitable for large-batch and accurate measurement and improves the quality consistency of the fluorescence detection instrument in the batch production process, and in addition, the method can also be used for carrying out quality control on the fluorescence detection instrument and ensuring the consistency of performance parameters of the fluorescence detection instrument.
The invention provides a method for calibrating a fluorescence detection instrument, which is characterized by comprising the following steps:
step S1, performing first calibration processing on the luminous intensity of an excitation light source corresponding to the fluorescence detector through a light intensity measuring instrument placed at the original test strip position of the fluorescence detector, wherein the luminous wavelength of the excitation light source is lambda 1;
step S2, performing second calibration processing on the detection precision of a signal detector corresponding to the fluorescence detection instrument through a standard light source placed at the original test strip position of the fluorescence detection instrument, wherein the standard light source has a light-emitting wavelength of lambda 2, and lambda 1 is different from lambda 2;
further, in step S1, the performing, by the light intensity measuring instrument placed at the original test strip position of the fluorescence detector, a first calibration process on the emission intensity of the excitation light source corresponding to the fluorescence detector specifically includes,
step S101, acquiring first attribute information of the original test strip position of the fluorescence detector, and performing first layout operation on the light intensity measuring instrument according to the first attribute information;
step S102, acquiring second attribute information about the position of the excitation light source according to the result of the first layout operation;
step S103, adjusting the light-emitting working mode of the excitation light source device according to the second attribute information so as to realize the first calibration processing about the light-emitting intensity;
further, in the step S101, acquiring first attribute information about an original test strip position of the fluorescence detector, and performing a first layout operation on the light intensity measuring instrument according to the first attribute information specifically includes,
step S1011, acquiring position area data and/or position orientation data of an original test strip position of the fluorescence detection instrument as the first attribute information;
step S1012, acquiring light receiving area data and light receiving sensitivity data about the light intensity measuring instrument;
step S1013, determining the optimal layout position of the light intensity measuring instrument according to the first attribute information, the light receiving area data and the light receiving sensitivity data,
wherein the optimal deployment location is determined by:
assuming that the area of a position region of an original test strip position is S, the orientation angle of the position region is j, the area of a light receiving region of the light intensity measuring instrument is St, the light receiving sensitivity is M, the detection time is t, and the environment coefficient is H, wherein the environment coefficient is determined by the environment temperature, the environment humidity and the environment gas component, and is calculated by the following formula (1)
In the above formula (1), P is the ambient temperature, Q is the ambient humidity, and zi、FiThe refractive index and the concentration of a gas i are respectively shown, i is the number of the environmental gas component, and i is 1, 2, 3, … and n;
the optimal layout position P (x, y) is calculated by the following formula (2)
In the formula (2), K (x, y) is a position coordinate of the original test strip relative to the light source;
step S1014, according to the optimal layout position, performing the first layout operation on the light intensity measuring instrument;
further, in the step S102, acquiring second attribute information on the position of the excitation light source according to the result of the first arrangement operation specifically includes,
step S1021, acquiring size information and light emitting port position information of the excitation light source device as a part of the second attribute information;
step S1022, determining, as part of the second attribute information, relative pose information between the light intensity measuring instrument and the excitation light source device according to the result of the first arrangement operation;
further, in the step S103, adjusting the light emitting operation mode of the excitation light source device according to the second attribute information to realize the first calibration process on the light emitting intensity specifically includes,
a step S1031 of extracting size information and light emission port position information about the excitation light source device and relative pose information between the light intensity measuring instrument and the excitation light source device from the second attribute information;
step S1032, constructing a light emission model of the excitation light source device according to the size information, the light emission port position information and the relative pose information;
step S1033, performing optimization processing on the light emission model to adjust a light emission operation mode of the excitation light source device, thereby implementing the first calibration processing regarding the light emission intensity;
further, in the step S1033, the optimizing the light emission model to adjust the light emitting operation mode of the excitation light source device, so as to realize the first calibration process regarding the light emitting intensity specifically includes,
step S10331 of performing optimization processing on the light emission model with respect to light path properties and/or circuit properties of an excitation light source to determine light path parameters and/or circuit parameters to be adjusted with respect to the excitation light source;
step S10332, adjusting an optical focusing parameter and/or a power supply circuit parameter of the excitation light source device according to the optical path parameter and/or the circuit parameter to be adjusted, so as to implement the first calibration process;
further, in the step S2, the performing a second calibration process on the signal detector corresponding to the fluorescence detection apparatus with respect to the detection accuracy by using the standard light source disposed at the position of the test strip existing in the fluorescence detection apparatus specifically includes,
step S201, acquiring first attribute information of an original test strip position of the fluorescence detector and imaging attribute information of an optical module of the fluorescence detector;
step S202, performing second layout operation on the standard light source according to the first attribute information and the imaging attribute information;
step S203, acquiring third attribute information about the signal detector according to the result of the second layout operation;
step S204, according to the third attribute information, adjusting the detection working mode of the signal detector to realize the second calibration processing about the detection precision;
further, in step S201, the acquiring of the first attribute information about the original strip position of the fluorescence detection instrument and the imaging attribute information of the optical module of the fluorescence detection instrument specifically includes,
acquiring position area data and/or position orientation data of an original test strip position of the fluorescent detection instrument as the first attribute information, and acquiring at least one of an exposure parameter, a focusing parameter and a filtering parameter of the imaging module as the imaging attribute information;
alternatively, the first and second electrodes may be,
in the step S202, performing a second layout operation on the standard light source according to the first attribute information and the imaging attribute information specifically includes,
step S2021, determining the optimal layout position of the standard light source according to the first attribute information and the imaging attribute information;
step S2022, performing the second layout operation on the standard light source according to the optimal layout position;
further, in the step S203, acquiring third attribute information on the signal detector according to the result of the second layout operation specifically includes,
acquiring the light receiving area size information and the photoelectric conversion sensitivity information of the signal detector to obtain the third attribute information;
further, in the step S204, adjusting the detection operation mode of the signal detector according to the third attribute information to realize the second calibration process regarding the detection accuracy specifically includes,
step S2041 of extracting information on the size of the light receiving area and information on the photoelectric conversion sensitivity of the signal detector from the third attribute information, thereby relating to a light reception sensing model of the signal detector;
step S2042, performing optimization processing on the light receiving sensing model to adjust a photoelectric conversion operating mode of the signal detector, so that the minimum signal detection intensity and/or detection sensitivity of the signal detector satisfy a preset condition, thereby implementing the second calibration processing.
Compared with the prior art, the calibration method of the fluorescence detection instrument adopts the existing standard equipment determined by measurement to carry out calibration operation, the calibration operation ensures standard traceability and certainty, the method can be suitable for large-batch and accurate measurement and improves the quality consistency of the fluorescence detection instrument in the batch production process, and in addition, the method can also be used for carrying out quality control on the fluorescence detection instrument and ensuring the consistency of performance parameters of the fluorescence detection instrument.
Additional features and advantages of the invention will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. The objectives and other advantages of the invention will be realized and attained by the structure particularly pointed out in the written description and claims hereof as well as the appended drawings.
The technical solution of the present invention is further described in detail by the accompanying drawings and embodiments.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
Fig. 1 is an operation diagram of a calibration method of a fluorescence detector provided in the prior art.
FIG. 2 is a schematic flow chart of a method for calibrating a fluorescence detection instrument according to the present invention.
FIG. 3 is a schematic diagram illustrating the operation of step S1 in the calibration method of the fluorescence detection instrument according to the present invention.
FIG. 4 is a schematic diagram illustrating the operation of step S2 in the calibration method of the fluorescence detection instrument according to the present invention.
Reference numerals: 1. an excitation light source; 2. a test strip; 3. a signal detector; 4. an optical module; 5. a light intensity detector; 6. a standard light source.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Fig. 2 is a schematic flow chart of a calibration method of a fluorescence detection instrument according to an embodiment of the present invention. The calibration method of the fluorescence detection instrument comprises the following steps:
step S1, performing first calibration processing on the luminous intensity of an excitation light source corresponding to the fluorescence detector through a light intensity measuring instrument placed at the original test strip position of the fluorescence detector, wherein the luminous wavelength of the excitation light source is lambda 1;
and step S2, performing second calibration processing on the detection precision of the signal detector corresponding to the fluorescence detector through a standard light source placed at the original test strip position of the fluorescence detector, wherein the standard light source has a light-emitting wavelength of λ 2, and λ 1 is different from λ 2.
Preferably, in step S1, the performing a first calibration process on the excitation light source corresponding to the fluorescence detector by the light intensity measuring instrument placed at the original test strip position of the fluorescence detector specifically includes,
step S101, acquiring first attribute information of the original test strip position of the fluorescence detector, and performing first layout operation on the light intensity measuring instrument according to the first attribute information;
step S102, acquiring second attribute information about the position of the excitation light source according to the result of the first layout operation;
step S103, adjusting a light emitting operation mode of the excitation light source according to the second attribute information to implement the first calibration process regarding the light emitting intensity.
Preferably, in step S101, obtaining first attribute information about the original position of the fluorescence detector, and performing a first layout operation on the light intensity measuring instrument according to the first attribute information specifically includes,
step S1011, obtaining position area data and/or position orientation data of the original test strip position of the fluorescence detector as the first attribute information;
step S1012, acquiring light receiving area data and light receiving sensitivity data about the light intensity measuring instrument;
step S1013, determining the optimal layout position of the light intensity measuring instrument according to the first attribute information, the light receiving area data and the light receiving sensitivity data,
wherein the optimal layout position is determined by the following process:
assuming that the area of the position region of the original test strip position is S, the orientation angle of the position region is j, the area of the light receiving region of the light intensity measuring instrument is St, the light receiving sensitivity is M, the detection time is t, and the environment coefficient is H, the environment coefficient is determined by the environment temperature, the environment humidity and the environment gas component, and the environment coefficient H is calculated by the following formula (1)
In the above formula (1), P is the ambient temperature, Q is the ambient humidity, and zi、FiThe refractive index and the concentration of a gas i are respectively shown, i is the number of the environmental gas component, and i is 1, 2, 3, … and n;
the optimal layout position P (x, y) is calculated by the following formula (2)
In the formula (2), K (x, y) is a position coordinate of the original test strip relative to the light source;
step S1014, according to the optimal layout position, performing the first layout operation on the light intensity measuring instrument;
the method for determining the optimal layout position not only integrates the factors such as the position area, the orientation angle, the light receiving area, the light receiving sensitivity and the like, but also takes the environmental factors such as the temperature, the humidity, the air composition and the like into consideration, so that the interference of the environment on the determination of the optimal layout position can be eliminated, and the determination accuracy of the optimal layout position is improved.
Preferably, in the step S102, acquiring the second attribute information on the position of the excitation light source according to the result of the first layout operation specifically includes,
step S1021, acquiring size information and light emitting port position information of the excitation light source as a part of the second attribute information;
step S1022, determining the relative pose information between the light intensity measuring instrument and the excitation light source device as a part of the second attribute information according to the result of the first layout operation.
Preferably, in the step S103, adjusting the light emitting operation mode of the excitation light source according to the second attribute information to realize the first calibration process on the light emitting intensity specifically includes,
step S1031 of extracting size information and light emission port position information about the excitation light source device and relative pose information between the light intensity measuring instrument and the excitation light source device from the second attribute information;
step S1032 of constructing a light emission model concerning the excitation light source device based on the size information, the light emission port position information, and the relative pose information;
step S1033, performing an optimization process on the light emission model to adjust a light emission operation mode of the excitation light source, thereby implementing the first calibration process regarding the light emission intensity.
Preferably, in step S1033, the optimizing process of the light emission model to adjust the light emitting operation mode of the excitation light source device, so as to realize the first calibration process regarding the light emitting intensity specifically includes,
step S10331 of performing optimization processing on the light emission model with respect to light path properties and/or circuit properties of the excitation light source to determine light path parameters and/or circuit parameters to be adjusted with respect to the excitation light source;
step S10332, adjusting an optical focusing parameter and/or a power supply circuit parameter of the excitation light source device according to the optical path parameter and/or the circuit parameter to be adjusted, so as to implement the first calibration process.
Preferably, in the step S2, the performing a second calibration process on the signal detector corresponding to the fluorescence detector by using the standard light source disposed at the original test strip position of the fluorescence detector specifically includes,
step S201, acquiring first attribute information of an original test strip position of the fluorescence detector and imaging attribute information of an optical module of the fluorescence detector, wherein the optical module can be at least one of a lens, a lens group and a filter and any number of combinations thereof;
step S202, performing second layout operation on the standard light source according to the first attribute information and the imaging attribute information;
step S203, acquiring third attribute information about the signal detector according to the result of the second layout operation;
step S204, according to the third attribute information, adjusting the detection operation mode of the signal detector to realize the second calibration process about the detection precision.
Preferably, in step S201, the acquiring of the first attribute information about the original strip position of the fluorescence detection instrument and the imaging attribute information of the optical module of the fluorescence detection instrument specifically includes,
acquiring position area data and/or position orientation data of an original test strip position of the fluorescence detector as the first attribute information, and acquiring at least one of exposure parameters, focusing parameters and filtering parameters of the imaging module as the imaging attribute information.
Preferably, in the step S202, performing the second layout operation on the standard light source according to the first attribute information and the imaging attribute information specifically includes,
step S2021, determining the optimal layout position of the standard light source according to the first attribute information and the imaging attribute information;
step S2022, performing the second layout operation on the standard light sources according to the optimal layout position.
Preferably, in the step S203, acquiring the third attribute information on the signal detector according to the result of the second laying operation specifically includes,
the light receiving area size information and the photoelectric conversion sensitivity information of the signal detector are acquired as the third attribute information.
Preferably, in the step S204, adjusting the detection operation mode of the signal detector according to the third attribute information to realize the second calibration process regarding the detection accuracy specifically includes,
step S2041, extracting information on the size of the light receiving area and information on the photoelectric conversion sensitivity of the signal detector from the third attribute information, thereby relating to the light reception sensing model of the signal detector;
step S2042, performing optimization processing on the light receiving sensing model to adjust the photoelectric conversion operating mode of the signal detector, so that the minimum signal detection intensity and/or detection sensitivity of the signal detector satisfy a preset condition, thereby implementing the second calibration processing.
Referring to fig. 3 and 4, there are shown schematic diagrams of the operation of step S1 and step S2 in the calibration method of the fluorescence detection instrument according to the present invention. The contents illustrated in fig. 3 and 4 are the same as those described above with respect to step S1 and step S2, and the same description will not be repeated here.
From the above description, it can be seen that the calibration method of the fluorescence detection apparatus uses the existing standard equipment determined by measurement to perform calibration operation, and the calibration operation ensures standard traceability and certainty, which can be suitable for mass and precise measurement and improve quality consistency of the fluorescence detection apparatus in mass production process.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.
Claims (10)
1. A method for calibrating a fluorescence detection instrument, comprising the steps of:
step S1, performing first calibration processing on the luminous intensity of an excitation light source corresponding to the fluorescence detector through a light intensity measuring instrument placed at the original test strip position of the fluorescence detector, wherein the luminous wavelength of the excitation light source is lambda 1;
and step S2, performing second calibration processing on the detection precision of the signal detector corresponding to the fluorescence detection instrument through a standard light source placed at the original test strip position of the fluorescence detection instrument, wherein the standard light source has a light-emitting wavelength of lambda 2, and lambda 1 is different from lambda 2.
2. The method for calibrating a fluorescence detection instrument according to claim 1, wherein:
in step S1, the performing, by the light intensity measuring instrument placed at the original test strip position of the fluorescence detector, a first calibration process on the emission intensity of the excitation light source corresponding to the fluorescence detector specifically includes,
step S101, acquiring first attribute information of the original test strip position of the fluorescence detector, and performing first layout operation on the light intensity measuring instrument according to the first attribute information;
step S102, acquiring second attribute information about the position of the excitation light source according to the result of the first layout operation;
step S103, adjusting a light emitting operation mode of the excitation light source according to the second attribute information, so as to implement the first calibration process about the light emitting intensity.
3. The method of calibrating a fluorescence detector according to claim 2, wherein:
in step S101, acquiring first attribute information about an original test strip position of the fluorescence detector, and performing a first layout operation on the light intensity measuring instrument according to the first attribute information specifically includes,
step S1011, acquiring position area data and/or position orientation data of an original test strip position of the fluorescence detection instrument as the first attribute information;
step S1012, acquiring light receiving area data and light receiving sensitivity data about the light intensity measuring instrument;
step S1013, determining the optimal layout position of the light intensity measuring instrument according to the first attribute information, the light receiving area data and the light receiving sensitivity data,
wherein the optimal deployment location is determined by:
assuming that the area of a position region of an original test strip position is S, the orientation angle of the position region is j, the area of a light receiving region of the light intensity measuring instrument is St, the light receiving sensitivity is M, the detection time is t, and the environment coefficient is H, wherein the environment coefficient is determined by the environment temperature, the environment humidity and the environment gas component, and is calculated by the following formula (1)
In the above formula (1), P is the ambient temperature, Q is the ambient humidity, and zi、FiThe refractive index and the concentration of a gas i are respectively shown, i is the number of the environmental gas component, and i is 1, 2, 3, … and n;
the optimal layout position P (x, y) is calculated by the following formula (2)
In the formula (2), K (x, y) is a position coordinate of the original test strip relative to the light source;
and step S1014, performing the first layout operation on the light intensity measuring instrument according to the optimal layout position.
4. The method of calibrating a fluorescence detector according to claim 2, wherein:
in the step S102, acquiring second attribute information on the position of the excitation light source according to the result of the first layout operation specifically includes,
step S1021, acquiring size information and light emitting port position information of the excitation light source device as a part of the second attribute information;
step S1022, determining the relative pose information between the light intensity measuring instrument and the excitation light source device as a part of the second attribute information according to the result of the first arrangement operation.
5. The method of calibrating a fluorescence detector according to claim 2, wherein:
in step S103, adjusting the light emitting operation mode of the excitation light source according to the second attribute information to implement the first calibration process on the light emitting intensity specifically includes,
a step S1031 of extracting size information and light emission port position information about the excitation light source device and relative pose information between the light intensity measuring instrument and the excitation light source device from the second attribute information;
step S1032, constructing a light emission model of the excitation light source device according to the size information, the light emission port position information and the relative pose information;
step S1033, performing optimization processing on the light emission model to adjust a light emission operation mode of the excitation light source device, thereby implementing the first calibration processing regarding the light emission intensity.
6. The method for calibrating a fluorescence detection instrument according to claim 5, wherein:
in step S1033, the optimizing the light emission model to adjust the light emitting operation mode of the excitation light source device so as to realize the first calibration process regarding the light emitting intensity specifically includes,
step S10331 of performing optimization processing on the light emission model with respect to light path properties and/or circuit properties of an excitation light source to determine light path parameters and/or circuit parameters to be adjusted with respect to the excitation light source;
step S10332, adjusting an optical focusing parameter and/or a power supply circuit parameter of the excitation light source device according to the optical path parameter and/or the circuit parameter to be adjusted, so as to implement the first calibration process.
7. The method for calibrating a fluorescence detection instrument according to claim 1, wherein:
in step S2, the performing a second calibration process on the signal detector corresponding to the fluorescence detection apparatus with respect to the detection accuracy by the standard light source disposed at the position of the test strip existing in the fluorescence detection apparatus specifically includes,
step S201, acquiring first attribute information of an original test strip position of the fluorescence detector and imaging attribute information of an optical module of the fluorescence detector;
step S202, performing second layout operation on the standard light source according to the first attribute information and the imaging attribute information;
step S203, acquiring third attribute information about the signal detector according to the result of the second layout operation;
step S204, adjusting the detection operation mode of the signal detector according to the third attribute information, so as to implement the second calibration processing regarding the detection precision.
8. The method for calibrating a fluorescence detection instrument according to claim 7, wherein:
in step S201, acquiring first attribute information about an original test strip position of the fluorescence detection apparatus and imaging attribute information of an optical module of the fluorescence detection apparatus specifically includes acquiring position area data and/or position orientation data about the original test strip position of the fluorescence detection apparatus as the first attribute information, and acquiring at least one of an exposure parameter, a focusing parameter, and a filtering parameter about the imaging module as the imaging attribute information;
alternatively, the first and second electrodes may be,
in the step S202, performing a second layout operation on the standard light source according to the first attribute information and the imaging attribute information specifically includes,
step S2021, determining the optimal layout position of the standard light source according to the first attribute information and the imaging attribute information;
step S2022, performing the second layout operation on the standard light sources according to the optimal layout position.
9. The method for calibrating a fluorescence detection instrument according to claim 7, wherein:
in the step S203, acquiring third attribute information on the signal detector according to the result of the second layout operation specifically includes,
and acquiring the light receiving area size information and the photoelectric conversion sensitivity information of the signal detector to obtain the third attribute information.
10. The method for calibrating a fluorescence detection instrument according to claim 7, wherein:
in the step S204, adjusting the detection operation mode of the signal detector according to the third attribute information to realize the second calibration process regarding the detection accuracy specifically includes,
step S2041 of extracting information on the size of the light receiving area and information on the photoelectric conversion sensitivity of the signal detector from the third attribute information, thereby relating to a light reception sensing model of the signal detector;
step S2042, performing optimization processing on the light receiving sensing model to adjust a photoelectric conversion operating mode of the signal detector, so that the minimum signal detection intensity and/or detection sensitivity of the signal detector satisfy a preset condition, thereby implementing the second calibration processing.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104483297A (en) * | 2014-12-08 | 2015-04-01 | 上海通微分析技术有限公司 | Visualization calibration laser-induced fluorescence detection device |
| CN105928911A (en) * | 2016-04-11 | 2016-09-07 | 深圳市华科瑞科技有限公司 | Calibration method of fluorescence detection instrument |
| CN205898812U (en) * | 2016-04-18 | 2017-01-18 | 深圳市华科瑞科技有限公司 | Automatic adjustment showed auxiliary device of colour during immunity was measured |
| CN205898683U (en) * | 2016-04-11 | 2017-01-18 | 深圳市华科瑞科技有限公司 | Calibration device of fluorescence detector ware |
-
2020
- 2020-01-06 CN CN202010010067.9A patent/CN111157499B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104483297A (en) * | 2014-12-08 | 2015-04-01 | 上海通微分析技术有限公司 | Visualization calibration laser-induced fluorescence detection device |
| CN105928911A (en) * | 2016-04-11 | 2016-09-07 | 深圳市华科瑞科技有限公司 | Calibration method of fluorescence detection instrument |
| CN205898683U (en) * | 2016-04-11 | 2017-01-18 | 深圳市华科瑞科技有限公司 | Calibration device of fluorescence detector ware |
| CN205898812U (en) * | 2016-04-18 | 2017-01-18 | 深圳市华科瑞科技有限公司 | Automatic adjustment showed auxiliary device of colour during immunity was measured |
Non-Patent Citations (2)
| Title |
|---|
| A. SEGRETO ET AL: "Using reference stars for verification of the end-to-end absolute calibration and the long term monitoring of the Fluorescence Detector of the Pierre Auger Observatory", 《NUCLEAR AND PARTICLE PHYSICS PROCEEDINGS》 * |
| 李月玥: "ATP 荧光检测仪校准方法研究", 《质量探索》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113030501A (en) * | 2021-03-05 | 2021-06-25 | 郑州如飞生物技术有限公司 | Automatic calibration mechanism for fluorescence immunoassay analyzer and automatic calibration method thereof |
| CN113030501B (en) * | 2021-03-05 | 2023-07-21 | 郑州如飞生物技术有限公司 | Automatic calibration mechanism for fluorescence immunoassay analyzer and automatic calibration method thereof |
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