CN205898683U - Calibration device of fluorescence detector ware - Google Patents
Calibration device of fluorescence detector ware Download PDFInfo
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- CN205898683U CN205898683U CN201620301231.0U CN201620301231U CN205898683U CN 205898683 U CN205898683 U CN 205898683U CN 201620301231 U CN201620301231 U CN 201620301231U CN 205898683 U CN205898683 U CN 205898683U
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Abstract
The utility model discloses a calibration device of fluorescence detector ware for calibration when the fluorescence detector tolerance is produced, including at least one fluorescence detector ware, still including a luminous intensity measurement appearance and a CIE standard light source, the fluorescence detector ware is including detecting the station, luminous intensity measurement appearance and CIE standard light source's structure all with the station adaptation is detected, the uniformity of fluorescence detector ware in the volume production can be improved in using.
Description
Technical field
The utility model is related to the fluorescence detection equipment in biologic medical field, more particularly, to a kind of calibrator (-ter) unit of fluorescence detection equipment.
Background technology
Fluorescent material characteristic is: when fluorescent material through certain wavelength incident light irradiate, absorb luminous energy after enter excitation state, and immediately de excitation send out and send different from incident light wavelength emergent light；And once stopping incident light, luminescence phenomenon also disappears therewith immediately.The emergent light with this property is just referred to as fluorescence.
As shown in Figure 1, fluorescence detection equipment (luminoscope) includes light source 1, optical filter 3, signal detection module 4 and microscope group, its operation principle is by irradiating on the fluorescent substance with the light (referred to as exciting light) of the first wavelength, fluorescent material launches the light (referred to as launching light) of second wavelength, by detecting the light intensity of second wavelength, indirectly obtain the content of the material of fluorescent material mark.
At present, the calibration of luminoscope is as default standard with constant concentration fluorescent material, is put into measurement on fluorescent instrument.To calibrate if there are multiple stage instrument and just be calibrated as standard with this fixing fluorescence content material.The problem of do so is that the fluorescence intensity of constant concentration fluorescent material can not be fixed.Because fluorescent material can decay after placing after fluorescent characteristic is irradiation or for a long time, the light launched will weaken, if will go wrong, because standard substance fluorescence intensity has changed and for for calibrating multiple stage instrument.Or the instrument of calibration today, will be calibrated with same fluorescence, value all can change tomorrow again.
This scheme cannot ensure the uniformity of machine when big measuring appratus produce, and can only ensure one probably.
Content of the invention
The main technical problems to be solved in the utility model is to provide a kind of calibrator (-ter) unit of fluorescence detection equipment, and it can improve the uniformity of fluorescence detection equipment in volume production.
For solving above-mentioned technical problem, a kind of calibrator (-ter) unit of fluorescence detection equipment, include at least one fluorescence detection equipment, also include a light intensity measuring instrument and a standard sources, described fluorescence detection equipment includes detection station, and the structure of described light intensity measuring instrument and standard sources is all adapted to described detection station.
Described light intensity measuring instrument includes substrate, is provided with sensor devices, light intensity indicating circuit and power supply on substrate, and three's circuit couples；When sensor devices receive the irradiation of light, convert optical signals to electric signal, input light intensity indicating circuit, light intensity indicating circuit enters prompting state when light signal strength reaches preset value；Power supply is powered for light intensity indicating circuit.
Described light intensity indicating circuit enters prompting state and refers to: light intensity indicating circuit includes numerical value display screen, indicator lamp or buzzer, to should device energising.
Described standard sources includes substrate, is provided with luminescent device, drive circuit and power supply on substrate, and three's circuit couples, and power supply is drive circuitry, and drive circuit drives luminescent device to light.
Described luminescent device is LED light source.
It is arranged with a housing, the setting windowing of housing corresponding luminescent device position is so that luminescent device exposes outside described standard sources.
The beneficial effects of the utility model are: a kind of calibrator (-ter) unit of fluorescence detection equipment, include at least one fluorescence detection equipment, also include a light intensity measuring instrument and a standard sources, described fluorescence detection equipment includes detection station, the structure of described light intensity measuring instrument and standard sources is all adapted to described detection station, can improve the uniformity of fluorescence detection equipment in volume production in application.
Brief description
Fig. 1 is calibration (test) schematic diagram of existing fluorescence detection equipment；
Fig. 2 is a kind of schematic diagram of the detecting step one of embodiment of the utility model；
Fig. 3 is a kind of schematic diagram of the detecting step two of embodiment of the utility model；
Fig. 4 is the utility model standard sources electrical block diagram；
Fig. 5 is the utility model light intensity measuring instrument electrical block diagram.
Specific embodiment
As shown in figure 1, being a kind of calibrating principle (light path) figure of fluorescence detection equipment instrument 5.There is fluorescent material in test strips 2 in actual test, be irradiated in test strips 2 with the light source 1 of l1, excite the fluorescence in test strips 2, by the filtration of optical filter 3, the fluorescence of l2 can only be allowed to enter into signal detection module 4.Prior art is with being as default standard (calibration) with constant concentration fluorescent material, is put into measurement on fluorescent instrument 5.The fluorescence that fluorescent material sends can be decayed (fluorescent material can the change of change over time or illumination and change), has uncertainty with fluorescent material as reference material, difference after the meeting on calibration machine performance out.Certainly, also have the luminoscope light path of other species in prior art, but essence be all with l1(wavelength 1) light source activation, detect l2(wavelength 2) light intensity, no longer enumerate herein.
A kind of calibrator (-ter) unit of fluorescence detection equipment of the utility model, include at least one fluorescence detection equipment, also include a light intensity measuring instrument 6 and a standard sources 7, described fluorescence detection equipment includes detection station (i.e. test strips 2 position shown in Fig. 1), and the structure of described light intensity measuring instrument 6 and standard sources 7 is all adapted to described detection station.
As shown in figure 4, standard sources 7 includes substrate, luminescent device 9, drive circuit 10 and power supply 8 are provided with substrate, three's circuit couples, power supply 8 is powered for drive circuit 10, drive circuit 10 drives luminescent device 9 to light.
During enforcement, luminescent device 9 can be LED light source.
During enforcement, the outside sheathed housing of standard sources 7, the setting windowing of corresponding luminescent device 9 position, so that luminescent device 9 exposes, forms test card pattern.
As shown in Figure 5, light intensity measuring instrument 6 includes substrate, sensor devices 12, light intensity indicating circuit 13 and power supply 11 are provided with substrate, three's circuit couples, sensor devices 12 receive light source 1 and irradiate, and convert optical signals to electric signal, input light intensity indicating circuit 13, light intensity indicating circuit 13 enters prompting state when light signal strength reaches preset value.Power supply 11 is powered for light intensity indicating circuit 13.
Described light intensity indicating circuit 13 enters prompting state and refers to: light intensity indicating circuit 13 includes numerical value display screen, indicator lamp or buzzer, to should device energising.
Described fluorescence detection equipment (luminoscope) includes light source 1, optical filter 3, signal detection module 4 and microscope group.
In light source 1 intensity detection of fluorescence detection equipment, and signal detection module 4 parametric calibration is independently carried out in two steps, and light source 1 intensity detection uses light intensity measuring instrument 6 to measure luminous intensity, and signal detection module 4 parametric calibration is used standard sources 7 as detection light source.
Shown in reference picture 2-5, the calibrator (-ter) unit of the utility model fluorescence detection equipment, in calibration, by measuring light source 1 with light intensity measuring instrument 6, with the mode with standard sources 7 demarcation signal detection module 4, is realized calibrating.
With reference to shown in Fig. 1,2,3, during the utility model application, the process of calibration luminoscope detecting instrument 5 is:
(1) it is placed on test strips 2(as shown in Figure 1 with miniature light intensity measuring instrument 6) position examines and determine light source 1 luminous intensity.By adjusting light source 1 power supply circuits parameter, light source 1 luminous intensity is adjusted to preset value.The light source 1 of so all luminoscope detecting instruments 5 just can be calibrated to same standard.
(2) use can send the standard sources 7 of the light of wavelength l2 and is placed on test strips 2(as shown in Figure 1) position, turn off the light source 1 of fluorescence detection equipment 5.The parameter of adjustment signal detection module 4, the value that signal detection module 4 is measured is adjusted to preset value.
Adjusted by this two pacings amount, just can be consistent for the adjustment of all fluorescence detection equipment performance parameters.
Key point of the present utility model is can be with existing method to luminoscope Registration.It is unable to the Registration process that Registration process is changed to two pacing amounts what traditional one step surveying was calibrated.Use light intensity measuring instrument scaling light source first, then use standard sources rate-aided signal detection module.With existing standard accurate calibration luminoscope.
The utility model is exactly to adopt method and material that will not in time with illumination variation, realizes the calibration of fluorescence analyser.
The utility model is calibrated using the existing standard device determining through metering, has standard trackability and certainty, can be suitable for high-volume and accurate measurement；The utility model method may further be used to do the Quality Control of instrument simultaneously, and whether detection light source can long operational time decay, if light source decay can change light source.
Above content is of the present utility model to be embodied as being confined to these explanations it is impossible to assert with reference to specific embodiment further detailed description of the utility model.For the utility model person of an ordinary skill in the technical field, without departing from the concept of the premise utility, some simple deduction or replace can also be made, all should be considered as belonging to protection domain of the present utility model.
Claims (6)
1. a kind of calibrator (-ter) unit of fluorescence detection equipment, include at least one fluorescence detection equipment, it is characterized in that: also include a light intensity measuring instrument (6) and a standard sources (7), described fluorescence detection equipment includes detection station, and the structure of described light intensity measuring instrument (6) and standard sources (7) is all adapted to described detection station.
2. the calibrator (-ter) unit of fluorescence detection equipment as claimed in claim 1, it is characterised in that described light intensity measuring instrument (6) includes substrate, is provided with sensor devices (12), light intensity indicating circuit (13) and power supply (11) on substrate, and three's circuit couples；When sensor devices (12) receive the irradiation of light, convert optical signals to electric signal, input light intensity indicating circuit (13), light intensity indicating circuit (13) enters prompting state when light signal strength reaches preset value；Power supply (11) is that light intensity indicating circuit (13) is powered.
3. fluorescence detection equipment as claimed in claim 2 calibrator (-ter) unit it is characterised in that described light intensity indicating circuit (13) enter prompting state refer to: light intensity indicating circuit (13) includes numerical value display screen, indicator lamp or buzzer, to should device energising.
4. the calibrator (-ter) unit of fluorescence detection equipment as claimed in claim 1, it is characterized in that, described standard sources (7) includes substrate, luminescent device (9), drive circuit (10) and power supply (8) are provided with substrate, three's circuit couples, power supply (8) is that drive circuit (10) is powered, and drive circuit (10) drives luminescent device (9) to light.
5. the calibrator (-ter) unit of fluorescence detection equipment as claimed in claim 4 is it is characterised in that described luminescent device (9) is LED light source.
6. it is characterised in that being arranged with a housing outside described standard sources (7), the setting windowing of housing corresponding luminescent device (9) position is so that luminescent device (9) exposes the calibrator (-ter) unit of fluorescence detection equipment as claimed in claim 4.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620301231.0U CN205898683U (en) | 2016-04-11 | 2016-04-11 | Calibration device of fluorescence detector ware |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201620301231.0U CN205898683U (en) | 2016-04-11 | 2016-04-11 | Calibration device of fluorescence detector ware |
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| CN205898683U true CN205898683U (en) | 2017-01-18 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105928911A (en) * | 2016-04-11 | 2016-09-07 | 深圳市华科瑞科技有限公司 | Calibration method of fluorescence detection instrument |
| CN111157499A (en) * | 2020-01-06 | 2020-05-15 | 深圳市华科瑞科技有限公司 | Method for calibrating fluorescence detection instrument |
| CN113933279A (en) * | 2021-11-04 | 2022-01-14 | 珠海丽珠试剂股份有限公司 | Fluorescence analysis assembly detection device and detection method |
-
2016
- 2016-04-11 CN CN201620301231.0U patent/CN205898683U/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105928911A (en) * | 2016-04-11 | 2016-09-07 | 深圳市华科瑞科技有限公司 | Calibration method of fluorescence detection instrument |
| CN111157499A (en) * | 2020-01-06 | 2020-05-15 | 深圳市华科瑞科技有限公司 | Method for calibrating fluorescence detection instrument |
| CN111157499B (en) * | 2020-01-06 | 2021-05-18 | 深圳市华科瑞科技有限公司 | Method for calibrating fluorescence detection instrument |
| CN113933279A (en) * | 2021-11-04 | 2022-01-14 | 珠海丽珠试剂股份有限公司 | Fluorescence analysis assembly detection device and detection method |
| CN113933279B (en) * | 2021-11-04 | 2023-09-15 | 珠海丽珠试剂股份有限公司 | Fluorescence analysis component detection device and detection method |
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