CN205139013U - But fluorescent substance concentration detection device of automatically regulated luminous intensity - Google Patents
But fluorescent substance concentration detection device of automatically regulated luminous intensity Download PDFInfo
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- CN205139013U CN205139013U CN201520808795.9U CN201520808795U CN205139013U CN 205139013 U CN205139013 U CN 205139013U CN 201520808795 U CN201520808795 U CN 201520808795U CN 205139013 U CN205139013 U CN 205139013U
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Abstract
The utility model discloses a but fluorescent substance concentration detection device of automatically regulated luminous intensity, include CPU and the AD converter of being connected with the CPU input, its characterized in that, light source cell, reflection light detecting unit and glimmering light detecting unit are connected respectively to the CPU output, reflection light detecting unit and glimmering light detecting unit output are connected with the AD converter respectively, the light source passes through optical components and introduces reflection light detecting unit and glimmering light detecting unit. Light source luminance is adjusted through the intensity of measuring the reverberation to this device for examine the light intensity that time measuring shines on the solution that awaits measuring at every turn and have stability and uniformity, just so guaranteed the accuracy and the reliability of testing result, and when measuring fluorescence, the adoption that photomultiplier adds controllable integrating circuit has improved the performance detection of unit to faint the fluorescence signal greatly, and the SNR increases, lets this device can measure more weak fluorescence.
Description
Technical field
The utility model relates to medication solution detection technique, particularly relate to a kind of can the fluorescent material concentration detection apparatus of automatically adjust light strength degree.
Background technology
In medical industry, doctor usually need to understand in patient's sample containing certain material number or concentration, and make clinical diagnosis accurately and prescription on this basis.The method of current measurement fluorescence is a lot, but all there is a problem, and when the fluorescence that material sends is comparatively faint (when fluorescent material concentration is lower), it is inaccurate that instrument cannot be tested or test.Its main cause is that exciting light light intensity is not very stable, apparatus measures unit signal to noise ratio (S/N ratio) not high enough caused by.
Summary of the invention
In order to overcome above-mentioned the deficiencies in the prior art, the utility model provide a kind of fast, accurately, can the fluorescent material concentration detection apparatus of automatically adjust light strength degree.
The utility model solves the technical scheme that its technical matters adopts:
A kind of can the fluorescent material concentration detection apparatus of automatically adjust light strength degree, the A/D converter comprising CPU and be connected with CPU input end, it is characterized in that, described CPU output terminal connects light source cell, reflected light detecting unit and fluorescence detection unit respectively, described reflected light detecting unit is connected with A/D converter respectively with fluorescence detection unit output terminal, and described light source is incorporated into reflected light detecting unit and fluorescence detection unit by optical module.
As the improvement of technique scheme, described light source cell comprises the light source regulator and light source that connect successively.
As the improvement of technique scheme, the controlled peak detection circuit that described reflected light detecting unit comprises PIN photodiode and is connected with its output terminal, described controlled peak detection circuit input end is connected with CPU, and output terminal is connected with A/D converter.
As the improvement of technique scheme, described fluorescence detection unit comprises photomultiplier and is connected controlled integrating circuit with photomultiplier output terminal, and described controlled integrating circuit input end is connected with CPU, and output terminal is connected with A/D converter.
As the improvement of technique scheme, described light source adopt adjustable luminance xenon lamp.
The beneficial effects of the utility model have:
The utility model is a kind of can the fluorescent material concentration detection apparatus of automatically adjust light strength degree, light-source brightness is regulated by the intensity measuring reflected light, when making to detect, the light intensity be radiated on solution to be measured has stability and consistance at every turn, this ensures that theres accuracy and the reliability of testing result; And when measuring fluorescence, sensor have employed highly sensitive photomultiplier, also can detect even if fluorescence signal is faint; The signal transacting of controlled integrating circuit, increases the signal to noise ratio (S/N ratio) of unit greatly.Therefore, the employing that photomultiplier adds controlled integrating circuit substantially increases the detection perform of unit to fluorescent signals, and signal to noise ratio (S/N ratio) increases, and allows this device can measure fainter fluorescence.
Accompanying drawing explanation
Below in conjunction with drawings and the specific embodiments, the utility model is described in further detail, wherein:
Fig. 1 is the theory diagram of the utility model embodiment;
Fig. 2 is the light source automatic principle of compensation block diagram of the utility model embodiment;
The fluorescence detection theory diagram of Fig. 3 the utility model embodiment.
Embodiment
See Fig. 1, a kind of can the fluorescent material concentration detection apparatus of automatically adjust light strength degree, the A/D converter comprising CPU and be connected with CPU input end, it is characterized in that, described CPU output terminal connects light source cell, reflected light detecting unit and fluorescence detection unit respectively, described reflected light detecting unit is connected with A/D converter respectively with fluorescence detection unit output terminal, and described light source is incorporated into reflected light detecting unit and fluorescence detection unit by optical module.
With further reference to Fig. 2, light source cell comprises the light source regulator and light source that connect successively.When electrifying startup, CPU can standard luminous intensity values when debugging (this light intensity value regenerative ratio be cured among permanence storage equipment) that first reading device needs from permanence storage equipment.The controlled peak detection circuit that reflected light detecting unit comprises PIN photodiode and is connected with its output terminal, controlled peak detection circuit input end is connected with CPU, and output terminal is connected with A/D converter.Then CPU control peak detection circuit resets and is in detected state.CPU controls light source luminescent, and now, light source luminescent is irradiated in PIN diode by optical unit, and forms photocurrent, and photo-signal is linked into peak detection circuit after enlarge leadingly, and peak detection circuit can retain the peak value flowing into signal accurately.After light source luminescent terminates, CPU samples to the signal peak that peak detection circuit retains immediately.Cpu reset peak detection circuit, it is right that sampled value is carried out with standard luminous intensity values, and regulate light source voltage according to the result of contrast, thus regulate light source luminescent brightness.Preferred embodiment, this device each a period of time carries out primary calibration, and the alignment time is 6 hours.
With further reference to Fig. 3, fluorescence detection unit comprises photomultiplier and is connected controlled integrating circuit with photomultiplier output terminal, and controlled integrating circuit input end is connected with CPU, and output terminal is connected with A/D converter.CPU controls integrating circuit and resets, i.e. integrating circuit zero; Control integrating circuit and start integration, and control light source luminescent immediately; Light source activation solution to be measured, the fluorescence sent of solution is irradiated on photomultiplier minute surface and forms photocurrent, and photocurrent is linked in the middle of integrating circuit.CPU controls integrating circuit and stops integration and the integrated value controlling the controlled integrating circuit of sampling, and namely this integrated value represents the light source once luminous fluorescence total amount exciting solution to produce; CPU controls integrating circuit and resets; Preferred embodiment, because xenon lamp fluorescent lifetime is extremely short, is generally several us, but due to various time delay, the time delay of generation is generally longer than fluorescent lifetime a lot, and Change of integral time is at 50us.
The above, just better embodiment of the present utility model, but the utility model is not limited to above-described embodiment, as long as it reaches technique effect of the present utility model with any same or similar means, all should belong to protection domain of the present utility model.
Claims (5)
1. one kind can the fluorescent material concentration detection apparatus of automatically adjust light strength degree, the A/D converter comprising CPU and be connected with CPU input end, it is characterized in that, described CPU output terminal connects light source cell, reflected light detecting unit and fluorescence detection unit respectively, described reflected light detecting unit is connected with A/D converter respectively with fluorescence detection unit output terminal, and described light source is incorporated into reflected light detecting unit and fluorescence detection unit by optical module.
2. according to claim 1 a kind of can the fluorescent material concentration detection apparatus of automatically adjust light strength degree, it is characterized in that, described light source cell comprises the light source regulator and light source that connect successively.
3. according to claim 1 a kind of can the fluorescent material concentration detection apparatus of automatically adjust light strength degree, it is characterized in that, the controlled peak detection circuit that described reflected light detecting unit comprises PIN photodiode and is connected with its output terminal, described controlled peak detection circuit input end is connected with CPU, and output terminal is connected with A/D converter.
4. according to claim 1 a kind of can the fluorescent material concentration detection apparatus of automatically adjust light strength degree, it is characterized in that, described fluorescence detection unit comprises photomultiplier and is connected controlled integrating circuit with photomultiplier output terminal, described controlled integrating circuit input end is connected with CPU, and output terminal is connected with A/D converter.
5. according to claim 2 a kind of can the fluorescent material concentration detection apparatus of automatically adjust light strength degree, it is characterized in that, described light source adopts the xenon lamp of adjustable luminosity.
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CN201520808795.9U CN205139013U (en) | 2015-10-16 | 2015-10-16 | But fluorescent substance concentration detection device of automatically regulated luminous intensity |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN107449752A (en) * | 2017-07-27 | 2017-12-08 | 中绿环保科技股份有限公司 | Light source decay automatic compensating method in a kind of uv analyzer |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN107449752A (en) * | 2017-07-27 | 2017-12-08 | 中绿环保科技股份有限公司 | Light source decay automatic compensating method in a kind of uv analyzer |
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Address after: 519041 No. 19 Golden Coast Road, San Zao Town, Jin Wan District, Zhuhai, Guangdong Patentee after: Zhuhai deer Bioengineering Co.,Ltd. Address before: 519041 No. 19 Golden Coast Road, San Zao Town, Jin Wan District, Zhuhai, Guangdong Patentee before: ZHUHAI DL MEDICAL BIOTECH Co.,Ltd. |