CN107478632B - Method for detecting pH value through fluorescence of pH test paper - Google Patents
Method for detecting pH value through fluorescence of pH test paper Download PDFInfo
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- CN107478632B CN107478632B CN201710867428.XA CN201710867428A CN107478632B CN 107478632 B CN107478632 B CN 107478632B CN 201710867428 A CN201710867428 A CN 201710867428A CN 107478632 B CN107478632 B CN 107478632B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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Abstract
The invention relates to a method for detecting pH value by fluorescence of pH test paper, mainly illustrating a pH value determination method based on pH test paper and different from pH test paper color observation. When the method adopts the fluorescence of the pH test paper to realize the pH value measurement, firstly, a relation function between the fluorescence spectrum parameter of the pH test paper and the pH value of liquid is obtained as a fluorescence pH sensing equation, then, the liquid to be measured with unknown pH value is used for dipping the same type of pH test paper, the wet and developed pH test paper is dried, the fluorescence spectrum parameter of the test paper is measured, and the obtained relation function between the fluorescence spectrum parameter and the pH value of the liquid is substituted to obtain the pH value of the liquid to be measured. The invention provides a method for detecting pH value by fluorescence of pH test paper, which has the advantages that: the pH test paper used for testing can be stored for a long time and repeatedly verified; the detection process can be far away from the site where corrosive liquid exists; compared with the method for judging the color of the wet test paper, the method has higher pH measurement precision.
Description
Technical Field
The invention relates to an optical measurement method of pH value, in particular to a method for detecting the pH value through fluorescence of pH test paper.
Background
The method for measuring the pH value mainly comprises an indicator method, an electrochemical method, an optical sensor method and the like, the most common, most convenient and most cheap pH test paper belongs to the indicator method, and the operation process of the method needs to soak one piece of pH test paper with the liquid to be measured, the test paper can show the color corresponding to the pH value of the liquid to be measured, then a standard color comparison card with the same color is found through comparison, and the standard color comparison card gives the pH values corresponding to different colors. However, the test paper has unobvious color development in weak acid and alkalinity liquid, errors exist in subjective judgment of the same color, the color development index of ambient light influences judgment of the color, and the standard colorimetric card and the test paper may fade, and the accuracy of pH detection by the test paper method is low due to the factors.
The invention senses the pH value by analyzing the fluorescence spectrum parameters of the wet and dried pH test paper, the test paper can be stored for a long time, the test data can be repeatedly measured and verified, the sensing result is accurate and reliable, the pH value of weak buffer solution and low-concentration acid-base liquid can be detected, and the method is an effective supplement of the pH test paper detection technology.
Disclosure of Invention
The invention aims to provide a method for detecting pH value by fluorescence of pH test paper, which detects the fluorescence spectrum parameters of the pH test paper which is soaked in liquid to be detected and then dried to correspond to the pH value of the liquid to be detected.
The technical scheme of the invention is as follows: a method for detecting pH value by fluorescence of pH test paper is characterized in that: and dipping the pH test paper by using the liquid to be tested, drying the test paper, measuring the fluorescence spectrum parameters of the test paper, and substituting the fluorescence spectrum parameters into a relation function between the previously obtained fluorescence spectrum parameters of the pH test paper and the pH value of the liquid to obtain the pH value of the liquid to be tested.
The method for detecting the pH value through the fluorescence of the pH test paper is characterized in that: the fluorescence spectrum parameters are measured on a pH test paper which is dried after being soaked in the liquid to be measured; and the color of the pH test paper wetted by the liquid is used for roughly judging the pH value of the liquid.
The method for detecting the pH value through the fluorescence of the pH test paper is characterized in that: the steps for obtaining the relation function between the fluorescence spectrum parameter of the pH test paper and the pH value of the liquid are as follows:
respectively soaking homotype pH test paper by using liquids with known different pH values, wherein the test paper can show a color corresponding to the pH value of the liquid after being wetted;
step two, drying the soaked and wetted pH test paper;
and step three, exciting the fluorescence of the test paper, analyzing and calculating fluorescence spectrum parameters, corresponding to the pH values of the known liquid one by one, and fitting to obtain a relation function of the fluorescence spectrum parameters and the pH values of the liquid.
The method for detecting the pH value through the fluorescence of the pH test paper is characterized in that: the fluorescence spectrum parameters comprise spectrum peak wavelength, spectrum band gravity center wavelength, fluorescence intensity, fluorescence lifetime, spectrum band width and fluorescence polarization state.
The method for detecting the pH value through the fluorescence of the pH test paper is characterized in that: the pH test paper comprises wide-range pH test paper, precise pH test paper, litmus test paper, phenolphthalein test paper, Congo red test paper, fuchsin test paper and lead acetate test paper.
The invention has the beneficial effects that: a method for detecting pH value by fluorescence of pH test paper, the test paper sample tested by the method can be stored for a long time, and the traceability of test measurement is ensured; the experimental data can be repeatedly verified on the test paper sample, and the repeatability is realized; the sensing result is more accurate and reliable than the conventional use method of the pH test paper; the pH value of weak buffer solution and low-concentration acid-base liquid can be detected, and the method is an effective supplement of a pH test paper detection technology.
Drawings
Fig. 1 is a fluorescence spectrum of a dried test paper sample measured in the method for detecting pH value by fluorescence of pH test paper of the present invention, taking a wide range pH test paper dried after being soaked with standard solutions of different pH values as an example, an excitation wavelength is 405 nm.
FIG. 2 is a graph showing a relationship between a fluorescence spectrum parameter (for example, "center of band" of a spectrum parameter of a wide range pH test paper) and a pH value of the pH test paper obtained by the method for detecting a pH value by fluorescence of the pH test paper according to the present invention.
Detailed Description
The present disclosure describes, in part, a method for detecting pH by fluorescence from a pH strip. The test system used by the method can be realized based on a common grating spectrometer. Taking a commonly used wide range pH paper as an example, the specific description is as follows:
firstly, a relation function between the fluorescence spectrum parameter of the pH test paper and the pH value of the liquid is obtained as a fluorescence pH sensing equation. For the same type test paper, the obtained fluorescence pH sensing equation is generally applicable and does not need to be repeatedly obtained before the method disclosed by the invention is used for detecting the pH value of the liquid every time. The acquisition steps are as follows:
firstly, respectively and uniformly soaking liquid with different known pH values into homotypic pH test paper, and enabling the test paper to show a color corresponding to the pH value of the liquid after being wetted, so that the test paper can be compared with a test paper colorimetric card to read out a rough pH value;
drying the soaked and wetted pH test paper, wherein the drying method can be any one of natural airing, low-temperature drying, vacuum drying and the like;
selecting an excitation light source capable of exciting the fluorescence of the test paper, for example, a laser with a wavelength of 405nm, and exciting the fluorescence of the dried test paper, for example, fig. 1 shows the fluorescence spectrum of the wide-range pH test paper which is soaked in buffer solutions with different pH values (pH range of 4-9) and dried under the excitation of the laser with the wavelength of 405 nm. Analyzing and calculating fluorescence spectrum parameters, such as peak wavelength, band center of gravity wavelength, fluorescence intensity, fluorescence lifetime, band width and fluorescence polarization state, corresponding the obtained fluorescence spectrum parameters to the pH value of the known liquid one by one, and fitting to obtain a relation function of the fluorescence spectrum parameters and the pH value of the liquid, for example, fig. 2 is a relation function curve of the fluorescence spectrum parameters-band center of gravity wavelength-and the pH value of a wide-range pH test paper soaked in a pH buffer solution and dried in the air.
Then, dipping the same type pH test paper in a liquid to be measured with unknown pH value, drying the wet developed pH test paper, exciting the dipped and dried pH test paper with the exciting light, measuring the fluorescence spectrum parameters of the test paper, such as the band center of gravity wavelength, substituting the obtained fluorescence spectrum parameters into the relation function of the liquid pH value, such as the fluorescence pH sensing equation in FIG. 2, and finally obtaining the pH value of the liquid to be measured.
Claims (5)
1. A method for detecting pH value by fluorescence of pH test paper is characterized in that: and dipping the pH test paper by using the liquid to be tested, drying the test paper, measuring the fluorescence spectrum parameters of the test paper, and substituting the fluorescence spectrum parameters into a relation function between the previously obtained fluorescence spectrum parameters of the pH test paper and the pH value of the liquid to obtain the pH value of the liquid to be tested.
2. The method of claim 1, wherein the pH is detected by fluorescence from a pH strip, wherein: the fluorescence spectrum parameters are measured on a pH test paper which is dried after being soaked in the liquid to be measured; and the color of the pH test paper wetted by the liquid is used for roughly judging the pH value of the liquid.
3. The method of claim 1, wherein the pH is detected by fluorescence from a pH strip, wherein: the steps for obtaining the relation function between the fluorescence spectrum parameter of the pH test paper and the pH value of the liquid are as follows:
respectively soaking homotype pH test paper by using liquids with known different pH values, wherein the test paper can show a color corresponding to the pH value of the liquid after being wetted;
step two, drying the soaked and wetted pH test paper;
and step three, exciting the fluorescence of the test paper, analyzing and calculating fluorescence spectrum parameters, corresponding to the pH values of the known liquid one by one, and fitting to obtain a relation function of the fluorescence spectrum parameters and the pH values of the liquid.
4. The method of claim 1, wherein the pH is detected by fluorescence from a pH strip, wherein: the fluorescence spectrum parameters comprise spectrum peak wavelength, spectrum band gravity center wavelength, fluorescence intensity, fluorescence lifetime, spectrum band width and fluorescence polarization state.
5. The method of claim 1, wherein the pH is detected by fluorescence from a pH strip, wherein: the pH test paper comprises wide-range pH test paper, precise pH test paper, litmus test paper, phenolphthalein test paper, Congo red test paper, fuchsin test paper and lead acetate test paper.
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| CN109232397B (en) * | 2018-09-19 | 2020-04-28 | 山东大学 | Alkaline precise pH test paper capable of being repeatedly utilized through washing and preparation method and application thereof |
| CN114002217A (en) * | 2021-11-02 | 2022-02-01 | 吉林市国科医工科技发展有限公司 | Method for reading PH value through PH test paper image |
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| CN1959381A (en) * | 2006-11-13 | 2007-05-09 | 湖南大学 | Fluorescence PII sensor with inner reference and comparison, and preparation method |
| CN101424641A (en) * | 2008-11-01 | 2009-05-06 | 山西大同大学 | Strong acid type ph fluorescent probe based on fluoro-boron fluorescent dye |
| CN102320991A (en) * | 2011-08-17 | 2012-01-18 | 南阳师范学院 | New molecule of PH fluorescent probe |
| CN102495049A (en) * | 2011-12-09 | 2012-06-13 | 南京大学 | Optical ion sensing membrane for detecting pH and preparation method and application thereof |
| CN103336007A (en) * | 2013-06-08 | 2013-10-02 | 中国医学科学院基础医学研究所 | Portable paper chip capable of visually detecting chlorine ion content in sweat |
| CN104792759A (en) * | 2015-04-28 | 2015-07-22 | 济南大学 | Fluorescent test paper capable of rapidly detecting formaldehyde and application of fluorescent test paper |
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- 2017-09-22 CN CN201710867428.XA patent/CN107478632B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1959381A (en) * | 2006-11-13 | 2007-05-09 | 湖南大学 | Fluorescence PII sensor with inner reference and comparison, and preparation method |
| CN101424641A (en) * | 2008-11-01 | 2009-05-06 | 山西大同大学 | Strong acid type ph fluorescent probe based on fluoro-boron fluorescent dye |
| CN102320991A (en) * | 2011-08-17 | 2012-01-18 | 南阳师范学院 | New molecule of PH fluorescent probe |
| CN102495049A (en) * | 2011-12-09 | 2012-06-13 | 南京大学 | Optical ion sensing membrane for detecting pH and preparation method and application thereof |
| CN103336007A (en) * | 2013-06-08 | 2013-10-02 | 中国医学科学院基础医学研究所 | Portable paper chip capable of visually detecting chlorine ion content in sweat |
| CN104792759A (en) * | 2015-04-28 | 2015-07-22 | 济南大学 | Fluorescent test paper capable of rapidly detecting formaldehyde and application of fluorescent test paper |
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