CN210090307U - Time-dependent fluorescence testing device - Google Patents
Time-dependent fluorescence testing device Download PDFInfo
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- CN210090307U CN210090307U CN201920351071.4U CN201920351071U CN210090307U CN 210090307 U CN210090307 U CN 210090307U CN 201920351071 U CN201920351071 U CN 201920351071U CN 210090307 U CN210090307 U CN 210090307U
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Abstract
The utility model discloses a time-dependent fluorescence testing device, which comprises an excitation light source, an optical fiber collimator, an optical filter, a convex lens, an air guide pipeline, an integrating sphere, a standard light source, an optical fiber spectrometer, a computer and optical fibers; an excitation light source is connected with an optical fiber collimator through an optical fiber to complete light source beam expansion, and enters an integrating sphere through a band-pass filter and a convex lens in sequence, a diffuse reflection layer with high reflectivity is coated on the inner wall of the integrating sphere, a sample to be detected is placed in the center of the integrating sphere through a clamp, and a detection port is formed in the integrating sphere and used for coupling connection of an optical fiber probe of an optical fiber spectrometer. The integrating sphere is internally provided with the standard light source, so that the measurement errors caused by different positions of the standard light source during light intensity calibration can be avoided. Meanwhile, the integrating sphere is additionally provided with a small vent hole for introducing a trace amount of inert gas, so that measurement errors caused by the influence of oxygen in the air on the sample during measurement are avoided.
Description
Technical Field
The utility model belongs to the photochemistry field, concretely relates to time-dependent fluorescence testing arrangement, promptly: a measuring device for measuring the change of fluorescence of luminescent materials or devices with time under the condition of optical excitation.
Background
In recent years, with the rapid development of light emitting diode lighting devices, research on luminescent materials is receiving more and more attention, and fluorescence attenuation is receiving much attention as an important index for evaluating the stability of luminescent materials. Fluorescence decay measurements provide an opportunity to observe the excited state behavior of the microsystem, providing conditions for the development of microsystem interactions and for the study of the intrinsic connection to macroscopic properties.
The luminous intensity of a to-be-measured luminous sample is often characterized by uneven spatial distribution due to factors such as internal chemical components, external dimensions and the like, and therefore, the prior art and related measuring devices adopt an integrating sphere to collect fluorescence emitted from a measuring object. In the prior art, in order to ensure the accuracy of the test, a testing device regularly adopts a standard light source for calibration, the standard light source is usually arranged at a fixed position in an integrating sphere for calibration, and the standard light source can be taken out after the calibration is finished. The method has the defect that no standard light source exists in the integrating sphere when a sample is measured, so that the environment in the integrating sphere during calibration is different from the environment in the integrating sphere during measurement, and experimental errors are generated.
In addition, in the prior art, the measurement of the change of fluorescence of the luminescent material along with time is carried out in the air, the material is in a measurement state for a long time during the test, and for some materials, oxygen in the air can have great damage effect on the material; other materials, such as phosphorescent materials, oxygen in air can quench the luminescence of the materials; at this time, the data obtained by the prior art cannot correctly characterize the photoluminescence performance of the material and the real process of the change with time under the light excitation.
Disclosure of Invention
The utility model discloses the technical problem that will solve is: different and the air in the air measurement can cause technical problem such as certain error to the test result around the standard light source is revised to exist among the prior art, the utility model provides a relevant fluorescence testing arrangement of test time.
In order to solve the technical problems, the invention adopts the following technical scheme:
providing a time-dependent fluorescence testing device, the device comprising: the device comprises an excitation light source, an optical fiber collimator, an optical filter, a convex lens, an air guide pipeline, an integrating sphere, a standard light source, an optical fiber spectrometer, a computer and an optical fiber; the excitation light source is directly connected with the optical fiber collimator or connected with the optical fiber collimator through an optical fiber, the excitation light emitted by the excitation light source passes through the optical fiber collimator and expands the beam to enter the integrating sphere in the air through the optical filter and the convex lens in sequence, and the integrating sphere is connected with the optical fiber spectrometer; the integrating sphere is connected with the air guide pipeline, a standard light source is arranged in the integrating sphere, an optical fiber probe of the optical fiber spectrometer is positioned on the inner wall of the integrating sphere, and the information output end of the optical fiber spectrometer is connected with the computer.
Preferably, the integrating sphere is provided with an air guide micropore, and the integrating sphere is connected with the air guide pipeline through the air guide micropore.
Preferably, the integrating sphere is provided with a variety of sample holders to satisfy the test for solid samples, solution samples, film samples and powder samples.
Preferably, the excitation light source can adopt a monochromatic light source as the excitation light source, and the test of the wide-range luminescent material is completed by changing the combination between the light source and the optical filter.
Preferably, the inner wall of the integrating sphere is coated with a diffuse reflection layer.
Preferably, the diffuse reflection layer on the inner wall of the integrating sphere is made of inorganic salt and modified materials thereof, or polyfluoro plastics and modified materials thereof.
Preferably, the diffuse reflection layer on the inner wall of the integrating sphere is made of barium sulfate and polytetrafluoroethylene.
Preferably, the integrating sphere is provided with a micro-hole for allowing excitation light to enter the integrating sphere.
Preferably, the device also comprises a flowmeter, and the flowmeter is connected with the air guide pipeline.
Preferably, the excitation light source adopts a monochromatic LED bulb as the excitation light source.
Compared with the prior art, the time-dependent fluorescence testing device has the following beneficial results:
1. the measurement of the fluorescence attenuation of the luminescent material in any wave band can be satisfied by adopting the monochromatic light source as the excitation light source and adopting the monochromatic light sources with different wavelengths as the excitation light source.
2. The testing device has the advantages that the standard light source is arranged in the integrating sphere, so that the internal environment of the integrating sphere during measurement is ensured to be the same as the internal environment of the integrating sphere during correction, and the testing error is reduced.
3. This testing arrangement is equipped with inert gas pipe, can let in inert gas when the test, and the experimental environment that the sample was located when guaranteeing the test reduces the influence of air to the sample, improves measurement accuracy.
Drawings
FIG. 1 is a schematic diagram of a time-dependent fluorescence testing apparatus according to the present invention;
FIG. 2 is a schematic diagram of an integrating sphere of the time-dependent fluorescence testing apparatus according to the present invention;
FIG. 3 is a graph of photoluminescence attenuation data obtained from a time-dependent fluorescence testing apparatus test (IAIQ) according to the present invention;
FIG. 4 is a screenshot of a spectrum obtained from a time-dependent fluorescence tester test (IAIQ) according to the present invention; a 5 minute screenshot (left) and a 100 minute screenshot (right);
FIG. 5 is a graph showing the fluorescence attenuation change of a series of 223 phosphorescent luminescent materials doped with polystyrene with a molecular weight of 100 ten thousand at a concentration of 5% under the irradiation of ultraviolet light of 375 nm;
fig. 6 is a graph illustrating the detection of the variation of the IAIQ material photoluminescence enhancement using the device of the present invention.
Reference numerals: 1-an excitation light source; 2-a fiber collimator; 3-optical filter; a 4-convex lens; 5-an air guide pipeline; 6-integrating sphere; 7-sample holder; 8-standard light source; 9-fiber optic spectrometer; 10-a computer; 11-an optical fiber; 12-sample to be tested.
Detailed Description
The utility model provides a time-dependent fluorescence testing arrangement, as shown in figure 1 and figure 2, the device includes: an excitation light source 1, an optical fiber collimator 2, a filter 3, a convex lens 4, an air guide pipeline 5, an integrating sphere 6, a sample clamp 7, a standard light source 8, an optical fiber spectrometer 9, a computer 10 and an optical fiber 11; an excitation light source 1 is directly connected with an optical fiber collimator 2 or is connected with the optical fiber collimator 2 through an optical fiber 11, the excitation light is expanded through the optical fiber collimator 2 and then enters an integrating sphere 6 in the air through an optical filter 3 and a convex lens 4 in sequence, and the integrating sphere 6 is connected with an optical fiber spectrometer; the integrating sphere 6 is provided with gas guide micropores and is connected with the gas guide pipeline 5 for introducing inert gas inside the integrating sphere 6, and the inert gas is introduced for a period of time before testing, so that the gas atmosphere of the environment where the sample is located during measurement is ensured, the influence of oxygen on the sample is reduced, and the testing precision is improved. The integrating sphere 6 is internally provided with a standard light source 8 to ensure that the internal environment of the integrating sphere is consistent with that of the integrating sphere during calibration when a sample is measured, so that the measurement error caused by the change of the internal environment of the integrating sphere is avoided. The optical fiber probe of the optical fiber spectrometer 9 is positioned on the inner wall of the integrating sphere 6, and the information output end of the optical fiber spectrometer 9 is connected with the computer 10.
In addition, the excitation light source 1 can adopt a monochromatic light source as the excitation light source, and the test of the wide-range luminescent material is completed by changing the combination between the light source and the optical filter; the integrating sphere 6 is provided with various sample holders 7 to satisfy the test of the solution sample and the film sample. The inner wall of the integrating sphere 6 is coated with a diffuse reflection layer with high reflectivity (the material can be inorganic salt and modified materials thereof, such as barium sulfate and the like, or polyfluoro plastics and modified materials thereof, such as polytetrafluoroethylene and the like). The integrating sphere is provided with a micro-hole for exciting light to enter the integrating sphere.
The invention will be described in further detail with reference to the drawings and specific examples
Example 1 method of Using a time-dependent fluorescence test device
When a time-dependent fluorescence testing device is used, after a standard light source is used for correction, inert gas is firstly introduced into an integrating sphere 6 through a gas conduit 5; then scanning the environmental background by using a fiber spectrometer 9; then, after an excitation light source 1 is turned on to wait for the light source to be stable, a sample 12 to be detected is placed in a sample clamp 7, then the device is kept still, light emitted by the excitation light source 1 is expanded through an optical fiber collimator 2, then the light is limited through an optical filter 3 to improve monochromaticity, then the light is converged through a convex lens 4 and enters an integrating sphere 6, and a generated optical signal is transmitted into an optical fiber spectrometer 9 through an optical fiber 11 and is analyzed through a computer 10, and the change of the spectrum along with time is automatically recorded.
Example 2
The test materials were tested in liquid and solid states using the apparatus described in the present model, using the method described in example 1. The decay of fluorescence under strong excitation light irradiation was tested, and the light-to-light emission decay data of the obtained test material is shown in fig. 3. Where the ordinate represents the proportion of laser radiation at a fixed wavelength and intensity relative to the starting device and material and the abscissa represents time. Where the spectrometer reads the data directly. The ordinate data reflects fluorescence quantum intensity, and the value is the number of light quanta obtained by integrating and converting the wavelength of abscissa in the spectrum intensity read by the spectrometer, so that the change of the number of the light quanta generated by the device under a fixed condition along with time can be represented. FIG. 4 reflects the raw spectra of the test material at various times, as can be seen from the intensity on the ordinate, the material has a decay in fluorescence under illumination.
Example 3
The 223 series phosphorescent luminescent materials are doped into polystyrene with 100 ten thousand molecular weight at the concentration of 5 percent and irradiated by ultraviolet light at 375nm, and the change of the number of luminescent photons of the luminescent molecules along with time is read by a spectrometer and a computer. Therefore, the fluorescence attenuation change contrast of the series of materials under the same strong laser irradiation is obtained, and a proper material can be selected according to the obtained data, and the test result is shown in fig. 5.
Example 4
Testing the spectrum change condition of the special photoelectric functional material under the condition of constant illumination. As shown in FIG. 6, the 50% IAIQ doped PMMA film shows a first-increase and then-decay process in the time-varying light from orange to red under irradiation of a specific wavelength. According to the obtained spectrum, the emission spectrum reaches a maximum value when the sample is irradiated for 1200 seconds, and then the emission shows a tendency of decay.
The above examples 2-4 illustrate that the device can be used to test the spectrum of a time varying light excitation, the change in the number of luminescent photons, and further can be used to measure the decay of phosphorescent materials and devices under light conditions. So that the method can be used for analyzing the light-to-light-emitting stability of the material and analyzing the mechanism of light-to-light-emitting enhancement of a special material.
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (10)
1. A time-dependent fluorescence testing device, comprising: the device comprises an excitation light source (1), an optical fiber collimator (2), a filter (3), a convex lens (4), an air guide pipeline (5), an integrating sphere (6), a standard light source (8), an optical fiber spectrometer (9), a computer (10) and an optical fiber (11); an excitation light source (1) is directly connected with an optical fiber collimator (2) or is connected with the optical fiber collimator (2) through an optical fiber (11), the excitation light emitted by the excitation light source passes through the optical fiber collimator (2) and is expanded to the back, the excitation light sequentially passes through a light filter (3) and a convex lens (4) in the air and enters an integrating sphere (6), and the integrating sphere (6) is connected with an optical fiber spectrometer (9); the integrating sphere (6) is connected with the air guide pipeline (5), the integrating sphere (6) is internally provided with a standard light source (8), an optical fiber probe of the optical fiber spectrometer (9) is positioned on the inner wall of the integrating sphere (6), and the information output end of the optical fiber spectrometer (9) is connected with the computer (10).
2. The time-dependent fluorescence test device of claim 1, wherein the integrating sphere (6) is provided with an air guide micropore, and the integrating sphere (6) is connected with the air guide duct (5) through the air guide micropore.
3. The time-dependent fluorescence testing device of claim 1, wherein the integrating sphere (6) is provided with a plurality of sample holders (7) for satisfying the test of solid samples, solution samples, film samples and powder samples.
4. The fluorescence testing apparatus according to claim 1, wherein the excitation light source (1) can be a monochromatic light source, and the testing of the wide range of luminescent materials is performed by changing the combination between the light source and the filter.
5. A time-dependent fluorescence testing device according to claim 1, wherein the inner wall of the integrating sphere (6) is coated with a diffuse reflective layer.
6. A time-dependent fluorescence testing device according to claim 5, wherein the diffuse reflective layer material on the inner wall of the integrating sphere (6) is inorganic salt or polyfluoro plastic.
7. The time-dependent fluorescence testing device of claim 6, wherein the diffuse reflective layer material on the inner wall of the integrating sphere (6) is barium sulfate and polytetrafluoroethylene.
8. A time-dependent fluorescence testing device according to claim 1, wherein the integrating sphere is provided with a micro-hole for enabling excitation light to enter the integrating sphere (6).
9. A time-dependent fluorescence testing device according to claim 1, characterized in that the device further comprises a flow meter connected to the gas conducting duct (5).
10. The time-dependent fluorescence testing device according to claim 4, wherein the excitation light source (1) is a monochromatic LED bulb.
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| CN201920351071.4U CN210090307U (en) | 2019-03-19 | 2019-03-19 | Time-dependent fluorescence testing device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109827936A (en) * | 2019-03-19 | 2019-05-31 | 南京佳诺霖光电科技有限公司 | A kind of time correlation fluorescence test apparatus |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109827936A (en) * | 2019-03-19 | 2019-05-31 | 南京佳诺霖光电科技有限公司 | A kind of time correlation fluorescence test apparatus |
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