CN201740737U - Integrating sphere fluoroscopic detection device based on LED light source - Google Patents
Integrating sphere fluoroscopic detection device based on LED light source Download PDFInfo
- Publication number
- CN201740737U CN201740737U CN2010201949957U CN201020194995U CN201740737U CN 201740737 U CN201740737 U CN 201740737U CN 2010201949957 U CN2010201949957 U CN 2010201949957U CN 201020194995 U CN201020194995 U CN 201020194995U CN 201740737 U CN201740737 U CN 201740737U
- Authority
- CN
- China
- Prior art keywords
- integrating sphere
- base
- light source
- pass filter
- narrow band
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The utility model relates to an integrating sphere fluoroscopic detection device based on an LED light source. The conventional detection device has the disadvantage that the fluoroscopic intensity detection is influenced due to unsteady lighting. A sample holder is arranged in an integrating sphere; a first round through hole is formed at the maximum horizontal cross-section of the integrating sphere; a first base is arranged on the integrating sphere in the position corresponding to the outer part of the first round through hole; an LED is arranged in the center at the closed end of the first base; a first narrow band pass filter is arranged near the opened end of the first base; a second round through hole is formed over the integrating sphere; a second base is arranged on the integrating sphere in a position corresponding to the outer part of the second round through hole; a photomultiplier is arranged in the center at one end of the second base far away from the integrating sphere; a second narrow band pass filter is arranged near the other end of the integrating sphere; an optical fiber penetrates the integrating sphere; one end of the optical fiber is flush with the inner side wall of the integrating sphere; and the other end of the optical fiber is connected with a fluorescence spectrophotometer. The simplicity and high radiant intensity of an excitation light source are effectively guaranteed, so that the light source is kept within a narrower wavelength range.
Description
Technical field
The utility model belongs to optics and biotechnology crossing domain, relates to a kind of fluorescence detection device, especially a kind of integrating sphere type fluorescence detection device based on led light source.
Background technology
Utopian excitation source should possess in the fluoroscopic examination instrument: 1. enough intensity; 2. continuous spectrum arranged in required spectral range; 3. its intensity and Wavelength-independent, i.e. the output of light source should be the equicohesive radiation of continuously smooth; 4. light intensity will be stablized.But the light source that in fact, meets these conditions does not exist.And the fluoroscopic examination instrument is a kind of high-sensitivity analysis instrument, though can obtain observable signal by the gain or the enlargement factor that improve instrument, but the unstable rule of excitation source can have influence on intensity of fluorescence, thereby can't obtain reliable spectroscopic data.
At present, the excitation source of fluorescent instrument adopts xenon lamp, mercury lamp, tungsten lamp and laser instrument more.Xenon lamp, mercury lamp and tungsten lamp all are the widely used light sources of early stage fluorescent instrument, and at various wave bands radiation are arranged all, but in different wave band output signal strength differences, need add monochromator and optical filter thereafter.Laser instrument is the light source that present high-performance fluorescent instrument mainly uses, the laser instrument of single wavelength, can excite high-intensity light source, and monochromaticity is good, do not have parasitic light, tunable laser instrument can remedy the deficiency that mono-colour laser has only a kind of wavelength light, can change under several wavelength, but the wavelength range of choice is little, and price and costliness thereof.
Summary of the invention
In order to overcome excitation source luminous intensity instability in the existing fluorescence detection device, the deficiency of monochromaticity difference, the utility model provides a kind of integrating sphere type fluorescence detection device based on led light source.This device can provide spectral range narrower monochromatic light under lower cost, improves the stability and the radiation intensity of excitation source effectively.The utility model is to realize by following technical scheme:
A kind of integrating sphere type fluorescence detection device based on led light source comprises LED, first narrow band pass filter, first base, second narrow band pass filter, second base, integrating sphere, sample holder, photomultiplier and optical fiber.
Sample holder is set in the integrating sphere, the maximum horizontal section place of integrating sphere has first manhole, integrating sphere is to being provided with first base outward by through hole, described first base is that an end is open, the tubular construction of other end sealing, the blind end center of first base is provided with LED, and the emergent light of LED is over against the centre of sphere; First narrow band pass filter is arranged on the open end near first base;
Have second manhole directly over the integrating sphere, integrating sphere is to being provided with second base outward by through hole, described second base is the cylinder of both ends open, and the end center away from integrating sphere of second base is provided with photomultiplier, photomultiplier over against centre of sphere setting; Second narrow band pass filter is arranged on the other end near integrating sphere;
Optical fiber runs through the integrating sphere setting, and an end of optical fiber flushes with the integrating sphere madial wall, another termination fluorescence spectrophotometer.
Described sample holder comprises support bar, pallet and positioning disk, many highly identical support bars are symmetrical arranged around the axis perpendicular to surface level, positioning disk is positioned at the plane that supporting bar top is enclosed, and pallet and be arranged in parallel with positioning disk between positioning disk and integrating sphere; The center of described positioning disk has pilot hole.The corresponding pilot hole of described pallet place is a concave surface.
The utility model is compared with background technology, and the useful effect that has is:
1, adopt monochromatic LED that light source is provided, can guarantee the unicity and the high radiance of excitation source effectively, add narrow band filter slice again, energy elimination parasitic light remains in the narrower wavelength coverage light source.
2, the structure that adopts excitation source to be connected with integrating sphere can make the exciting light radiation more level and smooth, has reduced the influence of the instability of excitation source for sample.
3, the mode that adopts photomultiplier and integrating sphere to be connected at the top, thus the fluorescence that sample produced in the test tube is directly injected in the photomultiplier.
4, adopt parts such as monochromatic LED, narrow band pass filter, integrating sphere and photomultiplier, can effectively reduce instrument cost, and omit supply unit and monochromator, reduced the volume of fluorescence detection device, improve the cost performance of instrument.
5, adopt checkout equipments such as photomultiplier and spectral investigator, can make the excited fluorescent testing result more accurate, photomultiplier can be accepted light intensity signal, can measure the fluorescence light intensity effectively by follow-up amplifying circuit, and fluorescence spectrophotometer then can detect the various parameters of fluorescence.
Description of drawings
Fig. 1 is a structural representation of the present utility model;
Fig. 2 moves towards synoptic diagram for optical fiber in the testing process;
Fig. 3 is a photomultiplier subsequent conditioning circuit block diagram.
Embodiment
The utility model is described in further detail below in conjunction with accompanying drawing.
Shown in 1 figure, a kind of integrating sphere type fluorescence detection device based on led light source comprises monochromatic LED 6, first narrow band pass filter 7, first base 5, second narrow band pass filter 4, second base 3, integrating sphere 8, sample holder 10, photomultiplier 2 and optical fiber 1.
Integrating sphere 8 oblique settings, sample holder 10 is set in the integrating sphere 8, the maximum horizontal section place of integrating sphere 8 has first manhole, integrating sphere is to being provided with first base 5 outward by through hole, first base 5 is that an end is open, the tubular construction of other end sealing, the blind end center of first base 5 is provided with monochromatic LED 6, and the emergent light of LED is over against the centre of sphere; First narrow band pass filter 7 is arranged on the open end near first base 5;
Have second manhole directly over the integrating sphere 8,8 pairs of integrating spheres should be provided with second base 3 outward by through hole, second base 3 is the cylinder of both ends open, and the end center away from integrating sphere of second base 3 is provided with photomultiplier 2, photomultiplier 2 over against centre of sphere setting; Second narrow band pass filter 7 is arranged on the other end near integrating sphere;
As shown in Figure 2, the concrete steps that the utility model detects are: open integrating sphere, sample tube to be measured is put into pilot hole, by tray support.The light that LED sends is by entering integrating sphere after the first narrow band pass filter elimination parasitic light, makes the light intensity of light reach uniform distribution through diffuse reflection repeatedly.After uniform irradiate light is to the sample tube, excited fluorescent material, make it to produce fluorescence.The fluorescence that sample produces shines the photomultiplier that is connected with integrating sphere second light hole by second narrow band pass filter.Be placed in the top of test tube because of photomultiplier, integrating sphere is irreflexive to be influenced so fluorescence will can not be subjected to, directly enter photomultiplier, by after drive circuit module, little current/voltage-converted circuit module, signal conditioning circuit module, negative high voltage module and the effect of output circuit module of photomultiplier subsequent conditioning circuit (as shown in Figure 3), read the signal of light intensity again.Side at photomultiplier is connected with optical fiber, with after spectral investigator is connected, is used to detect the spectral range of fluorescence by optical fiber.
Claims (2)
1. the integrating sphere type fluorescence detection device based on led light source comprises integrating sphere, LED, first narrow band pass filter, first base, second narrow band pass filter, second base, sample holder, photomultiplier and optical fiber, it is characterized in that:
The oblique setting of integrating sphere, sample holder is set in the integrating sphere, the maximum horizontal section place of integrating sphere has first manhole, integrating sphere is to being provided with first base outward by through hole, described first base is that an end is open, the tubular construction of other end sealing, the blind end center of first base is provided with LED, and the emergent light of LED is over against the centre of sphere; First narrow band pass filter is arranged on the open end near first base;
Have second manhole directly over the integrating sphere, integrating sphere is to being provided with second base outward by through hole, described second base is the cylinder of both ends open, and the end center away from integrating sphere of second base is provided with photomultiplier, photomultiplier over against centre of sphere setting; Second narrow band pass filter is arranged on the other end near integrating sphere;
Optical fiber runs through the integrating sphere setting, and an end of optical fiber flushes with the integrating sphere madial wall, another termination fluorescence spectrophotometer.
2. a kind of integrating sphere type fluorescence detection device according to claim 1 based on led light source, it is characterized in that: described sample holder comprises support bar, pallet and positioning disk, many highly identical support bars are symmetrical arranged around the axis perpendicular to surface level, positioning disk is positioned at the plane that supporting bar top is enclosed, pallet and be arranged in parallel with positioning disk between positioning disk and integrating sphere; The center of described positioning disk has pilot hole, and the corresponding pilot hole of described pallet place is a concave surface.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010201949957U CN201740737U (en) | 2010-05-18 | 2010-05-18 | Integrating sphere fluoroscopic detection device based on LED light source |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010201949957U CN201740737U (en) | 2010-05-18 | 2010-05-18 | Integrating sphere fluoroscopic detection device based on LED light source |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201740737U true CN201740737U (en) | 2011-02-09 |
Family
ID=43556014
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010201949957U Expired - Fee Related CN201740737U (en) | 2010-05-18 | 2010-05-18 | Integrating sphere fluoroscopic detection device based on LED light source |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201740737U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103115911A (en) * | 2013-03-05 | 2013-05-22 | 中国原子能科学研究院 | Closed type fluorescence analyzer |
| CN103335710A (en) * | 2013-07-11 | 2013-10-02 | 北京半导体照明科技促进中心 | Carrier device, light flux test system and light flux test method of flexible LED lamp bar |
| CN104457994A (en) * | 2014-12-09 | 2015-03-25 | 苏州科利亚照明科技有限公司 | Light analyzer |
| CN110426380A (en) * | 2019-09-29 | 2019-11-08 | 常州星宇车灯股份有限公司 | A kind of test device of the laser excitation remote fluorescence material of transmission-type controllable temperature |
-
2010
- 2010-05-18 CN CN2010201949957U patent/CN201740737U/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103115911A (en) * | 2013-03-05 | 2013-05-22 | 中国原子能科学研究院 | Closed type fluorescence analyzer |
| CN103335710A (en) * | 2013-07-11 | 2013-10-02 | 北京半导体照明科技促进中心 | Carrier device, light flux test system and light flux test method of flexible LED lamp bar |
| CN103335710B (en) * | 2013-07-11 | 2015-11-25 | 常州市武进区半导体照明应用技术研究院 | The carrier arrangement of flexible LED lamp bar and luminous flux test macro and method |
| CN104457994A (en) * | 2014-12-09 | 2015-03-25 | 苏州科利亚照明科技有限公司 | Light analyzer |
| CN110426380A (en) * | 2019-09-29 | 2019-11-08 | 常州星宇车灯股份有限公司 | A kind of test device of the laser excitation remote fluorescence material of transmission-type controllable temperature |
| CN110426380B (en) * | 2019-09-29 | 2020-01-21 | 常州星宇车灯股份有限公司 | Transmission-type temperature-controllable testing device for laser-excited remote fluorescent material |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101825571A (en) | Integrating sphere type fluorescence detection device based on LED light source | |
| CA3034107C (en) | Methods and systems for determining the presence of a molecule in a sample and method and system for determining a range-resolved concentration of a molecule in a scene | |
| Bykov et al. | Steady-state and transient ultraviolet resonance Raman spectrometer for the 193–270 nm spectral region | |
| DeRose et al. | Recommendations for fluorescence instrument qualification: the new ASTM Standard Guide | |
| CN201740737U (en) | Integrating sphere fluoroscopic detection device based on LED light source | |
| CN103344613B (en) | A kind of material reflection characteristic measurement apparatus and method | |
| US20130301051A1 (en) | Scattering light source multi-wavelength photometer | |
| CN104458634A (en) | Pulsed multi-channel photoacoustic spectrometry device for gas detection | |
| JPS6116010B2 (en) | ||
| JP2009008509A (en) | Light emission quantum efficiency measuring device | |
| CN108088832A (en) | A kind of single light source CARS spectral devices and the method for detecting Raman active medium | |
| CN206945540U (en) | A kind of efficient detection device based on Laser-induced plasma spectroscopy | |
| Drobizhev et al. | Characterizing the two-photon absorption properties of fluorescent molecules in the 680-1300 nm spectral range | |
| CN106066317A (en) | Optical chopper using method in delayed luminescence measurement system | |
| CN210834097U (en) | Optical test system | |
| CN201514381U (en) | Laser Raman spectrometer with surface-enhanced sampler | |
| CN102519920A (en) | Ultraviolet and deep ultraviolet optical thin film element double-wavelength laser fluorescence spectrometer | |
| CN201514316U (en) | Solar battery spectral response tester | |
| JP2008518222A (en) | Relative method for measuring the fluorescence quantum yield of dyes in solution. | |
| CN214097162U (en) | Fluorescence emission spectrum and service life detection system | |
| KR20220080183A (en) | System and method for vacuum ultraviolet lamp assisted ignition of oxygen-containing laser sustained plasma source | |
| CN201137890Y (en) | Ground ultra-violet radiation scaling standard light source | |
| CN109991197A (en) | Measure the spectrophotometer and method of fluorescent characteristic | |
| CN112414959A (en) | Small-sized high-sensitivity spectrophotometer | |
| CN201335808Y (en) | Double-wavelength optical detection device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110209 Termination date: 20110518 |