CN214097162U - Fluorescence emission spectrum and service life detection system - Google Patents
Fluorescence emission spectrum and service life detection system Download PDFInfo
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- CN214097162U CN214097162U CN202022985207.2U CN202022985207U CN214097162U CN 214097162 U CN214097162 U CN 214097162U CN 202022985207 U CN202022985207 U CN 202022985207U CN 214097162 U CN214097162 U CN 214097162U
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Abstract
The utility model discloses a fluorescence emission spectrum and life-span detection system relates to fluorescence technical field, and this fluorescence emission spectrum and life-span detection system are including inverting optical microscope, objective, total reflection mirror, shell, focusing mirror, adjustable pinhole, beam expanding mirror, fiber coupler, multimode fiber, monochromator, single photon detector, two to the mirror module, periscope group, diaphragm, adjustable neutral filter group and pulse laser. The embodiment of the utility model provides a fluorescence emission spectrum combines together fluorescence life-span formation of image and fluorescence wavelength detection with life-span detection system, further follows the attribute of two kinds of different dimensions simultaneous study objects, surveys simultaneously through having fused two kinds of parameters of fluorescence life-span and fluorescence wavelength, has integrateed the high spatial resolution who confocal to and the accurate screening of monochromator to the wavelength, has extended the reference dimension for studying object light physics chemical properties, thereby has promoted the reliability and the comprehensive nature of experiment.
Description
Technical Field
The utility model relates to a fluorescence technology field especially relates to a fluorescence emission spectrum and life-span detection system.
Background
The fluorescence emitted by the object contains various photophysical properties. For example, the uniformity of a single-molecule coating film, the photoelectric conversion and storage efficiency of a photovoltaic solar cell material, the energy transfer between molecules, the configuration of macromolecular protein, the material transfer characteristic in cells and the like are judged. Can be effectively quantified and calibrated by measuring the fluorescence lifetime of the fluorescent material. Fluorescence is used as light energy and is converted from other energy in the same or different forms, the common form is that the light energy is converted with each other, the sample is stimulated by the short-wavelength light energy with higher energy, and the sample emits the long-wavelength fluorescence with lower energy through energy level transition and internal energy conversion mechanisms, and the experimental mode is common at present. In addition, since the wavelength is one of the fundamental properties of light energy, many research fields pay attention to the wavelength of fluorescence emitted by an object, especially for the field of materials science, quantitative data acquisition and analysis of two dimensions of the lifetime and the wavelength are needed to be simultaneously carried out on the fluorescence emitted by a sample in the same area, so as to research the performance of the material and improve the corresponding production process. It is noted that spectral splitting further screens the fluorescence emitted by the sample, in which case the intensity of the fluorescence signal is reduced to a greater extent. Therefore, there is a need for a detection system that can simultaneously detect both fluorescence lifetime and fluorescence wavelength parameters.
SUMMERY OF THE UTILITY MODEL
In order to overcome the defects of related products in the prior art, the utility model provides a fluorescence light-emitting spectrum and service life detection system.
The utility model provides a fluorescence emission spectrum and life-span detection system, include: the system comprises an inverted optical microscope, wherein an objective lens is arranged under a sample stage of the inverted optical microscope, and a sample for fluorescence detection is fixedly placed on the sample stage corresponding to the position of the objective lens; a total reflection mirror is arranged right below the objective lens; a light-blocking shell is arranged on one side of the inverted optical microscope, a monochromator and a single-photon detector are sequentially arranged on the outer side of the shell, a focusing lens, an adjustable pinhole, a beam expanding lens and an optical fiber coupler are arranged in the shell, the total reflection lens, the focusing lens, the adjustable pinhole, the beam expanding lens and the optical fiber coupler are sequentially and coaxially arranged, and the optical fiber coupler is communicated with the monochromator through a multimode optical fiber; a dichroic mirror module is arranged between the objective lens and the total reflection mirror, a pulse laser is arranged on the other side face of the inverted optical microscope, an adjustable neutral filter plate group, a diaphragm and a periscope group are sequentially arranged in the output direction of the pulse laser, and laser with a periodic short pulse sequence output by the pulse laser sequentially passes through the adjustable neutral filter plate group, the diaphragm and the periscope group to reach the dichroic mirror module and be reflected to the objective lens, so that the laser is focused to a specific area of a sample.
In some embodiments of the present invention, an optical filter may be further disposed in the optical path between the beam expander and the optical fiber coupler.
In some embodiments of the present invention, a polarizer may be further disposed in the optical path between the beam expander and the fiber coupler.
Compared with the prior art, the utility model discloses there is following advantage:
the embodiment of the utility model provides a fluorescence emission spectrum combines together fluorescence life-span formation of image and fluorescence wavelength detection with life-span detection system, further follows the attribute of two kinds of different dimensions simultaneous study objects, surveys simultaneously through having fused two kinds of parameters of fluorescence life-span and fluorescence wavelength, has integrateed the high spatial resolution who confocal to and the accurate screening of monochromator to the wavelength, has extended the reference dimension for studying object light physics chemical properties, thereby has promoted the reliability and the comprehensive nature of experiment.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a front view of a fluorescence emission spectroscopy and lifetime detection system according to the present invention;
fig. 2 is a side view of the fluorescence emission spectrum and lifetime detection system of the present invention.
Description of reference numerals:
1. inverting the optical microscope; 2. a sample; 3. an objective lens; 4. a total reflection mirror; 5. a housing; 6. a focusing mirror; 7. the pinhole can be adjusted; 8. a beam expander; 9. a fiber coupler; 10. a multimode optical fiber; 11. a monochromator; 12. a single photon detector; 13. a dichroic mirror module; 14. a periscope group; 15. a diaphragm; 16. an adjustable neutral filter plate group; 17. a pulsed laser.
Detailed Description
In order to make the technical field person understand the scheme of the present invention better, the following will combine the drawings in the embodiments of the present invention to clearly and completely describe the technical scheme in the embodiments of the present invention. It is to be understood that the embodiments described are merely exemplary of the invention, and that no limitations are intended to the details of construction or design herein shown. The present invention may be embodied in many different forms and is not limited to the embodiments described herein, which are provided for the purpose of providing a more thorough understanding of the present disclosure.
Referring to fig. 1-2, the fluorescence emission spectrum and lifetime detection system includes an inverted optical microscope 1, an objective lens 3 is disposed under a sample stage 2 of the inverted optical microscope 1, and a sample 2 for fluorescence detection is fixedly disposed on the sample stage 2 corresponding to the objective lens 3; a total reflection mirror 4 is arranged right below the objective lens 3; a light-blocking shell 5 is arranged on one side of the inverted optical microscope 1, a monochromator 11 and a single-photon detector 12 are sequentially arranged on the outer side of the shell 5, a focusing lens 6, an adjustable pinhole 7, a beam expander 8 and an optical fiber coupler 9 are arranged in the shell 5, the total reflection lens 4, the focusing lens 6, the adjustable pinhole 7, the beam expander 8 and the optical fiber coupler 9 are sequentially and coaxially arranged, and the optical fiber coupler 9 is communicated with the monochromator 11 through a multimode optical fiber 10; a dichroic mirror module 13 is arranged between the objective lens 3 and the total reflection mirror 4, a pulse laser 17 is arranged on the other side surface of the inverted optical microscope 1, an adjustable neutral filter plate group 16, a diaphragm 15 and a periscope 14 are sequentially arranged in the output direction of the pulse laser 17, and laser with a periodic short pulse sequence output by the pulse laser 17 sequentially passes through the adjustable neutral filter plate group 16, the diaphragm 15 and the periscope 14 to reach the dichroic mirror module 13 and be reflected to the objective lens 3, so as to be focused on a specific area of the sample 2.
In the case of fluorescence excitation, using photoluminescence, as shown in fig. 1, the sample 2 is first placed on a suitable jig and then placed on a sample 2 stage, and since the excitation light source enters behind the inverted optical microscope 1, as shown in fig. 2, the pulse width of the laser light having a periodic short pulse sequence output from the pulse laser 17 is in the order of femtosecond of picoseconds or less. The light height is adjusted to the height of the first layer of light path of the inverted optical microscope 1 through a series of light path guiding of the adjustable neutral filter plate group 16, the diaphragm 15 and the periscope group 14, enters from the rear inlet of the first layer of light path of the inverted optical microscope 1, and then is reflected to the objective lens 3 through the dichroic mirror module 13, so that the laser is focused to a specific area of the sample 2, and the fluorescence life of the sample 2 emitting light can be measured by using time-dependent single photon counting (TCSPC).
In the aspect of fluorescence detection, fluorescence emitted by a sample 2 excited by electric energy passes through an objective lens 3, is output through a left outlet of a lower-layer light path of an inverted optical microscope 1, passes through a special detection unit, and comprises a focusing lens 6, an adjustable pinhole 7, a beam expander 8 and an optical fiber coupler 9 which are sequentially arranged in a light-isolating shell 5, and is collected into the optical fiber coupler 9, so that the fluorescence is transmitted to a monochromator 11 on the other side through a multimode optical fiber 10, the monochromator 11 is responsible for wavelength scanning and screening light, and the light subjected to wavelength screening is transmitted to a photosensitive surface of a single photon detector 12 through an outlet. Specifically, in order to improve the spatial resolution, a specific sample 2 region is screened out for observation by using the focusing function of the conventional inverted optical microscope 1. The embodiment of the utility model provides a set up the pinhole light path of adjustable pinhole 7, reached the spatial resolution who presses close to the diffraction limit, concrete light path principle is exactly to receive earlier through focusing mirror 6 and restraint the fluorescence on the detection light path, and the light beam after receiving passes through the pinhole of particular position, and this pinhole further filters fluorescent beam, reaches higher resolution ratio back, and another supporting beam expanding mirror 8 expands the beam again, and fluorescence changes back the parallel light. The parallel light finally enters the optical fiber coupler 9, is transmitted to a monochromator 11 system through an optical fiber, and is transmitted to the single-photon detector 12 through an outlet slit, so that the detection of the fluorescence with specific wavelength is completed. After the information is collected, fluorescence lifetime imaging information under each wavelength is obtained through data analysis.
The embodiment of the utility model provides an in, can also be provided with the light filter in the light path between beam expanding lens 8 and optical fiber coupler 9 for filter the light of different wavelengths.
The embodiment of the utility model provides an in, can also be provided with the polaroid in the light path between beam expanding mirror 8 and optical fiber coupler 9, because low temperature detector may have great polarization sensitivity, can be used to filter the fluorescence of specific polarization for the polarization characteristic that research sample 2 sent fluorescence.
In the embodiment of the present invention, the narrow band-pass filter can be added in the exit of the pulse laser 17, so as to further improve the monochromaticity of the laser.
The embodiment of the utility model provides a fluorescence emission spectrum combines together fluorescence life-span formation of image and fluorescence wavelength detection with life-span detection system, further follows the attribute of two kinds of different dimensions simultaneous study objects, surveys simultaneously through having fused two kinds of parameters of fluorescence life-span and fluorescence wavelength, has integrateed the high spatial resolution who focuses to and monochromator 11 is to the accurate screening of wavelength, has extended the reference dimension for studying object photophysical chemical properties, thereby has promoted the reliability and the comprehensive nature of experiment.
Those not described in detail in this specification are within the skill of the art. Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described in the foregoing detailed description, or equivalent replacements may be made for some of the technical features of the embodiments. All utilize the equivalent structure that the content of the utility model discloses a specification and attached drawing was done, direct or indirect application is in other relevant technical field, all is in the same way the utility model discloses within the patent protection scope.
Claims (4)
1. A fluorescence emission spectroscopy and lifetime detection system, comprising: the system comprises an inverted optical microscope, wherein an objective lens is arranged under a sample stage of the inverted optical microscope, and a sample for fluorescence detection is fixedly placed on the sample stage corresponding to the position of the objective lens; a total reflection mirror is arranged right below the objective lens; a light-blocking shell is arranged on one side of the inverted optical microscope, a monochromator and a single-photon detector are sequentially arranged on the outer side of the shell, a focusing lens, an adjustable pinhole, a beam expanding lens and an optical fiber coupler are arranged in the shell, the total reflection lens, the focusing lens, the adjustable pinhole, the beam expanding lens and the optical fiber coupler are sequentially and coaxially arranged, and the optical fiber coupler is communicated with the monochromator through a multimode optical fiber; a dichroic mirror module is arranged between the objective lens and the total reflection mirror, a pulse laser is arranged on the other side face of the inverted optical microscope, an adjustable neutral filter plate group, a diaphragm and a periscope group are sequentially arranged in the output direction of the pulse laser, and laser with a periodic short pulse sequence output by the pulse laser sequentially passes through the adjustable neutral filter plate group, the diaphragm and the periscope group to reach the dichroic mirror module and be reflected to the objective lens, so that the laser is focused to a specific area of a sample.
2. The fluorescence emission spectroscopy and lifetime detection system of claim 1, wherein: and an optical filter can be arranged in an optical path between the beam expander and the optical fiber coupler.
3. The fluorescence emission spectroscopy and lifetime detection system of claim 1, wherein: and a polaroid can be arranged in the light path between the beam expander and the optical fiber coupler.
4. The fluorescence emission spectroscopy and lifetime detection system of claim 1, wherein: and a narrow band-pass filter is additionally arranged at the outlet of the pulse laser.
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| CN202022985207.2U CN214097162U (en) | 2020-12-10 | 2020-12-10 | Fluorescence emission spectrum and service life detection system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114894758A (en) * | 2022-04-29 | 2022-08-12 | 南方科技大学 | Fluorescence analysis system and method |
| CN117129426A (en) * | 2023-08-08 | 2023-11-28 | 华东师范大学 | Ultrafast time-resolved circular polarization emission spectrometer |
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2020
- 2020-12-10 CN CN202022985207.2U patent/CN214097162U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114894758A (en) * | 2022-04-29 | 2022-08-12 | 南方科技大学 | Fluorescence analysis system and method |
| CN117129426A (en) * | 2023-08-08 | 2023-11-28 | 华东师范大学 | Ultrafast time-resolved circular polarization emission spectrometer |
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