CN213933595U - High-voltage fluorescence service life detection system - Google Patents
High-voltage fluorescence service life detection system Download PDFInfo
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- CN213933595U CN213933595U CN202022979256.5U CN202022979256U CN213933595U CN 213933595 U CN213933595 U CN 213933595U CN 202022979256 U CN202022979256 U CN 202022979256U CN 213933595 U CN213933595 U CN 213933595U
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Abstract
The utility model discloses a high pressure fluorescence life-span detection system that gives out light relates to fluorescence technical field, and this high pressure fluorescence life-span detection system that gives out light is including inverting optical microscope, sample, objective, total reflection mirror, shell, focusing mirror, adjustable pinhole, beam expanding mirror, optical detector, high-pressure diamond device, dichroscope module, periscope group, diaphragm, adjustable neutral filter group and pulse laser. The utility model discloses a photoluminescence fluorescence emission under the high pressure condition, the pressure of high strength is applyed in sample specified area, then carries out photoinduced excitation to this region again, then gathers the fluorescence that the sample sent to carry out data record and fitting analysis, and the value of pressure can be adjusted, through the value and the region of action of control pressure, realizes the quantitative research of accurate pressure and fluorescence life-span correlation.
Description
Technical Field
The utility model relates to a fluorescence technology field especially relates to a high pressure fluorescence life-span detection system that gives out light.
Background
The fluorescence emitted by the object contains various photophysical properties. For example, the uniformity of a single-molecule coating film, the photoelectric conversion and storage efficiency of a photovoltaic solar cell material, the energy transfer between molecules, the configuration of macromolecular protein, the material transfer characteristic in cells and the like are judged. Can be effectively quantified and calibrated by measuring the fluorescence lifetime of the fluorescent material. Fluorescence is used as light energy and is converted from other energy in the same or different forms, the common form is that the light energy is converted with each other, the sample is stimulated by the short-wavelength light energy with higher energy, and the sample emits the long-wavelength fluorescence with lower energy through energy level transition and internal energy conversion mechanisms, and the experimental mode is common at present. However, the current technical means for studying the fluorescence lifetime change of some pressure-sensitive materials or other samples by combining the high-pressure effect with the means of fluorescence excitation caused by common light energy stimulation is still in the exploration stage, so that the applicant provides a high-pressure fluorescence emission lifetime detection system to solve the problem.
SUMMERY OF THE UTILITY MODEL
In order to overcome the defects of related products in the prior art, the utility model provides a high-pressure fluorescence service life detection system.
The utility model provides a high pressure fluorescence life-span detection system that gives out light, include: the device comprises an inverted optical microscope, wherein an objective lens is arranged under a sample stage of the inverted optical microscope, a sample for fluorescence detection is fixedly placed on the sample stage corresponding to the position of the objective lens, and two ends of the sample are arranged in a high-pressure diamond device; a total reflection mirror is arranged right below the objective lens; a light-blocking shell is arranged on one side of the inverted optical microscope, a focusing lens, an adjustable pinhole, a beam expanding lens and an optical detector are arranged in the shell, and the total reflection lens, the focusing lens, the adjustable pinhole, the beam expanding lens and the optical detector are coaxially arranged in sequence; a dichroic mirror module is arranged between the objective lens and the total reflection mirror, a pulse laser is arranged on the other side face of the inverted optical microscope, an adjustable neutral filter plate group, a diaphragm and a periscope group are sequentially arranged in the output direction of the pulse laser, and laser with a periodic short pulse sequence output by the pulse laser sequentially passes through the adjustable neutral filter plate group, the diaphragm and the periscope group to reach the dichroic mirror module and be reflected to the objective lens, so that the laser is focused to a specific area of a sample.
In some embodiments of the present invention, an optical filter may be further disposed in the optical path between the beam expander and the optical detector.
In some embodiments of the present invention, a polarizer may be further disposed in the optical path between the beam expander and the optical detector.
In some embodiments of the present invention, a narrow band-pass filter is added at the outlet of the pulse laser.
Compared with the prior art, the utility model discloses there is following advantage:
the embodiment of the utility model provides a high pressure fluorescence is luminous life-span detection system is through the photoluminescence emission under the high pressure condition, exerts the pressure of high strength in sample specified area, then carries out photoinduced excitation to this region again, then gathers the fluorescence that the sample sent to carry out data record and fitting analysis, and the value of pressure can be adjusted, and value and the region of action through control pressure realize the quantitative research of accurate pressure and fluorescence life-span correlation.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a front view of the high-pressure fluorescence lifetime detection system of the present invention;
fig. 2 is a side view of the high-pressure fluorescence lifetime detection system of the present invention.
Description of reference numerals:
1. inverting the optical microscope; 2. a sample; 3. an objective lens; 4. a total reflection mirror; 5. a housing; 6. a focusing mirror; 7. the pinhole can be adjusted; 8. a beam expander; 9. an optical detector; 10. a high pressure diamond device; 11. a dichroic mirror module; 12. a periscope group; 13. a diaphragm; 14. an adjustable neutral filter plate group; 15. a pulsed laser.
Detailed Description
In order to make the technical field person understand the scheme of the present invention better, the following will combine the drawings in the embodiments of the present invention to clearly and completely describe the technical scheme in the embodiments of the present invention. It is to be understood that the embodiments described are merely exemplary of the invention, and that no limitations are intended to the details of construction or design herein shown. The present invention may be embodied in many different forms and is not limited to the embodiments described herein, which are provided for the purpose of providing a more thorough understanding of the present disclosure.
Referring to fig. 1-2, the high-voltage fluorescence lifetime detection system comprises an inverted optical microscope 1, an objective lens 3 is arranged under a sample 2 stage of the inverted optical microscope 1, a fluorescence detection sample 2 is fixedly arranged on the sample 2 stage corresponding to the objective lens 3, and two ends of the sample 2 are arranged in a high-voltage diamond device 10; a total reflection mirror 4 is arranged right below the objective lens 3; a light-blocking shell 5 is arranged on one side of the inverted optical microscope 1, a focusing lens 6, an adjustable pinhole 7, a beam expanding lens 8 and an optical detector 9 are arranged in the shell 5, and the total reflection lens 4, the focusing lens 6, the adjustable pinhole 7, the beam expanding lens 8 and the optical detector 9 are coaxially arranged in sequence; a dichroic mirror module 11 is arranged between the objective lens 3 and the total reflection mirror 4, a pulse laser 15 is arranged on the other side surface of the inverted optical microscope 1, an adjustable neutral filter plate group 14, a diaphragm 13 and a periscope group 12 are sequentially arranged in the output direction of the pulse laser 15, and laser with a periodic short pulse sequence output by the pulse laser 15 sequentially passes through the adjustable neutral filter plate group 14, the diaphragm 13 and the periscope group 12 to reach the dichroic mirror module 11 and be reflected to the objective lens 3, so that the laser is focused to a specific area of the sample 2.
In fluorescence excitation, using electroluminescence, as shown in fig. 1, a sample 2 is first placed in a diamond high-pressure device and then placed on a stage of the sample 2, and since the diamond high-pressure device significantly increases the distance between the sample 2 and the objective lens 3, the objective lens 3 needs to use a long-focus objective lens 3 to focus light onto the sample 2. Since the excitation light source enters behind the inverted optical microscope 1, as shown in fig. 2, the pulse width of the laser light having a periodic short pulse sequence output from the pulse laser 15 is in the order of femtosecond picoseconds or less. The light height is adjusted to the height of the first layer of light path of the inverted optical microscope 1 through a series of light path guiding of the adjustable neutral filter group 14, the diaphragm 13 and the periscope group 12, enters from the rear inlet of the first layer of light path of the inverted optical microscope 1, and then is reflected to the objective lens 3 through the dichroic mirror module 11, so that the laser is focused to a specific area of the sample 2, and the fluorescence life of the sample 2 emitting light can be measured by using time-dependent single photon counting (TCSPC).
In the aspect of fluorescence detection, fluorescence emitted by a sample 2 excited by electric energy passes through an objective lens 3, is output through a left outlet of a lower-layer light path of an inverted optical microscope 1, and passes through a special detection unit comprising a focusing lens 6, an adjustable pinhole 7, a beam expander 8 and an optical detector 9 which are sequentially arranged in a light-isolating shell 5, and the fluorescence is collected into the optical detector 9, wherein the optical detector 9 is a single-photon detector. Specifically, in order to improve the spatial resolution, a specific sample 2 region is screened out for observation by using the focusing function of the conventional inverted optical microscope 1. The embodiment of the utility model provides a set up the pinhole light path of adjustable pinhole 7, reached the spatial resolution who presses close to the diffraction limit, concrete light path principle is exactly to receive earlier through focusing mirror 6 and restraint the fluorescence on the detection light path, and the light beam after receiving passes through the pinhole of particular position, and this pinhole further filters fluorescent beam, reaches higher resolution ratio back, and another supporting beam expanding mirror 8 expands the beam again, and fluorescence changes back the parallel light. The parallel light is finally incident on the photosensitive surface of the optical detector 9, and the detection of the fluorescence is completed.
The embodiment of the utility model provides an in, can also be provided with the light filter in the light path between beam expanding lens 8 and the optical detector 9 for filter the light of different wavelengths.
The embodiment of the utility model provides an in, can also be provided with the polaroid in the light path between beam expanding lens 8 and optical detector 9, because low temperature detector can have great polarization sensitivity, can be used to filter the fluorescence of specific polarization for the polarization characteristic that research sample 2 sent fluorescence.
In the embodiment of the present invention, the narrow band-pass filter can be added in the exit of the pulse laser 15, so as to further improve the monochromaticity of the laser.
The embodiment of the utility model provides a high pressure fluorescence light-emitting life detection system utilizes the high pressure effect to combine together with the means that common light energy stimulation leads to fluorescence excitation, come some fluorescence life-span change to pressure sensitive material or other sample 2 of further research, photoluminescence emission through under the high-pressure condition, exert the pressure of high strength in sample 2 specified area, then carry out photoluminescence to this region again, then gather the fluorescence that sample 2 sent, and carry out data record and fitting analysis, and the value of pressure can be adjusted, value and the region of action through control pressure, realize accurate pressure and the quantitative research of fluorescence life-span correlation.
Those not described in detail in this specification are within the skill of the art. Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described in the foregoing detailed description, or equivalent replacements may be made for some of the technical features of the embodiments. All utilize the equivalent structure that the content of the utility model discloses a specification and attached drawing was done, direct or indirect application is in other relevant technical field, all is in the same way the utility model discloses within the patent protection scope.
Claims (4)
1. A high-voltage fluorescence lifetime detection system, comprising: the device comprises an inverted optical microscope, wherein an objective lens is arranged under a sample stage of the inverted optical microscope, a sample for fluorescence detection is fixedly placed on the sample stage corresponding to the position of the objective lens, and two ends of the sample are arranged in a high-pressure diamond device; a total reflection mirror is arranged right below the objective lens; a light-blocking shell is arranged on one side of the inverted optical microscope, a focusing lens, an adjustable pinhole, a beam expanding lens and an optical detector are arranged in the shell, and the total reflection lens, the focusing lens, the adjustable pinhole, the beam expanding lens and the optical detector are coaxially arranged in sequence; a dichroic mirror module is arranged between the objective lens and the total reflection mirror, a pulse laser is arranged on the other side face of the inverted optical microscope, an adjustable neutral filter plate group, a diaphragm and a periscope group are sequentially arranged in the output direction of the pulse laser, and laser with a periodic short pulse sequence output by the pulse laser sequentially passes through the adjustable neutral filter plate group, the diaphragm and the periscope group to reach the dichroic mirror module and be reflected to the objective lens, so that the laser is focused to a specific area of a sample.
2. The high-voltage fluorescence lifetime detection system according to claim 1, wherein: an optical filter can be arranged in an optical path between the beam expanding lens and the optical detector.
3. The high-voltage fluorescence lifetime detection system according to claim 1, wherein: and a polaroid can be arranged in the light path between the beam expander and the optical detector.
4. The high-voltage fluorescence lifetime detection system according to claim 1, wherein: and a narrow band-pass filter is additionally arranged at the outlet of the pulse laser.
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| CN202022979256.5U CN213933595U (en) | 2020-12-10 | 2020-12-10 | High-voltage fluorescence service life detection system |
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| CN202022979256.5U CN213933595U (en) | 2020-12-10 | 2020-12-10 | High-voltage fluorescence service life detection system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114234863A (en) * | 2022-02-23 | 2022-03-25 | 三代光学科技（天津）有限公司 | High-precision measuring light path structure for surface roughness of inner cavity and outer cavity and automatic measuring system |
| CN114894758A (en) * | 2022-04-29 | 2022-08-12 | 南方科技大学 | Fluorescence analysis system and method |
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2020
- 2020-12-10 CN CN202022979256.5U patent/CN213933595U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114234863A (en) * | 2022-02-23 | 2022-03-25 | 三代光学科技（天津）有限公司 | High-precision measuring light path structure for surface roughness of inner cavity and outer cavity and automatic measuring system |
| CN114894758A (en) * | 2022-04-29 | 2022-08-12 | 南方科技大学 | Fluorescence analysis system and method |
| CN114894758B (en) * | 2022-04-29 | 2024-10-01 | 南方科技大学 | Fluorescence analysis system and method |
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