CN111149773B - Drosophila resistance strain screening system - Google Patents
Drosophila resistance strain screening system Download PDFInfo
- Publication number
- CN111149773B CN111149773B CN202010097736.0A CN202010097736A CN111149773B CN 111149773 B CN111149773 B CN 111149773B CN 202010097736 A CN202010097736 A CN 202010097736A CN 111149773 B CN111149773 B CN 111149773B
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- drosophila
- tube
- pipe
- fruit bat
- director
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- 241000255581 Drosophila <fruit fly, genus> Species 0.000 title claims abstract description 28
- 238000012216 screening Methods 0.000 title claims abstract description 23
- 239000000463 material Substances 0.000 claims abstract description 8
- 235000013399 edible fruits Nutrition 0.000 claims abstract 18
- 239000001963 growth medium Substances 0.000 claims description 9
- 239000000575 pesticide Substances 0.000 claims description 7
- 241000238631 Hexapoda Species 0.000 abstract description 5
- 206010059866 Drug resistance Diseases 0.000 abstract description 3
- 241000255588 Tephritidae Species 0.000 description 84
- 241000255601 Drosophila melanogaster Species 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000029264 phototaxis Effects 0.000 description 2
- 230000003542 behavioural effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/0333—Genetically modified invertebrates, e.g. transgenic, polyploid
- A01K67/0337—Genetically modified Arthropods
- A01K67/0339—Genetically modified insects, e.g. Drosophila melanogaster, medfly
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention provides a drosophila resistance strain screening system, belongs to the field of insect research, and provides a system capable of screening drosophila with drug resistance. Including a plurality of fruit bat cultivation pipe, each fruit bat cultivation pipe all disposes the stopcock, the stopcock lower part is connected with fruit bat cultivation pipe upper portion, last stopcock upper portion is connected with the light pipe front end, the light pipe is terminal to be connected with next fruit bat cultivation pipe upper portion, fruit bat cultivation pipe lateral wall upper portion except that first fruit bat cultivation pipe all is equipped with yellow green light source, light pipe internally mounted has the fruit bat director, the light pipe rear end is connected with the fruit bat director, the fruit bat director is the round platform shape and is one-way leaded light material, the diameter of the first disc of fruit bat director is greater than the diameter of second disc, the first disc of the fruit bat director of light pipe inside is located before its second disc, the cylindrical opening of the cylindrical pipe that yellow green light source is located just to the second disc of the fruit bat director that the light pipe end is connected, each yellow green light source all is connected with controlling means and power.
Description
Technical Field
The invention relates to the technical field of insect research, in particular to a drosophila resistant strain screening system.
Background
Screening for resistant lines is important for entomology research. Fruit flies are an important model insect with many advantages not found in non-model insects. The directed screening of the strain of the drosophila melanogaster which can resist a certain pesticide or chemical agent is particularly important for the deep research of the detoxification mechanism and the metabolic mechanism of the drosophila melanogaster. For this reason, it is necessary to provide a system capable of performing line screening for resistance to Drosophila.
Disclosure of Invention
The invention aims to provide a drosophila resistance strain screening system capable of screening drosophila with certain drug resistance.
In order to solve the technical problems, the invention adopts the technical scheme that:
a fruit fly resistant strain screening system comprises a plurality of fruit fly culture tubes, wherein a culture medium is placed into each fruit fly culture tube which is sequentially arranged from left to right, a pesticide is placed into the culture medium, the pesticide amount placed into each fruit fly culture tube which is sequentially arranged from left to right is gradually increased, the fruit fly culture tubes are cylindrical, each fruit fly culture tube is provided with a tube plug which is in an inverted funnel shape, the lower part of the tube plug is connected with the upper part of the fruit fly culture tube, the upper part of the previous tube plug is connected with the front end of a light guide tube, the tail end of the light guide tube is connected with the upper part of the next fruit fly culture tube, yellow green light sources are arranged on the upper parts of the side walls of other fruit fly culture tubes except the first fruit fly culture tube, the yellow green light sources are arranged in the cylindrical tube with an opening at one end, a plurality of fruit fly guides are arranged in the light guide tube, and the tail end of the light guide tube is also connected with a fruit fly guide, the fruit fly guider is in a round table shape and is made of a one-way light guide material, the diameter of a first round surface of the fruit fly guider is larger than that of a second round surface, the first round surface of the fruit fly guider in the light guide pipe is positioned in front of the second round surface, an opening of a cylindrical pipe where a yellow-green light source is located is over against the second round surface of the fruit fly guider connected with the tail end of the light guide pipe, and each yellow-green light source is connected with the control device and the power supply.
Optionally, the light pipe is provided with an electromagnetic valve, and the electromagnetic valve is connected with the control device and the power supply.
Optionally, the diameter of the second circular face of the drosophila guide is 3-4 mm.
Optionally, the lower part of the pipe plug is in threaded connection with the upper part of the fruit fly culture pipe.
Optionally, the electromagnetic valve is disposed at a connection of the plug and the light pipe.
Optionally, the plug is made of a gas permeable material.
The invention has the beneficial effects that:
through setting up structures such as fruit fly culture tube, stopcock, light pipe, yellow green light source, fruit fly director and controlling means, provide one kind and can carry out the system that sieves to the resistant strain of fruit fly, ensure through this system that the fruit fly strain of finally surviving is the resistant strain that passes through multistage screening survival.
Drawings
Fig. 1 is a schematic structural view of the present invention.
Fig. 2 is a schematic diagram of the circuit connection relationship according to the present invention.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and examples.
As shown in fig. 1 and fig. 2, the system for screening fruit fly resistant strains in the present embodiment includes a plurality of fruit fly culture tubes 1, a culture medium is placed in each fruit fly culture tube 1 arranged in sequence from left to right, a chemical is placed in the culture medium, the amount of the chemical placed in each fruit fly culture tube 1 arranged in sequence from left to right is gradually increased, the fruit fly culture tubes 1 are cylindrical, each fruit fly culture tube 1 is provided with a tube plug 2, the tube plug 2 is in an inverted funnel shape, the lower portion of the tube plug 2 is connected to the upper portion of the fruit fly culture tube 1, the upper portion of the previous tube plug 2 is connected to the front end of a light guide tube 3, the end of the light guide tube 3 is connected to the upper portion of the next fruit fly culture tube 1, the upper portions of the side walls of the other fruit fly culture tubes 1 except for the first fruit fly culture tube 1 are provided with yellow-green light sources 4, the yellow-green light sources 4 are placed in the cylindrical tube with an opening at one end, a plurality of fruit fly guides 5 are installed inside the light guide tube 3, the tail end of the light guide pipe 3 is also connected with a fruit fly guider 5, the fruit fly guider 5 is in a round table shape and is made of a one-way light guide material, the diameter of a first round surface of the fruit fly guider 5 is larger than that of a second round surface, the first round surface of the fruit fly guider 5 inside the light guide pipe 3 is positioned in front of the second round surface, an opening of a cylindrical pipe where the yellow-green light source 4 is located is over against the second round surface of the fruit fly guider 5 connected with the tail end of the light guide pipe 3, and each yellow-green light source 4 is connected with the control device 6 and the power supply 7. In fig. 1, the present invention is exemplified by including three drosophila culture tubes 1, and in the specific implementation, the number of the drosophila culture tubes 1 can be set according to the requirement.
Wherein the power supply 7 is used for supplying power to each yellow-green light source 4. The control device 6 is used for controlling the turn-on time and the turn-on duration of each yellow-green light source 4.
Optionally, the light pipe 3 is provided with an electromagnetic valve 8, and the electromagnetic valve 8 is connected with both the control device 6 and the power supply 7. The electromagnetic valve 8 is used for controlling whether to lead the fruit flies to the next fruit fly culture tube 1.
Optionally, the diameter of the second round surface of the fruit fly guide 5 is 3-4mm, so that 2-4 fruit flies can pass through the second round surface of the fruit fly guide 5 at the same time.
Optionally, the lower part of the pipe plug 2 is in threaded connection with the upper part of the fruit fly culture pipe 1.
Optionally, a solenoid valve 8 is provided at the junction of the plug 2 and the light pipe 3.
Wherein, the pipe plug 2 is made of a breathable material to ensure the normal breathing of the fruit flies.
When the fruit fly culture tube is used, a culture medium is firstly put into each fruit fly culture tube 1 which is sequentially arranged from left to right, a pesticide is put into the culture medium, the pesticide amount put into each fruit fly culture tube 1 which is sequentially arranged from left to right is gradually increased, and then the first generation fruit flies are put into the first fruit fly culture tube 1 for culture. After the first generation of fruit flies are cultured for a period of time and fruit flies with dosage capable of resisting the medicine in the first fruit fly culture tube 1 are left, the control device 6 controls the yellow-green light source 4 on the second fruit fly culture tube 1 to be turned on, and because the fruit flies have phototaxis (yellow-green light) and behavior preference tending to move upwards, the left fruit flies can move into the second fruit fly culture tube 1 through the light guide tube 3. Since the fruit flies have a preference to move from the wide surface to the narrow surface, by setting the diameter of the first circular surface of the fruit fly guide 5 to be larger than the diameter of the second circular surface, the fruit flies can be guided into the latter fruit fly culture tube 1 by the fruit fly guide 5 without generally moving in the opposite direction, so that the fruit flies generally tend to move in one direction. Since the fruit fly guide 5 is made of the unidirectional light guide material, the brightness of the second round surface of the fruit fly guide 5 is higher than that of the first round surface when viewed by fruit flies, and most of the fruit flies tend to enter the next fruit fly culture tube 1 through the second round surface of the fruit fly guide 5. And analogizing in turn until the drosophila melanogaster strain meeting certain drug resistance is screened out. When the electromagnetic valve 8 is arranged on the light guide pipe 3, if the fruit flies are required to be guided into the next fruit fly culture pipe 1, the control device 6 controls the electromagnetic valve 8 to be opened. The electromagnetic valve 8 is arranged at the joint of the pipe plug 2 and the light guide pipe 3, so that after the next fruit fly culture pipe 1 is led in the fruit flies and the electromagnetic valve 8 is closed, part of the fruit flies are prevented from being detained between the pipe plug 2 and the electromagnetic valve, and the part of the fruit flies are prevented from dying. The yellow-green light source 4 is arranged in the cylindrical tube with an opening at one end, the opening of the cylindrical tube with the yellow-green light source 4 is opposite to the second round surface of the fruit fly guider 5 connected with the tail end of the light guide tube 3, and light emitted by the yellow-green light source 4 can be directly irradiated into the second round surface of the fruit fly guider 5.
The method is based on phototaxis and movement behavior preference of the fruit fly imagoes, so that the fruit flies surviving after screening can be automatically transferred to the next fruit fly culture tube 1. Through multiple rounds of screening, the fruit fly survived in the culture medium with the highest concentration of the medicament is the resistant fruit fly which can tolerate the medicament, so that the invention can ensure that the finally remained fruit fly strain is the resistant strain survived through multiple rounds of screening. Of course, the invention can also be used for screening other insect strains with the same behavioral preference as fruit flies.
It will be understood that the above embodiments are merely exemplary embodiments taken to illustrate the principles of the present invention, which is not limited thereto. It will be apparent to those skilled in the art that various modifications and improvements can be made without departing from the spirit and substance of the invention, and these modifications and improvements are also considered to be within the scope of the invention.
Claims (6)
1. A drosophila resistant strain screening system is characterized by comprising a plurality of drosophila culture tubes (1), wherein a culture medium is placed in each drosophila culture tube (1) which is sequentially arranged from left to right, a pesticide is placed in the culture medium, the amount of the pesticide placed in each drosophila culture tube (1) which is sequentially arranged from left to right is gradually increased, the drosophila culture tubes (1) are cylindrical, each drosophila culture tube (1) is provided with a tube plug (2), the tube plug (2) is in an inverted funnel shape, the lower part of the tube plug (2) is connected with the upper part of the drosophila culture tube (1), the upper part of the last tube plug (2) is connected with the front end of the light guide tube (3), the tail end of the light guide tube (3) is connected with the upper part of the next drosophila culture tube (1), the upper parts of the side walls of other drosophila culture tubes (1) except the first drosophila culture tube (1) are provided with yellow-green light sources (4), and place in yellow green light source (4) in one end open-ended cylindrical tube, light pipe (3) internally mounted has a plurality of fruit bat director (5), the end of light pipe (3) also is connected with a fruit bat director (5), fruit bat director (5) are round platform shape and adopt one-way leaded light material to make, the diameter of the first disc of fruit bat director (5) is greater than the diameter of second disc, the first disc of the inside fruit bat director (5) of light pipe (3) is located before its second disc, the opening of the cylindrical tube that yellow green light source (4) were located is just to the second disc of the fruit bat director (5) that light pipe (3) end was connected, each yellow green light source (4) all are connected with controlling means (6) and power (7).
2. The drosophila-resistant strain screening system according to claim 1, wherein the light pipe (3) is provided with an electromagnetic valve (8), and the electromagnetic valve (8) is connected with both the control device (6) and the power supply (7).
3. The drosophila-resistant strain screening system according to claim 1, wherein the diameter of the second circular face of the drosophila guide (5) is 3-4 mm.
4. The drosophila-resistant strain screening system according to claim 1, wherein the lower part of the pipe plug (2) is screwed with the upper part of the drosophila culture pipe (1).
5. The drosophila-resistant strain screening system according to claim 2, wherein the solenoid valve (8) is arranged at the junction of the pipe plug (2) and the light pipe (3).
6. The drosophila-resistant strain screening system according to claim 1, wherein the plug (2) is made of a gas-permeable material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010097736.0A CN111149773B (en) | 2020-02-17 | 2020-02-17 | Drosophila resistance strain screening system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010097736.0A CN111149773B (en) | 2020-02-17 | 2020-02-17 | Drosophila resistance strain screening system |
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| Publication Number | Publication Date |
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| CN111149773A CN111149773A (en) | 2020-05-15 |
| CN111149773B true CN111149773B (en) | 2021-11-19 |
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| CN202010097736.0A Active CN111149773B (en) | 2020-02-17 | 2020-02-17 | Drosophila resistance strain screening system |
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| CN111149773A (en) | 2020-05-15 |
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