CN109566544A - A kind of drosophila culture collection device - Google Patents
A kind of drosophila culture collection device Download PDFInfo
- Publication number
- CN109566544A CN109566544A CN201910080006.7A CN201910080006A CN109566544A CN 109566544 A CN109566544 A CN 109566544A CN 201910080006 A CN201910080006 A CN 201910080006A CN 109566544 A CN109566544 A CN 109566544A
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- China
- Prior art keywords
- drosophila
- bottle
- collecting pipe
- collection device
- spherical
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000255581 Drosophila <fruit fly, genus> Species 0.000 title claims abstract description 87
- 206010002091 Anaesthesia Diseases 0.000 claims abstract description 38
- 230000037005 anaesthesia Effects 0.000 claims abstract description 38
- 230000035699 permeability Effects 0.000 claims abstract description 10
- 239000001963 growth medium Substances 0.000 claims abstract description 7
- 229920000742 Cotton Polymers 0.000 claims description 12
- 210000004027 cell Anatomy 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 5
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 4
- 238000011160 research Methods 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- 241000255925 Diptera Species 0.000 description 2
- 210000000349 chromosome Anatomy 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 108700002304 Drosophila can Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000255588 Tephritidae Species 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of drosophilas to cultivate collection device, including drosophila culture bottle, communicating pipe, spherical collecting pipe and anesthesia bottle；Culture medium is filled in the drosophila culture bottle, the bottle mouth position of the drosophila culture bottle is provided with vapor-permeable plug, connection mouth is additionally provided on the body of the drosophila culture bottle, and the connection mouth is connected to drosophila culture bottle；One end of the communicating pipe is socketed in the connection mouth, and the other end of communicating pipe is socketed on spherical collecting pipe, is provided with valve the communicating pipe between its ends between, and spherical collecting pipe is provided with permeability cell far from the side of communicating pipe；The anesthesia bottle is set to the lower section of spherical collecting pipe, and the bottleneck of anesthesia bottle is connected to the bottom of spherical collecting pipe.A kind of drosophila provided by the invention cultivates collection device, does not have to pat finger pipe when collecting drosophila, not will cause drosophila death, collecting will not fly out into the drosophila in anesthesia bottle, escape.
Description
Technical field
The present invention relates to drosophila culture technique fields, and in particular to a kind of drosophila culture collection device.
Background technique
Genetics experiments are most important links in genetics teaching, it is therefore intended that allow student by do it yourself to test come
The basic theory of science of heredity is verified, practice genetics experiments technology and analysis genetics experiments are as a result, in the hope of sharpening understanding in turn
The theoretical content of science of heredity is grasped, the setting of experimental project surrounds this purpose and is unfolded.From Morgan in 1909 by drosophila
As after the material of research hereditary variation and chromosome relationship, drosophila is just with its history of life is short, raising is easy, chromosome letter
Singly, the advantages that mutant is more become one of most important model invertebrate organism in life science, more and more experiments
Scientific research is carried out by model animal of drosophila in room, therefore, mass rearing drosophila and must continue conservation for a long time, drosophila in the laboratory in
The quality of raising is directly related to the quality of Genetics Experimental Teaching.In the prior art, the culture of drosophila is to be stamped tampon
Refer to and cultivated in pipe, the collection of drosophila is carried out with anesthesia bottle.When transfer refers to the drosophila in pipe, needs to pat finger pipe, drosophila is made to shake
The bottom for referring to pipe is fallen on, at this time if referring to that culture medium is thinning in pipe, the drosophila fallen that shakes can be stuck wing, and transfer does not come out, fruit
Fly can be dead；If culture medium prepare it is bad some are dry, have crack between culture medium meeting and bottle wall, drosophila once falls into,
It will lead to drosophila death.At the end of collection, anesthesia bottle is separated with pipe is referred to, respectively lid tampon, separated moment holds makes very much
It escapes and flies away at drosophila, it is many inconvenient and worried that these problems are taken to the research of drosophila related experiment, needs to improve.
Summary of the invention
For the defects in the prior art, the present invention provides a kind of drosophila culture collection device, does not have to when collecting drosophila
Finger pipe is patted, not will cause drosophila death, collecting will not fly out into the drosophila in anesthesia bottle, escape.
The present invention is achieved by the following technical solutions.
A kind of drosophila culture collection device, including drosophila culture bottle, communicating pipe, spherical collecting pipe and anesthesia bottle；
Culture medium is filled in the drosophila culture bottle, the bottle mouth position of the drosophila culture bottle is provided with vapor-permeable plug, institute
It states and is additionally provided with connection mouth on the body of drosophila culture bottle, the connection mouth is connected to drosophila culture bottle；
One end of the communicating pipe is socketed in the connection mouth, and the other end of communicating pipe is socketed on spherical collecting pipe,
It is provided with valve the communicating pipe between its ends between, spherical collecting pipe is provided with permeability cell far from the side of communicating pipe；
The anesthesia bottle is set to the lower section of spherical collecting pipe, and the bottleneck of anesthesia bottle is connected to the bottom of spherical collecting pipe.
As a further improvement of the present invention, the bottle mouth position of the anesthesia bottle is provided with bottle cap, is provided on the bottle cap
Through-hole, which, which is configured with, can fill in the stopple of the through-hole, and the centre of the stopple is provided with cotton hole, in the cotton hole
Filled with cotton.
As a further improvement of the present invention, strainer is provided in the permeability cell, the aperture of the strainer is no more than
0.5mm。
As a further improvement of the present invention, the other end of the permeability cell is connected with asepwirator pump.
As a further improvement of the present invention, the vapor-permeable plug is rubber stopper, offers air hole on the rubber stopper,
The aperture of the air hole is no more than 0.5mm.
As a further improvement of the present invention, moth-killing device is provided on the outside of the spherical collecting pipe.
As a further improvement of the present invention, the moth-killing device be arranged on the outside of the spherical collecting pipe with it is described
The contour trap lamp of spherical collecting pipe.
As a further improvement of the present invention, the moth-killing device is to be pasted onto shining on the outside of the spherical collecting pipe
Diode.
As a further improvement of the present invention, it is provided with funnel between the spherical collecting pipe and the anesthesia bottle, it is described
The lower end of funnel is inserted into the bottleneck of the anesthesia bottle, and the upper end of the funnel is connected to the bottom of the spherical collecting pipe.
The beneficial effects of the present invention are embodied in:
A kind of drosophila provided by the invention cultivates collection device, and manufacturing cost is low, can promote the use of on a large scale, work as needs
When collecting drosophila, by connection pipe sleeve in connection mouth, and valve is opened, the drosophila in drosophila culture bottle will be toward air circulation
Spherical collecting pipe direction is flown to, and when the drosophila aggregation in spherical collecting pipe is excessive, gently touches spherical collecting pipe fruit at this time
Fly will just fall into anesthesia bottle, when having shifted, first close the valve of communicating pipe, all turn to the drosophila in spherical collecting pipe
When being moved into anesthesia bottle, spherical collecting pipe and anesthesia bottle are separated, moment covers the stopple on anesthesia bottle, in the cotton of hole beyond the Great Wall
Spend middle dropwise addition ether, so that it may smoothly drosophila be anaesthetized, finally separate spherical collecting pipe and culture bottle.
Detailed description of the invention
It, below will be to specific in order to illustrate more clearly of the specific embodiment of the invention or technical solution in the prior art
Embodiment or attached drawing needed to be used in the description of the prior art are briefly described.In all the appended drawings, similar element
Or part is generally identified by similar appended drawing reference.In attached drawing, each element or part might not be drawn according to actual ratio.
Fig. 1 is a kind of drosophila culture collection device structural schematic diagram in the embodiment of the present invention.
Specific embodiment
It is described in detail below in conjunction with embodiment of the attached drawing to technical solution of the present invention.Following embodiment is only used for
Clearly illustrate technical solution of the present invention, therefore be only used as example, and cannot be used as a limitation and limit protection model of the invention
It encloses.
It should be noted that unless otherwise indicated, technical term or scientific term used in this application should be this hair
The ordinary meaning that bright one of ordinary skill in the art are understood.
Embodiment
As shown in Figure 1, a kind of drosophila cultivates collection device, including drosophila culture bottle 1, communicating pipe 5,7 and of spherical collecting pipe
Anesthesia bottle 8.
Drosophila culture bottle 1 is for filling culture medium 2.The bottle mouth position of drosophila culture bottle 1 is provided with vapor-permeable plug 3, this implementation
The vapor-permeable plug 3 of example can be rubber stopper, and air hole is offered on rubber stopper 3, and the aperture of air hole is no more than 0.5mm, breathes freely
Rubber stopper 3 can guarantee that there is in drosophila culture bottle 1 sufficient oxygen, normally live for drosophila.Certainly, vapor-permeable plug 3
But not limited to this, in other embodiments, vapor-permeable plug 3 may be replaced by ventilative cotton plug etc., as long as being able to achieve ventilative
And prevent drosophila from flying out.Connection mouth 4, connection mouth 4 and drosophila culture bottle 1 are additionally provided on the body of drosophila culture bottle 1
Inner cavity connection.
In the present embodiment, communicating pipe 5 is rubber tube, but not limited to this.One end of communicating pipe 5 is socketed in connection mouth 4,
The other end of communicating pipe 5 is socketed on spherical collecting pipe 7.Communicating pipe 5 is provided with valve 6 between its ends between.Spherical collecting pipe 7
Side far from communicating pipe 5 is provided with permeability cell 10.When valve 6 is in close state, the fruit in drosophila culture bottle 1 can be prevented
Fly is mobile to spherical 7 direction of collecting pipe；On the contrary, drosophila in drosophila culture bottle 1 being capable of edge when valve 6 is in the open state
It is moved to spherical collecting pipe 7 communicating pipe 5.
The bottom end of spherical collecting pipe 7 is connected with anesthesia bottle 8, and anesthesia bottle 8 is set to the lower section of spherical collecting pipe 7.Anesthesia bottle 8
Bottleneck is connected to the bottom of spherical collecting pipe 7.The bottle mouth position of anesthesia bottle 8 is provided with bottle cap, is provided with through-hole on bottle cap, the anesthesia
For bottle 8 configured with the stopple 9 that can fill in through-hole, the centre of stopple 9 is provided with cotton hole, is filled with cotton in cotton hole.The present embodiment
Stopple 9 can be hung on bottle cap by a flexible strand.
Strainer 11 is provided in permeability cell 10, the aperture of strainer 11 is no more than 0.5mm.In the present embodiment of the invention,
The outside of spherical collecting pipe 7 be pasted with can light emitting diode 12, in another embodiment, can light emitting diode 12 can replace
For the trap lamp contour with spherical collecting pipe 7.Can light emitting diode 12 or trap lamp, drosophila can be lured from drosophila culture bottle
1 is mobile to the direction of spherical collecting pipe 7, therefore can promote the efficiency of drosophila collection.
In the present embodiment of the invention, it is provided with funnel 13 between spherical collecting pipe 7 and anesthesia bottle 8, under funnel 13
In the bottleneck of end insertion anesthesia bottle 8, the upper end of funnel 13 is connected to the bottom of spherical collecting pipe 7.The setting of funnel 13, Ke Yifang
Just drosophila falls into anesthesia bottle 8, simultaneously because the tip of funnel 13 is downward, can effectively prevent drosophila out of anesthesia bottle 8 again
It flies in spherical collecting pipe 7.
In the present embodiment of the invention, the other end of permeability cell 10 is connected with asepwirator pump 14, and the setting of asepwirator pump 14 can
To promote the flowing velocity of air in spherical collecting pipe 7, drosophila is mobile along air-flow, and then improves the transfer velocity of drosophila.
The present embodiment drosophila culture collection device in use, first by the both ends of communicating pipe 5 respectively with drosophila culture bottle 1
It is connected with spherical collecting pipe 7, spherical collecting pipe 7 is connected with anesthesia bottle 8, when needing to collect drosophila, and opens valve, drosophila training
Supporting the drosophila in bottle 1 will fly to toward 7 direction of spherical collecting pipe of air circulation, when the drosophila in spherical collecting pipe 7 assembled
When more, gently touching spherical 7 drosophila of collecting pipe at this time will just be fallen into anesthesia bottle 8, when having shifted, first close communicating pipe
5 valve 6 divides spherical collecting pipe 7 with anesthesia bottle 8 when the drosophila in spherical collecting pipe 7 is all transferred into anesthesia bottle 8
It opens, moment covers the stopple 9 on anesthesia bottle 8, ether is added dropwise in the cotton on stopple 9, so that it may smoothly anaesthetize drosophila, most
Spherical collecting pipe 7 and culture bottle 1 are separated afterwards.
Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention., rather than its limitations；To the greatest extent
Pipe present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that: its according to
So be possible to modify the technical solutions described in the foregoing embodiments, or to some or all of the technical features into
Row equivalent replacement；And these are modified or replaceed, various embodiments of the present invention technology that it does not separate the essence of the corresponding technical solution
The range of scheme should all cover within the scope of the claims and the description of the invention.
Claims (9)
1. a kind of drosophila cultivates collection device, which is characterized in that including drosophila culture bottle, communicating pipe, spherical collecting pipe and anesthesia
Bottle；
For filling culture medium in the drosophila culture bottle, the bottle mouth position of the drosophila culture bottle is provided with vapor-permeable plug, described
Connection mouth is additionally provided on the body of drosophila culture bottle, the connection mouth is connected to the inner cavity of the drosophila culture bottle；
One end of the communicating pipe is socketed in the connection mouth, and the other end of the communicating pipe is socketed in the spherical collecting pipe
On, it is provided with valve the communicating pipe between its ends between, the spherical shape collecting pipe is provided with far from the side of the communicating pipe
Permeability cell；
The anesthesia bottle is set to the lower section of the spherical collecting pipe, the bottom of the bottleneck of the anesthesia bottle and the spherical collecting pipe
Connection.
2. drosophila according to claim 1 cultivates collection device, which is characterized in that the bottle mouth position of the anesthesia bottle is provided with
Bottle cap is provided with through-hole on the bottle cap, which is configured with the stopple that can fill in the through-hole, and the centre of the stopple is opened
There is cotton hole, is filled with cotton in the cotton hole.
3. drosophila according to claim 1 cultivates collection device, which is characterized in that it is provided with strainer in the permeability cell,
The aperture of the strainer is no more than 0.5mm.
4. a kind of drosophila according to claim 1 or 3 cultivates collection device, which is characterized in that the permeability cell it is another
End is connected with asepwirator pump.
5. a kind of drosophila according to claim 1 cultivates collection device, which is characterized in that the vapor-permeable plug is rubber
It fills in, air hole is offered on the rubber stopper, the aperture of the air hole is no more than 0.5mm.
6. a kind of drosophila according to claim 1 cultivates collection device, which is characterized in that the outside of the spherical shape collecting pipe
It is provided with moth-killing device.
7. a kind of drosophila according to claim 6 cultivates collection device, which is characterized in that the moth-killing device is that setting exists
The trap lamp contour with the spherical collecting pipe on the outside of the spherical shape collecting pipe.
8. a kind of drosophila according to claim 6 cultivates collection device, which is characterized in that the moth-killing device is to be pasted onto
It is described spherical shape collecting pipe on the outside of can light emitting diode.
9. a kind of drosophila according to claim 1 cultivates collection device, which is characterized in that the spherical shape collecting pipe with it is described
Funnel is provided between anesthesia bottle, the lower end of the funnel is inserted into the bottleneck of the anesthesia bottle, the upper end of the funnel and institute
State the bottom connection of spherical collecting pipe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910080006.7A CN109566544A (en) | 2019-01-28 | 2019-01-28 | A kind of drosophila culture collection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910080006.7A CN109566544A (en) | 2019-01-28 | 2019-01-28 | A kind of drosophila culture collection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109566544A true CN109566544A (en) | 2019-04-05 |
Family
ID=65918162
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910080006.7A Pending CN109566544A (en) | 2019-01-28 | 2019-01-28 | A kind of drosophila culture collection device |
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| Country | Link |
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| CN (1) | CN109566544A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110292026A (en) * | 2019-07-29 | 2019-10-01 | 湖南文理学院 | A kind of drosophila culture collection device |
| CN110301404A (en) * | 2019-07-29 | 2019-10-08 | 湖南文理学院 | A kind of device for collecting virgin drosophila |
| CN111149773A (en) * | 2020-02-17 | 2020-05-15 | 山西大学 | Drosophila resistance strain screening system |
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| US4224898A (en) * | 1979-05-24 | 1980-09-30 | Carolina Biological Supply Company | Method and means for anesthetizing insects |
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| US4106438A (en) * | 1976-09-03 | 1978-08-15 | Nelson Roger K | Method of handling Drosophila |
| US4224898A (en) * | 1979-05-24 | 1980-09-30 | Carolina Biological Supply Company | Method and means for anesthetizing insects |
| CN204518914U (en) * | 2015-03-11 | 2015-08-05 | 中华人民共和国陕西出入境检验检疫局 | The cultivation of fly class and separator in berry fruits |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110292026A (en) * | 2019-07-29 | 2019-10-01 | 湖南文理学院 | A kind of drosophila culture collection device |
| CN110301404A (en) * | 2019-07-29 | 2019-10-08 | 湖南文理学院 | A kind of device for collecting virgin drosophila |
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| CN111149773A (en) * | 2020-02-17 | 2020-05-15 | 山西大学 | Drosophila resistance strain screening system |
| CN111149773B (en) * | 2020-02-17 | 2021-11-19 | 山西大学 | Drosophila resistance strain screening system |
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| PB01 | Publication | ||
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| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20191219 Address after: No. 73 City North East Street, Changzhi, Shanxi Province Applicant after: CHANGZHI University Address before: 046000 Unit 602 households of Building No. 11, 73 North East Street, Changzhi City, Shanxi Province Applicant before: Qin Yongyan |
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190405 |