CN110292026A - A kind of drosophila culture collection device - Google Patents
A kind of drosophila culture collection device Download PDFInfo
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- CN110292026A CN110292026A CN201910688071.8A CN201910688071A CN110292026A CN 110292026 A CN110292026 A CN 110292026A CN 201910688071 A CN201910688071 A CN 201910688071A CN 110292026 A CN110292026 A CN 110292026A
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- 241000255581 Drosophila <fruit fly, genus> Species 0.000 title claims abstract description 66
- 230000007246 mechanism Effects 0.000 claims abstract description 52
- 239000001963 growth medium Substances 0.000 claims abstract description 25
- 238000010438 heat treatment Methods 0.000 claims abstract description 7
- 239000000945 filler Substances 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 2
- 230000035800 maturation Effects 0.000 abstract description 8
- 238000012216 screening Methods 0.000 abstract description 4
- 238000010586 diagram Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 230000003028 elevating effect Effects 0.000 description 3
- 210000002683 foot Anatomy 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 description 2
- 108700005920 Drosophila Step Proteins 0.000 description 1
- 108700009227 Drosophila not Proteins 0.000 description 1
- 241000255588 Tephritidae Species 0.000 description 1
- 206010044565 Tremor Diseases 0.000 description 1
- 230000003542 behavioural effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000000653 nervous system Anatomy 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of drosophilas to cultivate collection device, including shell, and case top is open and is provided with lid, and bottom is provided with the culture mechanism with Temperature and Humidity Control function in the shell；Shell inner top is provided with moving collection mechanism；The culture mechanism includes culture medium, culture medium is fixed at bottom middle position in shell, heating mechanism is provided on bottom left wall in shell, heating mechanism includes electric hot tray, and electric hot tray is fixed in housing sidewall, corresponds in housing sidewall and is provided with block cover on the outside of electric hot tray, when work, drosophila culture is carried out by culture mechanism, culture mechanism has the function of Temperature and Humidity Control, drosophila is facilitated to cultivate；After drosophila culture is mature, by moving collection collect maturation drosophila, moving collection mechanism is located at shell inner top in the present invention, and only drosophila mature enough just can have enough abilities to enter moving collection mechanism, has so just carried out preliminary screening to drosophila；Obtain the drosophila of enough maturations.
Description
Technical field
The invention belongs to drosophila culture technique field, specially a kind of drosophila cultivates collection device.
Background technique
Drosophila is the experiment life of the most-often used exploration life science of scientists, and small drosophila answers human life
Big problem, how life is had an impact including about the time.From very in former people or perhaps scientists
Begin to use drosophila as the important experimental tool of research life science, test that drosophila not only multiplied save rapidly when
Between, and the height of the gene similarity exception of drosophila and people, it is more conducive to our research.Since the 1970s, fruit
Fly is widely used in Developmental Biology research, to understand how a relatively simple fertilized eggs evolve into complicated organism
, including embryonic development, the formation of various adult organs, such as eyes, wing, leg, brain, heart organ be how to develop and
Come.In terms of neurobiological study, drosophila is also a good model, development and advanced mind by its research nervous system
Through activity and behavioral mechanism.Drosophila has been used widely in the every field of biology as model organism.
But currently, the culture collection to drosophila remains defect.When collection, manual operation is improper to be easy to cause fruit
The damage of fly, when culture, temperature affects the culture efficiency of drosophila, brings certain trouble to research.Inventor is comprehensive each
Class factor proposes a kind of drosophila culture collection device.
Summary of the invention
It is an object of the invention to: in order to solve the problems, such as that drosophila cultivates collection, provide a kind of drosophila culture collection device.
The technical solution adopted by the invention is as follows:
A kind of drosophila culture collection device, including shell, case top are open and are provided with lid, bottom in the shell
It is provided with the culture mechanism with Temperature and Humidity Control function；Shell inner top is provided with moving collection mechanism；
When work, drosophila culture is carried out by culture mechanism, culture mechanism has the function of Temperature and Humidity Control, drosophila is facilitated to train
It supports；After drosophila culture is mature, by moving collection collect maturation drosophila, moving collection mechanism is located in shell in the present invention
Top, only drosophila mature enough just can have enough abilities to enter moving collection mechanism, so just carry out just to drosophila
Step screening；Obtain the drosophila of enough maturations.
As the further technical solution of the present invention: the culture mechanism includes culture medium, and culture medium is fixed at shell
Interior bottom middle position is provided with heating mechanism on bottom left wall in shell, and heating mechanism includes electric hot tray, and electric hot tray is fixed
It is corresponded in housing sidewall, in housing sidewall and is provided with block cover on the outside of electric hot tray, prevented drosophila from entering electric hot tray and cause to accidentally injure；Shell
Electric hot tray, which is corresponded to, on external side wall is provided with the electric controller connecting with electric hot tray；It is provided on the housing bottom right side wall
Humidification mechanism, humidification mechanism include atomizer, and dispersive nozzle is provided on atomizer, and dispersive nozzle is arranged in shell；
As further technical solution of the invention: the moving collection mechanism includes surge drum, the setting of shell inner top
There is fixed frame, fixed frame is movably installed in case top, and fixed flange is provided on case top side wall, and fixed frame is connected to solid
Determine on flange；Surge drum is mounted vertically in the middle part of fixed frame, is provided with chimb at the top of surge drum, chimb is overlapped on fixed frame simultaneously
It is connect by screw thread with fixed frame；Cover is provided at the top of surge drum, being provided with after entering for drosophila in the middle part of cover prevents from overflowing
Filler cap out；Trap lamp is symmetrically arranged in surge drum in bottom sidewall, the surge drum bottom end is connected with collection box, collects
Connector is provided at the top of box, connector is sleeved on the outside of surge drum and is threadedly coupled with surge drum；Setting in the collection box
There is the arcotic for anaesthetizing drosophila；
As further technical solution of the invention: being fixedly installed rotary shaft and driving below collection box in the shell
The motor of rotary shaft, rotary shaft lower end are symmetrically arranged with disturbed belt；After drosophila sprouts wings, motor rotation drives waves in disturbed belt,
Promote the drosophila to sprout wings to dance in the air, make it into case top, is convenient for later collection；
As the present invention further technical solution: the collection box bottom is provided with electric telescopic rod, motor bottom with
Electric telescopic rod outer end is fixedly connected；Motor lifting is driven by electric telescopic rod, adjusts disturbed belt and culture medium distance, it is convenient
Disturbance adjusting, the regular elevating movement of electric telescopic rod, that improves disturbed belt waves effect；
As further technical solution of the invention: bottom is provided with shaker mechanism for culture medium in the shell；With
The disturbance of drosophila after emergence forces it to dance in the air to enter upper collection cylinder, is convenient for later collection；The shaker mechanism includes
Backing plate, culture medium are fixed on backing plate, and backing plate left end bottom is connected and fixed column, and fixed column bottom end joins in housing bottom, backing plate
Right end bottom is fixedly supported on housing bottom by multiple adjustable springs, and the housing bottom is provided with open-topped cabinet,
Cam is set in the cabinet, cam is mounted on enclosure top by camshaft and abuts backing plate, is provided with vibration in the cabinet
Dynamic motor, vibrating motor are connect by retarder with camshaft drive；Vibrating motor band moving cam rotates, and cam abuts backing plate band
Dynamic backing plate shake forces it to dance in the air to enter upper collection cylinder, is convenient for later collection so that culture medium is shaken.
As further technical solution of the invention: the housing bottom is provided with blower, is connected with air outlet on blower,
Air outlet protrudes into shell and towards culture medium top, and blower work, which generates air-flow, forces it to dance in the air to enter upper collection
Cylinder is convenient for later collection.
The housing bottom is provided with pedestal, and Its lower side is fixedly installed multiple fixed feets.
In conclusion by adopting the above-described technical solution, the beneficial effects of the present invention are:
1, drosophila culture when the invention works, is carried out by culture mechanism, culture mechanism has the function of Temperature and Humidity Control, side
Just drosophila is cultivated；After drosophila culture is mature, by moving collection collect maturation drosophila, moving collection mechanism position in the present invention
In shell inner top, only only enough mature drosophilas just can have enough abilities to enter moving collection mechanism, so just to drosophila
Preliminary screening is carried out；Obtain the drosophila of enough maturations.
2, the present invention is further fixedly installed rotary shaft below collection box and drives the motor of rotary shaft, rotary shaft
Lower end is symmetrically arranged with disturbed belt；After drosophila sprouts wings, motor rotation drives waves in disturbed belt, and the drosophila to sprout wings is promoted to dance in the air,
Case top is made it into, later collection is convenient for.
3, the present invention is further provided with electric telescopic rod, motor bottom and electric telescopic rod outer end in collection box bottom
It is fixedly connected；Motor lifting is driven by electric telescopic rod, disturbed belt and culture medium distance is adjusted, facilitates disturbance adjusting, it is electronic
The regular elevating movement of telescopic rod, that improves disturbed belt waves effect.
4, the present invention is further provided with shaker mechanism for culture medium, and vibrating motor is provided in the cabinet, shakes
Dynamic motor is connect by retarder with camshaft drive；Vibrating motor band moving cam rotates, and cam abuts backing plate and backing plate is driven to tremble
It is dynamic, so that culture medium is shaken, forces it to dance in the air to enter upper collection cylinder, be convenient for later collection.
5, the present invention is further provided with blower in housing bottom, air outlet is connected on blower, air outlet protrudes into shell
In vivo and towards culture medium top, blower work, which generates air-flow, forces it to dance in the air to enter upper collection cylinder, is convenient for subsequent receipts
Collection.
Detailed description of the invention
Fig. 1 is one structure schematic diagram of the embodiment of the present invention；
Fig. 2 is the internal structure simplified schematic diagram of the embodiment of the present invention one；
Fig. 3 is two structure schematic diagram of the embodiment of the present invention；
Fig. 4 is three structure schematic diagram of the embodiment of the present invention；
Fig. 5 is four structure schematic diagram of the embodiment of the present invention；
Fig. 6 is five structure schematic diagram of the embodiment of the present invention.
Marked in the figure: 1, shell；2, lid；3, electric controller；4, pedestal；5, fixed feet；6, atomizer；7, fixation is convex
Edge；8, fixed frame；9, surge drum；10, cover；11, filler cap；12, chimb；13, trap lamp；14, connector；15, collection box；
16, dispersive nozzle；17, culture medium；18, block cover；19, electric hot tray；20, motor；21, rotary shaft；22, disturbed belt；23, electronic to stretch
Contracting bar；24, backing plate；25, fixed column；26, support spring；27, cabinet；28, vibrating motor；29, retarder；30, camshaft；31
Cam；32, blower；33, air outlet.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to the accompanying drawings and embodiments, right
The present invention is further elaborated.It should be appreciated that described herein, specific examples are only used to explain the present invention, not
For limiting the present invention.
Embodiment one, referring to Fig.1-2, a kind of drosophila culture collection device, including shell 1,1 top opening of shell and setting
There is lid 2, bottom is provided with the culture mechanism with Temperature and Humidity Control function in the shell 1；1 inner top of shell is provided with work
Dynamic collecting mechanism；The culture mechanism includes culture medium 17, and culture medium 17 is fixed at bottom middle position, shell in shell 1
Heating mechanism is provided in body 1 on bottom left wall, heating mechanism includes electric hot tray 19, and electric hot tray 19 is fixed on 1 side wall of shell
On, it is corresponded on 1 side wall of shell and is provided with block cover 18 on the outside of electric hot tray 19, prevented drosophila from entering electric hot tray 19 and cause to accidentally injure；Shell 1
Electric hot tray 19 is corresponded on lateral wall is provided with the electric controller 3 connecting with electric hot tray 19；It is set on the 1 bottom, right side wall of shell
It is equipped with humidification mechanism, humidification mechanism includes atomizer 6, and dispersive nozzle 16 is provided on atomizer 6, and dispersive nozzle 16 is arranged in shell
In body 1；The moving collection mechanism includes surge drum 9, and 1 inner top of shell is provided with fixed frame 8, and fixed frame 8 is movably installed in
At the top of shell 1, it is provided with fixed flange 7 in 1 top sidewall of shell, fixed frame 8 is connected in fixed flange 7；Surge drum 9 is vertical
It is mounted on 8 middle part of fixed frame, chimb 12 is provided at the top of surge drum 9, chimb 12 is overlapped on fixed frame 8 and by screw thread and admittedly
Determine the connection of frame 8；It is provided with cover 10 at the top of surge drum 9, the leakage for preventing from overflowing is provided with after entering for drosophila in the middle part of cover 10
Bucket cover 11；Trap lamp 13 is symmetrically arranged in surge drum 9 in bottom sidewall, 9 bottom end of surge drum is connected with collection box 15, receives
Connector 14 is provided at the top of collection box 15, connector 14 is sleeved on 9 outside of surge drum and is threadedly coupled with surge drum 9；The receipts
The arcotic for anaesthetizing drosophila is provided in collection box 15.
Embodiment two: referring to Fig. 3, the present embodiment increases following technical characteristic on the basis of example 1:
Rotary shaft 21 is fixedly installed in the shell 1 below collection box 15 and drives the motor 20 of rotary shaft 21, rotation
21 lower end of axis is symmetrically arranged with disturbed belt 22；After drosophila sprouts wings, the rotation of motor 20 drives waves in disturbed belt 22, promotes emergence
Drosophila dances in the air, and makes it into 1 top of shell, is convenient for later collection.
Embodiment three: referring to Fig. 4, the present embodiment increases following technical characteristic on the basis of example 2:
15 bottom of collection box is provided with electric telescopic rod 23, the fixed company in 20 bottom of motor and 23 outer end of electric telescopic rod
It connects；It drives motor 20 to go up and down by electric telescopic rod 23, adjusts disturbed belt 22 and 17 distance of culture medium, facilitate disturbance adjusting, electricity
The regular elevating movement of dynamic telescopic rod 23, that improves disturbed belt 22 waves effect.
Example IV: referring to Fig. 5, the present embodiment increases following technical characteristic on the basis of example 1:
Bottom is provided with shaker mechanism for culture medium 17 in the shell 1；The shaker mechanism includes backing plate 24, culture
Base 17 is fixed on backing plate 24, and 24 left end bottom of backing plate is connected and fixed column 25, and the handover of 25 bottom end of fixed column is in 1 bottom of shell, pad
24 right end bottom of plate is fixedly supported on 1 bottom of shell by multiple adjustable springs, and 1 bottom of shell is provided with open-topped
Cabinet 27, cam 31 is set in the cabinet 27, and cam 31 is mounted at the top of cabinet 27 by 31 axis 30 of cam and abuts backing plate
24, vibrating motor 28 is provided in the cabinet 27, vibrating motor 28 is sequentially connected by retarder 29 and 31 axis 30 of cam；Vibration
Dynamic motor 28 is rotated with moving cam 31, and cam 31 abuts backing plate 24 and backing plate 24 is driven to shake, so that culture medium 17 is shaken, forces it
It dances in the air to enter upper collection cylinder 9, is convenient for later collection.
Embodiment five: referring to Fig. 6, the present embodiment increases following technical characteristic on the basis of example 1:
1 bottom of shell is provided with blower 32, and air outlet 33 is connected on blower 32, and air outlet 33 protrudes into shell 1
And towards 17 top of culture medium, the work of blower 32, which generates air-flow, forces it to dance in the air to enter upper collection cylinder 9, is convenient for subsequent receipts
Collection.
Working principle of the present invention: when work, drosophila culture is carried out by culture mechanism, culture mechanism has Temperature and Humidity Control
Function facilitates drosophila to cultivate；After drosophila culture is mature, by moving collection collect maturation drosophila, activity is received in the present invention
Collecting mechanism is located at shell inner top, and only drosophila mature enough just can have enough abilities to enter moving collection mechanism, so
Preliminary screening just has been carried out to drosophila；Obtain the drosophila of enough maturations.
In the description of the present invention, it should be noted that term " center ", "upper", "lower", "left", "right", "vertical",
The orientation or positional relationship of the instructions such as "horizontal", "inner", "outside" be based on the orientation or positional relationship shown in the drawings, merely to
Convenient for description the present invention and simplify description, rather than the device or element of indication or suggestion meaning must have a particular orientation,
It is constructed and operated in a specific orientation, therefore is not considered as limiting the invention；Term " first ", " second ", " third "
It is used for descriptive purposes only and cannot be understood as indicating or suggesting relative importance；In addition, unless otherwise specific regulation and limit
Fixed, term " installation ", " connected ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, be also possible to detachably connect
It connects, or is integrally connected；It can be mechanical connection, be also possible to be electrically connected；It can be directly connected, intermediate matchmaker can also be passed through
Jie is indirectly connected, and can be the connection inside two elements.It for the ordinary skill in the art, can be with concrete condition
Understand the concrete meaning of above-mentioned term in the present invention.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (8)
1. a kind of drosophila cultivates collection device, including shell (1), shell (1) top opening and it is provided with lid (2), the shell
Body (1) interior bottom is provided with culture mechanism；Shell (1) inner top is provided with moving collection mechanism.
2. a kind of drosophila as described in claim 1 cultivates collection device, it is characterised in that: the culture mechanism includes culture medium
(17), culture medium (17) is fixed at the interior bottom middle position of shell (1), is provided with and adds on the interior bottom left wall of shell (1)
Heat engine structure, heating mechanism include electric hot tray (19), and electric hot tray (19) is fixed on shell (1) side wall, corresponding on shell (1) side wall
Block cover (18) are provided on the outside of electric hot tray (19)；Electric hot tray (19) is corresponded on shell (1) lateral wall to be provided with and electric hot tray (19)
The electric controller (3) of connection；It is provided with humidification mechanism on shell (1) the bottom, right side wall, humidification mechanism includes atomizer
(6), it is provided with dispersive nozzle (16) on atomizer (6), dispersive nozzle (16) setting is in shell (1).
3. a kind of drosophila as described in claim 1 cultivates collection device, it is characterised in that: the moving collection mechanism includes receiving
Collect cylinder (9), shell (1) inner top is provided with fixed frame (8), and fixed frame (8) is movably installed at the top of shell (1), shell (1) top
It is provided on portion's side wall fixed flange (7), fixed frame (8) is connected on fixed flange (7)；Surge drum (9) is mounted vertically in solid
Determine in the middle part of frame (8), be provided with chimb (12) at the top of surge drum (9), chimb (12) be overlapped on fixed frame (8) and by screw thread with
Fixed frame (8) connection；It is provided with cover (10) at the top of surge drum (9), is provided with filler cap (11) in the middle part of cover (10)；Surge drum
(9) it is symmetrically arranged in interior bottom sidewall trap lamp (13), surge drum (9) bottom end is connected with collection box (15).
4. a kind of drosophila as claimed in claim 3 cultivates collection device, it is characterised in that: setting at the top of the collection box (15)
Have connector (14), connector (14) is sleeved on the outside of surge drum (9) and is threadedly coupled with surge drum (9)；The collection box
(15) arcotic is provided in.
5. a kind of drosophila as claimed in claim 3 cultivates collection device, it is characterised in that: the interior collection box of the shell (1)
(15) lower section is fixedly installed rotary shaft (21) and drives the motor (20) of rotary shaft (21), and rotary shaft (21) lower end is symmetrical arranged
There are disturbed belt (22).
6. a kind of drosophila as claimed in claim 5 cultivates collection device, it is characterised in that: collection box (15) the bottom setting
Have electric telescopic rod (23), motor (20) bottom is fixedly connected with electric telescopic rod (23) outer end.
7. a kind of drosophila as described in claim 1~6 is any cultivates collection device, it is characterised in that: the interior bottom of the shell (1)
Portion is provided with shaker mechanism for culture medium (17)；The shaker mechanism includes backing plate (24), and culture medium (17) is fixed on backing plate
(24) on, backing plate (24) left end bottom is connected and fixed column (25), and fixed column (25) bottom end joins in shell (1) bottom, backing plate
(24) right end bottom is fixedly supported on shell (1) bottom by multiple adjustable springs, and shell (1) bottom is provided with top and opens
The cabinet (27) of mouth, the cabinet (27) is interior to be set with cam (31), and cam (31) is mounted on machine by cam (31) axis (30)
Backing plate (24) are abutted at the top of case (27), are provided with vibrating motor (28) in the cabinet (27), vibrating motor (28) passes through deceleration
Device (29) and cam (31) axis (30) are sequentially connected.
8. a kind of drosophila as claimed in claim 7 cultivates collection device, it is characterised in that: shell (1) bottom is provided with
Blower (32) is connected with air outlet (33) on blower (32), and air outlet (33) is protruded into shell (1) and pushed up towards culture medium (17)
Portion.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910688071.8A CN110292026B (en) | 2019-07-29 | 2019-07-29 | Fruit fly culture and collection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910688071.8A CN110292026B (en) | 2019-07-29 | 2019-07-29 | Fruit fly culture and collection device |
Publications (2)
| Publication Number | Publication Date |
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| CN110292026A true CN110292026A (en) | 2019-10-01 |
| CN110292026B CN110292026B (en) | 2021-07-30 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910688071.8A Active CN110292026B (en) | 2019-07-29 | 2019-07-29 | Fruit fly culture and collection device |
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| CN (1) | CN110292026B (en) |
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| CN111436400A (en) * | 2020-03-17 | 2020-07-24 | 南阳师范学院 | Laboratory fruit fly culture bottle interface device |
| CN111436400B (en) * | 2020-03-17 | 2021-09-24 | 南阳师范学院 | Laboratory fruit fly culture bottle interface device |
| CN112106732A (en) * | 2020-08-19 | 2020-12-22 | 浙江蓝美技术股份有限公司 | Rapid propagation method of drosophila melanogaster pupae |
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