CN112106732A - Rapid propagation method of drosophila melanogaster pupae - Google Patents
Rapid propagation method of drosophila melanogaster pupae Download PDFInfo
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- CN112106732A CN112106732A CN202010837866.3A CN202010837866A CN112106732A CN 112106732 A CN112106732 A CN 112106732A CN 202010837866 A CN202010837866 A CN 202010837866A CN 112106732 A CN112106732 A CN 112106732A
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- 241000255601 Drosophila melanogaster Species 0.000 title claims abstract description 39
- 238000000034 method Methods 0.000 title claims abstract description 19
- 241000255588 Tephritidae Species 0.000 claims abstract description 34
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 241000255581 Drosophila <fruit fly, genus> Species 0.000 claims abstract description 13
- 238000002360 preparation method Methods 0.000 claims abstract description 5
- 239000007788 liquid Substances 0.000 claims description 20
- 238000007599 discharging Methods 0.000 claims description 13
- 239000000843 powder Substances 0.000 claims description 10
- 239000008213 purified water Substances 0.000 claims description 10
- 238000003756 stirring Methods 0.000 claims description 10
- 238000005303 weighing Methods 0.000 claims description 10
- 239000000463 material Substances 0.000 claims description 6
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical compound [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 claims description 5
- 229920001817 Agar Polymers 0.000 claims description 5
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 5
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 5
- 229930006000 Sucrose Natural products 0.000 claims description 5
- 240000008042 Zea mays Species 0.000 claims description 5
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 5
- 239000008272 agar Substances 0.000 claims description 5
- 238000010411 cooking Methods 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 5
- 235000005822 corn Nutrition 0.000 claims description 5
- 235000013312 flour Nutrition 0.000 claims description 5
- 238000010438 heat treatment Methods 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 229940069338 potassium sorbate Drugs 0.000 claims description 5
- 235000010241 potassium sorbate Nutrition 0.000 claims description 5
- 239000004302 potassium sorbate Substances 0.000 claims description 5
- 229960004793 sucrose Drugs 0.000 claims description 5
- 238000004140 cleaning Methods 0.000 claims description 4
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 3
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 3
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 3
- 239000011425 bamboo Substances 0.000 claims description 3
- 230000003203 everyday effect Effects 0.000 claims description 3
- 238000009423 ventilation Methods 0.000 claims description 3
- 206010002091 Anaesthesia Diseases 0.000 claims description 2
- 230000037005 anaesthesia Effects 0.000 claims description 2
- 230000001902 propagating effect Effects 0.000 claims description 2
- 241001330002 Bambuseae Species 0.000 claims 2
- 230000000249 desinfective effect Effects 0.000 claims 1
- 230000019617 pupation Effects 0.000 abstract 1
- 238000005520 cutting process Methods 0.000 description 10
- 238000009395 breeding Methods 0.000 description 9
- 230000001488 breeding effect Effects 0.000 description 8
- 238000005452 bending Methods 0.000 description 4
- 241000382353 Pupa Species 0.000 description 3
- 241000238631 Hexapoda Species 0.000 description 2
- 241001481304 Vespoidea Species 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 230000032669 eclosion Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 241000255925 Diptera Species 0.000 description 1
- 241000282414 Homo sapiens Species 0.000 description 1
- 241000500891 Insecta Species 0.000 description 1
- 244000082204 Phyllostachys viridis Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/0333—Genetically modified invertebrates, e.g. transgenic, polyploid
- A01K67/0337—Genetically modified Arthropods
- A01K67/0339—Genetically modified insects, e.g. Drosophila melanogaster, medfly
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Catching Or Destruction (AREA)
- Feeding And Watering For Cattle Raising And Animal Husbandry (AREA)
Abstract
The invention discloses a rapid propagation method of drosophila melanogaster pupae, which comprises a) propagation preparation, b) pupation of drosophila melanogaster, and c) collection of drosophila melanogaster pupae, wherein the propagation tube comprises a tube body, a tube bottom and a tube cover, the outer walls of the upper end and the lower end of the tube body are respectively provided with a second external thread and a first external thread, the inner wall of the lower end of the tube cover and the inner wall of the upper end of the tube bottom are respectively provided with a second internal thread and a first internal thread which are matched with the second external thread and the first external thread, the side wall of the tube body and the position close to the top end are provided with a feed channel which is plugged by a feed plug, the top end of the tube cover is provided with a plurality of openings, a gauze is arranged in each opening, after the drosophila larva of drosophila melanogaster pupae is pupae, the tube body can be independently replaced, then the drosophila melanogaster pupae is flushed onto a screen by water flow and a brush without taking, time and labor are saved, the efficiency is high, the operation is convenient, and the problem of rapid and mass supply of the fruit fly pupae is solved.
Description
[ technical field ] A method for producing a semiconductor device
The invention relates to the technical field of fruit fly breeding, in particular to the technical field of a rapid breeding method of drosophila melanogaster pupae.
[ background of the invention ]
Drosophila refers to insects of the Drosophilaceae family, Diptera, class Insecta, with over 4000 species being described. In recent years, the loss of fruit flies as pests to the fruit industry has become more serious, and so effective control of fruit flies to reduce the economic loss caused thereby has become more urgent. Drosophila melanogaster, a common model organism, has been used extensively in genetics and developmental biology. At present, the value of drosophila melanogaster to human beings is researched no matter the drosophila melanogaster is taken as a model insect; the drosophila melanogaster is also taken as a pest target, and an effective control method of the drosophila melanogaster is researched; the drosophila melanogaster is further utilized to produce the drosophila melanogaster parasitic wasps, and a large amount of drosophila melanogaster pupae are needed when the drosophila melanogaster parasitic wasps are applied to production. However, an effective method and device for producing and rapidly collecting the drosophila pupae are not available at present, and need to be solved urgently.
[ summary of the invention ]
The invention aims to solve the problems in the prior art, provides a rapid propagation method of drosophila melanogaster pupae, is time-saving, labor-saving, efficient and convenient, and solves the problem of rapid and mass supply of drosophila melanogaster pupae.
In order to achieve the purpose, the invention is realized by the following technical scheme:
a rapid propagation method of drosophila melanogaster pupae comprises the following steps:
a) preparing propagation: respectively cleaning and sterilizing the cylinder body, the cylinder bottom and the cylinder cover of the breeding cylinder, laying fruit fly feed in the cylinder bottom, and respectively connecting the upper end and the lower end of the cylinder body with the cylinder bottom and the cylinder cover;
b) pupating fruit flies: feeding the primarily emerged drosophila melanogaster into an inner cavity of a propagation cylinder along a feeding channel positioned on one side of a cylinder body after anesthesia, and propagating for 10-12 days at the temperature of 23-27 ℃;
c) collecting fruit fly pupae: and (3) from the 5 th day of reproduction, replacing the new barrel body for 1-5 times every day, placing the taken-down old barrel body on a screen, flushing the drosophila pupae positioned on the inner wall of the barrel body by using water flow and a brush, and draining the drosophila pupae at a ventilation position to obtain a pupa finished product.
Preferably, in the step a), the preparation method of the drosophila feed comprises the steps of firstly weighing 40-60 parts by weight of corn flour, 20-40 parts by weight of sucrose, 15-25 parts by weight of yeast powder and 80-120 parts by weight of purified water, uniformly stirring to form a first feed liquid, then weighing 8-12 parts by weight of agar powder and 900-1100 parts by weight of purified water, uniformly heating and stirring to form a second feed liquid, mixing and cooking the first feed liquid and the second feed liquid, then adding 0.4-0.6 part by weight of potassium sorbate, and cooling to room temperature.
Preferably, in the step a), the fruit fly feed is filled to a position 0.5-1 cm away from the top opening of the barrel bottom.
Preferably, in step b), CO is used2The drosophila melanogaster was anesthetized.
Preferably, in the step c), the mesh number of the screen is 80-120 meshes.
Preferably, the breeding barrel comprises a barrel body, a barrel bottom and a barrel cover, second external threads and first external threads are respectively arranged at the outer walls of the upper end and the lower end of the barrel body, second internal threads and first internal threads matched with the second external threads and the first external threads are respectively arranged at the inner walls of the lower end of the barrel cover and the upper end of the barrel bottom, a feeding channel blocked by a feeding rubber plug is arranged at the position, close to the top end, of the side wall of the barrel body, a plurality of openings are formed in the top end of the barrel cover, and a gauze is arranged in the openings.
Preferably, the inner wall of the cylinder bottom and the position close to the top are provided with annular flanges, the top surfaces of the annular flanges are provided with first annular grooves, first rubber rings are arranged in the first annular grooves, the edge of the bottom surface of the cover plate of the cylinder cover is provided with second annular grooves, and second rubber rings are arranged in the second annular grooves.
Preferably, a spun yarn mesh bag is arranged in the barrel bottom, a plurality of convex blocks are arranged on the inner wall of the barrel bottom and close to the top of the barrel bottom, cutting grooves are formed in the convex blocks, the bending direction of the cutting grooves is changed continuously, a plurality of corners are formed, a plurality of hanging ropes are arranged at the top end of the spun yarn mesh bag, and the hanging ropes are clamped into the cutting grooves respectively and are clamped and fixed in the convex blocks.
Preferably, a discharge channel blocked by a discharge rubber plug is arranged on the side wall of the barrel body and close to the bottom end of the barrel body, the discharge channel further comprises a net bag and a guide sleeve, one end of the guide sleeve is sleeved outside the discharge channel, a groove is formed in the other end of the guide sleeve, an elastic ring is arranged in the groove, and an opening of the net bag is bound on the guide sleeve by the elastic ring.
Preferably, the center of the cover plate of the cylinder cover is provided with a material ejecting channel plugged by the perforated rubber plug, a fly driving swatter is inserted in the perforated rubber plug and comprises a supporting rod, a handle and a net swatter, the handle and the net swatter are respectively fixed at the upper end and the lower end of the supporting rod, the bottom end of the supporting rod is provided with an expansion part matched with the aperture of the central through hole of the perforated rubber plug, and the lower end of the expansion part is provided with a limiting block.
The invention has the beneficial effects that: according to the fruit fly pupa breeding device, the breeding barrel with the barrel body, the barrel bottom and the barrel cover which can be separated from each other is arranged, so that the barrel body can be independently replaced after fruit fly larvae pupate, then the fruit fly pupae are flushed onto the screen by using water flow and the hairbrush, the fruit fly pupae do not need to be manually taken down one by using tweezers, time and labor are saved, the operation is efficient and convenient, and the problem of rapid and large-scale supply of the fruit fly pupae is solved; the fine yarn mesh bag is arranged in the barrel bottom, so that the contact between the fruit fly feed and the barrel bottom can be reduced, and the cleaning is convenient; the plurality of bumps are arranged on the inner wall of the barrel bottom and close to the top, and the cutting grooves which can change the bending direction continuously are arranged in the bumps, so that the hanging rope can be clamped quickly; fix the string bag on the material discharging channel through setting up the guide pin bushing, utilize and drive the fly bat and move down gradually to catch up into the string bag in the fruit fly that will have feathered from the stack shell, in order to carry out next reproduction.
The features and advantages of the present invention will be described in detail by embodiments in conjunction with the accompanying drawings.
[ description of the drawings ]
FIG. 1 is a front cross-sectional view of a propagation cylinder of the present invention;
FIG. 2 is a front cross-sectional view of the propagation cylinder of the present invention with the discharge rubber plug removed and the string bag and guide sleeve installed;
FIG. 3 is a front cross-sectional view of the body of the propagation cylinder of the present invention;
FIG. 4 is a front cross-sectional view of the bottom of the propagation cylinder of the present invention;
FIG. 5 is a top view of the bottom of the propagation cylinder of the present invention;
FIG. 6 is a front cross-sectional view of the lid of the propagation cylinder of the present invention;
FIG. 7 is a top view of the lid of the propagation cylinder of the present invention;
FIG. 8 is a front cross-sectional view of a fly swatter, in accordance with the invention, of a reproductive cartridge.
In the figure: 1-cylinder body, 11-first external thread, 12-second external thread, 13-feeding channel, 14-feeding rubber plug, 15-discharging channel, 16-discharging rubber plug, 2-cylinder bottom, 21-first internal thread, 22-annular flange, 23-first rubber ring, 24-lug, 241-cutting groove, 3-cylinder cover, 31-second internal thread, 32-second rubber ring, 33-opening, 34-gauze, 35-material pushing channel, 36-rubber plug with hole, 4-fly driving swatter, 41-supporting rod, 411-expansion part, 412-limiting block, 42-handle, 43-net swatter, 5-string bag and 6-guide sleeve.
[ detailed description ] embodiments
The first embodiment is as follows:
breed a section of thick bamboo and include stack shell 1, bobbin base 2 and cover 3, the upper and lower both ends outer wall punishment of stack shell 1 do not is equipped with second external screw thread 12 and first external screw thread 11, the lower extreme inner wall of cover 3 and the upper end inner wall punishment of stack base 2 do not are equipped with and second external screw thread 12 and first external screw thread 11 matched with second internal thread 31 and first internal thread 21, the lateral wall of stack shell 1 just is close to top department and is equipped with the feed channel 13 by 14 shutoff of feeding plug, the top of cover 3 is equipped with a plurality of opening 33, be equipped with gauze 34 in the opening 33.
The inner wall of barrel head 2 just is close to top department and is equipped with annular flange 22, the top surface of annular flange 23 is equipped with first ring channel, first ring channel embeds there is first rubber ring 23, the apron bottom surface edge of cover 3 is equipped with the second ring channel, the second ring channel embeds there is second rubber ring 32.
The bobbin base 2 is internally provided with a spun yarn mesh bag, the inner wall of the bobbin base 2 is provided with a plurality of convex blocks 24 close to the top, the convex blocks 24 are internally provided with cutting grooves 241, the bending direction of the cutting grooves 241 is constantly changed, a plurality of corners are formed, the top end of the spun yarn mesh bag is provided with a plurality of hanging ropes, and the hanging ropes are respectively clamped into the cutting grooves 241 and clamped and fixed in the convex blocks 24.
The side wall of the barrel body 1 and the position close to the bottom end are provided with a discharging channel 15 blocked by a discharging rubber plug 16, the barrel further comprises a net bag 5 and a guide sleeve 6, one end of the guide sleeve 6 is sleeved outside the discharging channel 15, the other end of the guide sleeve 6 is provided with a groove, an elastic ring is arranged in the groove, and the elastic ring binds an opening of the net bag 5 on the guide sleeve 6.
The utility model discloses a fly swatter, including cylinder cover 3, handle 42, net bat 43, bracing piece 41, expansion portion 411 that the bottom of bracing piece 41 is equipped with and suits with the central through-hole aperture of foraminiferous plug 36, the lower extreme of expansion portion 411 is equipped with stopper 412, the liftout way 35 of 36 shutoff of foraminiferous plug is equipped with by foraminiferous plug 36, perforated plug 36 interpolation has driven fly swatter 4, it includes bracing piece 41, handle 42 and net bat 43 to drive fly swatter 4, handle 42 and net bat 43 are fixed respectively at the upper and lower both ends of bracing piece 41. Wherein the net flap 43 is arranged obliquely so that the end located far from the discharge channel 15 is lower than the end located close to the discharge channel 15.
The rapid propagation of the drosophila melanogaster pupae by adopting the propagation cylinder comprises the following steps:
a) preparing propagation:
firstly, weighing 50 parts of corn flour, 30 parts of cane sugar, 20 parts of yeast powder and 100 parts of purified water according to the weight proportion, uniformly stirring to form a first feed liquid, then weighing 10 parts of agar powder and 900 parts of purified water according to the weight proportion, heating and uniformly stirring to form a second feed liquid, mixing and cooking the first feed liquid and the second feed liquid, then adding 0.5 part of potassium sorbate according to the weight proportion, and cooling to room temperature to prepare the drosophila feed. The breeding cylinder is cleaned and sterilized, then the spun yarn mesh bag is placed in the inner cavity of the cylinder bottom 2, and a plurality of hanging ropes at the top are respectively clamped into the cutting grooves 241 and clamped and fixed in the convex blocks 24. Then, fruit fly feed is laid in the spun yarn mesh bag, the fruit fly feed is filled to a position 0.5cm away from the top opening of the cylinder bottom 2, and the upper end and the lower end of the cylinder body 1 are respectively connected with the cylinder bottom 2 and the cylinder cover 3.
b) Pupating fruit flies:
subjecting the primarily emerged Drosophila melanogaster to CO2After anaesthetizing, the mixture is put into a 1.6cm finger-shaped tube, then the feeding rubber plug 14 is taken down, and the drosophila melanogaster is sent into a propagation cylinder along the feeding channel 13In the lumen of (2), at a temperature of 25 ℃ for 10 days.
c) Collecting fruit fly pupae:
because the drosophila melanogaster does not like to pupate on the surface of the wet drosophila feed, the offspring drosophila melanogaster larvae can climb up to the inner wall of the barrel body 1 to pupate on about 5 days of reproduction. From the 5 th day of reproduction, the new barrel body is replaced 11-5 times every day, the taken-down old barrel body 1 is placed on a 100-mesh screen, the fruit fly pupae on the inner wall of the barrel body 1 are washed down by water flow and a brush, and the fruit fly pupae are drained in a ventilation place to obtain a pupae finished product. In the process of taking pupae, firstly, the discharging rubber plug 16 is taken down from the discharging channel 15, then the guide sleeve 6 is sleeved on the discharging channel 15, and then the opening of the net bag 5 is bound at the other end of the guide sleeve 6 through the elastic ring. The perforated plug 36 is then pulled from the ejector channel 35 and the perforated plug 36 is moved upward, away from the expansion 411 of the support rod 41. Then, the handle 42 and the rubber plug 36 with the hole are held, the net racket 43 is driven by the support rod 41 to move downwards slowly, and the eclosion black drosophila is driven to enter the net bag 5 through the material outlet channel 15. The net racket 43 can be moved up and down for several times until almost all the eclosion black drosophila leaves the cylinder body 1, and then the cylinder body 1 is separated from the cylinder bottom 2 and the cylinder cover 3 respectively.
Example two:
the preparation method of the drosophila feed comprises the steps of weighing 40 parts of corn flour, 20 parts of cane sugar, 15 parts of yeast powder and 100 parts of purified water according to the weight proportion, uniformly stirring to form a first feed liquid, then weighing 8 parts of agar powder and 900 parts of purified water according to the weight proportion, heating and uniformly stirring to form a second feed liquid, mixing and cooking the first feed liquid and the second feed liquid, then adding 0.4 part of potassium sorbate according to the weight proportion, and cooling to room temperature. The other steps are the same as those of the first embodiment.
Example three:
the preparation method of the drosophila feed comprises the steps of weighing 60 parts of corn flour, 40 parts of cane sugar, 25 parts of yeast powder and 120 parts of purified water according to the weight proportion, uniformly stirring to form a first feed liquid, then weighing 12 parts of agar powder and 1100 parts of purified water according to the weight proportion, heating and uniformly stirring to form a second feed liquid, mixing and cooking the first feed liquid and the second feed liquid, then adding 0.6 part of potassium sorbate according to the weight proportion, and cooling to room temperature. The other steps are the same as those of the first embodiment.
According to the fruit fly pupa breeding device, the breeding barrel with the barrel body, the barrel bottom and the barrel cover which can be separated from each other is arranged, so that the barrel body can be independently replaced after fruit fly larvae pupate, then the fruit fly pupae are flushed onto the screen by using water flow and the hairbrush, the fruit fly pupae do not need to be manually taken down one by using tweezers, time and labor are saved, the operation is efficient and convenient, and the problem of rapid and large-scale supply of the fruit fly pupae is solved; the fine yarn mesh bag is arranged in the barrel bottom, so that the contact between the fruit fly feed and the barrel bottom can be reduced, and the cleaning is convenient; the plurality of bumps are arranged on the inner wall of the barrel bottom and close to the top, and the cutting grooves which can change the bending direction continuously are arranged in the bumps, so that the hanging rope can be clamped quickly; fix the string bag on the material discharging channel through setting up the guide pin bushing, utilize and drive the fly bat and move down gradually to catch up into the string bag in the fruit fly that will have feathered from the stack shell, in order to carry out next reproduction.
The above embodiments are illustrative of the present invention, and are not intended to limit the present invention, and any simple modifications of the present invention are within the scope of the present invention.
Claims (10)
1. A rapid propagation method of drosophila melanogaster pupae is characterized in that: the method comprises the following steps:
a) preparing propagation: respectively cleaning and disinfecting a cylinder body (1), a cylinder bottom (2) and a cylinder cover (3) of a propagation cylinder, laying fruit fly feed in the cylinder bottom (2), and respectively connecting the upper end and the lower end of the cylinder body (1) with the cylinder bottom (2) and the cylinder cover (3);
b) pupating fruit flies: feeding the primarily emerged drosophila melanogaster into an inner cavity of a propagation cylinder along a feeding channel (13) positioned on one side of a cylinder body (1) after anesthesia, and propagating for 10-12 days at the temperature of 23-27 ℃;
c) collecting fruit fly pupae: from the 5 th day of reproduction, the new barrel body (1) is replaced 1-5 times every day, the taken-down old barrel body (1) is placed on a screen, the fruit fly pupae on the inner wall of the barrel body (1) are washed down by water flow and a brush, and the fruit fly pupae are drained in a ventilation place to obtain a pupae finished product.
2. The method for rapid propagation of fly pupae of drosophila melanogaster according to claim 1, wherein: in the step a), the preparation method of the drosophila feed comprises the steps of firstly weighing 40-60 parts by weight of corn flour, 20-40 parts by weight of cane sugar, 15-25 parts by weight of yeast powder and 80-120 parts by weight of purified water, uniformly stirring to form a first feed liquid, then weighing 8-12 parts by weight of agar powder and 900-1100 parts by weight of purified water, uniformly heating and stirring to form a second feed liquid, mixing and cooking the first feed liquid and the second feed liquid, then adding 0.4-0.6 part by weight of potassium sorbate, and cooling to room temperature.
3. The method for rapid propagation of fly pupae of drosophila melanogaster according to claim 1, wherein: in the step a), the fruit fly feed is filled to a position 0.5-1 cm away from the top opening of the cylinder bottom (2).
4. The method for rapid propagation of fly pupae of drosophila melanogaster according to claim 1, wherein: in the step b), CO is adopted2The drosophila melanogaster was anesthetized.
5. The method for rapid propagation of fly pupae of drosophila melanogaster according to claim 1, wherein: in the step c), the mesh number of the screen is 80-120 meshes.
6. The method for rapid propagation of fly pupae of drosophila melanogaster according to claim 1, wherein: breed the section of thick bamboo including stack shell (1), bobbin base (2) and cover (3), the upper and lower both ends outer wall punishment of stack shell (1) do not is equipped with second external screw thread (12) and first external screw thread (11), the lower extreme inner wall of cover (3) and the upper end inner wall punishment of stack base (2) do not are equipped with second internal thread (31) and first internal thread (21) with second external screw thread (12) and first external screw thread (11) matched with, the lateral wall of stack shell (1) just is close to top department and is equipped with feed channel (13) by feeding plug (14) shutoff, the top of cover (3) is equipped with a plurality of opening (33), be equipped with gauze (34) in opening (33).
7. The method for rapid propagation of fly pupae of drosophila melanogaster according to claim 6, wherein: the inner wall of barrel head (2) just is close to top department and is equipped with annular flange (22), the top surface of annular flange (23) is equipped with first ring channel, first ring channel embeds there is first rubber ring (23), the apron bottom surface edge of cover (3) is equipped with the second ring channel, the second ring channel embeds there is second rubber ring (32).
8. The method for rapid propagation of fly pupae of drosophila melanogaster according to claim 6, wherein: the built-in spun yarn pocket that has in bobbin base (2), the inner wall of bobbin base (2) just is close to top department and is equipped with a plurality of lug (24), be equipped with grooving (241) in lug (24), grooving (241) constantly change the direction of buckling and form a plurality of turning, the top of spun yarn pocket is equipped with a plurality of roots and hangs the rope, a plurality of hang rope card respectively go into grooving (241) and the centre gripping is fixed within lug (24).
9. The method for rapid propagation of fly pupae of drosophila melanogaster according to claim 6, wherein: the barrel comprises a barrel body (1), a discharging channel (15) which is blocked by a discharging rubber plug (16) is arranged on the side wall of the barrel body and close to the bottom end of the barrel body, and further comprises a net bag (5) and a guide sleeve (6), one end of the guide sleeve (6) is sleeved outside the discharging channel (15), a groove is formed in the other end of the guide sleeve, an elastic ring is arranged in the groove, and the opening of the net bag (5) is bound on the guide sleeve (6) through the elastic ring.
10. The method for rapid propagation of fly pupae of drosophila melanogaster according to claim 9, wherein: the utility model discloses a fly swatter, including section of thick bamboo, section of thick bamboo center of apron of cover (3) is equipped with material ejection way (35) by foraminiferous plug (36) shutoff, foraminiferous plug (36) interpolation has driven fly swatter (4), it includes bracing piece (41), handle (42) and net bat (43) to drive fly swatter (4), handle (42) and net bat (43) are fixed respectively at the upper and lower both ends of bracing piece (41), the bottom of bracing piece (41) is equipped with inflation portion (411) that suit with the central through-hole aperture.
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|---|---|---|---|---|
| US6291739B1 (en) * | 2000-03-24 | 2001-09-18 | Council Of Scientific And Industrial Research | Method for screening of potential anti-epileptic drugs using a Drosophila melanogaster model |
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| CN110742024A (en) * | 2019-11-26 | 2020-02-04 | 福建农林大学 | Simple mass propagation method for drosophila melanogaster pupae |
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| US6291739B1 (en) * | 2000-03-24 | 2001-09-18 | Council Of Scientific And Industrial Research | Method for screening of potential anti-epileptic drugs using a Drosophila melanogaster model |
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| CN109221019A (en) * | 2018-10-15 | 2019-01-18 | 天峨县平昌生态农业有限公司 | A kind of black soldier flies emergence device convenient for collection |
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