CN110301404B - Device for collecting virgo fruit flies - Google Patents

Device for collecting virgo fruit flies Download PDF

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Publication number
CN110301404B
CN110301404B CN201910688055.9A CN201910688055A CN110301404B CN 110301404 B CN110301404 B CN 110301404B CN 201910688055 A CN201910688055 A CN 201910688055A CN 110301404 B CN110301404 B CN 110301404B
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air box
incubator
outer end
base
pipe
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CN110301404A (en
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刘宪楚
刘明
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Hunan University of Arts and Science
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61DVETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D7/00Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
    • A61D7/04Devices for anaesthetising animals by gases or vapours; Inhaling devices

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Anesthesiology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention discloses a device for collecting virgins and fruit flies, which comprises a base, wherein an incubator is arranged on the base, and an anesthesia extraction mechanism and a secondary observation discrimination mechanism are arranged on the base corresponding to the incubator; the invention provides a device for collecting virgin fruit flies, which comprises a base, an anesthesia extraction mechanism and a fan, wherein the anesthesia extraction mechanism comprises a fixing frame, the fixing frame is arranged on the side edge of an incubator on the base, the air box is arranged above the fixing frame, the end part of the air box is communicated with the top of the incubator through a communicating pipe, a culture medium is arranged at the bottom in the incubator, an air pipe is arranged at the top end in the air box, and the fan is arranged on the air pipe.

Description

Device for collecting virgo fruit flies
Technical Field
The invention belongs to the technical field of experiments, and particularly relates to a device for collecting virgins.
Background
Fruit flies are widely present in temperate and tropical climate areas of the world, and since the staple food is yeast and rotten fruits are easy to breed yeast, the trace of the fruit flies can be seen in the habitats of human beings, such as gardens, vegetable markets and the like. At least 1000 or more fruit fly species are currently found except north and south, most species feed on rotten fruits or plants, and a small part takes only fungi, sap or pollen as their food; the fruit flies are the most commonly used experimental life of scientists exploring life sciences, and the tiny ones solve the big problem of human life, including how to influence life with respect to time. Since people or scientists begin to use the fruit fly as an important experimental tool for researching life sciences, the fruit fly not only can be propagated quickly and save the experimental time, but also has high gene similarity with the human, thereby being more beneficial to the research of people. Since the 70's of the 20 th century, Drosophila was widely used in developmental biology research to understand how a relatively simple fertilized egg evolved into a complex organism, including embryonic development, and how various adult organs, such as eyes, wings, legs, brain, heart, etc., developed. In the aspect of neurobiology research, drosophila is also a good model, and development of the nervous system and higher nervous activity and behavior mechanisms are researched through the drosophila. Drosophila melanogaster has been widely used as a model organism in various fields of biology.
The fruit flies need to utilize virgin fruit flies to carry out experiments in the experimental process, however, the fruit flies are extremely easy to mate in the culture process, and the extraction of the virgin fruit flies is very inconvenient.
Disclosure of Invention
The invention aims to: in order to solve the technical problems related to the background technology, a device for collecting virgins is provided.
The technical scheme adopted by the invention is as follows:
a device for collecting virginator fruit flies comprises a base, wherein an incubator is arranged on the base, and an anesthesia extraction mechanism and a secondary observation discrimination mechanism are arranged on the base corresponding to the incubator;
the during operation, the fruit bat is cultivateed to the incubator on the base, within 10 hours from the eclosion beginning at the fruit bat, when female fruit bat is not ripe yet and does not have mating ability, start anesthesia extraction mechanism and carry out anesthesia extraction to the fruit bat in the incubator to utilize the magnifying glass to distinguish sexually, play male fruit bat, carry out preliminary screening, utilize secondary observation discrimination mechanism to carry out secondary observation discrimination to female fruit bat afterwards, obtain virgin fruit bat.
The anesthesia extraction mechanism comprises a fixing frame, the fixing frame is arranged on the side edge of an incubator on a base, an air box is arranged above the fixing frame, the end part of the air box is communicated with the top of the incubator through a communicating pipe, a culture medium is arranged at the bottom in the incubator, an air pipe is arranged at the top end in the air box, a fan is arranged on the air pipe, the bottom end of the air pipe is communicated with the air box, and a blocking net is arranged at the end part of; a drawer plate is arranged at the bottom of the air box, anesthetic is placed on the drawer plate, a handle is connected to the outer end of the drawer plate, and the outer end of the handle extends out of the outer side of the air box; the fan works to generate airflow to push the fruit flies to enter the air box from the communicating pipe, the anesthetic on the drawer plate is used for anesthetizing the fruit flies, and the anesthetized fruit flies fall on the drawer plate;
wherein, a trap lamp is arranged in the air box; the insect-attracting effect is improved, so that the fruit flies smoothly enter the air box.
The secondary observation and identification mechanism comprises a bracket, a fixed table is arranged on the base and corresponds to the lower part of the fixed frame, and the bracket is arranged on the fixed table; a plurality of observation tubes are fixed on the bracket; a funnel cover is arranged at the top of the observation tube; putting the fruit flies on the drawer plate into an observation tube, observing in the observation tube, and judging whether worm eggs appear or not;
the support is arranged below the base in a bilateral symmetry mode, and traveling wheels are arranged at the bottom end of the support; the movable use is convenient.
Wherein, the pipe is rolled over for the L type to communicating pipe, the moth-killing lamp sets up the L type and rolls over the pipe department of bending for the moth-killing lamp directly shines in the mouth of pipe position that corresponds the incubator communicating pipe, improves the moth-killing effect.
Wherein, it is provided with the flange lens to observe tub bottom side, is convenient for observe the intraductal fruit bat of observation, judges whether there is the worm ovum, and then improves the secondary and differentiates efficiency.
Wherein, it is provided with the convex mirror panel that plays the enlarged effect to correspond the observation pipe side on the fixed station, further convenient observation.
Wherein, the drawer panel setting is a book shape drawer panel, and book shape drawer panel outer end is connected with the baffle, and the sealed bellows outer end of baffle butt, tuber pipe correspond to set up in book shape drawer panel top, roll over shape drawer panel and meet communicating pipe, and its book shape section has the cushioning effect.
The drawer plate is replaced by a folded guide plate, one end of the folded guide plate is inclined and abutted against the lower opening of the communicating pipe, the other end of the folded guide plate is horizontally abutted against the bottom plate of the air box and extends out of the outer side of the air box, and the outer end of the air box, corresponding to the outer end of the folded guide plate, is provided with a liftable magnifier; the outer end of the air box is provided with a vertical sliding rail, a sliding rod is sleeved on the vertical sliding rail, the sliding rod is sleeved in a sliding groove of the vertical sliding rail, the left and right sides of the sliding rod in the sliding groove are supported at the bottom of the sliding groove through two telescopic springs, the outer end of the sliding rod is connected with a magnifying lens, and the outer end of the magnifying lens is connected with a handle rod; the folded guide plate enables the fruit flies to enter the outer end of the air box after anesthesia, and the fruit flies are preliminarily screened and distinguished through the liftable magnifying glass.
In summary, due to the adoption of the technical scheme, the invention has the beneficial effects that:
1. the invention provides a device for collecting virgins drosophila, which is characterized in that when the device works, drosophila is cultured in an incubator on a base, within 10 hours from emergence of the drosophila, when female drosophila is not mature and has no mating ability, an anesthesia extraction mechanism is started to perform anesthesia extraction on the drosophila in the incubator, male and female identification is performed by utilizing a magnifying lens, male drosophila is kicked off, primary screening is performed, and then secondary observation identification is performed on the female drosophila by utilizing a secondary observation identification mechanism to obtain virgins.
2. The invention skillfully designs an anesthesia extraction mechanism, utilizes the working of a fan to generate airflow to push fruit flies to enter the air box from the communicating pipe, anesthetizes the fruit flies by anesthetic on the drawer plate, and the anesthetized fruit flies fall on the drawer plate.
3. The invention further comprises a folded drawer plate, the outer end of the folded drawer plate is connected with a baffle, the baffle is abutted against the outer end of the sealed air box, the air pipe is correspondingly arranged above the top of the folded drawer plate, the folded drawer plate is arranged against the communicating pipe, and the folded section of the folded drawer plate has a buffering effect.
4. The fruit fly anesthesia device further comprises a drawer plate, wherein one end of the folded guide plate is inclined and is abutted against the lower opening of the communicating pipe, the other end of the folded guide plate is horizontally abutted against the bottom plate of the air box and extends out of the outer side of the air box, the outer end of the air box is provided with a liftable magnifier corresponding to the outer end of the folded guide plate, the folded guide plate enables fruit flies to enter the outer end of the air box after anesthesia, and the liftable magnifier is used for preliminarily screening and distinguishing the fruit flies.
5. The invention ingeniously designs a secondary observation and discrimination mechanism, which comprises a bracket, wherein a fixed table is arranged on a base corresponding to the lower part of a fixed frame, and the bracket is arranged on the fixed table; a plurality of observation tubes are fixed on the bracket; a funnel cover is arranged at the top of the observation tube; putting the fruit flies on the drawer plate into an observation tube, observing in the observation tube, and judging whether worm eggs appear or not; the observation tube bottom side is provided with the flange lens, is convenient for observe the fruit bat in the observation tube, judges whether there is the worm ovum, and then improves the secondary and differentiates efficiency, in addition it is provided with the protruding mirror panel of enlarged effect to correspond the observation tube side on the fixed station, further convenient observation.
Drawings
FIG. 1 is a schematic diagram of the structure of a first embodiment of the present invention;
FIG. 2 is a schematic view of the structure of a second embodiment of the present invention;
FIG. 3 is a schematic diagram showing the structure of a third embodiment of the present invention;
FIG. 4 is a schematic view of the structure of a fourth embodiment of the present invention;
FIG. 5 is a schematic diagram showing the structure of a fifth embodiment of the present invention;
FIG. 6 is a schematic view of the vertical slide rail and the slide bar according to the fifth embodiment of the present invention;
fig. 7 is a cross-sectional view of the vertical slide rail and the slide bar in accordance with the fifth embodiment of the present invention.
The labels in the figure are: 1. a base; 2. a culture medium; 3. an incubator; 4. a communicating pipe; 5. a trap lamp; 6. blocking the net; 7. an air duct; 8. a fan; 9. an air box; 10. a drawer plate; 11. a handle; 12. a funnel cover; 13. an observation tube; 14. a bracket; 15. a fixed table; 16. a fixed mount; 17. a traveling wheel; 18. a support; 19. a flanged lens; 20. a convex mirror panel; 21. a folded drawer plate; 22. a baffle plate; 23. a handle; 24. a folded deflector; 25. a vertical slide rail; 26. a slide bar; 27. a magnifying glass; 28. a handle; 29. a chute; 30. a telescoping spring.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention; the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance; furthermore, unless expressly stated or limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, as they may be fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
In the first embodiment, referring to fig. 1, a device for collecting virgins drosophila comprises a base 1, wherein an incubator 3 is arranged on the base 1, and an anesthesia extraction mechanism and a secondary observation identification mechanism are arranged on the base 1 corresponding to the incubator 3;
the anesthesia extraction mechanism comprises a fixed frame 16, the fixed frame 16 is arranged on the side edge of the incubator 3 on the base 1, an air box 9 is arranged above the fixed frame 16, the end part of the air box 9 is communicated with the top of the incubator 3 through a communicating pipe 4, a culture medium 2 is arranged at the bottom in the incubator 3, an air pipe 7 is arranged at the top end in the air box 9, a fan 8 is arranged on the air pipe 7, the bottom end of the air pipe 7 is communicated with the air box 9, and a blocking net 6 is arranged at the end part of the air pipe 7; a drawer plate 10 is arranged at the bottom of the air box 9, anesthetic is placed on the drawer plate 10, a handle 11 is connected to the outer end of the drawer plate 10, and the handle 11 extends out of the air box 9;
wherein, a trap lamp 5 is arranged in the air box 9;
the secondary observation and identification mechanism comprises a bracket 14, a fixed table 15 is arranged on the base 1 below the corresponding fixed frame 16, and the bracket 14 is arranged on the fixed table 15; a plurality of observation tubes 13 are fixed on the bracket 14; the top of the observation tube 13 is provided with a funnel cover 12;
wherein, the supports 18 are symmetrically arranged at the left and right below the base 1, and the bottom ends of the supports 18 are provided with walking wheels 17;
example two, refer to fig. 2; the embodiment adds the following technical features on the basis of the first embodiment: the communicating pipe 4 is an L-shaped folded pipe, and the trap lamp 5 is arranged at the bent part of the L-shaped folded pipe; the bottom side of the observation tube 13 is provided with a flange lens 19.
In a third embodiment, referring to fig. 3, the following technical features are added to the second embodiment: the fixed table 15 is provided with a convex mirror panel 20 which plays a role in amplification and corresponds to the side edge of the observation tube 13.
In a fourth embodiment, referring to fig. 4, the following technical features are added to the first embodiment: drawer board 10 sets up and is a book shape drawer board 21, and book shape drawer board 21 outer end is connected with baffle 22, and baffle 22 butt sealed bellows 9 outer end, tuber pipe 7 correspond and set up in book shape drawer board 21 top.
In a fifth embodiment, referring to fig. 5 to 7, the following technical features are added on the basis of the first embodiment: the drawer plate 10 is replaced by a folded guide plate 24, one end of the folded guide plate 24 is inclined and abutted against the lower opening of the communicating pipe 4, the other end of the folded guide plate 24 is horizontally abutted against the bottom plate of the air box 9 and extends out of the outer side of the air box 9, and a liftable magnifier 27 is arranged at the outer end of the air box 9 corresponding to the outer end of the folded guide plate 24; the outer end of the air box 9 is provided with a vertical slide rail 25, a slide bar 26 is sleeved on the vertical slide rail 25, the slide bar 26 is sleeved in a slide groove 29 of the vertical slide rail 25, the slide bar 26 is supported at the bottom of the slide groove 29 through two telescopic springs 30 from left to right in the slide groove 29, the outer end of the slide bar 26 is connected with a magnifying lens 27, and the outer end of the magnifying lens 27 is connected with a handle rod 28.
The working principle is as follows: the invention provides a device for collecting virgins drosophila, which is characterized in that when the device works, drosophila is cultured in an incubator on a base, within 10 hours from emergence of the drosophila, when female drosophila is not mature and has no mating ability, an anesthesia extraction mechanism is started to perform anesthesia extraction on the drosophila in the incubator, male and female identification is performed by utilizing a magnifier, male drosophila is kicked off, preliminary screening is performed, and then secondary observation identification is performed on the female drosophila by utilizing a secondary observation identification mechanism to obtain virgins; according to the invention, the anesthesia extraction mechanism is skillfully designed, the fan is used for generating air flow to push fruit flies to enter the air box from the communicating pipe, the fruit flies are anesthetized by the anesthetic on the drawer plate, and the anesthetized fruit flies fall on the drawer plate; the invention further comprises a folded drawer plate, the outer end of the folded drawer plate is connected with a baffle, the baffle is abutted against the outer end of the sealed air box, the air pipe is correspondingly arranged above the top of the folded drawer plate, the folded drawer plate is arranged against the communicating pipe, and the folded section of the folded drawer plate has a buffering effect; according to the invention, a drawer plate is further replaced by a folded guide plate, one end of the folded guide plate is inclined and is abutted against the lower opening of a communicating pipe, the other end of the folded guide plate is horizontally abutted against an air box bottom plate and extends out of the outer side of the air box, a liftable magnifying lens is arranged at the outer end of the air box corresponding to the outer end of the folded guide plate, the folded guide plate enables fruit flies to enter the outer end of the air box after anesthesia, and the fruit flies are preliminarily screened and distinguished through the liftable magnifying lens; the invention ingeniously designs a secondary observation and discrimination mechanism, which comprises a bracket, wherein a fixed table is arranged on a base corresponding to the lower part of a fixed frame, and the bracket is arranged on the fixed table; a plurality of observation tubes are fixed on the bracket; a funnel cover is arranged at the top of the observation tube; putting the fruit flies on the drawer plate into an observation tube, observing in the observation tube, and judging whether worm eggs appear or not; the observation tube bottom side is provided with the flange lens, is convenient for observe the fruit bat in the observation tube, judges whether there is the worm ovum, and then improves the secondary and differentiates efficiency, in addition it is provided with the protruding mirror panel of enlarged effect to correspond the observation tube side on the fixed station, further convenient observation.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.

Claims (7)

1. The utility model provides a virgin fruit fly collection device, includes base (1), is provided with incubator (3) on base (1), its characterized in that: an anesthesia extraction mechanism and a secondary observation distinguishing mechanism are arranged on the base (1) corresponding to the incubator (3), the anesthesia extraction mechanism comprises a fixed frame (16), the fixed frame (16) is arranged on the side edge of the incubator (3) on the base (1), an air box (9) is installed above the fixed frame (16), the end part of the air box (9) is communicated with the top part of the incubator (3) through a communicating pipe (4), the bottom part in the incubator (3) is provided with a culture medium (2), the top end in the air box (9) is provided with an air pipe (7), the air pipe (7) is provided with a fan (8), the bottom end part of the air pipe (7) is communicated with the air box (9), and the end part of the air pipe is provided with a blocking net (6); a drawer plate (10) is arranged at the bottom of the air box (9), anesthetic is placed on the drawer plate (10), a handle (11) is connected to the outer end of the drawer plate (10), the handle (11) extends out of the outer side of the air box (9), the secondary observation and identification mechanism comprises a bracket (14), a fixed table (15) is arranged on the base (1) and corresponds to the lower portion of the fixed frame (16), and the bracket (14) is installed on the fixed table (15); a plurality of observation tubes (13) are fixed on the bracket (14); a funnel cover (12) is arranged at the top of the observation tube (13), the drawer plate (10) is replaced by a folded guide plate (24), one end of the folded guide plate (24) is inclined and is abutted against the lower opening of the communicating tube (4), the other end of the folded guide plate is horizontally abutted against the bottom plate of the air box (9) and extends out of the outer side of the air box (9), and a liftable magnifier (27) is arranged at the outer end of the air box (9) corresponding to the folded guide plate (24); the outer end of the air box (9) is provided with a vertical sliding rail (25), a sliding rod (26) is sleeved on the vertical sliding rail (25), the sliding rod (26) is sleeved in a sliding groove (29) of the vertical sliding rail (25), the left and right sides of the sliding rod (26) in the sliding groove (29) are supported at the bottom of the sliding groove (29) through two telescopic springs (30), the outer end of the sliding rod (26) is connected with a magnifying lens (27), and the outer end of the magnifying lens (27) is connected with a handle rod (28).
2. A virgins drosophila collection device as recited in claim 1, wherein: and a trap lamp (5) is arranged in the air box (9).
3. A virgins drosophila collection device as recited in claim 1, wherein: the support is characterized in that supports (18) are symmetrically arranged on the left and right below the base (1), and traveling wheels (17) are arranged at the bottom ends of the supports (18).
4. A virgins fly collection device as claimed in claim 2, wherein: the communicating pipe (4) is an L-shaped folding pipe, and the trap lamp (5) is arranged at the bending position of the L-shaped folding pipe.
5. A virgins drosophila collection device as recited in claim 1, wherein: and a flange lens (19) is arranged on the side edge of the bottom of the observation tube (13).
6. A virgins drosophila collection device as recited in claim 1, wherein: and a convex mirror panel (20) with an amplifying function is arranged on the fixed table (15) corresponding to the side edge of the observation tube (13).
7. A virgins drosophila collection device as recited in claim 1, wherein: drawer board (10) set up and are a book shape drawer board (21), book shape drawer board (21) outer end is connected with baffle (22), baffle (22) butt sealed bellows (9) outer end, and tuber pipe (7) correspond and set up in book shape drawer board (21) top.
CN201910688055.9A 2019-07-29 2019-07-29 Device for collecting virgo fruit flies Active CN110301404B (en)

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Publication number Priority date Publication date Assignee Title
US4252080A (en) * 1979-12-19 1981-02-24 Gioia Michael C Enclosed environment for the study of living things
CN104351145A (en) * 2014-11-07 2015-02-18 山西大学 Virgin fruit fly collection device
CN206314438U (en) * 2016-11-29 2017-07-11 浙江海洋大学 A kind of female male drosophila separator
CN208462746U (en) * 2018-05-29 2019-02-05 云南省农业科学院农业环境资源研究所 A kind of drosophila pupa collection device
CN109566544A (en) * 2019-01-28 2019-04-05 秦永燕 A kind of drosophila culture collection device

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US20160270378A1 (en) * 2012-03-13 2016-09-22 National Tsing Hua University System for classifying tiny insects

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US4252080A (en) * 1979-12-19 1981-02-24 Gioia Michael C Enclosed environment for the study of living things
CN104351145A (en) * 2014-11-07 2015-02-18 山西大学 Virgin fruit fly collection device
CN206314438U (en) * 2016-11-29 2017-07-11 浙江海洋大学 A kind of female male drosophila separator
CN208462746U (en) * 2018-05-29 2019-02-05 云南省农业科学院农业环境资源研究所 A kind of drosophila pupa collection device
CN109566544A (en) * 2019-01-28 2019-04-05 秦永燕 A kind of drosophila culture collection device

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