CN104351145A - Virgin fruit fly collection device - Google Patents
Virgin fruit fly collection device Download PDFInfo
- Publication number
- CN104351145A CN104351145A CN201410623829.7A CN201410623829A CN104351145A CN 104351145 A CN104351145 A CN 104351145A CN 201410623829 A CN201410623829 A CN 201410623829A CN 104351145 A CN104351145 A CN 104351145A
- Authority
- CN
- China
- Prior art keywords
- virgin
- pipe
- fruit bat
- fruit fly
- collector
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000255588 Tephritidae Species 0.000 title abstract 10
- 241000238631 Hexapoda Species 0.000 claims abstract description 19
- 235000013399 edible fruits Nutrition 0.000 claims description 49
- 238000002474 experimental method Methods 0.000 abstract description 5
- 230000008901 benefit Effects 0.000 abstract description 4
- 238000000034 method Methods 0.000 abstract description 4
- 230000002068 genetic effect Effects 0.000 abstract description 2
- 238000009423 ventilation Methods 0.000 abstract 2
- 230000013011 mating Effects 0.000 description 9
- 241000255581 Drosophila <fruit fly, genus> Species 0.000 description 4
- 238000012360 testing method Methods 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 210000001015 abdomen Anatomy 0.000 description 2
- 206010002091 Anaesthesia Diseases 0.000 description 1
- 241000382353 Pupa Species 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- 230000010165 autogamy Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 230000032669 eclosion Effects 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000001418 larval effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000019617 pupation Effects 0.000 description 1
- 238000007634 remodeling Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 230000009261 transgenic effect Effects 0.000 description 1
- 210000001364 upper extremity Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Catching Or Destruction (AREA)
- Feeding And Watering For Cattle Raising And Animal Husbandry (AREA)
Abstract
The invention discloses a virgin fruit fly collection device, and relates to a genetic experiment device, which solves the problems of complicated structure, high cost and trouble collection process in the existing virgin fruit fly collection device. The virgin fruit fly collection device comprises an insect feeding pipe, feed and an air ventilation pipe plug, wherein the feed is put at the bottom part of the insect feeding pipe, and the air ventilation pipe plug is embedded in the end opening of the feeding pipe. The virgin fruit fly collection device is characterized in that a virgin fruit fly collector is arranged in the middle part of the insect feeding pipe, is formed by sleeving a plurality of circular pipes, and is fixedly connected by a cross frame; the distance between the two adjacent circular pipes is slightly smaller than the height of an adult fruit fly; a center shaft which is positioned in the circular pipe at the center of the collector is inserted into a center hole of the cross frame, and enters the feed. The virgin fruit fly collection device is used for collecting the virgin fruit flies, and has the advantages that the structure is reasonable, the operation is simple, and the collection accuracy of the virgin fruit flies is high.
Description
Technical field
The present invention relates to the genetics experiments of fruit bat, particularly a kind of device collecting fruit bat virgin fly.
Background technology
Fruit bat is classical model organism, and the advantages such as have the history of life short, filial generation is many, easily raises, and chromosome number is few, and genetic tool is comprehensive, are widely used in the scientific research of genetics and Developmental Biology.Much great scientific discovery is all accomplished by fruit bat, and fruit bat is a kind of good experiment material thus.
The genetics experiments of fruit bat, needs different mutant strains or transgenic strain to hybridize.One step of hybridization most critical selects the virgin fly of non-mating, then hybridizes with the male worm of other strains.Selecting virgin fly is item very hard work, the efficiency of serious restriction genetics experiments.
At present, collect the method for fruit bat virgin fly, mainly examine under a microscope the biological property of fruit bat, carry out hand picking.The principal character of virgin fly is that health body colour is shallow, and wing is soft and tender, and belly turns white and has the unfixed dark color spots in position, has the female fly of this feature substantially can be judged to be virgin fly.Hand picking virgin fly is wasted time and energy, and selects result and affect by experience and subjective judgement, varies with each individual, is difficult to the accuracy ensureing to select.
Fruit bat is after emergence, and male fly is sought a spouse to female fly.Male fly shows fixing sequencing behavior pattern, comprising: towards female fly, pat female fly, follow female fly, vibration side wing sings " song of seeking a spouse " to female fly, lick female fly afterbody, attempts mating and mating.During mating, male fly is stacked in it female fly, therefore, needs higher spatial altitude.Within after emergence about 2 ~ 6 hours, mating (25 DEG C) can be completed.
Once virgin fruit bat post-coitum, can the sperm of Male Drosophila be deposited in vivo, gradation self-fertilization.If could not accurately select virgin fly, directly can affect the correctness of progeny genotypes, finally cause the failure of testing.
2011, Taiwan Tsing-Hua University successfully researched and developed global stem virgin fruit bat collector.The fruit bat pupa being about to sprout wings is put in test tube, when fruit bat sprouts wings, it is lured to queue up through slype from the other end fragrance of test tube, below phtographic lens is again by the belly spot of fruit bat, special micro-image such as sexual organ, pass to the quick interpretation of computer and start Filtering system, then virgin fly being collected automatically.This virgin fruit bat collector, computer and other attached devices many, complex structure, cost is high.During operation, need target drosophila strains to be placed in device, wait for the longer time, collection process lasts till that abundant fruit bat sprouts wings, and not only needs to differentiate male and female, also will distinguish virgin fly and non-virgin fly.
Summary of the invention
Object of the present invention is intended to the shortcoming overcoming prior art, provides a kind of device collecting virgin fruit bat, and solve existing virgin fruit bat collector structure complicated, cost is high, and the operating time is long, the troublesome technical problem of collection process.
The present invention is achieved by the following technical solutions:
A kind of device collecting virgin fruit bat, comprise: insect raises pipe, be placed on the feed bottom insect raising pipe, be entrenched in the ventilative pipe close that insect raises pipe port, it is characterized in that: described insect is raised in the middle part of pipe and places virgin fruit bat collector, described virgin fruit bat collector is set in together by multiple pipe, and is fixedly connected with by cross; Spacing between adjacent described pipe, is slightly less than the height of an adult fruit bat; The centre bore of described cross inserts the described pipe central shaft being positioned at collector center, and described central shaft and described cross are fixed, and lower end is stretched in described feed.
Further, the upper end of described central shaft is provided with flexible little draw ring.
The present invention compared with prior art, virgin fruit bat collector is set in together by multiple pipe owing to being provided with in the middle part of insect raising pipe, and the spacing between adjacent pipe is slightly less than the height of an adult fruit bat, therefore, enter the fruit bat between adjacent pipe, narrow space limits the behavior that they are sought a spouse, and prevents the fruit bat of emergence to carry out mating.After take out fruit bat from collector, only need to give up Male Drosophila, remaining female Drosophila is virgin fly, drastically increases operating efficiency like this.Multiple tube wall adds the space of larvae pupation, thus adds the quantity of adult eclosion.
The present invention, for collecting fruit bat virgin fly, has rational in infrastructure, simple to operate, collects the advantage that the accuracy of virgin fruit bat is high.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention;
The A-A profile of Fig. 1 that Fig. 2 is.
Embodiment
Embody the exemplary embodiments of feature & benefits of the present invention, will describe in detail in the following description.Be understood that the present invention can have various changes in different embodiments, it neither departs from protection scope of the present invention, and explanation is wherein the use when explaining in itself, and is not used to limit the present invention.
As shown in Figure 1, 2, a kind of device collecting virgin fruit bat, comprise: insect raises pipe 1, be placed on the feed 2 bottom insect raising pipe 1, be entrenched in the ventilative pipe close 3 that insect raises pipe 1 port, described insect is raised in the middle part of pipe 1 and places virgin fruit bat collector 4, and described virgin fruit bat collector 4 is set in together by multiple pipe 41, and is fixedly connected with by cross 42; Spacing H between adjacent described pipe 41, is slightly less than the height of an adult fruit bat; The centre bore of described cross 42 inserts described pipe 41 central shafts 43 being positioned at collector center, and described central shaft 43 is fixed with described cross 42, and lower end is stretched in described feed 2.
The upper end of described central shaft 43 is provided with flexible little draw ring 44, is convenient to take out virgin fruit bat collector 4.
Job step of the present invention:
Fruit bat laid eggs after about 24 hours in feed 2 just can hatch larva.Treat there is abundant larva in insect raising pipe 1, the adult raised by insect in pipe 1 all transfers.Virgin fruit bat collector 4 is placed into insect to raise in pipe 1, central shaft 43 inserts in feed 2, and the lower edge of the multiple pipe of virgin fruit bat collector 4 submerges in feed 2.Ventilative pipe close 3 beyond the Great Wall, the ventilative lower edge of pipe close 3 and the upper limb close contact of virgin fruit bat collector 4, do not stay gap.When fruit bat is to three latter stages in age, the space H entered between adjacent pipe 41 is pupated, sprout wings after, adult is limited in narrow space.During due to fruit bat mating, male worm needs to seek connections with female worm and complete fertilization with it, and the limitation in height in this space fruit bat completes mating, therefore ensure that the female worm sprouting wings is the virgin fly of non-mating, drastically increases the efficiency that virgin fly is collected.
Adult carbon dioxide anesthesia is taken out, gives up male fly, just can obtain virgin fly.
Larval density in pipe 41 can not be too large, and feed is in good condition, does not affect the normal size of larva.Fruit bat after sprouting wings in virgin fruit bat collector 4 should be taken out in 72 hours.
The present invention specifically can implement in a variety of forms and not depart from spirit or the essence of invention, so be to be understood that, above-described embodiment is not limited to aforesaid details, and should explain widely in claim limited range, therefore fall into the change in claim or its equivalent scope and remodeling, all should be claim and contained.
Claims (2)
1. collect the device of virgin fruit bat for one kind, comprise: insect raises pipe (1), be placed on the feed (2) that insect raises pipe (1) bottom, be entrenched in the ventilative pipe close (3) that insect raises pipe (1) port, it is characterized in that: described insect is raised pipe (1) middle part and places virgin fruit bat collector (4), described virgin fruit bat collector (4) is set in together by multiple pipe (41), and is fixedly connected with by cross (42); Spacing (H) between adjacent described pipe (41), is slightly less than the height of an adult fruit bat; The centre bore of described cross (42) inserts described pipe (41) central shafts (43) being positioned at collector center, described central shaft (43) is fixed with described cross (42), and lower end is stretched in described feed (2).
2. the device of collection virgin fruit bat according to claim 1, is characterized in that: the upper end of described central shaft (43) is provided with flexible little draw ring (44).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410623829.7A CN104351145B (en) | 2014-11-07 | 2014-11-07 | A kind of device collecting virgin fruit bat |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410623829.7A CN104351145B (en) | 2014-11-07 | 2014-11-07 | A kind of device collecting virgin fruit bat |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104351145A true CN104351145A (en) | 2015-02-18 |
| CN104351145B CN104351145B (en) | 2016-08-24 |
Family
ID=52518542
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410623829.7A Expired - Fee Related CN104351145B (en) | 2014-11-07 | 2014-11-07 | A kind of device collecting virgin fruit bat |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104351145B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105404920A (en) * | 2015-10-30 | 2016-03-16 | 黄雅琴 | Drosophila fecundity statistical method and special statistical device |
| CN110301404A (en) * | 2019-07-29 | 2019-10-08 | 湖南文理学院 | A kind of device for collecting virgin drosophila |
| CN112753676A (en) * | 2019-11-04 | 2021-05-07 | 何凤侠 | By CO2Adult device for anaesthetizing and selecting mosquitoes and flies |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4106438A (en) * | 1976-09-03 | 1978-08-15 | Nelson Roger K | Method of handling Drosophila |
| US4212267A (en) * | 1978-12-08 | 1980-07-15 | Patterson Irvin G | Insect study station |
| US4224898A (en) * | 1979-05-24 | 1980-09-30 | Carolina Biological Supply Company | Method and means for anesthetizing insects |
| US20110132278A1 (en) * | 2009-09-04 | 2011-06-09 | International Flymasters, Inc. | System and method for release and dispersion of flies or other biological control |
| CN203027935U (en) * | 2013-01-25 | 2013-07-03 | 浙江师范大学 | Novel detachable flat bottom test tube |
-
2014
- 2014-11-07 CN CN201410623829.7A patent/CN104351145B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4106438A (en) * | 1976-09-03 | 1978-08-15 | Nelson Roger K | Method of handling Drosophila |
| US4212267A (en) * | 1978-12-08 | 1980-07-15 | Patterson Irvin G | Insect study station |
| US4224898A (en) * | 1979-05-24 | 1980-09-30 | Carolina Biological Supply Company | Method and means for anesthetizing insects |
| US20110132278A1 (en) * | 2009-09-04 | 2011-06-09 | International Flymasters, Inc. | System and method for release and dispersion of flies or other biological control |
| CN203027935U (en) * | 2013-01-25 | 2013-07-03 | 浙江师范大学 | Novel detachable flat bottom test tube |
Non-Patent Citations (3)
| Title |
|---|
| 刘金香: ""野生果蝇的收集和培养"", 《生物学通报》 * |
| 周天宇 等: ""果蝇实验中海绵塞及移蝇漏斗的制作"", 《生物学通报》 * |
| 汤明 等: ""果蝇实验中的2个小制作"", 《生物学通报》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105404920A (en) * | 2015-10-30 | 2016-03-16 | 黄雅琴 | Drosophila fecundity statistical method and special statistical device |
| CN105404920B (en) * | 2015-10-30 | 2017-09-19 | 信阳农林学院 | A kind of statistical method of drosophila reproductive capacity and special statistics utensil |
| CN110301404A (en) * | 2019-07-29 | 2019-10-08 | 湖南文理学院 | A kind of device for collecting virgin drosophila |
| CN110301404B (en) * | 2019-07-29 | 2021-07-30 | 湖南文理学院 | Device for collecting virgo fruit flies |
| CN112753676A (en) * | 2019-11-04 | 2021-05-07 | 何凤侠 | By CO2Adult device for anaesthetizing and selecting mosquitoes and flies |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104351145B (en) | 2016-08-24 |
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Granted publication date: 20160824 Termination date: 20191107 |