JP4755219B2 - Small fish tank and small fish spawning collection method - Google Patents

Description

  The present invention breeds and breeds small experimental fish such as medaka and zebrafish, which have been in the spotlight as research subjects in medicine and biology in recent years, or small ornamental fish that are widely raised as pets. The present invention relates to a small fish breeding water tank and a small fish spawning collection method using the water tank.

  Traditionally, medaka and zebrafish have been most popular and widely bred not only for pets but also for developmental and genetic studies.

  In recent years, the genomes of both species have been deciphered (medaka was completed in 2007). As a result, the genes of medaka and zebrafish related to human genetic diseases are very similar to those of human genes. I found that there are many. In addition, gene knockout technology was established and established as a state-of-the-art medical and biological research material.

  Medaka and zebrafish have the property of eating their eggs immediately after they are laid. Therefore, in medaka and zebrafish for any purpose, it is necessary to place eggs laid by the parent fish in a place that is not affected by the parents.

  There are three possible ways to avoid the influence of parents.

  The first method is to increase the size of the aquarium for raising the parent fish as much as possible, and make a lot of pebbles, sand, aquatic plants, etc. Forming and hiding eggs and larvae in it will prevent prey from parents. This method is the same as the principle of survival in nature, and only some survival is possible. In addition, when using “egg itself” as in genetics research, eggs are scattered throughout the aquarium and difficult to recover.

  The second method is a method in which aquatic plants and a fibrous matrix for attaching the spawning eggs to the parent fish are placed in the aquarium, and when spawning is confirmed, the aquatic plants are transferred to another aquarium. Medaka has an egg-laying ecology that attaches eggs to aquatic plants on the side of the aquatic plants, and the eggs themselves have strong adhesiveness with attached yarns, so this method transfers the eggs successfully to a different aquarium from the parent. Is possible. However, the problem is that this method cannot be applied to zebrafish that produce eggs that are not sticky at all, and there are not a few eggs that have failed to stick and sink to the bottom of the medaka. In addition, in order to collect the eggs, it is necessary to separate the eggs attached to the aquatic plants, but the shape of the aquatic plants is three-dimensional and complicated (especially the roots of water hyacinth have many fine hair roots) It takes time to recover because the eggs are scattered separately. In addition, a variety of bacteria are attached to the aquatic plants, and putting them in a precious aquarium has the risk of bringing in pathogenic bacteria and leading to the death of the parent fish.

The third method is to form a “net-like structure that allows eggs to pass but cannot pass parent fish” several centimeters above the bottom of the parent fish tank. In order to prevent predators from getting close and preying. In this state, the egg is not preyed, but it will be preyed as soon as it hatches and emerges. Even if the fry stays under the mesh structure, there are few numbers that can grow without being confined to sufficient feed without a narrow area. Therefore, after separating in this way, the parent fish is scooped with a net and moved to another tank prepared in advance, and then all the water in the original tank is discarded while being filtered through the net. Is collected in the net. The problem with this method is that due to the adherence of medaka eggs, the eggs stick together to form a lump and remain on top of the net-like structure and are preyed. The collected eggs are generally managed in a small system such as a petri dish, and if only the eggs are collected by the method 2 described above, only a petri dish and a small amount of water are required. However, according to this method, it is necessary to prepare a new aquarium and water for the parent fish, and a drainage system, which is costly.
Of the above three methods, the second method is mainly used when increasing medaka at school or at home. In the case of zebrafish, a method is often used in which the eggs separated into the bottom of the water by the third method are collected by sucking with a pipette. On the other hand, in research institutions that require reliable recovery of eggs laid, they are generally recovered by the third method.

In order to solve the problems of the above three methods, several conventional techniques have been considered.
For example, Patent Document 1 states that “a partition in which a horizontal egg can pass but a parent fish cannot pass is provided in the aquarium”. "Gathering" consists of "Taking out the collected eggs into a tube connected to the lower part by operating two pinch cocks". First, in this invention, it is supposed that a large area is provided for egg separation at the bottom of the aquarium and is inclined, but this not only increases the volume of the aquarium but also distorts the shape. It is a big problem that the space for doing so becomes very large compared to the amount of water for the parent fish (volume relative to it). In both homes and research facilities, the “place for the aquarium” and “the strength of the stand that can withstand the weight of the aquarium” are the biggest problems, and the increase in volume is related to both. While this technique facilitates egg recovery, it causes two serious problems. It is also suspicious that most of the eggs (especially sticky medaka eggs) gather at the bottom at the specified tilt angle. Also, even when all the water in the aquarium is thrown away and filtered through a net to collect the eggs, even if there is no water, a considerable amount of eggs remain on the bottom of the water. Collecting. For this reason, even if eggs gather at the bottom with this device, it is unlikely that a large number of eggs will be accommodated in the water conduit by only a small amount of water (pressure) to the egg collection passage.

  Further, Patent Document 2 states that “a partition that allows eggs to pass but cannot pass parent fish vertically is provided in the aquarium.” “Inclination attached to the aquarium itself, the pressure of water supplied from the water inlet, and the attractive force of drainage from the drain. The egg is collected and removed from the drain outlet and collected. " First of all, it is necessary to take a wasteful space by adding an inclination, but there is nothing much compared to the previous invention. From the description of the specification, a purifier is provided for each water tank, and the water quality must be managed for each water tank. At present, because of the ease of breeding and water quality management and the need to organize many aquariums in a compact manner, each aquarium is not provided with a purifier or a heating device, and can be managed in units as in JP-A-8-214726. It's generalized, and the technology mentioned here goes against it. In addition, the air-conditioning system controls the temperature inside the unit, thereby controlling the water temperature. If the aquarium is a closed system, this method may be efficient, but in this technology, the water temperature changes drastically because water injection is repeated through another system for egg recovery. Temperature management is not realistic. Moreover, it is necessary to provide a separate system for supplying water to the aquarium, and there are various problems such as increasing the size and cost of the apparatus for that purpose, and what to do with the water source.

  The biggest problem is that, as mentioned many times before, a large water pressure is required to move most of the eggs that have adhered to or dropped on the bottom of the water in a certain direction. Recovery is difficult if only a small amount of water is supplied from above. If you keep supplying a considerable amount of water and draining it all day long, it may be possible to recover, but it will take longer to recover.

  Further, Patent Document 3 provides a parent fish breeding room and an egg collection room in a closed and sealed system, and a mesh is provided between which eggs can pass but parent fish cannot pass, and the eggs are meshed by water pressure from the side of the parent fish breeding room. It is a method of collecting by passing. It is estimated that it is easy to collect eggs in the egg collection chamber if such a closed system and a precise apparatus that can withstand experiments in outer space. However, in order to take out the eggs collected in the egg collection chamber to the outside, it is necessary to release the sealed system at one end, and it takes a lot of labor together with the construction of the sealed system again. In addition, preparing such an expensive, precise, and large-scale device for one system is not realistic for the purpose (for ornamental use and genetic experiment) as described above.

JP 2001-120110 A JP 2004-166553 A JP 2003-235395 A

  The present invention has been made in view of the above-described problems, and is capable of easily recovering eggs laid out of small fish under breeding, and small fish breeding capable of reducing the manufacturing cost with a simple configuration. The issue is to provide a water tank.

  In order to solve the above-mentioned problems, the present invention is a small fish breeding aquarium for breeding small fish such as medaka and zebrafish for experiment or ornament, and collecting eggs, and water and small fish A water tank body having an upper surface opening to be accommodated, a detachable partition member provided in such a manner as to partition the inside of the water tank body into a main chamber and an isolation chamber in the length direction, and a bottom plate whose end portions are rotatably connected A collecting plate that is made up of a wall plate, is normally arranged such that the bottom plate is in contact with the bottom surface of the aquarium body and the wall plate is opposed to the partition plate, and the bottom plate attaches a small fish egg. It is characterized by providing.

  According to this, the partition member is removed from the aquarium body, the small fish is driven from the main chamber to the isolation chamber by the recovery member, the partition member is attached again, and then the recovery member is pulled up from the aquarium body. Fish eggs can be easily recovered. In addition, since it is only necessary to provide a predetermined partition member and a recovery member in the existing water tank main body, the manufacturing cost can be reduced with a simple configuration.

  Moreover, it is preferable to provide the extrusion member which is arrange | positioned in the isolation chamber of the said water tank main body, and can move to the length direction of a water tank main body. According to this, when returning the small fish in the isolation chamber to the main chamber, the small fish can be easily driven to the main chamber by moving the pushing member in the length direction of the aquarium body.

  Moreover, it is preferable that the bottom plate of the recovery member has a large number of small holes and a fiber sheet on the upper surface. According to this, water resistance at the time of movement of a recovery member can be reduced by forming a large number of holes in the bottom plate. Moreover, a small fish egg can be easily attached by providing a fiber sheet.

  The bottom plate of the recovery member preferably has a folded portion at the tip. According to this, when the bottom plate is erected at the time of movement of the recovery member, even if the small fish eggs adhering to the bottom plate fall down, the folded portion can receive the eggs, so the small fish eggs It is possible to collect without any loss.

  Moreover, it is preferable that the bottom face part of the water tank body is formed with a recess at a position corresponding to the tip part of the bottom plate of the recovery member. According to this, when the recovery member moves, the bottom plate rotates around the recess toward the isolation chamber side by fitting the tip of the bottom plate of the recovery member into the recess provided on the bottom surface of the water tank body. The bottom plate can be smoothly moved toward the isolation chamber.

  A small fish laying egg collecting method according to the present invention is a method for collecting small fish eggs using the small fish breeding aquarium according to any one of claims 1 to 5, wherein the partition member is a tank. The first step to be removed from the main body and the wall plate of the recovery member are lifted obliquely upward, and the bottom plate connected to the wall plate is moved to the isolation chamber side so as to be vertical, thereby bringing the small fish into the isolation chamber. A second step of driving up, a third step of completely isolating the small fish into the isolation chamber by reattaching the partition member to the original position of the water tank main body in that state, and lifting the recovery member from the water tank main body, A fourth step of recovering the eggs of the small fish attached to the container, and the recovery member is disposed again in the main chamber in such a manner that the bottom plate is in contact with the bottom of the tank body and the wall plate faces the partition plate. 5 steps, A sixth step of removing the cutting member from the aquarium body and returning the small fish in the isolation chamber to the main chamber; and after the small fish has completely returned to the main chamber, the partition member is reattached to the original position of the aquarium main body. And a seventh step.

  A method for recovering eggs of a small fish using the small fish breeding aquarium according to claim 2, wherein the partition member is removed from the aquarium main body, and the wall plate of the collection member is lifted obliquely upward. A second step of driving the small fish to the isolation chamber by bringing the bottom plate connected to the wall plate to the isolation chamber side so that the bottom plate is vertical, and in that state, the partition member is returned to the original position of the tank body. A third step of completely isolating the small fish into the isolation chamber by attaching, a fourth step of lifting the recovery member from the aquarium body and recovering the eggs of the small fish adhering to the bottom plate, and the recovery plate having the aquarium A fifth step in which the wall plate is disposed in the main chamber again in a manner to be in contact with the inside of the main body and the wall plate is opposed to the partition plate; By And a sixth step of driving the small fish in the isolation chamber into the main room and a seventh step of reattaching the partition member to the original position of the aquarium body after the small fish has completely returned to the main room. Good.

  According to the present invention, the partition member is detached from the aquarium main body, the small fish is driven from the main chamber to the isolation chamber by the recovery member, the partition member is attached again, and then the recovery member is pulled up from the aquarium main body to adhere to the recovery member. Small fish eggs can be collected easily. In addition, since it is only necessary to provide a predetermined partition member and a recovery member in the existing water tank main body, the manufacturing cost can be reduced with a simple configuration.

  Thus, the water supply, water quality management, heat insulation, lighting system, etc. that have been used in the conventional facilities are used as they are, and only the water tank is replaced with the water tank of the present invention.

  In addition, anyone can easily perform the operation in a short time, and can reliably recover all medaka-attached eggs and zebrafish non-attached eggs.

  Moreover, since operations such as crawling the parent fish with a net are not performed, the burden on the parent fish is small. In addition, the parent fish remain in the original tank, so they are not exposed to environmental changes (such as when moved to another tank).

  In addition, the egg is taken out of the water tank in a state of being on (or attached to) a recovery member (nylon mesh, wire net, or carbon fiber mesh) mounted on the recovery plate. After all, the eggs are scattered in the two-dimensional plane, so the collection is very easy. In particular, if the recovery member is black, such as carbon fiber, the eggs on it can be easily identified. In addition, if a material having adhesive properties is used for the recovery member, it is possible to fix a non-adhesive zebrafish egg on the recovery member.

  Moreover, the collection | recovery member can be prevented from carrying in bacteria etc. from the outside by making it disposable. Furthermore, since the collection member collects the garbage that sinks to the bottom together with the egg, it leads to water purification.

  When there is no need to collect the eggs, the collection member with the eggs as in the case of aquatic plants may be transferred to another water tank as it is.

  Moreover, if a recessed part is formed in the bottom face part of a water tank main body, even if most of the water in a water tank is thrown away with a pump after isolate | separating a parent, a parent fish will remain with a water in a recessed part. Water can then be changed without separating the parent fish by pouring water and raising the partition to release the parent fish.

  Next, a small fish (F) breeding aquarium (hereinafter referred to as a main aquarium) according to an embodiment of the present invention will be described with reference to FIGS.

  1 is a longitudinal sectional view of the water tank, FIG. 2 is a plan view of the water tank, and FIG. 3 is a perspective view of a recovery member used in the water tank.

  In this description, the length direction of the main tank is the left-right direction in FIGS. 1 and 2, the width direction is the up-down direction in FIG. 2, and the depth direction is the up-down direction in FIG.

  As shown in FIG. 1 and FIG. 2, the main water tank includes a water tank body (1), a partition member (2) provided inside the water tank body (1), a recovery member (3), and an extrusion member (4). Is the main element.

  The said water tank main body (1) is formed in the rectangular parallelepiped shape where the upper surface was opened, and consists of a transparent member made from a synthetic resin. This water tank main body (1) exists conventionally, and a shape, a material, and a transparency degree may be variously changed.

  A flange (11) is provided around the opening of the water tank body (1). This generally serves as a guide for preventing distortion in the water tank body (1). Further, it is convenient because it can be easily carried by gripping the collar (11).

  Moreover, the step part (13) is provided in the one end part (left end part of FIG. 1, FIG. 2) of the length direction in the bottom face part (12) of the water tank main body (1), and it is a partition member so that it may mention later. (2) and the extrusion member (4) can be placed on the upper surface.

  Moreover, it is the both side surface parts (14) (14) of the width direction of a water tank main body (1), Comprising: The position for inserting the both ends of the said partition member (2) in the position near one end part of a length direction An insertion groove (15) is formed along the depth direction.

  Furthermore, a bottom plate (16) is provided in a portion of the bottom surface portion (12) other than the step portion (13), and a recess (17) is formed between the end portion of the bottom plate (16) and the step portion (13). Has been.

  The partition member (2) is made of a synthetic resin transparent member having a rectangular plate shape in plan view, and is inserted into the insertion grooves (15) and (15) of the water tank body (1) at both ends in the width direction. The water tank body (1) is erected vertically.

  Thus, when the partition member (2) is erected vertically inside the water tank body (1), the inside of the water tank body (1) is separated in the length direction, and one side having a large volume (FIGS. 1 and 2). The right side) is partitioned into a main chamber (5), and one side (left side in FIGS. 1 and 2) having a small volume is partitioned into an isolation chamber (6).

  As will be described later, the small fish (F) is normally accommodated in the main room (5), but at the time of recovery, it is accommodated in the isolation room (6).

  As shown in FIG. 3, the recovery member (3) includes a bottom plate (31) having a rectangular plate shape corresponding to the bottom surface (12) of the main chamber (5), and a side surface of the main chamber (5). And a wall plate (32) having a rectangular shape in plan view corresponding to the portion. The bottom plate (31) and the wall plate (32) are rotatably connected at both ends via hinges (33) so that they can rotate in the length direction.

  In the normal state, the bottom plate (31) is normally brought into contact with the bottom plate (16) of the water tank body (1), and the water tank body (1) so that the wall plate (32) faces the partition plate member (2). It arrange | positions in the main chamber (5) of the said water tank main body (1) in the aspect contact | abutted to the side part (18) of this.

  Further, the bottom plate (31) of the recovery member (3) has a large number of small holes (31a)... And a fiber sheet (34) on the upper surface. This

  Examples of the fiber sheet (34) include a carbon fiber sheet. According to this, small fish (F) often lays eggs selectively on a carbon fiber sheet even when aquatic plants are added. In addition, when used as a substitute for aquatic plants, it is effective as a spawning bed. Furthermore, since the carbon fiber sheet is black, there is a merit that the eggs (E) of small fish (F) can be easily found at the time of recovery.

  The bottom plate (31) of the recovery member (3) has a folded portion (35) at the tip. Thereby, even if the egg (E) of the small fish (F) adhering to the bottom plate (31) falls downward when the bottom plate (31) is erected when the recovery member (3) is moved, the folded portion Since (35) can catch those eggs (E), it becomes possible to collect the eggs (E) of the small fish (F) without fail.

  The pushing member (4) includes a front plate (41) formed in a rectangular plate shape when viewed from the front, and four side plates (42) ... (42) provided at the peripheral edge of the front plate (41). A transparent member made of synthetic resin. It is normally arranged in the isolation chamber (6) of the water tank main body (1), and can be moved in the length direction of the water tank main body (1) during recovery.

  A plurality of small holes (41a) are formed in the front plate (41) so that the front plate (41) can move while reducing the resistance of water as much as possible.

  Next, a method for collecting eggs (E) of small fish (F) using the main water tank will be described with reference to FIG.

  FIG. 4 is a transition diagram showing a method for recovering eggs (E) of small fish (F) using this water tank.

  (A) It is a normal state. In this state, the water tank body (1) is separated into the main chamber (5) and the isolation chamber (6) by the partition member (2), and the recovery member (3) is separated into the isolation chamber (6) in the main chamber (5). The extrusion members (4) are respectively arranged at predetermined positions. The small fish (F) swims freely in the main chamber (5) of the aquarium body (1), and when it lays eggs (E) in a timely manner, the eggs (E) become the bottom plate of the recovery member (3). Drop to (31). At this time, since the fiber sheet (34) is provided on the upper surface of the bottom plate (31), the eggs (E) are easily attached.

  (B) When the egg (E) of the small fish (F) adheres to the bottom plate (31) of the collecting member (3) to some extent, the egg (E) of the small fish (F) is collected. When collecting, first, the partition member (2) is removed from the water tank body (1), and the separated state of the main chamber (5) and the isolation chamber (6) is released. At this time, the small fish (F) can swim in the isolation chamber (6), but in most cases, the small fish (F) often swims in the main chamber (5) having a large volume.

  (C) Next, the wall plate (32) of the recovery member (3) is lifted obliquely upward, and the isolation plate is placed so that the bottom plate (31) connected to the wall plate (32) is vertical. (6) The small fish (F) is driven to the isolation chamber (6) side by moving to the side. At this time, the bottom plate (31) is centered on the recess (17) by fitting the tip of the bottom plate (31) of the recovery member (3) into the recess (17) formed in the water tank body (1). Since it rotates to the isolation room (6) side, a baseplate (31) can be brought near to the isolation room (6) smoothly.

  (D) Thus, when the recovery member (3) is brought to the isolation chamber (6) side so as to be vertical, the wall plate (32) of the recovery member (3) functions as a wall and the small fish (F) is isolated. It will be in the state driven by the chamber (6).

  (E) Next, in the state which maintained the standing state of the wall board (32) of a collection member (3), a small fish is attached by attaching the said partition member (2) in the original position of an aquarium main body (1) again. (F) is completely isolated in the isolation chamber (6). And the said collection member (3) is pulled up from a water tank main body (1), and eggs (E) of the small fish (F) in this collection member (3) are collect | recovered. When collecting the eggs (E) of the small fish (F), it can be easily recovered by removing the fiber sheet (34) provided on the recovery member (3), and a new fiber sheet (34) can be recovered. What is necessary is just to attach to 3).

  (F) The aquarium body of the recovery member (3) such that the bottom plate (31) is in contact with the bottom plate (16) of the aquarium body (1) and the wall plate (32) faces the partition member (2). It arrange | positions again in the main chamber (5) in the aspect contact | abutted to the side part (18) of (1). At this time, since the small fish (F) is isolated in the isolation chamber (6), the main chamber (5) is empty.

  (G) Next, after removing the partition member (2) from the aquarium, the small fish (F) is moved to the main chamber (5) by moving the pushing member (4) in parallel to the main chamber (5) side. Drive to. Generally, just removing the partition member (2) from the aquarium body (1) rarely causes all small fish (F) to go to the main room (5), and in some cases, return to the isolation room (6). May come. Therefore, if the small fish (F) is driven by the pushing member (4), it can be easily and surely returned to the main chamber (5).

  (H) Then, the partition member (2) is attached to the original position of the water tank body (1), and the pushing member (4) is disposed again in the isolation chamber (6). Thereby, a small fish (F) will be in the state which returned to the main room (5) completely, and can lay eggs again in the main room (5) similarly to the initial state.

  In addition, the water tank main body (1), partition member (2), collection member (3), and pushing member (4) according to the present embodiment are not limited to the above-described shapes and materials, and various design changes can be made. Is possible.

  Moreover, although the small fish (F) was driven to the main chamber (5) by the extrusion member (4), the small fish (F) was naturally brought into the main chamber (5) without using the extrusion member (4). It may be returned.

  Further, the bottom plate (31) of the recovery member (3) is brought into contact with the bottom surface portion (12) through the bottom plate (16) of the water tank body (1), but is assumed to be in direct contact with the bottom surface portion (12). Also good. Therefore, the contact referred to in the present invention does not necessarily mean a contact state, but includes a case where other members are interposed.

  Further, the wall plate (32) of the recovery member (3) is brought into contact with the side surface (18) of the water tank body (1), but other members may be interposed or spaced apart. Also good.

  Moreover, although the folding | returning part (35) of the front-end | tip part of the collection | recovery member (3) was formed at acute angle, you may form at right angles as shown in FIG.

  In addition, the folded portion (35) is formed at the distal end portion of the recovery member (3). Good.

  In recent years, the present invention has been used in research and business of individuals for small fish (F) in medicine and biology, or for breeding and breeding small fish (F) in individuals.

It is sectional drawing of the vertical direction of this water tank which concerns on this embodiment. It is a top view of the main water tank of FIG. It is a perspective view of a collection member. It is a transition diagram which shows the collection | recovery method of the egg of a small fish using this water tank. It is an enlarged view of the front-end | tip part of the collection | recovery member which concerns on other embodiment.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 ... Water tank body 2 ... Partition member 3 ... Recovery member 4 ... Extrusion member 5 ... Main chamber 6 ... Isolation chamber 31 ... Bottom plate 32 ... Wall plate 34 ... Fiber sheet

Claims (7)

A small fish rearing tank for breeding small fish such as experimental or ornamental medaka or zebrafish and collecting eggs,
An aquarium body with an upper surface opening for containing water and small fish;
A detachable partition member provided in a manner that partitions the inside of the water tank body into a main chamber and an isolation chamber in the length direction;
The bottom plate is composed of a bottom plate and a wall plate that are pivotally connected to each other. Normally, the bottom plate is in contact with the bottom surface of the aquarium body and the wall plate faces the partition plate. A fish tank for breeding small fish, comprising: a recovery member that attaches eggs.   The aquarium for small fish breeding according to claim 1, further comprising an extruding member disposed in an isolation chamber of the aquarium body and movable in the length direction of the aquarium body.   The water tank for small fish breeding according to claim 1 or 2, wherein the bottom plate of the recovery member is formed with a large number of small holes and a fiber sheet is provided on the upper surface.   The small fish breeding aquarium according to any one of claims 1 to 3, wherein the bottom plate of the recovery member has a folded portion at a tip portion.   5. The small fish breeding aquarium according to claim 1, wherein the bottom of the aquarium body has a recess formed at a position corresponding to a tip of the bottom plate of the recovery member. A method for collecting eggs of small fish using the small fish breeding aquarium according to any one of claims 1 to 5,
A first step of removing the partition member from the aquarium body;
A second step of raising the wall plate of the recovery member diagonally upward and driving the small fish to the isolation chamber by bringing the bottom plate connected to the wall plate to the isolation chamber side so as to be vertical;
A third step of completely isolating the small fish into the isolation chamber by reattaching the partition member to the original position of the aquarium body in that state;
A fourth step of lifting the recovery member from the aquarium body and recovering small fish eggs attached to the bottom plate;
A fifth step of relocating the recovery member in the main chamber in such a manner that the bottom plate is in contact with the bottom of the water tank body and the wall plate faces the partition plate;
A sixth step of removing the partition member from the aquarium body and returning the small fish in the isolation chamber to the main chamber;
And a seventh step of reattaching the partition member to the original position of the aquarium body after the small fish has completely returned to the main room.   A method for recovering eggs of a small fish using the small fish breeding aquarium according to claim 2, wherein the partition member is removed from the aquarium main body, and the wall plate of the collection member is lifted obliquely upward. A second step of driving the small fish to the isolation chamber by bringing the bottom plate connected to the wall plate to the isolation chamber side so that the bottom plate is vertical, and in that state, the partition member is returned to the original position of the tank body. A third step of completely isolating the small fish into the isolation chamber by attaching, a fourth step of lifting the recovery member from the aquarium body and recovering the eggs of the small fish adhering to the bottom plate, and the recovery plate having the aquarium A fifth step in which the wall plate is placed in the main chamber again in a manner to be in contact with the inside of the main body and the wall plate faces the partition plate, and the partition member is removed from the water tank main body, and the pushing member is moved in parallel to the main bedroom. By A sixth step of driving the small fish in the isolation chamber into the main room, and a seventh step of reattaching the partition member to the original position of the aquarium body after the small fish has completely returned to the main room. Small fish spawn collection method.