CN108849748A - Female male drosophila quick disconnector in batches - Google Patents

Abstract

The invention belongs to the technical field of fruit fly genetics experiment equipment, and discloses a batch quick separator of male and female fruit flies, which comprises a collection tube (1), a two-way joint separation screen (3), a male fly collection tube (4) and LED lamp (7); collection pipe (1) and male fly collection pipe (4) are connected with two-way joint separation screen (3) respectively, vertically placed during work, collection pipe (1) is positioned at the bottom; pipe (1), and turn on the LED light switch (8) at the same time, the fruit flies will move upward under the effect of crowding stress, negative geotaxis crawling and LED lights, and the smaller male flies will pass through the male and female fruit fly separation screen (32 ) into the male fly collection tube (4), when there is no fruit fly to pass through in the separation hole (33), the male and female fruit flies in the two tubes are separated by the air inlet (2) and the air inlet (5) respectively. Lightly anesthetized and collected. The invention can rapidly and harmlessly separate male and female fruit flies in batches.

Description

Batch Male and Female Drosophila Rapid Separator

technical field

The invention belongs to the technical field of fruit fly genetics experiment instruments, in particular to a batch fast separator of male and female fruit flies.

Background technique

Drosophila is a classic model animal, which is widely used in the research of genetic development, neurological diseases and behavioral cognition. In the process of experimental operation, it is often necessary to separate male and female fruit flies, especially when carrying out experiments related to genetic development, the number of fruit flies that need to be separated is often relatively large. The traditional method generally uses the fruit flies to be anesthetized first, and then with the help of a microscope , a magnifying glass or visual observation to manually separate the flies one by one. This method takes a long time, and if the anesthesia time is too long, it will easily cause damage to the fruit flies and affect the experimental results. On July 7, 2015, two related invention patents were disclosed at the same time. One is a non-harmful and quick separator for male and female fruit flies with application number 201510394647.1. In the separation pipeline, identify and separate one by one, and use gas to blow them into the breeding bottle. The advantage of this method is that it does not need to anesthetize the fruit flies and has a high accuracy rate, but it needs to be separated by identifying one by one, and the fruit flies are in The movement in the pipeline is often uncontrolled, the operation is cumbersome, and the separation speed is slow; the other is the male and female fruit fly separator with the application number 201510399902.1, which uses the introduction tube to introduce the fruit flies from the culture bottle into the pipeline, and through the Set multiple switches to identify and separate male and female fruit flies one by one, and finally introduce male and female fruit flies into corresponding culture bottles through pipelines. The principle of this device is similar to the first patent, and the advantage is that it does not need to anesthetize fruit flies, and The accuracy rate is high. In addition to the shortcomings similar to the above-mentioned patents, the device does not have any auxiliary measures at the import and export ends of the fruit flies, and the fruit flies have a negative geotaxis crawling behavior. The culture bottles used for the collection of the device are all low Due to the position of the separation pipe, it is easy to cause fruit flies to stay in the pipe, and the separation and collection speed will be affected.

Contents of the invention

The purpose of the present invention is to overcome the deficiencies of the prior art, and provide a batch fast separator of male and female fruit flies, which realizes rapid batch separation of a large number of male and female fruit flies at the same time.

In order to achieve the above object, the technical scheme of the present invention is as follows: a batch of male and female fruit fly separators, comprising collection tubes, two-way joint separation screen, male fly collection tubes, partitions, LED lights and LED light switches; collection tubes and male flies The collection tubes are respectively connected to the card slots at both ends of the two-way joint separation screen; the top of the male fly collection tube is equipped with an LED light and an LED light switch, and the LED light and the body of the male fly collection tube are separated by a partition; when working, the collection tube, two-way The assembly consisting of the joint separation screen and the male fly collection tube is placed vertically, and the collection tube is located at the bottom.

In this scheme, lightly anesthetized fruit flies are put into a collection tube, which has a small space and is black. Combined with the 500-600nm yellow-green light emitted by the LDE lamp, the small male flies will pass through the male and female fruit flies. The circular separation hole on the fly separation sieve enters the male fly collection tube, but the larger female flies cannot pass through and are left in the collection tube. After no fruit flies climb out of the separation hole, use the collection tube and the male fly. The air inlet on the upper part of the fly collection tube slightly anesthetized the fruit flies in the tube and collected them separately for culture.

As an improvement, the device is placed vertically when it works, and the collection tube is located at the bottom, which fully utilizes the crawling characteristics of the negative geotaxis of fruit flies and speeds up the speed of fruit flies entering the collection tube of male flies.

As an improvement, the collection tube is black and the space is small, and the speed of fruit flies escaping from the collection tube is enhanced under the double constraints of space and light.

As an improvement, experiments have proved that the yellow-green light of 500-600nm has the effect of obviously attracting fruit flies, so the use of this LED light can better induce fruit flies to enter the collection tube of male flies.

As an improvement, there are multiple separation holes on the male and female fruit fly separation sieve, which can satisfy the rapid passage of multiple male flies at the same time and improve the separation efficiency.

In summary, the present invention has the advantages of high separation efficiency, simple structure, and convenient operation, and can efficiently and harmlessly separate male and female fruit flies in batches.

Description of drawings

Fig. 1 is a structural schematic diagram of the present invention.

Fig. 2 is a side view of 3 in Fig. 1 .

Fig. 3 is a top view of 3 in Fig. 1 .

Fig. 4 is a working diagram of 7 in Fig. 1 .

In the figure: 1. Collection pipe; 2. Air inlet; 3. Two-way joint separation screen; 31. Card slot; 32. Male and female fruit fly separation screen; 33. Separation hole; 4. Male fly collection pipe; 5. Air intake 6, clapboard; 7, LED light; 71, circuit; 72, lithium battery; 8, LED light switch.

Detailed ways

As shown in Figures 1-4, the present invention discloses a quick separator of male and female fruit flies in batches, comprising a collection tube 1, a two-way joint separation screen 3, a male fly collection tube 4, a partition 6, an LED light 7 and an LED light switch 8. The collection pipe 1 and the male fly collection pipe 4 are respectively connected to the slots 31 at both ends of the two-way joint separation screen 3. During operation, the assembly composed of the collection pipe 1, the two-way joint separation screen 3 and the male fly collection pipe 4 is placed vertically, and the collection pipe 1 is located at the bottom. . The collection tube 1 and the male and female fruit fly separation sieve 32 are both black, and the fruit flies to be separated after slight anesthesia are put into the collection tube 1, and the LED light switch 8 on the top of the male fly collection tube 4 is turned on at the same time, and the LED light 7 is replaced by a replaceable The lithium battery 72 provides power and is separated from the tube body by the partition 6; after the fruit flies in the collection tube 1 wake up, they will move upwards under the action of crowding stress, negative geotaxis crawling and LED lights, and the individuals are smaller The male flies enter the male fly collection pipe 4 through the circular separation hole 33 on the male and female fruit fly separation screen 32, and when no fruit flies pass through the separation hole 33, they are collected by the air inlet 2 of the collection pipe 1 and the male flies. The air inlet 5 of the tube 4 lightly anesthetizes the male and female flies in the two tubes respectively and collects them.

Claims (5)

1. a kind of female male drosophila quick disconnector of batch, it is characterised in that：Including collecting pipe（1）, bidirectional joint bolter（3）, Male fly collecting pipe（4）, partition（6）, LED light（7）, LED light switch（8）.

2. the female male drosophila quick disconnector of batch as described in claim 1, it is characterised in that：The collecting pipe（1）With male fly Collecting pipe（4）Respectively with bidirectional joint bolter（3）The card slot at both ends（31）It is connected；Male fly collecting pipe（4）Top is equipped with LED light （7）And LED light switch（8）, LED light（7）With male fly collecting pipe（4）By partition between tube body（6）It separates；Collecting pipe when work （1）, bidirectional joint bolter（3）And male fly collecting pipe（4）The component of composition is disposed vertically, collecting pipe（1）Positioned at bottom.

3. the female male drosophila quick disconnector of batch as described in claim 1, it is characterised in that：The collecting pipe（1）It is one There are the anesthetic gases air inlet of a 0.25mm in the lighttight lucite tube of shorter black, tube wall top（2）.

4. the female male drosophila quick disconnector of batch as described in claim 1, it is characterised in that：The bidirectional joint bolter （3）Both ends contain card slot（31）, centre is one piece circular containing multiple 0.6mm ± 0.1mm sorting holes（33）Female male drosophila separation Sieve（32）.

5. the female male drosophila quick disconnector of batch as described in claim 1, it is characterised in that：The hero fly collecting pipe（4）It is There are the anesthetic gases air inlet of a 0.25mm in a piece transparent organic glass pipe, tube wall top（5）, tube bottom is equipped with launch wavelength In the yellow green LED light of 500-600nm（7）, by button-type lithium cell（72）Power supply is provided, route is passed through（71）And LED light is opened It closes（8）It is controlled；LED light and male fly collecting pipe（4）By lamina of septum pellucidum between tube body（6）It separates.