CN111149466A - Water culture seedling method and device for melon seeds - Google Patents
Water culture seedling method and device for melon seeds Download PDFInfo
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- CN111149466A CN111149466A CN202010248889.0A CN202010248889A CN111149466A CN 111149466 A CN111149466 A CN 111149466A CN 202010248889 A CN202010248889 A CN 202010248889A CN 111149466 A CN111149466 A CN 111149466A
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- 241000353135 Psenopsis anomala Species 0.000 title claims abstract description 176
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 105
- 238000000034 method Methods 0.000 title claims abstract description 78
- 239000008223 sterile water Substances 0.000 claims description 37
- 238000004140 cleaning Methods 0.000 claims description 12
- 238000005286 illumination Methods 0.000 claims description 12
- 230000035784 germination Effects 0.000 claims description 11
- 238000007789 sealing Methods 0.000 claims description 11
- 238000001514 detection method Methods 0.000 claims description 7
- 239000005708 Sodium hypochlorite Substances 0.000 claims description 6
- 230000000249 desinfective effect Effects 0.000 claims description 6
- 238000003825 pressing Methods 0.000 claims description 6
- 238000002791 soaking Methods 0.000 claims description 6
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 4
- 238000005507 spraying Methods 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 2
- 238000004659 sterilization and disinfection Methods 0.000 claims description 2
- 239000003501 hydroponics Substances 0.000 claims 2
- 230000008569 process Effects 0.000 abstract description 44
- 238000012546 transfer Methods 0.000 abstract description 29
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- 241000219112 Cucumis Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
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- 238000003205 genotyping method Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
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- 239000003415 peat Substances 0.000 description 1
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- 229910052902 vermiculite Inorganic materials 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a water culture seedling method and device for melon seeds, and relates to the technical field of seed water culture. The invention provides a water culture seedling raising method and a device for melon seeds, which can be assembled by designing the water culture seedling raising device for melon seeds, adopts a conical sleeve clamp to extract the melon seeds before seedling raising and transfer the melon seeds after seedling raising so as to avoid the melon seeds from being damaged in the transfer process, marking each hole on the hole plate in advance to save the marking operation of each batch of melon seeds, the user can carry out high-efficiency and safe seedling raising operation on the melon seeds in the water culture seedling raising process of the melon seeds, can solve the problems of complex and long seedling raising process of melon seeds and easy damage to the seeds in the transfer operation process, the method has the technical problems of unfriendly identifying the melon seed purity, comprehensively simplifying the melon seed seedling culture process, protecting the seed body safety of melon seeds in the multiple transfer operation process, reducing the seedling culture cost and unfriendly identifying the melon seed purity.
Description
Technical Field
The invention relates to the technical field of seed water culture, in particular to a water culture seedling method and a water culture seedling device for melon seeds.
Background
With the application of molecular marker technology in breeding, the requirements on DNA concentration and yield are not high in the operations of genotyping, molecular marker-assisted breeding selection, variety purity identification and the like, and a DNA sample is not required to be stored for a long time, so that a simple, rapid, low-cost and high-throughput DNA extraction method is very necessary. The method has the advantages that large-scale sample materials are extracted and identified by the high-throughput DNA, the DNA numbers correspond to the sample material numbers one to one, the plant growth is not influenced, and the requirement of rapid identification of seed purity of the melon variety in breeding, production and operation commercialization is met, so that the sample materials are rapidly obtained, the plants correspond to the markers correctly one to one, the growth and the retention of the materials are not influenced, and the method is a basis and an important premise for high-throughput molecular marker identification.
The current common seed germination accelerating seedling raising means is a plug seedling raising technology, light substrates such as peat, vermiculite, perlite and the like are used as seedling raising substrates, a plug is used as a seedling raising container, plug dibbling is manually completed, according to different plug hole numbers, 50 holes, 72 holes, 128 holes and the like are respectively formed, and a 72-hole plug is generally used. The plug seedling has the main advantages of labor saving, low cost, high efficiency, good root system development, high survival rate and convenience for good variety promotion and standard production management.
In the process of implementing the invention, the inventor finds that the related art has at least the following problems:
the existing germination accelerating seedling raising technology for melon seeds usually adopts plug seedling raising, the outdoor temperature required for seedling raising is appropriate, a heating shed is required for seedling raising in winter, the seedling raising conditions are harsh, and a large amount of seedling raising cost is wasted; in addition, when a great number of melon seeds are needed to be subjected to purity identification, the seedling raising task cannot be completed in a short time in a plug seedling raising mode, and after a batch of melon seeds are completed, a great deal of time cost and labor cost are required for processing the melon seeds after seedling raising.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a water culture seedling method and device for melon seeds, which are used for indoor short-term water culture seedling by designing a water culture seedling method and adopting a designed seedling device for seedling in the water culture treatment process, so that the seedling operation process of workers on the melon seeds can be simplified, the seedling efficiency of the melon seeds is improved, the transfer efficiency of the seedling melon seeds is improved, and the melon seeds are prevented from being damaged in the seedling process. The technical scheme of the invention is as follows:
according to a first aspect of the embodiments of the present invention, there is provided a melon seed hydroponic seedling method, which is characterized by comprising:
(1) and (3) sterilization treatment: soaking the target melon seeds for 15-25 min at the temperature of 45-55 ℃, then cleaning with sterile water, disinfecting the target melon seeds for 10min with 3-6% sodium hypochlorite solution, and cleaning again with sterile water;
(2) accelerating germination: wrapping the sterilized target melon seeds with wet gauze, placing the wrapped seeds in a moisture-preserving tray, then sending the seeds into a constant-temperature incubator at the temperature of 30-35 ℃ for accelerating germination, and periodically changing water every 1 day until the target melon seeds sprout roots of 1 +/-0.01 cm;
(3) water culture treatment: sequentially placing the target melon seeds with roots in the marked holes on a hole disc in sequence, clamping the hole disc above a base plate containing sterile water, adjusting the distance of the hole disc above the base plate to enable the roots of the target melon seeds in the hole disc to be in surface contact with the sterile water in the base plate, sealing the hole disc between the base plate and the top cover by using the top cover, and placing the hole disc in a tissue culture chamber for illumination culture for subsequent purity detection, wherein the culture conditions of the tissue culture chamber comprise: and (3) spraying moisture to the target melon seeds every 2 days at the temperature of 24-27 ℃, with the photoperiod of 16h light/8 h dark and the illumination intensity of 1500-2000 Lux, and periodically changing water every 1 day.
In a preferred embodiment, the melon seed hydroponic culture device used in the method comprises: a top cover, an orifice plate and a base plate;
the top cover is arranged above the base plate and is used for being matched with the base plate to seal the hole plate;
cave hole dish card is located the upper portion on chassis, cave hole dish has 96 cave holes, and the specification is 8 holes by 12 holes, and each cave hole has the mark at preset position department sign in proper order according to predetermineeing, and it is downthehole still to overlap to be equipped with toper cover cartridge clip in each cave, toper cover cartridge clip is whole to be invert the cavity taper shape, toper cover cartridge clip bottom have with the through-hole that the cave hole is linked together, toper cover cartridge clip includes: the connecting spring is matched and fixed by a first fixing groove at the inner side of the left semi-conical surface clamp body and a second fixing groove at the inner side of the right semi-conical surface clamp body, and the upper parts of the left semi-conical surface clamp body and the right semi-conical surface clamp body of the conical sleeve clamp are provided with convex pressing sheets;
sterile water is filled at the bottom of the chassis, and air holes are formed in the side wall of the chassis at a preset height away from the liquid level of the sterile water.
In a preferred embodiment, the target melon seeds are placed in conical collet clamps that fit over the respective cavities.
In a preferred embodiment, the melon seed hydroponic culture device has the size of 125 x 92 mm; the size of the top cover is 125 x 92 x 45 mm; the size of the orifice disc is 120 x 88 x 20 mm; the disc depth of the hole disc is 10mm, the depth of each hole is 10mm, the upper aperture is 10mm, and the lower aperture is 3 mm; the chassis size was 125 x 92 x 45 mm.
According to a second aspect of the embodiments of the present invention, there is provided a hydroponic device for culturing melon seeds, the hydroponic device comprising: a top cover, an orifice plate and a base plate;
the top cover is arranged above the base plate and is used for being matched with the base plate to seal the hole plate;
cave hole dish card is located the upper portion on chassis, cave hole dish has 96 cave holes, and the specification is 8 holes by 12 holes, and each cave hole has the mark at preset position department sign in proper order according to predetermineeing, and it is downthehole still to overlap to be equipped with toper cover cartridge clip in each cave, toper cover cartridge clip is whole to be invert the cavity taper shape, toper cover cartridge clip bottom have with the through-hole that the cave hole is linked together, toper cover cartridge clip includes: the connecting spring is matched and fixed by a first fixing groove at the inner side of the left semi-conical surface clamp body and a second fixing groove at the inner side of the right semi-conical surface clamp body, and the upper parts of the left semi-conical surface clamp body and the right semi-conical surface clamp body of the conical sleeve clamp are provided with convex pressing sheets;
sterile water is filled at the bottom of the chassis, and air holes are formed in the side wall of the chassis at a preset height away from the liquid level of the sterile water.
In a preferred embodiment, the target melon seeds are placed in conical collet clamps that fit over the respective cavities.
In a preferred embodiment, the melon seed hydroponic culture device has the size of 125 x 92 mm; the size of the top cover is 125 x 92 x 45 mm; the size of the orifice disc is 120 x 88 x 20 mm; the disc depth of the hole disc is 10mm, the depth of each hole is 10mm, the upper aperture is 10mm, and the lower aperture is 3 mm; the chassis size was 125 x 92 x 45 mm.
Compared with the prior art, the melon seed water culture seedling method and the melon seed water culture seedling device provided by the invention have the following advantages:
1) the seedling raising process is simplified. The invention adopts a brand-new seedling culture device in the water culture seedling culture process, and marks all holes on the hole plate in advance according to the sequence, so that the marking process of each batch of melon seeds can be saved, in addition, the workers can adopt the conical sleeve clamps to carry out extraction transfer before water culture and purity identification transfer after water culture, the transfer process is simple, the volume of the whole melon seed water culture seedling culture device is smaller, the sealing performance is better, and the melon seed water culture seedling culture device can also be carried and transferred efficiently.
2) The safety of the seeds is guaranteed. The water culture seedling raising method for the melon seeds provided by the invention has the advantages that the flow is simple, and the sealing performance of the used water culture seedling raising device for the melon seeds is good, so that the external contact and operation on the melon seeds are less, the natural normal growth of the melon seeds can be realized, and in addition, the seed damage phenomenon in the transferring operation process of the melon seeds can be avoided by using the conical sleeve clamp provided by the invention.
3) Saving the seedling raising cost. The melon seed water culture seedling method and the device are applied to melon seed purity identification, and because melon seeds after purity identification do not need to be reserved, the melon seed water culture seedling method and the device only carry out seedling and germination acceleration on melon seeds, adopt sterile water to replace nutrient solution of the traditional seedling method, and save seedling culture medium required by the traditional plug seedling.
4) The whole process is friendly to the purity identification of the melon seeds. According to the melon seed water culture seedling device, the hole plate is designed with 96 holes with the specification of 8 x 12, the hole plate corresponds to the 96 holes of the material taking plate, transfer butt joint operation of melon seeds is facilitated, the hole plate in the water culture device can be directly placed on the material taking plate after being taken out, then the roots of plants in all the holes are collected into the corresponding holes through scissors, material taking time is greatly saved, the seedling culture process of the melon seeds is simple and efficient, transfer efficiency is high, and each process of melon seed purity identification can be perfectly butted.
In summary, the invention provides a water culture seedling raising method and device for melon seeds, which designs an assembly type water culture seedling raising device for melon seeds, adopts a conical sleeve clamp to extract the melon seeds before seedling raising and transfer the melon seeds after seedling raising, thereby avoiding damage of the melon seeds in the transfer process, marks each hole on a hole tray in advance, saving marking operation for each batch of melon seeds, enabling a user to carry out efficient and safe seedling raising operation on the melon seeds in the water culture seedling raising process of the melon seeds, solving the technical problems that the seedling raising process of the melon seeds is complicated and long, the seeds are easy to be damaged in the multiple transfer operation process, the seedling raising cost is high, the purity identification process of the melon seeds is not good, achieving the technical problems of comprehensively simplifying the seedling raising process of the melon seeds and protecting the seed safety of the melon seeds in the multiple transfer operation process, reducing the seedling raising cost and having the technical effect of unfriendly identifying the purity of the melon seeds.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the invention and together with the description, serve to explain the principles of the invention.
Fig. 1 is a flowchart illustrating a method for hydroponically culturing melon seeds according to an exemplary embodiment.
Fig. 2 is a schematic diagram of a device of a melon seed hydroponic seedling device according to an exemplary embodiment.
FIG. 3 is a schematic view of a tapered collet shown in accordance with an exemplary embodiment.
FIG. 4 is a schematic view of another tapered collet shown in accordance with an exemplary embodiment.
Detailed Description
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.
Fig. 1 is a flowchart illustrating a method for hydroponically culturing melon seeds according to an exemplary embodiment, where the method for hydroponically culturing melon seeds includes, as shown in fig. 1:
step (1): soaking the target melon seeds for 15-25 min at the temperature of 45-55 ℃, then cleaning with sterile water, disinfecting the target melon seeds for 10min with 3-6% sodium hypochlorite solution, and cleaning again with sterile water.
Step (2): and wrapping the sterilized target melon seeds with wet gauze, placing the wrapped seeds in a moisture-preserving tray, then sending the seeds into a constant-temperature incubator at the temperature of 30-35 ℃ for germination acceleration, and periodically changing water every 1 day until the target melon seeds sprout roots of 1 +/-0.01 cm.
And (3): sequentially placing the target melon seeds with roots in the marked holes on a hole disc in sequence, clamping the hole disc above a base plate containing sterile water, adjusting the distance of the hole disc above the base plate to enable the roots of the target melon seeds in the hole disc to be in surface contact with the sterile water in the base plate, sealing the hole disc between the base plate and the top cover by using the top cover, and placing the hole disc in a tissue culture chamber for illumination culture for subsequent purity detection, wherein the culture conditions of the tissue culture chamber comprise: and (3) spraying moisture to the target melon seeds every 2 days at the temperature of 24-27 ℃, with the photoperiod of 16h light/8 h dark and the illumination intensity of 1500-2000 Lux, and periodically changing water every 1 day.
In a preferred embodiment, the melon seed hydroponic culture device used in the method comprises: top cap, cave hole dish, chassis.
For better illustration, the invention shows a schematic device diagram of a melon seed hydroponic seedling device shown in fig. 2. In fig. 2, the hydroponic seedling device for melon seeds comprises: top cover 100, hole plate 200, bottom plate 300.
The top cover 100 is arranged above the bottom plate 300 and is used for being matched with the bottom plate 300 to seal the hole plate 200; the cave hole dish 200 card is located the upper portion of chassis 300, cave hole dish 200 has 96 cave holes 210, and the specification is 8 holes 12, and each cave hole 210 has the mark according to predetermineeing the order in proper order in the department of predetermineeing the position in proper order, still overlaps in each cave hole 210 to be equipped with toper sleeve clamp 220, toper sleeve clamp 220 wholly is inversion cavity taper shape, toper sleeve clamp 220 bottom have with the through-hole that cave hole 210 is linked together.
Wherein, the 96 cave hole design of cave hole dish 200 corresponds with the 96 hole of getting the material board, is favorable to the transfer butt joint operation to the melon seed, and the cave hole dish 200 among the hydroponic device takes out the back, can directly place on getting the material board, then collects the root of each hole plant to corresponding in the hole with the scissors, saves the time of drawing materials greatly. Each hole 210 is marked with a mark at a preset position in turn according to a preset sequence, and may be marked at an upper end position of each hole 210.
To better illustrate the tapered collet 220 provided by the embodiments of the present invention, a schematic view of a tapered collet 220 is shown in fig. 3 and 4, and in fig. 3 and 4, the tapered collet 220 includes: the clamp comprises a left semi-conical clamp body 221, a right semi-conical clamp body 222, a connecting rod 223 for connecting the left semi-conical clamp body 221 and the right semi-conical clamp body 222, and connecting springs 224 arranged on two sides of the inner space of the conical sleeve clamp 220, wherein the connecting springs 224 are fixed by a first fixing groove 225 on the inner side of the left semi-conical clamp body 221 and a second fixing groove 226 on the inner side of the right semi-conical clamp body 222 in a matched manner, and the upper parts of the left semi-conical clamp body 221 and the right semi-conical clamp body 222 of the conical sleeve clamp 220 are respectively provided with a protrusion pressing piece 225.
It should be noted that the target melon seeds are placed in the conical sleeve clamps 220 that are sleeved in the respective cavities 210. When the target melon seeds are in a seedling state, the roots of the target melon seeds sequentially pass through the through holes at the bottom of the conical sleeve clamps 220 and the through holes at the bottom of the target holes to be in contact with the sterile water surface of the chassis 300.
The outer side wall of the conical sleeve clamp 220 can be attached to the inner wall of each hole 210, when a user needs to extract and transfer target melon seeds in a target hole from a melon seed water culture seedling device, the conical sleeve clamp 220 in the target hole and the target melon seeds can be taken out gently and transferred to the upper portion of a target transfer container, and then the conical sleeve clamp 220 is pressed by the left semi-conical surface clamp body 221 and the convex pressing sheet 225 on the upper portion of the right semi-conical surface clamp body 222, so that the conical sleeve clamp 220 is in an open state, the target melon seeds can be transferred into the target transfer container through the open through holes, frequent additional contact on the target melon seeds is not needed in the whole transfer process, contact between the root and cotyledon of the target melon seeds is avoided, and interference of external additional factors on subsequent seed purity detection is avoided.
Sterile water is filled at the bottom of the base plate 300, and air holes are formed in the side wall of the base plate 300 at a preset height from the surface of the sterile water.
The arrangement of the air holes enables the melon seeds in each hole 210 in the hole plate 200 to have enough air to support the growth of the melon seeds when the water culture seedling device for melon seeds is in a closed state.
In a possible embodiment, the top cover 100, the hole tray 200 and the bottom tray 300 are all made of polypropylene PP material, and each part can be independently separated and assembled; the top cover 100 and the bottom plate 300 can be connected and closed in a snap-fit manner.
Preferably, the size of the melon seed water culture seedling device is 125 x 92 mm; the size of the cap 100 is 125 x 92 x 45 mm; the size of the orifice disc 200 is 120 x 88 x 20 mm; the depth of the hole disc 200 is 10mm, the depth of each hole 210 is 10mm, the upper aperture is 10mm, and the lower aperture is 3 mm; the chassis 300 is 125 x 92 x 45mm in size.
The design of the size of the hole disc 200 can effectively prevent the small melon seeds from falling, and the disc depth is 10mm, so that the melon seeds in the holes at the edges can be prevented from falling down.
Because the melon seed water culture seedling device designed by the embodiment of the invention has smaller volume and better sealing performance, in a possible implementation mode, a user can carry the melon seed water culture seedling device by hand through the hand-carrying part arranged at the upper part of the top cover.
The water culture seedling device for melon seeds provided by the embodiment of the invention is adopted to perform water culture seedling of melon seeds, and the following embodiments 1-3 are obtained:
example 1
Step (1): soaking the target melon seeds for 15min at the temperature of 45 ℃, then cleaning the seeds by using sterile water, disinfecting the target melon seeds by using a 3% sodium hypochlorite solution for 10min, and cleaning the seeds by using the sterile water again.
Step (2): wrapping the sterilized target melon seeds with wet gauze, placing the wrapped seeds in a moisture-preserving tray, then sending the seeds into a constant-temperature incubator at 30 ℃ for germination acceleration, and periodically changing water every 1 day until the target melon seeds germinate to form roots of 0.99 cm.
And (3): sequentially placing the target melon seeds with roots in the marked holes on a hole disc in sequence, clamping the hole disc above a base plate containing sterile water, adjusting the distance of the hole disc above the base plate to enable the roots of the target melon seeds in the hole disc to be in surface contact with the sterile water in the base plate, sealing the hole disc between the base plate and the top cover by using the top cover, and placing the hole disc in a tissue culture chamber for illumination culture for subsequent purity detection, wherein the culture conditions of the tissue culture chamber comprise: and (3) spraying moisture to the target melon seeds every 2 days at the temperature of 24 ℃, with the photoperiod of 16h light/8 h dark and the illumination intensity of 1500Lux, and periodically changing water every 1 day.
Example 2
Step (1): soaking the target melon seeds for 20min at the temperature of 50 ℃, then cleaning the seeds by using sterile water, disinfecting the target melon seeds by using a 5% sodium hypochlorite solution for 10min, and cleaning the seeds by using the sterile water again.
Step (2): and wrapping the sterilized target melon seeds with wet gauze, placing the wrapped seeds in a moisture-preserving tray, then sending the seeds into a constant-temperature incubator at 32 ℃ for germination acceleration, and periodically changing water every 1 day until the target melon seeds sprout roots of 1 cm.
And (3): sequentially placing the target melon seeds with roots in the marked holes on a hole disc in sequence, clamping the hole disc above a base plate containing sterile water, adjusting the distance of the hole disc above the base plate to enable the roots of the target melon seeds in the hole disc to be in surface contact with the sterile water in the base plate, sealing the hole disc between the base plate and the top cover by using the top cover, and placing the hole disc in a tissue culture chamber for illumination culture for subsequent purity detection, wherein the culture conditions of the tissue culture chamber comprise: the temperature is 25 ℃, the photoperiod is 16h light/8 h dark, the illumination intensity is 1800Lux, the target melon seeds are sprayed with moisture every 2 days, and the water is periodically changed every 1 day.
Example 3
Step (1): soaking the target melon seeds for 25min at the temperature of 55 ℃, then cleaning the seeds by using sterile water, disinfecting the target melon seeds by using a 6% sodium hypochlorite solution for 10min, and cleaning the seeds by using the sterile water again.
Step (2): wrapping the sterilized target melon seeds with wet gauze, placing the wrapped seeds in a moisture-preserving tray, then sending the seeds into a constant-temperature incubator at 35 ℃ for germination acceleration, and periodically changing water every 1 day until the target melon seeds germinate to form roots of 1.01 cm.
And (3): sequentially placing the target melon seeds with roots in the marked holes on a hole disc in sequence, clamping the hole disc above a base plate containing sterile water, adjusting the distance of the hole disc above the base plate to enable the roots of the target melon seeds in the hole disc to be in surface contact with the sterile water in the base plate, sealing the hole disc between the base plate and the top cover by using the top cover, and placing the hole disc in a tissue culture chamber for illumination culture for subsequent purity detection, wherein the culture conditions of the tissue culture chamber comprise: the temperature is 27 ℃, the photoperiod is 16h light/8 h dark, the illumination intensity is 2000Lux, the target melon seeds are sprayed with moisture every 2 days, and the water is periodically changed every 1 day.
In the process of the embodiment 1-3, the target melon seeds have good growth state, no damage phenomenon occurs in the transfer process, the seed purity identification efficiency is high in the process of identifying the purity of a large batch of target melon seeds, and the period is short.
Obviously, the melon seed water culture seedling method and device provided by the invention have the following advantages:
1) the seedling raising process is simplified. The invention adopts a brand-new seedling culture device in the water culture seedling culture process, and marks all holes on the hole plate in advance according to the sequence, so that the marking process of each batch of melon seeds can be saved, in addition, the workers can adopt the conical sleeve clamps to carry out extraction transfer before water culture and purity identification transfer after water culture, the transfer process is simple, the volume of the whole melon seed water culture seedling culture device is smaller, the sealing performance is better, and the melon seed water culture seedling culture device can also be carried and transferred efficiently.
2) The safety of the seeds is guaranteed. The water culture seedling raising method for the melon seeds provided by the invention has the advantages that the flow is simple, and the sealing performance of the used water culture seedling raising device for the melon seeds is good, so that the external contact and operation on the melon seeds are less, the natural normal growth of the melon seeds can be realized, and in addition, the seed damage phenomenon in the transferring operation process of the melon seeds can be avoided by using the conical sleeve clamp provided by the invention.
3) Saving the seedling raising cost. The melon seed water culture seedling method and the device are applied to melon seed purity identification, and because melon seeds after purity identification do not need to be reserved, the melon seed water culture seedling method and the device only carry out seedling and germination acceleration on melon seeds, adopt sterile water to replace nutrient solution of the traditional seedling method, and save seedling culture medium required by the traditional plug seedling.
4) The whole process is friendly to the purity identification of the melon seeds. According to the melon seed water culture seedling device, the hole plate is designed with 96 holes with the specification of 8 x 12, the hole plate corresponds to the 96 holes of the material taking plate, transfer butt joint operation of melon seeds is facilitated, the hole plate in the water culture device can be directly placed on the material taking plate after being taken out, then the roots of plants in all the holes are collected into the corresponding holes through scissors, material taking time is greatly saved, the seedling culture process of the melon seeds is simple and efficient, transfer efficiency is high, and each process of melon seed purity identification can be perfectly butted.
In summary, the invention provides a water culture seedling raising method and device for melon seeds, which designs an assembly type water culture seedling raising device for melon seeds, adopts a conical sleeve clamp to extract the melon seeds before seedling raising and transfer the melon seeds after seedling raising, thereby avoiding damage of the melon seeds in the transfer process, marks each hole on a hole tray in advance, saving marking operation for each batch of melon seeds, enabling a user to carry out efficient and safe seedling raising operation on the melon seeds in the water culture seedling raising process of the melon seeds, solving the technical problems that the seedling raising process of the melon seeds is complicated and long, the seeds are easy to be damaged in the multiple transfer operation process, the seedling raising cost is high, the purity identification process of the melon seeds is not good, achieving the technical problems of comprehensively simplifying the seedling raising process of the melon seeds and protecting the seed safety of the melon seeds in the multiple transfer operation process, reducing the seedling raising cost and having the technical effect of unfriendly identifying the purity of the melon seeds.
While the invention has been described in detail in the foregoing by way of general description, and specific embodiments and experiments, it will be apparent to those skilled in the art that modifications and improvements can be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Other embodiments of the invention will be apparent to those skilled in the art from consideration of the specification and practice of the invention disclosed herein. This invention is intended to cover any variations, uses, or adaptations of the invention following, in general, the principles of the invention and including such departures from the present disclosure as come within known or customary practice within the art to which the invention pertains. It will be understood that the invention is not limited to the precise arrangements described above and shown in the drawings and that various modifications and changes may be made without departing from the scope thereof.
Claims (7)
1. A water culture seedling method for melon seeds is characterized by comprising the following steps:
(1) and (3) sterilization treatment: soaking the target melon seeds for 15-25 min at the temperature of 45-55 ℃, then cleaning with sterile water, disinfecting the target melon seeds for 10min with 3-6% sodium hypochlorite solution, and cleaning again with sterile water;
(2) accelerating germination: wrapping the sterilized target melon seeds with wet gauze, placing the wrapped seeds in a moisture-preserving tray, then sending the seeds into a constant-temperature incubator at the temperature of 30-35 ℃ for accelerating germination, and periodically changing water every 1 day until the target melon seeds sprout roots of 1 +/-0.01 cm;
(3) water culture treatment: sequentially placing the target melon seeds with roots in the marked holes on a hole disc in sequence, clamping the hole disc above a base plate containing sterile water, adjusting the distance of the hole disc above the base plate to enable the roots of the target melon seeds in the hole disc to be in surface contact with the sterile water in the base plate, sealing the hole disc between the base plate and the top cover by using the top cover, and placing the hole disc in a tissue culture chamber for illumination culture for subsequent purity detection, wherein the culture conditions of the tissue culture chamber comprise: and (3) spraying moisture to the target melon seeds every 2 days at the temperature of 24-27 ℃, with the photoperiod of 16h light/8 h dark and the illumination intensity of 1500-2000 Lux, and periodically changing water every 1 day.
2. The method as claimed in claim 1, wherein the melon seeds hydroponics seedling device used in the method comprises: a top cover, an orifice plate and a base plate;
the top cover is arranged above the base plate and is used for being matched with the base plate to seal the hole plate;
cave hole dish card is located the upper portion on chassis, cave hole dish has 96 cave holes, and the specification is 8 holes by 12 holes, and each cave hole has the mark at preset position department sign in proper order according to predetermineeing, and it is downthehole still to overlap to be equipped with toper cover cartridge clip in each cave, toper cover cartridge clip is whole to be invert the cavity taper shape, toper cover cartridge clip bottom have with the through-hole that the cave hole is linked together, toper cover cartridge clip includes: the connecting spring is matched and fixed by a first fixing groove at the inner side of the left semi-conical surface clamp body and a second fixing groove at the inner side of the right semi-conical surface clamp body, and the upper parts of the left semi-conical surface clamp body and the right semi-conical surface clamp body of the conical sleeve clamp are provided with convex pressing sheets;
sterile water is filled at the bottom of the chassis, and air holes are formed in the side wall of the chassis at a preset height away from the liquid level of the sterile water.
3. The method of claim 2, wherein the target melon seeds are placed in a conical collet that fits over each of the cavities.
4. The method as claimed in claim 2, wherein the melon seed hydroponics growing device is 125 x 92mm in size; the size of the top cover is 125 x 92 x 45 mm; the size of the orifice disc is 120 x 88 x 20 mm; the disc depth of the hole disc is 10mm, the depth of each hole is 10mm, the upper aperture is 10mm, and the lower aperture is 3 mm; the chassis size was 125 x 92 x 45 mm.
5. The utility model provides a melon seed water planting device of growing seedlings which characterized in that, the device includes: a top cover, an orifice plate and a base plate;
the top cover is arranged above the base plate and is used for being matched with the base plate to seal the hole plate;
cave hole dish card is located the upper portion on chassis, cave hole dish has 96 cave holes, and the specification is 8 holes by 12 holes, and each cave hole has the mark at preset position department sign in proper order according to predetermineeing, and it is downthehole still to overlap to be equipped with toper cover cartridge clip in each cave, toper cover cartridge clip is whole to be invert the cavity taper shape, toper cover cartridge clip bottom have with the through-hole that the cave hole is linked together, toper cover cartridge clip includes: the connecting spring is matched and fixed by a first fixing groove at the inner side of the left semi-conical surface clamp body and a second fixing groove at the inner side of the right semi-conical surface clamp body, and the upper parts of the left semi-conical surface clamp body and the right semi-conical surface clamp body of the conical sleeve clamp are provided with convex pressing sheets;
sterile water is filled at the bottom of the chassis, and air holes are formed in the side wall of the chassis at a preset height away from the liquid level of the sterile water.
6. The apparatus of claim 5, wherein the target melon seeds are placed in conical collet clamps that fit over the respective cavities.
7. The device of claim 5, wherein the melon seed hydroponic growing device has a size of 125 x 92 mm; the size of the top cover is 125 x 92 x 45 mm; the size of the orifice disc is 120 x 88 x 20 mm; the disc depth of the hole disc is 10mm, the depth of each hole is 10mm, the upper aperture is 10mm, and the lower aperture is 3 mm; the chassis size was 125 x 92 x 45 mm.
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Cited By (1)
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| CN114916422A (en) * | 2022-06-07 | 2022-08-19 | 贵州大学 | Plant growth sterile experiment culture apparatus |
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