CN111066829B - Disinfectant for rearing instrument in rearing room and preparation method thereof - Google Patents

Disinfectant for rearing instrument in rearing room and preparation method thereof Download PDF

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CN111066829B
CN111066829B CN201911146278.9A CN201911146278A CN111066829B CN 111066829 B CN111066829 B CN 111066829B CN 201911146278 A CN201911146278 A CN 201911146278A CN 111066829 B CN111066829 B CN 111066829B
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parts
plant component
extract
component
rearing
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CN111066829A (en
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黄旭华
李继锋
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Qingdajie Biotechnology Co ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/22—Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/36—Rutaceae [Rue family], e.g. lime, orange, lemon, corktree or pricklyash
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/40—Liliopsida [monocotyledons]
    • A01N65/42—Aloeaceae [Aloe family] or Liliaceae [Lily family], e.g. aloe, veratrum, onion, garlic or chives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L9/00—Disinfection, sterilisation or deodorisation of air
    • A61L9/01—Deodorant compositions
    • A61L9/013—Deodorant compositions containing animal or plant extracts, or vegetable material

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Zoology (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Agronomy & Crop Science (AREA)
  • Microbiology (AREA)
  • Botany (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The invention relates to the technical field of disinfectants, and particularly provides a disinfectant for rearing instruments in a rearing room and a preparation method thereof. The invention provides a disinfectant for silkworm rearing room and rearing instrument, which comprises the following raw materials by weight: a first plant component, a second plant component and 100-200 parts of water; the first plant component comprises: 30-40 parts of honeysuckle, 10-25 parts of green tea, 5-15 parts of common andrographis herb, 10-20 parts of lemon and 8-20 parts of grapefruit; the second plant component comprises 10-20 parts of aloe extract and 4-26 parts of mint extract.

Description

Disinfectant for rearing instrument in rearing room and preparation method thereof
Technical Field
The invention relates to the technical field of disinfectants, and particularly provides a disinfectant for rearing instruments in a rearing room and a preparation method thereof.
Background
The disinfectant commonly used in the current silkworm breeding production mainly comprises two main types, namely chlorine preparation and formaldehyde preparation. The chlorine preparations are mainly bleaching powder, disinfectant for silkworm, and super-chlorine fumigant; the formaldehyde disinfectant mainly comprises formalin lime slurry solution, toxin dissipating agent and the like. The methods can kill pathogenic microorganisms, have quick action and low effective concentration, can obtain better effect, but have certain defects, such as toxicity, irritation, metal corrosivity, poor lasting effect and the like; many disinfectants themselves constitute contaminants, which are present in the environment for a long time, generate different degrees of peculiar smell, and also generate certain toxicity and side effects on the rearing instrument in the rearing room.
Since 2004, the U.S. and the European Union have studied Chinese herbal medicine antibacterial substances successively, and it is clearly indicated in related research reports that the Chinese herbal medicine components have remarkable antibacterial and bacteriostatic activity, and have the characteristics of low toxicity, low residue, environmental friendliness, degradability and the like. However, many Chinese herbal medicine disinfectants have the problems of non-lasting disinfection effect, volatility, poor storage stability and the like, and in addition, the disinfectants applied to the silkworm rearing instruments in the silkworm rearing rooms have certain requirements on deodorization capacity. Therefore, there is a need in the industry for a low-cost disinfectant that is highly effective and durable in disinfection and can be used in rearing rooms.
Disclosure of Invention
In order to solve the above technical problems, a first aspect of the present invention provides a disinfectant for rearing room and rearing instrument, which comprises the following raw materials by weight: a first plant component, a second plant component and 100-200 parts of water; the first plant component comprises: 30-40 parts of honeysuckle, 10-25 parts of green tea, 5-15 parts of common andrographis herb, 10-20 parts of lemon and 8-20 parts of grapefruit; the second plant component comprises 10-20 parts of aloe extract and 4-26 parts of mint extract.
As a preferred technical solution of the present invention, the first plant component further comprises platycodon grandiflorum and grape seeds.
As a preferable technical scheme of the invention, the mass ratio of the platycodon grandiflorum, the grape seeds and the lemon is (0.8-1.2): (0.8-1.2): 1.
as a preferable technical scheme of the invention, the first plant component also comprises pepper.
As a preferred technical scheme of the invention, the mass ratio of the pepper and the mint extract to the grape seeds is (0.5-1): (0.5-1): 1.
as a preferred embodiment of the present invention, the second plant component further comprises spirulina extract and carrot extract.
As a preferable technical scheme of the invention, the mass ratio of the spirulina extract to the carrot extract to the mint extract is (0.8-1.5): (0.3-0.5): 1.
the second aspect of the invention provides a preparation method of the disinfectant for the rearing instrument according to the rearing room, which at least comprises the following steps:
(1) soaking the first plant component in cold water, and drying for later use;
(2) grinding the first plant component, putting into distilled water, cooking, filtering to obtain filtrate and filter residue, and concentrating the filtrate to obtain component A extracting solution;
(3) putting the filter residue obtained in the step (2) into a mixed solution of ethanol \ distilled water, performing reflux extraction, and concentrating to obtain a component B extracting solution;
(4) grinding the second plant component, mixing with the A component extract, the B component extract and water at room temperature, and stirring.
As a preferable technical scheme of the invention, in the step (2), the first plant component is ground and sieved by a sieve of 100-200 meshes.
As a preferable technical scheme of the invention, in the step (4), the second plant component is ground and sieved by a sieve of 80-100 meshes.
Has the advantages that: the invention provides a disinfectant for silkworm rearing room and silkworm rearing instrument and a preparation method thereof. In addition, the disinfectant has good stability, is not easy to volatilize, and is favorable for long-time storage.
Detailed Description
The disclosure may be understood more readily by reference to the following detailed description of preferred embodiments of the invention and the examples included therein. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In case of conflict, the present specification, including definitions, will control.
The terms "comprises," "comprising," "includes," "including," "has," "having," "contains," "containing," or any other variation thereof, as used herein, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.
When an amount, concentration, or other value or parameter is expressed as a range, preferred range, or as a range of upper preferable values and lower preferable values, this is to be understood as specifically disclosing all ranges formed from any pair of any upper range limit or preferred value and any lower range limit or preferred value, regardless of whether ranges are separately disclosed. For example, when a range of "1 to 5" is disclosed, the described range should be interpreted to include the ranges "1 to 4", "1 to 3", "1 to 2 and 4 to 5", "1 to 3 and 5", and the like. When a range of values is described herein, unless otherwise stated, the range is intended to include the endpoints thereof and all integers and fractions within the range.
In addition, the indefinite articles "a" and "an" preceding an element or component of the invention are not intended to limit the number requirement (i.e., the number of occurrences) of the element or component. Thus, "a" or "an" should be read to include one or at least one, and the singular form of an element or component also includes the plural unless the number clearly indicates the singular.
In order to solve the above technical problems, a first aspect of the present invention provides a disinfectant for rearing room and rearing instrument, which comprises the following raw materials by weight: a first plant component, a second plant component and 100-200 parts of water; the first plant component comprises: 30-40 parts of honeysuckle, 10-25 parts of green tea, 5-15 parts of common andrographis herb, 10-20 parts of lemon and 8-20 parts of grapefruit; the second plant component comprises 10-20 parts of aloe extract and 4-26 parts of mint extract.
In a preferred embodiment, the preparation raw materials at least comprise, in parts by weight: the preparation raw materials at least comprise: a first plant component, a second plant component and 100-200 parts of water; the first plant component comprises: 32-38 parts of honeysuckle, 12-23 parts of green tea, 8-12 parts of common andrographis herb, 12-18 parts of lemon and 10-18 parts of grapefruit; the second plant component comprises 12-18 parts of aloe extract and 4-24 parts of mint extract.
In a more preferred embodiment, the preparation of the starting materials comprises at least, in parts by weight: the preparation raw materials at least comprise: a first plant component, a second plant component, and 150 parts water; the first plant component comprises: 35 parts of honeysuckle, 17 parts of green tea, 10 parts of common andrographis herb, 15 parts of lemon and 14 parts of grapefruit; the second plant component comprises 15 parts of aloe extract and 15 parts of mint extract.
The first plant component of the present invention also includes platycodon grandiflorum and grape seeds.
In one embodiment, the mass ratio of the platycodon grandiflorum, the grape seeds and the lemon is (0.8-1.2): (0.8-1.2): 1; more preferably, the mass ratio of the platycodon grandiflorum, the grape seeds and the lemon is 1: 1: 1.
the first plant component also comprises pepper.
In one embodiment, the mass ratio of the pepper, the mint extract and the grape seeds is (0.5-1): (0.5-1): 1; more preferably, the mass ratio of the pepper and the mint extract to the grape seeds is 0.7: 0.7: 1.
the second plant component of the present invention also includes spirulina extract and carrot extract.
In one embodiment, the mass ratio of the spirulina extract, the carrot extract and the mint extract is (0.8-1.5): (0.3-0.5): 1; more preferably, the mass ratio of the spirulina extract, the carrot extract and the mint extract is 1.1: 1.1: 1.
<a first plant component>
Honeysuckle
Honeysuckle, known as honeysuckle, is named from compendium of materia Medica, and is named because honeysuckle flowers turn white at first and then turn yellow. The flos Lonicerae contains chlorogenic acid, luteolin glycoside, etc. The nature is sweet and cold with fragrant smell, sweet and cold with heat clearing without hurting stomach, and the fragrance is thorough and can eliminate pathogenic factors. The honeysuckle can disperse wind-heat and clear away blood toxin, and has obvious effect on various heat diseases such as fever, eruption, macula, sore and carbuncle due to heat toxin, sore throat and the like.
Green tea
Green tea mainly comprises tea polyphenols (catechin), caffeine, aromatic oil, water, minerals, pigment, carbohydrate, protein, amino acids, vitamins, etc. The tea polyphenols contained in the extract can kill botulinum and spores, and inhibit the activity of bacterial exotoxin. Has antibacterial effect on various pathogenic bacteria causing diarrhea, respiratory tract infection and skin infection. The tea polyphenols have obvious inhibiting effect on Staphylococcus aureus and Proteus which cause pyogenic infection, burn and trauma.
Andrographis paniculata (Burm. f.) Nees
The common andrographis herb is also called chunlian willow, and contains diterpene lactone compounds: mainly comprises andrographolide, oroxylin A, andrographolide glycoside, dehydroandrographolide, and contains deoxyandrographolide, neoandrographolide, homoandrographolide, pannicolide, andrographone, and andrographane; also contains 14-deoxy-11-oxyandrographolide, 14-deoxy-11, 12-dideoxy andrographolide, 14-deoxyandrographolide, andrographolide glycoside and 14-deoxyandrographolide glycoside; in addition, the rhizome contains flavonoids. Clear heat and remove toxicity, cool blood and relieve swelling. Can be used for treating common cold, fever, sore throat, aphtha, cough, diarrhea, dysentery, stranguria, carbuncle, suppurative sore, and snake bite.
Lemon
Lemon is a plant of Citrus of Rutaceae of Dicotyledoneae, also known as lemon fruit, Citrus limon, and Yimu fruit. The lemon is rich in vitamin C, saccharides, calcium, phosphorus, iron, vitamin B1, vitamin B2, nicotinic acid, quinic acid, citric acid, malic acid, hesperidin, naringin, coumarin, high-content potassium element, low-content sodium element and the like. And (4) fruit: resolve phlegm and stop cough, promote the production of body fluid and invigorate the stomach. Can be used for treating bronchitis, pertussis, anorexia, vitamin C deficiency, sunstroke and polydipsia. Root: move qi and alleviate pain, relieve cough and dyspnea. Can be used for treating gastralgia, hernia pain, orchitis, cough, and bronchial asthma.
Shaddock
Fructus Citri Grandis is tree of Citrus of Rutaceae. The fructus Citri Grandis contains saccharide, vitamin B1, vitamin B2, vitamin C, vitamin P, carotene, potassium, phosphorus, citric acid, etc. The pericarpium Citri Grandis contains naringin and neohesperidin, and the semen Citri Grandis contains fatty oil, obacunone, and obacunone. Clearing away heat and toxic material, relieving cough and asthma, promoting digestion and regulating qi.
Root of balloonflower
Platycodon grandiflorum, also known as burden flower, small bell flower and mitral flower, is a perennial herbaceous plant. The root contains saponins, known as polygalacic acid, platycodin and glucose. Also contains spinasterol, alpha-spinasterol dill-beta-D-glucoside, delta 7-stigmasterol, betulin, inulin, and platycodon grandiflorum. Three triterpenes are also obtained from platycodon grandiflorum: platycodonic acid A, B, and C. The flower contains delphinidin-3-di-caffeoyllubutose-5-glucoside. Dispersing lung qi, eliminating phlegm, relieving sore throat, and expelling pus. Belongs to heat-phlegm-clearing herbs classified under phlegm-resolving, cough-relieving and asthma-relieving herbs.
Grape seed
The grape seeds are the grape seeds, and the grape skin and the grape stalks are separated after the grape seeds are dried in the sun to obtain the product. Grape seeds contain polyphenols (GPS), mainly catechins and proanthocyanidins. Catechin compounds including catechin, epicatechin and gallate thereof are main monomers in grape seeds, and are also constituent units of oligomeric and polymeric procyanidins. Grape seeds contain abundant oil accounting for 12-15 wt%, and the oil contains a large amount of unsaturated fatty acids, wherein the content of linoleic acid is 58-78%. The grape seeds also contain a small amount of volatile components, and most of the volatile components belong to alcohol, phenol and terpenoid substances and have high biological activity. The grape seed contains crude protein, amino acids, vitamin A, E, D, K, P, and trace elements such as calcium, zinc, ferrum, magnesium, copper, potassium, sodium, manganese, and cobalt. Has antioxidant, blood lipid reducing, and antiallergic effects.
The applicant finds that the lemon and the grapefruit are added into the disinfectant to improve the decontamination performance of the disinfectant during use, but the disinfectant prepared at the moment is easy to generate the phenomenon of layering after being placed at room temperature for a period of time, and the platycodon grandiflorum and the grape seeds added into the disinfectant can effectively improve the problems and improve the deodorization capacity of the disinfectant, which is probably because linoleic acid, platycodon grandiflorum glucoside acid-A methyl ester and other substances contained in the platycodon grandiflorum and the grape seeds can improve the compatibility of a system and avoid the phenomenon of layering of the system; meanwhile, after the triterpenoid saponin contained in the platycodon grandiflorum is shaken in a water phase, a large amount of lasting cellular foam can be generated, so that the surface energy of liquid drops of the disinfectant is increased, and the liquid drops can effectively adsorb and complex molecules with peculiar smell such as ammonia gas, hydrogen sulfide and the like in the air by cooperating with grape seeds and lemons, so that the odor gas is fundamentally removed, and meanwhile, pleasant fragrance is generated, and the deodorant effect is obvious. However, the applicant found that the disinfection duration is not long, probably because the platycodon grandiflorum and the grape seeds are combined with odor molecules to a great extent, so that other components are lost to a great extent, and the disinfection duration is poor.
Chinese prickly ash
The fructus Zanthoxyli is also named as fructus Zanthoxyli, and fructus Zanthoxyli. Contains volatile oil containing limonene, cumic alcohol, geraniol, etc. Also contains 1, 8-cineole, myrcene, vanilline, esculetin, anethole, and thujene. Resisting experimental gastric ulcer, bidirectional action on intestinal smooth muscle movement, resisting diarrhea, protecting liver, tranquilizing, relieving inflammation, local anesthesia, resisting blood coagulation, and killing scabies mite. Pharmacological experiments show that the pericarp injection has the effects of relieving pain and anaesthetizing; geraniol can cause in vitro intestinal tube peristalsis in a small amount and inhibit in a large amount; the decoction has inhibitory effect on Streptococcus, Staphylococcus, pneumococcus, Bacillus anthracis, Bacillus subtilis, Vibrio cholerae, Bacillus paratyphi and Pseudomonas aeruginosa; the contained volatile oil can be used for treating ascariasis and enterobiasis. Warming middle energizer to alleviate pain, killing parasites to relieve itching.
The applicant finds that when the pepper and the mint extract are added into the disinfectant, the disinfection durability and deodorization can be improved, probably because the pepper contains more volatile oil, the pepper can be mutually cooperated with the mint extract and grape seeds, a layer of protective film with permeability can be formed on the surfaces of silkworm rearing rooms and silkworm rearing instruments, and the contained alkaloid, phenol and other substances can continuously play a role, so that the disinfection durability is improved; the contained linalool, menthol and other substances can refresh air and improve deodorization. In addition, the applicant finds that when more pepper is added, the stability and the disinfection lasting effect of the disinfectant are reduced, and when the mass ratio of the pepper, the mint extract and the grape seeds is controlled to be (0.5-1): (0.5-1): 1, the stability and the disinfection durability of the disinfectant can be effectively improved. Probably because the pepper is added too much, on one hand, the pepper is not easy to effectively disperse in the disinfectant and easy to gather and sink; on the other hand, a thicker protective film is easily formed on the surface of the mulberry leaves in a too early state when the disinfectant is used, so that the using effect of the disinfectant is reduced. However, the applicant has now found that the disinfectant is too volatile in summer or when left for a long period of time naturally, and the deodorizing effect is not long lasting.
<A second plant component>
Aloe extract
The aloe extract is prepared from aloe of Liliaceae. Aloe is perennial evergreen herb of genus Aloe of family Africaceae of class Monocotyledonae, also named Aloe barbadensis Miller, Aloe chinensis, Aloe ferox, Aloe vera, Aloe barbadensis Miller, and Aloe vera. The aloe comprises the following components: contains aloe-emodin, aloe-emodin glycoside, and isomer of aloe-emodin glycoside such as aloe-emodin glycoside. High-talaloin, chrysophanol, and chrysophanol glucoside. Anthracenes such as anthralin and glycosides thereof, and flavonoids such as quercetin, kaempferol and rutin, and glucose. Mannose, arabinose, rhamnose, sucrose, xylose, fructose, glucuronic acid and other saccharides. Also contains eight essential amino acids such as arginine, asparagine, glutamic acid, etc., and cholesterol, campesterol, sitosterol. Aloe has effects in tonifying heart, promoting blood circulation, softening arteriosclerosis, reducing cholesterol content, dilating capillary blood vessel, promoting blood circulation, reducing cholesterol value, relieving heart burden, keeping blood pressure normal, and removing toxic substances; promoting wound healing, relieving inflammation, killing bacteria, removing heat and swelling, softening skin, and maintaining cell activity, and the combination of curdlan and wound healing acid has wound healing activity. The aloe extract is purchased from Sancheng biological development, Inc. of Baoji City.
Mint extract
The herba Menthae extract contains volatile oil mainly including I-menthol, 1-menthone and menthyl esters. Disperse wind-heat, clear head and eyes, promote eruption. Can be used for treating wind-heat type common cold, headache, conjunctival congestion, pharyngitis, aphtha, rubella, measles, and chest and hypochondrium swelling. The mint extract was purchased from sanyuan tianyuan biologies ltd.
Spirulina extract
The Spirulina extract refers to Spirulina extract. Spirulina is a kind of lower organism, prokaryote, filament composed of single cell or multiple cells, 200-500 μm in length, 5-10 μm in width, cylindrical, loose or compact regular spiral curve, shaped like clock spring, and named. Spirulina contains proteins (60 wt%), and is mainly composed of isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, valine, etc. In addition, the beverage also contains fat, carbohydrate, chlorophyll, carotenoid, phycocyanin, vitamins A, B1, B2, B6, B12, E, nicotinic acid, creatine, gamma-linolenic acid, calcium pantothenate, folic acid, calcium, iron, zinc, magnesium and the like. Spirulina has been found to have a variety of pharmacological effects. Such as reducing blood fat, resisting oxidation, infection, cancerization, radiation, aging, enhancing immunity, etc. The spirulina extract was purchased from Mufang Biotech limited, Xinyang.
Carrot extract
Carrot extract refers to an extract of carrot. Carrot is also called Asparagus, and is a biennial herb of carrot genus of Umbelliferae family. The carrot contains abundant nutrients including sucrose, starch, carotene and vitamin B1Vitamin B2Folic acid, amino acids (with high lysine content), mannitol, lignin, pectin, quercetin, kaempferol, volatile oil, caffeic acid, calcium, etc. Radix Dauci Sativae contains quercetin, and has effects of increasing coronary blood flow, promoting epinephrine synthesis, lowering blood pressure, and relieving inflammation. Carrot seeds contain 13% of oil, and can expel ascaris and treat dysentery. Carrot leaves can be used for preventing and treating varicella and acute icterohepatitis. The long-term drinking of Sucus Dauci Sativae can prevent nyctalopia and xerophthalmia, and can make skin plump, wrinkle smooth, speckle eliminated and hair beautiful. The carrot extract was purchased from sienna yuze biotechnology limited.
The applicant finds that when the spirulina extract and the carrot extract are added into the disinfectant, the volatility of the disinfectant, particularly the mint therein, can be reduced, so that the disinfection effect is improved, and the deodorization time is longer. The reason the inventor guesses may be that the spirulina extract can increase the binding force between the mint extract and the spirulina extract molecules, reduce the activity of mint oil molecules in the mint extract, reduce the volatility of the mint oil molecules, and make the deodorization effect more durable. Meanwhile, amino acid compounds in the spirulina extract, menthone in the mint extract and pectin in the carrot extract can interact to adsorb more natural bacteria, and the combination effect of porphyrin rings of chlorophyll a in the spirulina extract, protein and inorganic salt in the carrot extract promotes the composition to generate mechanical action with natural bacteria in the air, accelerates the settlement of the natural bacteria in the air, and thus improves the durability of the disinfection effect.
The second aspect of the invention provides a preparation method of the disinfectant for the rearing instrument according to the rearing room, which at least comprises the following steps:
(1) soaking the first plant component in cold water, and drying for later use;
(2) grinding the first plant component, putting into distilled water, cooking, filtering to obtain filtrate and filter residue, and concentrating the filtrate to obtain component A extracting solution;
(3) putting the filter residue obtained in the step (2) into a mixed solution of ethanol \ distilled water, performing reflux extraction, and concentrating to obtain a component B extracting solution;
(4) grinding the second plant component, mixing with the A component extract, the B component extract and water at room temperature, and stirring.
In a preferred embodiment, the preparation method of the disinfectant for the rearing room and rearing instrument at least comprises the following steps:
(1) soaking the first plant component in cold water, and drying for later use;
(2) grinding the first plant component, sieving with a 100-200-mesh sieve, putting into distilled water, cooking, filtering to obtain filtrate and filter residue, and concentrating the filtrate to obtain an A component extracting solution;
(3) putting the filter residue obtained in the step (2) into a mixed solution of ethanol \ distilled water, performing reflux extraction, and concentrating to obtain a component B extracting solution;
(4) and grinding the second plant component, sieving the ground second plant component by a sieve of 80-100 meshes, mixing the ground second plant component with the component A extracting solution, the component B extracting solution and water at room temperature, and stirring uniformly to obtain the plant extract.
In a more preferred embodiment, the preparation method of the disinfectant for the rearing room and rearing instrument at least comprises the following steps:
(1) soaking the first plant component in cold water, and drying at 30 deg.C;
(2) grinding the first plant component, sieving with a 100-200-mesh sieve, putting into distilled water, cooking for 1-3 times, each time for 0.5-1 h, filtering to obtain filtrate and filter residue, and concentrating the filtrate to obtain a component A extracting solution;
(3) putting the filter residue obtained in the step (2) into a mixed solution of ethanol \ distilled water, performing reflux extraction for 2-3 times, and concentrating to obtain a component B extracting solution; the volume ratio of the ethanol to the distilled water is 1: (3-5);
(4) and grinding the second plant component, sieving the ground second plant component by a sieve of 80-100 meshes, mixing the ground second plant component with the component A extracting solution, the component B extracting solution and water at room temperature, and stirring uniformly to obtain the plant extract.
Examples
In order to better understand the above technical solutions, the following detailed descriptions will be provided with reference to specific embodiments. It should be noted that the following examples are only for illustrating the present invention and should not be construed as limiting the scope of the present invention, and that the insubstantial modifications and adaptations of the present invention by those skilled in the art based on the above disclosure are still within the scope of the present invention. In addition, the starting materials used are all commercially available, unless otherwise specified.
Example 1
The embodiment 1 of the invention provides a disinfectant for rearing room and rearing instrument, which comprises the following preparation raw materials in parts by weight: a first plant component, a second plant component, and 100 parts water; the first plant component comprises: 30 parts of honeysuckle, 10 parts of green tea, 5 parts of common andrographis herb, 10 parts of lemon and 8 parts of grapefruit; the second plant component includes 10 parts aloe vera extract.
The first plant component of the present invention also includes platycodon grandiflorum and grape seeds.
In one embodiment, the mass ratio of platycodon grandiflorum, grape seed and lemon is 0.8: 0.8: 1.
the first plant component also comprises pepper.
In one embodiment, the mass ratio of the pepper, the mint extract and the grape seeds is 0.5: 0.5: 1.
the second plant component of the present invention also includes spirulina extract and carrot extract.
In one embodiment, the mass ratio of spirulina extract, carrot extract and mint extract is 0.8: 0.3: 1.
the preparation method of the disinfectant for the rearing instrument in the rearing room comprises the following steps:
(1) soaking the first plant component in cold water, and drying at 30 deg.C;
(2) grinding the first plant component, sieving with a 150-mesh sieve, placing into distilled water, steaming and decocting for 3 times, each time for 1h, filtering to obtain filtrate and residue, and concentrating the filtrate to obtain component A extractive solution;
(3) putting the filter residue in the step (2) into a mixed solution of ethanol \ distilled water, performing reflux extraction for 3 times, and concentrating to obtain a component B extracting solution; the volume ratio of the ethanol to the distilled water is 1: 4;
(4) grinding the second plant component, sieving with a 90-mesh sieve, mixing with the component A extract, the component B extract and water at room temperature, and stirring.
Example 2
The embodiment 2 of the invention provides a disinfectant for rearing room and rearing instrument, which comprises the following preparation raw materials in parts by weight: a first plant component, a second plant component, and 200 parts water; the first plant component comprises: 40 parts of honeysuckle, 25 parts of green tea, 15 parts of common andrographis herb, 20 parts of lemon and 20 parts of grapefruit; the second plant component includes 20 parts aloe vera extract.
The first plant component of the present invention also includes platycodon grandiflorum and grape seeds.
In one embodiment, the mass ratio of platycodon grandiflorum, grape seed and lemon is 1.2: 1.2: 1.
the first plant component also comprises pepper.
In one embodiment, the mass ratio of the pepper and the mint extract to the grape seeds is 1: 1: 1.
the second plant component of the present invention also includes spirulina extract and carrot extract.
In one embodiment, the mass ratio of spirulina extract, carrot extract and mint extract is 1.5: 0.5: 1.
the preparation steps of the disinfectant for the rearing room and the rearing instrument are the same as the example 1.
Example 3
The embodiment 3 of the invention provides a disinfectant for rearing room and rearing instrument, which comprises the following preparation raw materials in parts by weight: a first plant component, a second plant component, and 100 parts water; the first plant component comprises: 32 parts of honeysuckle, 12 parts of green tea, 8 parts of common andrographis herb, 12 parts of lemon and 10 parts of grapefruit; the second plant component includes 12 parts aloe vera extract.
The first plant component of the present invention also includes platycodon grandiflorum and grape seeds.
In one embodiment, the mass ratio of platycodon grandiflorum, grape seed and lemon is 0.8: 0.8: 1.
the first plant component also comprises pepper.
In one embodiment, the mass ratio of the pepper, the mint extract and the grape seeds is 0.5: 0.5: 1.
the second plant component of the present invention also includes spirulina extract and carrot extract.
In one embodiment, the mass ratio of spirulina extract, carrot extract and mint extract is 0.8: 0.3: 1.
the preparation steps of the disinfectant for the rearing room and the rearing instrument are the same as the example 1.
Example 4
The embodiment 4 of the invention provides a disinfectant for rearing room and rearing instrument, which comprises the following preparation raw materials in parts by weight: a first plant component, a second plant component, and 200 parts water; the first plant component comprises: 38 parts of honeysuckle, 23 parts of green tea, 12 parts of common andrographis herb, 18 parts of lemon and 18 parts of grapefruit; the second plant component includes 18 parts aloe vera extract.
The first plant component of the present invention also includes platycodon grandiflorum and grape seeds.
In one embodiment, the mass ratio of platycodon grandiflorum, grape seed and lemon is 1.2: 1.2: 1.
the first plant component also comprises pepper.
In one embodiment, the mass ratio of the pepper and the mint extract to the grape seeds is 1: 1: 1.
the second plant component of the present invention also includes spirulina extract and carrot extract.
In one embodiment, the mass ratio of spirulina extract, carrot extract and mint extract is 1.5: 0.5: 1.
the preparation steps of the disinfectant for the rearing room and the rearing instrument are the same as the example 1.
Example 5
The embodiment 5 of the invention provides a disinfectant for rearing room and rearing instrument, which comprises the following preparation raw materials in parts by weight: a first plant component, a second plant component, and 150 parts water; the first plant component comprises: 35 parts of honeysuckle, 17 parts of green tea, 10 parts of common andrographis herb, 15 parts of lemon and 14 parts of grapefruit; the second plant component includes 15 parts aloe vera extract.
The first plant component of the present invention also includes platycodon grandiflorum and grape seeds.
In one embodiment, the mass ratio of platycodon grandiflorum, grape seed and lemon is 1: 1: 1.
the first plant component also comprises pepper.
In one embodiment, the mass ratio of the pepper, the mint extract and the grape seeds is 0.7: 0.7: 1.
the second plant component of the present invention also includes spirulina extract and carrot extract.
In one embodiment, the mass ratio of spirulina extract, carrot extract and mint extract is 1.1: 1.1: 1.
the preparation steps of the disinfectant for the rearing room and the rearing instrument are the same as the example 1.
Comparative example 1
Comparative example 1 was compared with example 5 without Platycodon grandiflorum, and the rest was the same as example 5.
Comparative example 2
Comparative example 2 compared to example 5, no platycodon grandiflorum, grape seed, otherwise the same as example 5.
Comparative example 3
Comparative example 3 compared to example 5, without lemon, otherwise the same as example 5.
Comparative example 4
Comparative example 4 compared to example 5, without grape seeds, otherwise the same as example 5.
Comparative example 5
Compared with the embodiment 5, the mass ratio of the pepper and the mint extract to the grape seeds is replaced by 2: 0.7: 1, otherwise the same as in example 5.
Comparative example 6
Comparative example 6 compared to example 5, there was no pepper, otherwise the same as example 5.
Comparative example 7
Comparative example 7 compared to example 5, there was no peppermint extract, otherwise the same as example 5.
Comparative example 8
Comparative example 8 compared to example 5, there was no zanthoxylum bungeanum, mint extract, grape seed, otherwise as in example 5.
Comparative example 9
Comparative example 9 compared to example 5, without spirulina, otherwise the same as example 5.
Comparative example 10
Comparative example 10 compares to example 5, a carrot-free radish, otherwise as in example 5.
Comparative example 11
Comparative example 11 compared to example 5, without spirulina, carrot, otherwise the same as example 5.
Performance testing
1. Storage stability test
After the disinfectants prepared in examples 1-5 and comparative examples 1-5 were placed at room temperature for 3 months, whether delamination or turbidity occurred was observed, 100 parallel samples were set for each example, and the probability of delamination or turbidity, i.e., the percentage of the number of delaminated or turbid disinfectants to the total number tested, was recorded.
2. Sterilization durability test
Respectively culturing staphylococcus aureus and escherichia coli by using a BP culture medium and an LB culture medium. The lawn cultured in the two media was washed with 0.03mol/L phosphate buffer containing peptone (2% by mass) to prepare a suspension containing 1X 108 CFU/mL. A filter paper sheet was taken, and the filter paper was punched into a circular paper sheet with a 0.6cm punch, and the disinfectants obtained in examples 1 to 5 and comparative examples 4 to 11 were each absorbed by a pipette at 5. mu.L and dropped onto the paper sheet, and the sample was prepared after sufficient absorption. Pipette 100. mu.L of the bacterial suspension onto TSC agar medium and spread evenly 3 times with a spreader. Gently sticking the sample pieces to the culture plate by using sterile forceps, sticking 1 sample piece to each plate, using normal saline as a control, culturing for 12h and 36h in a constant-temperature incubator at 37 ℃, calculating the change value of the antibacterial rate, repeating the test for 3 times, and taking the average value, wherein the antibacterial rate is (average bacteria number before the test-average bacteria number after the test) multiplied by 100/average bacteria number before the test.
3. Deodorization test
Selecting a silkworm room with obvious odor and the same life time of silkworms, erecting silkworm rearing instruments, uniformly spraying the disinfectant obtained in the examples 1-5 and the comparative examples 1-11 on the front and back surfaces of the large and small silkworm rearing instruments, the walls, the roof, the ground, the surrounding environment and the like by using a sprayer containing the disinfectant, wherein the disinfection spraying amount is 225mL per square meter, immediately closing doors and windows of the silkworm room after the disinfection of the sprayed liquor, heating to 25 ℃, and sealing for 2 hours. The deodorization effect is evaluated by a trained sniffer through artificial olfaction evaluation according to the following standards: 3-odor can be smelled obviously; 2-smell can be smelled slightly; 1-can slightly feel very weak odor, and is similar to the existence or nonexistence; 0-no smell.
4. Volatility test
After 1.0mL of the disinfectant obtained in examples 1 to 5 and comparative examples 6 to 11 was coated on a test plate (2 cm. times.2 cm) made of stainless steel at 25 ℃, the plate was left to stand at a constant temperature of 25 ℃ for one day, and whether or not the coated disinfectant was volatilized (dried) was observed. When the trace of the disinfectant applied to the test plate becomes rainbow spots (interference fringes) corresponding to volatilization, it is determined that the disinfectant is completely volatilized and is defined as "volatilized", and when the trace of the disinfectant applied to the test plate does not become an interference fringe, it is defined as "non-volatilized".
TABLE 1
Stability (%) Durability of Disinfection (%) Deodorizing property Volatility
Example 1 0 5 0 Is not volatile
Example 2 0 4 0 Is not volatile
Example 3 0 5 0 Is not volatile
Example 4 0 4 0 Is not volatile
Example 5 0 3 0 Is not volatile
TABLE 2
Stability (%) Deodorizing property
Comparative example 1 10 2
Comparative example 2 30 3
Comparative example 3 5 1
TABLE 3
Stability (%) Durability of Disinfection (%) Deodorizing property
Comparative example 4 10 8 1
Comparative example 5 15 10 0
TABLE 4
Durability of Disinfection (%) Deodorizing property Volatility
Comparative example 6 15 1 Is not volatile
Comparative example 7 11 1 Is not volatile
Comparative example 8 35 2 Is not volatile
Comparative example 9 10 1 Volatilize
Comparative example 10 9 1 Volatilize
Comparative example 11 30 2 Volatilize
The foregoing examples are merely illustrative and serve to explain some of the features of the method of the present invention. The appended claims are intended to claim as broad a scope as is contemplated, and the examples presented herein are merely illustrative of selected implementations in accordance with all possible combinations of examples. Accordingly, it is applicants' intention that the appended claims are not to be limited by the choice of examples illustrating features of the invention. Also, where numerical ranges are used in the claims, subranges therein are included, and variations in these ranges are also to be construed as possible being covered by the appended claims.

Claims (2)

1. A disinfectant for rearing room and rearing instrument is characterized by comprising the following raw materials in parts by weight: a first plant component, a second plant component and 100-200 parts of water; the first plant component comprises: 30-40 parts of honeysuckle, 10-25 parts of green tea, 5-15 parts of common andrographis herb, 10-20 parts of lemon and 8-20 parts of grapefruit; the second plant component comprises 10-20 parts of aloe extract and 4-26 parts of mint extract;
the first plant component further comprises platycodon grandiflorum and grape seeds;
the mass ratio of the platycodon grandiflorum to the grape seeds to the lemon is 1: 1: 1;
the first plant component also comprises pepper;
the mass ratio of the pepper and the mint extract to the grape seeds is 0.7: 0.7: 1;
the second plant component further comprises spirulina extract and carrot extract;
the mass ratio of the spirulina extract to the carrot extract to the mint extract is 1.1: 1.1: 1.
2. a method for preparing a disinfectant for rearing instruments in a rearing room according to claim 1, which comprises at least the following steps:
(1) soaking the first plant component in cold water, and drying for later use;
(2) grinding the first plant component, putting into distilled water, cooking, filtering to obtain filtrate and filter residue, and concentrating the filtrate to obtain component A extracting solution;
(3) putting the filter residue obtained in the step (2) into a mixed solution of ethanol \ distilled water, performing reflux extraction, and concentrating to obtain a component B extracting solution;
(4) grinding the second plant component, mixing with the component A extracting solution, the component B extracting solution and water at room temperature, and stirring uniformly to obtain the composition;
grinding the first plant component in the step (2) and sieving the ground first plant component by a sieve of 100-200 meshes;
and (4) grinding the second plant component and sieving the ground plant component with a sieve of 80-100 meshes.
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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107744535A (en) * 2017-11-01 2018-03-02 黄世圆 The preparation method of honeysuckle disinfectant
CN110257184A (en) * 2019-06-27 2019-09-20 安徽省华凯轻工科技有限公司 A kind of food-grade alkali lotion

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107744535A (en) * 2017-11-01 2018-03-02 黄世圆 The preparation method of honeysuckle disinfectant
CN110257184A (en) * 2019-06-27 2019-09-20 安徽省华凯轻工科技有限公司 A kind of food-grade alkali lotion

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