CN111000890A - Gel dressing for preventing and inhibiting HPV virus infection and preparation method thereof - Google Patents

Gel dressing for preventing and inhibiting HPV virus infection and preparation method thereof Download PDF

Info

Publication number
CN111000890A
CN111000890A CN201911398131.9A CN201911398131A CN111000890A CN 111000890 A CN111000890 A CN 111000890A CN 201911398131 A CN201911398131 A CN 201911398131A CN 111000890 A CN111000890 A CN 111000890A
Authority
CN
China
Prior art keywords
parts
extract
hydrogel
preparing
powder
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201911398131.9A
Other languages
Chinese (zh)
Inventor
杨国军
蒋清昀
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chongqing Gongmiao Biotechnology Co ltd
Original Assignee
Chongqing Gongmiao Biotechnology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chongqing Gongmiao Biotechnology Co ltd filed Critical Chongqing Gongmiao Biotechnology Co ltd
Priority to CN201911398131.9A priority Critical patent/CN111000890A/en
Publication of CN111000890A publication Critical patent/CN111000890A/en
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/489—Sophora, e.g. necklacepod or mamani
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
    • A61K31/35—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom
    • A61K31/352—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. methantheline 
    • A61K31/353—3,4-Dihydrobenzopyrans, e.g. chroman, catechin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • A61K31/731—Carrageenans
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/11—Pteridophyta or Filicophyta (ferns)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
    • A61K36/234—Cnidium (snowparsley)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/08—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing oxygen, e.g. ethers, acetals, ketones, quinones, aldehydes, peroxides
    • A61K47/10—Alcohols; Phenols; Salts thereof, e.g. glycerol; Polyethylene glycols [PEG]; Poloxamers; PEG/POE alkyl ethers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/08—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing oxygen, e.g. ethers, acetals, ketones, quinones, aldehydes, peroxides
    • A61K47/14—Esters of carboxylic acids, e.g. fatty acid monoglycerides, medium-chain triglycerides, parabens or PEG fatty acid esters
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/16—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing nitrogen, e.g. nitro-, nitroso-, azo-compounds, nitriles, cyanates
    • A61K47/18—Amines; Amides; Ureas; Quaternary ammonium compounds; Amino acids; Oligopeptides having up to five amino acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/26—Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
    • A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
    • A61K47/38—Cellulose; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
    • A61K47/42—Proteins; Polypeptides; Degradation products thereof; Derivatives thereof, e.g. albumin, gelatin or zein
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0034—Urogenital system, e.g. vagina, uterus, cervix, penis, scrotum, urethra, bladder; Personal lubricants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/06—Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/20—Antivirals for DNA viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Epidemiology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Medical Informatics (AREA)
  • Botany (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Inorganic Chemistry (AREA)
  • Reproductive Health (AREA)
  • Virology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Endocrinology (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Biochemistry (AREA)
  • Gynecology & Obstetrics (AREA)
  • Urology & Nephrology (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Medicinal Preparation (AREA)

Abstract

The invention discloses a gel dressing for preventing and inhibiting HPV virus infection, which consists of hydrogel and traditional Chinese medicine extracts uniformly dispersed in the hydrogel, wherein the traditional Chinese medicine extracts comprise the following components: the gel comprises a sophora flavescens extract, a scouring rush extract, a brucea javanica extract, a fructus cnidii extract, a celosin, carrageenan, a natural balance factor and borneol, wherein the hydrogel comprises sodium hydroxymethyl cellulose, ethylparaben, propylene glycol, mannitol, antibacterial peptide, glycerol, aqueous lubricating powder, diethanolamine and distilled water. By adopting the technical scheme, the anthocyanin is added, so that the effects of moistening skin, protecting heart, conditioning premenstrual syndrome and the like are achieved; the carrageenan is added, so that the immunity of a human body can be effectively improved, the genital tract is moistened, the mucous membrane is protected, and the symptoms of vaginal dryness and the like are effectively relieved.

Description

Gel dressing for preventing and inhibiting HPV virus infection and preparation method thereof
Technical Field
The invention relates to the technical field of female sanitary nursing products, in particular to a gel dressing for preventing and inhibiting HPV (human papillomavirus) virus infection and a preparation method thereof.
Background
Human Papillomavirus (HPV) infection is one of the most common gynecological infections. It has been reported that the onset of cervical malignancy may be associated with HPV infection, which induces cervical epithelial sarcoma and malignancy mainly by invasion of cervical epithelial cells. HPV viruses can be classified into low-risk types and high-risk types according to the severity of lesions caused by the HPV viruses. Wherein, the treatment with interferon is the most common, while the traditional Chinese medicine treatment can relatively reduce adverse reaction, avoid damaging normal organs and reduce the psychological burden of patients, thereby having certain advantages.
At present, the medicament for treating gynecological diseases is mainly administered through vagina, and the dosage forms of the common vagina administration preparations mainly comprise suppository, effervescent tablets, lotion, capsules and the like, but the dosage forms have some defects more or less and influence the exertion of the medicament. For example, the suppository has poor drug release, small contact surface with the vagina grinding, difficult infiltration of grinding folds, easy deformation at higher temperature and poor stability; for example, in the case of capsules, the drug is not easily released, and the coated surface is poor due to the disintegration of the capsules.
Disclosure of Invention
The invention provides a gel dressing for preventing and inhibiting HPV virus infection and a preparation method thereof.
In order to solve the technical problems, the invention adopts the technical scheme that the gel dressing for preventing and inhibiting HPV virus infection consists of hydrogel and traditional Chinese medicine extracts uniformly dispersed in the hydrogel, wherein the traditional Chinese medicine extracts comprise the following components in parts by weight: 15-25 parts of a sophora flavescens extract, 6-10 parts of a scouring rush extract, 4-7 parts of a brucea javanica extract, 12-15 parts of a fructus cnidii extract, 20-26 parts of a celosin, 6-10 parts of carrageenan, 3-5 parts of a natural balance factor and 4-6 parts of borneol, wherein the hydrogel comprises sodium hydroxymethyl cellulose, ethylparaben, propylene glycol, mannitol, antimicrobial peptide, glycerol, aqueous lubricating powder, diethanolamine and distilled water.
Furthermore, the hydrogel comprises the following components in parts by weight: 7-10 parts of sodium carboxymethylcellulose, 2-4 parts of ethylparaben, 12-16 parts of propylene glycol, 5-8 parts of mannitol, 5-8 parts of antibacterial peptide, 12-16 parts of glycerol, 9-15 parts of water-based lubricating powder, 3-6 parts of diethanolamine and 20-30 parts of distilled water.
The preparation method of the gel dressing for preventing and inhibiting HPV virus infection comprises the following steps,
(1) preparing a sophora flavescens extract: selecting radix sophorae flavescentis, cleaning with clear water, fishing out, drying, crushing, sieving with a 20-60-mesh sieve to obtain radix sophorae flavescentis powder, soaking with 2-4 times of 60-80% ethanol in volume for 20-30 h, and percolating at the flow rate of 5ml/min to obtain the radix sophorae flavescentis extract.
(2) Preparing a scouring rush extract: selecting scouring rush, removing impurities, drying in a dryer, crushing, sieving with a 120-200 mesh sieve to obtain scouring rush powder, putting the scouring rush powder in an ultrasonic extraction device, wherein the extracting solution is 60-80% ethanol, the extraction temperature is 35-45 ℃, and the material-liquid ratio is 1: 25-1: 15, so as to obtain the scouring rush extract.
(3) Preparing the brucea javanica extract: selecting brucea javanica seeds, removing impurities, drying in a dryer, crushing, sieving with a 120-200-mesh sieve to obtain brucea javanica powder, putting the brucea javanica powder in an ultrasonic extraction device to extract brucea javanica oil for 2-4 times, wherein the material-liquid ratio is 31-71, the extraction time is 15-25 hours, and the extraction temperature is 50-70 ℃ to obtain the brucea javanica extract.
(4) Preparing a common cnidium fruit extract: selecting fructus cnidii, removing impurities, drying in the sun, crushing, sieving with a sieve of 80-100 meshes to obtain fructus cnidii powder, soaking in a 200-time volume of 1% NaOH solution at 22-25 ℃ for 50-70 min, carrying out ultrasonic extraction for 8-12 min, adding 10% HCL into an extracting solution, acidifying, centrifuging, taking a precipitate, adding 5-10 times methanol into the precipitate for dissolving to obtain a dissolving solution, and filtering the dissolving solution by using a 0.45-micrometer microporous membrane to obtain the fructus cnidii extract.
(5) Preparing a hydrogel: in a preparation container, sequentially adding propylene glycol and antibacterial peptide according to parts by weight, stirring until compatibilization is transparent, adding mannitol, glycerol, sodium carboxymethylcellulose and ethylparaben, stirring uniformly, standing for 15-20 min, sequentially adding glycol amine, borneol, water-based lubricating powder and distilled water, and stirring uniformly to obtain the hydrogel.
(6) Preparing a traditional Chinese medicine extract mixture: and (3) uniformly mixing the traditional Chinese medicine extracts obtained in the steps (1) - (4) for later use.
(7) Preparing a gel dressing: adding the traditional Chinese medicine extract mixture into the hydrogel according to the parts by weight, uniformly stirring, then adding the anthocyanin, the carrageenan and the natural balance factor, mixing and stirring, and uniformly stirring to obtain the gel dressing.
Furthermore, in the step (7), after the gel dressing is uniformly stirred, the gel dressing is irradiated and disinfected by adopting an ultraviolet irradiation disinfection mode, wherein the irradiation time is 30-60 min.
By adopting the technical scheme, the anthocyanin is added, the anti-free radical oxidation capacity of the anthocyanin is 50 times that of vitamin E and 20 times that of vitamin C, and the anthocyanin can be completely and rapidly absorbed and has the effects of moistening skin, protecting heart, conditioning premenstrual syndrome and the like; the carrageenan is added, so that the immunity of a human body can be effectively improved, the genital tract can be moistened, the mucous membrane can be protected, and the symptoms of vaginal dryness, keratosis and the like can be effectively relieved; because the natural balance factors are added, the effect of activating balance is achieved; the scouring rush is added, and silicate and tannin contained in the scouring rush have an astringent effect and have the effects of diminishing inflammation and stopping bleeding on a contact part; adding brucea javanica extract: oleum fructus Bruceae has effects of clearing away heat and toxic materials.
Detailed Description
The following further describes the embodiments of the present invention. It should be noted that the description of the embodiments is provided to help understanding of the present invention, but the present invention is not limited thereto. In addition, the technical features involved in the embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
Fructus cnidii is a plant, also called wild carrot seed, which is picked in summer and autumn, and has mild temperament and bitter taste. The fructus Cnidii extract is extracted from fruit of cnidium monnieri (L.) DC of Umbelliferae, and contains pinene, isovalerian borneol ester, parsley phenol methyl ether, dihydrocarveol, bergapten, osthole, and isoanisic apigenin. The extract of the fructus cnidii mainly contains osthole, and the external application of the traditional Chinese medicine can treat trichomonas vaginitis, but the single fructus cnidii has poor efficacy and needs to be synthesized with other components into a compound preparation for treatment. The extraction of the cnidium fruit generally adopts a water decoction method, an ethanol ultrasonic extraction method and the like, and different extraction methods have different extraction results on the osthole. The table below shows the osthole extraction content for different extraction methods.
TABLE-Effect of different extraction methods on the extraction of osthole from cnidium fruit
Extraction method Cnidium lactone content (mg \ g-l)
Water decoction method 3.784
Ultrasonic extraction method of ethanol 19.581
Alkaline impregnation process 20.763
Ultrasonic extraction method by alkali dissolution and acid precipitation 22.497
Therefore, the alkali-dissolution acid-precipitation ultrasonic extraction method is the best extraction method of osthole.
The first embodiment is as follows:
the gel dressing for preventing and inhibiting HPV virus infection consists of hydrogel and traditional Chinese medicine extracts uniformly dispersed in the hydrogel, wherein the traditional Chinese medicine extracts comprise the following components in parts by weight: 15 parts of coconut extract, 15 parts of sophora flavescens extract, 6 parts of scouring rush extract, 4 parts of brucea javanica extract, 12 parts of fructus cnidii extract, 20 parts of anthocyanin, 6 parts of carrageenan, 3 parts of natural balance factor and 4 parts of borneol, wherein the hydrogel comprises sodium hydroxymethyl cellulose, ethylparaben, propylene glycol, mannitol, antibacterial peptide, glycerol, aqueous lubricating powder, diethanolamine and distilled water.
The hydrogel comprises the following components in parts by weight: 7 parts of sodium carboxymethylcellulose, 2 parts of ethylparaben, 12 parts of propylene glycol, 5 parts of mannitol, 5 parts of antibacterial peptide, 12 parts of glycerol, 9 parts of aqueous lubricating powder, 3 parts of diethanolamine and 20 parts of distilled water.
The preparation method of the gel dressing for preventing and inhibiting HPV virus infection comprises the following steps,
(1) preparing a sophora flavescens extract: selecting radix Sophorae Flavescentis, cleaning with clear water, taking out, oven drying, pulverizing, sieving with 20 mesh sieve to obtain radix Sophorae Flavescentis powder, soaking in 2 times volume of 60% ethanol for 20 hr, and percolating at flow rate of 5ml/min to obtain radix Sophorae Flavescentis extract.
(2) Preparing a scouring rush extract: selecting scouring rush, removing impurities, drying in a dryer, crushing, sieving with a 120-mesh sieve to obtain scouring rush powder, putting the scouring rush powder in an ultrasonic extraction device, wherein the extracting solution is 60% ethanol, the extraction temperature is 35 ℃, and the material-liquid ratio is 1:25 to obtain the scouring rush extract.
(3) Preparing the brucea javanica extract: selecting brucea javanica seeds, removing impurities, drying in a dryer, crushing, sieving with a 120-mesh sieve to obtain brucea javanica powder, putting the brucea javanica powder in an ultrasonic extraction device to extract brucea javanica oil for 2 times, wherein the material-liquid ratio is 31, the extraction time is 15 hours, and the extraction temperature is 50 ℃ to obtain the brucea javanica extract.
(4) Preparing a common cnidium fruit extract: selecting fructus Cnidii, removing impurities, sun drying, pulverizing, sieving with 80 mesh sieve to obtain fructus Cnidii powder, soaking with 200 times volume of 1% NaOH solution at 22 deg.C for 50min, ultrasonic extracting for 8min, adding 10% HCl into the extractive solution, acidifying, centrifuging, collecting precipitate, adding 5 times methanol into the precipitate for dissolving to obtain solution, and filtering with 0.45 μm microporous membrane to obtain fructus Cnidii extract.
(5) Preparing a hydrogel: in a preparation container, sequentially adding propylene glycol and antibacterial peptide according to parts by weight, stirring until compatibilization is transparent, adding mannitol, glycerol, sodium carboxymethylcellulose and ethylparaben, stirring uniformly, standing for 15min, sequentially adding glycol amine, borneol, water-based lubricating powder and distilled water, and stirring uniformly to obtain the hydrogel.
(6) Preparing a traditional Chinese medicine extract mixture: and (3) uniformly mixing the traditional Chinese medicine extracts obtained in the steps (1) - (4) for later use.
(7) Preparing a gel dressing: adding the traditional Chinese medicine extract mixture into the hydrogel according to the weight part, uniformly stirring, then adding the anthocyanin, the carrageenan and the natural balance factor, mixing and stirring, uniformly stirring, and then irradiating and disinfecting the gel dressing by adopting an ultraviolet irradiation disinfection mode for 30-60 min to obtain the gel dressing.
Example two:
the gel dressing for preventing and inhibiting HPV virus infection consists of hydrogel and traditional Chinese medicine extracts uniformly dispersed in the hydrogel, wherein the traditional Chinese medicine extracts comprise the following components in parts by weight: 16 parts of coconut extract, 20 parts of sophora flavescens extract, 8 parts of scouring rush extract, 5 parts of brucea javanica extract, 13 parts of fructus cnidii extract, 23 parts of anthocyanin, 8 parts of carrageenan, 4 parts of natural balance factor and 5 parts of borneol, wherein the hydrogel comprises sodium hydroxymethyl cellulose, ethylparaben, propylene glycol, mannitol, antibacterial peptide, glycerol, aqueous lubricating powder, diethanolamine and distilled water.
The hydrogel comprises the following components in parts by weight: 8 parts of sodium carboxymethylcellulose, 3 parts of ethylparaben, 14 parts of propylene glycol, 6 parts of mannitol, 6 parts of antibacterial peptide, 14 parts of glycerol, 12 parts of aqueous lubricating powder, 5 parts of diethanolamine and 25 parts of distilled water.
The preparation method of the gel dressing for preventing and inhibiting HPV virus infection comprises the following steps,
(1) preparing a sophora flavescens extract: selecting radix Sophorae Flavescentis, cleaning with clear water, taking out, oven drying, pulverizing, sieving with 40 mesh sieve to obtain radix Sophorae Flavescentis powder, soaking in 3 times volume of 70% ethanol for 25 hr, and percolating at flow rate of 5ml/min to obtain radix Sophorae Flavescentis extract.
(2) Preparing a scouring rush extract: selecting scouring rush, removing impurities, drying in a dryer, crushing, sieving with a 160-mesh sieve to obtain scouring rush powder, putting the scouring rush powder in an ultrasonic extraction device, wherein the extracting solution is 70% ethanol, the extraction temperature is 40 ℃, and the material-liquid ratio is 1:20 to obtain the scouring rush extract.
(3) Preparing the brucea javanica extract: selecting brucea javanica seeds, removing impurities, drying in a dryer, crushing, sieving with a 160-mesh sieve to obtain brucea javanica powder, putting the brucea javanica powder in an ultrasonic extraction device to extract brucea javanica oil for 3 times, wherein the material-liquid ratio is 51, the extraction time is 20 hours, and the extraction temperature is 60 ℃ to obtain the brucea javanica extract.
(4) Preparing a common cnidium fruit extract: selecting fructus Cnidii, removing impurities, sun drying, pulverizing, sieving with 90 mesh sieve to obtain fructus Cnidii powder, soaking with 200 times volume of 1% NaOH solution at 23 deg.C for 60min, ultrasonic extracting for 10min, adding 10% HCl into the extractive solution, acidifying, centrifuging, collecting precipitate, adding 8 times volume of methanol into the precipitate for dissolving to obtain solution, and filtering with 0.45 μm microporous membrane to obtain fructus Cnidii extract.
(5) Preparing a hydrogel: in a preparation container, sequentially adding propylene glycol and antibacterial peptide according to parts by weight, stirring until compatibilization is transparent, adding mannitol, glycerol, sodium carboxymethylcellulose and ethylparaben, stirring uniformly, standing for 18min, sequentially adding glycol amine, borneol, water-based lubricating powder and distilled water, and stirring uniformly to obtain the hydrogel.
(6) Preparing a traditional Chinese medicine extract mixture: and (3) uniformly mixing the traditional Chinese medicine extracts obtained in the steps (1) - (4) for later use.
(7) Preparing a gel dressing: adding the traditional Chinese medicine extract mixture into the hydrogel according to the weight part, uniformly stirring, adding the anthocyanin, the carrageenan and the natural balance factor, mixing and stirring, uniformly stirring, and irradiating and disinfecting the gel dressing by adopting an ultraviolet irradiation disinfection mode for 45min to obtain the gel dressing.
Example three:
the gel dressing for preventing and inhibiting HPV virus infection consists of hydrogel and traditional Chinese medicine extracts uniformly dispersed in the hydrogel, wherein the traditional Chinese medicine extracts comprise the following components in parts by weight: 18 parts of coconut extract, 25 parts of sophora flavescens extract, 10 parts of scouring rush extract, 7 parts of brucea javanica extract, 15 parts of fructus cnidii extract, 26 parts of anthocyanin, 10 parts of carrageenan, 5 parts of natural balance factor and 6 parts of borneol, wherein the hydrogel comprises sodium hydroxymethyl cellulose, ethylparaben, propylene glycol, mannitol, antibacterial peptide, glycerol, aqueous lubricating powder, diethanolamine and distilled water.
The hydrogel comprises the following components in parts by weight: 10 parts of sodium carboxymethylcellulose, 4 parts of ethylparaben, 16 parts of propylene glycol, 8 parts of mannitol, 8 parts of antibacterial peptide, 16 parts of glycerol, 15 parts of aqueous lubricating powder, 6 parts of diethanolamine and 30 parts of distilled water.
The preparation method of the gel dressing for preventing and inhibiting HPV virus infection comprises the following steps,
(1) preparing a sophora flavescens extract: selecting radix Sophorae Flavescentis, cleaning with clear water, taking out, oven drying, pulverizing, sieving with 60 mesh sieve to obtain radix Sophorae Flavescentis powder, soaking in 4 times volume of 80% ethanol for 30 hr, and percolating at flow rate of 5ml/min to obtain radix Sophorae Flavescentis extract.
(2) Preparing a scouring rush extract: selecting scouring rush, removing impurities, drying in a dryer, crushing, sieving with a 200-mesh sieve to obtain scouring rush powder, putting the scouring rush powder in an ultrasonic extraction device, wherein the extracting solution is 80% ethanol, the extraction temperature is 45 ℃, and the material-liquid ratio is 1:15 to obtain the scouring rush extract.
(3) Preparing the brucea javanica extract: selecting brucea javanica seeds, removing impurities, drying in a dryer, crushing, sieving with a 200-mesh sieve to obtain brucea javanica powder, putting the brucea javanica powder in an ultrasonic extraction device to extract brucea javanica oil for 4 times, wherein the material-liquid ratio is 71, the extraction time is 25 hours, and the extraction temperature is 70 ℃, so as to obtain the brucea javanica extract.
(4) Preparing a common cnidium fruit extract: selecting fructus Cnidii, removing impurities, sun drying, pulverizing, sieving with 100 mesh sieve to obtain fructus Cnidii powder, soaking with 200 times volume of 1% NaOH solution at 25 deg.C for 70min, ultrasonic extracting for 12min, adding 10% HCl into the extractive solution, acidifying, centrifuging, collecting precipitate, adding 10 times volume of methanol into the precipitate, dissolving to obtain solution, and filtering with 0.45 μm microporous membrane to obtain fructus Cnidii extract.
(5) Preparing a hydrogel: in a preparation container, sequentially adding propylene glycol and antibacterial peptide according to parts by weight, stirring until compatibilization is transparent, adding mannitol, glycerol, sodium carboxymethylcellulose and ethylparaben, stirring uniformly, standing for 20min, sequentially adding glycol amine, borneol, water-based lubricating powder and distilled water, and stirring uniformly to obtain the hydrogel.
(6) Preparing a traditional Chinese medicine extract mixture: and (3) uniformly mixing the traditional Chinese medicine extracts obtained in the steps (1) - (4) for later use.
(7) Preparing a gel dressing: adding the traditional Chinese medicine extract mixture into the hydrogel according to the weight part, uniformly stirring, then adding the anthocyanin, the carrageenan and the natural balance factor, mixing and stirring, uniformly stirring, and then irradiating and disinfecting the gel dressing by adopting an ultraviolet irradiation disinfection mode for 30-60 min to obtain the gel dressing.
150 subjects (all the cervical parts have erosive surfaces, namely, the possibility of HPV infection exists) are invited to use the gel dressings for preventing and inhibiting HPV virus infection prepared in the first example, the second example and the third example respectively, and the feedback results of the subjects after 1 month are obtained as follows:
turning negative: cervical smoothness and disappearance of erosion surface
TABLE II comparison of negative turning for the first, second and third examples
Group of Number of cases in group Number of cases with negative change Probability of turning negative
Example one 50 42 84％
Example two 50 48 96％
EXAMPLE III 50 45 90％
In summary, the second embodiment is the best embodiment.
The invention is not limited to the described embodiments. It will be apparent to those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, and the scope of protection is still within the scope of the invention.

Claims (4)

1. A gel dressing for preventing and inhibiting HPV viral infection, characterized in that: the hydrogel comprises hydrogel and a traditional Chinese medicine extract uniformly dispersed in the hydrogel, wherein the traditional Chinese medicine extract comprises the following components in parts by weight: 15-25 parts of a sophora flavescens extract, 6-10 parts of a scouring rush extract, 4-7 parts of a brucea javanica extract, 12-15 parts of a fructus cnidii extract, 20-26 parts of a celosin, 6-10 parts of carrageenan, 3-5 parts of a natural balance factor and 4-6 parts of borneol, wherein the hydrogel comprises sodium hydroxymethyl cellulose, ethylparaben, propylene glycol, mannitol, antimicrobial peptide, glycerol, aqueous lubricating powder, diethanolamine and distilled water.
2. The gel dressing for preventing and inhibiting HPV viral infection according to claim 1, wherein: the hydrogel comprises the following components in parts by weight: 7-10 parts of sodium carboxymethylcellulose, 2-4 parts of ethylparaben, 12-16 parts of propylene glycol, 5-8 parts of mannitol, 5-8 parts of antibacterial peptide, 12-16 parts of glycerol, 9-15 parts of water-based lubricating powder, 3-6 parts of diethanolamine and 20-30 parts of distilled water.
3. The method for preparing a gel dressing for preventing and inhibiting HPV viral infection according to claim 1, wherein the method comprises the following steps: comprises the following steps of (a) carrying out,
(1) preparing a sophora flavescens extract: selecting radix sophorae flavescentis, cleaning with clear water, fishing out, drying, crushing, sieving with a 20-60-mesh sieve to obtain radix sophorae flavescentis powder, soaking with 2-4 times of 60-80% ethanol in volume for 20-30 h, and percolating at the flow rate of 5ml/min to obtain a radix sophorae flavescentis extract;
(2) preparing a scouring rush extract: selecting scouring rush, removing impurities, drying in a dryer, crushing, sieving with a 120-200 mesh sieve to obtain scouring rush powder, putting the scouring rush powder in an ultrasonic extraction device, wherein the extracting solution is 60-80% ethanol, the extraction temperature is 35-45 ℃, and the material-liquid ratio is 1: 25-1: 15 to obtain the scouring rush extract;
(3) preparing the brucea javanica extract: selecting brucea javanica seeds, removing impurities, drying in a dryer, crushing, sieving with a 120-200-mesh sieve to obtain brucea javanica powder, putting the brucea javanica powder in an ultrasonic extraction device to extract brucea javanica oil for 2-4 times, wherein the material-liquid ratio is 31-71, the extraction time is 15-25 hours, and the extraction temperature is 50-70 ℃ to obtain a brucea javanica extract;
(4) preparing a common cnidium fruit extract: selecting fructus cnidii, removing impurities, drying in the sun, crushing, sieving with a sieve of 80-100 meshes to obtain fructus cnidii powder, soaking in a 200-time volume of 1% NaOH solution at 22-25 ℃ for 50-70 min, carrying out ultrasonic extraction for 8-12 min, adding 10% HCL into an extracting solution, acidifying, centrifuging, taking a precipitate, adding 5-10 times methanol into the precipitate for dissolving to obtain a dissolving solution, and filtering the dissolving solution by using a 0.45-micrometer microporous membrane to obtain a fructus cnidii extract;
(5) preparing a hydrogel: sequentially adding propylene glycol and antibacterial peptide in parts by weight in a preparation container, stirring until the volume is increased and transparent, adding mannitol, glycerol, sodium carboxymethylcellulose and ethylparaben, stirring uniformly, standing for 15-20 min, sequentially adding glycol amine, borneol, water-based lubricating powder and distilled water, and stirring uniformly to obtain hydrogel;
(6) preparing a traditional Chinese medicine extract mixture: uniformly mixing the traditional Chinese medicine extracts obtained in the steps (1) - (4) for later use;
(7) preparing a gel dressing: adding the traditional Chinese medicine extract mixture into the hydrogel according to the parts by weight, uniformly stirring, then adding the anthocyanin, the carrageenan and the natural balance factor, mixing and stirring, and uniformly stirring to obtain the gel dressing.
4. The method for preparing a gel dressing for preventing and inhibiting HPV viral infection according to claim 1, wherein the method comprises the following steps: and (7) after uniformly stirring, irradiating and disinfecting the gel dressing by adopting an ultraviolet irradiation disinfection mode, wherein the irradiation time is 30-60 min.
CN201911398131.9A 2019-12-30 2019-12-30 Gel dressing for preventing and inhibiting HPV virus infection and preparation method thereof Pending CN111000890A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201911398131.9A CN111000890A (en) 2019-12-30 2019-12-30 Gel dressing for preventing and inhibiting HPV virus infection and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201911398131.9A CN111000890A (en) 2019-12-30 2019-12-30 Gel dressing for preventing and inhibiting HPV virus infection and preparation method thereof

Publications (1)

Publication Number Publication Date
CN111000890A true CN111000890A (en) 2020-04-14

Family

ID=70119346

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201911398131.9A Pending CN111000890A (en) 2019-12-30 2019-12-30 Gel dressing for preventing and inhibiting HPV virus infection and preparation method thereof

Country Status (1)

Country Link
CN (1) CN111000890A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112220739A (en) * 2020-10-15 2021-01-15 国润生物科技（深圳）有限公司 HPV virus inactivation dressing and preparation method thereof
CN113440559A (en) * 2021-06-04 2021-09-28 高丽莉 anti-HPV gel and preparation method thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1562170A (en) * 2004-03-22 2005-01-12 沈阳药科大学 'Mikang' gels of Chinese traditional medicine for curing inflammation of cervical erosion etc. and preparation method
CN1915319A (en) * 2006-08-21 2007-02-21 北京润德康医药技术有限公司 Composition of Chinese traditional medicine in use for treating female inflammation
CN106729227A (en) * 2017-01-18 2017-05-31 张勇 It is a kind of to treat pure Chinese medicine gel of HPV infection cervical carcinoma and preparation method thereof
CN107213177A (en) * 2017-06-15 2017-09-29 陕西海天制药有限公司 A kind of preparation method for the Chinese medicine composition and its gel for treating cervical erosion

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1562170A (en) * 2004-03-22 2005-01-12 沈阳药科大学 'Mikang' gels of Chinese traditional medicine for curing inflammation of cervical erosion etc. and preparation method
CN1915319A (en) * 2006-08-21 2007-02-21 北京润德康医药技术有限公司 Composition of Chinese traditional medicine in use for treating female inflammation
CN106729227A (en) * 2017-01-18 2017-05-31 张勇 It is a kind of to treat pure Chinese medicine gel of HPV infection cervical carcinoma and preparation method thereof
CN107213177A (en) * 2017-06-15 2017-09-29 陕西海天制药有限公司 A kind of preparation method for the Chinese medicine composition and its gel for treating cervical erosion

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
任茹霞，等: "阴离子多聚物类杀微生物剂的研究进展", 《中国药房》 *
巫君玉，等: "《现代难治病中医诊疗学》", 31 July 1993, 中医古籍出版社 *
郁慧: ""康泰乐——除疣擦剂"治疗尖锐湿疣180例", 《皮肤病与性病》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112220739A (en) * 2020-10-15 2021-01-15 国润生物科技（深圳）有限公司 HPV virus inactivation dressing and preparation method thereof
CN112220739B (en) * 2020-10-15 2023-09-01 国润生物科技（深圳）有限公司 HPV virus inactivation dressing and preparation method thereof
CN113440559A (en) * 2021-06-04 2021-09-28 高丽莉 anti-HPV gel and preparation method thereof

Similar Documents

Publication Publication Date Title
CN103040924A (en) Radix millettiae speciosae extract and application thereof in preparing drugs for treating osteoporosis
CN103110868A (en) Skin care solution and preparation method thereof
CN111000890A (en) Gel dressing for preventing and inhibiting HPV virus infection and preparation method thereof
CN101152324A (en) Method for producing Yujin composition and medicament containing the same
CN103263525B (en) Gynecological cleaning fluid and preparation method thereof
EP3821902B1 (en) Traditional chinese medicine composition, traditional chinese medicine compound preparation and preparation method and use thereof
CN105535867A (en) Antibacterial gel special for gynecology and preparation method thereof
KR101418084B1 (en) method for preparing microgel containing capsuled atopic dermatitis treatment composition
CN107308333A (en) It is a kind of to promote Chinese medicine composition of wound healing and preparation method thereof
CN106214740A (en) A kind of Chinese medicine composition and its production and use
CN105853980A (en) Medicine composition for treating wound infection after vein blood sampling and preparing method thereof
CN1043406C (en) Method for preparation of compound Qingdaisan powder
CN1319582C (en) Chinese medicine solution agent for treating damp-heat disease and its preparing method
CN102552796B (en) Chinese medicinal composition for treating chronic gastric ulcer
CN109847015B (en) Traditional Chinese medicine lotion and preparation method and application thereof
CN112022947A (en) Bacteriostatic agent based on traditional Chinese herbal medicines and small molecular compounds, and preparation method and application thereof
CN105853614A (en) Gynaecological capsule
CN105148080A (en) Mecodium badium and rutin compound nursing medicament for treating skin injury
CN104173576A (en) Tobacco-containing traditional Chinese medicine temperature-sensitive gel agent for treating hemorrhoids
CN106236888A (en) A kind of treat the pharmaceutical composition of wound circumference congestion after blood drawing
CN109999111A (en) A kind of pharmaceutical composition and preparation method thereof for treating acne
CN115645508B (en) External traditional Chinese medicine composition and external preparation for cervical diseases and preparation method thereof
CN104257871B (en) Scorching pharmaceutical composition of the acute lymphatic meshwork of type and preparation method thereof is accumulate for treating damp and hot poison
CN109172732A (en) A kind of Chinese medicine composition and the preparation method and application thereof of anti-HPV
RU2810482C1 (en) Medicinal composition of tcm and its use in prevention and treatment of viral pneumonia

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20200414

RJ01 Rejection of invention patent application after publication