CN110946038A - Formula and cultivation method of efficient industrial culture medium for sparassis crispa - Google Patents
Formula and cultivation method of efficient industrial culture medium for sparassis crispa Download PDFInfo
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- CN110946038A CN110946038A CN201911097010.0A CN201911097010A CN110946038A CN 110946038 A CN110946038 A CN 110946038A CN 201911097010 A CN201911097010 A CN 201911097010A CN 110946038 A CN110946038 A CN 110946038A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
Abstract
The invention discloses a high-efficiency industrial culture medium formula and a culture method for sparassis crispa, wherein the culture medium formula comprises the following components in percentage by dry weight: 75-90% of sawdust, 6-15% of barley, 3-5% of corncob and 3-7% of cotton seed; the cultivation method comprises the steps of culture material preparation, packaging, high temperature, high pressure sterilization, inoculation, cultivation, mushroom opening and collection. The method has the beneficial effects of shortening the production period and reducing the contamination probability of the bacteria in the fungus package, and is mainly used for high-efficiency production of the Sparassis crispa.
Description
Technical Field
The invention relates to the technical field of edible fungus culture, in particular to a formula and a culture method of a high-efficiency industrial culture medium for sparassis crispa.
Background
Sparassiscripa, Sparassiscripa crispa, Sparassis crispa, and nidus Vespae, etc., the Latin is named as Sparassiscriscrispa, is of Aphyllophorales, Sparassicaceae, Sparassis, fruiting bodies of Mesorhizoma are medium to large in shape, and in meat quality, a large number of branches are sent out from a thick handle, and countless zigzag petals are formed at the branch ends, so that the Sparassisca crispa is obtained.
The present culture method of sparassis crispa, potato powder, flour and peptone has the formula of containing 100 wt% of the above-mentioned components, and its result is long period, generally above 150 days, need to consume a lot of electric energy, and is easy to cause various bacteria infection, and its yield is below 180 g/bag, so that its production cost is high, so that it is not suitable for industrial mass production.
Disclosure of Invention
The invention aims to solve the technical problems of providing a formula of a high-efficiency industrial culture medium for Sparassis crispa and a culture method for Sparassis crispa, which are capable of shortening the production period and reducing the contamination probability of bacteria in bags, and solving the problems described in the background technology.
In order to solve the technical problems, the technical scheme of the invention is as follows:
the invention is realized in such a way that a formula of a high-efficiency industrial culture medium for sparassis crispa is as follows according to the dry weight percentage:
75-90% of wood chips, 6-15% of barley, 3-5% of corncobs and 3-7% of cotton seeds, wherein the weight percentage of each component is 100%, the culture medium is stirred by adding water, and the water content after stirring is controlled to be 58-66%.
Preferably, the wood chips are larch wood chips.
The invention also provides a high-efficiency industrial culture process of the sparassis crispa based on the formula, which comprises the following steps:
s1, preparing culture materials:
weighing a proper amount of sawdust, barley, corncobs and cotton seeds, mixing the sawdust, the barley, the corncobs and the cotton seeds, adding water, and uniformly stirring to obtain a culture material with the water content of 58-66%;
s2, packaging: filling the obtained culture material into a culture bag and packaging;
s3, high-temperature and high-pressure sterilization: the method is carried out high-temperature and high-pressure sterilization, the pressure is set to be 0.10-0.24 Mpa, and the temperature and the time can be any one of the following three modes:
①, the temperature is 105 ℃, and the time is 80-110 minutes;
②, the temperature is 110 ℃, and the time is 50-80 minutes;
③, the temperature is 116-125 ℃, and the time is 90-120 minutes;
s4, inoculation:
sterilizing at high temperature and high pressure, cooling to below 25 deg.C, and inoculating in sterile room;
s5, culturing:
placing the inoculated strain in a non-illumination environment for 5-9 days, then irradiating with low light for 8-12 hours every day within 10-20 days, irradiating with strong light for 8-12 hours every day within 20-35 days, controlling the temperature at 18-25 ℃ and the humidity at 60-80% in the culture process;
s6, mushroom opening:
when the strain is gradually twisted to form primordium and the lamella is differentiated, strong light is adopted for 15-30 days, 10-15 hours are taken every day, the temperature is controlled at 18-25 ℃, and the humidity is controlled at more than 90%;
and S7, collecting.
Compared with the prior art, the invention has the beneficial effects that: the present invention shortens the production cycle, reduces the production cost, has a great economic effect, reduces the probability of contamination with bacteria in the fungus bundles due to the short production cycle, improves the production efficiency by reducing contamination with bacteria between the fungus bundles, and reduces the amount of electric power used by shortening the production time, thereby achieving an energy saving effect.
Drawings
FIG. 1 is a schematic structural diagram of the cultivation method of the present invention.
Detailed Description
The following further describes embodiments of the present invention with reference to the drawings. It should be noted that the description of the embodiments is provided to help understanding of the present invention, but the present invention is not limited thereto. In addition, the technical features involved in the embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
Example one
The invention provides a technical scheme that: the formula of the sparassis crispa high-efficiency industrial culture medium comprises the following components in percentage by dry weight:
75-90% of wood chips, 6-15% of barley, 3-5% of corncobs and 3-7% of cotton seeds, wherein the weight percentage of each component is 100%, the culture medium is stirred by adding water, and the water content after stirring is controlled to be 58-66%.
Wherein the wood chips are larch wood chips.
Referring to fig. 1, the present invention further provides a high efficiency industrial culture process of sparassis crispa based on the above formula, comprising the following steps:
s1, preparing culture materials:
weighing a proper amount of sawdust, barley, corncobs and cotton seeds according to the formula of claim 1, mixing the sawdust, the barley, the corncobs and the cotton seeds, adding water, and uniformly stirring to obtain a culture material with the water content of 58-66%;
s2, packaging:
filling the obtained culture material into a culture bag and packaging;
s3, high-temperature and high-pressure sterilization:
the method is carried out high-temperature and high-pressure sterilization, the pressure is set to be 0.10-0.24 Mpa, and the temperature and the time can be any one of the following three modes:
①, the temperature is 105 ℃, and the time is 80-110 minutes and 50 minutes;
②, the temperature is 110 ℃, and the time is 50-80 minutes;
③, the temperature is 116-125 ℃, and the time is 90-120 minutes;
s4, inoculation:
sterilizing at high temperature and high pressure, cooling to below 25 deg.C, and inoculating in sterile room;
s5, culturing:
placing the inoculated strain in a non-illumination environment for 5-9 days, then irradiating with low light for 8-12 hours every day within 10-20 days, irradiating with strong light for 8-12 hours every day within 20-35 days, controlling the temperature at 18-25 ℃ and the humidity at 60-80% in the culture process;
s6, mushroom opening:
when the strain is gradually twisted to form primordium and the lamella is differentiated, strong light is adopted for 15-30 days, 10-15 hours are taken every day, the temperature is controlled at 18-25 ℃, and the humidity is controlled at more than 90%;
and S7, collecting.
Example two
The formula of the sparassis crispa high-efficiency industrial culture medium comprises the following components in percentage by dry weight:
75% of wood chips, 15% of barley, 3% of corncobs and 7% of cotton seeds, wherein the weight percentage of the components is 100%, the culture medium is stirred by adding water, the water content after stirring is controlled to be 58-66%, and the wood chips are larch wood chips.
Referring to fig. 1, the method for cultivating the sparassis crispa in an efficient factory based on the formula comprises the following steps:
s1, preparing culture materials:
weighing a proper amount of sawdust, barley, corncobs and cotton seeds according to the formula of claim 1, mixing the sawdust, the barley, the corncobs and the cotton seeds, adding water, and uniformly stirring to obtain a culture material with the water content of 58-66%;
s2, packaging:
filling the obtained culture material into a culture bag and packaging;
s3, high-temperature and high-pressure sterilization:
sterilizing the mixture at high temperature and high pressure, wherein the pressure is set to be 0.10-0.24 Mpa, the temperature is 105 ℃, and the time is 90-120 minutes;
s4, inoculation:
sterilizing at high temperature and high pressure, cooling to below 25 deg.C, and inoculating in sterile room;
s5, culturing:
placing the inoculated strain in a non-illumination environment for 5-9 days, then irradiating with low light for 8-12 hours every day within 10-20 days, irradiating with strong light for 8-12 hours every day within 20-35 days, controlling the temperature at 18-25 ℃ and the humidity at 60-80% in the culture process;
s6, mushroom opening:
when the strain is gradually twisted to form primordium and the lamella is differentiated, strong light is adopted for 15-30 days, 10-15 hours are taken every day, the temperature is controlled at 18-25 ℃, and the humidity is controlled at more than 90%;
and S7, collecting.
EXAMPLE III
The formula of the sparassis crispa high-efficiency industrial culture medium comprises the following components in percentage by dry weight:
80% of wood chips, 10% of barley, 5% of corncobs and 5% of cotton seeds, wherein the weight percentage of the components is 100%, the culture medium is stirred by adding water, the water content after stirring is controlled to be 58-66%, and the wood chips are larch wood chips.
Referring to fig. 1, the method for cultivating the sparassis crispa in an efficient factory based on the formula comprises the following steps:
s1, preparing culture materials:
weighing a proper amount of sawdust, barley, corncobs and cotton seeds according to the formula of claim 1, mixing the sawdust, the barley, the corncobs and the cotton seeds, adding water, and uniformly stirring to obtain a culture material with the water content of 58-66%;
s2, packaging:
filling the obtained culture material into a culture bag and packaging;
s3, high-temperature and high-pressure sterilization:
sterilizing the mixture at high temperature and high pressure, wherein the pressure is set to be 0.10-0.24 Mpa, the temperature is 115-125 ℃, and the time is 90 minutes;
s4, inoculation:
sterilizing at high temperature and high pressure, cooling to below 25 deg.C, and inoculating in sterile room;
s5, culturing:
placing the inoculated strain in a non-illumination environment for 5-9 days, then irradiating with low light for 8-12 hours every day within 10-20 days, irradiating with strong light for 8-12 hours every day within 20-35 days, controlling the temperature at 18-25 ℃ and the humidity at 60-80% in the culture process;
s6, mushroom opening:
when the strain is gradually twisted to form primordium and the lamella is differentiated, strong light is adopted for 15-30 days, 10-15 hours are taken every day, the temperature is controlled at 18-25 ℃, and the humidity is controlled at more than 90%;
and S7, collecting.
The embodiments of the present invention have been described in detail with reference to the accompanying drawings, but the present invention is not limited to the described embodiments. It will be apparent to those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, and the scope of protection is still within the scope of the invention.
Claims (4)
1. A formula of a high-efficiency industrial culture medium for sparassis crispa is characterized in that: the formula of the culture medium comprises the following components in percentage by dry weight:
75-90% of wood chips, 6-15% of barley, 3-5% of corncobs and 3-7% of cotton seeds, wherein the weight percentage of each component is 100%, the culture medium is stirred by adding water, and the water content after stirring is controlled to be 58-66%.
2. The formula of the high-efficiency industrial culture medium for the sparassis crispa according to claim 1, which is characterized in that: the sawdust is larch sawdust.
3. A high-efficiency industrial culture method of sparassis crispa is characterized in that: the method comprises the following steps:
s1, preparing culture materials:
weighing a proper amount of sawdust, barley, corncobs and cotton seeds according to the formula of claim 1, mixing the sawdust, the barley, the corncobs and the cotton seeds, adding water, and uniformly stirring to obtain a culture material with the water content of 58-66%;
s2, packaging:
filling the obtained culture material into a culture bag and packaging;
s3, high-temperature and high-pressure sterilization:
sterilizing at high temperature and high pressure with pressure of 0.10-0.24 Mpa at the temperature and time
Any one of the following three modes:
①, the temperature is 105 ℃, and the time is 80-110 minutes;
②, the temperature is 110 ℃, and the time is 50-80 minutes;
③, the temperature is 116-125 ℃, and the time is 90-120 minutes;
s4, inoculation:
sterilizing at high temperature and high pressure, cooling to below 25 deg.C, and inoculating in sterile room;
s5, culturing:
placing the inoculated strain in a non-illumination environment for 5-9 days, then irradiating with low light for 8-12 hours every day within 10-20 days, irradiating with strong light for 8-12 hours every day within 20-35 days, controlling the temperature at 18-25 ℃ and the humidity at 60-80% in the culture process;
s6, mushroom opening:
when the strain is gradually twisted to form primordium and the lamella is differentiated, strong light is adopted for 15-30 days, 10-15 hours are taken every day, the temperature is controlled at 18-25 ℃, and the humidity is controlled at more than 90%;
and S7, collecting.
4. The method for industrially cultivating Sparassis crispa with high efficiency as claimed in claim 3, wherein the method comprises the following steps: the specification of the cultivation bag adopts a polypropylene plastic bag with the thickness of 17cm multiplied by 33cm multiplied by 0.005 cm.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111713335A (en) * | 2020-07-01 | 2020-09-29 | 福建省农业科学院食用菌研究所（福建省蘑菇菌种研究推广站） | Efficient sparassis crispa cultivation method |
| CN113615480A (en) * | 2021-08-11 | 2021-11-09 | 杜捷 | Sparassis crispa culture medium and preparation method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111713335A (en) * | 2020-07-01 | 2020-09-29 | 福建省农业科学院食用菌研究所（福建省蘑菇菌种研究推广站） | Efficient sparassis crispa cultivation method |
| CN111713335B (en) * | 2020-07-01 | 2022-04-12 | 福建省农业科学院食用菌研究所（福建省蘑菇菌种研究推广站） | Efficient sparassis crispa cultivation method |
| CN113615480A (en) * | 2021-08-11 | 2021-11-09 | 杜捷 | Sparassis crispa culture medium and preparation method thereof |
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Application publication date: 20200403 |