CN110893202A - Medicinal liquor for treating gout - Google Patents

Abstract

The invention provides medicinal liquor for treating gout, which has the effects of reducing uric acid, resisting inflammation, relieving swelling and pain and the like. A medicinal liquor for treating gout is prepared from the following raw materials in parts by weight: 15-20 parts of plutella xylostella, 1-2 parts of ephedra, 1-2 parts of cassia twig and 1-2 parts of angelica. The preparation method of the medicinal liquor comprises the following steps: cleaning the above materials, pulverizing, placing in a porcelain jar or glass jar, adding 90-130 parts of 40-70% (v/v) Chinese liquor, sealing, and standing for 34-50 days.

Description

Medicinal liquor for treating gout

Technical Field

The invention relates to medicinal liquor, in particular to medicinal liquor for treating gout.

Background

With the development of social economy, the dietary structure and the life style of people are greatly changed, the prevalence rate of gout (gout) shows a gradually rising trend, and the gout poses more and more threats to the health of people. Gout is characterized clinically by hyperuricemia and crystalline deposits of urate in connective tissues, particularly cartilage and synovium, and gouty arthritis caused thereby. At present, allopurinol, probenecid, colchicine, non-steroidal anti-inflammatory drugs and other drugs are common anti-gout drugs in China, and occupy a leading position in the market of the anti-gout drugs in China, but the drugs have serious adverse reactions, particularly have great toxic and side effects on kidneys and related organs and are difficult to take for a long time, so the drugs are limited to a certain extent in clinical application. In recent years, the research of the traditional Chinese medicine in the field of gout resistance is gradually increased, compared with western medicines, the traditional Chinese medicine treatment has the remarkable advantages of low recurrence rate after healing, less side effects, no drug resistance in long-term application and the like, and the anti-gout traditional Chinese medicine with definite research and development pharmacological effects, exact curative effects and higher significant efficiency has very attractive prospect.

Disclosure of Invention

The invention aims to provide medicinal liquor for treating gout, which has the effects of reducing uric acid, resisting inflammation, relieving swelling and pain and the like.

In order to achieve the purpose, the invention is realized by the following technical scheme:

a medicinal liquor for treating gout is prepared from the following raw materials in parts by weight: 15-20 parts of plutella xylostella, 1-2 parts of ephedra, 1-2 parts of cassia twig and 1-2 parts of angelica.

Further, the preparation method of the medicinal liquor comprises the following steps:

1) cleaning the above materials, pulverizing, and placing in a porcelain jar or glass jar;

2) adding water which is 1-1.5 times of the total mass of the raw materials and yeast which is 2-6% of the total mass of the raw materials, and fermenting for 1-3 days at a constant temperature of 20-25 ℃;

3) after the fermentation is finished, frying the mixture with slow fire to slightly scorched and drying the mixture;

4) adding 90-130 parts of 40-70% (v/v) Chinese liquor, sealing, and standing for 15-20 days.

The medicinal mechanism of the invention is as follows: wherein the plutella xylostella hance has the effects of reducing uric acid, relieving swelling and pain, and is used as a main drug in the prescription; the ephedra and the cassia twig dispel wind-damp and relax tendons and collaterals; dang Gui can activate blood and resolve stasis. The medicines are used together to achieve the effects of reducing uric acid, dispelling wind and removing dampness, and relieving swelling and pain.

The invention has the advantages that:

the medicinal liquor is prepared according to the theory of traditional Chinese medicine, and has the effects of reducing uric acid, dispelling wind and removing dampness, and relieving swelling and pain; the invention is prepared by soaking in white spirit, improves the leaching rate of effective components by utilizing the permeability of ethanol, fully exerts the guiding function of the wine and assists the medicinal property of the wine; the invention adopts the folk medicine plutella xylostella as the monarch drug, and has great significance for developing and utilizing plutella xylostella plant resources in China.

Drawings

The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention.

FIG. 1 shows joint swelling indexes of 4 h, 8 h, 24 h, 48 h and 72 h after model building of rats in each group;

FIG. 2 is a graph showing the serum SOD levels in rats of each group;

FIG. 3 is a schematic representation of MDA levels in serum of rats in each group;

FIG. 4 is a graph showing the serum IL-1 β levels in rats of each group;

FIG. 5 is a schematic representation of TGF- β levels in the serum of rats in each group;

FIG. 6 is a graph showing the serum IL-4 levels in rats of each group.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The invention is developed by combining long-term clinical practice experience according to the traditional Chinese medicine theory and the modern medical research result, and the following embodiments are adopted:

example 1

A medicated liquor for treating gout is prepared by taking 1500 g of cyrtotrachelossis macrophylla, 100g of ephedra, 100g of cassia twig and 100g of angelica as raw materials, adding 1800g of water and 40g of yeast, and fermenting at constant temperature of 20-25 ℃ for 1-3 days; after the fermentation is finished, frying the mixture with slow fire to slightly scorched and drying the mixture; adding 40 degree Chinese liquor 6.5L, sealing, soaking for 30 days, and filtering.

Example 2

A medicated liquor for treating gout is prepared by taking 1000g of large fruit moth vine, 50g of ephedra herb, 50g of cassia twig and 50g of Chinese angelica as raw materials, adding 1700g of water and 65g of yeast, and fermenting at constant temperature of 20-25 ℃ for 1-3 days; after fermentation, stir-frying with slow fire to slightly scorched, drying, adding 5.75L of 40 degree Chinese liquor, sealing and soaking for 40 days, and filtering to obtain the final product.

Acute toxicity test of mice

Purpose of the experiment: the acute toxic reaction of mice receiving the medicinal liquor is observed, the toxic reaction possibly occurring in human bodies is analyzed, and reference information is provided for clinical dosage selection.

Experimental animals and breeding management: ICR mice weight 18-22 g, half female and half male, provided by the laboratory animal center of the university of transport, Xian, under the certification number SCXK 2012-003. The room temperature is 20-24 ℃, the relative humidity is 40% -70%, and the food is eaten by freely feeding water.

Selecting the medicines: the tested medicine is the medicinal liquor prepared in example 1; the clinical administration dosage is oral administration, 10 mL each time and three times a day; in the experiment, the daily dosage of human is converted into the dosage of mice, and 0.05 mL/10 g of the drug is administered once.

The experimental method comprises the following steps: 40 ICR mice were divided into a normal control group and a drug administration group. The administration is carried out after fasting for 12 hours, the administration volume of the administration group is 0.05 mL/10 g, and the normal control group is administered with the same dose of physiological saline once. Mice were observed for behavioral activity, status, diet, stool and urine, hair color and secretions after dosing. After 24 hours, 10 mice were dissected from the administration group and necropsied. The remaining mice were observed continuously for one week.

The experimental results are as follows: after administration, 20 mice had no abnormal appearance signs within 24 hours, had free movement, had normal food intake and water intake, had wet granular feces, and had smooth and clean fur. After one week of continuous observation, no abnormality was observed compared with the normal control group, and all mice survived. Abnormal liquid is not found in the chest cavity and the abdominal cavity of the mouse through dissection, flatulence is not found in intestinal canals, color and form abnormality is not found in important organs such as heart, liver, spleen, lung and kidney, bleeding spots or other pathological changes do not exist, the color of gastric mucosa is ruddy, bleeding spots or ulcer do not exist, and abnormality does not exist in autopsy.

And (4) experimental conclusion: according to the clinical administration amount of human, no acute toxicity reaction of the medicinal liquor of the invention is observed.

Second, drug efficacy test

Selecting the medicines: in this experimental example, the medicated wine prepared in example 1 was used for experiments to observe the effect of the medicated wine provided by the present invention on acute gouty arthritis of rats; the dose of administration was 2 times as large as the amount of clinical administration in humans in the high dose group.

Grouping and administration: 60 male SD rats, randomly divided into a normal group, a model group, a colchicine group (1.5 mg/kg), a medicated wine high dose group (7 mL/kg), a medium dose group (3.5 mL/kg) and a low dose group (1.75 mL/kg), were subjected to intragastric administration, and administered 1 time per day.

Molding: adding 250 mg of sodium urate into 10 mL of physiological saline, and carrying out ultrasonic treatment for 30 minutes to prepare sodium urate suspension. After each group of rats is administrated for 5 days, 0.2 mL of sodium urate suspension is injected into an acute gouty arthritis model group at one time by a sterile operation method, an included angle of 45 degrees is formed between the upper front side of a needle mouth inclined plane of a No. 6 needle head and a tibia, a colchicine positive drug control group and rats in a low, medium and high dosage medicinal liquor group are injected into tibioarticular cavities stepped in the right hind feet, 0.2 mL of physiological saline is injected into the same position of the rats in a normal group, and each animal is injected for only 1 time. The normal group and the model group were subjected to intragastric administration with the same amount of physiological saline, and the other groups were subjected to intragastric administration with the corresponding drugs.

Observation indexes and detection indexes are that the circumferential length value of 0.5 mm below the right hind foot treaded joint of each group of rats is measured by using a 2-3 mm wide paper strip and a stainless steel surface caliper at four times at 4 h, 8 h, 24 h, 48 h and 72 h after the model is made, the joint swelling index = (the joint circumferential diameter-initial circumferential diameter at the measuring time point)/initial circumferential diameter multiplied by 100%, after 72 h of administration, chloral hydrate is used for anesthesia, the abdominal aorta is used for taking blood, after serum is separated, the kit is used for detecting the contents of SOD, MDA, IL-1 β, TGF- β and IL-4 in the serum.

As a result:

the effect results of the medicinal liquor with different dosages on the ankle joint swelling of the rats with acute gouty arthritis are shown in figure 1, and compared with a normal group, the joint swelling index of the rats in a model group is obviously increased within 72 hours after model building. Compared with the model group, the ankle swelling index of the high and medium dose medicated wine group is obviously reduced within 8-72 h (P is less than 0.05 or P is less than 0.01).

The influence of the medicinal liquor on the serum SOD, MDA, IL-1 β, TGF- β and IL-4 of the rats with acute gouty arthritis is shown in figure 2, figure 3, figure 4 and figure 5. compared with the model group, the serum SOD, TGF- β and IL-4 of the rats with high and medium dosage of the medicinal liquor are obviously increased, and the MDA and IL-1 β are obviously reduced (P is less than 0.05 or P is less than 0.01).

And (4) conclusion: the results show that the medicinal liquor provided by the invention can obviously relieve the joint swelling of rats with acute gouty arthritis, and plays a therapeutic role by inhibiting the release of inflammatory factors and resisting lipid peroxidation.

Thirdly, typical medical records:

the application method of the medicinal liquor provided by the invention is oral administration, 10 mL of the medicinal liquor is taken each time, three times a day, 7 days are taken as a treatment course, and the medicinal liquor is taken for 1-6 treatment courses according to the state of an illness.

1. If the male is aged for 32 years, the toe joint pain is caused due to the love of eating high-purine food, examination shows that uric acid crystals are accumulated at the joints, and after the medicinal liquor is taken for 4 treatment courses, the joint pain symptom is completely relieved.

2. After the medicinal liquor is taken for 6 courses of treatment, the pain symptoms are completely relieved, and the uric acid level in the body is remarkably reduced.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (3)

1. A medicinal liquor for treating gout is characterized in that: the feed is prepared from the following raw materials in parts by weight: 15-20 parts of plutella xylostella, 1-2 parts of ephedra, 1-2 parts of cassia twig and 1-2 parts of angelica.

2. The medicated wine as set forth in claim 1, characterized in that the preparation method of the medicated wine is as follows:

1) cleaning the above materials, pulverizing, and placing in a porcelain jar or glass jar;

2) adding water which is 1-1.5 times of the total mass of the raw materials and yeast which is 2-6% of the total mass of the raw materials, and fermenting for 1-3 days at a constant temperature of 20-25 ℃;

3) after the fermentation is finished, frying the mixture with slow fire to slightly scorched and drying the mixture;

4) adding 90-130 parts of 40-70% (v/v) Chinese liquor, sealing, and standing for 15-20 days.

3. The use of the medicated wine of claim 1 in the preparation of a medicament for the treatment of gout.

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