CN110892937A - Soybean peptide powder and production process thereof - Google Patents
Soybean peptide powder and production process thereof Download PDFInfo
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- CN110892937A CN110892937A CN201910632334.3A CN201910632334A CN110892937A CN 110892937 A CN110892937 A CN 110892937A CN 201910632334 A CN201910632334 A CN 201910632334A CN 110892937 A CN110892937 A CN 110892937A
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- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 29
- 235000010469 Glycine max Nutrition 0.000 title claims abstract description 28
- 244000068988 Glycine max Species 0.000 title claims abstract description 28
- 239000000843 powder Substances 0.000 title claims abstract description 28
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 19
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims abstract description 42
- 239000012528 membrane Substances 0.000 claims abstract description 33
- 108091005804 Peptidases Proteins 0.000 claims abstract description 31
- 239000004365 Protease Substances 0.000 claims abstract description 31
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 31
- 235000019764 Soybean Meal Nutrition 0.000 claims abstract description 29
- 239000004455 soybean meal Substances 0.000 claims abstract description 29
- 239000007788 liquid Substances 0.000 claims abstract description 27
- 230000001954 sterilising effect Effects 0.000 claims abstract description 26
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 26
- 238000000034 method Methods 0.000 claims abstract description 24
- 150000001875 compounds Chemical class 0.000 claims abstract description 20
- 241000894006 Bacteria Species 0.000 claims abstract description 18
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 claims abstract description 14
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 claims abstract description 14
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 claims abstract description 14
- 229960002675 xylitol Drugs 0.000 claims abstract description 14
- 235000010447 xylitol Nutrition 0.000 claims abstract description 14
- 239000000811 xylitol Substances 0.000 claims abstract description 14
- 238000004537 pulping Methods 0.000 claims abstract description 13
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 12
- 238000005374 membrane filtration Methods 0.000 claims abstract description 11
- 108091005658 Basic proteases Proteins 0.000 claims abstract description 8
- 241000196324 Embryophyta Species 0.000 claims abstract description 8
- 108010007119 flavourzyme Proteins 0.000 claims abstract description 8
- 229930003231 vitamin Natural products 0.000 claims abstract description 8
- 235000013343 vitamin Nutrition 0.000 claims abstract description 8
- 239000011782 vitamin Substances 0.000 claims abstract description 8
- 229940088594 vitamin Drugs 0.000 claims abstract description 8
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims abstract description 6
- 229930003268 Vitamin C Natural products 0.000 claims abstract description 6
- 239000002994 raw material Substances 0.000 claims abstract description 6
- 235000019154 vitamin C Nutrition 0.000 claims abstract description 6
- 239000011718 vitamin C Substances 0.000 claims abstract description 6
- 238000001914 filtration Methods 0.000 claims description 30
- 238000010438 heat treatment Methods 0.000 claims description 15
- 239000011259 mixed solution Substances 0.000 claims description 15
- 230000002779 inactivation Effects 0.000 claims description 10
- 239000000243 solution Substances 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 102000004190 Enzymes Human genes 0.000 claims description 9
- 108090000790 Enzymes Proteins 0.000 claims description 9
- 239000000047 product Substances 0.000 claims description 8
- 241000251468 Actinopterygii Species 0.000 claims description 5
- 241001297689 Candidatus Glassbacteria Species 0.000 claims description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 5
- 239000000020 Nitrocellulose Substances 0.000 claims description 5
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 claims description 5
- 239000000920 calcium hydroxide Substances 0.000 claims description 5
- 229910001861 calcium hydroxide Inorganic materials 0.000 claims description 5
- 229910052799 carbon Inorganic materials 0.000 claims description 5
- 238000004140 cleaning Methods 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 5
- 239000008367 deionised water Substances 0.000 claims description 5
- 229910021641 deionized water Inorganic materials 0.000 claims description 5
- 230000000249 desinfective effect Effects 0.000 claims description 5
- 239000000706 filtrate Substances 0.000 claims description 5
- 230000000415 inactivating effect Effects 0.000 claims description 5
- 230000006698 induction Effects 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 5
- 229910052751 metal Inorganic materials 0.000 claims description 5
- 239000002184 metal Substances 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 229920001220 nitrocellulos Polymers 0.000 claims description 5
- 238000004806 packaging method and process Methods 0.000 claims description 5
- 239000010451 perlite Substances 0.000 claims description 5
- 235000019362 perlite Nutrition 0.000 claims description 5
- 238000001179 sorption measurement Methods 0.000 claims description 5
- 238000001694 spray drying Methods 0.000 claims description 5
- 239000006228 supernatant Substances 0.000 claims description 5
- 239000002131 composite material Substances 0.000 claims description 3
- 102000004169 proteins and genes Human genes 0.000 abstract description 11
- 108090000623 proteins and genes Proteins 0.000 abstract description 11
- 102000008186 Collagen Human genes 0.000 abstract description 6
- 108010035532 Collagen Proteins 0.000 abstract description 6
- 229920001436 collagen Polymers 0.000 abstract description 6
- 230000036512 infertility Effects 0.000 abstract description 4
- 230000014759 maintenance of location Effects 0.000 abstract description 4
- 235000015097 nutrients Nutrition 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 235000008452 baby food Nutrition 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/346—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of vegetable proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/001—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from waste materials, e.g. kitchen waste
- A23J1/005—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from waste materials, e.g. kitchen waste from vegetable waste materials
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/14—Vegetable proteins
- A23J3/16—Vegetable proteins from soybean
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/03—Organic compounds
- A23L29/035—Organic compounds containing oxygen as heteroatom
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
- A23L29/37—Sugar alcohols
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Nutrition Science (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Mycology (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses soybean peptide powder and a production process thereof, wherein the soybean peptide powder comprises the following raw materials in parts by weight: 5-15 parts of soybean meal, 1-3 parts of xylitol, 0.3-0.5 part of citric acid and 0.003-0.007 part of vitamin C. The process of the invention adds vitamins, and effectively prevents the protein from being oxidized in the processing process. The added citric acid can adjust the pH of the feed liquid and can prevent the protein from being oxidized in the processing process. Adding xylitol for flavoring; the provided membrane concentration process is combined with the vacuum concentration process to effectively preserve the nutrient components and the aroma of the protein; the set membrane filtration sterilization process is used for carrying out membrane filtration sterilization through a bacteria filter; bacteria in liquid or air are removed by a physical retention method so as to achieve the aim of sterility; the arranged compound protease consists of alkaline protease, plant protease and flavourzyme, and has the effect of stepwise enzymolysis; the soybean meal is finely stirred by combining a high-rotation-speed pulping machine, so that the enzymolysis efficiency is further improved, and the finished collagen is easier to be absorbed by a human body.
Description
Technical Field
The invention relates to soybean peptide powder and a production process thereof.
Background
Compared with the original protein and amino acid preparation with the same components, the soybean peptide powder is easier to be absorbed by human body, can directly enter small intestine to be absorbed without gastrointestinal digestion, and is not influenced by physical condition. The soybean peptide powder contains various bioactive peptides, and has various physiological activities including resisting oxidation, lowering blood pressure, lowering cholesterol, reducing blood lipid, relieving fatigue, and enhancing immunity. As a novel multifunctional nutritional ingredient, the soybean peptide powder can be widely used in health food, infant food, sports food, fermented products, clinical nutritional preparations and the like. The existing soybean peptide powder is easy to oxidize in the production process, and the produced hydrolyzed collagen is not easy to absorb and has poor taste.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide soybean peptide powder and a production process thereof.
The technical scheme adopted by the invention for solving the technical problems is as follows: the soybean peptide powder comprises the following raw materials in parts by weight: 5-15 parts of soybean meal, 1-3 parts of xylitol, 0.3-0.5 part of citric acid and 0.003-0.007 part of vitamin C.
A production process of soybean peptide powder comprises the following steps:
1) checking and accepting raw and auxiliary materials, selecting fresh soybean meal, and then cleaning and disinfecting the soybean meal for later use;
2) adding deionized water into the accepted soybean meal, mixing, pouring into a pulping machine, and pulping to obtain a feed liquid;
3) heating the feed liquid to 98 ℃, adding vitamins, keeping for 15min, and cooling to room temperature;
4) adding the feed liquid into an enzymolysis tank, and adjusting the pH of the feed liquid to 6.5-9.5 by using citric acid;
5) simultaneously adding compound protease, and carrying out enzymolysis for 7h at the constant temperature of 32-67 ℃;
6) inactivating the enzyme by an inactivation device;
7) primary filtering, namely adding perlite and calcium hydroxide and then filtering to obtain filtrate;
8) decolorizing and debitterizing, adding active carbon for adsorption, and separating with a centrifuge to obtain supernatant;
9) decarbonizing, namely decarbonizing the mixed solution through a decarbonizer;
10) fine filtering, and allowing the mixed solution to flow through a fine filter for fine filtering;
11) membrane concentration; the mixed solution flows through membrane concentration equipment for membrane concentration to obtain concentrated solution;
12) vacuum concentration; under the induction of secondary steam and the suction of high vacuum of the separator, the concentrated solution and the secondary steam enter the separator at a higher speed along the tangential direction; the vacuum technology preserves the nutrient components and fragrance of protein.
13) Membrane filtration sterilization; filtering with membrane filter for sterilization, and adding xylitol for flavoring; bacteria in liquid or air are removed by physical retention to achieve the purpose of sterility. The used device is a bacteria filter with tiny pore diameter;
14) spray drying to obtain a finished product;
15) packaging, detecting by a metal detector and warehousing.
Further, the ratio of water to soybean meal in the step 2) is 5: 1.
Further, the refiner rotation speed in step 2) was 17000 rpm.
Further, the total weight of the compound protease in the step 5) accounts for 0.07-1% of the weight of the fish skin.
Further, the bacteria filter in the step 13) is one of a sintered glass bacteria filter, collodion and a nitrocellulose filter membrane.
Further, the filter membrane specification of the bacteria filter in the step 13) is 0.45um and 0.22um, the filtering pressure is less than 0.25MPa, and the flow is less than 200L/h.
Further, the compound protease comprises alkaline protease, plant protease and flavourzyme, and the weight ratio of the components is 2: 1: 1-4: 3: 1.
further, the inactivation device in the step 6) is one of an ultraviolet lamp or a high-temperature sterilization pot.
Further, when the compound protease is inactivated by a high-temperature sterilization pot, in order to ensure that the enzyme is inactivated fully, the heating temperature is 65-100 ℃, and the heating time can be kept for 10-25 minutes.
The invention has the beneficial effects that: the process of the invention adds vitamins, and effectively prevents the protein from being oxidized in the processing process. The added citric acid can adjust the pH of the feed liquid and can prevent the protein from being oxidized in the processing process. The added xylitol can be used for flavoring.
The membrane concentration process provided by the process of the invention is combined with the vacuum concentration process to effectively preserve the nutrient components and aroma of the protein.
According to the membrane filtration sterilization process provided by the invention, membrane filtration sterilization is carried out through a bacteria filter; bacteria in liquid or air are removed by physical retention to achieve the purpose of sterility.
The composite protease set by the process consists of alkaline protease, plant protease and flavourzyme, and has the effect of stepwise enzymolysis. The soybean meal is finely stirred by combining a high-rotation-speed pulping machine, so that the enzymolysis efficiency is further improved. The molecular weight of the collagen of the finished product is smaller, and the collagen is easier to be absorbed by a human body.
Detailed Description
The present invention is further described with reference to specific examples to enable those skilled in the art to better understand the present invention and to practice the same, but the examples are not intended to limit the present invention.
Example 1
The soybean peptide powder comprises the following raw materials in parts by weight: 5 parts of soybean meal, 1 part of xylitol, 0.3 part of citric acid and 0.003 part of vitamin C.
A production process of soybean peptide powder comprises the following steps:
1) checking and accepting raw and auxiliary materials, selecting fresh soybean meal, and then cleaning and disinfecting the soybean meal for later use;
2) adding deionized water into the accepted soybean meal, mixing, pouring into a pulping machine, and pulping to obtain a feed liquid;
3) heating the feed liquid to 98 ℃, adding vitamins, keeping for 15min, and cooling to room temperature;
4) adding the feed liquid into an enzymolysis tank, and adjusting the pH of the feed liquid to 6.5 by using citric acid;
5) simultaneously adding compound protease, and carrying out enzymolysis for 7h at the constant temperature of 32 ℃;
6) inactivating the enzyme by an inactivation device;
7) primary filtering, namely adding perlite and calcium hydroxide and then filtering to obtain filtrate;
8) decolorizing and debitterizing, adding active carbon for adsorption, and separating with a centrifuge to obtain supernatant;
9) decarbonizing, namely decarbonizing the mixed solution through a decarbonizer;
10) fine filtering, and allowing the mixed solution to flow through a fine filter for fine filtering;
11) membrane concentration; the mixed solution flows through membrane concentration equipment for membrane concentration to obtain concentrated solution;
12) vacuum concentration; under the induction of secondary steam and the suction of high vacuum of the separator, the concentrated solution and the secondary steam enter the separator at a higher speed along the tangential direction;
13) membrane filtration sterilization; filtering with membrane filter for sterilization, and adding xylitol for flavoring;
14) spray drying to obtain a finished product;
15) packaging, detecting by a metal detector and warehousing.
Further, the ratio of water to soybean meal in the step 2) is 5: 1.
Further, the refiner rotation speed in step 2) was 17000 rpm.
Further, the total weight of the compound protease in the step 5) accounts for 0.07 percent of the weight of the fish skin.
Further, the bacteria filter in the step 13) is one of a sintered glass bacteria filter, collodion and a nitrocellulose filter membrane.
Further, the filter membrane specification of the bacteria filter in the step 13) is 0.45um and 0.22um, the filtering pressure is less than 0.25MPa, and the flow is less than 200L/h.
Further, the compound protease comprises alkaline protease, plant protease and flavourzyme, and the weight ratio of the components is 2: 1: 1.
further, the inactivation device in the step 6) is one of an ultraviolet lamp or a high-temperature sterilization pot.
Further, when the compound protease is inactivated by a high-temperature sterilization pot, the heating temperature is 65 ℃ and the heating time can be kept for 10 minutes in order to ensure that the enzyme is fully inactivated.
Example 2
The soybean peptide powder comprises the following raw materials in parts by weight: 15 parts of soybean meal, 3 parts of xylitol, 0.5 part of citric acid and 0.007 part of vitamin C.
A production process of soybean peptide powder comprises the following steps:
1) checking and accepting raw and auxiliary materials, selecting fresh soybean meal, and then cleaning and disinfecting the soybean meal for later use;
2) adding deionized water into the accepted soybean meal, mixing, pouring into a pulping machine, and pulping to obtain a feed liquid;
3) heating the feed liquid to 98 ℃, adding vitamins, keeping for 15min, and cooling to room temperature;
4) adding the feed liquid into an enzymolysis tank, and adjusting the pH of the feed liquid to 9.5 by using citric acid;
5) simultaneously adding compound protease, and carrying out enzymolysis for 7h at the constant temperature of 67 ℃;
6) inactivating the enzyme by an inactivation device;
7) primary filtering, namely adding perlite and calcium hydroxide and then filtering to obtain filtrate;
8) decolorizing and debitterizing, adding active carbon for adsorption, and separating with a centrifuge to obtain supernatant;
9) decarbonizing, namely decarbonizing the mixed solution through a decarbonizer;
10) fine filtering, and allowing the mixed solution to flow through a fine filter for fine filtering;
11) membrane concentration; the mixed solution flows through membrane concentration equipment for membrane concentration to obtain concentrated solution;
12) vacuum concentration; under the induction of secondary steam and the suction of high vacuum of the separator, the concentrated solution and the secondary steam enter the separator at a higher speed along the tangential direction;
13) membrane filtration sterilization; filtering with membrane filter for sterilization, and adding xylitol for flavoring;
14) spray drying to obtain a finished product;
15) packaging, detecting by a metal detector and warehousing.
Further, the ratio of water to soybean meal in the step 2) is 5: 1.
Further, the refiner rotation speed in step 2) was 17000 rpm.
Further, the total weight of the compound protease in the step 5) accounts for 1% of the weight of the fish skin.
Further, the bacteria filter in the step 13) is one of a sintered glass bacteria filter, collodion and a nitrocellulose filter membrane.
Further, the filter membrane specification of the bacteria filter in the step 13) is 0.45um and 0.22um, the filtering pressure is less than 0.25MPa, and the flow is less than 200L/h.
Further, the compound protease comprises alkaline protease, plant protease and flavourzyme, and the weight ratio of the components is 4: 3: 1.
further, the inactivation device in the step 6) is one of an ultraviolet lamp or a high-temperature sterilization pot.
Further, when the compound protease is inactivated by a high-temperature sterilization pot, the heating temperature is 100 ℃ and the heating time can be kept for 25 minutes in order to ensure that the enzyme is fully inactivated.
Example 3
The soybean peptide powder comprises the following raw materials in parts by weight: 10 parts of soybean meal, 2 parts of xylitol, 0.4 part of citric acid and 0.005 part of vitamin C.
A production process of soybean peptide powder comprises the following steps:
1) checking and accepting raw and auxiliary materials, selecting fresh soybean meal, and then cleaning and disinfecting the soybean meal for later use;
2) adding deionized water into the accepted soybean meal, mixing, pouring into a pulping machine, and pulping to obtain a feed liquid;
3) heating the feed liquid to 98 ℃, adding vitamins, keeping for 15min, and cooling to room temperature;
4) adding the feed liquid into an enzymolysis tank, and adjusting the pH of the feed liquid to 8 by using citric acid;
5) simultaneously adding compound protease, and carrying out enzymolysis for 7h at a constant temperature of 50 ℃;
6) inactivating the enzyme by an inactivation device;
7) primary filtering, namely adding perlite and calcium hydroxide and then filtering to obtain filtrate;
8) decolorizing and debitterizing, adding active carbon for adsorption, and separating with a centrifuge to obtain supernatant;
9) decarbonizing, namely decarbonizing the mixed solution through a decarbonizer;
10) fine filtering, and allowing the mixed solution to flow through a fine filter for fine filtering;
11) membrane concentration; the mixed solution flows through membrane concentration equipment for membrane concentration to obtain concentrated solution;
12) vacuum concentration; under the induction of secondary steam and the suction of high vacuum of the separator, the concentrated solution and the secondary steam enter the separator at a higher speed along the tangential direction;
13) membrane filtration sterilization; filtering with membrane filter for sterilization, and adding xylitol for flavoring;
14) spray drying to obtain a finished product;
15) packaging, detecting by a metal detector and warehousing.
Further, the ratio of water to soybean meal in the step 2) is 5: 1.
Further, the refiner rotation speed in step 2) was 17000 rpm.
Further, the total weight of the compound protease in the step 5) accounts for 0.6% of the weight of the fish skin.
Further, the bacteria filter in the step 13) is one of a sintered glass bacteria filter, collodion and a nitrocellulose filter membrane.
Further, the filter membrane specification of the bacteria filter in the step 13) is 0.45um and 0.22um, the filtering pressure is less than 0.25MPa, and the flow is less than 200L/h.
Further, the compound protease comprises alkaline protease, plant protease and flavourzyme, and the weight ratio of the components is 2: 1: 1.
further, the inactivation device in the step 6) is one of an ultraviolet lamp or a high-temperature sterilization pot.
Further, when the compound protease is inactivated by a high-temperature sterilization pot, the heating temperature is 80 ℃ and the heating time can be kept for 15 minutes in order to ensure that the enzyme is inactivated sufficiently.
The invention has the beneficial effects that: the process of the invention adds vitamins, and effectively prevents the protein from being oxidized in the processing process. The added citric acid can adjust the pH of the feed liquid and can prevent the protein from being oxidized in the processing process. The added xylitol can be used for flavoring.
The membrane concentration process provided by the process of the invention is combined with the vacuum concentration process to effectively preserve the nutrient components and aroma of the protein.
According to the membrane filtration sterilization process provided by the invention, membrane filtration sterilization is carried out through a bacteria filter; bacteria in liquid or air are removed by physical retention to achieve the purpose of sterility. The apparatus used is a bacterial filter with a tiny pore diameter.
The composite protease set by the process consists of alkaline protease, plant protease and flavourzyme, and has the effect of stepwise enzymolysis. The soybean meal is finely stirred by combining a high-rotation-speed pulping machine, so that the enzymolysis efficiency is further improved, and the molecular weight of the collagen of the finished product is smaller and the collagen is easier to be absorbed by a human body.
The above-described embodiments of the present invention are not intended to limit the scope of the present invention, and the embodiments of the present invention are not limited thereto, and various other modifications, substitutions and alterations can be made to the above-described structure of the present invention without departing from the basic technical concept of the present invention as described above, according to the common technical knowledge and conventional means in the field of the present invention.
Claims (10)
1. A soybean peptide powder is characterized in that: the weight ratio of each raw material is as follows: 5-15 parts of soybean meal, 1-3 parts of xylitol, 0.3-0.5 part of citric acid and 0.003-0.007 part of vitamin C.
2. A production process of soybean peptide powder is characterized by comprising the following steps: the method comprises the following steps:
1) checking and accepting raw and auxiliary materials, selecting fresh soybean meal, and then cleaning and disinfecting the soybean meal for later use;
2) adding deionized water into the accepted soybean meal, mixing, pouring into a pulping machine, and pulping to obtain a feed liquid;
3) heating the feed liquid to 98 ℃, adding vitamins, keeping for 15min, and cooling to room temperature;
4) adding the feed liquid into an enzymolysis tank, and adjusting the pH of the feed liquid to 6.5-9.5 by using citric acid;
5) simultaneously adding compound protease, and carrying out enzymolysis for 7h at the constant temperature of 32-67 ℃;
6) inactivating the enzyme by an inactivation device;
7) primary filtering, namely adding perlite and calcium hydroxide and then filtering to obtain filtrate;
8) decolorizing and debitterizing, adding active carbon for adsorption, and separating with a centrifuge to obtain supernatant;
9) decarbonizing, namely decarbonizing the mixed solution through a decarbonizer;
10) fine filtering, and allowing the mixed solution to flow through a fine filter for fine filtering;
11) membrane concentration; the mixed solution flows through membrane concentration equipment for membrane concentration to obtain concentrated solution;
12) vacuum concentration; under the induction of secondary steam and the suction of high vacuum of the separator, the concentrated solution and the secondary steam enter the separator at a higher speed along the tangential direction;
13) membrane filtration sterilization; filtering with membrane filter for sterilization, and adding xylitol for flavoring;
14) spray drying to obtain a finished product;
15) packaging, detecting by a metal detector and warehousing.
3. The soybean peptide powder and the production process thereof as claimed in claim 1, wherein: the ratio of water to soybean meal in step 2) is 5: 1.
4. The soybean peptide powder and the production process thereof as claimed in claim 1, wherein: the refiner rotation speed in step 2) was 17000 revolutions per minute.
5. The soybean peptide powder and the production process thereof as claimed in claim 1, wherein: the total weight of the compound protease in the step 5) accounts for 0.07-1% of the weight of the fish skin.
6. The soybean peptide powder and the production process thereof as claimed in claim 1, wherein: the bacteria filter in the step 13) is one of a sintered glass bacteria filter, collodion and a nitrocellulose filter membrane.
7. The soybean peptide powder and the production process thereof as claimed in claim 1, wherein: the filter membrane specification of the bacteria filter in the step 13) is 0.45um and 0.22um, the filtering pressure is less than 0.25MPa, and the flow is less than 200L/h.
8. The soybean peptide powder and the production process thereof as claimed in claim 1, wherein: the compound protease consists of alkaline protease, plant protease and flavourzyme, and the weight ratio of the components is 2: 1: 1-4: 3: 1.
9. the soybean peptide powder and the production process thereof as claimed in claim 2, wherein: the inactivation equipment in the step 6) is one of an ultraviolet lamp or a high-temperature sterilization pot.
10. The soybean peptide powder and the production process thereof as claimed in claim 8, wherein: when the composite protease is inactivated by a high-temperature sterilization pot, the heating temperature is 65-100 ℃, and the heating time can be kept for 10-25 minutes.
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