CN110859790B

CN110859790B - Application of traditional Chinese medicine composition in preparation of oral care product

Info

Publication number: CN110859790B
Application number: CN201911394770.8A
Authority: CN
Inventors: 胡永志; 李江平; 唐红艳; 谭耀政; 黄晓燕; 杨卫豪; 韦日平
Original assignee: LIUZHOU LMZ CO Ltd
Current assignee: LIUZHOU LMZ CO Ltd
Priority date: 2019-12-30
Filing date: 2019-12-30
Publication date: 2022-06-24
Anticipated expiration: 2039-12-30
Also published as: CN110859790A

Abstract

The invention discloses an application of a traditional Chinese medicine composition in preparing an oral care product, relating to the technical field of oral care, namely, in the process of preparing the oral care product, the traditional Chinese medicine composition is used as the only active ingredient with anti-inflammatory and analgesic effects to be added into the oral care product, the traditional Chinese medicine composition comprises artemisinin, radix zanthoxyli extracts and sodium copper chlorophyllin, the artemisinin accounts for 0.001-3.0 wt% of the raw materials in preparing the oral care product, the radix zanthoxyli extracts accounts for 0.1-5.0 wt% of the raw materials in preparing the oral care product, the sodium copper chlorophyllin accounts for 0.1-5.0 wt% of the raw materials in preparing the oral care product, compared with the prior art, the artemisinin, the radix zanthoxyli and the sodium copper chlorophyllin are added as the raw materials of the active ingredients in the preparation process of the oral care product, so that the use is safe, but also has obvious anti-inflammatory and analgesic effects.

Description

Application of traditional Chinese medicine composition in preparation of oral care product

Technical Field

The invention relates to the technical field of oral care, in particular to an oral care product with hemostatic and analgesic effects.

Background

The oral care products are indispensable cleaning products in daily life of people, and the products have various types, including toothpaste, mouthwash, tooth powder, chewing gum and the like, and are mainly used for keeping the oral cavity clean, promoting breath freshening, nursing gingiva, maintaining good environment of the oral cavity and the like.

Gingival inflammation is one of the common symptoms in the stomatology department. Gingivitis typically results in red, swollen, painful and even bleeding gums. Gingivitis is of many types, but the most common and high incidence is chronic simple gingivitis, also known as dirty gingivitis and marginal gingivitis, which is commonly referred to as chronic simple gingivitis. The common clinical methods are to clean and remove calculus on the tooth body, and to take oral anti-inflammatory drugs to relieve gingival inflammation. Therefore, prevention and treatment of gingival inflammation has become a focus of attention of oral clinicians in recent years, and is also favored by manufacturers of oral health products because of its great commercial value. Therefore, toothpaste with various functional types in oral care products at home and abroad becomes the mainstream of the market, and the functional oral care products with the traditional Chinese medicine active ingredients in the market become the key point of the current development because the traditional Chinese medicine plant active ingredients have the advantages of naturalness, safety and the like which are incomparable with western medicines. Therefore, the development of a safe and effective Chinese herbal medicine toothpaste aiming at the oral cavity problem of treating the gum swelling and pain is imperative, and the concept and the requirement of modern human beings on natural green environmental protection are also met.

Disclosure of Invention

The invention solves the problem of providing an application scheme of the traditional Chinese medicine composition in preparing oral care products, and the oral care products have obvious anti-inflammatory and analgesic effects.

The application scheme of the traditional Chinese medicine composition in preparing the oral care product is that the traditional Chinese medicine composition is used as the only active component with the anti-inflammatory and analgesic effects to be added into the oral care product in the process of preparing the oral care product, the traditional Chinese medicine composition comprises artemisinin, radix zanthoxyli and sodium copper chlorophyllin, the artemisinin accounts for 0.001-3.0% of the raw materials in the process of preparing the oral care product, the radix zanthoxyli extract accounts for 0.1-5.0% of the raw materials in the process of preparing the oral care product, and the sodium copper chlorophyllin accounts for 0.1-5.0% of the raw materials in the process of preparing the oral care product.

In the above technical solution, a more specific solution may also be: the traditional Chinese medicine composition is used as the only active component with the functions of diminishing inflammation and easing pain in the process of preparing the toothpaste.

In the scheme, the traditional Chinese medicine composition is used as the only active ingredient with the anti-inflammatory and analgesic effects in the process of preparing the mouthwash.

In the scheme, the traditional Chinese medicine composition is used as the only active ingredient with the anti-inflammatory and analgesic effects in the process of preparing the dentifrice.

In the scheme, the traditional Chinese medicine composition is used as the only active ingredient with the anti-inflammatory and analgesic effects in the process of preparing the chewing gum.

The sweet wormwood is the dry aerial part of Artemisia annua L of Compositae. Harvesting in autumn when the flower is full, removing old stem, and drying in shade. The book of Chinese pharmacopoeia 2015 edition records that sweet wormwood is bitter, pungent and cold. It enters liver and gallbladder meridians. Clear deficiency-type heat, remove bone-steaming, relieve summer-heat, check malaria, and relieve jaundice. Can be used for treating yin impairment, early cooling, fever due to yin deficiency, hectic fever due to yin deficiency, fever due to summer-heat pathogen, malaria, and jaundice due to heat. The first herb of southernwood is found in Shennong Ben Cao Jing, listed as the following article, and recorded in the book: "itching scab, sore, louse, retention of heat between the bone segments, vision improvement". The main component of the sweet wormwood herb is artemisinin, and the artemisinin shows certain antibacterial activity due to the antifungal effect of the artemisinin. Researches prove that the residue powder and the decoction of the artemisinin have strong bacteriostatic action on bacillus anthracis, staphylococcus epidermidis, catacoccus catarrhalis and diphtheria bacillus, and also have certain bacteriostatic action on tubercle bacillus, pseudomonas aeruginosa, staphylococcus aureus, shigella dysenteriae and the like.

The Zanthoxylum nitidum is dry root of Zanthoxylum nitidum (Roxb.) DC. of Rutaceae, and has bitter and pungent taste and mild property. Has effects of promoting blood circulation for removing blood stasis, activating qi-flowing, relieving pain, dispelling pathogenic wind, dredging collaterals, removing toxic substance and detumescence, and can be used for treating traumatic injury, stomach ache, toothache, rheumatalgia, etc. The Zanthoxylum nitidum, Zanthoxylum piperitum, and Zanthoxylum nitidum, is first recorded in Shennong Ben Cao Jing, listed as the following article, and recorded in the book: the fructus zanthoxyli is bitter in taste and warm in nature, and is mainly used for treating wind-cold-dampness arthralgia, joint pain, limb original qi removal and knee pain. The formula of the medicine is that the medicine is prepared from Chinese medicinal herbs including Ben Cao gang mu, Ling nan Cao Yao Fang Feng and she Chuan, Chi Shu is used for treating traumatic injury, snake bite, toothache, gargling, Hu nan Yao Zhi, Hu Na Yao Zhi is used for treating pain, swelling and pain of throat caused by biliary ascariasis, and skin mucosa anesthesia, and Lu Chuan Yao is used for treating neuralgia, gastric ulcer, duodenal ulcer, gastrointestinal colic, and biliary ascariasis. It is used to treat traumatic fracture, swelling and pain due to injury, rheumatic osteodynia, pain of heart and stomach, and toothache. And treat snake bite. In folk, proper amount of radix Zanthoxyli is taken, and decocted with water or ground into powder for oral administration, which is used for treating headache, toothache, etc. The radix zanthoxyli extract mainly comprises new rod-shaped zanthoxylum bungeanum amide and nitidine chloride, and Chinese pharmacopoeia (2015 edition) records that radix zanthoxyli has the effects of promoting diuresis, clearing away heat and toxic materials, and dispersing tinea and reducing swelling. Can be used for treating heat stranguria, stranguria with stone, heat jaundice, skin sore, carbuncle, and traumatic injury.

The sodium copper chlorophyllin is derived from natural substances and has the effects of resisting inflammation, promoting wound healing and recovering the physiological functions of periodontal tissues and alveolar bones.

The extraction method of the traditional Chinese medicine comprises the following steps: the artemisinin is obtained by extracting and purifying sweet wormwood herb, and is a pure product with the content of 98 percent; the radix Zanthoxyli extraction method comprises crushing radix Zanthoxyli into certain particle size, extracting with 10 times of ethanol under reflux for 3 hr for 3 times, and concentrating the extractive solution under reduced pressure to obtain extract or drying to obtain powder. The method for extracting radix Zanthoxyli is not limited to the above method, and other methods such as supercritical extraction and ultrasonic extraction can be used for extraction; the sodium copper chlorophyllin is one of chlorophyll derivatives, is prepared from chlorophyll as raw material by extracting, separating, concentrating, saponifying, salinizing and purifying, and has a purity of 98.5%.

The extraction method of the above traditional Chinese medicines is not limited to the above method, and other methods such as supercritical extraction and ultrasonic extraction can be adopted for extraction.

The traditional Chinese medicine composition is added in the preparation process of the oral care product as a raw material of an active ingredient, so that the oral care product is safe to use and has obvious anti-inflammatory and analgesic effects.

Detailed Description

The invention is further illustrated by the following examples in which the percentages are by weight unless otherwise indicated.

The toothpaste composition raw materials and the weight percentage content in the examples 1 to 8 are shown in the table 1:

the preparation method of the toothpaste in example 1:

the toothpaste body can be prepared by adopting a one-step toothpaste preparation production process of the conventional medicinal toothpaste: adding measured liquid materials such as purified water and Chinese medicinal composition into a temporary storage tank; adding the weighed calcite powder, saccharin sodium, sodium carboxymethylcellulose, sodium dodecyl sulfate, sodium paraben and other powder materials into a pre-dispersion kettle, and performing pre-dispersion and mixing; opening a paste preparation kettle and sucking the liquid material by a vacuum pump; then, a scraper is opened, the powder is fed, a stirrer and a colloid mill are opened, essence is put in after 50 minutes, the colloid mill, the stirrer and the scraper are closed in sequence after 10 minutes, vacuum is broken, a vacuum pump is stopped, and sampling detection is qualified.

Preparation of toothpaste according to examples 2-9 reference is made to example 1.

The mouth rinses of examples 10 to 18 comprise the following raw materials in percentage by weight as shown in table 2:

the preparation of the mouthwashes of examples 9-18:

adding the traditional Chinese medicine composition and the nipagin mixed ester sodium into edible ethanol, stirring and dissolving the mixture, placing the mixture in a temporary storage pot, adding the sorbitol, the deionized and the hydrogenated castor oil-40 into the stirring pot, stirring the mixture for 10 minutes, adding the ethanol solution in the temporary storage pot into the stirring pot, stirring the mixture for 10 minutes, adding the essence, stirring the mixture for 5 minutes, stopping stirring, and sampling and detecting the mixture to be qualified.

The dentifrice compositions and weight percentages of the dentifrice compositions of examples 19 to 27 are as shown in table 3:

the preparation of the dentifrices of examples 19, 20, 21, 22, 23, 24, 25, 26 and 27:

weighing natural calcium carbonate, sodium carboxymethylcellulose, sodium dodecyl sulfate and saccharin sodium according to the formula dosage, adding into a mixing stirrer, stirring for 20 minutes, adding the traditional Chinese medicine composition, stirring for 10 minutes, finally adding essence and sorbitol, stirring for 20 minutes, stopping the machine, and sampling to detect to be qualified.

The chewing gum of examples 28 to 32 comprises the following raw materials in percentage by weight as shown in table 4:

the preparation process of examples 28 to 36 uses existing chewing gum production processes.

Pharmacodynamic test part:

1. analgesia experiment

1.1, sample:

adding traditional Chinese medicine formula toothpaste containing artemisinin, radix zanthoxyli and sodium copper chlorophyllin (examples 1-8), blank toothpaste matrix, defluorinated diethylamine group and blank control (normal saline).

1.2, the method and the process are as follows:

selecting 110 healthy Kunming white mice weighing 16-25g, adjusting the temperature to 55 +/-0.5 ℃ by using a GJ-8402 hot plate pain tester, and placing the white mice on a hot plate to measure the pain threshold value of each mouse. The test article was randomly divided into 11 groups, and each group 10 was applied to the toes of the foot with the corresponding test article. The interval is 15min each time, the threshold of mice is measured 15min, 30min, 45min after the test object is applied, and the measurement is repeated 2 times in the same time period. If the mice still have no response for 60s, the mice are taken out to avoid scalding, and the pain threshold is calculated as 60 s. The pain threshold of each group of mice was recorded and the data were subjected to statistical T-test with results as shown in table 5:

as a result:

experimental results show that the groups of examples 1-4 and the group of the difluorofenac diethylamine can obviously improve the pain threshold of the white mouse compared with the group before administration and a blank control group, and have obvious analgesic effect; examples 5-9, the analgesic effect was superior to that of the blank control group, but lower than that of examples 1-4; examples 5-9 and the blank control group did not show significant analgesic effect, indicating that one of the traditional Chinese medicine compositions is lacking or one of the compositions has a value lower or higher than that of the present invention, and the analgesic effect is not significant.

Anti-inflammatory assay

The method and the process are as follows:

selecting 110 healthy Kunming mice, randomly dividing into 11 groups of 10 mice.

Blank control group (physiological saline 0.1 ml/piece), fluocinonide light pine cream group (fluocinonide 0.1 ml/piece), toothpaste sample group (example 1-example 8 sample 0.1 ml/piece).

In the experiment, 0.1ml of dimethylbenzene is smeared on the inner and outer surfaces of the right ear corridor of each animal in each group until inflammation occurs, and the left ear is not treated to be used as a self blank control group. After being coated with dimethylbenzene for 30min, each group of animals is respectively and uniformly coated with 0.1g of test substance sample on the inner surface and the outer surface of the right ear corridor of each group of animals. After the test object is given for 1h, the white mouse is killed by dislocation of the cervical vertebra, the two ear galleries are cut off, a sample of the test object of the right ear gallery is washed off by normal saline, a dry cotton ball is used for wiping, two round lugs are chiseled at the same part by a puncher, and the weight of the right lug minus the weight of the left lug is taken as a swelling value. The results are shown in Table 6.

As a result:

and (4) conclusion: the test results show that the examples 1-4 and the fluoxyfen-ethyl acetate cream group can obviously reduce the weight of auricles of mice with acute inflammation swelling, obviously inhibit the acute inflammation swelling of auricles of mice caused by dimethylbenzene, and show that the compound containing artemisinin, radix zanthoxyli and sodium copper chlorophyllin in a specific range has obvious anti-inflammatory effect on acute inflammation; the anti-inflammatory effects of examples 5-9 are superior to those of the blank control group, but are lower than those of examples 1-4, which shows that the values of artemisinin, radix zanthoxyli and sodium copper chlorophyllin in the toothpaste are lower or higher than those of the invention, or any one of the artemisinin, the radix zanthoxyli and the sodium copper chlorophyllin is absent, and the anti-inflammatory effects are not obvious.

In conclusion, the traditional Chinese medicine toothpaste prepared by compounding artemisinin, radix zanthoxyli and sodium copper chlorophyllin in a specific range has obvious analgesic and anti-inflammatory effects.

Claims

1. The application of a traditional Chinese medicine composition in preparing toothpaste is characterized in that the traditional Chinese medicine composition is added into the toothpaste as the only active ingredient with anti-inflammatory and analgesic effects in the process of preparing the toothpaste, the traditional Chinese medicine composition comprises artemisinin, a radix zanthoxyli extract and sodium copper chlorophyllin, and the toothpaste comprises the following components in percentage by weight:

0.001% of artemisinin, 0.1% of radix zanthoxyli extract, 0.1% of sodium copper chlorophyllin, 50% of calcite powder, 25% of glycerol, 2% of xylitol, 2.2% of sodium dodecyl sulfate, 0.8% of sodium carboxymethylcellulose, 0.25% of sodium saccharin, 1% of spearmint essence, 0.075% of propylparaben, 0.075% of butylparaben and the balance of purified water;

the artemisinin is obtained by extracting and purifying sweet wormwood herb, and is a pure product with the content of 98 percent; the radix Zanthoxyli extraction method comprises pulverizing radix Zanthoxyli into certain particle size, extracting with 10 times of ethanol under reflux for 3 hr for 3 times, and concentrating the extractive solution under reduced pressure to obtain extract or drying into powder; the sodium copper chlorophyllin is one of chlorophyll derivatives, is prepared from chlorophyll as raw material by extracting, separating, concentrating, saponifying, salinizing and purifying, and has a purity of 98.5%.

2. The application of a traditional Chinese medicine composition in preparing toothpaste is characterized in that the traditional Chinese medicine composition is added into the toothpaste as the only active ingredient with anti-inflammatory and analgesic effects in the process of preparing the toothpaste, the traditional Chinese medicine composition comprises artemisinin, a radix zanthoxyli extract and sodium copper chlorophyllin, and the toothpaste comprises the following components in percentage by weight:

0.5% of artemisinin, 1% of radix zanthoxyli extract, 1% of sodium copper chlorophyllin, 18% of silicon dioxide powder, 40% of glycerol, 5% of xylitol, 2% of sodium dodecyl sulfate, 0.9% of sodium carboxymethyl cellulose, 0.6% of guar gum, 0.25% of sodium saccharin, 1.2% of peppermint essence, 0.075% of propylparaben, 0.075% of butylparaben and the balance of purified water;

the artemisinin is obtained by extracting and purifying sweet wormwood herb, and is a pure product with the content of 98 percent; the radix Zanthoxyli extraction method comprises crushing radix Zanthoxyli into certain particle size, extracting with 10 times of ethanol under reflux for 3 hr for 3 times, and concentrating the extractive solution under reduced pressure to obtain extract or drying into powder; the sodium copper chlorophyllin is one of chlorophyll derivatives, is prepared from chlorophyll as raw material by extracting, separating, concentrating, saponifying, salinizing and purifying, and has a purity of 98.5%.

3. The application of a traditional Chinese medicine composition in preparing toothpaste is characterized in that the traditional Chinese medicine composition is added into the toothpaste as the only active ingredient with anti-inflammatory and analgesic effects in the process of preparing the toothpaste, the traditional Chinese medicine composition comprises artemisinin, a radix zanthoxyli extract and sodium copper chlorophyllin, and the toothpaste comprises the following components in percentage by weight:

2% of artemisinin, 2% of zanthoxylum nitidum extract, 2% of sodium copper chlorophyllin, 30% of calcium hydrogen phosphate powder, 10% of silicon dioxide powder, 18% of sorbitol, 20% of glycerol, 2% of xylitol, 2.2% of sodium dodecyl sulfate, 1% of sodium carboxymethylcellulose, 0.4% of carrageenan, 0.25% of sodium saccharin, 1% of peppermint essence, 0.075% of propylparaben, 0.075% of butylparaben and the balance of purified water;

4. The application of a traditional Chinese medicine composition in preparing toothpaste is to add the traditional Chinese medicine composition serving as the only active ingredient with anti-inflammatory and analgesic effects into the toothpaste in the process of preparing the toothpaste, wherein the traditional Chinese medicine composition comprises artemisinin, a radix zanthoxyli extract and sodium copper chlorophyllin, and the toothpaste comprises the following components in percentage by weight:

3% of artemisinin, 5% of radix zanthoxyli extract, 5% of sodium copper chlorophyllin, 15% of silicon dioxide powder, 20% of sorbitol, 20% of glycerin, 5% of xylitol, 2.3% of sodium dodecyl sulfate, 0.8% of sodium carboxymethylcellulose, 0.5% of carrageenan, 0.25% of sodium saccharin, 1% of spearmint essence, 0.075% of propylparaben, 0.075% of butylparaben and the balance of purified water;